LOCUS       DAYYNQ010000001        77324 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000001 DAYYNQ010000000
VERSION     DAYYNQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.001"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..820)
                     /locus_tag="ACHBJ1_000001"
     CDS             complement(<1..820)
                     /locus_tag="ACHBJ1_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM9428981.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
     gene            complement(891..1352)
                     /locus_tag="ACHBJ1_000002"
     CDS             complement(891..1352)
                     /locus_tag="ACHBJ1_000002"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9428982.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
     gene            complement(1316..1441)
                     /locus_tag="ACHBJ1_000003"
     CDS             complement(1316..1441)
                     /locus_tag="ACHBJ1_000003"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9428983.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(1644..2123)
                     /gene="rlmH"
                     /locus_tag="ACHBJ1_000004"
     CDS             complement(1644..2123)
                     /gene="rlmH"
                     /locus_tag="ACHBJ1_000004"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM9428984.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2551..3840
                     /locus_tag="ACHBJ1_000005"
     CDS             2551..3840
                     /locus_tag="ACHBJ1_000005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HFM9428985.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVTSFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(3879..4484)
                     /gene="ytpR"
                     /locus_tag="ACHBJ1_000006"
     CDS             complement(3879..4484)
                     /gene="ytpR"
                     /locus_tag="ACHBJ1_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM9428986.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(4689..5153)
                     /locus_tag="ACHBJ1_000007"
     CDS             complement(4689..5153)
                     /locus_tag="ACHBJ1_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9428987.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5175..5495)
                     /locus_tag="ACHBJ1_000008"
     CDS             complement(5175..5495)
                     /locus_tag="ACHBJ1_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9428988.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(5639..6718)
                     /gene="pepA"
                     /locus_tag="ACHBJ1_000009"
     CDS             complement(5639..6718)
                     /gene="pepA"
                     /locus_tag="ACHBJ1_000009"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM9428989.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            6918..7268
                     /locus_tag="ACHBJ1_000010"
     CDS             6918..7268
                     /locus_tag="ACHBJ1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9428990.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7491..8438
                     /locus_tag="ACHBJ1_000011"
     CDS             7491..8438
                     /locus_tag="ACHBJ1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9428991.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8495..9913)
                     /gene="pepV"
                     /locus_tag="ACHBJ1_000012"
     CDS             complement(8495..9913)
                     /gene="pepV"
                     /locus_tag="ACHBJ1_000012"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM9428992.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10062..10907
                     /locus_tag="ACHBJ1_000013"
     CDS             10062..10907
                     /locus_tag="ACHBJ1_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM9428993.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            10911..11351
                     /locus_tag="ACHBJ1_000014"
     CDS             10911..11351
                     /locus_tag="ACHBJ1_000014"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM9428994.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11401..11595
                     /locus_tag="ACHBJ1_000015"
     CDS             11401..11595
                     /locus_tag="ACHBJ1_000015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9428995.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            11731..12408
                     /locus_tag="ACHBJ1_000016"
     CDS             11731..12408
                     /locus_tag="ACHBJ1_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9428996.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(12816..13505)
                     /locus_tag="ACHBJ1_000017"
     CDS             complement(12816..13505)
                     /locus_tag="ACHBJ1_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9428997.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13480..15609)
                     /locus_tag="ACHBJ1_000018"
     CDS             complement(13480..15609)
                     /locus_tag="ACHBJ1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM9428998.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(15657..16994)
                     /locus_tag="ACHBJ1_000019"
     CDS             complement(15657..16994)
                     /locus_tag="ACHBJ1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM9428999.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17246..17605)
                     /gene="rplT"
                     /locus_tag="ACHBJ1_000020"
     CDS             complement(17246..17605)
                     /gene="rplT"
                     /locus_tag="ACHBJ1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM9429000.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(17658..17858)
                     /gene="rpmI"
                     /locus_tag="ACHBJ1_000021"
     CDS             complement(17658..17858)
                     /gene="rpmI"
                     /locus_tag="ACHBJ1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM9429001.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(17930..18454)
                     /gene="infC"
                     /locus_tag="ACHBJ1_000022"
     CDS             complement(17930..18454)
                     /gene="infC"
                     /locus_tag="ACHBJ1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM9429002.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18498..18623)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            18774..19406
                     /locus_tag="ACHBJ1_000023"
     CDS             18774..19406
                     /locus_tag="ACHBJ1_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9429003.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19408..20313)
                     /locus_tag="ACHBJ1_000024"
     CDS             complement(19408..20313)
                     /locus_tag="ACHBJ1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9429004.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20398..21057)
                     /locus_tag="ACHBJ1_000025"
     CDS             complement(20398..21057)
                     /locus_tag="ACHBJ1_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9429005.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21373..22317
                     /gene="mvk"
                     /locus_tag="ACHBJ1_000026"
     CDS             21373..22317
                     /gene="mvk"
                     /locus_tag="ACHBJ1_000026"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM9429006.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22310..23287
                     /gene="mvaD"
                     /locus_tag="ACHBJ1_000027"
     CDS             22310..23287
                     /gene="mvaD"
                     /locus_tag="ACHBJ1_000027"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM9429007.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23300..24385
                     /locus_tag="ACHBJ1_000028"
     CDS             23300..24385
                     /locus_tag="ACHBJ1_000028"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM9429008.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24382..25446
                     /gene="fni"
                     /locus_tag="ACHBJ1_000029"
     CDS             24382..25446
                     /gene="fni"
                     /locus_tag="ACHBJ1_000029"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM9429009.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(25695..26498)
                     /locus_tag="ACHBJ1_000030"
     CDS             complement(25695..26498)
                     /locus_tag="ACHBJ1_000030"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM9429010.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26518..27360)
                     /gene="kduI"
                     /locus_tag="ACHBJ1_000031"
     CDS             complement(26518..27360)
                     /gene="kduI"
                     /locus_tag="ACHBJ1_000031"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM9429011.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27376..28383)
                     /locus_tag="ACHBJ1_000032"
     CDS             complement(27376..28383)
                     /locus_tag="ACHBJ1_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM9429012.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28390..29034)
                     /locus_tag="ACHBJ1_000033"
     CDS             complement(28390..29034)
                     /locus_tag="ACHBJ1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM9429013.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29134..29892)
                     /locus_tag="ACHBJ1_000034"
     CDS             complement(29134..29892)
                     /locus_tag="ACHBJ1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM9429014.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30019..31170)
                     /gene="fucO"
                     /locus_tag="ACHBJ1_000035"
     CDS             complement(30019..31170)
                     /gene="fucO"
                     /locus_tag="ACHBJ1_000035"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM9429015.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31252..31563)
                     /gene="rhaM"
                     /locus_tag="ACHBJ1_000036"
     CDS             complement(31252..31563)
                     /gene="rhaM"
                     /locus_tag="ACHBJ1_000036"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM9429016.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31565..32410)
                     /gene="rhaD"
                     /locus_tag="ACHBJ1_000037"
     CDS             complement(31565..32410)
                     /gene="rhaD"
                     /locus_tag="ACHBJ1_000037"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM9429017.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32424..33680)
                     /gene="rhaA"
                     /locus_tag="ACHBJ1_000038"
     CDS             complement(32424..33680)
                     /gene="rhaA"
                     /locus_tag="ACHBJ1_000038"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM9429018.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(33677..35137)
                     /gene="rhaB"
                     /locus_tag="ACHBJ1_000039"
     CDS             complement(33677..35137)
                     /gene="rhaB"
                     /locus_tag="ACHBJ1_000039"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM9429019.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35290..36159
                     /locus_tag="ACHBJ1_000040"
     CDS             35290..36159
                     /locus_tag="ACHBJ1_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9429020.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36285..37184)
                     /locus_tag="ACHBJ1_000041"
     CDS             complement(36285..37184)
                     /locus_tag="ACHBJ1_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429021.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37349..38173)
                     /locus_tag="ACHBJ1_000042"
     CDS             complement(37349..38173)
                     /locus_tag="ACHBJ1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9429022.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38184..39479)
                     /locus_tag="ACHBJ1_000043"
     CDS             complement(38184..39479)
                     /locus_tag="ACHBJ1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM9429023.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39495..39974)
                     /locus_tag="ACHBJ1_000044"
     CDS             complement(39495..39974)
                     /locus_tag="ACHBJ1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM9429024.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(39980..41008)
                     /locus_tag="ACHBJ1_000045"
     CDS             complement(39980..41008)
                     /locus_tag="ACHBJ1_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM9429025.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41025..42131)
                     /locus_tag="ACHBJ1_000046"
     CDS             complement(41025..42131)
                     /locus_tag="ACHBJ1_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9429026.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42360..44957)
                     /gene="adhE"
                     /locus_tag="ACHBJ1_000047"
                     /gene_synonym="adhC"
     CDS             complement(42360..44957)
                     /gene="adhE"
                     /locus_tag="ACHBJ1_000047"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM9429027.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(45262..45642)
                     /gene="yajC"
                     /locus_tag="ACHBJ1_000048"
     CDS             complement(45262..45642)
                     /gene="yajC"
                     /locus_tag="ACHBJ1_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM9429028.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(45739..46884)
                     /gene="tgt"
                     /locus_tag="ACHBJ1_000049"
     CDS             complement(45739..46884)
                     /gene="tgt"
                     /locus_tag="ACHBJ1_000049"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM9429029.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47102..47800
                     /locus_tag="ACHBJ1_000050"
     CDS             47102..47800
                     /locus_tag="ACHBJ1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9429030.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(47968..50607)
                     /locus_tag="ACHBJ1_000051"
     CDS             complement(47968..50607)
                     /locus_tag="ACHBJ1_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9429031.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51226..52257)
                     /gene="queA"
                     /locus_tag="ACHBJ1_000052"
     CDS             complement(51226..52257)
                     /gene="queA"
                     /locus_tag="ACHBJ1_000052"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM9429032.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            52450..53625
                     /locus_tag="ACHBJ1_000053"
     CDS             52450..53625
                     /locus_tag="ACHBJ1_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9429033.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            53629..54270
                     /locus_tag="ACHBJ1_000054"
     CDS             53629..54270
                     /locus_tag="ACHBJ1_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429034.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVIILALLADWLLGLLEHRLTPIALREENEE"
     gene            54267..55184
                     /locus_tag="ACHBJ1_000055"
     CDS             54267..55184
                     /locus_tag="ACHBJ1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9429035.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55181..55852
                     /locus_tag="ACHBJ1_000056"
     CDS             55181..55852
                     /locus_tag="ACHBJ1_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429036.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(56393..56881)
                     /locus_tag="ACHBJ1_000057"
     CDS             complement(56393..56881)
                     /locus_tag="ACHBJ1_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM9429037.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57082..57348)
                     /locus_tag="ACHBJ1_000058"
     CDS             complement(57082..57348)
                     /locus_tag="ACHBJ1_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9429038.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(57348..57611)
                     /locus_tag="ACHBJ1_000059"
     CDS             complement(57348..57611)
                     /locus_tag="ACHBJ1_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9429039.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58132..58272
                     /locus_tag="ACHBJ1_000060"
                     /pseudo
     CDS             58132..58272
                     /locus_tag="ACHBJ1_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(58414..60237)
                     /locus_tag="ACHBJ1_000061"
     CDS             complement(58414..60237)
                     /locus_tag="ACHBJ1_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9429040.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(60835..63540)
                     /locus_tag="ACHBJ1_000062"
     CDS             complement(60835..63540)
                     /locus_tag="ACHBJ1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM9429041.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(63846..64793)
                     /locus_tag="ACHBJ1_000063"
     CDS             complement(63846..64793)
                     /locus_tag="ACHBJ1_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM9429042.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            64983..65252
                     /gene="rpsN"
                     /locus_tag="ACHBJ1_000064"
     CDS             64983..65252
                     /gene="rpsN"
                     /locus_tag="ACHBJ1_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9429043.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(65428..65790)
                     /locus_tag="ACHBJ1_000065"
     CDS             complement(65428..65790)
                     /locus_tag="ACHBJ1_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9429044.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(65801..66922)
                     /gene="alr"
                     /locus_tag="ACHBJ1_000066"
     CDS             complement(65801..66922)
                     /gene="alr"
                     /locus_tag="ACHBJ1_000066"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM9429045.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(66938..67288)
                     /gene="acpS"
                     /locus_tag="ACHBJ1_000067"
     CDS             complement(66938..67288)
                     /gene="acpS"
                     /locus_tag="ACHBJ1_000067"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM9429046.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(67418..68929)
                     /locus_tag="ACHBJ1_000068"
     CDS             complement(67418..68929)
                     /locus_tag="ACHBJ1_000068"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9429047.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(69251..70603)
                     /gene="murF"
                     /locus_tag="ACHBJ1_000069"
     CDS             complement(69251..70603)
                     /gene="murF"
                     /locus_tag="ACHBJ1_000069"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM9429048.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(70756..71832)
                     /locus_tag="ACHBJ1_000070"
     CDS             complement(70756..71832)
                     /locus_tag="ACHBJ1_000070"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM9429049.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72028..73773
                     /locus_tag="ACHBJ1_000071"
     CDS             72028..73773
                     /locus_tag="ACHBJ1_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM9429050.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74053..74490)
                     /locus_tag="ACHBJ1_000072"
     CDS             complement(74053..74490)
                     /locus_tag="ACHBJ1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM9429051.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(74474..75847)
                     /locus_tag="ACHBJ1_000073"
     CDS             complement(74474..75847)
                     /locus_tag="ACHBJ1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429052.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76169..76657)
                     /locus_tag="ACHBJ1_000074"
     CDS             complement(76169..76657)
                     /locus_tag="ACHBJ1_000074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429053.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(76679..77281)
                     /locus_tag="ACHBJ1_000075"
     CDS             complement(76679..77281)
                     /locus_tag="ACHBJ1_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9429054.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22216 a        16479 c        14092 g        24537 t
ORIGIN      
        1 tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg atatttctag
       61 atatatggac acaacattgt tgaaattgag catttggata aacactatga atactatcgt
      121 caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca cggtctttta
      181 gatcttgaag aatctctttc caaacataag cagattcagt tggagctata gtaaatccta
      241 gaacctcttt agttccatcc aacctgatac caattactat atagacagct tcttttgata
      301 cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag tatttagctt
      361 ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc atatttgaaa
      421 tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag atttcggaca
      481 tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca ttggttcttt
      541 tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt attactaaat
      601 tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg gaattacctg
      661 aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat tcagcttgta
      721 ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa tcacctttat
      781 tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc tcttttctgt
      841 gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat ttacacaaaa
      901 tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact aggatttgtg
      961 ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga ataattgcgt
     1021 tctatatata tttggtattt attaagttgc cctccagact ccgaagacag aattttattt
     1081 tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat attcttattt
     1141 tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata attgatagca
     1201 gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa aaactcatca
     1261 gataaattgt gggtttcatt tggagcaaac aaaaaagcta aaaaattact atgtcttatt
     1321 tcatttttag atagcttaag tatctgaaat atatttgttt tcccagtcca tttagatagt
     1381 tttcttcaat atcatccata tcctctaata gattttttaa acactttttt tcatctatca
     1441 tgttttttct ccttgccatt attctaacta aaattttacg tatttgtttc tatgtctaca
     1501 ttatcttttt aacagtatct atttttcttt tgcaacttac ttggctacca aagcaaataa
     1561 aaaagttatt tataaatttc tccctaccac tacctattgc tattatttaa aatagggata
     1621 attttttctc ttaaaaaata tcattatttg tggtatggag cccccgcatt gatccggaag
     1681 gcacggtaga tttgctcgac aaggatcaga cgcatcaatt gatgtggata cgtcattttt
     1741 ccaaaagaga tctgagcatt gcttcttttc atgacggctt cgctgagtcc taaagagccg
     1801 ccgataacaa aaacaaggtg gctttttcct tggataccta attggtcgat ttgtttagca
     1861 aaagattcac ttgtaggatt tttcccttca attgctaagg catagacgta ttcttgttct
     1921 ttgattttcg ctaagatccg ttccccttct ttttctttta cttgtctcat ttgcgcttcg
     1981 cttaaattct ccggggcttt ttcgtcagga acttcgatca attcgatttt tgcataggca
     2041 ttcagccgtt tgacatactc attgatacct tggatcaggt atttttcttt taattttcct
     2101 actgagataa ttttgatatt catgttttct ctcctttggt cttcatgctc actttgttta
     2161 ttataatcaa ttttttcgtc tatatgttta ttttggctat ttttgttaaa aagatttccc
     2221 caacagttat gcacaacatc cacaggttta tccacagaaa acagtgttat tttaccttat
     2281 ttcatcacat tcattaggca aacatacgtt ttataagaat ttatccactt tatacacagg
     2341 ctgtggatat gttttatccc ttatttttcc aatcctatcc gcgtagtttt acacagtatc
     2401 tgtggaaaaa gaaaatcaaa tattttcatc tgaaaacaat gatttttcct aaagtttttc
     2461 ataaaaagtt cacagtctca aagtagacta aagtcaacaa ataagactat actaacagta
     2521 gataaatgaa tgtaattgga ggaggaacac atggatagaa aaaatgtgac accaaagatg
     2581 aaaaaaagta aaaataatag cctctggcgt aagttaggtc tggggcttgt tggagggatc
     2641 gttggaggac tactgacagc tggcattttt tatgcagcga tgggcactgg caacaatagt
     2701 tctactgcta caagcggcaa tcagaattct gccggtgaaa cagttgtgga aaatgtcaaa
     2761 gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc tgtcgtttct
     2821 gttatcaact tacaaagtca aaaccaatca agcggttttg gcggactatt cgggcagcaa
     2881 gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg aagcggcgtg
     2941 atctacaaaa aagacgggaa tactgcttat gtagtcacga ataatcacgt agtagatggc
     3001 cagcaaggtc ttgaagtctt gatgaaagac ggtacgaagg taaaagctga attagttgga
     3061 acagatgctt attcagattt agcggtgttg aagatcaatt cagacaaagt cgaaacagtt
     3121 acctcttttg gtgactcaag tgctctgaag gttggtgagc ctgcgattgc gattggttcc
     3181 ccactaggat ctgaatatgc aaattctgtc acttcaggaa tcatttcttc cttgaaccga
     3241 caagtgacaa gtacgaatga atcgaacgaa acagtcaata tcaatgcgat ccaaacagat
     3301 gcagcaatca accctggaaa ttctggtggt cctttagtta atatcgaagg acaagttatc
     3361 ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaatgt aagcgtagaa
     3421 ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca actggaaaaa
     3481 gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc tgccgtttct
     3541 actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg tgtcatcgtg
     3601 acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata cgacgtcatt
     3661 actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc tttgtataag
     3721 aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga aaaaacaact
     3781 actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa
     3841 taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa tgcttctccg
     3901 actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt tggcaaatgc
     3961 aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt tcctggccag
     4021 atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt ttgtccagct
     4081 tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac ttctgtctca
     4141 gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta cccgacaacg
     4201 atcttcggtt catgatcagc ttcaagctgt tcttcgaatc ctgcttcttt gatcaatcga
     4261 ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc gattgttaga
     4321 tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc tttgatccga
     4381 gcaacattcc atttgcgttc aacagtgttt tctgttcctt tatcgtcggc tataataacc
     4441 atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt tcctccatta
     4501 tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgaaat ttttttcatg
     4561 ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg gactgtgaca
     4621 agacttttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt cgtacaagtt
     4681 ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac atcacaaggt
     4741 gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac tgcatttaga
     4801 cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt gactgccatg
     4861 gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc aacagatttc
     4921 ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg atcgactaga
     4981 ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat tacgtttgct
     5041 acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa agcagcaagt
     5101 gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt catcttgacc
     5161 cctcctaaca gatgtcagcc ttttgttaca ctttctagga acgattcgat ttcctgtttt
     5221 gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat cacgacaaaa
     5281 cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc acggtctact
     5341 tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg tttgataaaa
     5401 cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt ttctacgtag
     5461 cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact ttctgtttat
     5521 gtatttttca attaaattat acacctttct ttcgaactaa caaacattga tcctcttttt
     5581 ataaaaaaaa tctggcaaaa agcaatcttt tgccagattt tatagtttca ctatcgcatt
     5641 attttccgta tacgatcgta tttactgttg atttatccaa acctttcagt gtttgaatca
     5701 acatttctcg tgcagcttca aaatcgcgaa tactgaacat cgtttgatgc gtatggatat
     5761 aacgtcccac tacgccaatc actgtacttg ggattccttc gttttgtgta tgagcagcac
     5821 ctgcatctgt accgcctttt gaaacaaagt attgataagg gatattgttt gtttcagcga
     5881 tatccagcaa gtactcgcgc aaacgaggaa gcatgatcaa acctggatct tggatacgca
     5941 tcaatgtacc ttctcctaaa tgtccgaatg tgccattttt cgtcactgta tcatctgctg
     6001 cagagcagtc tactgcaaag aacagatctg gcttgaattt attgacagaa gctttagaac
     6061 cacgcaagcc tacttcttcc tggacattcg cgcctgcaat caatgtatga cctaatgttt
     6121 cattttgcaa cgcttctaaa gcttctaata ccattgtaca gccatagcgg ttgtcccatg
     6181 atttactgat aatattcttt ccgttggctg ttttgatcgt ttcagcgtaa ggaacgattg
     6241 tgtctcctgg aaggacaccg taactcattg cctcttctct tgattcaaac ccagcatcga
     6301 acaaaatatc ggttacttct actgattttt gtccgcttgt tcctcgcaat aaatgtgggg
     6361 gaacggaaga agaaatacat ggataattct taccagtaga ggttttaagt gtaaaacgct
     6421 gagcagaaac aacgtaagga ttccagccgc ctagaggtac gactttaaaa agcccgtttt
     6481 cttggatttg tgtcagcatg aatccgactt catccatatg tgctgcgacc attacacggg
     6541 gtgcatccgc ttcttcatga tggcgcagac caaaaatgcc gcccaaacca tctaactgta
     6601 actcatctac cagcggttcc atatgacttt ccatataagc gcgaacatct tgttcaaaac
     6661 cgcttgttcc ttgtaattct gttaactctt tgattcgttg aaatgttttt tcttccatat
     6721 gctattcgtc taaatttcag actaaactcc tctcagactt atattgatct gcggataaaa
     6781 tatgattttt tctgcagacc gatcgtattt attatagctt attttttctg gtagcggtta
     6841 tttatttcaa agaaaatatg agagtgtggt aaaataagca caaggagtaa caagtgagaa
     6901 gaaggaggta tgtccacatg tatgaagaaa gcgaactaag ctattttaaa ggcgggttag
     6961 cagttggaat cggtctagga ttattgagcg gggtagcttc aacgatttgg tatcatcgca
     7021 aaaaagtatt gtcagcagac caagtacttg aaaatatcaa gtcatctttt ctaaaagaag
     7081 gtgcaatcga aggttcttgg atctcttttg aaaaggaacc tattaggaaa ttcgccgttc
     7141 ggtcgagtgc ttatcgggga ggtctttctc gaatagaaga tggcgaaact gtttattatg
     7201 aatttttagc agatgctcat acaggaaccg tattagaaat ttcaagaaaa aaaggtgcaa
     7261 cagattaata cgtcaaaaaa gtgagagaac gaatacgcac ccactattca aaagagaggt
     7321 atcatgtcct tcttttgtag tgggtggttt tttagataga gctttccaaa catatattcc
     7381 agtattttct ttttaaataa atgatattta gcgggaaaaa ctagcatttc ctttgattat
     7441 gaggtatttt taatttagct tttgttttct atagttaagg agagtgaaaa atggaacaac
     7501 aagaaaaaat tgctgttctg attccttgtt acaatgagga agcaacaatt tcaacagtca
     7561 ttgcagactt taaacgagaa ttaccagaag cagatattta tgtctatgat aataattcga
     7621 ccgatcgaac atacgaattg gccgttcaag gtggagcaat cgtaaaaaaa gaacctcgtc
     7681 aaggaaaagg aaatgtgatc cgccaaatgt tctttgatat cgatgccgac tattatttaa
     7741 tggtagatgg cgatgatact tatcctgcag aagctgtaca tggtttgctc gagaaattac
     7801 gttctggtga agcagatatg gtcattggtg atcgcctatc aaatggaacc tactttgatg
     7861 agaataagag acctttccac gattttggaa ataaccttgt tagaaataca attactagaa
     7921 tgtataaaac aaaaattctt gatgtcatga ccggctatcg aggattcaat cgtatctttg
     7981 tcaaaagttt tcctattatg agttctggtt ttcaaatcga aacggaattg acgatccatg
     8041 ctttagataa aaaattcaag ttcgttgaaa tcccaatcga ttatcgtgat cgtcctgaag
     8101 gcagcgaatc aaaattgaat acattctctg acggattcaa agtgatcatg atgattatta
     8161 aaatgtttaa agattataag cctttgcttt tctttgggat ttggaccatc ttcttctttt
     8221 tatttggatt ggtcacgggg attcctgtga ttcgcgaata tatgctgaca agctttatta
     8281 cacgcatccc ttctgcgatt ttaagtactg gattgatgac tttagcactt ctttcattag
     8341 taacagggtt gattttggat acagtcgtca cgaacgctaa gaaagaatat gaattgaatc
     8401 tatatcatgt ttatcatgaa taccacagta gaaagtaaaa aaaggatcaa gactgcaaaa
     8461 ataatttgca attcttgatc ctctttttaa ttaattattt cgccaaacga tagatggcat
     8521 ctgcatagat tgctgttgct ctgaacaagt catcaagact cataaattcg tttgcttgat
     8581 gcattgtatc agtatatcct gggaacatcg ctccataagc aacgccacgt ttcaacaaac
     8641 gtccgtaagt tccgccaccg atgatttgtt cgtagccttt ttctccagtg tggtcttcat
     8701 agacttgcaa taatgtagcc accaatggat catccattgg tacgtaatgt ggttccatta
     8761 cgcgagcacc tcgagtaact gttgcgcctt cttgccccac tgtttgttcg atgtcacttt
     8821 gcaatccgtc tactgttaca ccttttggaa aacggaaatt catattgatg aagttcgctt
     8881 cctctgtacc attttcttca aaagcaaata gtccagcatt cattgtcaat ttgcccattt
     8941 tttcatcttc ataagcgact cctagttttt ctccataaaa atcttcatgg acataagcag
     9001 ctgctacatg gataaattgt ttagcagatc ctaggaattc atagttatct aagaacaatg
     9061 ccaagaaaga acctgcattg ataccagatt gtgggcttgc accatgtgcg cctttaccaa
     9121 ctaattcgat ttttacatac gtattgtccg cttcaatcgt tccgcttact ggatttgctt
     9181 cgatgaattg gtaaaatgct tcttccatcg cgatagctgc atctgcactt ggtacttgaa
     9241 gtgctgcagt tgctgttcct ggcaccatat tttcgcgaag tccggaaaca aaagatttca
     9301 agacataatc tcctgcattg tcgcctttga agcgtaaagc taaagaaaca tttccttttt
     9361 caccattgat gattgggaat tcagcatctg gtgagaaacc aaaatctggt tcttcttcat
     9421 gttcgaaata ataagccata tcgccccagc cgctttcttc atcgctgccg actacgaagc
     9481 gaactttttt tgatagtttc aagtcaagtt ctttaatgat cttcatcgca tagtaagctg
     9541 ccatacttgg acctttgtca tcgcttgatc cgcgtgcaaa gatctttcca tccttgatta
     9601 ctggttcata aggatctgtg tcccagccat ctcctgcagg aacgacatcc atgtgtccaa
     9661 atatccctag cgtttcgtct ccttcgccaa gatcaatatg ccctgcatag ttatcgacat
     9721 tttttacttt aaatccatca cgttcgccat aagctaacat atgtttcaat gcgtcgcgag
     9781 gtccaggtcc aaatggtgca tctggtgtta cttttgagtc atcgcgctcg ctgttgacac
     9841 gtaaaagatt tttcaagtct tcaagcaagt cttctttacg tgcttctact tctttttgcc
     9901 aatcaattgt cataaagtta atcctccact tatttaagtc ttcgtgttca tgataccaca
     9961 aattacagaa aaaaatagtt caaaaaatct gacaattcct tttcttatct gtttttctct
    10021 aaaaaaaata tacgctataa tgagtaaaag gaggttttta gatgctgata aacgaacaac
    10081 tagtgaaaag agtcatcaca cctcgtccaa aaatttcaca taaaggaaca tttggacgta
    10141 tcgttttgat tggcggcaat gaacaatatg gcggagcaat cattatgagt gctttagctg
    10201 ccgtacacag cggagcgggt ctcacgactg tagtaactga tgaaaaaaat cggagtgctc
    10261 ttcacagtca tctgccagaa gcaatgattg tagtttggac ggatactgac cagtttcttt
    10321 ccgtaataaa gcaagcagat gtactcttga ttggtcccgg attgggtact tcttctaaga
    10381 gtttggaacg tttcaaacaa gtactagaaa accaatcaaa agaacaatgg ctggtgattg
    10441 atggctctgc tttaagttta cttgctgcta atcatctttt gctaccctat cccgaacaaa
    10501 ctgtacttac acctcatcaa atggagtggc aacggttgag ttctctaccg atacaagaac
    10561 aacgggataa agaaaacaaa aagaagcaac aggaaatcgg tgctgtgctc gttttgaaaa
    10621 gtcatgagac aaaaatttat ggtcaccagc acttctacac caatccttta ggttctgctg
    10681 ctatggcaac gggcggcatg ggtgacactt tagctgggat gatcacagga tttttagccc
    10741 agtttcctca aaatgaccaa acgattgcag cagctattta tcttcatagt ttcattggag
    10801 accagctggc aaaagaacaa tatattgttt tgccgacaca aatcagcgaa gcacttccga
    10861 aatggatgaa ttatttttgt aaaaaatcaa tggaaaacga ggcatgagtc atggagaaat
    10921 ttacaggtta tgtgcgagaa cctttttatt atgaaacaga tcaaatgggg attattcatc
    10981 acagcaatta tatccgctgg tttgaagagg ctcgggtagc cttattggta catctagggt
    11041 ttccctataa aaaaatcgaa gaatctggga tcatcatccc cgttctggaa gtaaattgca
    11101 gctataaaga aatgatccat tacggagaaa aagtgagcat ccatccaact atccaaaagt
    11161 atacggggac acggttggat tttagctacg aaattgtggg aactgaagac aaaaaattaa
    11221 aaactactgg cagtagtaaa cactgttttt tactttctga ttcgcaacga ttagtgaagc
    11281 tgagcaaagt caatccggag ttagatcagt tattccaagc gtacgcttct ttggatgatg
    11341 taaattctta ataaagaccg tctaagcatt tttcttgtat tgtgtcgagt agttcgtata
    11401 atgaaaagaa aaaagcaggt gatccccatg tatgtcgtta aagtaatgca cggttacatc
    11461 gataaaactg gctgtcgcac tcgggaaaaa aacttagaca atctgctcat tttcaaagac
    11521 aaaaaagaat ccgaagcttt tgctaaaaga atcggcggtc gtgtcaaacc gatccaagag
    11581 gtacgaccag attgattcaa tatttttctt ttcgaggctc taataggcat gcctatcagg
    11641 gctttttttg cagactgtgt ttttatgccc acacgatttg taagcgattt atgttataat
    11701 agattatatc tgcatagaaa ggaaggttaa atggttatca ataatttgca acagcaagct
    11761 tatgaatcta tacgtaaaca aatcatttat tccgagttag cacctggaag taaaatctcc
    11821 gataaagtgt tagaagaaca actagctatc gggcgtacac cgattcgaga agcactgatc
    11881 caactgcgca atcaagagct tgtaaaaacc attccacagt ccggcactta tgtttcaaaa
    11941 atcgatatcc gatctgcttc attagcccgt tatacgcggg aaaagttgga aaaaccgatc
    12001 ctgcaagaat gcagtgcgaa aatgaccaag caagatcaag ctaaactaga aaaaatacta
    12061 gaacaaaccg atcaggcagt aatcgctcaa aataaaaaag ctttttttga tttggatact
    12121 gattttcacc gaacttgtta tgaaattgct ggaaaacgag aaatttggga ttggctagaa
    12181 agttatagta cgcgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga
    12241 gtattagatg aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc
    12301 ggatttcttt gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat
    12361 aattaccccg attattttga agatattcag gacagaccca tcaaataaga ggttttggaa
    12421 tcagcgtttt gacctgagta ttagagaaag aaacggcaaa atagttcctc atattttgcc
    12481 gttttttttg agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg
    12541 aaaaagctgc cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt
    12601 taacttggct ggacttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc
    12661 gtgagcctgc cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt
    12721 ttgttgaata ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa
    12781 aaaaggactg tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc
    12841 gtacatcttt tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta
    12901 tatggccaaa gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct
    12961 tttgaaaaac tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa
    13021 aaaggcaacc gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct
    13081 tcgatgtggt gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc
    13141 gaagctagtc ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca
    13201 tcttctgcat aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg
    13261 attggaacag ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg
    13321 atgctacatg ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg
    13381 gacaaaggaa tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct
    13441 tcggggatct gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc
    13501 ggcaacgacg atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag
    13561 ctgtagttgt tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata
    13621 aaattctggt cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc
    13681 gacagctgtc atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac
    13741 cttgataaat cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg
    13801 ttcgccgcat acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa
    13861 tcgcatatcc tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc
    13921 ggttgctttc acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc
    13981 gatttctacg tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat
    14041 cgcttcattt ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac
    14101 aacatctcgg accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg
    14161 aatcgaatat ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc
    14221 ccctacacct acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa
    14281 aatcaccggt tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac
    14341 cgtcgtatga caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc
    14401 ataagaaaaa tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat
    14461 agtcacttct tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt
    14521 aggagagaaa aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata
    14581 atctagaccg gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc
    14641 ccgtaccatc gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt
    14701 tgggtggcca cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat
    14761 attggttgtt gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa
    14821 agagcccatg actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat
    14881 ttcattgatt tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc
    14941 tgcttctcgt acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga
    15001 tgcattgatt gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga
    15061 agccatcgtt gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc
    15121 cccattatct actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa
    15181 agaagaaatc gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt
    15241 gacaatctct atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt
    15301 tgctaaaaaa ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc
    15361 tccttcaatc agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc
    15421 tggactgata ttattttcca gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac
    15481 tccacggaca tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc
    15541 attatcgatt gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa
    15601 acgtttcatt ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag
    15661 gcgttcccgt acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt
    15721 gggatggatt tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca
    15781 acccaagatt gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg
    15841 aacaaaaagg ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag
    15901 cctgaaccga ctagattcat tggaccgcgg aaaaatccta gtggtgtcaa tatagcaaat
    15961 acgattgcca agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa
    16021 ggagaagcat acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt
    16081 ggcgcaacat ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta
    16141 taacctccgc gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct
    16201 aagatgattg gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat
    16261 gagatcattg gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc
    16321 tttttgttca ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca
    16381 atcataccaa tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca
    16441 gcataaatcg tttgatattg tttgaaattg acgatattag caaagattcc agccataatc
    16501 gagcccataa atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc
    16561 aacacaataa ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca
    16621 gcggttacaa tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact
    16681 acctttcgaa tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca
    16741 ccaaagaaga catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata
    16801 tgcgtcaaca catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt
    16861 ccgactagcg ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg
    16921 tagcctttta cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata
    16981 ataatatcac tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata
    17041 tcagtcaaac tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg
    17101 ttcagttttc aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg
    17161 tttcatttga tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct
    17221 tctttcctct ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca
    17281 atgcagtaaa tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt
    17341 cgatttcagc aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag
    17401 ctgctgcgtt gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat
    17461 cgcgatatgc gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt
    17521 gttttgaacc gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta
    17581 ctgttccacc tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat
    17641 tctgttattt gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg
    17701 ccactagaaa ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg
    17761 aaacggtgac ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa
    17821 cgttttgctg atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat
    17881 ctttcgttat ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt
    17941 cgtttttcgg tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca
    18001 ctgttgcaat atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt
    18061 tatgggtaat ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca
    18121 agaacttacg tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta
    18181 agcgaacttc ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt
    18241 gttgttcgaa acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag
    18301 gggcaactaa cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg
    18361 tcttaactcc taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc
    18421 cgtcgttaac catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag
    18481 aaaagcttac cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac
    18541 ttcacctaat agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga
    18601 agcgggtgct tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg
    18661 tcaatgtttt tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc
    18721 tagcagtgat tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa
    18781 aaatcgaacc tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag
    18841 atgaggagat cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca
    18901 aaaatatggt attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac
    18961 gtcattttga atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga
    19021 ttggtggcag tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg
    19081 ccgaaagtta tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta
    19141 gtagtacaaa cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt
    19201 atgaggtcac ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt
    19261 ttcgaacttc tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat
    19321 ctcaagattc tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag
    19381 cccttggttt gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat
    19441 ccaaatagat aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt
    19501 aaagggtaac tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat
    19561 cagcgaccag accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt
    19621 ataagtgcca gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg
    19681 tcgtgcttcg atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata
    19741 aatagccaat gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa
    19801 gtaaaccaaa ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc
    19861 tcttaatttc agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa
    19921 ggaatcagaa atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat
    19981 tcgttccaaa agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt
    20041 aaacaaaccg tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac
    20101 tttttgctgg tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc
    20161 tacgcgcgcc cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata
    20221 ttcttctttt aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt
    20281 acggtcttga ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca
    20341 gtataaagaa aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca
    20401 aatggctact ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc
    20461 ttcttttgct tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg
    20521 actttctttg tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc
    20581 gtatagactt ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc
    20641 caacatcgta tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc
    20701 agcataggga agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt
    20761 ttctgttgct cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga
    20821 acggtccaat acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt
    20881 caaaaaataa atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa
    20941 gacttcatta tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat
    21001 ttctgttaaa cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta
    21061 cctccagctc acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc
    21121 caatatctag tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa
    21181 ttcttaagaa aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg
    21241 gttttagtaa aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg
    21301 aagcagcgca actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga
    21361 aggagttaga ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg
    21421 ggcgagcacg cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa
    21481 gtcaccgcat tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat
    21541 tcaggaaatt tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac
    21601 ttaaaaaaac agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct
    21661 gctgaacgtg gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat
    21721 gattacttag catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa
    21781 aaaatcgccc atggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg
    21841 atttatttta caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg
    21901 attgtcgctg atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac
    21961 ctctttgaaa cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg
    22021 aagcaagcaa aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat
    22081 gaatcacaat cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc
    22141 caaacagcaa aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc
    22201 tgtatgattg cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa
    22261 gatgcgggtg ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg
    22321 caaagcacgc gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga
    22381 actcatcctt ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac
    22441 agaagtcatc ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca
    22501 agacgaaaaa gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg
    22561 cctctcacta aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc
    22621 ctcctctgcc agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg
    22681 attagatgat ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag
    22741 tattttcggt ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa
    22801 gccagtccct tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga
    22861 ccagaaaaaa gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt
    22921 ttatcaaggc tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa
    22981 aacaaaagat ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg
    23041 aacgacttta gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat
    23101 ggatgcggtg cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg
    23161 ccctaatgtc aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac
    23221 caaattattt agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat
    23281 tgaataaagg gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg
    23341 agaatatgca gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt
    23401 gacagtcact gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg
    23461 gatgcctgta cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc
    23521 ttttcattat atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat
    23581 tcttttatcc ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa
    23641 atatggattg ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt
    23701 ttacgcgttg aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc
    23761 agttcaagat aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc
    23821 tttctcaacc ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga
    23881 gttacttgcc ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt
    23941 acgtttattg attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt
    24001 tcaccgttcg agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga
    24061 atgtgtcaac gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat
    24121 gattcgaaaa aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga
    24181 aacgcctgct ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc
    24241 ttctggtgca ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct
    24301 tcctttaatg agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag
    24361 cgatcaaaga aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc
    24421 tttcatgata aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg
    24481 caaatagcag tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc
    24541 ccattttata tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat
    24601 cttgcaatcg tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc
    24661 ttgaaagtcc cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg
    24721 aaaatcattg ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga
    24781 ttgtttcacg cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct
    24841 gagggagacc gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt
    24901 gacgtccccc tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat
    24961 ctagctgctt taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc
    25021 cagatcgaaa acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa
    25081 tcgacagtct cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc
    25141 tccggaggta tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc
    25201 gtgggtactt caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt
    25261 atactgatga agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg
    25321 aataccgcgg ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc
    25381 gaagcaagag ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa
    25441 aagtaaagaa cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa
    25501 ctccttcgga aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt
    25561 cttatttctt ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta
    25621 atcagctgtc cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt
    25681 gtactaaagc tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac
    25741 aagacatggc cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc
    25801 aaatcgattg gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat
    25861 ggatgacgtt caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata
    25921 taacctggtc caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt
    25981 ttcgtcagca tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct
    26041 aattcactca tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc
    26101 ggaataactg cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg
    26161 aagtctgccg catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact
    26221 agaatatcaa ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct
    26281 tcatcggtca catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct
    26341 ataccttcat caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta
    26401 gctaaagagc gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact
    26461 tttccatcta aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta
    26521 ttttaattga tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat
    26581 tgcccagatg aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga
    26641 aataatggct tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat
    26701 gtggaacaca cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg
    26761 ttcatgtgta tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc
    26821 catttgtaat tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc
    26881 attcagtgtc aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac
    26941 atttggatat ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc
    27001 atcttgagaa gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt
    27061 tttcatttct tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac
    27121 acctagttca cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc
    27181 taattctttt tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt
    27241 caaactgatt ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc
    27301 cgttgaataa tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat
    27361 tttcttctac tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac
    27421 ttcttcttga ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc
    27481 cgtagcaaaa tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc
    27541 acctgcaaaa gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg
    27601 ggattccaca tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga
    27661 atgaacggta cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt
    27721 ttgataataa taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa
    27781 ataaagagca tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat
    27841 tacttcgctg gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat
    27901 caggcagcca gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt
    27961 agacaaagct ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc
    28021 tagcgaccat tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac
    28081 tgaggcggcg cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc
    28141 agataatacc tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc
    28201 caatgcgtta tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga
    28261 aattgctacg tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat
    28321 acgggttccc tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc
    28381 cataagcagc tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt
    28441 tactttgtca aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt
    28501 tacgacacca gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat
    28561 gatattgact tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct
    28621 tccaggaaat agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat
    28681 agtcatacat cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg
    28741 atcaaaggaa gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac
    28801 agcgtctaat actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa
    28861 aagttctttg attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag
    28921 accacctttg atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac
    28981 caccgcaatc actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc
    29041 cacctatttc ctatttagaa attttatttt ataacttgga aatctatacc catattatac
    29101 ggcagaaaag aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc
    29161 gctttttgtt tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata
    29221 tttcggcata cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc
    29281 tatacaaaaa atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac
    29341 cgttttgatt tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat
    29401 caacggatgc ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc
    29461 tttgcccatc gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat
    29521 cgtttgatgt tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc
    29581 taagtggata gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc
    29641 taccaaatcg atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg
    29701 atagtttttc tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt
    29761 caagactgta gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg
    29821 gttttccgct aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt
    29881 tttattttcc atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca
    29941 tcaatttaaa agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt
    30001 tgaagatccg cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt
    30061 cgtttctctt ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg
    30121 aatatctgct tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct
    30181 caaccgatcg atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc
    30241 tcctttaatc cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt
    30301 atatttcatg acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca
    30361 tgcacttaac gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat
    30421 tcccgcaata tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga
    30481 agcttccaaa gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc
    30541 ccaagcgcct tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga
    30601 tgcagccaat tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg
    30661 gatatcatga ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta
    30721 gttgatcgtt acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca
    30781 tggatttttc gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt
    30841 caaaataatc ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa
    30901 acagtctgcc tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg
    30961 attaggtacg atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt
    31021 tacttttgtt gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc
    31081 gaatcctcgc tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga
    31141 tgtttcattt aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt
    31201 cacaataaaa acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc
    31261 aaagactggc atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc
    31321 ttccatgtac ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt
    31381 ttcatcttcg atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga
    31441 atagtcgatg accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg
    31501 tctttttttg tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac
    31561 catgtcagac acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac
    31621 caaacgcatc tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt
    31681 gcgctttgac gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga
    31741 cttcatccat gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag
    31801 tttctttcat tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg
    31861 gaatgatgga aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg
    31921 tacgactaaa ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag
    31981 catggcaatg cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca
    32041 tgaaatgact tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt
    32101 ccagtgtttt gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg
    32161 ctacattttt aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc
    32221 tggcatcaaa aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga
    32281 tctcttcttc ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc
    32341 gataaagatt cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta
    32401 tttttttcaa tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc
    32461 tggacttctt ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc
    32521 catacatcgg catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag
    32581 tcaaaagcca gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt
    32641 gctttttgtg tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca
    32701 aagaaatcca gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg
    32761 atttcctgca attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg
    32821 gaaacatgca acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc
    32881 acttctgttg gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg
    32941 taacccatat aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt
    33001 ttgctttcta ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg
    33061 agtgattcca ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca
    33121 tcaggtaccc aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg
    33181 attttgcggc ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga
    33241 gccaatgtaa agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga
    33301 ccgatttctt ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca
    33361 tttagatcga cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg
    33421 cccggtatca acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg
    33481 gctcctggaa aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag
    33541 aaacctttga catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt
    33601 tctagtacag catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa
    33661 tttctttcaa tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg
    33721 tgttttgctt attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat
    33781 ttcttgcttg ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc
    33841 cgattgctgt ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca
    33901 attgattcag tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg
    33961 ttccagttaa gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat
    34021 acacacaacg agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt
    34081 attgctgaat ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca
    34141 tcacttgctg cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact
    34201 cgtactcttg cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa
    34261 aacgcaccgt gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag
    34321 aaacattggc gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac
    34381 cccaacgttc tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta
    34441 ctgtgataaa ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc
    34501 ctagtatcgt tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt
    34561 caagaagttc tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat
    34621 tagttttctc cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta
    34681 acaatttatg tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat
    34741 tgaaaggctg gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag
    34801 aaaactgttc tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc
    34861 gatccccttg ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa
    34921 ggcgatcaat ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat
    34981 aatcgatctt ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta
    35041 cttcttccaa gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg
    35101 atgcaccgat atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt
    35161 gttgattagc actttattaa taaagcgtta tcatttattg acaatgctat cataatacag
    35221 aatcttatat tttgcttcac actattttgg tatataattg cattatcttg acaagttggg
    35281 agtgtttaca tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga
    35341 gtgatcttga cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt
    35401 tacttcaaag caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata
    35461 aagtttactt tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa
    35521 gctggagact tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa
    35581 gaaccatggg aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag
    35641 gagttcacaa aagacggcca taacaccttc cattcaacag gagatccatc agaaaacaag
    35701 atccaccatt tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat
    35761 ttccagtcct ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag
    35821 ctagatttcg aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac
    35881 gaacatttca aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa
    35941 tatcagctga gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc
    36001 agcaaaaaac tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg
    36061 atttccgaag aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa
    36121 acaggactat cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat
    36181 tttttcaaaa tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta
    36241 tgtaaacaac agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca
    36301 aaatgttcat aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt
    36361 atgccgaact ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta
    36421 ctatatacag cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt
    36481 ttcttcatta catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata
    36541 tggtatttgc tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa
    36601 ttttttgaga tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt
    36661 tcttgcgcac tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga
    36721 gcttgttcat attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat
    36781 gataattttt gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc
    36841 attggttgaa aacaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa
    36901 ggggtgacaa agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc
    36961 aaatcattgg aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc
    37021 aaataaaaag tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga
    37081 tagatcgttt tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa
    37141 accatcgtat tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt
    37201 tgttcactta tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt
    37261 atatactctt ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa
    37321 atattagtta accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc
    37381 catcatcttt tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt
    37441 ctttgatttt tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct
    37501 gttcgacacg aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag
    37561 cttcttctac caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat
    37621 ctaaaatgat tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta
    37681 aagaatacac cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc
    37741 cagcttttac caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat
    37801 tttcttccgt atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg
    37861 caccaaagga agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta
    37921 tcgttaaatc tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt
    37981 gcttttctaa aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag
    38041 aaggtatttc aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt
    38101 ttgttttttc tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg
    38161 taatgttcaa cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc
    38221 gggaagtaag taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt
    38281 ttcagcattg cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct
    38341 actggcggcg tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca
    38401 ggatcaaccc ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca
    38461 ggcgccacat ccataaacat acccaccagt aataacacaa gattgactaa taacagaata
    38521 ataaatttat tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta
    38581 aacgccatgg cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac
    38641 attgctactg attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa
    38701 aacatagcaa tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat
    38761 atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct
    38821 tttattattt ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt
    38881 ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa
    38941 cctcccataa acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa
    39001 gcagtacttg gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca
    39061 aacttccggt catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta
    39121 gaagcggcaa tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta
    39181 tgagccaaag cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta
    39241 gctatccctc cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc
    39301 gaaaaactat tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac
    39361 ggtataacta acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc
    39421 cctaatacaa gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt
    39481 ttattcctcc atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt
    39541 agattgatca ggctatagat aatgatgagt acccccgata caggtactga cagataaaca
    39601 tatcccatag gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc
    39661 catccgccat acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact
    39721 tctaaaattt tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt
    39781 gctttttgtc ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga
    39841 acaagctctt ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat
    39901 aaaacaacag cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt
    39961 aaaatcattt tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt
    40021 aggtaggatc gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat
    40081 tatctacatc attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa
    40141 ttgcttttgc aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg
    40201 ctttttcctg ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg
    40261 gcacacgtgt atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat
    40321 acaagacaaa ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca
    40381 cttcatcact tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg
    40441 tttcgctttg catcacacgg atcttctttc ccttcaaatc ttccggcgta tggatcggac
    40501 cgtctaccat ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta
    40561 aatttttggt cgattggtag attggctcca ttatttcttc attatccatc accttaaaga
    40621 aatgttcttc atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac
    40681 tttccaacgc tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga
    40741 tcagttcacg ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat
    40801 ctgttttttc ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt
    40861 tgcttgcata agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg
    40921 ccacactaga attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg
    40981 ctgcaaacag cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg
    41041 agcaattctg tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg
    41101 atattccgat aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact
    41161 tctccttttt catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta
    41221 tattcttgtg gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca
    41281 gttgcagaag attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt
    41341 tcagactgat aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg
    41401 cgcaatgcat cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc
    41461 aaccagcagt ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc
    41521 cagccgtgat accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac
    41581 tggtaacgag cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa
    41641 ggaagcactg tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat
    41701 gtggcatgct gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc
    41761 cattgttcta aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata
    41821 gtcaatagcg gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc
    41881 tcaaaccatt cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta
    41941 taaataccat atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct
    42001 actttcagtc catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg
    42061 ttttcaacga gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct
    42121 ttaataatca tctttacgcc acctttttaa acgctttcat tttatatcct taatatagca
    42181 gtaataccgt ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa
    42241 tgttaaataa caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac
    42301 aacgttttcc aagttctttt tcatctattt tcttatcttt tttctacgct ttcttttgct
    42361 tatccgttgt aagcatcaat gatgatctgt ttcaattcag agatcaatgg ttctttcgga
    42421 ttagctgtcg tacattggtc ttcgtaagct aattcagcca tacggtctac ggttgtatct
    42481 agtgtttctt gtgtgactcc ttgagctttt agactcatgt tgatgccgac agattttcct
    42541 aattcataaa cagccgttgc taaagcttcg actaattcct ctgttgtttc gcctttcaga
    42601 cctaagaatt ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc atatttaggg
    42661 aacatcgcat gtttttgtgg atcttttgca ttatagcgga taatatgcgg taaaagaatc
    42721 gcatttgtcc gtccatgagg gatcccgtat tccccgccga ttttatgtgc gactgagtga
    42781 caaataccta ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt
    42841 ttttcacgtg attccatatc aggtgttttt actgattttt ctaaatagtc aaagactaac
    42901 ttgattgctt gtaggctcaa tccacgtgta taatctgaag ccatgactga aacataggat
    42961 tcgattgcat gtgttaacac atccatcccg gtatctgctg tgacagatgc tggaactgac
    43021 atgacgaatt gaggatcgac gattgcgaca tctggcgtca atgcataatc tgctaacgga
    43081 tatttcacat gtgtttcact gtcagtgatg accgcaaatg gcgttacttc agaaccagtc
    43141 cctgatgttg tcgggataca tacgaattgt gttttttctg gtttgtcgat cttatatgtc
    43201 cgtttacgga tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca
    43261 tagaacatcc acattccctt agctgcgtcc attgcagaac cgcctccaag agcgattact
    43321 gtatctggct tgaagtctgc catcagtttc gttcctgcgt atacggtatt tgtcgatggg
    43381 ttcggttcaa catctgagaa gacttcgatc ttcacatcat ttttacgtcg tgccaatact
    43441 tcacgaactg tatcgcagta accgaattgg accataccag ggtcacaaac gatcatgaca
    43501 cgttcaacgt tttccatttt ctctaagtac aatagagagt tcttttcaaa gaagattttt
    43561 ggcggtaatt tgaaccattg catgttattt ctccgtttcg ctacagtttt gacattgatt
    43621 aagtttacag cagagacgtt acgagaaaca gagttcttac cataagaacc gcatcccaaa
    43681 gtcaatgacg gaatcatttc gttatagata tcaccgatgc cgccttctgc agatggtgta
    43741 ttcactaaaa tacgacaagc tttcattcga agaccgaatt tgacatgcaa ctcttcgttt
    43801 tctgaatgga taactgctgt atgtcctaat cctccaagat tcaacatacc ttcacacaaa
    43861 tcaaacgcat ggtccgtgtt gttagctttg atcatggcaa gaactggaga aagtttttct
    43921 cttgatagcg gataatctgg gcctacgcct tccaattcag caatcagcat tttcgttcct
    43981 tttggtacac tgattcctgc caattttgcg atttccatcg cagaatgtcc aacgattgat
    44041 ggattgaccg catattttcc ttcgttcatc actgcatctt ctaatttttg caattcatct
    44101 ggttttacga tataaacttg gtgtgcttgg aattctgctt ttactgctgc atagatttct
    44161 ttatccacga tcacagcttg ttcagaagca caaatcattc cattatcaaa tgttttcgaa
    44221 acgatcaaat cgttcactgc acgtttaatt tttgctgttt tttcaatata agctggtacg
    44281 ttacctggac ctacacctaa agctggtttt cctgtagagt aagcagactt gaccattcca
    44341 gctccacctg tcgctaagac gatcgcgata cctggatggt tcattagcat agaagtcgct
    44401 tcgatcgaag gatgttcgat ccattggata caattttcag gagctccggc agcgatagca
    44461 gcatcacgta ccactcgtgc tgcttctgca gaagattttt gggcacttgg atggaaagca
    44521 aaaacgattg gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc
    44581 ggattcgttg ttggagttac tccgcaaacc acaccgactg gttcagcaat ttcaatcaac
    44641 cctttttgga catcttcatt gatcacacca actgttttat catgtttgat attatgccaa
    44701 atatattcgg acgcatacat atttttgatt gctttatctt cgtaaacacc gcggcctgtt
    44761 tcctctacag ccatcttcgc taatggcata tgttgatcca aagctgccat tgccatttca
    44821 tgcacgatat ggtctacttt ttcttgatca aagctttcca tttctttcaa tgctgcattt
    44881 gctttttctg ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt
    44941 tctattactt tagccatcat tatcctccta atgggtcatg gttatttgtt aaataattca
    45001 catgtatatc atacaatatt tatttttcta tgtaaacact tttttgtgat ttttttcaca
    45061 gttattttta aatataaaat tgctttattt acaggtgttt gtatagaaaa atatataaag
    45121 ttatttatta ttatttatat gacacacccg attattcaat attgagcaat atatcccatg
    45181 ctcttatctc tttctttttt gaataaaaaa tgaggtagga catctttctg ccctacctcg
    45241 tctttaaata agctttgata cttatttttc ttccttctct tctgtctctt ccagcgtttc
    45301 tgttgtttca gtttttactt caactgtctc agctggttct tctgttgttt caacagtagt
    45361 cgttgcatca tttgttacga cagtccctgg tttgaccgtt ttgattgctg cacgatcgta
    45421 ctcaaggaag atcccttcgc agtctaaaac aactgtccgt ttgtcattgt cgatttctga
    45481 aacaactcca tgtaatccgc cgatcgttac gactgaatca cctggtttca tgctatctaa
    45541 caagttttga cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa accacattgc
    45601 taatagcgcg ataaacatca caagcattgg taatgttccc atttttttca cctcactaat
    45661 tctgtatcta caaaaaacac tttagcagaa aaatgctgat aacactagtt ttttacttaa
    45721 tttttaaaag attttccttt agaagctttt tgcgttttct ttattgaacc cgtattcttc
    45781 aaaaaaggct tctctaaatt caagcaaatt atcatccatg attgcttgac gtacttgttt
    45841 cattagattc aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc
    45901 atcacatttg atcaaatggc ggatataggc acgtgtatag tttttacatg tataacaatc
    45961 acatttctca tcaattggac ggaaatcatg ggcatatttc gcatttttca ctactagacg
    46021 gccttgagaa gtcatacatg tgccatttcg agcgattcga gtaggcaaga cacagtcaaa
    46081 catgtctact ccacggataa caccatcgat caatgaatca gcagcaccga cacccatcaa
    46141 gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac gattcatttc
    46201 tgatttaggt tccccgacag ataacccgcc gattgagtaa ccagggaaat ccatcccgac
    46261 tagatctttc gcgctttgac gacgaagatc ttcaaaccct gctccttgga tgattccaaa
    46321 taatccttga cggtctggat tagcatgagc tttcaatcct cgttctgccc atcgagacgt
    46381 acgttctact gattttttca cgtagtcgta actttcatcg aatggtggac attcgtcaaa
    46441 actcatcatg atatcagagc ccaatttatt ttggatgttg attgcttttt ctggtgataa
    46501 aaacatacgc gaaccgttca agtgattttt gaaatgcacg ccttcttctt cgatattccg
    46561 catatcactt aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg gttgatccca
    46621 gttcataaat ttatgcagtc cgccagcttc ttctactaag tcttcaccag ggcgtagcca
    46681 taaatgatat gtattactta aaataacgcc agcacccatt tctttcaact cttctgggga
    46741 cattgttttt actgtagcta atgttcctac tggcatgaac attggtgtgg ggaaagttcc
    46801 gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt ttttctttct tgattaaacg
    46861 atagcgaatg gcaggttgcg tcattcattt ccctactttc tatataaaat atcttgacaa
    46921 ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt ccttctactt
    46981 tatttaatat tctttttttt atcctttttt tatgaataac aaaaaaaaga aaaacattat
    47041 gacagaaata ttttttgata catggtatat tggtgttatt aaagaaaagg agggaaaaat
    47101 catgcaatct tctgcagaat taaaacgcca agcaaaagag agtctaaaag gacgttgggg
    47161 acaagctgtt ttactaaatc taatccctac attgattggt attgctacta ttcttcttat
    47221 tatgattcct attatcttgt ttgccaccac catatatgat ggatcagcga gcgtctcaga
    47281 agcgacaact tcaggaggcg gtagttcagg aggcggtggc ggaattattt ccagcatcat
    47341 tagcgctttg ttcatgagcg gtatttcttg gacttatctt gatattttac gtggacaaaa
    47401 agatcagatc gaaccattca aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg
    47461 agtgatcagc ttagctgtgc tgatctcgat ctttacaaca ttatggggtt tgctattagt
    47521 tattcctgga atcattaaaa gctattctta ttctcaaagc tattttattt actacgatat
    47581 cgtccaagaa acaggacaaa cacctaaagt actggatacg atcactgcaa gtcgaaaatt
    47641 gatggatggt tataaaggac aacttttctg gttagatctt tcatttatcg gctggcatat
    47701 cttagcatta gcaactgctg gtattggtta tctatggctc agcccataca tttctgcaac
    47761 aaaagcagca ttttatgaac aattgccaaa agatatttaa aagagagtgt gacttgtgac
    47821 cgagggggtg gcaggagaac accgtttatc gagaggcagt aaaagaaaca ttcagcccat
    47881 tagcaaaaag gaggtgtgac aacattcatg tcacacctcc tttttgtata gttccagtaa
    47941 aaagaaaatc aacttagaaa cagttaatca ttcctcttct tctgcttccg gtaatcgatg
    48001 gaacttgatc agttcattca ccgcgattgg tacgatagat aaaatcccta ccaagaacca
    48061 gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac
    48121 agccgttgta atagctaatg ccagtaacgc catttctacc aaagctttat tggtcgctaa
    48181 attgactttg aagatcgatt ggctgctgcg tacattgaac acatgcagaa tcgatgaata
    48241 agctaagatc aagaatgcaa cagtttgacc gacatgctga cttgcgtcta cccatgggct
    48301 gatagtatcg acataactac ctaaataata accaaacaag gttacgatgg tgaagacccc
    48361 tgcatttatg gcaatttttt gcaataaacc tcgagcaaag atcccttcat ttttcggaat
    48421 gggtggctga tccatgatgc cgtgttccgc tggctctctt cctaaagcaa atcctggaag
    48481 tccatcagca actacattga caaacagtag ttgcacagct gtaaatggtg ctccccatcc
    48541 taacagcatc gcaatcaaaa caataaagat ctccgaaata ttacagctta gcaaaaagtt
    48601 gatagcttta cgaatattct gataaaccga acgtccttga ctgacagcgt ctacgatcgt
    48661 cgcaaagttg tcatccgtca aaatcatatc tgcggcactt tgcgccacat ctgttcctgt
    48721 gatccccatg gcacacccaa cgttacttgc tttcaacgct ggtgcatcat tcacaccatc
    48781 cccagtcatc gctacgactg cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc
    48841 ttctggcgtt acccgagcat agacagagag agctttgaca cgcgcatcta attcttcgtc
    48901 tgataattga tgcagttctg atcctgaaag tgcttctgac ggatcattca agatacctaa
    48961 ttctttcgct atggcactgg cagtcaccac atgatctcca gtgatcatga ctgttttgat
    49021 gccagccttt ttcgctcgct tgattgctcc tttactttct ggacgtggtg gatcgatcat
    49081 tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg
    49141 ttcttcgtca taagtagcat aaccaacagc aatcactctc agtgcccgtt taccaaagcg
    49201 atcattaacg atcgccgctt gttctacatc gcctgaacta aagcgcggag caagaacatc
    49261 gaatgctcct ttggtgattg agatgtattt ttcgcccatt tggtgaactg ttgtcatcat
    49321 ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga tatttttctt ctaattctgc
    49381 ctttgtatga taattctctt caacagccct tacgatggct acttctgttg gattaccttt
    49441 gataacaagg tcgtcatctt cttgctcgac cgtcacatct gtacatagtg cagccatttt
    49501 caatatttcc atcgcgctgt tcgtcattga atcttcgata ttcgttactt catcttcttt
    49561 gctccatacc cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca
    49621 gataacatta gcggttccta gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt
    49681 cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta acaataacag tcaaggtttc
    49741 cggtacagct gcaacagcca aggaaatcga agtcatgaac atctcaagga gtggttctcc
    49801 ttgcagttca ccaatcacaa atactaaagc cgcagccgct aaagcgatca tactgattcg
    49861 tttgcctagc tgattcaatc ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa
    49921 aagatccgcg atcttaccca tttctgtagc catgccaatg gcagtgacga tcgctttccc
    49981 acgacctgca gcaaccgtac atcctttaaa taccatatta agttggtcac ctaatgactc
    50041 atcttcttca gcaataaatg ctgcatcttt ttcaactgct tcactttctc cagtcaacgc
    50101 cgattcttct acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc
    50161 accggactct aaaacaagaa tatcacccac taccagttgc tctgactcaa cagtggtcac
    50221 tttgccgtct cgtatgacag ttgccgtttg tttattcata tcttgcaagg attccagtgc
    50281 tttttctgcg tttccttctt ggacaatcgc caataccgag ttgatcacaa cgatagcgat
    50341 gatcagcagt ccttcaaata aatccccatg ttctgtagca aatgcggtat aaaaggaaat
    50401 caccgctgct acaagaagga tgatcgttgt aaaatcggac aagcttcgaa agaatttttt
    50461 gatcatcgat tctttcggcg cctcatcgaa tttattcaat ccctgctttt ttcgccgttc
    50521 atccacttct tgtgaagaca acccagtatc tggatctgct tctgttacct tctttacttc
    50581 ttctattgtc tgcttgtatt cttccatcct attcctcctt taaaatcttc gtacaaaaaa
    50641 aggtacttat tttactataa catccttttt ttatttactt caaataaagg gcttatcgat
    50701 tatttgttaa gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta ccaaaggatg
    50761 ctattatata gatgtaagga aggaggatac catcaaatag acagaagaat ttctcgatag
    50821 aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc acccagacat gcgtgtagag
    50881 tacaaaccta taaatagtga atgcgtatgg atgtttggat cacgaagtat ccgaaacaag
    50941 aagtaatgcg atacgtaaca attgaataac gtaaacgtct gaaaattgaa taggaacaag
    51001 agatgtttca aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt
    51061 caacaccgtg ctttgcatgt atgatggaaa gttacaatca tttctgttta tttgaatcct
    51121 tccgaggtac aagaaagccg agcttatcga gagctcggct ttttctttgt cataaaatct
    51181 ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt tcacgaacat
    51241 cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt aagcagataa
    51301 agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt ttggcaaatg
    51361 gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc
    51421 agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg tttctaatgt
    51481 tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta cttggttcaa
    51541 tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt gttcttcaat
    51601 tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca gatagactaa
    51661 ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca atccagctgt
    51721 tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat ctgggtcttc
    51781 cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa gaatctctag
    51841 aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt cttctttcac
    51901 tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg cacgtttcgc
    51961 aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa ggacttctaa
    52021 atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc gtgtattatt
    52081 cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat gtttatcttc
    52141 catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg
    52201 tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc
    52261 gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc tgttggcaaa
    52321 atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac cctatttgta
    52381 ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa tagaaaaaga
    52441 ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct
    52501 gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat cggtacgagc
    52561 ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc aacacaaggg
    52621 aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt gcgacgtcag
    52681 atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg tgaaaatatc
    52741 actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac agctgaaaaa
    52801 atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca tgaattatct
    52861 ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca agatatcatc
    52921 ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt gcaagacctt
    52981 gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca tgacatggat
    53041 gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt
    53101 gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga
    53161 gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat
    53221 acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa
    53281 aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat
    53341 gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa
    53401 catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa
    53461 cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact
    53521 cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact
    53581 gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt
    53641 tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga
    53701 cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt
    53761 ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt
    53821 gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca
    53881 ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc
    53941 gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt
    54001 tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt
    54061 tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg
    54121 atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta
    54181 ataattttag cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg
    54241 atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt
    54301 tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag
    54361 acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg
    54421 ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta
    54481 caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca
    54541 caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt
    54601 ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctata
    54661 gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat
    54721 ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt
    54781 ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca
    54841 attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg
    54901 atattgttct tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg
    54961 aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat
    55021 tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg
    55081 aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag
    55141 cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat
    55201 gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca
    55261 atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat
    55321 tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc
    55381 gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg
    55441 cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa
    55501 gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat
    55561 ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat
    55621 catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt
    55681 cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac
    55741 atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct
    55801 tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg
    55861 taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat
    55921 ttttaaggca ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt
    55981 agctatttat ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc
    56041 caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact
    56101 cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg
    56161 catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat
    56221 tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc
    56281 atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat
    56341 tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac
    56401 tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt
    56461 aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc
    56521 ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg actttaactt
    56581 cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag
    56641 gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat
    56701 cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt
    56761 tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga
    56821 tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca
    56881 cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat
    56941 gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa
    57001 ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta
    57061 aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac
    57121 tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta
    57181 tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc
    57241 cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct
    57301 taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc
    57361 caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta
    57421 tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc
    57481 ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt
    57541 tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt
    57601 gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac
    57661 gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag
    57721 aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact
    57781 agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc
    57841 caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac
    57901 ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa
    57961 actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg
    58021 tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa
    58081 ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct
    58141 acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga
    58201 ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg
    58261 ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct
    58321 tatagagggc acctttagct gagaataaga caaaacagac cctctagctt caattaagag
    58381 ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt
    58441 gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt
    58501 atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt
    58561 atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc
    58621 aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa
    58681 ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac
    58741 gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt
    58801 cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa
    58861 acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac
    58921 gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc
    58981 aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct
    59041 ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa
    59101 cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa
    59161 tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg
    59221 atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa
    59281 agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac
    59341 gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc
    59401 tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc
    59461 taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa
    59521 gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa
    59581 tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc
    59641 ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat
    59701 cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg
    59761 aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta
    59821 gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt
    59881 caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt
    59941 ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata
    60001 tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca
    60061 gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat
    60121 agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc
    60181 atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt
    60241 ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca
    60301 agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa
    60361 aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt
    60421 tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc
    60481 aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc
    60541 catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt
    60601 aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa gctgttttta
    60661 tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg
    60721 ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt
    60781 tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg
    60841 aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg
    60901 atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc
    60961 tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg
    61021 agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata
    61081 gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac
    61141 attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac
    61201 agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc
    61261 taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac
    61321 gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt
    61381 agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt
    61441 gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg
    61501 tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag
    61561 atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat
    61621 atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg
    61681 cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc
    61741 attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt
    61801 gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat
    61861 tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa
    61921 aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat
    61981 ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag
    62041 tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat
    62101 attattaaca acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt
    62161 ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa
    62221 ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt
    62281 agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa
    62341 gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat
    62401 agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt
    62461 caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc
    62521 tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa
    62581 gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt
    62641 cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt
    62701 ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc
    62761 aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta
    62821 atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa
    62881 ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat
    62941 catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt
    63001 gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg
    63061 gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac
    63121 cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca gatcttctga
    63181 gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa
    63241 ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat
    63301 gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta
    63361 aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa
    63421 ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt
    63481 tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat
    63541 agttctcccc cttcttcttt tttcagctga attactttac catgtatttc atactttttt
    63601 caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt
    63661 aatctcttac atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa
    63721 agggaaagat aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa
    63781 aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt
    63841 atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc
    63901 tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga
    63961 aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc
    64021 tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg
    64081 aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct
    64141 ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct
    64201 ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca
    64261 tttttactgg atgcactggt taagattcgg attccctttt catggagaag ctggatcaga
    64321 cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc
    64381 gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct
    64441 tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct
    64501 ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca
    64561 tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc
    64621 aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg
    64681 aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt
    64741 tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc
    64801 atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa aatgtctttt
    64861 cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta
    64921 tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg aggaatttgt
    64981 tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg attgaacgct
    65041 atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc
    65101 ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg
    65161 gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca cataaaggtt
    65221 tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat gtaaaggagg
    65281 agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct tctagcagaa
    65341 atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt ttttatatgt
    65401 aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg cttcgatacc
    65461 cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca aatggcagac
    65521 cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga taacggagtc
    65581 tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct taggtttagc
    65641 catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac ctagattatt
    65701 ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg gagaaagatc
    65761 agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga tattctcttg
    65821 gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat tgatctgcta
    65881 tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact tttgttccga
    65941 caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc ccaatcgttt
    66001 cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc cagccatctg
    66061 cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat gtttctccat
    66121 agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa accagctcaa
    66181 gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta agtccgtaca
    66241 ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa gccgttgcac
    66301 tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact tctataaagc
    66361 gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa
    66421 ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc tcttttactt
    66481 cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa
    66541 gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg
    66601 tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg taaggaacca
    66661 tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact ccttcatcta
    66721 aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct gtttcaatag
    66781 ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc
    66841 ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact gctttcgttg
    66901 ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa ataatttgtg
    66961 caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca tgaggtgaac
    67021 gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga aaggtaactt
    67081 ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa cgcccgccta
    67141 aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca tcagaggtca
    67201 agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta ggaagttcga
    67261 ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa tttagaacac
    67321 tctcattcta ccaaattata accattttgc cacaaataag tccacaaata acaaaaatga
    67381 aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt gaacggatta
    67441 caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg ttattatttt
    67501 tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga cgattacggt
    67561 tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt ggcaatgggc
    67621 gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc gtttttagca
    67681 aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct gtttgcaggt
    67741 atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca gcacctaatt
    67801 gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca
    67861 agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc acagacatac
    67921 cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct ggatcttgag
    67981 ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt gcagcaacgt
    68041 ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt
    68101 tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct tctaatccgc
    68161 gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat tcaggtgttt
    68221 gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg cggacatagt
    68281 attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga tgtggatttt
    68341 tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag aaaagtaatg
    68401 tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag aagcccatat
    68461 tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt
    68521 ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt ggacgatctt
    68581 tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt acacgtactt
    68641 ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat tcacgtgttg
    68701 gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct aacattggta
    68761 atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg tcttttccag
    68821 ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt
    68881 cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc
    68941 tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa
    69001 gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta gcccctgtgg
    69061 caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc
    69121 agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca aaaccagtcc
    69181 aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt ttctgcaagt
    69241 ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag cccatgctgt
    69301 tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata gcatcaatca
    69361 atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt tggttcaatt
    69421 cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat ttctcaggat
    69481 cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat tccagcatat
    69541 ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta tctaatacca
    69601 gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca ccatttcctg
    69661 ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct tggcgtatct
    69721 caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca ttcgttacat
    69781 tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa tcggtttgtg
    69841 cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg aatgttgtga
    69901 ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga
    69961 gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg attccttctc
    70021 gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca gcttttggtt
    70081 gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt tccaaaacga
    70141 tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt
    70201 aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg gttagatctt
    70261 tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct actttttcca
    70321 agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga ataactggaa
    70381 aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct ccgtttgtga
    70441 tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg aataaacttc
    70501 cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt gcagagcatt
    70561 cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc tctcctaaaa
    70621 aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc acttccaagt
    70681 aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc actccagcct
    70741 cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat ggtatcgatt
    70801 cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca tattttccca
    70861 taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt cattcaggaa
    70921 taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc cacctaacat
    70981 cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg gcacttcggc
    71041 tggaatctgc atttcgattt tattattgat atattttgct tcataatcat agaatgctac
    71101 gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta atacagcaac
    71161 ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat actgataggc
    71221 tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc cgacactaga
    71281 acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac attgatcaaa
    71341 tactttttta ggattttctt tccattgatt cttaagtact ggtacataag gaacttgcgg
    71401 cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac atgcactggt
    71461 caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt ggatcgttcc
    71521 atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt ctttgatttc
    71581 gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga
    71641 aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt tgacccattg
    71701 tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa tggcatttaa
    71761 aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc catatagtaa
    71821 agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt actctcatat
    71881 tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc cagcttctaa
    71941 aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt ttataaaaag
    72001 ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag ccgcttggca
    72061 gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca gcaaaaaaat
    72121 cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa gataggacag
    72181 atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt caaaacatta
    72241 caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga aacagaactt
    72301 tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc ctctgcttct
    72361 atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt taagatccaa
    72421 caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg agcgatccct
    72481 ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact ccaagaagta
    72541 aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc
    72601 tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga cgctttttgt
    72661 tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca tttaatgaac
    72721 gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct gaaacaagaa
    72781 gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg ttttttccat
    72841 gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga acagacacaa
    72901 gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc ttttcgagcc
    72961 tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga ttttggtatg
    73021 atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt agcgctgtat
    73081 acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg
    73141 tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta ttatgattct
    73201 cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa
    73261 aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg gacattagaa
    73321 ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc gccttttgct
    73381 caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag cggattagac
    73441 ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca tctattagaa
    73501 atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga caaattattg
    73561 agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc tgcattgatc
    73621 gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt cgtttcctta
    73681 ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc aatcgatgca
    73741 ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc
    73801 ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg aaaattcggc
    73861 ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc
    73921 tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc ctgtttccct
    73981 gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac tatcttctgt
    74041 acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca tatgatcttt
    74101 ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact ttccactatg
    74161 ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc cagagtgatg
    74221 gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa
    74281 aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag ctccgcggtc
    74341 tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt ccacactggt
    74401 cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt cttttactgg
    74461 agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat aaatgataaa
    74521 cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag ataagcagtt
    74581 gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga tggattggct
    74641 ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac
    74701 tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga atagctgtat
    74761 cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag aaactaagca
    74821 gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa gaactcagtc
    74881 caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat ggtccgacta
    74941 tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa agtttttcta
    75001 atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc cctagcattc
    75061 catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc ttctggtgtc
    75121 ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca agatcaaaaa
    75181 gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca aagaatatca
    75241 aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg aaatccagtt
    75301 cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt gtataaatag
    75361 taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac aggagaaata
    75421 gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct tgcgtctttt
    75481 cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc cctagcaaaa
    75541 cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc aatgaaccag
    75601 caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca aatcgtagac
    75661 tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg
    75721 tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata aaaataaccg
    75781 tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt ttgagttttt
    75841 cgaacatgac aatggctcct taaacagtct tataactaat ctgctcttcg attttagcat
    75901 taaaggggaa ataaaccaat tttacagaat atattcttgt aaaaaaaaat acagaaagaa
    75961 gcataagctt ctttctgcat ttttacggta cttgattact caagcagaca agcccttctc
    76021 cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga taaagataga aatcaattat
    76081 cctgtgcctc caaaatcaat taggagctct gtaaaccaga gtccttctcg acccgtcgca
    76141 taaatttact ttaacacagt tcctatgttt atgcgagagc ttttttcatt ttataatcga
    76201 ccatttgaaa tccttgaccg atatctgaaa taagttcttc tgtaatctca aatccttcat
    76261 gcttataaac ctcgattgct tgatgattcc cctgattcac tcgcaaaaag atctctcttt
    76321 cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac tgaagaagtc aatccttgac
    76381 cacgtacttt atttagcaaa tataatttac ttaaataaat ttttccattt atctcttcat
    76441 aagcagtgta accgacagtc ttttcttcgc tttttaataa aaagtaggac acaccttctg
    76501 tcagttcttt ttgaatctgt ttttttgact gataagtttt cagcatgtaa tccacttgtt
    76561 tttgaccaat gatcggcaca aaaacttccg gccatacttc ttgtatgact gccatcagct
    76621 catccagttc attttctgtt acttttattt tttccataca cccacgtcct tttttacatt
    76681 ataaatgacc ttgcgcttga tggaataaaa agagcatgat catgctatac acacccaccg
    76741 accacggttt caagaaaatc acaatctgca taaattccct aaaagataat ttcgtcaaac
    76801 cagctactag acagatcaag tcgtctggcg caaagggaag cagcaaggta acaactagta
    76861 cttttttaat atctttcgtt tttcttgtca gcaattcatc aaatttctga tacttttttg
    76921 gcgataaaat aagctggaca aaggctacgc catagtaacg gacaagcaaa aatccgattg
    76981 cttccccaat cactaggcca atacaactat ataaaagccc aaatccattg ccaaacatca
    77041 gcatgcccgc tactgaggag atccctcccg gcaaaatagg aatgatcact tggatcactt
    77101 gcaacagaac aaatatcatc actgcatact tgccaaattg ctggataaac gcttgcaaag
    77161 acgtgctcga tcgaaaaatc ccatgatgat aagcataaag aatcaacaca aagaaaaaaa
    77221 acagaccaat aatgggcatg atctgaagac atttttgtaa aactgatcct tttttagaca
    77281 tctatattcc ccacatcttt tttcatttat taaaactgcc tcct
//
LOCUS       DAYYNQ010000002        75672 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000002 DAYYNQ010000000
VERSION     DAYYNQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.002"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..775
                     /locus_tag="ACHBJ1_000076"
     CDS             <1..775
                     /locus_tag="ACHBJ1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9429055.1"
                     /translation="VTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQF
                     VEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVIN
                     RKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHYD
                     EILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITS
                     ISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            951..1694
                     /locus_tag="ACHBJ1_000077"
     CDS             951..1694
                     /locus_tag="ACHBJ1_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429056.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1687..3267
                     /locus_tag="ACHBJ1_000078"
     CDS             1687..3267
                     /locus_tag="ACHBJ1_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM9429057.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3518..3997)
                     /locus_tag="ACHBJ1_000079"
     CDS             complement(3518..3997)
                     /locus_tag="ACHBJ1_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9429058.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4013..4765)
                     /locus_tag="ACHBJ1_000080"
     CDS             complement(4013..4765)
                     /locus_tag="ACHBJ1_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM9429059.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4882..4952
                     /locus_tag="ACHBJ1_000081"
     tRNA            4882..4952
                     /locus_tag="ACHBJ1_000081"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4914..4916,aa:Gly,seq:ccc)
     gene            5313..6038
                     /locus_tag="ACHBJ1_000082"
     CDS             5313..6038
                     /locus_tag="ACHBJ1_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM9429060.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            6293..6559
                     /locus_tag="ACHBJ1_000083"
     CDS             6293..6559
                     /locus_tag="ACHBJ1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM9429061.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            6556..6987
                     /gene="ruvX"
                     /locus_tag="ACHBJ1_000084"
     CDS             6556..6987
                     /gene="ruvX"
                     /locus_tag="ACHBJ1_000084"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM9429062.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            7012..7317
                     /locus_tag="ACHBJ1_000085"
     CDS             7012..7317
                     /locus_tag="ACHBJ1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM9429063.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(7398..8543)
                     /locus_tag="ACHBJ1_000086"
     CDS             complement(7398..8543)
                     /locus_tag="ACHBJ1_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429064.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(8604..9251)
                     /locus_tag="ACHBJ1_000087"
     CDS             complement(8604..9251)
                     /locus_tag="ACHBJ1_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9429065.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            9445..9738
                     /locus_tag="ACHBJ1_000088"
     CDS             9445..9738
                     /locus_tag="ACHBJ1_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429066.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            9782..10093
                     /locus_tag="ACHBJ1_000089"
     CDS             9782..10093
                     /locus_tag="ACHBJ1_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429067.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            10090..10714
                     /locus_tag="ACHBJ1_000090"
                     /pseudo
     CDS             10090..10714
                     /locus_tag="ACHBJ1_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            10855..11013
                     /locus_tag="ACHBJ1_000091"
     CDS             10855..11013
                     /locus_tag="ACHBJ1_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429068.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            11014..11376
                     /locus_tag="ACHBJ1_000092"
     CDS             11014..11376
                     /locus_tag="ACHBJ1_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429069.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            11413..12261
                     /locus_tag="ACHBJ1_000093"
     CDS             11413..12261
                     /locus_tag="ACHBJ1_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM9429070.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            12251..13726
                     /locus_tag="ACHBJ1_000094"
     CDS             12251..13726
                     /locus_tag="ACHBJ1_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM9429071.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            13992..14399
                     /locus_tag="ACHBJ1_000095"
     CDS             13992..14399
                     /locus_tag="ACHBJ1_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM9429072.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            14402..14617
                     /locus_tag="ACHBJ1_000096"
     CDS             14402..14617
                     /locus_tag="ACHBJ1_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429073.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            14621..15001
                     /locus_tag="ACHBJ1_000097"
     CDS             14621..15001
                     /locus_tag="ACHBJ1_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9429074.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            15135..15290
                     /locus_tag="ACHBJ1_000098"
     CDS             15135..15290
                     /locus_tag="ACHBJ1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM9429075.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            15359..15832
                     /locus_tag="ACHBJ1_000099"
     CDS             15359..15832
                     /locus_tag="ACHBJ1_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9429076.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            15829..17523
                     /locus_tag="ACHBJ1_000100"
     CDS             15829..17523
                     /locus_tag="ACHBJ1_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9429077.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            17489..17674
                     /locus_tag="ACHBJ1_000101"
     CDS             17489..17674
                     /locus_tag="ACHBJ1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429078.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            17678..18853
                     /locus_tag="ACHBJ1_000102"
     CDS             17678..18853
                     /locus_tag="ACHBJ1_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9429079.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            18846..20369
                     /locus_tag="ACHBJ1_000103"
     CDS             18846..20369
                     /locus_tag="ACHBJ1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9429080.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            20425..20709
                     /locus_tag="ACHBJ1_000104"
     CDS             20425..20709
                     /locus_tag="ACHBJ1_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9429081.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            20696..21031
                     /locus_tag="ACHBJ1_000105"
     CDS             20696..21031
                     /locus_tag="ACHBJ1_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9429082.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            21326..21952
                     /locus_tag="ACHBJ1_000106"
     CDS             21326..21952
                     /locus_tag="ACHBJ1_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429083.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            22694..23530
                     /locus_tag="ACHBJ1_000107"
     CDS             22694..23530
                     /locus_tag="ACHBJ1_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM9429084.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            23594..24025
                     /locus_tag="ACHBJ1_000108"
     CDS             23594..24025
                     /locus_tag="ACHBJ1_000108"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429085.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(24087..25409)
                     /locus_tag="ACHBJ1_000109"
     CDS             complement(24087..25409)
                     /locus_tag="ACHBJ1_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM9429086.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(25563..27203)
                     /locus_tag="ACHBJ1_000110"
     CDS             complement(25563..27203)
                     /locus_tag="ACHBJ1_000110"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM9429087.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            27536..28006
                     /locus_tag="ACHBJ1_000111"
     CDS             27536..28006
                     /locus_tag="ACHBJ1_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM9429088.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            28228..29331
                     /locus_tag="ACHBJ1_000112"
     CDS             28228..29331
                     /locus_tag="ACHBJ1_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM9429089.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMTPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            29615..30295
                     /locus_tag="ACHBJ1_000113"
     CDS             29615..30295
                     /locus_tag="ACHBJ1_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9429090.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            30385..31419
                     /locus_tag="ACHBJ1_000114"
     CDS             30385..31419
                     /locus_tag="ACHBJ1_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9429091.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            31433..31768
                     /locus_tag="ACHBJ1_000115"
     CDS             31433..31768
                     /locus_tag="ACHBJ1_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429092.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            31752..34232
                     /locus_tag="ACHBJ1_000116"
     CDS             31752..34232
                     /locus_tag="ACHBJ1_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429093.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            34288..35847
                     /locus_tag="ACHBJ1_000117"
     CDS             34288..35847
                     /locus_tag="ACHBJ1_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429094.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            35844..37208
                     /locus_tag="ACHBJ1_000118"
     CDS             35844..37208
                     /locus_tag="ACHBJ1_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9429095.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            37379..38449
                     /locus_tag="ACHBJ1_000119"
     CDS             37379..38449
                     /locus_tag="ACHBJ1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429096.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            38464..39132
                     /locus_tag="ACHBJ1_000120"
     CDS             38464..39132
                     /locus_tag="ACHBJ1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429097.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            39152..39730
                     /locus_tag="ACHBJ1_000121"
     CDS             39152..39730
                     /locus_tag="ACHBJ1_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9429098.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            40131..41042
                     /locus_tag="ACHBJ1_000122"
     CDS             40131..41042
                     /locus_tag="ACHBJ1_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9429099.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(41420..42337)
                     /gene="rnhC"
                     /locus_tag="ACHBJ1_000123"
     CDS             complement(41420..42337)
                     /gene="rnhC"
                     /locus_tag="ACHBJ1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM9429100.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            42539..42976
                     /gene="zapA"
                     /locus_tag="ACHBJ1_000124"
     CDS             42539..42976
                     /gene="zapA"
                     /locus_tag="ACHBJ1_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM9429101.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            43034..43582
                     /locus_tag="ACHBJ1_000125"
     CDS             43034..43582
                     /locus_tag="ACHBJ1_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFM9429102.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            43697..46057
                     /locus_tag="ACHBJ1_000126"
     CDS             43697..46057
                     /locus_tag="ACHBJ1_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM9429103.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            46144..46458
                     /gene="trxA"
                     /locus_tag="ACHBJ1_000127"
     CDS             46144..46458
                     /gene="trxA"
                     /locus_tag="ACHBJ1_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM9429104.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            46640..48418
                     /gene="uvrC"
                     /locus_tag="ACHBJ1_000128"
     CDS             46640..48418
                     /gene="uvrC"
                     /locus_tag="ACHBJ1_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM9429105.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            48563..49462
                     /locus_tag="ACHBJ1_000129"
     CDS             48563..49462
                     /locus_tag="ACHBJ1_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429106.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            49455..50717
                     /locus_tag="ACHBJ1_000130"
     CDS             49455..50717
                     /locus_tag="ACHBJ1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429107.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            50878..52776
                     /locus_tag="ACHBJ1_000131"
     CDS             50878..52776
                     /locus_tag="ACHBJ1_000131"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM9429108.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(53106..53825)
                     /locus_tag="ACHBJ1_000132"
     CDS             complement(53106..53825)
                     /locus_tag="ACHBJ1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM9429109.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    54015..54062
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            54162..54494
                     /locus_tag="ACHBJ1_000133"
     CDS             54162..54494
                     /locus_tag="ACHBJ1_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429110.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            54481..56484
                     /locus_tag="ACHBJ1_000134"
     CDS             54481..56484
                     /locus_tag="ACHBJ1_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM9429111.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            56503..56973
                     /locus_tag="ACHBJ1_000135"
     CDS             56503..56973
                     /locus_tag="ACHBJ1_000135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM9429112.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            56994..57578
                     /locus_tag="ACHBJ1_000136"
     CDS             56994..57578
                     /locus_tag="ACHBJ1_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFM9429113.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            57593..58579
                     /locus_tag="ACHBJ1_000137"
     CDS             57593..58579
                     /locus_tag="ACHBJ1_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM9429114.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            58569..58880
                     /locus_tag="ACHBJ1_000138"
     CDS             58569..58880
                     /locus_tag="ACHBJ1_000138"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM9429115.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            58902..60683
                     /locus_tag="ACHBJ1_000139"
     CDS             58902..60683
                     /locus_tag="ACHBJ1_000139"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM9429116.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            60676..62052
                     /locus_tag="ACHBJ1_000140"
     CDS             60676..62052
                     /locus_tag="ACHBJ1_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM9429117.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            62059..62694
                     /locus_tag="ACHBJ1_000141"
     CDS             62059..62694
                     /locus_tag="ACHBJ1_000141"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM9429118.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            62724..64079
                     /locus_tag="ACHBJ1_000142"
     CDS             62724..64079
                     /locus_tag="ACHBJ1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9429119.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(64271..65665)
                     /locus_tag="ACHBJ1_000143"
     CDS             complement(64271..65665)
                     /locus_tag="ACHBJ1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9429120.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            65757..66728
                     /locus_tag="ACHBJ1_000144"
     CDS             65757..66728
                     /locus_tag="ACHBJ1_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM9429121.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            66725..67507
                     /locus_tag="ACHBJ1_000145"
     CDS             66725..67507
                     /locus_tag="ACHBJ1_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM9429122.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(67676..68047)
                     /locus_tag="ACHBJ1_000146"
     CDS             complement(67676..68047)
                     /locus_tag="ACHBJ1_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9429123.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            68181..68624
                     /locus_tag="ACHBJ1_000147"
     CDS             68181..68624
                     /locus_tag="ACHBJ1_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9429124.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(68667..69476)
                     /gene="yidA"
                     /locus_tag="ACHBJ1_000148"
     CDS             complement(68667..69476)
                     /gene="yidA"
                     /locus_tag="ACHBJ1_000148"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM9429125.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(69495..70862)
                     /locus_tag="ACHBJ1_000149"
     CDS             complement(69495..70862)
                     /locus_tag="ACHBJ1_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9429126.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(70846..71676)
                     /locus_tag="ACHBJ1_000150"
     CDS             complement(70846..71676)
                     /locus_tag="ACHBJ1_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM9429127.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            71839..72276
                     /locus_tag="ACHBJ1_000151"
     CDS             71839..72276
                     /locus_tag="ACHBJ1_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM9429128.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            72352..72972
                     /gene="rpoE"
                     /locus_tag="ACHBJ1_000152"
     CDS             72352..72972
                     /gene="rpoE"
                     /locus_tag="ACHBJ1_000152"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM9429129.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            73161..73667
                     /locus_tag="ACHBJ1_000153"
     CDS             73161..73667
                     /locus_tag="ACHBJ1_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9429130.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            73684..74583
                     /locus_tag="ACHBJ1_000154"
     CDS             73684..74583
                     /locus_tag="ACHBJ1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM9429131.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            74670..75554
                     /locus_tag="ACHBJ1_000155"
     CDS             74670..75554
                     /locus_tag="ACHBJ1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9429132.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        25140 a        12886 c        15743 g        21903 t
ORIGIN      
        1 ggtaacgcgt atatttattt tggatgatag tttggttttt gaaaaaatgc tagtagaaat
       61 tttggaagaa tccagaattt tattaagtcc ttggaaattt gagatcgttc agggattgaa
      121 cgttatacaa tttgttgaat tcattaaaaa taatgaaatc tattcctcag atattttttt
      181 cctagacatc gatttgaacg cctattatag cggtatcgaa ctaggcgaaa tgcttagaga
      241 aaagaatgaa aagtgtaaaa ttgtttatct aacgtctcat ccgaataaag caataactgt
      301 tattaatcga aagattacgc ctagtggtta tttgataaaa gaattcactt atgaaaaaaa
      361 taaaaaaagc atccaaagtt acttattgaa agaaaaagaa aactatcctg ttcaacatgc
      421 tacgaaaaaa aggtggacaa ctgtccgtta tggtaatagc gatcactata tccattacga
      481 tgaaatcctt tatctaatga gcatgccagg ttataaaaat aaaataggcc tagtattgaa
      541 agatgaagag attctcgtaa acggcacaat taaaaaatat aaaaaatcac tcgaatatga
      601 gtatttatgt aaagatttaa aagcttatat cattaatact gaaaacatta catctattag
      661 cagagtaaac gaaatagtaa catttgcaaa tggagaacaa ctagagatgg gaaagagaag
      721 tattgataaa ctaaagaatt ttatgcaaga aaagatagaa aatgaatcat tttaaagaaa
      781 acaaagactc atatcttacc tgttagaaga gtgttttcct gtagaagaga acaacccaag
      841 tagcagccta gtcgtttcta tccttttttg ttatgcactc tttttgacat ttactccacc
      901 aataacgaag ggtacaataa aaagtaataa caaacaaaag gagtacagaa atgattgaat
      961 tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt aaaagacttg tctctctcga
     1021 taagacaagg ggaaattttt ggatttttag gacataatgg tgccggaaaa tcaactacga
     1081 ttaaaagttt agttagtatt attgaaccat catcgggaac gattttagta gatggaatga
     1141 aattgacgga aaatcgattg agtatcaaac aaaaaatcgg ttatgtgcct gattccccag
     1201 atattttttt acaattgacg gctggagaat attgggactt gatttcagct gcctatgaat
     1261 tagacacaca aaaaaaagaa aagcgtttag cagaattgac agcgcttttt gatatgtata
     1321 gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat gcgtcaaaaa acgattttga
     1381 tcggcacgtt gctgccagat ccagatattt gggtattgga tgaacctttg caaggacttg
     1441 atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc tcatgccgca aaaggaaaaa
     1501 cagtcatttt ttctacacat gtattggata ccgcacaaca gttatgcgat aaactggcta
     1561 ttttgaagaa aggtgaattg atttatcagg gggcagttag tgaattattg aatggctcac
     1621 ctgatgaaac gctagaaaag atttatttga aaatggctgg aaggcaagcg acaggagatg
     1681 gaccgtatgt ataagcgaca attattagaa ttaattcgag ttaatctttt atatgttaat
     1741 ccacagctca cgaataaagc aagagagaaa ggaaaaaagg gaaagcgatt aactatctat
     1801 cttttgtctc aaaatctttt ttcagggctg atttttttac tgatttacgg atttgctatg
     1861 tttgctatga actttgttaa attgccaggt ttctttactt attacgtggc actttttggt
     1921 atgttaggtt taactcaaag tatatcagtc atttataata tcttttttga aggaaatgac
     1981 ctgcaagtat ttttgcctct gccttttgga caagaacagg ttttttcagc aaagattttg
     2041 gtagttgctt tgaccatcat tccttttgtt tttccgcttc tagtcatgtt tattttaacc
     2101 ggatggcgag ctgatctagg gattttgctt actatcatta tctcaatcct tttgttttct
     2161 ctatttttga ttattctttt ttgtctggct agtttgattg tttttggttt ggctcgaaca
     2221 gcagtgttta aaaaacatag aaaaacagtt tctagtatct tgcttggcag ctcgatgctg
     2281 atcgcatttg ctggaatcat gtggatgaat catcaaacgg ggactattga aaatgagtta
     2341 acagatcggc atccgatttc gatattattg ccattctatg atgctgccag tcagccactt
     2401 tctcaacaag gtttattggg ttggggaata ctagctatca cggctctggg atttttcctt
     2461 gctgtaaaac agctgatagt accaaagctt acagagcaac tgactgattc aaacagtgag
     2521 ttcaaaataa aaagaaccca taaaaagaac caaaatgtgc atcagctttt atttagttac
     2581 aatgctcaat tagtaaaaga acctaatttg attatgcaag ttctttcttc ttccttgtta
     2641 actccaatca tttttattct tgcttttgcc ctaggaggcg aaataaaatt aactgattta
     2701 gataataagt taatcggtgt tgttttcctg gtagggatcg cattggcttt cttaacagtt
     2761 aatcagactt cttttatcag caatcttatt tctttggatc aagaaaattt tctgtttatc
     2821 cgttctttgc caatctctat gaaccaatat ttaaaagaaa agttcagatt cggatggata
     2881 ttgcagagtt ttttaactgg tgggatcgca ttattaagcg gactgtcttt tcagctgcct
     2941 ttgtttttta tccctagctt gctatttggt tctttattcg gctgctactt attgtcacta
     3001 cgttatttcg ctagagatta tcggttgctg ctgctgaact ggaccaatat taaccaatta
     3061 tttaacagag gttcaggaag tttgggttta gtcgcaacta tgatgggttc attgatcatc
     3121 agtattattc ttatcgtctt atacggaatg ttggctttgt tttatccatt ttggttagtg
     3181 aatattcctg tgatcggttt agttctaata ctttccgctc tatggatcgt ttattatcaa
     3241 cgaacgtttt ggaaaaaatt tgaataaaga agaggggccg tgacaaaaat catgtcgcag
     3301 cccctttttt acagatagag ggtgttcaaa agtcagtttc tcagtattaa gtcaatttta
     3361 tgtaaaaatg tgagaagcta tttttacaaa acttttttta atactcataa ttacttctta
     3421 ttgctcctgc ggttacttgt cgcaatgttt gtgagatatt ttgtcatcgc tcaaggctga
     3481 acgtctcttt cacaaccttt tctttgttac acttcgttta tttaaaaagc cgtttttttc
     3541 gtttcatcat gattgcgtca cgctttactt tgaaattacg gatgttatgg ttaaagactt
     3601 ctgataatac aagacctaaa gcgattgctc cagcaatcat aattgcttgg accgcataat
     3661 gagccgcttc tcgataggat tcagtaacga gatttcgaac cgcttgataa gctaaaccac
     3721 cagggactag aggaaccatc ccgggaatat tgaaaatcgt tacgggcatt ttcaaacgtt
     3781 tagagaaaag atcgctgatg aatgctactc ctaaagaacc aagcaatgaa ccaaaagcag
     3841 cagatccgcc tatttgaaca cagatccagt agatcatcca gccaaaagca ccagaaagac
     3901 cacaacaaat caatgaacgc ctaggtacat ttgtaataac ggcaaatgcc gcagtcgcta
     3961 aaaaactaaa agaaaactga ataaatatcg tccccatact tgtgttcctt tcctaataaa
     4021 aaagctggaa aataaaagca atcgcaaaac cgatcatcgt agcagtcatc atcgcttctg
     4081 ttccgcgaga aactcctgag acataatgtc cagctagcaa atcacggata gcgttcgtaa
     4141 tttgaactcc aggaactaaa ggcatcacac atccgataat gattagatcc tgattgcttc
     4201 ctaagccaaa acgtacgctg tagatagcgg ctatgccaat caataaagaa gataaaaatt
     4261 ccgataaaaa tttgattcta aaaaatttca aactatatag ataagctgcg tatcccaatc
     4321 ctccaatgac taaagtcaca ggtaaatcgg tcatctttcc gccaaataac accataattg
     4381 ttccactgat cactgccgcg ctaacgattt gcaaccagac aggaaagaaa gaacggtctc
     4441 ggtcgattgc ttgcagttcg cagtaaagtt cttctaatgt tagctctgaa gcaacatatt
     4501 tacgtgaaag atcatttact tttactactt tttctaaatt aatcgaacgg ttggtaattt
     4561 gttccattcg gatggtcgag gtacgatcaa gaccaataaa gagaccggtt tgagtgacat
     4621 agctgaccaa acggaaattt ccagaagcta aagcaattcg actcatcgta tcttctaccc
     4681 gatacatctc cgcattgctt tccatcatga ttttcccagc gagtaaacaa gtgtccagca
     4741 acagatctaa ttcttttccc gtcatagcat cttctccaat aattgattga ttagttttat
     4801 tttactacaa aacataccag aagcgtaaag aataaaaaaa cttgaggaag gaataaaaga
     4861 ggcgtatact gtttttgtat tgcgggtgta gtttagtggt aaaatcccag cttcccaagc
     4921 tgatgtcgcg ggttcgattc ccgtcacccg cttaaaatag gttgtgaaaa agctgctctg
     4981 caacaagtaa taagaacagc caagcaaaaa caacttctta tgtttttaac ttggatggac
     5041 ttattaccgc agatgcaagc ttttgcgcac cgtttaccaa gcggttgtga aatcagtgta
     5101 ttgtcttgag tatgaagaaa atttttgaat aataataaat accttacaga tagagtggtg
     5161 actgattaga gacgaggaat ccgtctcttt tttgtatttt taagccatct taaaacaggt
     5221 tttatttttt gctgtttcta tttctttcta gtatttattt ataggggaac atatgttaag
     5281 atttagggag ctgttagtaa aggagtaaag aaatgagcaa aaaaacaacc aaggcgatcg
     5341 tggggatcgt cgctgctgtg attgcagggg cattcggtgt ttccaccaca caaaaagatt
     5401 cagatatctt tcagcagatt acaactattt ttgattccac accacaagct tctcaagtag
     5461 cggatgtgcc agaatatgat ggaaaacatc aagaaataga aatcaatggg aatcagccgg
     5521 aatttacaaa agaggatctt agcctttcca aaggaacatg ggaaagctat tcaaatattg
     5581 ataaattgaa cagagtagga caagccaatg ctatgcttgg aaaagatttg ttccctactg
     5641 aaaagagaga gcctttgtat attgacccga caggttggaa acaaaagaaa ttaagtgacg
     5701 ggcaatggtt atataatcgc agccatttaa ttggtttcca gctgactggt caaaataaca
     5761 acattaaaaa cttgatgaca gggacacgtt cgttgaatgc tccatacatg cttgctcacg
     5821 aaaatgatat tgcggcatat atcaaagaaa caaatcatca tgtacgctac cgtgtcactc
     5881 ctcattttga ggggaatgaa ctggtagcca ggggagtgca attggaagca gaaagcattg
     5941 aagacaaaaa aattgaattc aatgtcttta tctacaatgt tcaagatggc tacacgatca
     6001 attatgagac aggacaagca acaaaaacaa aaaattagta gcagctattc gaatttttta
     6061 tgaatattat gaaaaaattg tgtaaaaaca tacattcaaa gaaatatagc gtatacttta
     6121 tgaaaagagt acttttgata aagctacaga aaaaaggaaa aaaacataca aatagtgttt
     6181 cttaatttat tcggaagttt ggaatatttg tttgtagttt tccctttctt cgagtaaaat
     6241 aaggttgtag aaggtaagct ggaattacct tgcgggaaag aggtgtagaa tcatgggttt
     6301 tacagatgaa accgtacgtt ttggttttga cgatagtcgc aaaaaagaag tgagcgaaac
     6361 attagcgatt gtttatcggg ctttggaaga aaaaggctac aatccaatca atcagattgt
     6421 cggctacttg ctttctggag acccgtccta cattcctcgt tatcgtgatg ctcgaaattt
     6481 gattcgccgt cacgaacgcg atgagatcat ggaagtaatt gtcaaggact acctctcaaa
     6541 tcatggagtg aatttatgag agtaatgggc ttggatgtcg gttctaagac agtaggtgtt
     6601 gctatcagtg acccctttgg ctggacagct caaggagtag agatcattcg gatcaatgaa
     6661 gcagaagaag agtttggctt tgatcggttg aaggaattag tagcacaata tgaagtagac
     6721 cggtttgtag ttggattacc taaaaatatg aacaattcca tcggaccaag ggcagaagct
     6781 tccatgtcat acggagaaaa gatcaaagaa ctattctcct tgccagttga ttatcaagat
     6841 gaacgattaa ccactgttca ggcagaacgt atgctagtag agcaggcaaa tacttcaaga
     6901 gccaaacgaa aaaaagtcat cgataaatta gcagctgtga tgatcttaca aaattattta
     6961 gacggctcag gtaagcaatt attttaataa taaaagaaaa gaggtaatct tatgacagaa
     7021 cataaccatt cccacgacca tgaaggacac gaacacatca cattggtgga tgaccaagga
     7081 aatgaaacat tatatgaaat cctactaaca gttgacggac aagaagaatt tggacgtaac
     7141 tatgttcttt tatatcctgc tggtgtttca gaagatgaag atgtagaatt acaagcttat
     7201 gcatacatcg aaaacgaaga tggaacagaa ggcgagttag aacaaatcga aacagacgca
     7261 gaatgggaca tgattgaaga agtattcaat acattcatgg cggaagaaga agaatagact
     7321 ataaagctag gagaaacctt gatatgatag ggtttctcct ttttttattt tttactgtat
     7381 gccatattgt atgccatcta gtcaatattt aagtgttgta ctaatttttc aacagtcttt
     7441 tctttcgttt cttttgccaa gtgaccgtaa caatcgatcg tctgactaat ttttgtatgc
     7501 cgtaatctag tttgaacttc taccatagta gctccagcgt ccaagagcat tgaagcatga
     7561 gtgtgtcttg ctacatgcac accgagaaat ggaaccatag ctttttcaca aatagtaatc
     7621 aatcttgcat aaatagcgtg gtgtgtgatt agttctttat agataccaca aaaaatcata
     7681 gtcggatcag ttacaccaag tgtcagcata tatttccgct gattaatctg ccattttttt
     7741 aatatagaaa ttgttctttc atctagtgga agaaccccag cactacaatc agttttagga
     7801 tcactgattt tatagccatt tttggattgg cttagtgttt tattgatggt gaccgtattt
     7861 tcttgaaaat caatgtctga ccaattcaat gcaagggctt cactgatcct aaccccgcta
     7921 taaaagagaa agcgtagtaa cgttttatca aagtcgtttc gataagtttc aggtttgctg
     7981 tttaggtaat caaacaaggt gatcagttgt tctttactgt aaactttgac cttttcttta
     8041 tgcttagtgg tggttctttt aggcattaat acgttgtcta gtggattagt ctcaataatc
     8101 ccaagattga tagcgtagct aaaaatacgc ttagaagttg aatgaagtac cttgtattgg
     8161 tcagtagttt cagcccagct atttacaatt tgttgggctt tttttaccgt gacttcattc
     8221 acacgaagat ttccatattt tggaagaatc cactttctaa acttgatttc agttttcatg
     8281 aaggtggctt ctcggacagt ggttttatat gtttctagcc acaagtcata aacttcttta
     8341 aacgtttcgt tgttcgcttt tttaataccg tttttatcgt agtccacttg caagcgtgac
     8401 aaggcaagct gggcttcttt ttttgttttg taacctcgtc tacgaacctt atcaggtttg
     8461 ccagtcattt tgttcattcc aagataggct tcatagagcc aaaatttacc ttttttagtt
     8521 tcatattgct tgaatgttgc cataatagtt ttccctccat agtgtagcaa gccgtggggg
     8581 cggttggagg aggcagttta ttattatttt gaatagtgtt tatttagctc agtaacaaga
     8641 gctgtgaact cttttagaag atcattttca taaaattctt gatactcttt tttctcatct
     8701 gtattttttt ctgaatcatt aattaatgag tcatatcgta aaagcgtatt tagaagagct
     8761 gcgagataag caattggggt actcccgtca ggaaattcaa ttcgagatcc tgttttaaga
     8821 tattttattg cgtttgttaa ataaatcttc tgtgagacag tcaggttatc ttcttcgatg
     8881 ttattgatag cattagaaag attagaatca attatagagt tatttttttc aatagttttt
     8941 ttcaattgct tttcaagcac atttcgctta atttcgggca tcatatgcat atctcttaat
     9001 gtataagatc cgtttaatag ataggtagtg gatacatcta atacctttga aagttgttta
     9061 agcctggtgt tattgggaag attatagcca ttttcccaat tactaacggc acctttactg
     9121 gccggtgggg ataatttacc tccaagttgt tccattgaga gtcccttttc aattctagcc
     9181 agtctaattc tttgtcctgt ctccactgct tcggggtatt tttttctagc cacattttca
     9241 ccaccattca tatattttct ctactatatc acgatttaaa tatgtgtgaa acataaaagt
     9301 tcaaaaaagt atgttaaaat tcttgcaaat gaaaaggtat gatggtatat tgagtttata
     9361 agaggtatag aaatgtattc aagagcctct tgcatttttt taaaatcttt gtatagaaat
     9421 gtatgctaac gaggggggat aaatatggaa ttaaaaattg acaaactcac gcttgatgat
     9481 ccagctttta aaattattga cgatcgagtt gttcaacaag tagagcaaac tcaaggaacg
     9541 ttactttcat tattagaaaa aaagtaccag tttccaaaat atatgaacaa ggcacaagct
     9601 gctaagtata tgaacgtttc atacaatact atgatgcaga aatatgttcc taacggttta
     9661 aagttgatta tagttgatgg agtcgtaaga atttcacaag atgaatgtga tcgctttatg
     9721 gaagaatacc aaaaataaac gcaagccgtg ggggcaaagg gattaatagg aggaaataat
     9781 aatggaatcg acacaaaata aagcaatcga aaaagtatta tcaacaattc ttacagaaga
     9841 cacagcggca gaattagcaa acttggaagg caaagcgcta gaagaaacgt ttgaatgttt
     9901 gtacgaacaa atggactatc aaaaattgct accacaagaa ccaacggtaa gcggagtatt
     9961 gcgtggcttg aacgatttag tacaagcaga gtttaaagaa cgactttcta ttgaagagta
    10021 tcaagaaatt ttgtatcagc aagttgagtt gttagcgagc ttgctagggc ttgaactgga
    10081 ggacgttgaa tgaaaactga aatttggaac ggacacatta taagatttgt cgatattaat
    10141 gatgaatggt gggcagtggc gaaagatgtt gcggaagcgt taggtcttaa acaggttact
    10201 agagccattc actctttgcc taaagatggg gttactacta gtaaggtcat cgattcattg
    10261 ggcagaacac aagatgtaaa catcatcaac gaaaaaaaat atttaccgca tggcattcaa
    10321 aagccgtaaa aaagaagccg aggcatttca agactggatt ttcgacatca tcaaagaact
    10381 acgccagtcc acaggacttg aaggctttca agtattccgc atgctagaca aagaacacca
    10441 aaaagaagca atgactaaat taagccacgc tattactgaa ccaaaaccag tagactacat
    10501 taaagcgaat gtgatagcca ataaagctgt atcaactatt tatggtcatt ccaaaatggt
    10561 aaaaaagaaa gatatgactc ctgaaatgtt ggttgatcgt gaacctattt tagatgagac
    10621 agtagaatta atgactgtta aagaaaaata tgggttgcag ttttcagtga gtgagaaaat
    10681 ttataatcgt tctgctgaat tgcagacaac ttaggagcat taacatgatc gagaacaaag
    10741 aaaaagcctt atccataaca gtcttggcgg acaggataaa ggcaacttaa ttgggataaa
    10801 acccgatatt ctttgttccg attataccat attaaaaaat cggagggaac cattatgaaa
    10861 cgaaacttaa ttataggaag cattttaatc ggcggaattt tcggcttatt accattaatt
    10921 gcaaaaatag ctatctttgc aggattagcg ttatatctct tcactgctta tgacgagtac
    10981 gactatcaat taagaactgg agggcataag tagatgtaca aaacaaaact attaaaccaa
    11041 ctcgatagcc ttgaattaga ggaaattaat caagggatcg ctgaactaga aaataatatc
    11101 gggaaaacct actttggaaa ttcattcaat gaaaaactaa cagttttgta tgtactcaaa
    11161 aaacatgcag aacacaaaat aatttgtcga gaaatcaacg agctaaaaaa tcagatttta
    11221 accgcatggt taaacattac tgatatgcaa gaagcaaggg tgaaaacatt taatacatgg
    11281 gtaaagtatc agaatcaatt aaaaggtgct gaatttgttc gggatgggtt gaaatacgaa
    11341 cttgagcagt tgaagctcat ggaggtgtct gaatgattta taccagtatt ttgaaaaagg
    11401 aggagaggtt tattggcaaa taattatcaa gcagcaacgg aattacaaaa gtcgggatgg
    11461 tcaatttatc ccttatctcc tggaactaaa attccaataa aaggctctag tggttggaat
    11521 gatgcaacta acgattctaa tcaaattaat gaatggtgga cggaagatgc aactagaaat
    11581 attgggttaa tgcttgaaga tcatcaaatg gtggtagttg acctagatca acattctgaa
    11641 acacgaaacg gcgttgagaa ttataaaaaa cttgcttcaa tgtatgatcc atttccttct
    11701 acttatacgg aaatcacacc aggtaaagga atccatttct tctttaaaat tcctgatggt
    11761 gtaattatcc aacaagaaac aggtgcattt gctgatatct tcgggcgtga tgataaaggt
    11821 aaaagtttaa gcgggattga tattatcact aaaggagttc caatagcgcc aactatgatt
    11881 atttcaagaa gtgttgggag agcatatgaa cctattgaaa acgtatctct aaataatatt
    11941 gcaccagctc ccgattggct agtgaaccta ctgctaaaaa aaccgcaaga aataaagact
    12001 caataccgac ccgtagcaaa aacagggaca gcaaaaaaat tagatatgat tgttcaaggc
    12061 gctggcgaag gtagtagaaa cgatcattta actaagttat gcggttggtt actatggcac
    12121 ggtgtagata atcaaacttt agcagaattg atttatacag caaatgcgta caatgaacca
    12181 ccgctaaaag ataaagaagt gaatcaaatt attcgatcga tgattaaaaa agatttgagg
    12241 gggaaagctc atggctcata agcataaatt agatagtatt ttagactttc ccgaagccag
    12301 cgagcgtgaa gacaatatca ttgaattgaa aacctggatg tcacgattaa gatgcaataa
    12361 agacgatcag attaaaagta acagtgttgt gaatgcagaa ttgattttaa ccaatgatag
    12421 caatttagct ggaacaattg cttataacga atttagcgga tacattcacc tattaaaaga
    12481 ttctccttgg atcaatcgta ccgctggaga atgggaagac agttttgagg atgccttaac
    12541 ggcatatatt gaagaaaact acaatgtagt ttttgatgac aacaaaatac acaaagcagt
    12601 agtaaatgtg gctagaaaga atgttttcaa cccagtaaaa gaacgtatcg aaaaagttaa
    12661 atgggatcaa cagcctagac tggaaacact gtttattgac cttttagggg tagacgacaa
    12721 cctttacaca cgagaagtaa caaaacgttg gatagtcgga tcagtcgcac gtatctataa
    12781 accaggaata aagtttgaga tcgttccagt tttggatgga ccgcaaggga ttggtaagag
    12841 taccgttcca gcattacttt acacggatga ttttttcacc gattcattag attcgctagg
    12901 tgaaaagaaa gatgattata tgcagcttca aggcaatgta attattgaac ttggagaatt
    12961 atcttcaatg aacaaaacta aaattgaaca gataaaaaac ttcatcagtg ccaagatcga
    13021 taaaattaga cctccttatg gtcggaatgt catagcttgg gcaaggcaat gtgtatttat
    13081 tggaacgtcg aatgatgggc agtatttgaa agatgataca ggaaaccgcc gtttttatcc
    13141 tttgccttgt aagaataagc caaagatgaa tccgtttaaa acaaaggatg aatattttct
    13201 tcaagtgtta gctgaagcaa aggtgctttt tgataaagga caacgaatct attttagtcc
    13261 cgatgaagat aaagaagtct tagaaattgc aaaagactat caagaggatg ccaagatgga
    13321 gaatccaatt aaagaagcaa tcactaaata tttggaaatg gaaatcccgt ataaatggga
    13381 agaagctcca gcgtgggcga gacgttctta ttatcagaac tatcctgaca gtaaatgtaa
    13441 tgagaaaaat ttacagcatt tctataatgg agctactatg caacaagagt tttatttagt
    13501 tgatagcgta ctcaccgctg atattttgga agctgttttt gataaacaag caaaggattt
    13561 attgaatggt cgatcagatg cagaaactaa aaagattgcc ttgattatta gcagtattcc
    13621 tggttgggaa cggaagcaac tgccaaaaag aaataaacga cgaggatttt acaataagtc
    13681 aaatgcctat gaaaataaaa atcgagctgg aaagtacaaa aagtaacggg tagtaacggg
    13741 tcagtaacac ctagcccgtt actagtttac ccttactgcc ccaagggata cagcaatttg
    13801 gtaacggggt aacaccttat ttccttaaaa cttttgaaat agttagctaa tatgctgggg
    13861 aataatgtta ctggatttta cccgttacct gttaccaaaa cgctagaacc cttgtccccg
    13921 ttgggattga gtggtaacgg gtacctgtta cgtaggtgtt accacgtgtt acgaatcaag
    13981 gaggaaagta aatgctcaat attatttcaa caaacaaagc accgaacttt caatatacag
    14041 atgaaatgga tcggtttcta atgaacacgc tggcatttag tgtgggattg gtaacagaag
    14101 attattccac atttgatcca gaagtattaa agataatgga agaagaacct gattggctgc
    14161 aggagtcagt agcgtggtgt caatcgctag tagtgggatc attggtagat agtggcaact
    14221 atgatgatac aggtgagcta atggatgaat ttaattgtct gcttaaccta tatgatcgag
    14281 cgagacaacg agagcttaca tcgaatgagg ataatttgtt tttaaacatc catgataagt
    14341 tcttggcttt gctactaaca gatgatgaat tgataactaa tttattggag gtagaataat
    14401 tatgcatatg gaaagtatta gatacttcga tggtgagaaa cacattgagt attcaaacac
    14461 tcaagaagat gtagacttta ttagttttga ggaagataaa atcactaaag ttaattttaa
    14521 agacggaacg tgcttgaaga tagtttcgcc ttatattgaa tacaaatcaa aatgggaaca
    14581 agatgattct gacgaacgag atcgctttgg ttggtaatct atggcagtta aaaaacaatg
    14641 taatcatgct ggatgtaaaa cattgattga ttataggcag aagtattgtg ggaagcataa
    14701 agcaaagcaa acggcaatta agcgtgaaga aagaaagcaa agcgaaggta aatattttca
    14761 attctatcaa agtagaacat ggcgaaaggc ttcatacttg tatcggttaa atcatccagt
    14821 atgtgaggat tgcctagaag aaggcttgat aaggaaagct gatgttgtag atcacaaaat
    14881 tgaactaaaa gatgattgga atagacggtt agatgaaact aattttcgtt ctttatgcca
    14941 tgcacaccat aattccaaga cagccaatga aagacaaaga cgagaaaaga gtaccctttg
    15001 agttaagggg gcatagtgtg aaacttactg acaatcgatg cctactcatt ttggtacaaa
    15061 taaccgttgt gaaaaggcat aagggtaatt acaaataacg attacacttg taatcacaag
    15121 tgaaaaagta tataatgaaa gtaggagatt tattgaaaaa tacaattgtt attactgata
    15181 aaacagggaa acagcgcacc attgagttac ctcattttgg tcaagtgacc attcaaatgc
    15241 agaacggaaa aatcatttat atagataaat tagataaaga aaaattctga tcgaaaaacg
    15301 aaggaatgtg agcttaattg cttacgttcc tttttctttt gtctgaaagg aggacaacat
    15361 gggacaccct aaaatattac acgatacaaa aggcaatata tcaagtgaag aaaaagcaat
    15421 ccgtgtggac gctagagaag aattgttcaa gcagcaacca ttaataaata tcacgccccc
    15481 tgactggatg gcagcgagcg ctagaagtga atggaatcgt atcgtaccaa cattaaaaaa
    15541 agattatcca ttgagtgaag cagattatgg ttcattggta gcctattgtt tagcctttgc
    15601 tcgaatgaaa acagccgaag ccgagataag aaaatcaggt acgtttatca catgcgaaaa
    15661 cggagtaaag aaagccaatc cagcggttcg ggttcaatca caagctatga gtgatttgaa
    15721 aaaacaagcc acctcactag gtatgacctt agaatcacga tcaaaactag ctttgaacaa
    15781 ggttaaaaat gatgaacccg aagacccgtt caaagagttg atgaattcat gaatgattac
    15841 attgagaaag tcctatcagg taagctgatt gcacccaaga aaattatcca agcgtgtgag
    15901 cgtcatataa gcgatttgga gcgttctaag tcagatagct tcccttatgt gttcgatgaa
    15961 gaacaagcca ccaaagcgat taaattcatt gagttgctac catctacgga cggtaaagca
    16021 atcaagatgt taggatttca aaaattcatt ctaggcagtc tttatggctg gcgtactaaa
    16081 gaagggaatt acaggcgatt caatcgagcg tttaccagta tgagccgtaa gaatgggaaa
    16141 acgtatatcg caagtggcat ggctgccaat gcgttgatta tggaacaaga accagcagaa
    16201 gcaaggcaag tattgtttgt aagtaacgct ttgaaacaag ctaaattggg ctatgatatg
    16261 ctgtctaatt cacttagaaa cgtggtcaag tctagtaagt ttttaagacc acaactgaaa
    16321 attatgaact ctaagattca gcacttgccc tctaattcgt tcgctatggc actggctagt
    16381 gaaaccagca cgctagatgg gtttgcacca acaaccgcaa ttcttgacga gtggcacgaa
    16441 gcaaaaactc gtaaaacgta caacgtcatt aagtcaggaa tgacccaaca aaagaacggc
    16501 ttattgtgtg ttattagtac cgctgggctt gatttaaacg ttcctatgta cgaggaatac
    16561 ttattgttag atcgtgtgct aaaaggcgaa gaacaagccg acaggtactt tatagcgata
    16621 tgggaattgg acgatcccga agaaattcac gatcaagaga aatggatcaa agccaatccg
    16681 atttttgaaa gtgaagaaat caaaaaagta atgattccaa ccattcaaga tgatgtgaac
    16741 cttgctttga aacaaaataa ccttaattct gtattggtga aaaacttcaa tttatggaga
    16801 caagcgagtg aggacagcta tatgattgct gaagactggc aggcaaccga agtagaacca
    16861 caagatatta caggcaagcc cgtttatatc ggagtggatt tatctaaaac agatgattta
    16921 actagcgttt catggatcgt accgctagat aacggcgaac tttattgtga ttcacatagc
    16981 tttgtagcca ccaaatatgg gcttcaagac aaagaaaagc gcgacggttt gccttatcga
    17041 gaactagaaa aagctggtga gtgttccatt actcaattag aaagtggaat cgtggactat
    17101 gaccaagtat ttcagtttat tcaagatttg attcaagaaa atgatttgga atgtatgggg
    17161 atttgttatg acccgtataa cgctaattcg cttatcagta aagctgaaaa agccaactac
    17221 ccaatgttag aagtaagaca aggaacgatt actctaaacg ttccgacccg aacttttaga
    17281 gaacaagttt atgaaggcaa cgttattcac aataaaaata cgattctcac ccatgcagtg
    17341 aacaacgcta ttttaaaaac ggataacaac ggcattcaga ttaataaatc aaagaacagt
    17401 aacaaaattg atccaatagc tgcattaatc aatgcctatg tgtttgcaat ggattacttc
    17461 accacaacgg aaggagcgaa agcagacaat gaattttata caagtgaaga attttctttc
    17521 taattacatt cataccgttc ttttacttct tggattggtg tgtgtgttgg ttgctatcac
    17581 cttactaaca aatgtctact atggcttgtt agcgctgggc atagtgctta ttggggtagc
    17641 ggtcatgcta aatacagaac agaagggagg ttaaaagatg gcatttttta aagcgagaca
    17701 aaatacaacg ggagatcctt tcctacaaca tgtggtatca atccaatcgg atgattacac
    17761 caccagcttt acaagcgttc gtgcattaag aaatagtgat gtgtttgcag ccgttcgaat
    17821 tattgctagt gatattgctt caagtccgat tcaattggtc aaaaacaata tgccacaagc
    17881 tgataatgaa ctggtgaagt tactaaacga gaaacctaat tcagaaatgg atggttggca
    17941 tttaaagttt gctttggcgg tcaatatgtt gctaaacggt aatagctttg cagagattaa
    18001 acgcaacggt gaaaaggtag aagaaattca cttattacct aactcaagtg taacggttac
    18061 tcaattagat aatggtacgt tgtcttatca gattggtgat aaaaaaagac gtgtgaagtc
    18121 tagcgatatt ttgcacttca aatatttcac ccaagatggt ttaacaggat taccaccact
    18181 ttatgcgtta cgtgatgaac tgaaaattca acaagctggc aaccgcacat tgcacaattt
    18241 ctttactcgt ggtgtcagtg ggtcaggtat tttgaaggtt cataagtcag atttagacgg
    18301 atcagcaaag aacgcaattc gtgaaaagtt tgaagaagcc aacggttcaa gtagtggaga
    18361 taatgctctt agaacgatca ttcttgatga aacaatggac tacaaaacat tagaaataaa
    18421 caccgatgtt ttgaagctta tcaattccaa tgattggaac acaaaacaaa ttgccaaggc
    18481 gtttggtgta ccgattgagc gtttaggcgt tgaaaatgaa cattctagca ccgttcaaag
    18541 taatctccag tatatccaaa gtacgctgat tcattacttt aatgtgtttg tgagtgaatt
    18601 agatacgaaa cttgaaacca atatccgctt taattccgat cagttactag agactgatcc
    18661 tgaaacaaaa gtaaaaaatg tattggatca ggtcaaaggg tcacttctca cgattaatga
    18721 ggggcggtcg aaaatggggc taccccccat ggatggtggc gatcgtttac tagcaagttt
    18781 gaactttacg tatttagata cgttggagaa atatcaatta aaagaacagg aaggagttac
    18841 accagttgaa taatgaagaa gaaaaggaaa aacggctgac agaagaagct gagctaacag
    18901 ccgattctcc caaagtagag aaagaaaatg aaaaacaacc aacagacggc aaaactattt
    18961 caggctatgc gttgaaattc gggcaaccgt caaaagattt aggcggcttt gtagaagtga
    19021 ttacacccga agcattaaaa gaggtggatt tatcaaatgt gttcttatta tatggacacg
    19081 attattcaaa gccactagct agtgtcaaag caggtacgtt gaaattgaat gtcgatgata
    19141 caggattaca ttttgaagca gagctaaccg acacgaccta ttcaaatgat gtgtatgaga
    19201 atatctcaaa aggggttgtt gattctatgt cctttgggtt cgtgcttgga ttagattcat
    19261 tcgacaaaaa agaagatggc acaattgaac gatcaataga taaaatcaaa gcacttaatg
    19321 aaattagtgt cgtgaccgtt cccgcttatg attcatcaaa tgtccaagta aataagcgtt
    19381 cttacgaatc gtttatgagt aacaaccaag caaaacaaac aaacaatagc ttagaatcca
    19441 cttctaaagc acaaaaggag agtaataaca tggaaaaaac tttaatcgat aacgaaaaaa
    19501 cagaattgcg tgggtatgaa gaatatatcc gttcacaagg cgaagtgcgt gatggagtca
    19561 ctactgtaaa tgcagcggca gttgttcccg aagaagtaat cggtgaagtc tttgatttga
    19621 aacgttcaaa ttataactta gctcaatatg caacagtaaa aacagtatca aatggacaag
    19681 gtaaatatcc agtagcaact aaccaacaag cagtgttagc aacaaaagct gaacttgctg
    19741 aaattggtga tattgacgct gaaatgttta cttcagttga ttataaagta gaaactcgtg
    19801 ctggtaagat tgccttatca aatgaggttg tggaagattc agcagtgaat attgtacaag
    19861 aggtcaaaga tcagttagcg aaattggtag aaaacactga caataagcat atcatggatt
    19921 tattaaaaac attcactaag aaaacggctg ctacgttgga cgatttgaaa caactataca
    19981 atgtggcatt agacccagca ttaaataaaa tggtaattct aaaccaaagc ggatattacc
    20041 acctagatac attgaaagat tcagatggac gttacatttt acaacccgat gtgacagcac
    20101 ctagtggtaa atcattattc ggtatgccag tagtattgat tgcagataca ttgtttgcca
    20161 atcctaaagc tggaacattc cctatgatta tgggggatat tgcacaatct atctttgttg
    20221 ctcgtagaaa tcaagtaacg actcaatggg aaaaattcga ttactactca caaggacttg
    20281 cagtgatcgt tcgcaacgat tataagaaaa ttgatcaaaa tgcttcagtg tatattgagt
    20341 ttacgccagt tgtaacacca aaagtataga aaaatattgg gcggcggttt atcccactgc
    20401 ctttttttca ttaggagaga taaaatggta attttggata gcataaaaaa aagtatgcgg
    20461 atcgatcaca ctattgatga tgactttatt caacaattga ttgatacagc aggcgaatat
    20521 ataaaaagtg ctattgatag tagcgcaacg gataaagata tggataatta tcagcaattt
    20581 gatttggcgg tgtcattact tactcaacat tggtatttga accgtcaaga agccagcagc
    20641 gaacggatac cagtaacggt acaagcatta gtacaacaaa tgagaggtgc ttattatgcc
    20701 aatcattaag aatgtaaatg aattgactga gagagtcaag tttaaaaaga caaaacgggt
    20761 aaaagatgaa gatgggcaga tggtggatag tgaagaaact gtatttgaat gttgggctaa
    20821 tgtgcgttca caaatgctaa aagacgtgct tgctagtgta ggtaccattc ttgaaggaac
    20881 gttgacgttt attattcgat acgatcaaga ttatgaacta acaaacgata tgaaagtagc
    20941 ttggaaaaac aagagttata aaattatttc aatcaatgaa ggaacggcgt ttaaagatta
    21001 tacaacgatc atagctaaac agatttctta gaatgattac acttgtaaac gttgtgggta
    21061 tttagtataa ttaaggtagt aaatgaaggg ttagctgcct gaattttgca agacttagct
    21121 agtcgaaatc tattgttgga ctgaaaattg taggtgtggt tgcaaacatg ctggactaag
    21181 aaagatgata gatgatgaac gttcaatttt aatacaggca tgctaggact gattggatgg
    21241 ttttggcaag ctgattgtgt agaaatcaaa gaacacgtct gttgtggacg tgtttttttt
    21301 gttataattc aaaagaggtg atttaatgga caaagcagag tttacaaatt tttattggaa
    21361 gtattatcta catttagaaa atgagtttat taataccaca gattttgtga tgctcgatga
    21421 gaataatttc aaaacattct ctattgaata tcaaaaacta ctactagcga ttggttcgga
    21481 gtgtgagatt attttcaaag aactttgcgg atttgatagc agtagcaata aaagcataac
    21541 tgattttaaa gctgttatta aatctagcga attaatgtgt ttggataatg gtgttagggt
    21601 tatgaattct ttaacattga cttcactaaa accttttgga gatgggtggc ctgaagatac
    21661 accagattgg tggaaaatat ataacagagt aaaacatggt cgttcttcaa attataagga
    21721 agcaaatttg gaaaatgtgt tgtatgcttt agcaagtcta tatttattag aggaatattt
    21781 gcataagaaa gtaatactag aaggtgaagt agactttata agtccagagt caaatctatt
    21841 tacgttatct tgggaaagaa aacattcgtc gatgcaaaaa gtgacgtttg aaaaagtgga
    21901 ttcaaattat gtagctccac tcattgattt taaagaacaa gaagaaattt aaaaaccccc
    21961 acggcttatt gaagtttttg tatgccatat tgtatgccat ttggtttaaa tcgcttgaaa
    22021 aatagtgata gataactcta attcatttag tcataaacgc tgttttgata acctttatag
    22081 ttctttgata tgggtttctc attcatggcg gaagaagaag aatagaacaa aagaatgaat
    22141 taataagata taaaaaaagg gattgtgaca aacgtcttgt cacaatccct ttttccaaat
    22201 aaacgatgtt caatcagctg cccctcggca ttcgctcgaa aaacggcgaa atatgaagaa
    22261 ctattttgcc atttgtttct ctaataatca ggtcaaaacg ttgacttcac agtctcttcc
    22321 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
    22381 aagctgtttt ttgaaattgt tccttgtgac tcggagctga acggcaggct cacgacctct
    22441 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gttcaaccaa acgaaaatta
    22501 tgaggagcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    22561 ctcttttcgt ttagcactaa atttccttaa aaagtagaaa agctttcgaa gcctcgtttg
    22621 tttcttttac tgtataataa aagaaaagag taacaatcgc taaaagatat aaacaagaga
    22681 aaggaaatac aagatgattg gtctgattta ttttgtggtg atcgttctgg cgaacacagt
    22741 tggcgctatt tccgggatgg gtggaggcgt gttgatcaaa ccgattcttg atcttatcgg
    22801 cgcccattct gttgcaggga tttcttttta ctcaacagtt gcggtattca cgatgtccat
    22861 tgtttcgact gtgcgacaag tatcaagcgg gaaatcactg aattggcaga ttgtcggttg
    22921 ggtatctagt ggagctgttg ttggaggagt agtcggcaac attgtattcg aagttttttt
    22981 acagttattt gaaaacgaga agcatgtgca gctgatccaa atttttttga cggtattgac
    23041 cttggttttt gcgttcttct acaccaagca tcatcagcca aaatttcatt tgacgtcatg
    23101 gacttggtac cttatatgtg ggggggttct gggcttttta gctagttttt taggtattgg
    23161 tggtggacca gtcaatgttt ctttgttgat gctgatgttt gcattgccaa taaaagaagc
    23221 aacactatat tctctatcta cgattttctt ttctcaactg gcaaaattgg taacgattgc
    23281 tttaacaagt agctttatgc gctttgattt atctatgttg ttttatgtta tccccgctgc
    23341 tgttattgga ggtttgtggg gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac
    23401 ctttattttt caagtaattg tcctcgtggt tcttttgatc aatctttaca atgctttcgt
    23461 catcttacaa acattaggag aatctagact aacgcaagtc aaaatcactg aaacatattg
    23521 ttctaaatga aaaaaggagt atactgtgat aaaaaaattc atttaaaaaa ataaaaattg
    23581 gctggtgatt tggatgttgc gggaaatgcg tttaggagat gcagaagcac tacgagaaat
    23641 caatgcggaa caattaggat atgatgtacc attaaccgtt acggtgagac aaatgaagaa
    23701 actgttgaag gagccagaaa aaaatttttt cttggtctat gaagatccta cttctaaaaa
    23761 agcagctggc tatgtacaag cggagtccta tgaatcgatt ttcagcgatc cgatgttcaa
    23821 tattatggca cttgctgtgt ctaaacatgc tgaaaaaaga ggaatcggaa aggccttgat
    23881 gactggcgta gaagaagcga aaagacgagg aatatcagca ataagactta attctgcaga
    23941 atacagagta gaagcacacc gattttatga acgcatcggt tatcattccg ataaaatgca
    24001 aaaaagattt ttgaaaatcc tgtaaatttt tatcaaaaaa tacctggtca tcaagccgat
    24061 acttgatgat caggtatttt ttttgactaa gaaagtaatt gcatcaagaa acctgagatg
    24121 atcaaaacga tggcaccacc aagccgattg cccatttgag caaaagcaat aagttccatc
    24181 cgattagctg cagataagac agctacattt cctgtaccac ccatcgagtt gttgcataag
    24241 ccagcagtaa tagttgattc gattggatat aaaccaaaca aacgaccgac aaatcctgaa
    24301 actactgaaa tcgcgataac acttgtcaaa cataagacaa caaattgcca tgtcaaagca
    24361 gaagccaata catttagatt caataacgca ataccgatac cggcaagaac agctgctgtc
    24421 aagtttttga caatcaagtt gttgaacatg atagctgcat cttcgtaata ttctggtaat
    24481 aggtttgcga ttttacagat aacaacgata atgatcatga atgcgtaagc atggacttta
    24541 ggaacaaaat agttacagat ttgtcctaag atgaagaaag tcaatgatac catcaaaccg
    24601 acacctaatt gtggaatgga aggttttaca tttctaggtt ttccatcgcc aaggtcgcct
    24661 ttttccatta atttaccatt accgttgaat tttggcattg attgtcccgc acgagcaact
    24721 aacgctgcac caatgatagc taggacattt gtcattgctg tcgcaggaat caggcgggaa
    24781 atgatagaag aagcgtccgt acctaaaaca tttgcataaa tcgttgataa aggtacagct
    24841 ccggctccaa caccaccagc cattgcgggc aatgagatat ataaaacgga atttgcaaaa
    24901 ccatttccga taagcatacc tacgagtcct acggcaaaaa aggagacgac cattgaaata
    24961 agtgcaacgg gaataaaacg aacagaagct ttcatcaata gattacggtt cataccaagg
    25021 atgctaccag taatcaaggc agcaatataa aagtctaaaa agcccatatt gctgacaaag
    25081 ttttctgtgg attttactac atactcaggt aagatcccaa atgtagcaat cgcggcagag
    25141 gcaaagatcg caaaaacgga gccaccacct aaataacttt tgacgatagg gatttttgtt
    25201 ccgataaaat gaaataaatt accgaaaatg accaaaacag cgattgggcc aatcatattc
    25261 gtcggtaatt ttcctaaagc cattgcaatg atcaggacaa ggaccatgat caaataaagg
    25321 ggcaaactta cgccggtaat ttttgttgac caaaaccctt cttttttctt ttcctcaact
    25381 gcagttgcgg ttgttgtttt ttgtgccatt tttacagatc ctttcttatt ctgtatcgta
    25441 tgaaaaaagc caaagagaag cggcaaaaga gcagtagttg gctggctgct gttttgccgc
    25501 agtcgctttt ttctttttag acgtcataca aagtttctgt atgttctttt actataacaa
    25561 tcttaatctt ctaaagattt atattcagga acccatttag cagattcaac agcttctttg
    25621 atatccgtga tttcttcacg attcaatttt tgtgcaacaa ctgattttgc gacagtttca
    25681 gcaatcgttt gagagaattc agtgattttt gctactggag gcaagatagc tgctcctggt
    25741 tttgttacat caacgattcc gcctaatgct cgactagctt gagaaatgat ttcagcattt
    25801 actcgtgtag ctgttgaagc aatcaaacca agccctaaac ctgggtacat caacgcatta
    25861 tttgcttgac cgatttggta agttacaccg ttatattcca catcagcagc agggatacct
    25921 gttccaacta aagcttttcc gtccgtccat tcaatcaaat cttttgcttt tgcttctgcc
    25981 aattttgttg gattagacaa aggcatgatg attggacgtg gggtatgagc cgccatttct
    26041 ttcacgattt cttcagtgaa tgctccaggt tgggtagaag taccgatcat gatcgttgga
    26101 tggattgctt ttacaactgc ttctaagttt gtcaattcat cagcattagt gaattctgag
    26161 cgtttacgag cgaatgggat ttgaccagga gtcaatcctt ttgtatcttc aaacaataaa
    26221 ccctgtttat ctacttgata gaaatgttgg cgtgcttctt cttcaggtac accttgacgg
    26281 atcatttcat ctaaaaggat gtttgcgata cctacaccag cagtaccagc accaaatgtc
    26341 aagatcgttt gatctttcaa ttgctcacct gatatattca atccacctaa tacaccagct
    26401 agtacaacga ttcctgtgcc ttggatatca tcattgaaag tagtgatttt atcttcgtat
    26461 ttttctaaga tcgttgcagc atttcctcgt ccaaaatctt cccaatgtaa taacaattct
    26521 gggaataatt cagtagctga ttgaacgaat tgatcaatga attcgtagta agtttcgcct
    26581 tctacacgag catgacgatt ccctaagtat aatggattat tcaataattc ttcgttatta
    26641 gtaccagcgt cgattgatac aggcaagact tgtgcaggat tgataccagc cgcagcagtg
    26701 tagaccatca atttaccgat agcgatgtca acaccgttca caccccagtc acccatacct
    26761 aagattcctt ctgcatctgt cacaacgatc aaacgaatgt cgcgtccatc tgctgcgttt
    26821 ttcaaacttg ctttgatatt ttctggttcg tcgattgaaa gaaatgcagc atcttgtggt
    26881 ttcaagaaaa tttcgttgta ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca
    26941 ggcatgaatt caactaaatg ttcgcccatc aatttataaa acaatgtccg gttcgtattg
    27001 aatagattca ttaagaagat acgtttttcc aaatcggtct gtttgcttaa gtattgaccg
    27061 taagcttgga cagattgttg ttccaatgtt tggacagtac ttggaagcat accagtgatt
    27121 ccgtattttg cccgttcttc ttttgtaaaa gctgttcctt tatttagaaa aggattattt
    27181 aataagttta gccctgtaag catgaatgat tcctcctaag ttttaataca aacgaagtat
    27241 aggagtcaga tgaggatata gtcaaaaaga gcaggtataa taaattttat ttatatgagt
    27301 aatagagtca aaagaaatca aaatagcggt atttgttctt tttatatcaa caatccttgc
    27361 ttgattacta tgcgtatctc ttatatgcta aagaaagaaa agtagaagaa taaaggatat
    27421 gttagataga taaatagaat ctctgaagaa taaatcacgt gatcgtgacg ataaaataca
    27481 cttacgtcag gtgataaaaa atacataagt agaaaactaa gaggaggaac agaaaatgaa
    27541 agaagttcaa ttgatcagaa aaagcgaact atcagaaggt ggttgcaatg catgcggcgt
    27601 agtagaagca acaagctata cattaaagct tggtgccaac aaagcgataa tctctgagtt
    27661 gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttattg gagaagatat
    27721 ttatgagatg ttttcagaaa tccgtcagtt aaaaaaaggg gaaaactgca tagaggttca
    27781 tcatgagagc ccgaatgtga gattcaaacg tggtgataat gagatgatct tcaataatca
    27841 tgtgtccgat cacaccgagc tatatggaat cgtgaatcaa atactaactg agctttttgg
    27901 attgggacct tatgcgttca aggaagaaaa cggtaatcca aaattaaacg aagaatggca
    27961 agaaacgatt gaaatacaaa gaaacaaccc gcacttattt caataggcat ccacagtatt
    28021 tcgtaaaagg gactttgaca cgacttttgt caaagtccca tcgtttttta tctatttgtg
    28081 ttctaaacat ttctttcaga gtagtaacaa ctggaatgac tgaaatgtaa acagattatt
    28141 tgcctgtatc aatacggaag agtaatataa agtgtgtaaa cgctatcata ttattcattc
    28201 aattttttat agagggaagg cattgaaatg gaaaaaacgt atcaagcagg tacaaatgaa
    28261 ggaatagtgg attttatcaa tatggaagat ttagaaatag ccgcttcaca agtgattcca
    28321 gctggtggtt atggttacat cagcagtggt gctggcgact tatttactta tcaagaaaat
    28381 gaacgggcat tcaatcatca gctgattatt cctcatgtat taaaagatgt agaattaccc
    28441 gatacaacta cacattttga caaagaaacg ctgactgcac caatcatcat gacaccagtt
    28501 gctgctcatg gcttagctca tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat
    28561 tttgggacga tctatacagc aagttcttac gcttcttgta cattagaaga gataagagaa
    28621 gcaggcgggg agaaagcacc gcaatggttt caattttata tgagcaaaga tgatgggatc
    28681 aatttggata ttcttgaagt ggccaagcgt aatggagcaa aagcaatcgt attgacagcg
    28741 gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa atgggtttac cttcccactg
    28801 ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc aaacgatgga tgctgtgtac
    28861 aaatcttcca aacagaagct tagtccaaaa gatgtagaat ttattgctgc tcattctgat
    28921 ttgccagtct atgtgaaagg tgtccagtca gaagaagatg tctatcgttc attagaatct
    28981 ggtgctggcg gaatctgggt atctaatcat ggtggccgtc aactagatgg tggtccagca
    29041 gcgtttgatt cgttgcaata tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt
    29101 gacagcggtg tacgacgcgg acagcatgtt ttcaaagcaa tcgcatcagg tgcagatctt
    29161 gttgctattg gcagacccgt tatttatgga ttgtctttag gaggaagtac aggagtccat
    29221 caagtgtttg atttctttaa aacagaattg gaaatggtca tgcaattagc tggaacacaa
    29281 acagtagaag atatcaaaaa aataaaattg cgtgagaatc gttttattta agaaacaaat
    29341 gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc tatttttata gttcaaggat
    29401 gtcactaata gaataatttg aaacgaaaaa ataaaattga aatatttttt catacttaat
    29461 aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt tttctctttt atacaaaaag
    29521 ggtcataatt tcaagtaagc ggatgaattc atgaggattt tttgcatact gtctctacta
    29581 tacttaggtc actaagaaaa aggagtgatt tattatgaaa aagaacatga ttgggacatt
    29641 attactttca acacttcttc taggcggctt ggcaacacca gcttttgctg agggtcaagc
    29701 gacttcaaaa ggagatatta cctttactga accaacaaat accgtggagc ctcttaaccc
    29761 tactgatcct tcaaaaccag tagagccagc agatccggaa aatccagcaa ctggccagac
    29821 aggtccttta acactggatg tagttcccga attgccattt ggtacacacg agattgaaag
    29881 cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat acaccttatc tccaagtatc
    29941 agatcgtcga ggtgtaggtg cagatggcca agcacaaggg tggaacgtga cagtttcggt
    30001 ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct gaattagatt ttggaacatc
    30061 cactgttaaa agtacgtcag ataacgaatc cactggacca actagtcaaa ctgtaactgg
    30121 gttgagtaaa gcaagtgcag cgacaccaat atttacagca gcaaaagatc aaggtctggg
    30181 aacatggctt tctgtttatg atccagcaaa tatcacactg aaagtgccga aagcagcagc
    30241 tggaacattc acggctgatt tgacttggaa cctcgtggct ggcccagtag catagagtat
    30301 gaaataaatt caacaaactg cggtgaaaat actataggtg aaggattttt taccgcagtt
    30361 tttgttgaaa ggatgtggtg gacaatgaat caaagtaaac gactgtttgt ttcgtttttt
    30421 tctattttaa gtctattttt catttttccg tcaatcagcc aagcaaagga ttcagcagga
    30481 gactttggaa tcaaaccggt atttccagag aaccaaatag ataaagcaat cggttatttt
    30541 gatttattag tggcaccgga acaaaatcag acactagaag taattatcag caattcttca
    30601 gatgaagaac gaacatttga ggtctctgtc aatccggcgg taacgagtga tggtggaacg
    30661 atcgattata gtcaaaaaaa cccaacgtta gatgagacat tgccgtttga tgtccgtgac
    30721 gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac atgcagaaac aactgtacct
    30781 atagaagtga aaatcccagc aaaaagcttt aagggacgtg tattagcggg tattcatgtt
    30841 tcacctaaag aagaagctga aacagaaaat gctacagaag gagcacaaat caaaaatcgt
    30901 attgcttata atctcgcagt cgttttacaa gaaagtcaag agacgataga accggatctg
    30961 aaacttttat caggagattt ggacgaggtc aacgcaaagc caactgttca gcttcgtttc
    31021 caaaatcctc agcctaggat tatcagcaat ttgatattta cgagcaagat cttttatgaa
    31081 aatcagcttt atattgaaaa tacgtctaat gcatttttag ttgcaccaaa ttcgaatttt
    31141 cacttgaatc ttgatttagc tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc
    31201 atagcaaaat caggcgacag taatgaatgg cgatttacac aaaactttac gatcaaaaaa
    31261 gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag tacaagaaca atctttccca
    31321 tggctttatg caggattggt tgctgcagtt ctagtgttat ggttgatttt gctgtttttg
    31381 tgggtccaca agaaaaagaa aaaaggagaa acaacatgat gaggtgattt cgttgaaaac
    31441 atcgatctta ttgggaggga ttattgcaag tttgctgttc ttcagctggc aagagccagt
    31501 ccaagcagat accaaaagtt caggtacgat tacattttat gatctaagtc caattccgca
    31561 tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca cagctaccga tttataaaga
    31621 atctaatcaa catggcgtat tcccggagac gaatgaacaa aagatccgtt ttctttattt
    31681 gatcattggt atagaattat ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa
    31741 ggaggatgaa catgttgcta caaaataaat tttcattttt tcgctccttc ttttttactt
    31801 cgctttttgt tttaagtata tttatctttt caccttcggc agaagctgtt ggagcaacgc
    31861 ctcctgccat ttcttatgat ccatacacgt atgaaaatat cgtacctcca aatggtggac
    31921 gtagtgcagt caatggcggg aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat
    31981 tgactgctac ttatttgaac gatggttcaa atgatccttg gaaaggtggc acggtccgtt
    32041 ttcattggga aagccatgtg aaactaacaa atgtacttga tttggtgaat atcactgcac
    32101 aaggatttgc atttgtggcg atcatcaatt tgccggaaca tgtatcaagc agtgatgtat
    32161 taaaagcaat tgactggaac aacgcctact tgtatattgc cggtgccccc atcaaggtta
    32221 ctagcggaag tttattatca gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg
    32281 atacaaatac tctattgaca gatattattg ctattattac gcgaggcgaa ttcaacgtgg
    32341 acgatatccc gcttcaattt gatgttacag ctaatgtcgc gaagatgaca gaaaacggag
    32401 ataaatatga tacgagtcca aacaaaattt tgaccaaggg taaattacaa ccttctatgt
    32461 ctaaaaaggc cgttttttct gttgattttt atgattcgaa taatattatc caagatcgta
    32521 tgttcaattc gattttagct ccagctgcag gtagatttta tcctaaatta actggcggaa
    32581 acttgaatcc aggagcatat gcattttctg caagtgcaga gataaatacg tggaatagtt
    32641 acatctcacc aagtgattca acaggaaaat ataatacatt tgctgatacc acagaatacg
    32701 tggatggaaa taaccagaac cttccaggct ctaccaatag tcggacattg acgatggggc
    32761 tgactgaagg acgttttgat cagttgccat ttagtcgctt tgatcgcgta gtcaactatt
    32821 ttacaggaaa aaacgttaca caaggagcaa tactgactca tactccagca agcacaagtg
    32881 gattgaatca gacagccaat atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt
    32941 ctcctgttgc tttaaaggta ttggagcagt acaagctaga cggaacggtt ttaccaacaa
    33001 acttaacagc cgatagtcag tggggaaagc tcctttcttc tgcatcgtac caagtcacgg
    33061 ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag
    33121 gaacccaaaa attagcgaca ggatggacag atcaactttt ccaaacgatc caatcaaata
    33181 acgggggtgt aaatacagct acttctacac tacctgcatt ggcttcaggg caatattatt
    33241 ttgattatcg cttggtggac gatgtactta ctaacatgta tccgtctttt gcgtacaaat
    33301 ggcagtctga acaagctgga atcaccactc ttcctcaaat aacagtggct aattttccat
    33361 ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca acctgagaca ggagatcccc
    33421 tgtctgctct atcaggagat acgattcaag aaaatgtgac attcacgtta acaaaatctg
    33481 gggatgcgat aaccgacaaa aaaatcacga tctcacttcc tgaaaatact acttatatca
    33541 atggttcctt aaaattaaat ggtacaacta tcgcagatac cggcattcaa caaggagttt
    33601 ctgtcccagc agatctattg agcaaaatag gagataccat tcatcttact tacaactatc
    33661 aattaaatac tgtagatact tctttacaat ccgtcagcat actgacaaag gcagctgtac
    33721 tttccagcaa tattacatta gcagatggtg ctaagcttcc taacccagtt gtccaaacat
    33781 ctgctaaaac aattcttgtc cccaaacaag aattaacatt agttaatgta ccagatgatt
    33841 ttacatttgg taacgatctc cccaaaccgt tgaagacaag ttattatgaa tcaaaaggcg
    33901 atttttcttt tgatgtgcgg gatactcggc ttccaagcac gtctccttgg caactgacag
    33961 gtacattgac ttctttattt aagaatgatc aaggtcaaga attaagcgga acaaaattat
    34021 acttcaatca ttctggatca aagcaattga ttcagcaagg tcaaaacacg ttgatttatg
    34081 agagtgatgg aacagctaaa ggagaggttc tcgttgactt tcccgatacc gatggtttac
    34141 tcttagaggt aaatagcagt acgaatgctc aacctggcgc aacgtatcaa ggcatggtca
    34201 catgggaact gacagctggg ccaaccagct aagtgattag aatttgtatg gatctgtgat
    34261 aactgtcaca gatcctcttt ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa
    34321 atgcttgaag gagtggactt ggaacttttc agtttgcttg aaaaaaatga acaacggcaa
    34381 atcaaacttt tccaactttt attagaggaa aaaggagagg tttccgttaa taacgtactt
    34441 acccgcttat cagttgatcg ggctaccttg aaagaagatc tgttttattt acaagctaac
    34501 ttggaaaata tcaaacaatt ggaaattgtt atccaagaag ggaatatttt attaatcaga
    34561 catggaaata tagcagcagt cgatgtttat tatcattgtt acttgcgcta ttctacaaaa
    34621 tatcagatac ttctttatct gttggaaaat ggctcatttg atcggcagaa acttgtcatg
    34681 gaacttaata tcagttcggc tactttgtct cgcagagtga aggaattaaa tgattcttta
    34741 aatgagtttc atctgaaaat aaaaagcggg cggctgaccg gtccagaaag tcaaattcgt
    34801 tatttttatt tccaattatt ttggtttgga cgtccctatc ttgaaaacaa aaaagaatac
    34861 agtgatttat caggaaacac ttttttagat ctgctgcaaa aagagtttag ttttgctatt
    34921 acagaagaag gacgattgaa atttgaaata tggctttcca ttatgaagaa gcgaatgcgc
    34981 tgtacgaaaa aatccaaacc agattatagc aatcttcctg ttggattgtt tcaaacgaca
    35041 caatttttat tggacctcca tacatttttg acccgttact tctttcaata tgcctttcct
    35101 tggaatcaaa atgaaacgtt ggttttttat cttttcatga tcagtaactt tacattagat
    35161 acacataact cgcatgtcca ggagtttttg ataagcgaaa aatacaaaga cgacgtagta
    35221 gctcaattga atcgaacgtt taaacaatcg atagaagaat atttctcttt ttatgttttt
    35281 ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc aaaatcacgc tcattttgcc
    35341 tattttagag gatggatgaa tatttttggc caagaaggat taaaacagcg gttaactgaa
    35401 gtagaagata aacaattcct ggttttatgt gaaagattgg tcagacaaac atttgctatt
    35461 gttgatttta ctgaagtaga cgaggaaaat gcccgctttg aattatttgc tcgctatgca
    35521 tctattttac ggacgctgat caagcagtta gaattacgtc tgcaaattgc ttatgatctt
    35581 ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca tccaggaaca attgctgaat
    35641 acgatcaaat atgatttagt cgaatacgag aaagaaaagc agtatgatat cattttgact
    35701 agccaattga aagagtaccc gtcacagaag tccgctaaag tttttgtatt cactagcaac
    35761 gaatataatt atgatttccc ctatttgaac caatttttaa aagaatgtta tttagaaaaa
    35821 ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat tggtgcttcc catgaaggca
    35881 tcacagctgc cctaaatcta agaaaacttc aaccgaaagc agaagtttta ctgatcgatg
    35941 aagatcataa agatggacta ggctatgttt caaatggaat caacctttat ttaaaaggaa
    36001 agatacgtag tttagctgaa gttgcccata acatgcggac tcttcaagaa agtggggcaa
    36061 aactgatcac cgaatggaga gtaactgaac ttgatccaga caaacatcag ctcatcctcg
    36121 catccaaaga aggaaaaaac gagaccataa cctatgataa attaatcgta gcgacaggtt
    36181 cctctcctgt caccttgtat aaacaaattg aagctgaaaa tgtctacacc tataaaaatc
    36241 tagtgcaatc taagcaagta cttgccgctt taaaagaagc aaaagaagta gtgatcttcg
    36301 gtgctggtta cattggattg gaattagcag atgctttgag aaataaagga tacattattc
    36361 atctagttga ttatatgccg aatgttttat caagatattt cgataaggat atgatcaaca
    36421 gttttcaaaa tcagctgcaa actaagcaaa taaattttta tccaaatgaa ttcttgatcg
    36481 attggaaaaa atcagaagag aaagtagtat ccgtacaact tctgtctcaa acaataaagg
    36541 cagatatggt cattttttct gcccaaactc gtcctaatac gacattgtta aaagaaaaag
    36601 tggctttgta tgaggatgag actgtgatag tcaatgaata tctgcagaca agtgatccgg
    36661 acatctatgc gattggagat atcgtcccag tcagttttga caaaaataaa cggcatctct
    36721 ttttaccttt agttactcga gctgtacata tggcaagagc agtagcgcta actttgagtg
    36781 gtcagccaac cgcttacgac ctcagacaaa agataacagc agcagtcatt atagactact
    36841 ttttaggaac tgtagggctg acagaagatg aagcgccatt cttggaacaa aataccggtt
    36901 cgtgttcagg agaatttgat ctatttccac aatacgacga agaaaacaaa acagtaaatg
    36961 caaagctgat ctaccatccg gatacattag aaataattgg cggacagctg ataagccaag
    37021 aattcttatt atcagatttg aacctgcttg cggatatcgt gaaacacaaa actacgattc
    37081 cacagctagc agttgaggat tttggatttc tgtctgaata cacctctcgt tttcactacc
    37141 tcaatgagtt agcttttaaa gtactagcta agagagcaaa tagcaataga gtagataaaa
    37201 gactgtgaga aatatcacgg tcttttattg tgtggggata taataaatat taatcaatcg
    37261 agaataaaaa attcatataa tgaaatagcg gaagaatttt ttgagcataa tattcgccaa
    37321 aaatttatca agtgataaaa ttgttgtata aaatgattat ttcaggagga ggagttaaat
    37381 gtttttagca ttaaatgaaa ttatgcattc aaagttacgt tatgcgttag ttgcaggggt
    37441 gatgtttttg atcgcctacc tggtattttt tttgactggg ttagcttatg gcttagctca
    37501 agataatcga acagcagtcg acaagtggga ggcagattcg attgtgcttt cgaaagatgc
    37561 aaattcaaat ctagggatgt ccatgattac taagaagata gcagaagaag tcgaaggtgg
    37621 gaaagtggcg tatcttgccc aaacacctgg agtcgtgact agtaaagaca gtacagaaga
    37681 aggaaaaatc aatgttagct tcttcggtat cgataaaaat caattcatca tgcccaatct
    37741 tgttgaagga aaagcttttg ataacgatga tgaggcagta ggcgatatta gtttgaaaga
    37801 agaatatggt ctagccatag gagatacagt taagttatct ggtagtgaca agacgtttaa
    37861 actaactggc tttacggatc atgccaaatt caatgtctca ccagttctct atacaaccat
    37921 caatgcttac caagaaatcc gcttcgaaaa agaagataca agtgaaaatg cacggattaa
    37981 cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa gatttagaac agatcaaaat
    38041 ttctaaattc atcaatgaat tacctggata taatgcgcaa gtattgactt ttggatttat
    38101 gatcggattt ttgatcgtta ttgcggctat tgtgatcggg attttcattt atgtactgac
    38161 gatgcaaaag attaatatct ttggtgtaat gaaagcacaa gggatcacag gaggatttat
    38221 tgcgcgttct gtagtggcac aaacattcat tttatcattt gttggtattc ttcttggtct
    38281 attaggaaca gtgggaacat cccttgttct acctgatgcg gttccattcc aaagcaattg
    38341 gctgtttttt ggcgtcatta gcttgttgat gttagtagta gctgtgttag gcgcactatt
    38401 ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca attggataag aaggaggaag
    38461 aacatgaaag caatagaatt taaagaagta gaaaaaagtt tccaagatgg cgaccaagta
    38521 gttaaagcat tgaaagaaac aaccatgagt atcgataaag gggaatttgt tgcaatcatc
    38581 ggtccttctg gttcaggtaa aagtactttt ttgactttgg caggcggatt gcagacgcct
    38641 agcagtggct ctgtatttat caatgatcaa gaattcagta atcaaaaaga aagcgaacgc
    38701 gttaaactac gttttaaaga aattggcttt attttgcaag cttccaatct ggttcctttt
    38761 ctgacagtaa aaaagcagtt attattagta gataagatta aacatgaaca tcgtcaaaaa
    38821 gaagcaaacg aattatttga gcagcttggc gtggataagt tgatcaacaa gtatccagaa
    38881 gaactatctg gtggagaacg gcaaagggtc gcaatcgctc gtgcattgta caatgatccg
    38941 tccattattc tagcagatga accaactgct agtttggatt cagaaaaggc acgagaagta
    39001 gtcgatatct tggcaaaaga atcaaaagac aaaaataaag caacgatcat ggttactcac
    39061 gatacacggc tgattgacaa atgcgataga atatttatga tggaagatgg tgtattgaaa
    39121 gaaaaagaat aaccaaaaag caggtggaaa aatggtccat cgatacaaca aagatacaca
    39181 gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc aagggatatc tcggcacttc
    39241 aacaagagaa atcgcacaaa aagttgggat cacccagccc aatctctatc attatttcgg
    39301 tgataaagaa aaactatata cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc
    39361 tttgagagaa atcgttcaaa caagcgaaac gggatttcaa gtaaccttga caaaaatggc
    39421 gcagtttttg attgagacac atctggtcga tttgtttctg atgcttcacg atcttgaaag
    39481 caatctttca aaggaaacaa gagatcatct tttctttcta tggaagaaaa attatcgtga
    39541 accttttgaa gaaatctttt cagcaaacca atctgtgcta agaaatggga tttcaaaaga
    39601 aatcgcagca aggcattttt tcttagtgtt agcaccttac atcactcggt cgaaaagacc
    39661 tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta tatttgaatg gtgtattagg
    39721 agaagcgtaa agctgttata tagggagaaa atagttaggt gaagaagcaa gatatgacga
    39781 agatcgttat atcttgcttc tttatatagg tagtttttca taaatacaaa tttgttattc
    39841 tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga agtgttttaa
    39901 tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt gggtagatga
    39961 atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa aaatcatttt
    40021 agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc ttacttaagc
    40081 tgattttaag ttttggccct tagtctggat ctacgaggtg agagtcaaga atgaaaaaaa
    40141 taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa atagatgttt
    40201 ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg gttacaaaga
    40261 caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga aaaaatgacc
    40321 gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac ggcggagagc
    40381 tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat gatggctata
    40441 ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa acaaaaccgg
    40501 aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat gatactgggg
    40561 cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca gccataaaag
    40621 taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact acagatgaat
    40681 tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat gtctttatca
    40741 gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg acatattatt
    40801 actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact gatctgcctc
    40861 tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat cttcaacctt
    40921 ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa ttagtaacag
    40981 acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact tattttcagt
    41041 aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg ccgggcttat
    41101 tatcgcagat gcaagctttt aaacaccgtt taaggaagag gtcgtgagcc tgccgttcaa
    41161 ctctgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga ctattgtgac
    41221 ttgtgaccga ggagttggca tgagaacacc gtttaagaaa aaggttgtga aatcaacgtt
    41281 ttgttctgag tattagagaa acaaacggaa aaatagttcc tcatattttg ccgtttgttg
    41341 agctaatacc gaggatcaga tgattgaaca ccgtttattc ggaacaaggg actgtgacat
    41401 gacttttgtc acagttccat tattttttta gtagcttcaa agctttttct gtattggcaa
    41461 aatgaagttt ggcatatttt tcaagcatag gcatgccgcc tttctccaat agttttgccg
    41521 ctacttggtc agatttcgtt ccagctcctg acggaagggt gattccagct tcagctgatg
    41581 ctttgtctaa ctcttccaaa aagctggcac gactgatgat agaagcagca gcaacagcta
    41641 aatgatattg ttctccttta gtaacgaagt acagtttttc ttgtaattga tttttttcta
    41701 aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg
    41761 gtttttctgg ctcgattttt tttagcagca aatggatcgc ttgattgtgc aaagcgactt
    41821 tcatccgaac ggcattgtat tttggttgga tctcattgta tttttctgga gataagacaa
    41881 gcagtttata ggggatggtt ttttttaaga tgtcagcaag ctggatgatt tggctatctt
    41941 ttaactcttt tgaatctcta acaccaagtt tacgtaattc tgcaagctga tcacgtttca
    42001 cataagcagc acatactgtt agcggaccga aatagctgcc atttccgacc tcatcactac
    42061 ctaaaacgga taattgactg atattttttg gcaaagaaga ggctgttttt gctggcttgg
    42121 cttgtttatc ctgtgcctcc ccccatttat cagcttctat tgctgcattc gtaccttgaa
    42181 acatcacttt tccggatgta taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc
    42241 tagtataagg aatctgttta tttaacaaat aagagcgata gtatgctttc atttgactaa
    42301 gttggtcgtt tgttgctttg ataatgacgt tgctcataaa attctcctcg tttttataca
    42361 taaaactata ccgcaactca cgacttttcg ttttctaaga gattgcgaga catagcattt
    42421 ttttaaagtt tatcatataa tagttaagta tgtgaaaaat atttgtggag aacggtgaaa
    42481 gaaccaactt catggtacac taaataaaac tttcatcaaa taggaggaaa tgattcgtat
    42541 ggcgcacgaa aaaacacgct acaaagcggt gattgccaac caaacatata ctattatcgg
    42601 acgggaaacc aagcatcata tggatatcgt aacgaagctg atcaatgaac agttagcaga
    42661 attgaaacaa ttatcaccac aaatggataa tgaacaagca gcaatcttga tggctgtcaa
    42721 tgcgctttcg gatcaattga agaaacaaga aagaatattg gagctggaag aagaaacagc
    42781 agaattaaag aaaaaaatga tcaaatttac agagcttgaa aatcgtgtga aacgcattga
    42841 agcaatcgag aacgaagctc gggaagtatt gaaagaaaat ggtcaagcag atcataccat
    42901 taaaaatcat gtggaagcac aacagatttt aaacgagaaa cgaaaaggac aaattaaaca
    42961 aaaatcttct aattaagatc cttttatcaa aataagaaaa atttaaaaaa gttagaagag
    43021 aaaggaacgt agcatgctga gtttgctgat tttattttta ttgctaattg cgtttttttc
    43081 tggagcacgt cgcgggtttg ctttgcaaat catattcgcg attggatatg tattgtcatt
    43141 tattgctgcc cagcattttt acaaaccatt agcaagtcat ttggaattat acattccata
    43201 cccagctgtc accccaacat caaagttagc attttttgat caggtatttg cctttcacct
    43261 agatgaagct ttttatgcag gaactgcttt tctgattatt ttgctaattg gctggctgct
    43321 tacccgattt gtgggggttt ttgtccatgg attgacgtat gtgccgatct tgagacaggt
    43381 agactggatt gcaggaggca tcctgtcact aatcatggct tacgttacta tatttttgat
    43441 tttgcagtta cttgcctttg ttccattaga tgtggtccaa aatcaattcg gcgagcatag
    43501 tttcgctcgt tttatcgttg agagaacgcc atttcttacc aacaagatgt atgagctttg
    43561 ggtaacgaat attttgaatt aaggaaaata gtgctggaaa atatttcgtt ttttgtacag
    43621 agtagtttat ttaagaaggc tgcgtagcgt atgtccggca cagctctttt ttcatagtga
    43681 aagataaggt gataaaatga ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa
    43741 acagatggtc agacaatttg tagtgactgc acaaggaaaa gaagaactgg cagaattggt
    43801 accagtgagt gagaaacaaa cgatcacaaa ctggcttcaa gaaactgagg atggattgaa
    43861 agtgcaacgt ctgcgaggcg ggatccctat acctaaaatt gaaaatatcc gtccccatat
    43921 gaaacggatt gagatcggag cagacttgaa tggagtggag cttgcacaag tttccagagt
    43981 attgtctacg acaagcgaat tgaatcgttt cattgatgat ctttctgaca gtgaaatcga
    44041 atttgcacgt ttgtatatgt gggcagatca gttagtcacg atccctgttt tgagccgccg
    44101 tttaaaggaa gcaatcgatg aagacggacg cgtgactgat gacgcttctc ctgagttaaa
    44161 gagcataaga cagaacattc ggcgaagtga acaagctgtt cgtgaacagt tagatgggat
    44221 cgtccgtggg aaaaatgcaa agtatttaag tgatgcgatc atcaccatgc gaaatgaccg
    44281 ctacgttatc ccagtcaaac aagaataccg cggtgtattt ggcggtgtag tccacgatca
    44341 aagtgcttca ggtcagactt tatttattga accaaaacaa gtggtggatt tgaataatcg
    44401 tttgcgtcaa taccaaatcg ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc
    44461 tgctgaactt gttccccatc gtcaagaaat catccacaat acttatgtga ttggtaaaat
    44521 ggatctgatg aatgcaaaag cacgatttgg taaagaagta aaagccatcg tgccgggaat
    44581 cagtgaagac aatcatgtcg tcttgaaaca agcaagacat ccactgatcg atcaggaaaa
    44641 agtagtttct aatgacatca caatcggaaa agactatcaa gccatcgtca ttacaggacc
    44701 taacacaggt gggaaaacga tcacgttgaa gactttaggt ctgttacaac tgatggggca
    44761 agcaggatta ccgattcctg caggtgaaga aagtcaaatc ggtatttttg aggaagtatt
    44821 tgcggatata ggagacgaac agtcgattga gcaaagtcta tcgacctttt cttctcatat
    44881 gacgaacact gtagatattt tatcaaaagt caatgaaaaa agtctggtat tatttgacga
    44941 gctcggtgca ggaacagatc cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga
    45001 tcttggtaaa aagtctgctt atgtgatggc aacaacgcat tatcctgaat tgaaagttta
    45061 tggatataat cgggcaaata cgattaatgc cagcatggag tttgatgtgg atacattaag
    45121 tccaacgtat cgtttgctga ttggcgttcc tggacgaagc aatgcgtttg aaatctctag
    45181 ccgattaggt cttgatacag aagttattga tgaagcgaag cagttgatga atgatgagag
    45241 ccaagattta aacgaaatga tcacagattt agagaatcgc cggaaaatgg cagagaccga
    45301 atatctggaa atgcgtcatt ttgtttctga ggcacaagaa cttcacgatg atttgaaaga
    45361 agcttacagc tatttctttg aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc
    45421 aaatgaagtc gtgtcagagg cggaagaaaa agcagaaaaa atcattgctg atattcgtaa
    45481 aatgcaacag cagattgggc aaggaaatgt gaaagagcat cagttgatcg atgcaaagac
    45541 ccaattagca aatctacatc aagaagaaac tttgaagaaa aataaagtat tgaagaaggc
    45601 gaaagaacaa aaaacgttga aaccgggaga cgaagtgttg gttacgactt acggccaaag
    45661 agggacgctt ttacgtaaaa acgggaatca gtggcaagta gaaatcggta tattaaaaat
    45721 gaatgtgtcc gaagacgagc tgacgcctgt tgctcctcaa aaagagccga cacaacgagt
    45781 tatccatgct gtacgatctg aatctagtag tcacgtgccg aatcaattgg accttcgagg
    45841 aaaacgatac gaagaagcgc taagtgaggt agatcaatat ttggattctg ccattctagc
    45901 tggttatcct caagtgacta ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat
    45961 tactgattat cttaaaaacc atcggagcgt caaaagcttt gagtttgcac cggcaaacca
    46021 aggcggaaat ggtgcgacaa ttgttaaatt taagtaaagc ttactaaagg taagcagatg
    46081 gttcgctatc caagtataga aatgctatac tagtcataga ttttaaaaat ggaggtcttt
    46141 gatatgtcac aagtaattac agataagaca tttaatgaag aaacagataa aggacttgta
    46201 ttgattgatt tttgggcaac ttggtgtggt ccatgtcgta tgcaagcacc gattttggat
    46261 cagttggaac aagaatatga tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa
    46321 aatccagaaa ctcctcaaca attcggtatc atgagcattc caacattaat gttgaaaaaa
    46381 gatggtcaag tagtagaaaa agctgttggt gttcattcaa aagaacaact acgtcaaatg
    46441 atcgatcaat atttataaaa acttacagca aaaataagct gagggtggga ctgaatccag
    46501 ttccgtcctc ggtttttttg cggttgagtt aaggttattc aaattaatat tttttgagta
    46561 taattacgtt gtcaggctgg attgtttttt tgacagacat gctataataa ctttaattga
    46621 atatgaaggg gagtaccaga tgaacgaacg aatcaaaaac aaattagctt tacttcctga
    46681 tcagcccggc tgttacttga tgaaagataa aaatggaacg atcatttatg tcggcaaagc
    46741 caaaatatta aaaaatcgtg tccgctcata ttttactggt agccataata cgaaaacgga
    46801 acggcttgtt agtgaaatcg aagactttga atacatcgtt acagaatcga atatcgaagc
    46861 attgctctta gagatcaatc tgataaagaa aaatgatcct aaatacaaca ttatgttaaa
    46921 ggatgataaa acctatcctt tccttaagat aacgaatgaa aaatatcccc gtttggtgat
    46981 tactcgtaag gtactaaaag acaaagcact gtattttgga ccatatcccg atgtcggtgc
    47041 ggcgaatgag acaaagaaga tcctcgatcg attatttcct ctgagaaaat gcaaacctag
    47101 tcagacgaag gagccttgtc tttactatca tttgggacaa tgcctgtgtc cttattattt
    47161 cgacgtagat ccgaaagtct atacgaaaat cgttgatgaa gtgaaaaact ttttaaacgg
    47221 aggacacgaa aaaatcgagt cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat
    47281 ggagtttgaa aaagctgcag aataccgaga ccagatccgt gccattgaga caatcatgac
    47341 tcgccaaaaa atgacaaata ctgatttact ggatcgagat gtatttggtt atgcgattga
    47401 taaaggatgg atgtgtgtcc aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga
    47461 tgtttcaatg tttcctttct ataacgaagc agaagaagat ttcttgactt acctcggtca
    47521 gttttatcag gaaaatgagc atttcattcc taaagaagtc ttgattcctg atacgatcga
    47581 caagcccagt gtagaagctt tgttagctac gaaagtgctg caaccaaaac gaggagaaaa
    47641 gaaaaaatta gttgaattgg cgagtaagaa tgcctcagtt gctttgaatg aacggtttga
    47701 tctgattgcc agaaaacagg aacggacgat cggagcagtt gaacgtttag gaaacgcgat
    47761 gaatatcccg accccaatac gtatcgagtc ttttgataac tctaatatca tggggacgaa
    47821 tcctgtggct gcgatggtcg tattcattga tggaaaaccg gcaagaaaag aatatcggaa
    47881 atataaaata aaaacagtca acggaccgga tgattatgca tcaatgagag aagtcatcta
    47941 tcgcagatat tcgcgagtga ttcgcgaagg gttgccttta cctgatttga tcttgatcga
    48001 tggaggaaaa ggacaagtag atgtggcaaa agatgtctta gaaaatcaat taggaatcga
    48061 tattcctatt gctggcatgg taaaaaacga taaacataaa acaaatgagt tgctattcgg
    48121 tacagatttg gctgtagtac cgcttgagcg aaattcacca gagtttttct tactccagcg
    48181 aatccaagat gaagtccatc gattcgcaat cactttccat cggcagctac gcagtaaaaa
    48241 cagttttgct tcccgattag atgaaattga agggttagga ccaaaacgaa aaaaaatgct
    48301 gcttaaagag ttcaaatcgt tgaaaaatat cacagcagcc acgacagaag aattacaaga
    48361 aatcggactg ccgaaaaatg tcgcacaaaa cgtatttgat aaattacatc aggaatgagg
    48421 aaagggcact ttacaaagaa agtaaagtgt cctatcattt tatgtacaac aaaaagagcg
    48481 tttacgtttt gttggtaaat aataaatcgc ttacgtctgg tgtctaagga tatatgtgat
    48541 ttcttgaaga aggagtgaag aaatgttaga agtcaaggat cttgttaaaa cttttgggaa
    48601 ctacacagct gtcgatcatg tctctttcca aatccccgat ggaaaaatca tggggctaat
    48661 tggccaaaac ggggctggga agacaacgac ttttcgtttg atcttggatt ttttgacaca
    48721 agaccaagga gaagtgttat ggaatggcca tcatcttggg gagaaagatt atgatgtcat
    48781 cggttatctt ccggaggaac ggggacttta tccgaaagta acgattcaag accaactgct
    48841 ttattttgca gaacttagag ggaaatcaag aaaagagatt gaaccaaaaa tcgacttttg
    48901 gatggaaaaa tttcaggtaa aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg
    48961 aaatcagcaa aaagtccagc tgatcgccac gttaatccat gaacctaaat tgatcatttt
    49021 ggatgagcca ttcagcgggt tagatccggt aaatgctgaa ttgctaaaag atggaatatt
    49081 ggaattgaaa aaacatggtt catgtgtcat tttttctagc cacaatatgg ataatgtaga
    49141 aaaaatctgc gatcatttga tcatgttgcg aaacggaaaa atggttttga atggaaaagt
    49201 acatgagatt cgtgaatcat ttggccgtac gaagttattc ttggaatctg gtttaagcca
    49261 acaggaagta gaagaaatcg aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt
    49321 agagattacc ttagaagatc ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt
    49381 cggctacatt cctatgttta accagcaacc gccaactttg gaagagattt tcaaattgaa
    49441 agcaggtgaa atcgatgcgt aagttttgga ttattaccaa agatgtctat ttaaaaaatg
    49501 ttaagtcgat ttctttcttg atcatgatat tagtcccttt tatcatgctg ggtatcattt
    49561 atgtagcagg aaactttgct caacaaaata gtgatgtcaa taaaatcggc gtattggctg
    49621 ataatgagca aatcagtcag caaatgagcc aagtcaaaac agatgatttt gagttcaaag
    49681 cagttgattc tgaagaaaaa gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt
    49741 tagtagtctc aactgagaat gataagataa atgggatgct ttatagcgaa aattcattag
    49801 gacaaagtac acaactactg atacaacaac aactgactgg cttgcaatcg atgcttcgag
    49861 cgagtcaatt aggcatttca ccagaagaag tagctagctt gagccaacaa gcaagtttga
    49921 ctcgacaaaa agtcaatttt gattcaaaag gaaaaatgac agtcggagaa gataactcga
    49981 cagtccaata cgtcgtaagt tatgtagcaa cgattgtctt gttcattatt attttgacgt
    50041 atgcgcagat cattgcacaa gagatcgctt ctgaaaaagg cacgcggatc atggaagtga
    50101 tcctgtccag tacgactgca caaaagcatt tttacggaaa attaacaggt gttttacttg
    50161 ttgcattaac gcaaatggca ttatacggta ttatttttgg tataggatac agtcaattca
    50221 aagatatgga aatggtcaaa aatatattag acggtatctc attggataac ttgattggac
    50281 cattcttgtg gtattctctt ctattcatga tctttggcat tttgattttt tctgttttag
    50341 cagctttatg tggctctttg gtcaacaaag cagaagatac agcaaaagca attttgccag
    50401 tgacttatct atctctaggc ggatacatgc ttggattgat actaggagct tcagatccaa
    50461 acaatatcgt tattcggatc acttcgtata ttccattctt atcttcatat attatgccga
    50521 ttcgcttagc aaatgagaca gtcggtatgg gtggagcatt gatttccttg ttggtacttg
    50581 tagccgtaac attcttattg atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg
    50641 tttatagcga aggcggatta tggagttcat tgaaacaatc gatttcaatt atgaggaatg
    50701 aacgaaaaaa agcataaaac aatcctaaaa tacagaagga agctggacat ctattgttcc
    50761 cagcttcttt ttgtatagac ctattttatt tattttacta tattggatat atattcattg
    50821 aaaatagtgt ataacaactt tataatattg agtataccaa tttttggagg cgttttaatg
    50881 gaccgttatt tagatttact ttcagaaaaa ttcccctcaa cggaggatgt catcacggaa
    50941 atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagtgat
    51001 atacatggcg aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa
    51061 gaaaagatca aagacctttt ttctgaacaa atgacggaaa cggatatcag ccaattcact
    51121 atttttacgg cttatccaga atatgcatta acgactgatt ggtacaaaga acaagataaa
    51181 tcgcagctga tccatcattt gatcgatctt ttacggttta cgacagtcaa atatacccgg
    51241 tcaaaagttc gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat
    51301 atcgatgacc gtatcaatgg aaaaaaagac tatgtaaaaa aaatcattga acaacttgtt
    51361 ttgatgaagg aagaagaaaa atttttaacg aaacttgcac agacgatcca aaaatctgtc
    51421 atcgatcatt tgcatatcgt tggagatatt tttgaccgag gaactcaagc agccaaagtg
    51481 atggatcgaa tcatggattt ttcttctgta gatatccaat ggggaaatca tgatattttg
    51541 tggattggtg cgtattgcgg ctctgaggca tgtttactga cacttttacg gattgcagca
    51601 cgctataatt atttatttga attagagaaa gaatatgggt tgaatttacg tccattgttt
    51661 tcttttgctc atcaaactta tcagaagaat ccagtatttg cgcctaaaaa cagtaaaaat
    51721 ctatccgaaa gagagcaaga agaattagaa aaaatccatc aggcactttc tatccttcaa
    51781 ttcaaatcag aacatcagtt attggatcgt cgacctgaat ttaaaatgga cgatcgtaaa
    51841 ttgctggaaa aaatcgacta tgaagcatca acgattgatt tagaaggtca gctctatgag
    51901 ttgaaaggaa cttgttttca aacgatccag ccagatgatc caaaaaaatt cttggaagag
    51961 gaacaaaaag tgattgatag tttgatgtat tcattccagc actctttgag aatgcaaaaa
    52021 catatgacgt ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtgctg
    52081 tttcatggct gtatacctgt agacgagaag ggacagctct tagcatttga attagaccaa
    52141 tcttatcgcg gaaaagaact gctgggcttt tttgaaaaac agataactga aagcgcaaaa
    52201 gatctgactg caaaagaaga tttagcaacc gatttgattt ggtatgcgtg gagtgggcca
    52261 ttttcgcctt tattcggcaa gagtaaaatg gcgacttttg aacgttattt tctcacagag
    52321 cctcatacgc acgtcgagaa aagcaatcct tattatcact tgcgagatga agagtggttc
    52381 agtgaaaaaa ttttagcaga attcgacaca aaagaagaag ggtcagcaat cattaatggg
    52441 cacacgccag taaaagtaaa aaaaggggag tcaccgatca aagcaaatgg aaaagtattt
    52501 gtcattgacg gaggtttatc caaagcttat caaaaaacga caggaattgc cggttattca
    52561 ctattatgca attcttatgg tttccaaatc gtgacacatt atccgttttt gcctattgaa
    52621 caacaatttg caaaaaaatc cgaccaaaca aatgtgaaaa aagtaatcga acagcaacta
    52681 ccacgcaaac tcaatcgtga tacaacaaaa ggtatggaac tgcagcagca gattacccag
    52741 ctcaaaaggc tgttggacta tcgaaaggac gattgagaag aattccagtg aacattgttc
    52801 catagactgg agactgagac aaaagtcgtg tcgcagtctc tttttccgga taaacggtgt
    52861 tcttctgcca actcctcggt cacaagtcac actattcaac aaacatgaaa agctgttttt
    52921 tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctctt ggtataaacg
    52981 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt gaaaagccat
    53041 tttcacaaaa tttttcttaa tactcgaaac taaacagctt tttcacaacc ccctcagaaa
    53101 gtctattaaa attcattcac aaagaaatcg aataagcgct gttcagatgg atgtacacgg
    53161 tgggtaagtt ccggatgcca ttgaacaccg aggattttat gcatatcatc tttgctttga
    53221 accgcttcaa cgagtccatc gttagaaaaa gcgatggctt ttaataaagg tgaaagatct
    53281 ttgactgcct gatgatggta agaattcaca aaatatggat cggggagaag gttgcttaac
    53341 aaactgtctg aaacaatctt gacttcgtga gtcgtaaatt gcggggcagt gggctgttgt
    53401 tcatgtttta ctgaccactt tggatataat gaaagatctt gataaagtgt accttccaaa
    53461 acgacattca atagttggag tccccggcag actgcaaaaa ttggttttct ttgtttcaat
    53521 gcttccaaga tcagtgcctg ttcaaattgg tctcgttgaa tatttgtttc cgttagcttt
    53581 ggatgaggat cttccatgta aagttgtgga ctgacatctt gaccacctgc aagaataagt
    53641 ttgtcgattt gtccaatcag ttgaggagct gaatctggag acatgactgg cagaataaga
    53701 ggcaatcctt gagcatctaa aactgcatcc acgaatcctt gcggtgtata gctcacttga
    53761 tttccttgga aagtatctgt tgctcgaatc agttgatttc cggcaatgcc gatgattggt
    53821 ttcatttgta ttccccctaa agtagactta ttgtaacaag gatttaaaaa aaattaaact
    53881 atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaaat
    53941 ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta
    54001 agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag
    54061 gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat
    54121 gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag
    54181 ctttggaaca aatccgaaaa gcagaagaag aaaatgaaaa gcgtgctcaa acactgcggc
    54241 agcagctgaa gatctatgca acagaaaaag agcaggaatt ggaaacgatc caaacaaact
    54301 atcgacaaca actaaaacag ttggtggaag aaaaagaaca atttgaaata caagaattag
    54361 aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagatc
    54421 aatatcaaaa gcatcagagt caagcgatcg gtgcgatcat tgaaagggtg aaagaaactt
    54481 atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg
    54541 attttacaag cagtacaagg aatccaaaac gttgaaatcc gggatctgta tcaaaataga
    54601 acaaacaacc agtgggtgga gaagtatttt ccagatacca cgattattga taaagaagca
    54661 aaattaagcg ctttaggtaa tcggttgaaa gacatccaag aagctatcca attcatacaa
    54721 caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa
    54781 gatttggaag ccaattattc cgaagaaaat ttcataaata aattgaaaga agtccttgat
    54841 ttaaaagaaa agtgggaaca actaactgaa gaacgtgatc gtttagaaga agaggaagaa
    54901 tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc
    54961 atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactgagt
    55021 aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg
    55081 tatgttgctt taaaaaaatc agttgctgtt atagaagaaa ttgctgggaa atacgggttc
    55141 aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aactgcaaga gaccaagcag
    55201 caattatcca ttgttcacga gcagcaaaag aagataacga cagcgttagg tcagtgcagt
    55261 ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgagaa
    55321 aaaataaaag aacggtttat ccatgcgact tatctgatcg ttttgcaagg ttgggtagat
    55381 gcagaagaaa aacagaattt gctgcatgtg ttaaacgaaa ccgtatcaga agaagatata
    55441 tacgtcagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg
    55501 aaaaaccatc cgcttgtcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa
    55561 tatgaagaag tagatccaac accttggatg acaccatttt atcttgtctt cttcgggatg
    55621 atggtcgcag atgttggtta tggtttgctg atgctcattg ggacaatact agctcaaaaa
    55681 ctacttgttc taccacgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcgata
    55741 ccatcgatca tctggggatt gatctacagt tcatttttcg gacagtcatt accaaaagaa
    55801 ctgtttggca tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt
    55861 ttgattttat ccgtgatttt cggtctgata cagattttgg ttggattatt tgtggcagca
    55921 aaggaaaata tcaggagaaa agattacttg agtgctgtca gtgatggatt tgcctggcaa
    55981 gggatcctaa ttgggatcgt tattgcgttg gttggtgcca tgttgataaa aaatacagca
    56041 tttgtttacc ttggcgctgg gctatcgatc attgctgcct tatgtatctt gatcgttccg
    56101 attatccaaa cgccgtctaa agtcaaaggt gctgccaaag gtgcgtataa tctttacgga
    56161 ctgaccggct atatcggtga tttagtcagt tatacacgtt tgatggcatt gggtatttca
    56221 ggcggaagta ttggagcggc attcaatatg ctagttgctt tcatgccgcc agctgctcga
    56281 ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg
    56341 ctaagtgcct atgtccatgg cgcacgattg cagtatgtcg aattttttgg gaagttttat
    56401 acaggaggcg gacgtgcctt tgatccgctg aaaacggcgg aaaaatacgt caacatcaat
    56461 cacaagaaaa aaaacaataa ataattggag gatttttata taatgatgga ttacttaatt
    56521 agtcaaaatg gtggaatggt atttgcggta ttagcgatgg caacagcgac gattttttca
    56581 gggatcggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc attgacaaca
    56641 agtcaacccg aaaaattcgg gcaggctctg attctgcaat tgcttccagg cacgcaaggt
    56701 ttgtatggat tcgttattgc ctttttgatt tttatcaact taggaaatga catgtcagtt
    56761 gtacaaggac tgaactttct aggtgcttct ctgccgattg cttttacagg cttgttttca
    56821 ggtattgccc aaggaaaagt cgcagcggca ggtattcaaa ttttagcgaa aaaacctgaa
    56881 catgctacaa aaggaatcat ttttgctgcg atggttgaaa catacgcgat tttaggtttc
    56941 gttatttcct tcctattagt gttgaatgcc taagcataaa ggaggaaaag cagatgaacg
    57001 ccattgaaaa aatcatctct cagatgaacg aagcagcaga gcaggaaaga gcggcgttag
    57061 aacaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caagttgaaa
    57121 cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact cgaaaaaaaa taccgtcagc
    57181 taaggaatcg ccagcaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt
    57241 taagacgatt gtttgcagat gcggtaactg aaatggaaaa ttgggatgaa tctgaacaaa
    57301 tccagtttat caaaaatgca ttgtattcat tacctttgac aggaaaagta gcattcattg
    57361 ctggagaaaa atcagcagcc tatctttctc agacgttgtt ggatgaatgg aacaatgaac
    57421 ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa
    57481 acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa
    57541 cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata
    57601 ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt
    57661 tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat
    57721 ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga
    57781 gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat
    57841 ctatacgatg cgttatactt tccataatct gaaggttttg acaaaagctg agatgacaaa
    57901 tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga
    57961 tgccattcat acgcaagttt caaatgaact tcctgaaagc attatggaca gtatacgaga
    58021 agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg
    58081 acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac
    58141 agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca
    58201 gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga
    58261 agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc tattagagac
    58321 agattatgct gattttctgc gccctgtcat ccgtgaggga aatattgatc tcgtaaagct
    58381 tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt
    58441 cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg
    58501 tttactggtt atcggcaaaa gaagccattt cgaatcagaa acaatcagaa agcgggtgag
    58561 acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca
    58621 aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg
    58681 aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga
    58741 gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca
    58801 atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag
    58861 taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca
    58921 taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca
    58981 tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg
    59041 cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta
    59101 ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa
    59161 tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag
    59221 ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg
    59281 gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac
    59341 ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat
    59401 tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga
    59461 tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg
    59521 cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag
    59581 cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa
    59641 aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga
    59701 cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg
    59761 aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta
    59821 tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca
    59881 tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag
    59941 aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg
    60001 aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa
    60061 tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc
    60121 gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt
    60181 cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc
    60241 atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag
    60301 aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc
    60361 gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg
    60421 atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga
    60481 cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg
    60541 acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat
    60601 tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag
    60661 aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg
    60721 atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa
    60781 aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa
    60841 atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat
    60901 cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga
    60961 ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta
    61021 atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc
    61081 gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt
    61141 cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat
    61201 gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg
    61261 gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac
    61321 gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt
    61381 gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag
    61441 gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg
    61501 tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa
    61561 ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc
    61621 cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag
    61681 agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga
    61741 actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc
    61801 tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct
    61861 gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa
    61921 ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg
    61981 atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca
    62041 gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact
    62101 cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa agataagcaa
    62161 gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa
    62221 atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg
    62281 gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta
    62341 gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg
    62401 acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc
    62461 gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt
    62521 caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg
    62581 acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag
    62641 cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta
    62701 tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc
    62761 cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac
    62821 ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca
    62881 ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga
    62941 acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga
    63001 tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta
    63061 tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca
    63121 tcctaaaatt ggcctttgtg atccaattga ttggtacggt tgctttatct tttgtatttg
    63181 tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt
    63241 tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt
    63301 atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc
    63361 gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa
    63421 ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg
    63481 gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg
    63541 tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc
    63601 tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat
    63661 tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta
    63721 cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt
    63781 tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa
    63841 cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta
    63901 cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta
    63961 tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa
    64021 aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat
    64081 ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt
    64141 gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt
    64201 gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt
    64261 tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt agtccagaag
    64321 ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac
    64381 gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa
    64441 ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg
    64501 ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta
    64561 atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca
    64621 catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga
    64681 catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc
    64741 cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt
    64801 gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt
    64861 caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat
    64921 tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata
    64981 tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact
    65041 gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag
    65101 agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag
    65161 attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa
    65221 aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag
    65281 ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa
    65341 aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg
    65401 gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt
    65461 tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg
    65521 agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa
    65581 tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt
    65641 aattgtcata agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt
    65701 ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga
    65761 aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa
    65821 tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg
    65881 aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg
    65941 agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt
    66001 taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat
    66061 tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag
    66121 attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat
    66181 tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata
    66241 ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa
    66301 cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt
    66361 tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca
    66421 atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt
    66481 ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag
    66541 ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt
    66601 ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc
    66661 tagctattga agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt
    66721 taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa
    66781 tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc
    66841 cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct
    66901 tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc
    66961 atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag
    67021 cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat
    67081 gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga
    67141 agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt
    67201 gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc
    67261 agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga
    67321 agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa
    67381 taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt
    67441 aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt
    67501 tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct
    67561 acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat
    67621 tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc
    67681 ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag
    67741 tgccaggtaa tccttttaag tcagtaatct caagaccagc agctttatgt tgctcgtgta
    67801 atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg
    67861 caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat
    67921 aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg
    67981 ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca
    68041 ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa
    68101 atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac
    68161 tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg
    68221 attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa
    68281 tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga
    68341 aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt
    68401 cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata
    68461 atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga
    68521 ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc
    68581 accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat
    68641 cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca
    68701 ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca
    68761 tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct
    68821 ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc
    68881 gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt
    68941 gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt
    69001 tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga
    69061 tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga
    69121 ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc
    69181 attacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct
    69241 gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa
    69301 aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg
    69361 atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct
    69421 cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt
    69481 tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat
    69541 tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct
    69601 tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga
    69661 ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga
    69721 tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca
    69781 gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat
    69841 tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt
    69901 ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc
    69961 aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc
    70021 agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg
    70081 cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact
    70141 tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg
    70201 gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat
    70261 gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca
    70321 tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca
    70381 ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt
    70441 tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca
    70501 aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc
    70561 agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa
    70621 aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta
    70681 tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt
    70741 tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta
    70801 tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc
    70861 ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg
    70921 ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata
    70981 cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc
    71041 aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc
    71101 aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccac gtttttcctg
    71161 attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc
    71221 gataccggca aattttttgc catggatgct taaatcataa gttccaggac aataagaatg
    71281 ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat
    71341 ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga
    71401 aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg
    71461 agcgtaacct gcatcttcca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc
    71521 cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg
    71581 ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg
    71641 ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt
    71701 catattttct acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct
    71761 gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga
    71821 agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt
    71881 acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg
    71941 gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat
    72001 gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga
    72061 gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca
    72121 ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac
    72181 cgtcccacag cagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc
    72241 ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt
    72301 tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt
    72361 agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc
    72421 ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat
    72481 cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat
    72541 attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct
    72601 atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca
    72661 cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat
    72721 tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt
    72781 gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc
    72841 ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta
    72901 agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct
    72961 gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag
    73021 aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag
    73081 taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt
    73141 gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa
    73201 aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca
    73261 cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata
    73321 aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc
    73381 ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact
    73441 tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc
    73501 atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg
    73561 aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa
    73621 cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt
    73681 gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca
    73741 attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt
    73801 ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta
    73861 atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta
    73921 cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa
    73981 aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt
    74041 catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa
    74101 gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta
    74161 gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat
    74221 gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt
    74281 ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc
    74341 gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg
    74401 acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat
    74461 caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa
    74521 tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat
    74581 tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata
    74641 agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta
    74701 ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag
    74761 taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat
    74821 caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag
    74881 cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag
    74941 atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac
    75001 cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag
    75061 aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg
    75121 gcaatcacac atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg
    75181 aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc
    75241 gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg
    75301 aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga
    75361 tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga
    75421 ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag
    75481 ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc
    75541 ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg
    75601 aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac
    75661 tgtccaccaa tt
//
LOCUS       DAYYNQ010000003        72221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000003 DAYYNQ010000000
VERSION     DAYYNQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.003"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHBJ1_000156"
     CDS             <1..1087
                     /locus_tag="ACHBJ1_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429133.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHBJ1_000157"
     CDS             complement(1305..2447)
                     /locus_tag="ACHBJ1_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429134.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHBJ1_000158"
     CDS             complement(2452..2637)
                     /locus_tag="ACHBJ1_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9429135.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHBJ1_000159"
     CDS             complement(2991..3236)
                     /locus_tag="ACHBJ1_000159"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429136.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHBJ1_000160"
     CDS             complement(3230..3631)
                     /locus_tag="ACHBJ1_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9429137.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHBJ1_000161"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="ACHBJ1_000161"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM9429138.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="ACHBJ1_000162"
     CDS             complement(5649..7415)
                     /locus_tag="ACHBJ1_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFM9429139.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="ACHBJ1_000163"
     CDS             complement(7428..8222)
                     /locus_tag="ACHBJ1_000163"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9429140.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="ACHBJ1_000164"
     CDS             complement(8232..9608)
                     /locus_tag="ACHBJ1_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429141.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="ACHBJ1_000165"
     CDS             complement(9728..10375)
                     /locus_tag="ACHBJ1_000165"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM9429142.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACHBJ1_000166"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="ACHBJ1_000166"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9429143.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_000167"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_000167"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9429144.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="ACHBJ1_000168"
     CDS             complement(13042..14673)
                     /locus_tag="ACHBJ1_000168"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9429145.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="ACHBJ1_000169"
     CDS             14977..15648
                     /locus_tag="ACHBJ1_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9429146.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15808..15999)
                     /locus_tag="ACHBJ1_000170"
                     /pseudo
     CDS             complement(15808..15999)
                     /locus_tag="ACHBJ1_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(16367..16708)
                     /locus_tag="ACHBJ1_000171"
     CDS             complement(16367..16708)
                     /locus_tag="ACHBJ1_000171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429147.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            complement(16805..17257)
                     /locus_tag="ACHBJ1_000172"
     CDS             complement(16805..17257)
                     /locus_tag="ACHBJ1_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429148.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="ACHBJ1_000173"
     CDS             complement(17264..17812)
                     /locus_tag="ACHBJ1_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM9429149.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="ACHBJ1_000174"
     CDS             complement(17857..18189)
                     /locus_tag="ACHBJ1_000174"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429150.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18460)
                     /locus_tag="ACHBJ1_000175"
     CDS             complement(18203..18460)
                     /locus_tag="ACHBJ1_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429151.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="ACHBJ1_000176"
     CDS             complement(18655..19197)
                     /locus_tag="ACHBJ1_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM9429152.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="ACHBJ1_000177"
     CDS             complement(19218..20309)
                     /locus_tag="ACHBJ1_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9429153.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="ACHBJ1_000178"
     CDS             complement(20317..22527)
                     /locus_tag="ACHBJ1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9429154.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="ACHBJ1_000179"
     CDS             complement(22543..25071)
                     /locus_tag="ACHBJ1_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9429155.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="ACHBJ1_000180"
     CDS             complement(25123..25533)
                     /locus_tag="ACHBJ1_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM9429156.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="ACHBJ1_000181"
     CDS             complement(25546..25764)
                     /locus_tag="ACHBJ1_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM9429157.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26585)
                     /locus_tag="ACHBJ1_000182"
     CDS             complement(25761..26585)
                     /locus_tag="ACHBJ1_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9429158.1"
                     /translation="MSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFD
                     NHEQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYD
                     IRSMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTL
                     YETVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYK
                     ETKKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26566..26835)
                     /locus_tag="ACHBJ1_000183"
     CDS             complement(26566..26835)
                     /locus_tag="ACHBJ1_000183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429159.1"
                     /translation="MNGLFFRKTSLSLSIVRTSKNILEGVAYEKTTTTAERKSLYRES
                     TEAKENPFKKSSEMDLVFGFISLFFRGLGIFSIHENEGSSVVFER"
     gene            complement(26832..27743)
                     /locus_tag="ACHBJ1_000184"
     CDS             complement(26832..27743)
                     /locus_tag="ACHBJ1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9429160.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="ACHBJ1_000185"
     CDS             complement(27762..28106)
                     /locus_tag="ACHBJ1_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429161.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28404)
                     /locus_tag="ACHBJ1_000186"
     CDS             complement(28126..28404)
                     /locus_tag="ACHBJ1_000186"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429162.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(28763..29263)
                     /locus_tag="ACHBJ1_000187"
     CDS             complement(28763..29263)
                     /locus_tag="ACHBJ1_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429163.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="ACHBJ1_000188"
     CDS             complement(29264..29809)
                     /locus_tag="ACHBJ1_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429164.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="ACHBJ1_000189"
     CDS             complement(29806..31062)
                     /locus_tag="ACHBJ1_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9429165.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31422..31733)
                     /locus_tag="ACHBJ1_000190"
     CDS             complement(31422..31733)
                     /locus_tag="ACHBJ1_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429166.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31756..33231)
                     /locus_tag="ACHBJ1_000191"
     CDS             complement(31756..33231)
                     /locus_tag="ACHBJ1_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM9429167.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33680..34132)
                     /locus_tag="ACHBJ1_000192"
     CDS             complement(33680..34132)
                     /locus_tag="ACHBJ1_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429168.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34134..34499)
                     /locus_tag="ACHBJ1_000193"
     CDS             complement(34134..34499)
                     /locus_tag="ACHBJ1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429169.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34598..38881)
                     /locus_tag="ACHBJ1_000194"
     CDS             complement(34598..38881)
                     /locus_tag="ACHBJ1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM9429170.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39028..39267)
                     /locus_tag="ACHBJ1_000195"
     CDS             complement(39028..39267)
                     /locus_tag="ACHBJ1_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429171.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39357..39599)
                     /locus_tag="ACHBJ1_000196"
                     /pseudo
     CDS             complement(39357..39599)
                     /locus_tag="ACHBJ1_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39712..39963)
                     /locus_tag="ACHBJ1_000197"
     CDS             complement(39712..39963)
                     /locus_tag="ACHBJ1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429172.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40959..41870)
                     /locus_tag="ACHBJ1_000198"
     CDS             complement(40959..41870)
                     /locus_tag="ACHBJ1_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429173.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="ACHBJ1_000199"
     CDS             complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="ACHBJ1_000199"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM9429174.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44048..45268
                     /locus_tag="ACHBJ1_000200"
     CDS             44048..45268
                     /locus_tag="ACHBJ1_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429175.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45288..45782
                     /locus_tag="ACHBJ1_000201"
     CDS             45288..45782
                     /locus_tag="ACHBJ1_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9429176.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45760..46446
                     /locus_tag="ACHBJ1_000202"
     CDS             45760..46446
                     /locus_tag="ACHBJ1_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429177.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="ACHBJ1_000203"
     CDS             complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="ACHBJ1_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM9429178.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49457..50503)
                     /locus_tag="ACHBJ1_000204"
     CDS             complement(49457..50503)
                     /locus_tag="ACHBJ1_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM9429179.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50514..50786)
                     /locus_tag="ACHBJ1_000205"
     CDS             complement(50514..50786)
                     /locus_tag="ACHBJ1_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM9429180.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50821..51729)
                     /locus_tag="ACHBJ1_000206"
     CDS             complement(50821..51729)
                     /locus_tag="ACHBJ1_000206"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9429181.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="ACHBJ1_000207"
     CDS             complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="ACHBJ1_000207"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM9429182.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53108..54229)
                     /locus_tag="ACHBJ1_000208"
     CDS             complement(53108..54229)
                     /locus_tag="ACHBJ1_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM9429183.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54226..54927)
                     /locus_tag="ACHBJ1_000209"
     CDS             complement(54226..54927)
                     /locus_tag="ACHBJ1_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM9429184.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(55071..55511)
                     /locus_tag="ACHBJ1_000210"
     CDS             complement(55071..55511)
                     /locus_tag="ACHBJ1_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM9429185.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="ACHBJ1_000211"
     CDS             complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="ACHBJ1_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM9429186.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(56566..57939)
                     /locus_tag="ACHBJ1_000212"
     CDS             complement(56566..57939)
                     /locus_tag="ACHBJ1_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9429187.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="ACHBJ1_000213"
     CDS             complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="ACHBJ1_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM9429188.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKTLRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(58765..59097)
                     /locus_tag="ACHBJ1_000214"
     CDS             complement(58765..59097)
                     /locus_tag="ACHBJ1_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9429189.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(59149..59778)
                     /gene="coaE"
                     /locus_tag="ACHBJ1_000215"
     CDS             complement(59149..59778)
                     /gene="coaE"
                     /locus_tag="ACHBJ1_000215"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM9429190.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(59742..60578)
                     /gene="mutM"
                     /locus_tag="ACHBJ1_000216"
     CDS             complement(59742..60578)
                     /gene="mutM"
                     /locus_tag="ACHBJ1_000216"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM9429191.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(60623..63268)
                     /gene="polA"
                     /locus_tag="ACHBJ1_000217"
     CDS             complement(60623..63268)
                     /gene="polA"
                     /locus_tag="ACHBJ1_000217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM9429192.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(63394..64077)
                     /locus_tag="ACHBJ1_000218"
     CDS             complement(63394..64077)
                     /locus_tag="ACHBJ1_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM9429193.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(64070..64555)
                     /locus_tag="ACHBJ1_000219"
     CDS             complement(64070..64555)
                     /locus_tag="ACHBJ1_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM9429194.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(64862..66196)
                     /gene="murC"
                     /locus_tag="ACHBJ1_000220"
     CDS             complement(64862..66196)
                     /gene="murC"
                     /locus_tag="ACHBJ1_000220"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM9429195.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(66327..67088)
                     /locus_tag="ACHBJ1_000221"
     CDS             complement(66327..67088)
                     /locus_tag="ACHBJ1_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9429196.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(67104..67586)
                     /locus_tag="ACHBJ1_000222"
     CDS             complement(67104..67586)
                     /locus_tag="ACHBJ1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM9429197.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(67606..69786)
                     /locus_tag="ACHBJ1_000223"
     CDS             complement(67606..69786)
                     /locus_tag="ACHBJ1_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM9429198.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(69930..70661)
                     /locus_tag="ACHBJ1_000224"
     CDS             complement(69930..70661)
                     /locus_tag="ACHBJ1_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9429199.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(70730..71671)
                     /locus_tag="ACHBJ1_000225"
     CDS             complement(70730..71671)
                     /locus_tag="ACHBJ1_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM9429200.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(71851..72219)
                     /locus_tag="ACHBJ1_000226"
     CDS             complement(71851..72219)
                     /locus_tag="ACHBJ1_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM9429201.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        21127 a        15163 c        11246 g        24685 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gacctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctgaaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt ttaacccttt agaaacaccg atatatcaac tagtccccct
    31141 cgttttttgg gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt
    31201 ttataaaact tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg
    31261 ctttacacaa agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta
    31321 gcgagtgcgc attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc
    31381 ggctaccaga ctacgtctag agccctcccg atagataata actacaaaat taaatgatct
    31441 aattttgtta tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa
    31501 tcctcttcta aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc
    31561 gttagcaata cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg
    31621 taatcagtta cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa
    31681 ggattaatca ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt
    31741 cacctcctta gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt
    31801 tgctaaatac atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt
    31861 ttgatcttga ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc
    31921 aaattctttg ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac
    31981 atcaaatcct ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt
    32041 tttaaaatct tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat
    32101 acggaatgtt aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat
    32161 tgctaaaata atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt
    32221 atcgactcta tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa
    32281 aaagataaaa tgcggtggta acccataata agcataattt ttccctggtt catatttagg
    32341 atctttcatg gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa
    32401 acaaataatc atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc
    32461 acctaatgct gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa
    32521 acaagcttga attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat
    32581 caacatattt ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc
    32641 attttctgct atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt
    32701 gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa
    32761 atgacccatt tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac
    32821 taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg
    32881 tttctcacta acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag
    32941 taactgacgc caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa
    33001 ccctaaaatt aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag
    33061 atacatttgc caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca
    33121 acctaacaga atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg
    33181 attgaaatag cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat
    33241 tcctcctttc tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac
    33301 aggaatatca catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg
    33361 gtgtgcacac atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg
    33421 tttccactgg tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat
    33481 aaccgcacaa atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc
    33541 aatcaattgt tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta
    33601 tctgaattca aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc
    33661 ctattctttc aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt
    33721 tttgatctaa acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta
    33781 ctttctctaa tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg
    33841 ctggaatata taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa
    33901 aaccagtcaa atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg
    33961 gatttccaaa aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt
    34021 cattatcaaa tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag
    34081 tttcatctaa tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa
    34141 acctccccaa tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga
    34201 cgctctgttt gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca
    34261 aaataagctt cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc
    34321 tcttcagctg tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt
    34381 aattgaacat atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa
    34441 cccaataaaa atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata
    34501 aagtttttcc tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa
    34561 caagcggtta aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt
    34621 tataaagtgt tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac
    34681 tacttgtttg ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt
    34741 gttctggttt cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt
    34801 tgtttgttag atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg
    34861 ttttcgcttg gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca
    34921 ctaagaattc aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat
    34981 gagtgccttc aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga
    35041 tttgaccaat cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg
    35101 ccaagatctc ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga
    35161 ttttgatggt ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc
    35221 taaacagatc ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa
    35281 agtctagatt gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt
    35341 cttttccatc tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg
    35401 tgtatgtttt tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg
    35461 ttaatggatt aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg
    35521 ggttagttac tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt
    35581 catcatttct aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat
    35641 caaaggtaaa tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg
    35701 tttctaccgt atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc
    35761 ctttgaccaa gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg
    35821 taaacaatcc tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa
    35881 cggttggatt attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa
    35941 cttcaatgcc atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac
    36001 tagcattaac cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca
    36061 aggtacttgt gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca
    36121 atactcctga aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt
    36181 taatgacgat cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt
    36241 gtgtttctag ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat
    36301 catctggcgc attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga
    36361 aactttctaa tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta
    36421 aaatttcccc gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca
    36481 aggtaaagtt cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata
    36541 attttacact tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat
    36601 cattggtaat cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga
    36661 tttcttccaa acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc
    36721 ctttttcatt ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga
    36781 cataacgatt ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt
    36841 ttcctgtttc ttgatccact ttcaccactt taagacgttg ttcaattact ttatcttcta
    36901 aaacataatg gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt
    36961 tatacccttc ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt
    37021 taccaaaagt agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg
    37081 tttttgacgt caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg
    37141 aatcgactaa atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat
    37201 cttttgcggt cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag
    37261 aaagcacata accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat
    37321 ctttaatttc tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt
    37381 tcccatctgc ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt
    37441 tcacaccagt ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta
    37501 aattaataac agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg
    37561 gacgacgata ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt
    37621 tatccggtgc ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga
    37681 caccttttgg tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt
    37741 tcaacgtttc gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa
    37801 agttattaat caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt
    37861 tgattgggat ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt
    37921 catcggattt gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc
    37981 gtacatttga atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg
    38041 cggctgagac accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca
    38101 tataatttaa agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc
    38161 aatcaatttt ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac
    38221 gattggttgg tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt
    38281 ttttcttttg attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt
    38341 gactagctgc ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt
    38401 cagcagtagc cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt
    38461 tctcagaaga ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag
    38521 tggacgtttc tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac
    38581 caggactatt ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat
    38641 tgaccgttgg cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc
    38701 cggcttctgc tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg
    38761 taggttgtat agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga
    38821 attgagaaaa agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca
    38881 caagaattcc tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag
    38941 tgataacgac gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc
    39001 atcaatgatt ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa
    39061 atcaactgtc catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa
    39121 tctcatgcca taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc
    39181 ttcatctaat gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag
    39241 atcttctgga agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc
    39301 attactagct actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat
    39361 tcttctttta cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta
    39421 aaaccatcta ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa
    39481 taggtaggtt tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca
    39541 aaataactat ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt
    39601 attgatactt gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat
    39661 aaatattccc gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc
    39721 cccattcgta taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc
    39781 gtcccaacca atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa
    39841 tgccataagt tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac
    39901 acgtgtgcct tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg
    39961 cattttttgc tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct
    40021 tctttataaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40081 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40141 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40201 tttactagaa cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa
    40261 aaacgataga tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc
    40321 attgcttatc aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga
    40381 ccgtacgaat ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa
    40441 taaccctatc ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact
    40501 atgaccatta ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga
    40561 tctccgaatc tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat
    40621 gtggctctgt acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc
    40681 aagcgaactt tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca
    40741 ttttatctag ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc
    40801 cattgagctt gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa
    40861 ccctttttgt ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa
    40921 aacgtcgata acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat
    40981 tctaaagaaa gatttcctat ataagcatat tggataattc gattagtatt gggtatatct
    41041 ctaccattct catacctatc tatggaccat tttgaaattg aaggagaaaa aagctttcca
    41101 aactcctccc tttctagata ataatctttc ctaattttcc tcatccttaa tcccatacta
    41161 aaagaattta tctttttaaa atcgctaact ctttcatctt ttggaagtat attcttataa
    41221 tcttctccgt acataaggta aggaacagtg accttaccaa gataggcaat attcattagt
    41281 ttttcaatat ctggaatatt aatacctttt tcccatctac ttataacatt tttgcctgta
    41341 tagatggtaa agtttttacc aaatttttca agcgtctctt cccggttctt tcggacttct
    41401 cgtattcttt ttcccataac aattttatcg tcttttgata aactaaatat ctcactatca
    41461 cgattaatct ccttcaaaat ttctttttca ttacttaata gaaaaatatc gatttttttt
    41521 tgcatttctt ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta
    41581 attcctatat tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt
    41641 agtttaccga tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc
    41701 agaatcttgc ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat
    41761 ttaactaaag tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt
    41821 ttctcttttc ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca
    41881 cctcctatga gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc
    41941 aattttgtta attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca
    42001 gataatcctc agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt
    42061 ataagttatt tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc
    42121 ggaacttcga cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag
    42181 agtttacgtt tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag
    42241 gctttgatgt ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca
    42301 aattgttggc gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag
    42361 gcaaagtctc ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg
    42421 tccatcttca ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag
    42481 cctttcgtgc ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata
    42541 ttatagacga catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt
    42601 tttcgttgag aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct
    42661 ttgacgaatg gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta
    42721 tctactgtag cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc
    42781 gtgatcaatt ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga
    42841 agtccgagga atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat
    42901 tgtaaagcag catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga
    42961 gaagtatcaa ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt
    43021 aaagcttcag cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg
    43081 acagttttga tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca
    43141 actgcttttg gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc
    43201 agtttgattt tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca
    43261 ccgcggtaac tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct
    43321 ccacttggtg caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct
    43381 gatttggcac tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct
    43441 tgacggacac gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc
    43501 tccaaatcat tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt
    43561 gctgcatcga ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag
    43621 gtgaagtcga cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc
    43681 gctgtgtaat tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa
    43741 tccatcgagt ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct
    43801 aaaatacgat cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta
    43861 cgaatctgct cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata
    43921 taatcagcac tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc
    43981 agctatagaa gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga
    44041 tgaaaaaatg gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga
    44101 acatatcgat caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat
    44161 cgaggaaagc gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga
    44221 agtatcgaaa gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta
    44281 tccttgtgaa gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg
    44341 aacttatcca gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga
    44401 taatatgcgc aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta
    44461 tcggcgtaaa ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca
    44521 gctacctaaa caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga
    44581 cgtattttcc gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga
    44641 ttttcattgg gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta
    44701 ctacgatagc aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt
    44761 tcacgtaaaa gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat
    44821 ttccgcacat ttttccatga tctactgggt acatggagat atttataaaa atgagccttt
    44881 agcatttttg attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg
    44941 gatcgttgat gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt
    45001 tcatttcatc attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg
    45061 ggatgaacag ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga
    45121 tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat
    45181 cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat
    45241 ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt
    45301 ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa
    45361 tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg
    45421 acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg
    45481 ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg
    45541 aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac
    45601 agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat
    45661 ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt
    45721 cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt
    45781 aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat
    45841 aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg
    45901 gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa
    45961 cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt
    46021 ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg
    46081 gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat
    46141 gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga
    46201 ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat
    46261 cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat
    46321 acgatgggcg acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg
    46381 tctcaaagta aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat
    46441 acctaataaa agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc
    46501 gaagaaacta cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt
    46561 cagacccctt ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc
    46621 tgttttgaaa tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac
    46681 atgtcctgca acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt
    46741 caatggtctt gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc
    46801 atcactgatt gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat
    46861 tttgtcaacg attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc
    46921 gattcggttc aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc
    46981 cactgcttct ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat
    47041 attgctggtc atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt
    47101 caagcgtccg tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc
    47161 gatttcatcc aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc
    47221 accttcttcg taacctacat agcctggagg agctccgact agacgagaca cagcatgttt
    47281 ttccatatat tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc
    47341 agctaacgct ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga
    47401 acctagagga cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac
    47461 cgcatcaact gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat
    47521 tagtttttct cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac
    47581 gacttgagca atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt
    47641 tgctttagct tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa
    47701 tacagcagcg cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt
    47761 atcgatttca gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg
    47821 catcttcatc gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa
    47881 gcgtttttta cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg
    47941 catcaaacgt ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat
    48001 cgttgcactg gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat
    48061 ataacggtca gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg
    48121 gtggatctca aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg
    48181 ttctttgact aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg
    48241 acggtactca tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg
    48301 tttcaacaga tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg
    48361 gatttcatcg atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc
    48421 ttttagtctt tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc
    48481 taatgaaaaa atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg
    48541 agcaagccct tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt
    48601 attttttgtt tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat
    48661 gattggatcc atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa
    48721 tgctttgtat tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc
    48781 gatgttttta cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg
    48841 ataatttttt aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc
    48901 gaagctttca cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt
    48961 ttgtccatat tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat
    49021 ttcatgttca aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg
    49081 attagattgt aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt
    49141 gaccgcaatc tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat
    49201 attcatacag gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca
    49261 aaaactatta tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt
    49321 ttttctcgac ttatataaat cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga
    49381 gccaaacaaa cgtaaaatta tcttatccat aacgtttttc tttgatttga taaaaagggt
    49441 agatcttata aaagacctac ccttgatttt ctttattctc tttttttact tctattattt
    49501 cttctttgat cactcttcgc cgttcagcaa tcacatacac aaatccatag agaaatggca
    49561 agataaagga actgaaacga tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa
    49621 ctggtctgaa taataacaaa tagacaaatt caaaggaacc aataccagca ggcgtaggaa
    49681 taacccctga aagcactact gcaaagctga taaacgaaat ggtcagtaga aaatctattt
    49741 taggatggtt ttcaatcaga atcaaataag gaagtacata ccaaaataca agttttaaca
    49801 tattccatag ataaatccgc aatagcgcag tcctgtcttg gataatggtt tggaccgttt
    49861 cccttaaaga gtagatctgc tgattgcatg tatccaccca tttccgcaaa cgctcacttt
    49921 taaataactt attcgataag ctgacaaaaa atatttgaac attcaagctg acggacatcg
    49981 ccaataaaaa ggcaacaatc acgatcgtca aaaggatacc agcgatgata aaccagatca
    50041 ttgatggacc attagaataa aaaagagaga attggatgac caagccagct aatgcataag
    50101 tgatcactgc cagtttgtac ataatcatat ggagtgctgt cacccctgct ccctgggata
    50161 gcttcattcc ttttttgtta taaaaataaa tctccgagat cagcgttcct gtaccaaacg
    50221 aaacgattcg gtaaaacgca atatagctag aggtcaagaa cccatctttc gttgtaaaat
    50281 tcggcgcgaa aggttcagca atttctttga ttgagcgccc ttcagcgatt tggtaaactg
    50341 tccctaatat aacagccgct gccaaaacaa caacactggt agacagcaat tcggcaaaga
    50401 tatctgcaaa tgattgattg attaaataat aaataatggc aaaaatcaca atcagcaaaa
    50461 gcagattcag aatgatttta gttttcgttc ctttagacga catctcctgc tccttaatgg
    50521 aaaaaataaa tgagaatcaa atcaacaagt acacctgcag cgaatcctat agtaaacttc
    50581 tttttccgag ttttatgatg gaaaactttt cgagagatca atcctcccaa accaccgcct
    50641 aacaggctga caaatatcaa attattctca ggaacacgcc agccgctagt tctagcctga
    50701 tgtttatcat accccatcag acaaaataag taaaaattca caatgaaaag gtaaaaccac
    50761 aaaggattgt gtaacaatac atccattact cttcttccaa ctttctgtta actactattt
    50821 ctaagctttt tcttctttct ttggtgaata aactgttaat ccttttgata caacagataa
    50881 ttcaactgga agatcatctg tcgtgtctcc atccgttcga gtgcccacag atttttcctg
    50941 cgcttggatt ttcatgcgtt tggctgtgaa ataagtaatg ccggggatcg tatagaattt
    51001 gcctctcatg attctaggca aataaaaaat aaatcggaaa atatctaagg aaggtacgat
    51061 cgttacatga aattttccat catctagggt cgctgagtcg tcaaaatttg taaaaccgcc
    51121 cacggagtta gacatcgtga ctgtcaacag ttgggatttt cctttccaat gctcctcgtc
    51181 tgtttctaaa tccaaatgat attttttctt cttcagcaat aacttaaaaa acttcttgat
    51241 aaagatccac gagccgtaac gctgtttttc ttcttgactg atccaaactg ctgtatctgc
    51301 taagattccg attgtcagtg tagagataat gacttcatca ttgacttgac catagtctat
    51361 tttccgtaca ttgccttcca ggatcacgtc agcagcttct tcttttttga ggggaatctc
    51421 aagaacacgt gcaaggttgt tgacagttcc acctggaagt aatccgacat ggtattgaga
    51481 taatgttttt tggaacgctt ggacgatatg atgaatcgtg ccgtctccac ctataattac
    51541 taaagtatca gcttctatgg cttctgcttt cttgactaat tccgcatcat caatcgatgg
    51601 gccagtttca gctagttcta catataattc tggctgcttt tcttttacat aatcttgaaa
    51661 ccagcgtgct aatttctcac cctcatcttt ccctgaactt ttgttataaa aaactagcag
    51721 cttttccatt ctttacctcc tatttactct tcaaaaaaga gggcgggaca accaattcca
    51781 ttgtcctgcc ctttagcctt agacagatga cattccatca ttgggacgaa cgccttgtca
    51841 taagctgttc tttttcgatt ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg
    51901 tctgttgctt tttccattac ttgtaaagaa acaaattcga atcgtccgtg catattttct
    51961 cctccggcaa atagatttgg cgtcggaata cccatgtaag agatttttga accatctgtt
    52021 cctccacgaa ctggttcgat cactggagag ataccaagct cgatcattgc ttgtttcgct
    52081 aattccacga tgctcatatc tttttcaatg atttctttca tgttgtaata ttgatcatac
    52141 atatctactt tgattcgttc ttgatcaaat cgctgattca actcttcttg gatatgtgtg
    52201 atttgtgctt tgcgtgcttc gaatttttca cgatcatgat cgcgaatgat ataactcata
    52261 gaagcttctt caggattccc atccaatgcc atcaaatgga agaaaccttc gtatccttcc
    52321 gttttttctg gaacttcatc ggctggaagc tgattatgaa aatcgattgc taactgtaga
    52381 gcattgatca ttgtatcttt tgccgtccct ggatgtacat ttttcccttg gatcgtaata
    52441 tttgcttgag cagcactaaa cgtttcatac tgcaactcac ctaaaggacc accatcgact
    52501 gtataagcaa aatctacatc gaattgctca acatcgaatt tatccgctcc gacaccgatt
    52561 tcttcatcag gaccaaaagc cactttgatg gtaccgtgag ggatagaagg attattgatc
    52621 aagatctcca ttgcagtcat gatttccgca atccctgatt tgtcatctgc tcccagtaat
    52681 gtactgccat ctgttgtgat caatgtgtgg ccttcataat ttttcaaatt tggaaaatct
    52741 tttgtattca atgtatagcg ttcttccgca tctagtacga tcgtagactc gccatcatag
    52801 ttttcaatga tttgtggatt gactcctact gcattaaaat ctgctgtatc catgtgggaa
    52861 ataaatccaa tggaacgcac ttcatgatca acattgcttg gtaatgttcc cataacgaat
    52921 ccgttatctt cattatagaa aacatcagct aagcctaact cttctaattc ttttttcaat
    52981 tcttgcgcaa aagcaacttg tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag
    53041 cgtgtttctg ttttgacata tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc
    53101 tctcctttta attaaattga aatgggtttg tatttgtttc agaaacgaaa aagttaatat
    53161 cccaattttc ttcttgtttc caagattcaa atttctcgat aaatcgttgc ttgcataacg
    53221 attcaatata atgaccggga tcaattgcaa tcaacccagc gctttgcata tcatgagccg
    53281 tatgataata gatatctccc gtgatataga catctgctct ttgggcgatt gcttgcggat
    53341 aaaatttttc accgcttcca ccgcaaatcg caatgcgctt cacagaggat tttgcatttt
    53401 ttggctggac tattctcagt ccatctagtt ggaacgcttc ttttacttgt tctacaaatg
    53461 cctctataga aatttcttgc ggcaattcac ctactcgtcc taacccaaac atttcgactg
    53521 gttcatctat agcgaaaaga tcataagcag gttcctcata aggatgagct gaccgcatgg
    53581 cttgaatcac ttgtttttcg atagtttccg gcaaaataac ctcaactttt gcctcttgaa
    53641 cttgttctgt cttccctact ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc
    53701 gaccgtgtcc aatcgaggta aaacttgtac cggtataatc accttgtgta ccagcaccag
    53761 ctgcagctaa tacttcacgc attttctgtg catgatccac aggtacataa acggccagtt
    53821 ttttataatg aatctcatga gtctttacta aataactctc cacttcaata ccgagaagtt
    53881 cacaaaacca gtcattcagt ccatcccaaa taatatccat gttcgtgtgt gctgcataga
    53941 cggcaatatc atgtttcaac aaatctgcat acattttttc ttgtggctga tcagtaacca
    54001 gccgttttac aggacgaaag attggaggat gtttagcaat cagcaaatcg atttttttct
    54061 tgattgcttc ttcgaccact tctggacgaa cgtctaatgt catcattact cgttggattg
    54121 gcttatccaa tgttccgata tgcagtccga ctgggtctcc ttcttcagct agccactgag
    54181 gacaatagct ttcaaacttt tggatgaaag tgcgtccgct gattgtcatg gctttaaaac
    54241 ctcctcaatc atttggattt cctgctcgat ttgtttttga cgtgcctctt gattagctgc
    54301 ttgtttgagc tgttcacgga tagcttttct ttgttgaagc tctcttgtcc attttgattt
    54361 gaagatctca cttttctctt caagcagaaa tggcccgaac atcagctcca tttcattgta
    54421 agtaactggt ttttgttctt tttcggctac aatgatttca tatatttttt tattttcttc
    54481 aagtattttt tctgattgga ttttatactg attttcttgc agccaagatc gtaatgtacg
    54541 ctctccgatg tttggctgaa ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt
    54601 gccagcttct aggatatctc ggatcagtga acctcccata cccgcaatcg tgacagctgt
    54661 aataccatct tgtggctgga tagcatctag accatttgca aggcgtacgg tgatacggtc
    54721 ttctaatcca tcttttttga cttgcttacg tgctgattca taaggacctt gaacgacttc
    54781 accagcaact gcatattcga tttttcctct taatatcaat gcaacgggta agtaagcgtg
    54841 atctgatcca atgtctgcta aacgagcgga ttcagggaca aagtcaccca ctgcggctaa
    54901 gcgctgagat aaatctaaat ggttcatagc ttcactcctt ctagataatt atagcatttt
    54961 ccaaataaaa taaggagact aggaataaaa aaattcccat atctccttaa aaaatatacc
    55021 ttttcgttct ataaaacaac attttcttat ttttgaaaaa atgttattgg ttatttgttc
    55081 aatgaaacat caataatcga atctccagga acttgcggtg ggatagacat cgtcatgatc
    55141 ggcatctcaa ctaatgtaaa ttggactttg tcaccgattt tcaattcatt tatgtccacg
    55201 cttatttttt cagcagtagc atttaagatc actccgtcat tgctgaatga cgttcccata
    55261 ttcgctggat cttctttggc catgacatcg acgagtttca actgaagcgt ttcttcttct
    55321 gcttctactt gagtgatact ttcaatcgtt ccgacaaata tgctttgtct ctccgttttc
    55381 gtactgcttg tttttactgt ttgacccgca tcaatcgaat caacagaacc tgtgttgcta
    55441 tctgcgctac cgcagcctgc taaaagaaaa aatagaccaa ctacagatat actagaaaaa
    55501 atagccctca taaaaaatcc tccgtttaag tttctgcatt acttgttgcg gtgattgtct
    55561 ccggtcattt ctgtccatat aatgattcgc tgacctgctt ttactactag gtcaaatgtc
    55621 agttggactt gtcaactgtt caaccattgc gtcggttgcg accagtcatt tcgatttctt
    55681 ttgtcaaata acgtatgcgt tccattatcc gtttcgcttt tagcggctct tcatctccac
    55741 gttgagtgac ggacaaatgc tgttccaatt catccattgt aaaattcgat gagaagaagg
    55801 ttggtaactg ttcctgcatc cgatattgaa ggataacacc gaatacttcg tctcgtatcc
    55861 aactactcat cgcatctgca ccaatgtcat cgatcatcaa aataggtgaa cgtttcactg
    55921 catctaactt ttctgccact tgatcttttc cgattgcctg tttcatctca acggcaaatg
    55981 tcggaaaatg gactagcgtc gtggtgaatc cagcaatcgc taattctctc gctgttgctc
    56041 ctaataaata tgtttttcca ataccaaaac ttcctactag atacagtcct ttatgatacc
    56101 cttttggatc agcagtatat ttctctacaa aatctagggc ttctaagctc gcatatcctc
    56161 gtccatctgt acgttcaaag ttttcaaaag aagccgattg aatatctttt ggcatatcca
    56221 ttgcttttac tcggcttctg atttcttctt gtctttgccg tgctaacaat gcttccgttg
    56281 gtacataagt gacatccaca taatgaaaat tcaacgtcag cttcggctca tagccgggag
    56341 cgatcatcgt tggatcgttt ttttgatatt tacgcttttc ttgcacgaat tcatacaatt
    56401 tagcgtaact tttttcaata tccgcttgtg tcaattcttc ttggtgtgcg cttagaaaag
    56461 cttgaacatc cgcatcattt aagacttcag cgatcatttc agcatacttt tcttgataat
    56521 tgcgtttgct gatgatcctc ttcaattctt tgcctacgtc ttccactaga tatcaccctc
    56581 cttttttgcc aggtattctt taaaacggcg gtcaatctct gcttttttct cgctgctgat
    56641 ctgtttttca tcttttggct ggttgaccca atcaggaagc ttttcccgtc gaacatttcg
    56701 cttgccagtt tgataatttg tttgcttctt ttgttttgat tgttttgctt gattacttat
    56761 ttgccgaatg tgctggatcg cttcttctgc tgtggcgatt ttcttttgtg cccattcatt
    56821 tgccactgta tttacataac tggcatttaa gctagcatta ttcttgatca ctagtacata
    56881 attcagcaaa acattgatca cgctactgga aagacctgat tgagaaacaa gatcttgcag
    56941 taaccagttt tcttgtttag aaacatatcc gcctttttct gacttgattg cttccagata
    57001 tttcatcggt ggaatgctct gtgcttcttt tatcagctga agcgctgcgt ttgtcaaatg
    57061 ttcgtttgac gagggttcat tactgttttc agaaaccgca actgtttctt ttgtttgctt
    57121 caccggacgt ctctgcattg ccaaaatggc tttttgcagt tctttttcag atactttttg
    57181 ttcactaaag tcataagatt gactgatcag ttcgccaagt gtcaattcat cgaaaccgta
    57241 aagctgatga tacatgtcga cttgtttgat gattggatca tcaagacttg caatatgttt
    57301 ttttgccagc caatcattca aaaactgcca atcgaatgtc gaatctgctt caagtaatgc
    57361 tactgattca gattcttcaa aagatttatt cagcgattcg atgttttctt gattagcagc
    57421 gtacactgct tcattgaagc gataaacatc taaaaatttt tttgtgattt tttgatatcc
    57481 ctctgctgta tgccttttag gttcaaagaa ttgggtaagc ttctggaatc gtcgttcacc
    57541 aactttttcc agaagaagaa aagccagaac agagtctttg aaaaatgctt taggaatgag
    57601 tggttctaac agctcataaa taaaacgagg tcccagatca ctatcttctt taaaataaac
    57661 atccagtaag ccgattcctt ctagcttttt tcttgcatca taaaattgcg ggataccgca
    57721 gcctagacta ctaagcagat ctgcatgcat cccttcagga cctttgccag attcttgaga
    57781 gatttctgat aacagggtga aatagatact caaggcttct ccgccgatta gtggctgata
    57841 aagatacagc aaggcgttct tgctgtcttc tctcagcgga gccgcttttc ttccttggta
    57901 aatatttttt ggttgaagct ctttccaagc attttccaag gaaatgcctc ctttttgttt
    57961 cttaattgtt ttctttatta ttctctttat tattttcttt tgctttatcc acgatatctt
    58021 gcaactcttt taagaaaaca gacatatctt tgaactggcg ataaacactt gcaaaacgaa
    58081 tataagcgat ttcgtctaaa tcaactaaat cttccatcac atattcacca attagattcg
    58141 tcgatacttc gttttctccg agactgcgta cgcggttttc gacatggttg acgatttgtt
    58201 ccatttgttc catagctaca ggtctttttt ctgctgaacg gatgagtcct cttaaagttt
    58261 tatcgcgatt gaactcttcg cgttccccgt ttttcttgat tactaataac ggagcagctt
    58321 caattcgttc aaatgtagtg aatcggaacg aacaattctc acattcgcga cggcgacgga
    58381 tcgcacgtcc atcatctgct tgacggctgt cgattactcg tgaattatta tgttgacatc
    58441 tgggacatct cattttgtct cacctcttgc atcgcatttt atataaagga tgtgtctttc
    58501 tttacaacta gttgtattat aacacgaaac aaatcacaaa cgcgactgtc ctacccaaaa
    58561 aacacggata taagaacaat tttttctact ttcatgcgta attgtgtccc ctgtccttac
    58621 taaatttcaa ttttccaata ctgatcacct tgattactga gaaataactt acacaaaagc
    58681 tgatttagtc atctagcaca aaaaacttga agcagatgaa caccttctca tctgcttcaa
    58741 gtcacatctc gattttttac tattttataa cttctcttcg attcctgtat agtgtttcat
    58801 tcgctgttcc tcgatttttt gctcatcggg aatgtggtca ctttctggga gcggtgtttt
    58861 actacgatca ggtgtcggat tttttcgttc ttgagtcaaa tcttgccgct tcgcttcttc
    58921 ttctgcctgc tggtcttcta tttcatcgat tcttgcttgg tactgctttt ctttttcttc
    58981 tgctgtttct gattgcgtac gaccttcatc gattagattt tctttattga gcatctcgcc
    59041 gattttttct tttgcgttac ctacgacttt atccttttga ttttttaatg atcccatcgc
    59101 tatccctcac tttctatata ctcaggatag caaaattgac acagatactc aaagaaagcg
    59161 attagcaaaa agccaatttt cgatttgtcc cacaagtatt tcttttgttc cattgttgtc
    59221 gaacaaaata tccgcacgct gctttttttc ttcgattgga agttgactac taattctctg
    59281 ctgcgcttgt tctagcgtca gttgattacg tttcatcaaa cgctgaagct gagtttctgg
    59341 cgtagtataa acgacagcta ctgcatccat atagtggtca taatgacctt cgtagagcaa
    59401 tgggatatct acaattacta aaggatgatt ggaacttaga gaggcgattt gatcttttat
    59461 cgcttttcgg ataaacggat ctaatagctg atcaagtttt tgccgttttt cctttgatgg
    59521 aaaaacaatc atcccaagtt tccctcgatc tagttgccca tcaggctgca atacctcttg
    59581 gccaaacact gatacaattt tcctcagagc aagcgttcca ggctcaacag tttctctggc
    59641 aatcacatcg ccatcgatca aaggaaatcc atgagactga aagactttgg ctgccgtact
    59701 tttaccggta gctattccac ccgtcaaacc taaaacaaat cctactttgc gatttttagc
    59761 ctttgacaat tcggacaata gtgcgtcccc ctttgtgcca cttttgtttt cacgatcggc
    59821 gttccacaac gtatacatgg tttgccagtt tgctgataga catgcagtgc tacttgaaat
    59881 ccgcctgatt ctcctaatgc attcaaataa ctgcgaatcg tggtacctcc ggcttctatt
    59941 gcacgatcca aaacatcaat gatcgataga cgtaattgtt ctacctcttt ggggcgcagt
    60001 gtgttcgccg gttgttctgg atggatcttc gcttcccata atgcttcatc tacataaata
    60061 ttacctagac cagtcactag acgttggtct agcagcaatg gcttgattgc tttgtgggac
    60121 tttttcagtc catttgcgaa atctgctaat aagaagctat ctggcaaagg ctcaggtccc
    60181 aatttcatga ttcctcggta agttgcactt gctccttttt cgacgattgt catacgacca
    60241 aattttcgta catcgttgta gcgaagttgg cttccatcct caaaaaagaa aagtacatgc
    60301 gtatgtttat cgattggtgt attttctttc gtaaattgat attttccttc cattcgcaag
    60361 tgtgagatca gttcacaatg atcgagatga aaaattaaaa acttccctct acgtccaatg
    60421 gattggatgg tttcgccaac aagagaagcc tcaaagatgg gtgtctctgg ttgctcgatg
    60481 atcttgggcc acaagacctg aactttttga atcgtttttc ctactactaa ccgttccaat
    60541 cctttacgaa cggtttctac ttctggtaat tctggcattt tttcttttca ccttcattat
    60601 attgctctgt cttaaacaat gtttattttg cttcgtacca cgtttttccc caactgctgt
    60661 cagtgatcag tggtacatga agagaaacag cttgttccat aacttccttc accaatttat
    60721 ctaatgattc aagttctttt tccggtacct cgaagacaag ttcatcatga acttgtaaca
    60781 gcatcgtagc ttgaagacct gtttctttta atcgtttatc caattcgatc atcgcgattt
    60841 tcaaaatatc agcagcactt ccttggattg gtgtattgat agctgttcgt tccgcaaaag
    60901 aacggatatt gtaatttctt gaattgatgt ctggcagata tcgacggcgg tgatacagcg
    60961 tttcgacgta gccttggtct tttgcttcgc gtacgattct ttccatatat tctttgacac
    61021 ctgggtattt ttcaaagtac gtatcgatgt attgctgtgc tgcttttcgg ctgattccta
    61081 gattttgtga aagtccgtaa tcagagatgc catacacgat accaaaattc actgcttttg
    61141 cctgcctacg catatttggt gtgacatcct ctgccttttc gattccaaat acacgcatcg
    61201 ctgtgctggc atggatatct tgaccttcta ggaaggcagc tttcaaatgt tcatcatttg
    61261 agatatgcgc aagtacgcgt aactcgattt gtgaatagtc agaagaataa atcagccagt
    61321 ctttttctct aggaacaaat gcctgacgta tctttctccc ttcatctaat cgaatcggga
    61381 tgttctgcaa attaggatca acagagctta atcgtcctgt ttgggtcaat gtctggacat
    61441 aacgggtatg cacttttcca tctgattgga tcactttcag cagtccctcg acataggtag
    61501 attgaatttt tgcaatttga cgataggtga ggatatcctc tacgattggt gcttgttcac
    61561 gaagctgttc cagtacatct acggctgtgg aatatccagt ttttgttttt ttgataacgg
    61621 gtaatcccat tttttcaaat aagatcactc ctaattgctt tggtgaattc aaattgaatt
    61681 cttcgccggc ttcttcgtag attttttgtt cgatctctct taagcgagca gagaactcgc
    61741 ccttcatttc ttgtaagcga gtcgcatcta ctttgatccc gcttatttcc atctctgcca
    61801 aaatgataga caatggcagt tccattttcc ggaaaagatc ttcttggttt ttttctgcca
    61861 gttcttggct caattttggt gtcaacgcat tgattgcagc tacttttcgc gctaaatgag
    61921 caaaaaagag ttcctcttct tctggtaaac cttttttcgc ccctttgccg tatacagctt
    61981 catctgattg gatatcgttg tatccgtaat gagcagctat tccttcgata tctgtgcttt
    62041 tttcattcgt atcgagaagg tatgcaccta gcaagacatc aaaatcgatt cctttcgttt
    62101 ttccagtata gcggtttaaa gcaacataag ttcgtttcgc atcatatact tttttcagcc
    62161 gagtcggatc agtgatccaa gaatggaacg ctcggttttc gaatacagat agatcgttcg
    62221 tgacataaat tttcttttct gtcccccacg ctactccgac gatttcctct gtatgataat
    62281 tgtcgcccat catttccacg tacaacgcca tatccgtagt aaacatttcc tctttgaatt
    62341 catgaacgac ttcaaacaaa atatcatcca tttttacttc ttcttctacg atattcaatt
    62401 tagaaaggaa ctgattgaaa tccatttctt tataaaacgg aacgagttta tcgagatttt
    62461 tcccttcata cttcaatgag tcgatagaga catccacagg cgctgaagta ttgatggtcg
    62521 ccaatttttt cgataagaaa gcctgctctt tgtcatttat cagattttct ttcattttgc
    62581 tttgcttcat ttcatcgata tgttcataaa ttccttcgac tgagccatat tgtttcaata
    62641 gcttgatagc tgttttttca ccgattttag tcacaccggg gatattgtca gaggcatctc
    62701 cggctagtcc cttcatgtca ataatctgtt ttggcgtcaa cccatcatat ttttccgcta
    62761 catgttctgg tgtatagctt tcgatatcgc tgacaccttt tacagtgata tccactttta
    62821 cttctttact agctaattga gtcaaatccc gatcacctga aagcacgacg acatcaaatt
    62881 gatctttgtc tactttttct gccaaagtac cgataatatc atctgcttca taattgggca
    62941 attcgtaata ctgtacccct agtcctgtca acaaatcacg tatatatggc atttgttctt
    63001 tgaattcgcc aggtgttttg gatcttccac ctttgtactc cgcatacatt tctgtccgaa
    63061 aagtcgtttt tcctgcatcg aacgcaacca gaacatgagt cggttgctct ttttgcataa
    63121 cattttcaaa catcgtattg aatgcataga ttgcatttgt atgaagaccg tttctatttt
    63181 tgaaacgttc aagagaattg tgaagcgcaa agaatgcgcg aaaagccaca ctgttcccat
    63241 caacgagtaa taatttattt tttgtcatct tcttaactcc ttactgtaaa gttcttattc
    63301 attctaacaa agaatcctgc ggaatgcgaa aagaaaaagc gggaccatac caaataattc
    63361 ggtatagccc caactgatga atttttttct tttttagttg ttccgtccaa agattctcag
    63421 aaacgccaag aacaagttga taaagtccaa atacagcttc aatgccatga atacagcaat
    63481 tccactatgt gctgttccgt ttgtctggac atagatatta cggatgcttt ggttatcata
    63541 agctgtaatc cctgcaaaga tgacgactaa cagtagggag atgaacagct caactacgct
    63601 acttctcaaa atgaagatgt tcaagactag tgcaatgatc actccgatta aagctgcctg
    63661 tcctgctctt ccaaccccgc taagatctct ttttgttgtt actccgacaa cagacatccc
    63721 catgaacatc aaagtagctg tcaagaaagc ttgcgttact gttccaatat cgtagtatgc
    63781 aagagtaacc gctaatgtta tgccattcaa aagagagtag gcaataaatc caccgattgc
    63841 taagctcgga ttcttttgtg cttttacgcc tagaaccatt actaaaatca gttcaacgat
    63901 ccacagaccc acaaagccta gtggaaaatt attgataaaa gttgctaatt gcaaaggaaa
    63961 tacccaaaga gccaagtaag aagtgactgc actaagggca agtcccatac ctaaaaatcc
    64021 ataaactttt gcataaaaac gattgatacc tgacattaat gtacttggat tattcattca
    64081 attcaccttc actttcgatt gctatttcca attcaggaac ttgtggcggt tggaccataa
    64141 cgacagcatc caacacacga tcttcgttcg tattctccgc ttctacagaa atcgtcacca
    64201 catccttcat cttatctact tttatgacct caaactgaaa agtcagtgtt tcataatgga
    64261 aaaccggttc aataaaattg acagaaaaat tgacaacatg tgatcctggt ccaggaagat
    64321 gctttgaaat tgcacttgtg atgatcccca tcagcatcac tgatggaacg attggctgtt
    64381 gatattctgt tttttgtgca aagtcatgtt ggatatacaa aggatttgca tcattcgtca
    64441 atcccaaata cagcaaaagc tggttatctt caatagattc tgttaaggat aacgagtcgc
    64501 cttcctcgat ttcatttatt gtttttccta gttttctcgg cttacctatg ttcaagatag
    64561 aaaccctcca gtttattttt aatcatacat tattgtacca aaagtagcgt aaaaggcaag
    64621 ttctgctccc ttgatttaac agattcctat agtttacaga ggtcgtgaaa aagctgtcct
    64681 gcatcaaata ataagaacag ccaagaaaaa acagcttctc atgtttttga tttgattggg
    64741 cttattaccg cagatgcaag cttttgaaca ccgtttatca taagttgcaa aaaagggggc
    64801 ttggcaagat ttttgccact ccccctcttt ctatttttat aaggacactg cagtaattgt
    64861 attacagtac atttttcgtc gtgctgctta aaagtttttc atacgcttgt tcaaattttt
    64921 gaacatctcc agcacccatg aagattacta ctgcatcatg ataatctaac aatggagata
    64981 cattattttc tttgatgact tctccacctt ttttgatttt cgctccaagg tcttcaatct
    65041 taacgtttcc ttgttcttcc cgagccgaac caaaaatatc gcataagtaa actttatcag
    65101 ctaaatcaag tgcttctgca aattcatcca tcaatgcaat cgtacgcgta aacgtatggg
    65161 gttggaaaac tgcaattatt tctttatctg gatatttttg gcgtgcgcca tcaatcgttg
    65221 ctttgatctc tgcagggtga tgcgcataat catcgactac ggtcatgtct gctacgatct
    65281 tttcactgaa tcgacgtttt actcctggga aagtcagcat ttcttctgca acttctttca
    65341 gatccagttt ttcgaaataa gctactgcaa taacacctaa agcatttaag atattgtgtt
    65401 taccgaatgc tggtacagtg aagtgcccaa caaattcatc cccatgatag acatcaaaag
    65461 cagaaccaga agttgtgcgt tcgatgtttc gtgcttggat atcatcgttt tctgtgacac
    65521 cataataata aatcggcaca tttgctttta atttgcgtaa ataagcatcg tcaccatacg
    65581 caaaaatagc ttttttcact tgtccagcca ttgtttggaa agctgtaaat acatcgtcga
    65641 tacttgtata ataatccgga tgatcaaaat cgatattggt catgattgca tagtctggtg
    65701 aataggcaag gaaatgacgg cgatattcac aggcctcaaa tgaaaagaat tccgcttgcg
    65761 gatcaccatg tcctgttcca tctccaatta gataacttgt tggacggaca ccactcaata
    65821 catgagagag cagtccagtc gtacttgttt ttccgtgaga gcctgttaca gcgatactcg
    65881 tataattttg gataaaatga ccaataaagt cgtgatagcg aatcacttcc aaacctaatt
    65941 cttttgctcg ctggatttct tcatgtgaat caggaaatgc atttcctgca atgatcgtca
    66001 tccctggttt tacattatct gcgttaaatg gcagaatcgt gatatttgct ttttctaaat
    66061 ctctttgtgt aaagaaatat ttttcaatgt ctgatccttg gacatttaga ccttgttcat
    66121 gtaaaacaag agccaaggag ctcattcctg aacctttgat accaacaaaa tgatacaatt
    66181 tattttgatt ttccatttga atcctcttct cttatctagc gtccattctt ttagctatta
    66241 cattcaacac gctcctcatt atcttataaa cgattgaaaa tttcaaccaa atttatttgg
    66301 aaaggaggat tcgcttgcta aaatggtcaa gtacgagaaa aatccgtttt tattgactct
    66361 ttaattctgg tgtaagaaga ccaaacttcc tctgatatat caatgtgagg aataaaatct
    66421 tttcctttcg gaccatagcc gttctcatcg tcatggagac gttccatttc tcctaatact
    66481 agcgcatgga aatattcctt aggccattga tgattcagtt cttttgctgt aaagatgatc
    66541 tcttcagaaa agtgaaggat ttccggcaca aatggcacag cattcgcgaa aatagtttgt
    66601 tcattttgcc atactttctc aaaagttgct cgagtcctgc gttgcagtgt cggatcattc
    66661 atcaataaga ttttttctgg tacagctcct aaggaatgaa gaatctccag tgaaaaagtg
    66721 gcgttctctc cagaatttgt agacttcgat tctatcagaa aagccttatc gggcaaatca
    66781 tagacttctt ttagatactg acgacagatt tcactttcac tcatgccatc ttcaaaatga
    66841 aatccttgct tttcaaaatt ctcataaagg attctagtcg catgccccac ccctccgacc
    66901 aaaaacaatt gatctacttg gccatttaaa tacatttgtg ctgctttatc agctaagata
    66961 ggaagacagt ttccagctaa aataagtgta ggtgccgtca gttcctcaaa atttccctct
    67021 tcaggtgcgg ctaaaaaatt taatagctga ttccagtctt ttagattcct ctgctcactt
    67081 ttttccaaag gccttctatc ctttcatctg attttttgtg cttcattaaa gtcttctctt
    67141 tttgtctcaa tccatttgat ttctccatga catcttccac aacgatattt gcgtacatcg
    67201 attcttctct tgcggcagat cgtggcagaa caatcttggc atcgataata ctccatcttt
    67261 atctgtttct tcgtcaaggc tggtgcatag cgcaaccctc ctgttttttt caaaagatac
    67321 ttgaaatcca tatctttatg ctgataccct cttccagcta gatgaagatg ataatggcaa
    67381 agttcatgtt tgatgattcc tgcaatcgtc tcttcgtctg actcttcaaa tagcttcgca
    67441 ttaaaatcta aatgatgatc gtttaaatga tagcgcccgc cagttgtacg taagcgtgga
    67501 ttgaacattg cttggtgctt gaaaggaagc tggaagcttt ttaatgatat ctcttctact
    67561 aactgctgca gttcttcttg tgtcaatttt tgttactcct cattgttact cttcataagg
    67621 ggaaagcatc gtcaaactga ttctgccttt tttcgtatcg acttgttcta tccaaactgt
    67681 tacgatatct cctactgaca cgacatctgt aggatgcttg acgaattttt tgctgagttt
    67741 cgatatatgg acaagtccgt cttgcttcac accgatatca acaaatgcgc cgaagtcaat
    67801 gacattacgg accgttccag tcaattccat ccctggtttc aagtcttcca tactcaatac
    67861 atctgtacgt aataagggcg ctggcatctc atcacgcata tctcgtccag gttgtgtcag
    67921 cccagctaaa atatcggcta acgtttcttc tccgactgat aggctttcag ccagtttctc
    67981 tacggataat cgtgtcaatt tttctactgc ttcttctgta ccaagctctt tttctgaaag
    68041 ttggactgaa gttaatattt cttttgcaat agaataactt tcgggatgga tacctgtgtt
    68101 gtccaaaatg tttttcccac cagggactcg caagaatccg attgcttgtt catatgcttt
    68161 tggtcctaag cgtggcactt ttttcaattg agttcgtgcg gtgaattctc cgttttcttc
    68221 acgataaatg acaatgtttt gggcagttgt tttattcaat cctgatatat gctgcaagag
    68281 ctgcgggctt gctgtattca catccacacc tacttggtta accgctgttt ccacaacaaa
    68341 atccaattgt tcagccaaac gtttttgaga aacgtcatgt tggtactggc ctactccgac
    68401 agctttagga tcgattttga ctaattcagc taaaggatct tgcaggcgac gggcaataga
    68461 aacagcactt ctttcttcaa cttgtagatg cggaaattct tttcgtgcaa tttcgctagc
    68521 ggagtaaaca gaagcgcctg cttcgttgac gatagcgtaa taggttttat ggtcggcgga
    68581 tttcaactgc tcagccacaa aaagttcaga ttcccgactg gctgtaccat ttccgatagc
    68641 taccatatct acttgatatt tattaattaa ttggataaaa gctggtccag cagcttctct
    68701 ttttgcttgc gctgcaggtt tatgaggata aataacttcg attgctaaaa ctttccctgt
    68761 cgcatctacg actgctagtt tacatccagt acgataagcc ggatcaaatc ccaggacaac
    68821 tttacctttt agtggcggtt gcaacaaaag attacggagg ttttcgccga aaatagcgat
    68881 tgcttgctca tctgcctttt cagtcaattc attgcgcagt tctcgctcga ttgctggctg
    68941 aataaagcgt ttataactat cctgataggc ttctctgacg aatgaagaag aaggagaggc
    69001 aggatttttg actatttggc gatccaagta agctaaaatt gctgcttcat ctacttgaaa
    69061 gaatactttc agaatatctt ctttttctcc acgattagta gctaatattc gatgagaaac
    69121 cattttatgg acgggttctg caaagtcata atacatttca tatacgcctt tttcatctgc
    69181 ttgctcatcc ttgacattgc tgacatacat acctttgtta tacgtataag accgaatcca
    69241 agtccgaaat tttgcattat cgctgacttg ttcagcaata atctcatgag caccgtgcaa
    69301 cgcttcttcg gcagaagaga cttctttttc tttgtcaata tatttttctg cttcagattg
    69361 cacctctcca tctgtaagct gcatcagcca cttcgcgagt ggttcaagtc ctttttcttt
    69421 agcaatcgtt gcttttgtcc gtcgtttttg tttgtatgga cgatacaaat cttccacttg
    69481 ttgcattttc acggcttggg tgatttctgc ttctaactcg ggagtcagtt tcccttgttc
    69541 atcgattagc cgaatcactt cagttttacg tttttctaag ttttcaagat aggcataccg
    69601 ctcttcgatt tcacgaatct ggacttcatc aagacttcca gtcatttctt ttcggtaacg
    69661 cgcgataaaa ggaacagtgt ttccttcatt taacaaatta agtacagtag tcaactgctt
    69721 aggacgatag tctgaaagtt ctttttgtac aagctgaatg atcgtctggt tcaaattttc
    69781 cgtcattaaa atgcctcttt tctgctaaaa attgcttctt tattctatca tactttttat
    69841 cctgggttaa actactgaaa gaaacaatat tctctataaa aataaaaaga gccgcaacaa
    69901 agtcacagct ccttcattct ttccattagt cagattccat gccaaggtcc ttcgttgttc
    69961 tgatattcaa tatcttgaag aataccttcc ttgtcaaaaa caaccccatg caatgaccct
    70021 ccgaatacag ccccaccgtc cattccgatt ttatgatcct gaatccagag gtctgttgtc
    70081 tgcatatctc catggagcat tggtgtaatc gtatgtccaa atacaattgt cttacctgta
    70141 ttgtttttcc cttcatgaaa tggttccctt atccaaataa agtctcttag acttgtctta
    70201 cgccagtctt tctttgtcaa atccacaccg gcatggacaa aaagataatt ttcccattca
    70261 taatacaagg ggcgctggac taaaaattta atcagttctg aatatctcga ttggatcatc
    70321 atcgagattt cagttgggga gtattcctcc gatgcccctg ggtgaatcag gctttcaaac
    70381 gtttcttttc caccatttct catataaggc gcataccagt cctccggtga ttgcagaaat
    70441 tgcaaaaaat attcttcatg gtttccccgg aggtaaatgg ctcctgtttc ttcgactaat
    70501 tgctttccta ataaaaaaca ttcttttgta cgaggacctc gatcattgag atcccctaat
    70561 aaaacaagct gatggatctt tggatcataa tagtgaataa gtttttcaaa aagctcatgt
    70621 ttgccatgga catccccaat agcataaatc aatgatttca tgtggatcac catcctcaac
    70681 gctagtatag cgcatattgg aaatggtcga caatcaaaac tattgttttc tatttaatta
    70741 aagctgtaaa ccgcagttct gcttcttctg gagagattcc atggtcttga acatagcgat
    70801 tgcgcggatg tttcgcacac tctatgctac atgctcctaa atgtttttct tcattttcaa
    70861 tggaagcaag aatctgacga ttgcactcag gattgctaca attgatatat cgttcacatg
    70921 gtgttccgtc aaaccagtcg cgaccgacga tttgtttatc tacttggttg atatcgacag
    70981 cgatccgttc atcaaacaca tacatcttgc cgtcccataa ttctccttgg acttcaggat
    71041 ccttaccata agtagcaata ccgccatgca gctgtccgac atctttgaat ccttcacgga
    71101 caagccaacc agaaaacttc tcgcaacgaa tgccaccagt acagtaggtc actacccgtt
    71161 tttccataaa ttcttctttg tgctcgcgga tccagtctgg aagctctcga aaactgcgta
    71221 tatctgggcg tacagcccct cgaaaatgtc ccaagtcata ttcataatca ttccgtgcat
    71281 caatcaccac tgtctgttca tctaaaatag cttctttaaa ttcttgcggt gaaagatagg
    71341 cacctgtcac ttggtttgga tcaatgtcct cttctagctt taaacttaca agctctggac
    71401 ggtatcttac aaacatcttc ttaaaggcat gtccttcgct tggatcgatc ttgaacatcg
    71461 tatcagcaaa tcgaggatca gctttcattg ctttcatata ggcagtcgtg ttttcaacgg
    71521 ttccagacac agtaccattt attccttctt cagctactag aatacgacct ttaagatcca
    71581 aagatttgca aaaggctaaa tgatttttgg caaattcctc tggttcttcg atttttgtat
    71641 aattgtaata aagcagtact tgataatcca tgtatttctt ccttccttct tttcgttcct
    71701 aaacagtata aagaaggcgt tcgcatttga cgatttattt gcttgtgtat aattacgcat
    71761 ggaaagtttt ttgtttacat aatattatta tttgaattaa aaagacttcg ctttcctgtt
    71821 tcgcacagaa aaacaaagtc tttatttcta ttatttacct gtaaccgcaa agtagttgcc
    71881 atcgggatca gcaaagttaa agacatattc ttctccaagc tgtaccatat cacctaatgt
    71941 gactgattgc gtctgcattt ttttatatag tccaaagata tctttactgt aaaacatcaa
    72001 agatggggca ctagtagctg tgccaggtga atgggactca acaaatgctc gatcatataa
    72061 cagaaatgag gtttgactgt cctcaactcc tacttcaatt ataagcgtcc catccatttc
    72121 ttgacgatct ttttccgcaa agccaactgc tctccaaaac gttgcatttt tttctacatc
    72181 gtccacataa agcatgattt ttatttcatt cgtaaacatc t
//
LOCUS       DAYYNQ010000004        67690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000004 DAYYNQ010000000
VERSION     DAYYNQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.004"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2206)
                     /locus_tag="ACHBJ1_000227"
     CDS             complement(<1..2206)
                     /locus_tag="ACHBJ1_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429202.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNE"
     gene            complement(2329..3879)
                     /locus_tag="ACHBJ1_000228"
     CDS             complement(2329..3879)
                     /locus_tag="ACHBJ1_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9429203.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3881..4297)
                     /locus_tag="ACHBJ1_000229"
     CDS             complement(3881..4297)
                     /locus_tag="ACHBJ1_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429204.1"
                     /translation="MNNTIPYGYVIKNGEINVDEQQAQQIREFFNIYLEGVGLQEAGK
                     RSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVP
                     DTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            complement(4294..5847)
                     /locus_tag="ACHBJ1_000230"
     CDS             complement(4294..5847)
                     /locus_tag="ACHBJ1_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9429205.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5908..6081)
                     /locus_tag="ACHBJ1_000231"
     CDS             complement(5908..6081)
                     /locus_tag="ACHBJ1_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM9429206.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6376..6681
                     /locus_tag="ACHBJ1_000232"
     CDS             6376..6681
                     /locus_tag="ACHBJ1_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429207.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6742..7779)
                     /locus_tag="ACHBJ1_000233"
     CDS             complement(6742..7779)
                     /locus_tag="ACHBJ1_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9429208.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7895..8803)
                     /locus_tag="ACHBJ1_000234"
     CDS             complement(7895..8803)
                     /locus_tag="ACHBJ1_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9429209.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8793..9212)
                     /locus_tag="ACHBJ1_000235"
     CDS             complement(8793..9212)
                     /locus_tag="ACHBJ1_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM9429210.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9213..9620)
                     /locus_tag="ACHBJ1_000236"
     CDS             complement(9213..9620)
                     /locus_tag="ACHBJ1_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM9429211.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9607..9774)
                     /locus_tag="ACHBJ1_000237"
     CDS             complement(9607..9774)
                     /locus_tag="ACHBJ1_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429212.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9784..13674)
                     /locus_tag="ACHBJ1_000238"
     CDS             complement(9784..13674)
                     /locus_tag="ACHBJ1_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9429213.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13671..14363)
                     /locus_tag="ACHBJ1_000239"
     CDS             complement(13671..14363)
                     /locus_tag="ACHBJ1_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9429214.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14440..14841)
                     /locus_tag="ACHBJ1_000240"
     CDS             complement(14440..14841)
                     /locus_tag="ACHBJ1_000240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429215.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14912..17473)
                     /locus_tag="ACHBJ1_000241"
     CDS             complement(14912..17473)
                     /locus_tag="ACHBJ1_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9429216.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            complement(17680..18060)
                     /locus_tag="ACHBJ1_000242"
     CDS             complement(17680..18060)
                     /locus_tag="ACHBJ1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429217.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(18070..18660)
                     /locus_tag="ACHBJ1_000243"
     CDS             complement(18070..18660)
                     /locus_tag="ACHBJ1_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9429218.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18663..19004)
                     /locus_tag="ACHBJ1_000244"
     CDS             complement(18663..19004)
                     /locus_tag="ACHBJ1_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429219.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(19001..19432)
                     /locus_tag="ACHBJ1_000245"
     CDS             complement(19001..19432)
                     /locus_tag="ACHBJ1_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9429220.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19425..19763)
                     /locus_tag="ACHBJ1_000246"
     CDS             complement(19425..19763)
                     /locus_tag="ACHBJ1_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9429221.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19760..20071)
                     /locus_tag="ACHBJ1_000247"
     CDS             complement(19760..20071)
                     /locus_tag="ACHBJ1_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9429222.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(20082..21293)
                     /locus_tag="ACHBJ1_000248"
     CDS             complement(20082..21293)
                     /locus_tag="ACHBJ1_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9429223.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21297..22052)
                     /locus_tag="ACHBJ1_000249"
     CDS             complement(21297..22052)
                     /locus_tag="ACHBJ1_000249"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9429224.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21952..23280)
                     /locus_tag="ACHBJ1_000250"
     CDS             complement(21952..23280)
                     /locus_tag="ACHBJ1_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9429225.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23346..24080)
                     /locus_tag="ACHBJ1_000251"
     CDS             complement(23346..24080)
                     /locus_tag="ACHBJ1_000251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429226.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24136..25701)
                     /locus_tag="ACHBJ1_000252"
     CDS             complement(24136..25701)
                     /locus_tag="ACHBJ1_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFM9429227.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVFPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25784..25927)
                     /locus_tag="ACHBJ1_000253"
     CDS             complement(25784..25927)
                     /locus_tag="ACHBJ1_000253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429228.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25924..26100)
                     /locus_tag="ACHBJ1_000254"
     CDS             complement(25924..26100)
                     /locus_tag="ACHBJ1_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM9429229.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(26084..26278)
                     /locus_tag="ACHBJ1_000255"
     CDS             complement(26084..26278)
                     /locus_tag="ACHBJ1_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429230.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26280..26657)
                     /locus_tag="ACHBJ1_000256"
     CDS             complement(26280..26657)
                     /locus_tag="ACHBJ1_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM9429231.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26731..27966)
                     /locus_tag="ACHBJ1_000257"
     CDS             complement(26731..27966)
                     /locus_tag="ACHBJ1_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM9429232.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27966..28748)
                     /locus_tag="ACHBJ1_000258"
     CDS             complement(27966..28748)
                     /locus_tag="ACHBJ1_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM9429233.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28763..29335)
                     /locus_tag="ACHBJ1_000259"
     CDS             complement(28763..29335)
                     /locus_tag="ACHBJ1_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9429234.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29469..29834)
                     /locus_tag="ACHBJ1_000260"
     CDS             complement(29469..29834)
                     /locus_tag="ACHBJ1_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9429235.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(30002..30430)
                     /locus_tag="ACHBJ1_000261"
     CDS             complement(30002..30430)
                     /locus_tag="ACHBJ1_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM9429236.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30427..31779)
                     /locus_tag="ACHBJ1_000262"
     CDS             complement(30427..31779)
                     /locus_tag="ACHBJ1_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM9429237.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31779..32063)
                     /locus_tag="ACHBJ1_000263"
     CDS             complement(31779..32063)
                     /locus_tag="ACHBJ1_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM9429238.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(32060..32269)
                     /locus_tag="ACHBJ1_000264"
     CDS             complement(32060..32269)
                     /locus_tag="ACHBJ1_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429239.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32407..34650)
                     /locus_tag="ACHBJ1_000265"
     CDS             complement(32407..34650)
                     /locus_tag="ACHBJ1_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM9429240.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34643..34987)
                     /locus_tag="ACHBJ1_000266"
     CDS             complement(34643..34987)
                     /locus_tag="ACHBJ1_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM9429241.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34980..35750)
                     /locus_tag="ACHBJ1_000267"
     CDS             complement(34980..35750)
                     /locus_tag="ACHBJ1_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9429242.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35961..37898)
                     /locus_tag="ACHBJ1_000268"
     CDS             complement(35961..37898)
                     /locus_tag="ACHBJ1_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM9429243.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(37959..38501)
                     /locus_tag="ACHBJ1_000269"
     CDS             complement(37959..38501)
                     /locus_tag="ACHBJ1_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM9429244.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38503..39645)
                     /locus_tag="ACHBJ1_000270"
     CDS             complement(38503..39645)
                     /locus_tag="ACHBJ1_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM9429245.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39646..39960)
                     /locus_tag="ACHBJ1_000271"
     CDS             complement(39646..39960)
                     /locus_tag="ACHBJ1_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM9429246.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39965..40171)
                     /locus_tag="ACHBJ1_000272"
     CDS             complement(39965..40171)
                     /locus_tag="ACHBJ1_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429247.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40268..40885)
                     /locus_tag="ACHBJ1_000273"
     CDS             complement(40268..40885)
                     /locus_tag="ACHBJ1_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429248.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41280..42230
                     /locus_tag="ACHBJ1_000274"
     CDS             41280..42230
                     /locus_tag="ACHBJ1_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM9429249.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42223..42669
                     /locus_tag="ACHBJ1_000275"
     CDS             42223..42669
                     /locus_tag="ACHBJ1_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM9429250.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42672..44504
                     /locus_tag="ACHBJ1_000276"
     CDS             42672..44504
                     /locus_tag="ACHBJ1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9429251.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44520..44723
                     /locus_tag="ACHBJ1_000277"
     CDS             44520..44723
                     /locus_tag="ACHBJ1_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429252.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44774..45910)
                     /locus_tag="ACHBJ1_000278"
     CDS             complement(44774..45910)
                     /locus_tag="ACHBJ1_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9429253.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45900..47855)
                     /locus_tag="ACHBJ1_000279"
     CDS             complement(45900..47855)
                     /locus_tag="ACHBJ1_000279"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9429254.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47848..50565)
                     /locus_tag="ACHBJ1_000280"
     CDS             complement(47848..50565)
                     /locus_tag="ACHBJ1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM9429255.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(50566..50787)
                     /locus_tag="ACHBJ1_000281"
     CDS             complement(50566..50787)
                     /locus_tag="ACHBJ1_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429256.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(50936..51772)
                     /locus_tag="ACHBJ1_000282"
     CDS             complement(50936..51772)
                     /locus_tag="ACHBJ1_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9429257.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(51981..53357)
                     /gene="rlmD"
                     /locus_tag="ACHBJ1_000283"
     CDS             complement(51981..53357)
                     /gene="rlmD"
                     /locus_tag="ACHBJ1_000283"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9429258.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(53468..54523)
                     /locus_tag="ACHBJ1_000284"
     CDS             complement(53468..54523)
                     /locus_tag="ACHBJ1_000284"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9429259.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(54533..55963)
                     /gene="gatB"
                     /locus_tag="ACHBJ1_000285"
     CDS             complement(54533..55963)
                     /gene="gatB"
                     /locus_tag="ACHBJ1_000285"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM9429260.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(55960..57432)
                     /gene="gatA"
                     /locus_tag="ACHBJ1_000286"
     CDS             complement(55960..57432)
                     /gene="gatA"
                     /locus_tag="ACHBJ1_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM9429261.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(57432..57737)
                     /gene="gatC"
                     /locus_tag="ACHBJ1_000287"
     CDS             complement(57432..57737)
                     /gene="gatC"
                     /locus_tag="ACHBJ1_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM9429262.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(57843..59879)
                     /gene="ligA"
                     /locus_tag="ACHBJ1_000288"
     CDS             complement(57843..59879)
                     /gene="ligA"
                     /locus_tag="ACHBJ1_000288"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM9429263.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(59896..62130)
                     /gene="pcrA"
                     /locus_tag="ACHBJ1_000289"
     CDS             complement(59896..62130)
                     /gene="pcrA"
                     /locus_tag="ACHBJ1_000289"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM9429264.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            62285..63844
                     /locus_tag="ACHBJ1_000290"
     CDS             62285..63844
                     /locus_tag="ACHBJ1_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM9429265.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(63955..65886)
                     /locus_tag="ACHBJ1_000291"
     CDS             complement(63955..65886)
                     /locus_tag="ACHBJ1_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM9429266.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(65904..66818)
                     /gene="pfkB"
                     /locus_tag="ACHBJ1_000292"
     CDS             complement(65904..66818)
                     /gene="pfkB"
                     /locus_tag="ACHBJ1_000292"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM9429267.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(66815..67567)
                     /locus_tag="ACHBJ1_000293"
     CDS             complement(66815..67567)
                     /locus_tag="ACHBJ1_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9429268.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT        18466 a        15283 c        12183 g        21758 t
ORIGIN      
        1 tttcattctt atatttgtta taaataaact ttttaaatcc aacatcattt tttaaagaat
       61 cgttatcata aatcttagga tatttggaaa caccacatcg aagaataaat gacagaaaat
      121 ctctctcctc agcaaaacta gagcaatcta tttttttcaa tgccgtgtaa tctgtatctt
      181 gaaaaagctt atctcctaat tcattgccat actcttttcc aatatacatt ttattggaag
      241 gttgaacgtc accagcaacg tttggaagat taaatggaac tttagacagc tcacttccag
      301 ctgatagttt ttcattgtag ttatcataaa accaattaat gaaactgata gatttttcaa
      361 cagtgtcaat ttgtcgatta atcgttccaa tgatcaaatc tgaactgctt tgctcagtga
      421 attcaattgc aagatatttt tcactgaatg catctagaat acgtttatct cctgtacgtt
      481 cagctttgta tgcattagct gtttcttccc atttattttt ccaacttgat gaagtcttaa
      541 tctgataaat taactcatca acatatgttt gtttcaaaat gcttaaacta acccaagaag
      601 acagctcttt tggaagcaca gtaacacctg tatcggtcgg taagaatacc ttatctgatt
      661 tcatcaacca attgtcacta ttgtccttta atagcttagg gacaatcgga gaacttttat
      721 attgctcact ccaccataag aacacttttg tccttattct attttcccaa gaagatgaaa
      781 gaatgttgat tttatcagat agttcatcta caacaaacga caggccagtg tattcattat
      841 aattagcaag ttcttgtact agttggtcat tttcgtttgt ttctaataat tgcaacaagg
      901 tggaaaatgt ctcacctatt agctcctcag ggaattcttg aaggaaaagt tttggattat
      961 ctttgataga aatatattgc caattgacag tcggtagtat ttgggcatta gataaaatac
     1021 ccaagaattc gttcattaag tcaaatacat ctaagtccca tagtttattc tctttaaaag
     1081 gagttaccat ttttaatgct atgtcttcat attcaggctt agataatttt tctgctactt
     1141 cagaaataaa tttgagcacc tcaataaata ttttcttatt tatttgttca ctatcatccg
     1201 ggacgtcatc acgtgaatta tttgttataa acggtgcatt tataagtgca tgaattggtg
     1261 taccaaaatt acgtactgga aagtagcaat atagtttttc tttggagtaa tcgtaatcag
     1321 aatctttaga aacggctagt gcaattttag caactttatc tcctatcata gattctttgt
     1381 caaaaacaaa atattcttca gacggtgatt gaccagtaac atcacttttt tctatcaata
     1441 catcatttcc atccacaatt ttctctgata ttttttcatc atcagttgtt tcaataacaa
     1501 tttttgttat atttggtaaa aataacagtg acttataaaa tggctgctga agttgtttag
     1561 aaacactcgt gcctttactt aaattagttt cttttagttt tactcgaatt aatgtatcaa
     1621 aaccagaatc aattttgttt attggttgag gacagttcat tattggaaaa cataaatcat
     1681 aatcttcttt tttccaattt ttcttctgct caaaaattaa ttcactgaat ttgtcagaat
     1741 ctcttgatac atactgctca aaaagctttt ttgtgaattc ttcactaaat cgaatagcaa
     1801 attctcccga atacagttcg acccactcag catcgtttag caaggaacga aaaccagtcc
     1861 ccttatttcc gatattttgt gatgatttat tactcgctct tcctaagcac aatctttcaa
     1921 ttgtatcttt tgaaaatgaa gtgcctgtat tcccaacttc caagatatta tttttatatg
     1981 ttatctgaac tttcacttcg tcattgctag catcattttc tgattttata tctccatagg
     2041 catcgtctac attctgtaat aactctaaaa tctgacgccc attataatca cgcatcaatt
     2101 catattcttc gctgcaggcc ttttcaagtt cattactcaa ctcagaatcc gtatatttac
     2161 gaataaaaat atcccttaat tcatcaatgg tattccattg gctcacagaa cttcgccccc
     2221 agcctatttt ttacataata tattatatca tcaatcactt tatgaaatcc aatcaataaa
     2281 gacgttaata tggctaccca cattaacgtc tagtcctatc tattcaaatt aaatttctac
     2341 agtcactctc accccacttt tgaaatcaac aacaatgttc ttttcaaaaa tggtgacctt
     2401 ctcaatcaac cttcgaacaa gcacttcatt atactcattg atgacatcgt gttcttttac
     2461 aaagactacc atctcatcaa cccgttgttg caaattagta ttcgctgcta tttgttttgc
     2521 aaccgcatct cgttcatctc ttagtgaaaa tatttggctt gccaattcat cgtaaccctg
     2581 accgccaact gccaggctaa taatttcagc ttcaagctct ttcaactgac tgtctagttc
     2641 tgccaatcgg tcagttaatc catcagtcat aactgagttg atattgtcat ttaattgctg
     2701 aagaaaagca tcacgatcga ttaacgtttc attgattgcc ttgataacag ctccatgcag
     2761 gtctgtttcg tagattgttc gacccgtgca attatgttct cgtttatcca ctctggattt
     2821 gcatcgccac acagttcctc gattcttcca atgaactctg cggtaagcat tgccacagtc
     2881 accgcaaact gtgataccag ataacgcata cttcccagaa aagcactgtg actttttgtt
     2941 tttcattgat ttacgccggt ctagttcttg ctgggcttgc atgaagatat cttttgggat
     3001 aatggccgga tggctatctt caacataata ctgctgagcg taactcccgt cattcttcac
     3061 tcgtttcttt gtcaagaaat cggtagtata tgttttttga agcagcgcat cccccatgta
     3121 cttctcgttt cgaagaatca atctgactcc ttctcctcgc cagagctttc gcttccctcc
     3181 cgttaggatg ccatcagcca ttagaccatt agcaatttct tgcaaggaac tgccctcaag
     3241 gtattcgcgg aagatgcgtt tgatgacttc tgcttccttt ggttcaatca ccaagttacc
     3301 atcttcgtcc ttagtgtacc caagaaaatg attgtggttg atacgtagct gtccctgctg
     3361 gaaacgatac tgtacgccca tcttggtgtt ttgacttagt gactggcttt cttgctgggc
     3421 cagacttgcc ataatggtca aaagtacctc acccttcgca tccattgtat tgatgttttc
     3481 cttttcaaaa aacactgcaa tcttcagttc ttttaactgt cgtatgtatt gcaagcaatc
     3541 aaccgtatta cgggcaaagc gtgaaattga ttttgtgatg atgtactcaa cttcgccgtt
     3601 ttgacacgcc tcaatcatcc ggttaaaacc gtcacgcttt ttcgtcccag ttcctgaaat
     3661 cccttcatcc gcaaatattc ctacaagctc ccaatcggga tttctttgaa tgtagtccgt
     3721 atagtgttga acctgtgctt cgtaagaact cgcctgttcc tccatttcag tagaaacacg
     3781 acaataggct gccactttca actttggttt ctcttcggta ttctctgctg cccattgtct
     3841 aggcttcatt ggaataaccg tcacctgttt tcctgccatt tcacttaacc tccttgattt
     3901 ttgaatatgc atattgcgct tgtttgaatg ggtcagaata cttttcggta atcttgccag
     3961 atttaaaatg agtgggtatt accttcgtat ctggcacttt gatctccttg cgttttttaa
     4021 agaacttaat tcttcgttct tgctccagat ccgcttggaa gaaaatatcc tcatcaatga
     4081 tttttggata tatctcattt cctaagtaaa cctcattcat caaaatacgt ttcataaagg
     4141 gatgcggctt gttaatgcca gagcgtttgc cagcttcttg taagccaaca ccctcaaggt
     4201 aaatattgaa gaactcacgt atttgttgag cttgctgttc atccacgttg atttctccat
     4261 tcttaatgac atacccgtat ggaattgtat tgttcattct ggtaacctct ctttcaaaag
     4321 tagtccgcac ttaagttcaa atccgacttc ttggcggttg taaactgtaa tgctgtttgt
     4381 gaacttcttg aataaatctt catcccagtc cgtcaacatc tctgccatcc gcgcgaattt
     4441 gtataaggct tctagttcaa tttgctgagt gcttttgccg ttgactgact caatgatgag
     4501 gcgctgcttt tcttgtgcga cagccctctc ttgttgaagt ttgctttgtt cttctaaaga
     4561 caccttcgtt gataaaatac tggaagcagt caaacttgcc agaacctcaa gcttttcttg
     4621 aatcttgtcg atttcatcag caagcacttc gagcccttcc tgattcgcac ctgctttatt
     4681 caaagacttc ataaaaggtt ccagaaccaa cttgtgtccg aaaatcaact tattcaacat
     4741 ggtcaaataa gcaaccttca aatcttcatc cgtaattggt aatgcatcgc agctatcttt
     4801 atttttaaga tgccccgtac acttccactg aaccttcttc gtattacctt tccaataagt
     4861 gtgaacccgc ttgaatttgc tgccgcattt atcacagatg atgttgcctg aaaatgcgta
     4921 tcgcttggtg tactttttcc cgtcaccagt attccctttg gcctcagctt ttctccgaat
     4981 gagttcttga actcggtcgt aatcttcttt agatactatg ggttcgtgat tgtttttgat
     5041 ctggtacatt ggcaattcgc ccttgtttat gcgtcgtttg tactggtcat caacaaaggt
     5101 ttgttgcatc gtcaaagtcc cggtataaac ttcatttttc aaaatacttc tgactgatgt
     5161 tgcttgccaa tttgccccac gtttagtgaa ttgttttcca tcgtttaacc gcttggcaat
     5221 cattccagtc gaaatgccat ctaatgccca ctggaacatt tgtttaacaa cttttgcttc
     5281 agctgcaacg atgtgaaaat catagccttt ccgcttgtag ccatacggcg ggtaagcttt
     5341 gaatttccct ttagacatcc ggtttcgaat cgcccacttc aagttttctg aatgggaata
     5401 ggattcactt tctgccaagc ttgataaaat tgaaagcaac aactcgcttt ccatatcacc
     5461 agtattaatg ttttcctttt caaaaaagat ggtgacacca atatcagtta gctgccgaac
     5521 cagattcaag cagtcaactg tatttcttga aaatctggaa atagactttg tcagaatcaa
     5581 atcaatcttt ccaagctctg catccttaat caatcgtttt aaaccatcac gttttacagt
     5641 cttggttcca gaaatccctt catcataata aacaccgaca tagtcccatt gttcatggtt
     5701 cttgatgtga ttttcatagt gagtaatctg ggtttgtaaa cttgccagct gttcattact
     5761 tgatgttgat acacgagcgt atgctgccac ctttagtttg gaagcttccg gcaactctgg
     5821 acgtgccaat ttatgtatcg ttttcatcag ctcaactcct ttccattagg tgacatgtta
     5881 gctctaaagt gccgttatat caacgtttca gaccattatc cttgataaaa aaggattgaa
     5941 tgtttcccgg tttttattgt cgattttgac gtattcttcc aggttgattt taccggtaac
     6001 aagtaagttc agaaggatct gctgtgaaat gtaataggct gcttcatctg aatagttaat
     6061 ttctgttttc ttgttagtca ttgctttcgc ctcctcaaga tacaggcgaa gaaaatccat
     6121 gaaagtaaac ccaatcacaa attttccttt gcctttcatc catatgcgaa agaaataacc
     6181 gactcgaacc cacaaaacga aaaaagcctg caagaatgaa acgaatcact cttgcaggct
     6241 tctaagtttg tttatatata gtaggaatta ttctttgtaa attttgactt tttacttccc
     6301 aaacatcaaa taagccttta caagcttcat ccaataaata acaatataaa tatcaattgt
     6361 gggaaggaga aagttatgag tagttttgat atggaagggt tagaaaaaga atatgaacat
     6421 ttttgcaaat ctgcgacaac aaaggaactg gaggaatttg atgaaagctt caaacaagaa
     6481 tttgaaaagt gctttaaaac catagaggct gaagaagctt tataccagtt tttacaagcc
     6541 aatataaacg cgatgaataa tcgtatggaa tcaatagaat cgcctctttt ctgcatcaat
     6601 tacaaagagc aggtatttca gttaagaaat catttaatcc taatgcaaaa tgctgtaaac
     6661 acctacctta aagaaaacta gacgaaaaaa aggccccaca ataaaacttg tgaggctgtt
     6721 ttgaattata aagacgtcaa actacttatt gaaaattttg tagctcgttg gaaccatcgt
     6781 gaagttttct tcagtaattg ggccatcata aattttgatt ttgcaaactt ttgacgccac
     6841 atcaaggtcg tgaacaaagt aaattttata tggtgtatct ccaagggagg cagcaacctc
     6901 attagtactc atttcaaatc cgtcagagaa gtttgttttt gaagctttca cttcaatatg
     6961 gatttcatta cctgattggt caaacgcaca aatatcatag ccatgaccat ctccttcagt
     7021 tttagaaaca tgttcaggaa gttttgtttt gttcttttct gcttcctgaa tgaggaaatc
     7081 aagtacgatt tcttcgccta gagcacccgt tttttgtttt gccttgttga cacgctccca
     7141 atcagtatgg cgtccagtga attttcgttt ctgagtccca ttcgtgcgat ttggtcccac
     7201 ttcatcaaca agaacagtaa atccttttga actttcttgc tcaatttctt caacttcctc
     7261 gtttgtaaga tccagttcag acggcaacga ttgtgtatca tcgattggga taagatgacg
     7321 attttcaatc aatgcaacaa aatcggcata ggtaatttca ttcattccat actgatccca
     7381 gaagtaggcc catgaattcg gctttttacg cttctttttg ctaggtgtat tccaatcgaa
     7441 gttttcaata aatgaatcac cttgtttaat tgccggttcg attgtaaatg cgtgtgtaat
     7501 tactgccgta acatttccag cttcatcagg ttcagaaatg ctctcaacgt atccgccccc
     7561 gtaaatttcc cacttacggt ctttagtaat tttccccgga tgacgattca aaacaaccgc
     7621 gccggctttc aatttaccat attggcgcat gttccaaccg taacttttag catcatcagc
     7681 gtatttcaac tgaccatcat cttctgtttt cccgtgatct aaaatgaaag cttgttggct
     7741 ttctgttggt tctggtaaca aatctttaaa cgtaaccata tcgatacctc tttctataat
     7801 tcaatcggaa caaatttatg ttctggttga attaataata acacgttcga tattttaagt
     7861 caacaatccg gacataattt tgttccgatt aaatttatga aatcaaaata taatcgtcag
     7921 attccgtctt accagtattc ttaccgacag cacaatatcc aatcgggcaa cgagcccata
     7981 cccaaccatc cgcaatcgtc atcttctctg acttcggaag cgtccaagtt gcgcctttat
     8041 tgtatgaacc aacaaccgca ccacttaaac ttggcttgtt gcgaacgttg agaccactca
     8101 cttgaaccac aagagttttc acgttggatg ttgatgaccc agatggataa acttgattac
     8161 cgttctggtc gaacacctta taaccagaat ggctatctgc agcccgcttc gcattgttca
     8221 agtccttata ggcaccaatt tgtgacttgg cgtcagccca tgatttgcga acacgataca
     8281 gaccactagc tggcgtgctt ggggttgatg aaccattatt aatcgcattg gctttatccg
     8341 caatctcctg cagtttacca agcaactgtc ccggacatga tgtggccgaa aaatgtgagt
     8401 gagggaagat attcttgttc accaccaatt tccctagacc ataacgtttg gcaatatctg
     8461 cacacaactt tgcacttgca tcaatagttg cttgattgat accccagttc ggtgatccag
     8521 tgctattgca gtgctcaatc ccgatacttg agatattggc gttccaatta ccagcgtgcc
     8581 aagctgtgtt tccttcgtcc acgtatgctc gaatttctcc attttttcca ataccgtagt
     8641 gtgctgacgc ctcacgcgtt ttccaaatat caggaacaat atcatagttg gtggttgcca
     8701 tgtgatgaat cactatctta tctactttgg caccactacg accattcgag taatggctaa
     8761 atgactgaaa tccatatttt gttgctagat tactaattcc cattttctgt ttcctccttc
     8821 ttttcgtgta actgctgcaa gatttcacgc aacttttccg gcactggcag tccaatcgcc
     8881 acggcattct ccagcagtga aattccttcg tttgagatat agaagaaaat gaccgcatca
     8941 cgcagtacat ttccaccttt gatgacttcc acatcaagca tatgagccac accaaccaaa
     9001 agaaaaatca tgattttctt agtgatgccc tttgccccaa tcttgctgga tagtttcttt
     9061 tcgttgattg cacgaagcag accagttaca tagtccacta ccacaaaagt tacaagcgta
     9121 taaatcaaac catcaattcc tcccacatac catccaatca ctcctccacc acctgcgatg
     9181 gcgagttgaa tccaattcca cacttctttc atttacaaat cctccaattg ttctaaaatt
     9241 aaatcgagca tttcagcatc tttccctgaa atggtgactt cactattttc aaatccagca
     9301 accaataaag gcagcttgtc ttgaagttca tccacgttga tgtgggtttc ttcgttgagc
     9361 aagtctgcct tttctttgtg atattcagtt gcggtttctg ggatgagtga aaaggcaccg
     9421 tcatgttgga ccaaattgcc gtgctcatct ttctttccgt atttttgaag caagtctacc
     9481 tcgtcttgat agagttcatc aaccttcacc tggatgagtt ttgcaagctt cgttcgcact
     9541 cggctaatct ttggtggtaa gtcaaaacct tttaagaaac tcatcacgtc caccaaatct
     9601 tcatttctaa ttttgagcat tatctatctt ctcctttaaa tcaccaatct ggacacaaag
     9661 atcatccacg accatcacaa gcaaatcaac catatccgtc ccaactttca ttggctttgg
     9721 aatatctgcc tcgataggtt cagttactat cacttcttct ttttcttcgt tcatggcggc
     9781 ctcctaactg tatgtggtca ttgaattaaa cgactgcgga ttgtaccaac cactcgctgt
     9841 accgttgcta ctgaaaccgg ttggtaaagc gattgttcgt cccgcaagtt tggcacagat
     9901 ttcacggatg actccaaagt caacccactt cccatcatct ccgagcatca gattagaacc
     9961 gttgaatgca ataccagcgc tgccggaact tgagccaatc ttagtataaa gggaactact
    10021 aaatgtaatc ccaccaacag atatttgatg ttgtgtccct atacgaagca ttgatggtaa
    10081 ataaatgtcg tcgtcaaagt ggaacccctt ctccatacca gactgcaatg ttctcgcata
    10141 ccaagaaagc ttctgggtgt agacactcgc agaggaggaa tctttatgag cccatgacat
    10201 atactctgtt ccatactcaa gatcaaatac caatccacgc caatcagagt gaccactgat
    10261 gttatttgca ccaattgttc cgactgtcgt tccaccacgt cgaatggtca ttccctgcga
    10321 gtttatttca gtcacaagag tgcttcctga ataatatcca atcgtagttc ctgtgattcg
    10381 aatggacgaa ctcgcagtca atgctgttag gatattagca gcaatcttat cagccgtaat
    10441 cgagttcgcg ccaatacggg ctgccgacaa agttcctgtg gtaatcttcg ccgcatcaat
    10501 gcttgcaatt tttgcacttt gaattgtggc atctgcaatc tttgcgtttg tgatggccgc
    10561 attttggatc atggcagaac taatcactgc actatcaatg gttgtttgac cagtgatgtg
    10621 caccttgttt ccagcaatca aaatactttc aggtgagata ttgatttgat taatcacgtc
    10681 tcctttacca actcgtaaat tgattaagtc tttctgcatg agaattgctg aatataatgt
    10741 gttggcattg gtatagtctt gccttgtggt cgtaggataa atatttcctt catattcatc
    10801 gggtttgatg ctcgtgttca ctcggactcg cattttcaat gtgccactcg ggactgtaat
    10861 tgctccgctc gaaccagtat aagagatttg atgataactt gaaccatccg acgggtttag
    10921 agtaaaaggt gtgttgcttg aattataaat cccagcatat gtgccagtct taactgaaaa
    10981 gaaagctaga tacacgctgt cagtagtaat ccctgatatc tgctcagccg ttaacgtcca
    11041 agaataagtg gcattgctgc tacttgtaac ggtcgaaatc tgttcccaag caccacctgt
    11101 ataaagcaag acaatattat ctgatgtatc cttgcgacca ttccacgaaa ttacactcgg
    11161 agcgactttg ccttccaatg gcaatttgta aatgaccaga taatctcctg aatttgaata
    11221 ggggtttgtt gtcacgccac ttgtaatact catcgattca acgttcttac ttactagata
    11281 actcgcaatg gtcgcattag tataagttcc cgccgttagg gtttcactaa attgttttgc
    11341 cgttgggaca aaatcaatgt aaggtaaatc catgccgtag taatgatagt agaaagcagc
    11401 ttgagttccg tcacgcagtt gcccgatata cttatctccc actttgacat caaggaactc
    11461 acttcggaga taattggttg cggacacttc tgaaccgtca gtggtactca aacttccttg
    11521 ttcccattga ttgactttaa atggaacgcc gattgcttct ttggtttctg aaacacttaa
    11581 cagtaaggca tcagtggtcg ctgtaagttt tacgacgtta cttatctcgg ataaatcaac
    11641 atagccattt gtcacatccg ttttcaacat atagcaactg atggtttctg gtgcatcact
    11701 agttgttaaa cgaatgcgaa taaaagcggc actgcttgga acagtaagcg gtgtggtggt
    11761 gtttccaaat tgggtgtacg ataaaaagga cttatcctct ttgtaataaa gcagataagc
    11821 cgttgcgtaa gtagagtaaa gtttcccacc cacaaatgtt tgaagtagat acttttcgcc
    11881 atttctaact cttgtaaaac ctgagcgaac gtatccacca taggtatttt cttttccatc
    11941 atttgtattc agaccaccaa ctgcccactg caaatctgca atgctcatac cggctaaaac
    12001 attgaccgct ttatttgttt tagaaagaga tagctcaaat ctatcagcat tttgggtgat
    12061 ggtgctttct gcagttgtaa tacgccctgt cagattatca accgtcgatt gacttgcctt
    12121 actagcaatc tgcccagctt gcactgtcag agttgattca gctgtgatta ctcgtccttc
    12181 aagagttgac ataccactcg ccactaatga aatcttgcta gtattctgtt caacggatgt
    12241 agtaagcgtt tcaacacttg cacctacact ttcagctgca ttcaaagcat tcacagcatc
    12301 gttaaatgct tgattggcct tttcagtggc agtattggca tccttttgtg cttgttccac
    12361 ggcttgcttt acttcttctg ccgtcatatc tgtcataagt ggataccact gagtgacgcc
    12421 atcacggatt tcatacaccc acatttcaaa cttgtcacca ttttctttgt accaaacgtc
    12481 accgagtttc ggattatttg gagttgtcgg gccatagaag ttggtgtttt taccatttgc
    12541 cgaactcaat gcgtaattgg catcgtcttt ggcttgctcc gccttgttgt ccacccggtt
    12601 gatgtccttc gccacatccg tgaattttgg cgtgatgttt ccgagtgtta ctgagatata
    12661 cgctttcaga aatggatcgt atttgtatga gaccattcta gctgtgatgt tcaatccatc
    12721 ttcggcatga ataactgaaa cagtatcacc caaattaatc gtttcaagct gtgcaaaatc
    12781 tttatattct tcagttcgct caagcggggc aaactccaca tcataggtgg cttttggcaa
    12841 atcgaccttg ttagctgagt attctaaccc agccaatctg cgaagttcat cataggcctc
    12901 ggcttgagta aactcatcct cttcatcacc aactttgacg ttgtcgtact tgatgacttt
    12961 gattttaggc tggacgtatt ggttgatgtt tggactgtcc acgtattttt cgggaagaaa
    13021 tagtccatca tacccttgtg gcataatgcg agtaacaatt ccagagtaat caacatcagc
    13081 tttatatcct gtcagattct tcttgtctcg gatttgtacg ccgttgttac tgccacgagt
    13141 cgttaacatt gaaatcgtga agttatcgcg tacaatttca ccaccataac tgctttgaaa
    13201 accattctct aggtcactat caagcaacac ctctacagga tttagtcgca ccaagcggac
    13261 attattgacc gcactgatgt ttgatgtacc ggtaaaacgg tgcatgaatt gagttgcacc
    13321 aagcagttgt gtcaaagctc ccgacccgtt cttgttgacg atataagtat cttcaataaa
    13381 gtttgtggct aggtcataga aaatatgatg agctactatg tgcaaaaaac caccaagtgc
    13441 atcatctcgt tcaacaatcc tgaaaagctg gtcggacagt tctggtactg gtgctttcac
    13501 aatcatttct ggcaacaatt cctcactgtg tggagcatga attggataat cgaattcaaa
    13561 ggaaaagagt ccgttcaatt cctcactcac cacggggttc agaatgttgc catcaaggac
    13621 agcgagaccg ttatgattga aatttgattc tgttttgttg taaagcgtaa tcataagtat
    13681 cgccacctcg gttctatttc tactttggtt atacctgtac caagagtcac agtattactg
    13741 ccgactcgaa actcaggaaa gtcacctgtc atgtattgat tggctgccac atccccgtaa
    13801 aagcattctt tcaaatccga atctagtgtc aggttgccat tcgtcagatt taacacgata
    13861 ggcttgccat ttatcgtgag tgtcctagtt ccattgccgt agaccttaat cttcggcaat
    13921 gagtaaatgt tgccgggatt attgaccgtt cctgacgtgg ttcgattgat agtcgctaca
    13981 ttggtcttat aacgaaaagg cgaacaggtt aactccattt caaactccca catggaagat
    14041 aaaatctgag ttagtccgct cgccttagca tacttgattg ggtaaaacac gtttggttca
    14101 tttgaaaatg cgatagtttt agcgtttagg atttgtggca aaattatctg ccagctagtc
    14161 caaacatcag atttccaaat cactacctta aaaccgaaag tcacatcttt ccagcctttc
    14221 agaatggtca aatctccttc acgtccatca atctcaatac tatcaatgac acgttccgtc
    14281 actggaataa ctggcacttc cgtaattcgc aaacctaaat cagtccgtga attaattgtt
    14341 ccgtctaaaa taaatgcgtt caattgacca ccgcccatct aaataactaa aaaaggacca
    14401 gacaacaatg tctaatccta aagtttataa tctatcatcc tattcggttg tttcttccag
    14461 ccctattttc ttggtaatcg ctaaatacgc gtatacaacc agcacgccgg aaattccata
    14521 tattgcatcg attagatctg gtgtattcag aaaagtcatc atcgtttcac atacaatatt
    14581 tgcaacaatc agaccagctc ccatcgcagt aagaaccctt aaattcgttc ccataagcaa
    14641 ccaaagatag cctatcacca aatagaaaag aaagcttaat gcgaagtttg aaatgtgaga
    14701 attaatctga ttgttgatga atacaaaacc atgatttatg ccgctatacc atcttactgc
    14761 cgttaaaaat aatccaatag caagcagtag aatatatcct atcagtgcag gtttctttgc
    14821 tttaaattta tgcttcaaca tatgattacc accatctctt cagtaaacac tcgttagtta
    14881 tagtatacag tcaaaatgtg aaaatccaac actaatatgc tacacgtcta cgattcaacg
    14941 tcgccaagct cttatcaatt tctggagcaa gtttgccgac caatgttcca tcattcaaga
    15001 cgactttaat gtccattgct ttaagtagcg ttggcaacat ttcactcgtg atgtttgtta
    15061 aggcatcaac cttagcacct aaatcaagca tcgagaatgt gctatcaacg gcagcgtgga
    15121 tatcggtatc aatatcaaag cttggcatat aaagttttgt tggaatagct ttctgcatcg
    15181 ttttagaaac accagtcatt tccttatcaa aacctacgcc aatcccttgt gccatgtact
    15241 tcccgatttc atcacgaaac agtgttgatg gtgaatgaat accaaagaag tccttgatgt
    15301 tgtctactac accgccaaag aatcctgaaa tcttgttgcg taaccaactt ccagcatcag
    15361 cgataccatt ccacaatcct gtaatcaagt tcttcccgat agttcccata gttgaaacat
    15421 actcaccgaa agcattcgca agccctgtga tgatttgcgg tactgctttg acaatggcag
    15481 aaatgattgc tggtaagttt tgaactaatg ccacaagcaa cttcacacca gcattgatga
    15541 ttttgtcgat gttgtttacc aaagttcctg ttattccgtt gataatttgc ggcatggcat
    15601 tcgcaatcgt tgtgataatt tgcggtaacg caccaatcaa tgccacaagt aactgaatac
    15661 ctgcgtcaat caactgaggg attgccccaa tcacggcatt gatgatattt tgaataatca
    15721 ctggaatagc tgcaacaatc gcattgatga tatccggcaa agctgtcaca agtgatgtca
    15781 aaagttgaat accagtctga atgatttgtg gaatagcact gataataaag ttcacgattc
    15841 ctgtaataat ctgtggcaga ctttcaatca gtattggaag tgctgtgata agtccttggg
    15901 caagtcccat gataagttgt aaagcagcat ccaacagtaa tggcaagttt tcaatcaagc
    15961 cgttcacaat cgttatgatt gcctgtactg taattggaat aagttctggc agcgcttcgc
    16021 ctagtccttg gataagtgtt gtcacaagtt gaaaggcagc gtcaatcagt aaaggtaagt
    16081 tctcaataag tgtttgaaca atcgttaaaa cggcttgaac caccactgga ataagtgtgg
    16141 gaagtaagcc aagtatcgtt tcaagcactt gtgagaataa tgaggttact gcttctaaga
    16201 gtgttggcaa taactgcccc aatgcttgaa taatggcatc caccactgcc ggtaaagccg
    16261 acacgatatt ttgaatgacc ggtagaatat tcttgatgac gttttgaaat tgttccacca
    16321 cattgccaat caacatacca atgtcagcgc tgctgttacc aagtccagcc atcaagtttg
    16381 agattgcaga cttcataccg tcaatcgaac cacttatcgt ctcactcgct tccttggcgg
    16441 ttgtaccagt gatgcccatt tcggtttgaa taacgtgaat cgcttcagtc acatcagcga
    16501 agttcgagat atcatatttg acaccagaaa tcttctcagc ctcttggagc aaacgttcca
    16561 tttcggactt cgtgccccca aaacctaatt tcaagttgtc cagcatcccg taattttgct
    16621 tggcgaaacc ttgataggcg ttctggatgg actcaatatc cgtccccatc ttgtttgcat
    16681 tatcgctcat atcagtaaca gcttgatccg caactcctgc ggctttcttg gtatcaccac
    16741 cgagagatga aatcaaggca gctgagaaac ctgtgacagt ttccatgtat tcattggctg
    16801 acattccggc agttttgaaa gcattgtcgg cattcttttg aacggtggat gacgcatcct
    16861 tgaaaagagt atccacccca ccgactaatt gttcatagtc cgcgtaagca gataccactt
    16921 gttttcctag ctccacggca gcagcgccag cagcaagtgc cactgtaccc attgctaccc
    16981 cgataccttt tagaacgcca ccgaactttt caaatttact tccagacttt tcagcctcgt
    17041 cacctgtatc cttgagttcg ttgccaagct catcagtttt cttagcagca tcaacttctt
    17101 cagtacccat ggcgtcaatt gccttctcgt tgttagcaac ttcacgctcc atattattga
    17161 gttgggcttt tgcattgttt aattgcactg tccaattctg agtacgctta tcgttttcac
    17221 caaaactgtc actagcgttc ttcaaagcat tttcaagggt ggtgattttt tctttctggg
    17281 catcaatcga tttgtttaag acttggttgc gagcagacag tgagctgatg cttttatcgt
    17341 ttttatcaaa ttcggatgat accagtttca tttctgaacc tagcaccttg aatgattggt
    17401 tgatttcacg caacgagttc ttaaactcct tttcaccttc aacgccaatc tttaaaccaa
    17461 aattatctgc caaataaatc accaccttcc cttaaattcc actcggtatc acatcatcaa
    17521 tgaataactc aactttcggc ttgctaatcc cagtaaattg cttatgacac tcccataaat
    17581 ccaaaaacag tccaagcgag cagtaccaga aatcatctga cgtcataccc atttgaactg
    17641 tcccatagta atacaggcga gtaaatgttt cttcatcact tactcgcctg ctttgttttt
    17701 tgaatctgtt tcactttcga tattgcgagc tgtgcccttg aacattgctt cagtgattgc
    17761 tgatttatat tcagccaact gcaacggact cgtcatcaac tccacatatt cagtcgtgag
    17821 ttcatctttc ttttctgtgt tcttcaagtt gtaaatcttg atggactgat tcgccaacag
    17881 cgtaatcaac cagatgattt catcaagcgc caattcaaag ttctcggatt tcaacaattt
    17941 ctcaccaagg ttatcaagcc caccgtagtg actggcgatt tctttcgtgg cttttgttgt
    18001 caaaatcagt tcatattcat ctccgccaag gctaatcttg gcactgcgtt cttcactcat
    18061 tgttgcctcc tagtctgctg tgaaatctgg ttcataaaca ctcgtgtacc atccattgat
    18121 agttgtttgt tcaactcccg tatcgttttc atcaacttct gctttccatg gatgcttacc
    18181 actagcatct aacttgttac gacgcatgac tgtaccctca attgttggtg ttgagaatgt
    18241 aatgtcgtca ccttttgttg ctaggcttgt gctgggcacg gcaaacttca ctcggtacaa
    18301 ccacaaatat ttgtatttgc cattcgattt acgagcacgg aaaccaatcg caactggtgt
    18361 gccaccatct tcggttgtcg aaattaagac accgttttga tccacggttg caccaacaag
    18421 cactgccgcc acagcacgtc caatatcatc aacaccgagc gttaactttc catttttaaa
    18481 ttctttcact acttctgatg caccatcatc ggcgtataaa gttgcttcag caagttccac
    18541 cgaaagctca gctgagattg ctttcgcaag catagcgggt gtgccgtatg tttcttcgcc
    18601 agtagttgcc gactctgtaa tcggtgcata atataattta tccaatccaa tcgttgccat
    18661 tttcagacct ccatttcata atgcttggcg acatcaatcg ccacatgata gtaatttgta
    18721 tctttttcaa atcctacaaa taatcgttca gtaatcgtaa actcgtgttt caacaagttc
    18781 tgaaccagtt gtttttgcgt gttcagatag ttgcctttca caaacaagga aatgcgaact
    18841 tcctgtacat caacggttgg aaagttatcc ccgtaaacat cgaacctgtc agcgagtggt
    18901 gtcagcacac aatacttgtc tggcgcttta tcactaaact cgcccgtttc aacaggaata
    18961 tgaagtgtgg caaaaagact gttcaattcc tctaaaatgt tcaaatgccg tttacctcac
    19021 tttccatttt tgatttcatt gtttcaatgc atgacttgcg actttgtgat tttgcaggtt
    19081 tcaagaatgg ctttgctggc tgaccgtgtt ttccatattc gagaatgttc gcaatcttag
    19141 catttgaact gccatcgatt cgaggctcat caaaaccaac cttgatgttc cagttgccgc
    19201 ttttatcttg acgagcttgt gtgataccaa gggctgattc aagttcacca gttgaacgac
    19261 ttggtacttt tgtatttgag ccaacaacgg cagttaagtt acttcggact ttgccttcca
    19321 ccacttcagc accactttct aaaaccttgg ggatgatttc atctgtcttg ttttctagtt
    19381 ttgaaacctt taacaagaaa tcttctggca tcttcatcaa tgctttagcc atccgcactc
    19441 acctccattt cagtagtcag catttcaagg tacatattgc gatttcggac attctccacc
    19501 gacacaatct gatacctttt ctgatctgaa atgacaacca tctctgtcgt aacctctaga
    19561 ttaggaatat aacgaatccg aaacaggcag gtcgctgaac taaaagtcgc catgtttacc
    19621 catttctcag tcgcattctt ttcctctttg tatgcatgga cagctgcaag aacttcatct
    19681 tgtttcactg aaaatccagc gctatccttt tggttcgtca ctttaacgat ttgtattcgt
    19741 tgattcatct ttccaaaact catacttgcc accgcctatc gagcctgagt aacagattga
    19801 ccgtttccca gacttgctta ctggcattga cgttatccgc aaagaagcca ccagttgaac
    19861 catcacggct ttcgtaaaag ttactggcta acacaataac cgcctgttca gtggttggcg
    19921 gcatttcatg ttctgaatag tagccagact caatatgttg atagctctca gcataagaaa
    19981 gagcggtcag aatcaatcgt tccaacagct catcatcttc gttatgctca agtatgaggt
    20041 ttgccttgac cttatttagt aagtcactca taggctactc cttaagatga ggcagatgct
    20101 ttcatcttca agatttgcac tgcttcaggt aacacaagtt taccgtccac acgttcttta
    20161 gctaagaaac caaccatgcc attacctgca aagagttcac gcaactggtc gaatgaacgc
    20221 acgccacggt cgccaatgtt atagtagctg aaatcaccaa aagcaatgac tggcgcacct
    20281 gccttaacgg ttggaacaaa ctccgaagta taaactggat aaccgagcag acggtcaggt
    20341 tcacctgcta ccaaagctgg ctgccacatg taggcaccat tgttgtcttt caacttacga
    20401 agcaagctga tggtttggtc gttcatgata aacttcgcgt ttttacgata tggacgtttc
    20461 aaagcataca caaggttcac cacatcatct gccgtaactg cactttgagt attagttgta
    20521 atggcaactt gtccgccacc tgttgctgca aaaataccga gtggtttacc aacaccgtca
    20581 ccattcaaga acgcatcttc ttccgcgttt gctaaggctt tggtgaattg gtcgatgata
    20641 tagttttcaa ggttaaaggc gttgtcataa agcaattcat cagtcacctt gattgccaca
    20701 tgaagtttgt gagcatcaag caagatttga tcgaacgttg catccccaaa gctcaactcg
    20761 ccaccttctt caatccatga agcggctggt ttagaacctg caatattaat cttgtgttca
    20821 ccacttgtcg taattgttgt cgcaagtgta cgcatgatgt tttcagaagt catgccgtca
    20881 atcaaacgtg agtcatattc ttctggcacc aaataaccgc ctgccgtatc cacaccttct
    20941 tgaagaacat ttgaaacatg tcggaagttt gagcgcaagg cttcaagtac accttcttta
    21001 tattcagctg aagcacgtcc tgttttctta ggttccattt tattttcagt agtcataggt
    21061 tgcgaagtta atggttggct cattggcttt tcaagttcac gatccatcgc ttccattgac
    21121 tgcatacgtt caatttccat cgtaaagttc ttcacttttg tttccatttc cgcataagtc
    21181 ttggcatctt cttcagacat caagccatct ttatcttgct ttgcttctac aaaagccttt
    21241 gctccgttcc aagcttgtgc acgtttttcc atcaattctt gaattttgtt catgggttag
    21301 ttcctccaat ttttcattaa attaagccga ctcattaacg aatcggctgg gatagtgtta
    21361 ttaatttctt ctcgttcctt tggttcaggt ggttcaatac gacacttctt agcaatcgct
    21421 gaagtcagtg aattagttac tttgacttcc gagtacatcg aattattaac cggaatatcc
    21481 gggtcaatct caccgatgat ttcatcggca aatcctaggt gaacagcttt atttgcgtcc
    21541 atccacgtct ccgcatccat catatgtgcc agttgcacac gcgataaatt cgttttacgt
    21601 tcataggcgt tgataatact tgctttcact tcatcaagca tggcaatggc tttctccata
    21661 tcaacttttt cgccaaacgc aaccgctgac ggattgtgaa tcatcaccat cgccacagga
    21721 ctcatgagta ccttgtcacc tgccattgca ataacacttg cggccgaagc ggcgatgccg
    21781 tcaatcttga ctgtgacttt acctttgtag tcagcaagca tgttgtaaat acttgcggca
    21841 gcgacacaat cgccaccacc cgaattaatc cagactgtaa cgttacctcc acctgaattt
    21901 aactcatctt tgaaaatctg cggtgtgact tcatcatcaa accagctctc ctcagcgatt
    21961 ggtccgttga ggtagagggt tctctctagg atttcctctt ggttgtcttg attcagaacc
    22021 gtttgattct tccacttcca aaacttcttc atcttgttcc tcgctttctt ctttgtttac
    22081 gccataagct gccccggctt gttcaattgg cagcatattg ccattgaagt aatatttcgc
    22141 accagcacca ccagggattg ggttcaagtc ctcaagttca cgcacatcat caggagatag
    22201 gaacccattt tgaataccgg ttgcatagcc ttgttgacgt gtggcatagt caccacgcaa
    22261 taagccatcc acattaaact ttatgaaata cttttctttt tcatcgcgac ttaacagtga
    22321 tttattaagt gcttgttccc aacgattgag ccatggtgat aatgagaact tcacaaaatc
    22381 caaactcaaa tgttcaacgt ttgagaaggt cgcgtggtct aagtcgccaa ccatgtgaag
    22441 gggcactcgg aatatccgtg cgatttcttc cagttgaaac ttacgtgttt caaggaactg
    22501 ggcttgttct ggtgacatgg aaataggcgt atacttcatg ccttcttcaa gtactgcaac
    22561 cttggccgca tttgaactgc caccaaatgt cgcattccaa ctttcacgaa gctttcctgg
    22621 gtctttcaaa gttccaggat gttctaggac accaccagga ttggcaccat tggcaaaaaa
    22681 tttagaacca tattcctctg ttgcaatcgc aagcccgata gcattcttag ccatggcaat
    22741 tggtgaataa ccaaccaagc catcaaagcc aaggccagga atgtgaagca cgtcagcttt
    22801 tgaaagtctg acttgcccga catcagcttg atataaataa tagatttcct tgttgtcatc
    22861 gcgattaacc gtcatcttat ctggcattaa aggatacaaa gctacaactt cacctttgcc
    22921 atttcggata atttgcgcat aggcgttacc ccaaagtagc aggtgagtca ttaaggtttc
    22981 acgaaataca aatgatgtca tttccgaatt tggttcatca tgcagcaaga aatacaacga
    23041 atgtttcatt gctttatctt tgccgccagc atctcggtat tggtaaaagt gtaatggcaa
    23101 tcctgccacg gcttctgata gcacgcgcac acatgagtac acagccgtca tctgcatagc
    23161 tgtttgttcg gtcacgcttt taccagctga tgagccgcca aacataaatc ggtatggatt
    23221 catgatggtt tgatttgttg gtttatcgcg tgatttgaat agtgatttta ttatgcccat
    23281 tgattgctcc tttcaaaatt tgagtatcaa aaaagcacct acgatttgta gatgctgtgt
    23341 tcacattaga tataaaaccg acttcctctt gacaaaatta tctcaattgc attatttaaa
    23401 aaggtattta cttcctctaa acatttcgat ttttcgtcag gtgttaagga ttttctttga
    23461 taaggaaaga atgaactact tggtgcaagt tttttaaacg tcatataatc ttcatcatcg
    23521 aagaaatctt gtaaataatt tagttctata aaacttttcc aatctttgaa tttagagaaa
    23581 tagtctttat tattaacgat aaatttatca gcgttagttg atgaaccata tcgtttatct
    23641 cttacatcag aataaaataa atcaggaaag tcatgatacg gccctttacc tttaacacca
    23701 ttgagagaag gttttgatac atatagtggc ataaaatttg caagagtatg acttaaatca
    23761 tagaatttta caaatttcac tttcatctct tcggtgatat catcggaata taaaatgact
    23821 ttcaaacgcc cataatgact atctggcatt gaaaagttct tttgattata gcatttcaat
    23881 aaccgtatcg ttgcaccagc aattgtgttg aaagaaataa tcgtttctcc cctataattc
    23941 ccaattcctt cagcatactt tgtcaaatct tcagttagaa tttttcccca ccattttcta
    24001 taaaacagtc tagaaattaa tgtgttgtcc gggtctgaag atattttatc tggtgctact
    24061 tgaatcaaat attcattcat acacatacac ctccaaagac aattatatca atggattgta
    24121 ataaaagctc atatttcaca gaaacaacaa tcccctatca tcataaacac tggcaccatt
    24181 atcatttcca caacgaattg cacgatccag agccataatt gtcgcgatgg caccatcaat
    24241 cttctcagtg gatttttctt tatcagcttt gatattacct gccgggtcag ttcggacgaa
    24301 aatgttgtcc atattccaac gaagaaccgg atgaccgcca tgggctaatt tctgttcaag
    24361 agtcaacttc atcaactctt tagtagccgg gctcatatcc ttgaaacctt gaccgaaagg
    24421 aaatacagta aatcccatac cttctaagtt ttgaaccatt tgaactgctc cccaacggtc
    24481 aaaggcaatc tcccggatat tgtatttctc acccagttcc tcaataaatt tttcaatgaa
    24541 accatagtgg actacgtttc cctcagtggt ttgtatgtac ccctgcttgg cccacaaatc
    24601 ataattcaca tggtcgcgtt taactcgtaa gtccaaggta tcttctggca cccagtaata
    24661 tggcagtatc acaaacttgt catcctcatc ttcaggtgga aacaccaaaa cgaaggatgt
    24721 aaggtcggtc gtgctggaaa ggtcgagacc tccgtaacaa actcgacctc tcaaactttc
    24781 ttcatctgca ttgaatgaac aagcgtccca tttatccatt ggcatccaac gcactgcttg
    24841 tttcacccat tggttcaggc gaagctgtct gaatgagttt tcttcagctg gattttgttt
    24901 agccgaatca caagcagcca ccaccttttc ttctgcaatc gttatcccaa gtgacggatt
    24961 ggctttcttc caaacttctg gatcagtcca atcatcatct tgcttgatac cgtaaattaa
    25021 cggatagaat gttttatcat gctttcgacc ttcaataatg tcgagagctt tttgatgctg
    25081 ctcataacaa attgaattgg tatcattccc tgccgttgta atcaaaaaga atagcggttg
    25141 cgttctagca tcccctgccc ctttcgtcat gacatcaaac agctttctgt taggttgtgc
    25201 gtgaagctca tcaaatacaa ccgcagaact gtttaatccg tgtttagagt atgcttcagc
    25261 tgataatact tgatagaaac tattcgttgg ctgataaata attcgttttt gtgaagtcag
    25321 gattttcact cgtttgctta acgctgggtt ctggcgaatc atatcaacag ctacgtcaaa
    25381 tacgattgaa gcctgcgctc gatcagcagc acagccatat acctcagcgc gttcttcccc
    25441 atcaccgcaa gtcaaaagca aagccacggc agccgctaac tcactcttac cattcttctt
    25501 aggtatttca acataagccg tattgaattg tctgtagcca tttgccttaa tcgttccgaa
    25561 taagtcacgg ataatctttt cttgccaagg catcagtttg aacggtttcc cgaaccactg
    25621 acctttcgtg tgtttcaaca actcaataaa ggccacggca taatctgcac gtgacttatc
    25681 ataatgggag tctttgtcca taaattcagt tggtttgtaa ttatcgattg gaactcactc
    25741 ccttctacgc aaaaagcagc tgttgtcagc tgccttaatc tctttattcc atcatatcgg
    25801 caatgaactg aatggcttca gggttgttct tgttatcccg cagcaattgc accagttctt
    25861 gccaatcgta gtatccttct tcaatgccat cttcattatc tggcatgtaa atttgataat
    25921 ccatcattta tcaccctcca agataaagcg gctgtactca tctttgtgtt cttcaatgaa
    25981 aatcaccaac tcgtaaaagt gccggtcatg tgcaattcgc tgcactgcaa tcgcatcaaa
    26041 catatttgtc agaccacttt ctgcaatctc aatgatttgt tgcttaatct ttgcgttcat
    26101 tttgaccgtc ctttctgcct gcctcatagg ccgcaattaa ggcatcttcc aaacaccaaa
    26161 ccgcaatgtc caagaagtct tcacggtcgc tgttgtgggc cttcaggtcg ccacgttcag
    26221 gcaactgaac gatgtaattc tttgcgatgt tgtaaatctt acgtttactc actttcatct
    26281 taaagcccct ttctgatgta gatggtatct tctgtttttg actcaaggtc aacccagacc
    26341 aaatcgtggt tcaatatcat gtccgtcacg tattgccaag catcattgaa ggtttcaaat
    26401 ccttctcttc taccgttttg aaagaccaat tcgaagtctg gtttgatgat gtgcaccaca
    26461 tcttcgccgt ataccgcgtt cagtgttgaa ccgttgtccc attggataag gattgaacca
    26521 atgtcgtcaa caccggttac tgtgccttgt gtgcccttcg ctggggcatg tgggtcgttc
    26581 atcttgatga gttctacacg actaccttca gggtaacgtt ttttcatacc ctcgatttgt
    26641 tcatgagttg gaaacataac tttgtcttcc gtattctttt ggtagtctat atatcactct
    26701 aaagggattt tatagcaagt gatacgttat ttattcagga ttttctaggt cattaaatgc
    26761 gatagtcttt ccatctcgaa tcactgaaac gtctgcatcg cttccagcct gctcgatata
    26821 acgcttaaca atcacatcac agaacttttc atccagttcg atcgtatggc aaatccggtc
    26881 ggtctgttcg caagcaatca aagttgatcc gctgccacca aaactatcca acacgatgca
    26941 gttgctgagt gacgaattgg taatcggata tgccaacagc ggaattggtt tcatcgttgg
    27001 gtggtcacca ttcttctttg gtttatcaaa ttcccagatg gtcgattcct tacgtccggt
    27061 gtaccattga tgtttgcctt tcttcttcca gccatagagc accggctcat gctgccattg
    27121 gtaaggcgaa cgaccaagca ctagactctg tttcttccag atgcacgtac ctgataaata
    27181 gaaccctgca tccacgaatg cttttctgaa gttcaagcct tccgtatccg catggaaaac
    27241 ataaatactg gcgtcgttgg ccatcacact ttccatacaa gaaaaggcat catacaagaa
    27301 gttgtaaaat gcctcgtcct tcagattatc atttttaatc gttcccgctt taccttgata
    27361 tgagacccca taaggtggat ctgtcaccac aagatttgct agtttgccgt ccatgagttt
    27421 ctcataagtt tcaggttttg ttgagtcacc acaaactaaa cggtgacgac caagcaacca
    27481 caagtcccct gttttactga aggctggctt ttcaagttcc tcatctacat caaagtcatc
    27541 atcttgggca tcatcattac cgttgagtaa tttatcaagt tcatcagttt caaaaccaag
    27601 caaatccaaa tcaaactctt caccttgcag gtctgataac tcaaccgcca acatttcatc
    27661 atcccaaccg gcgttcattg ctagtcggtt atcagcaagt atgtacgcac gcttttgtgc
    27721 ctcggttagg tgttccacaa acacgcacgg tacctgcttg ataccttctt ctttagccgc
    27781 aagaatacgt ccgtgacctg caatcaagtt ataatcctta tcaacaatca ccggatttat
    27841 gaacccaaac tcacgcatag aagcttgaat ctgtttcact tgatccttac tgtgggtacg
    27901 ggcatttctg gcataaggga ccagcttatc aatatcaact ttctcaaaac gttctgttgt
    27961 gttcattaga acaaccccca ttctgctagt ttctcaaacc cgccaagctc attcacataa
    28021 tcacgcgcaa cttccacgat ttcagaataa ggcacgccat caatctcagt gtcaccaatc
    28081 gcacagctta actcaacggg cataccagtt tcttgagctt tcaagaatgc gtagatgttc
    28141 actgacacat cagcttttga taagtctttc ccatggatac cgccacctgt tactccgtct
    28201 gccatatcgg aaccaagctt gcgattagtc gcacctgtat ccacatccgg gccgccagtc
    28261 caatcaccta gagggttgac tgtagcgttt ggatattttt gttttaactc atttgtcttt
    28321 gcgtggctct gacaaattat caagcgttca ccatcaagaa tgtactttcc atcaaatgga
    28381 tggtcctgat ataagtcacg cgcaattttt gagagctgta actgttcatc agtcaatggc
    28441 atcccacgaa agattccatt atcgccacaa cgaatttcag tcgcttgatt gtcagccaac
    28501 tctttatctt ggggcacaat caccacatct tttttaattt tgcctgcgat acgattaatc
    28561 gcttggctga tttcattcaa atttaaagca gttgaagttt caataatcac atgacagatg
    28621 ccatgaccaa ttaaaacctc aactgctatc tttggattat tttcttctgc gtatgccaag
    28681 tccacaatgg ctccggcaat acgatctgca actttatctg gatgttgcgg attaaccttc
    28741 tctatcataa attctgttcc tttcaaccat tcttagaacg taacaatcgt tccatcgcat
    28801 ccgtattcgg atctcctgtg tattccactg atgcattctc ttttacaatc tgttgaatct
    28861 cataccacgt cacatttgct tgcttttgga agttctgcga catcgccaca aatggggagg
    28921 ccatgactgc cttggtagtc gggtgttttc cgagcaaacc atattgactc agagcatttt
    28981 cacattgaac aaaacgcgca aagttctgag cgtaggactc aaccattcgt ggggctatca
    29041 acgtttcgca tccgcggtct ttcaaccatt ggaacgttga gttataaatt tcatcagcgc
    29101 ctagcacagt gccatccttc tgttttgcag aaaggtaagc acttggttca ggataatcat
    29161 tgccctcaag ctctacaccc tcaccaatat cttcaccaat taataattca acatccggta
    29221 agtcaaattc catccgcgat gctttctttc cagcagcaac tttatcaacg agtggctgtg
    29281 agcgttcccc tgaacgaggt cgtctgccac cgcgttgtgt tccatctttt gccaaaattt
    29341 atcacctctt tttcttgttt ggggctatcc cccgtttgaa ctgaactttt ttcgcgtaag
    29401 ggcctagcgc gctgtacagt ttagaagggt ttagggataa ttaccgcccc tccccccctt
    29461 gttatttttc acatgattgt tttatctttt gtgtttcgtc gagtcatgtg aattttcgag
    29521 tgacatgatt tgcagacact catcaaatta ttttcatcat gcgtaccacc atcacttaat
    29581 tccttgatgt gatgcacaat ctcaaccgaa gtaaagcgtc cttccttcag acacatctca
    29641 cataggggat gtgctgccac gtaccttgcc cgtattttct tccacgctct gccgtaacgt
    29701 ttgttgattg ccggatcacg ctcgtatttc tcgtaacgtt tcctcgcaag tgttttatgt
    29761 tcatcacaat aagtatcatg cgtacggttc ggacaacctg ggaacttaca ggggttagct
    29821 ggtttcattg gcaagtgggg tcacatcctt tcgggcaaaa gaaaagccct tgaaggattt
    29881 ttcccacaaa ggcttcgagt atttgttcga tacttctatt gctggtattg cttgagtatc
    29941 gtttgtcttt tttgacatct taatgatatc actaaaggct actgacattc actgacacat
    30001 ttcaaatgac tatgggttca tctggtagct tcaagttctc aagagctttg ctatgccaac
    30061 gaaagactgt ccgcacgtca gcgtgaagat cttcagcaat cttgtcccag ctctcttcca
    30121 acaggtatcg attgattaac acaatctttt cgtccacgtt tgtgagttca tcaatcacgc
    30181 cacgcatttg tcgtttgagg tctacaaact tatcaatctc tgcatcaatt tcagcttcca
    30241 attggtaaca cttctcaagg ttacgaacaa atggtgcctc agtattccgt gtgccacttg
    30301 aaatcttttc ttcgaatgtt gtggaagaaa ctgtcaccgc cagttgatag agctccgcct
    30361 tctctttaat ccggtcgtca attcgtttat tcaaacggta cgcttgtttc aagtattctt
    30421 ttggtgtcac ttgcttccct ccaatctagc tttcactgca tcaatcaaac atgattgtgt
    30481 tttatctttt cgtttcaaag ccaacatcac atcttcatca atcgtatctt tcgcaataat
    30541 gtgatgaatg accacagtat cactttgccc ttgtcgccac agccttgcat tggtttgttg
    30601 gtaaagttcc aaactccacg taagaccaaa ccatacaagt gttgaaccac cagcttgtaa
    30661 attcaatcca tgtcctgcac tagccggatg aatgacagcg atggggatgc taccttcgtt
    30721 ccaatcaata atgtcctgag acgttttgat ctgtcgaacg ttgaatcgtt ccttgatacg
    30781 ctccagatca tgctggaacc aataggcaat cagaatcggt ttgccatttg taccttcaat
    30841 caaatcttcc aaagcatcta acttctggtc atggatgtga tgactcacat taaactcatc
    30901 atagattgct ccattcgcca tttgtagcag tttattcgaa agcaccgctg tattggctgc
    30961 atcaatggtg gcttctttca attgtagcac caagtctgcc ttaatttcat cgtacttttg
    31021 cttttctttg agtcccatat caactggcac ttcattcatc acaagctctg gcatatccaa
    31081 gaaatcgact gacttcattg aaatcgtgat atctgaaatc ttgtcgtaga tttcttgttc
    31141 tgcatcttcc cttggtttcc aagaataaat ctgcatgcca ttttgcttgt caggtagaaa
    31201 gtatttatct cgataacgtg aaatataata gcccaatctc tcgcccatat ccagaacctt
    31261 gaactccgca aacaaatcca tcaaaccatt acttgaaggt gttccggtca atccaacaat
    31321 tcggtcaacc ttgtgtctga ctttcataaa gctagtgaac cgtttagctt ttaagttttt
    31381 gaaggacgac agttcgtcaa tcaccaccat atcaaaatca aacgtaaaac ccgatttgtt
    31441 aatcagccag tccaagttct cacgattgat cagatagatg ttggcattat tttgtaatgc
    31501 tgccattcgc tgacttggtg ttccaagaac cacggaatac gataaatctt tcaaatgatc
    31561 ccacttttga atttcagttg gccatgttga ttgcgtcacg cgcaacggtg caataattaa
    31621 gacacgttgt attgtaaagt catcatgcat caagtcttta atagctgtca gcgttgtcac
    31681 cgttttaccc aagcccatgt ccaatagaag ggcggcagtt ttatgatctt tgataaaatc
    31741 aatcgcatat ttctggtagt tatgtggtac gaacttcatc agacatcacc tcctattctt
    31801 tctagtaact caggtatctg atcaatctca tcaagcacga aacactgaaa ccctaaatct
    31861 gtcagttgtt tcatacgaaa aagctgtaaa gaccgtggct tcttccctgg cgccttcact
    31921 tcgacaaatc caagtttccc atttggcata agtaccaatc ggtcaggcac accagcaagc
    31981 cccggtgatg taaacttcgg gcaaatacca cctacccttt tgacggcttt cactagtgct
    32041 tgctcaacct gcttttctct catagccaca ccgtcaaata ctcgaaatac gcatcacgca
    32101 agatttccaa gtattccttt tctgcgcagt gaatcatcag atgtgcgact tgcttatcga
    32161 atgatttctt ccaaggatac gtgacatccg acatggcaaa ctcgtaaaac tcatcaaatc
    32221 gtaaatcacg tttaccgatt ccaatttgta accactgttt aaatttcatt tagatattct
    32281 ccaatctgca agtatggtgt aacccgtgaa tacgcaatac ataactttat ataggtggta
    32341 attttttatc ccttaagaaa agttctatat aagacctaca caggttacac tttcctttaa
    32401 aatcaactat tccaagaact cggatttgag ttgcaaacca acaatcttgt ttcctttatt
    32461 agttcgccgg cgcttaaatc cgcccgcctc cagagccgtg taaaaatcgg ctgaactacg
    32521 aatgtactca ccagttcgcg tacaaaacgc acgatactct gcatacaact caccagattt
    32581 ttcttcaaag tcaacagcag tctcgcagca ctcgtctaag aaatggccaa gccaatcatt
    32641 cgccgtctta tattcagaaa ttgcattagc gacaacttgt ggaaccgtta aatgaaagtc
    32701 tgcggcgatg acttttttcg caccgtcaag gatccattgc aaaatggctc cgccggcatt
    32761 ttcaaaaagg tagtccgcat aattcttgat gtccttattg ccgtcaatcg tagcaaggaa
    32821 tggaatcaca attaaacgac gccaggttcc tttatcaatc gctccaactt ttgggaggtg
    32881 gttggtatag agcaccaaag tatgcgttgg gacatacttg aatgggtctt tgtatttctt
    32941 ctcagccgca atttcatccg tcgagcagag ctgcttaatg ttagatgtgt tgagtcgcat
    33001 cccctcttca agctcggctg cgatgagtag gcgtttcccc ttcgcttccg caagttcagg
    33061 tttcacattg cgtcggattt gacttgtaag aatatcagct gagatgctgc cagagtaatt
    33121 accgagcaca cggctgatga cattccagaa cgtggacttc ccgttacgcc cttcaccata
    33181 cgagatgata agcgcttcaa cgtaaacttt accaatagct gcaagtccta caatcatctg
    33241 gacataatca atcaactctt ggtcacctac gaaaatggtg ttcagagcgt ccaaccaaat
    33301 ctgctgattg gcgtcactcg gggcacattc cgtttgcttc gtgatgtaat cttctgagtt
    33361 gtggtcttgt gatttgcctg ttcgcaaatc aaaggttgct gttggtgtgt tcagcaagaa
    33421 ctcgttcaca tctagtaacc gttgctcgat ttcaagcatg ggctttgcct cggttaatgc
    33481 tgacgagaga tacttgctgt cgcggcgttt gacagcaaac ttcttatact cttgtgcgtg
    33541 ttggtacaaa tcaaaactat gacgttgcgc tgtgttgaac atggtgactg cctttttcgg
    33601 accaacacta gcaagtacat cgaacgcgcc attactaacc atttccttgg tacgcttctc
    33661 aatttcggtt tcagattctt ccagttgacg ttctgtcaat gcgtggatca caccttgcgc
    33721 ttttggtttc gactcttccc aatagctgcc gttgtagaca aggtaaccag ttgacggtga
    33781 gtaatgaagc ttacattcat actcgcgagc taagaccgtt gcttgaccta agtccgagta
    33841 gtcggttggc tcaagtgata aatcagagtt gtactgctct ggaggaatgt agccttcttg
    33901 ttgtttcact ttcttcccaa agttgaccgc gctattccag atagctttaa gctcctcctc
    33961 cggcagtggc ggattacatt tttgggattg ttcgagatac ttgtgatacg tatcttccgt
    34021 tgcaccgtag cgtttgatga gctttcctgc gatgtgactc atgcttgaat tacggctgcc
    34081 ttcgctaatc tctgactgcg cattatccca atcagcaaac agatcatctt cgagaaaatc
    34141 agtgatcaac ttactacctt catagatttc aacttcagag tttggtgttc cgaaaagcaa
    34201 acgtgccgca tccatcgcat tgttgtcaaa gtatgggaac tcactttgaa tctgcttctt
    34261 gagttccaca tattcatctt tatcagttgt ggcaggaatc gggaaatata cgtgaaaacg
    34321 tggacgtgct gatttgccac ctttgtcttt catgttgtta cgtgaatagc tgaccgcaaa
    34381 cgtcacatca ggaaactgca tcgccacttc gaaaggggct acccaatcat ctggattgtc
    34441 tgagtggtca ttgtcgcaat caagtggaat cacatcagac tggatgaagt tgtcaccttt
    34501 gcgatagttg cctttgtact cagccatcac atgatccagc ttgaccgtgg caacaagtgt
    34561 ttctttatct gtcaccactt gcttggttgg gaacaatcca ttcttggaat tacccacctg
    34621 attggatgtg aataacgtca tcttaagcat ttgagacctc cttcatctca tcatcgaagt
    34681 acctgatatg tttcttccaa tactccgctt gttcaatttc aactgccatg ccttgggaga
    34741 tggtgtcacc caccacccag acttcctcac atttgcccat gagaacaagt gacatgtgca
    34801 ttgccacatc acgctccgtt gcgtcgtcca tgaattgtgg atacatcaga tgtgccgcaa
    34861 ctggcatgta gccacggtca agagcaatac ggcagtaacg cttagtgttt ttatggttga
    34921 cgacaacatt tcctgagaac ggcgagcaga catagactga tgttctgtaa tctttacgtc
    34981 taggcattat tatcacctcg ctcaatcact ggtaagatgt tcttcttcga tttcagcaag
    35041 tcgtacaaga acaaacgacc tttctgcgtc cagtaggtat gcatcacgct gcgctctgca
    35101 tctatcgcat gagtttttga ttgtgtgtag ccttgattgg catacttctg ataaagcaac
    35161 cacatcttgc cttgcttgta ctgaattccc agttcatgaa gcaacttgtt cattgcctgt
    35221 ggtgacatac cgtagtcctt cgagatttta gaaatcgaca tcacagaatt gttttgaaga
    35281 atcaaatcgt agtagctaac ttttggtttc atctccaaaa tcatttgttg ttgcatcgcc
    35341 accatttctt ttttctcttg tagctgcttc aacactgaaa tggcccatgc tggatcttcg
    35401 attgactttg tgatgaaatc ttctgtcgcg tacacaccgt gttggcggat agacggcaag
    35461 acctcatttg tcacccaacg cttgaatttc ttcgcgttcg gcatcttgct agaaatgatg
    35521 agtgagtaca atccactttc gttgataagg atagccattg tcccattaac gctgaacgat
    35581 tcgttcaccg tcttatcttc ctcatcaaca tgatctcgta atgctttttg tggatttgtg
    35641 tagccaagaa ttgatgctac atctttacca acaaagtgag ggacatcgcc aatcatcacc
    35701 gtgcgtacag agccaaactc cgcattttca aaaactttca attcgttcat cttgtgaacc
    35761 tcctgtaata gaattttcag gagaggcatt ttgccccttc taacttacag gcgaagaaaa
    35821 tggtcgaaag taaactctga ctcgaattta tttttgattt ttttgccttc gtctataagc
    35881 gaagaaagaa cccaattcga accttatttt tgataaaaaa atttgaaaaa aatatggagc
    35941 catttctgac tccacattga ctaatccttt tgatagaatt cacactcgta tccatcagcg
    36001 ttcagtagca agccttcagc ccacttaggc gtttgactca tcaactgaca gatgtcttca
    36061 accttcgatt ccattggcac ttcgataatc acttcgtcgt ggacgtgccc gacaatgcga
    36121 tacttacgaa gattctccat tgcgaaacag agaatgtctc tagctattgc ctgcacgatg
    36181 ttttcgacaa acttgggacc atagctttca agacgttccc atttcttggt agcaccgaca
    36241 ccttcatagg taacagcttc accgccgaat ttgttctcac caatctttgg ttttacataa
    36301 actaatttac gtcctgacaa gagttggatg aacagaaaac ccgacttgta ttcaaacttg
    36361 atgccatgcg tcgctgtgct ccgtcgttct ttcacacaag tctttactgc cttatcaaca
    36421 gcccaccaaa gctccgttat gttaatgttt gagtctcgcc aagaattaac aagcggctgt
    36481 aattcatcct cagttagtcc catttcaaga gcatccattg ctttcaatgc acccaccgat
    36541 cctccgtacc ctagagcaag ctcagcaatt ttcccttttt gacgtagatg cccattcacg
    36601 ccatgctttt caactggcac accgaacatc tgactggcag acgcacagta aatatcgcca
    36661 ccatttttga atacttcttg acgccacttc tcacccgaca gccaagcaat cacacgtgct
    36721 tcaatcgctg agaagtcaga tacgataaat ttcatccctt cacgtggtac aaacgctgtc
    36781 cggaggagtt ctgagagtac gttcgggaca ttgccgtaga gaatgttaag catttcaaaa
    36841 ttgctttgtt ttactaaact acgcgcttca gataaatctg acatatggtt ctgaggcaag
    36901 ttttgaagct ggatgccacg tcctgcaaag cgtccggtac ggttggctcc atagaattgg
    36961 aataaaccac gagaacgatt atcagaacac gcaaagtttt gcatggcttg atactttttc
    37021 acggatgact tggcgagttg ctgacgtaag accaggactt gctttaaatt ttcatcaata
    37081 ggttgttcga gtagttcggc cactgccttt ttaccgagtg actcggtttc aagtccgtgg
    37141 tctagtaacc atccccgcat ttgaagcacg ctgtttggat tttctagtcc agttaattct
    37201 tgcaattgtt gattcaatcc agcttttgat aattcatcca actgaacggc agcttccaca
    37261 aagtccttat caatctcaat tcctcggtcg ttgatttcct gatccagcca gtattctttc
    37321 caaacagaat caagcactgg aaaattggaa agttttcctt ggatagctat ttcaacttcc
    37381 acatcacgac tattgtatgt cttaaatgct gaccatttag ttggcgcatg gtgtggtaag
    37441 ttcctcgtgc gtccaccatt aactttggtc ggattgcatg gcacgcaaaa gtatcggatg
    37501 aggtcttttc cttctgtgag cttttgttta tccaaaccaa gaacagcacc aactcctaca
    37561 agtgacaacg gcaaacctaa tgtcgcaccc cacaccattg tgcattgcca ccctcgtgga
    37621 ttcaaaaagc catccaaccc cagaaagcgt gacaaacaca cacgttcaaa actcgcattg
    37681 aaggcgtgtt taatcactgt ttcatctgat agcgaagtta atatttcagc cgggatagtt
    37741 tcgtccatct tcagatcaac cacttcgacg ggaccaccat ctacactgta tgcaaatagc
    37801 agtatttcaa aatcggggga ctctgaatat ttgtagacac cacatttcga taaatcgaca
    37861 ctactatacg tttccaaatc caatgacaaa tgtttcattt tcttcctcca atcaaacagg
    37921 cgacagcact tctgccatgg ccgtttttgt tttaatgatt aagccaagaa atcatcatcc
    37981 gccaaggttt caaactcatc gcttgcagat acttgtgagc cgagtggttc gccgtcacgt
    38041 acaaactgga tattcccaag ccctgccgct accccgcgat ttccatttgc gttaaaggca
    38101 tagaaattaa tcgacacacg agcgtagcaa ccactataca cttgtgaacg atccaagatt
    38161 ggttgaacgt gctgatccac gatttgtggt gcagtggttg agttcgcatt aatgaagtaa
    38221 tggccttggt atgcttcatc gtcgcgttcg atatccccat cacgtaatgg cagcttgatt
    38281 tctgctttgt ttggtttctt cccaccgaat ttcgcaagtc cctcttcgat tgctgcatcg
    38341 attgcttttt caatggtatc aatcgttctc ttatctgatt ttggaatcaa gactgctgtt
    38401 gaatatttct ctgcgccccc gttgatggat gctggttccc aaccgtggaa gtatgagaga
    38461 cgtgtgtttg taccagtgat tactttgctt gttttagaca tattattgtt cctccgttaa
    38521 tggtttaaat tcttcgttta agttgtttac tgtgaccgct tgtcgtttat ctgaaccatc
    38581 gactagcgtc agcttgcctt ttggtttgat gatgtattcg cccaagattt cattgaattt
    38641 ctctttaccg agtgccttcg tcattgctgt gacaccaagt aactttttat catatgggtc
    38701 gtatccagcc ttgatgaccg ccgcagcgac atcattgtca ttagaatact tacgagtaga
    38761 gcgaccctcg actaatttgt gatgagtcca ttgtttgcca ccgagtgcaa gtttcaaagc
    38821 acagtccttg atatcgttac tccaagaaac caggtcatcc actttaccga tgatttcttc
    38881 gatttcatca tcactaagaa gctctggtgg tttgaactca tattgcgcca gtttcaagtt
    38941 atggtctgct cgtgcacgac agctattctt cgccggacag aatccacacc atttcccgca
    39001 ttggtactca ccctcacctt tgaaagctaa atctgcggca ggcgacaaca catcttttgc
    39061 ccactgaagt aattcttctt tcagcagtgt gtacgtactc acattctcac gccttggctg
    39121 gaaaatggtc atcttgactt cttgaatgtc ataaataaca tcgaaaagct ccaacgctcc
    39181 caaggcataa cacatcatct gtgggttctg atacgcatct accaaaattc ctagaccgta
    39241 tttcatgtca ataacagata gggttccatc cgcaatgacg agacagtcgc cggtcccaaa
    39301 gccgtctggg acgtaccttg agaagtcgag ccgctgttct actattacaa tagagtcaac
    39361 tgttgtctgt ttcgccttag cgacttcttc catgacgaag gtcgcataac tttcagcaca
    39421 ctgctccatc tcctcgtcgt agaattcaag cgtagatgtt gggtctgtga cgtcattacc
    39481 aatgaactta ttcagtttgt actcacataa ggtatgagca tcagtgcctt gttgggcata
    39541 cgggctcgac ttgttttcaa agtgctctga caagcgaaca cttggcggac agtgaatcca
    39601 ccgattactt gaagatgcgg ataggactgc gtgttgtgtt ggcatctaca atccctccgc
    39661 atctgcaacc aaagccgcgt agttttctgt cggcacatca ctcaagcgac tggcaccata
    39721 tttctcaatc agtgctttca cttcagttga cttaccttct tgagatttac gtgccaacaa
    39781 accacggact tcttcaagcg tcggttgttt gacttctgat tttgtttcca ttgtcggttc
    39841 tggtgtttca acttgtggtt caggttcatt ggttttcatc gccacagcca actcttgtaa
    39901 gctatcagct aacgcttgga cgtcctcaac gacctgaaga aatagttttg ttttactcac
    39961 gtcattacgc tcccttcttg ttaacttcac ggatttcgac tgattcgatt gtgtcccctg
    40021 gtgtgagcac gagaattttg tccatgttgc ccttaccgaa cagccaccgt gcaaaccagc
    40081 gaggcaaatg acgcacacca ccttgtagga cagtttcttt gttacccgtg tcatcagtca
    40141 cgtttactcg gattttgtgt tttaggttca tgatggtcct ccttcatctt tatttgttta
    40201 ggatctactc ccttacacaa tacaggcgaa ggaaaacggg gaaagtaaac tcccccgtaa
    40261 cttttttcta aaataattct ttgatcttgt ctttgatgat tccaaaggtg cgattgattg
    40321 actgtttttt cttgtcaagc ttgtccgcca cttcagtctg agacatgttt tcagtgacga
    40381 gcatgtgata gatttcttgg tggtacggta ataattgcgt cacgacttca cgcaaacgat
    40441 cgatacgagg atcttcttca ttatcttggt aagcatcgta aaccaatgat tggatggggt
    40501 tgtccccctc atcgcattca tcatcaaatt cattgagcga caaattgtag ttacgtttga
    40561 agtaatcgtt ggcttcacca tccacgtatt cgtccattgg ataatagccg tgctcattat
    40621 taaagtcttc acggaacttc tctttccaag ccttgatctt ttctttttct tcttctgtgc
    40681 gttcaggacg tgcgttttta taaaagttgt aaatcgcacg gtcgtcagcg cgatgcagct
    40741 cctgaatgtt aagctcagtg acgccgtctt cgcctgggcg aatctcaacc acttcttttt
    40801 ggatacgtcc attggcatca gtacgctcaa acacgtactt gtaagtgctg cggtcgtttt
    40861 ggtgtgtttt cataaatcta ctcattagat ccctctttct tgcctgaaag agcttttgag
    40921 caaggaaaac aaacagggac cgtgccaata cgtacaccgt ccctcatcac gaaaaaagag
    40981 cgcaataaag taaggcatac aatatctgaa aaattttctg gataaccagt tcttttcagt
    41041 atttgtaacc ttgccctatt gcaactcagg cattgttata ttttattagt aggactattc
    41101 gcttttaaga taaattcgtt cactttcttg aaacataatg tagtgtttat gaaccataaa
    41161 tgataaaatt aaatagttaa actactatgt tttaattata gaaaaaggcc tcccttgttt
    41221 tcgtcagtca tcggcggcaa acggcggtca acggcggacg taacaaagga ggttgtattt
    41281 tggaaataag tgaaattact caaacgcttt atccgtttat gggaagcgag tacgtgaagc
    41341 cacaggattt tttaattgct ttactggata gtgtttcaaa tgatgacgag gccaccattt
    41401 tcggtttttc tgatgataac ctacgaagat tttacaatgg tactcgacca ttaacacaag
    41461 agtatgcagt tcgggttgct ggaaaattca acatcgacaa atgcacgact tttatttatg
    41521 aacgtcttat agatgatgct ccaaccgtcc ttaagcaaca attgattgat aaaggtgtgg
    41581 taatccccaa agatacagac gtttctgaaa tagtggccca tcttttactt gatgaaatca
    41641 cctcaattgc ttctcaaaca aaagtaaaaa caaaaaaggc caagccgaag cttagcgaat
    41701 tagagctcgc aagtttaaga atcaacacta atggtgattt ctcttataat gatgaagtga
    41761 aaaaattctt tgacgatttg ccagttcccc ttgatattag agctgaggag atgacgtatg
    41821 tcatagctct tttttcagca tatgcagatg ccgatggata cgaagtatac gagcatacaa
    41881 acgacctgcc aaattcgcgg ctaaaggatt tcaagagaca gcgaagaaat tactataaag
    41941 ctgaaagcat acgtcgtggg gtccgtgata actttacaca agaggaagga actgagcact
    42001 ttgaagcttt aaaagaagat atgtacgacg gcatcgagga agtctatgaa gaagactacg
    42061 atgatggcgt tgagagactc aaagccgttc tacagcagtc gtctgttata acattaaacg
    42121 gatcaatctt atcgtttata ccgggattgc tgcaaaattc tgctaaaaaa ggcatttgcc
    42181 acatgctggt taatgacgga aagatcaaat gggtgactga agatgaatag tttgtttaat
    42241 actccatttg aaatttcggt tcgagtaatg ttactgctct ctgaatttaa agagcctgtc
    42301 agcgctgata gaattttaat cacagacttc atcaccacgt atggtcgcga ctttgaaatt
    42361 tccgattaca acttaaatgg tgataacagc tatagattca gtgaatttat tgcgcgacgt
    42421 gaattagtta aagatgcagt caaaaatctt gtactgcaac atgttatcca accatcgcaa
    42481 agcaaagaag gttttaagta tgctttatca gaagatggcc taaagttgat atcacaattc
    42541 gtatcggtat atgcagaaaa atacttacaa atagctgacg aatctgttaa atacattcaa
    42601 gataaaagtg acgttgagct tctgcgacaa ataaaccaac gcgcaaagga atttaaagga
    42661 gagcaatgat aatgcaaaat tattatattg aacgtgttac cataaaaggc aaagatcgtg
    42721 aagatgcaac tgtcaagttc accaatggac taaacataat ttctggccct tcaaataccg
    42781 gaaaaactag cattgttacg gcaatcgatt ttctctatgg cgcgaaaaaa gacgctgctc
    42841 ccttttcaac cgatgcaacc ggatacagta aagttgaact gattattcaa tcgactaatg
    42901 gcaaatattc attaactaga gaatttgata gcaactccat tttggtatcc aaaataacga
    42961 gttcaggtga atatgtagat tatgaaaaat ataatgcacg atctgggaaa aatccaatta
    43021 gccatttttg gttacaactt ttaggcatcg atgatgatta tcagattatc aagaatgagg
    43081 atttcaaacg tcaaaaatta acttggcgga cattagcgcc tagcacactt attccacaag
    43141 atagagtcaa taccagcgcc tctgttcttt ttcctgggga aacaacccaa cgcacagctt
    43201 ttttatcatc gttgttactt ctgctatacg gttcagattt tgcgaactcg gaagaaaacg
    43261 aaacaaaaga acataaaaaa attagaagga actcggtgat taactacatc aattcaagcc
    43321 tttctgaatt atatgaaaac tacgaagtgg ctaaatcaga attacctgag gctccctctg
    43381 atgattttca acaaattgtc gatggaatgt tcgctgaact tgaaacaatt gaaaagcaaa
    43441 tcaatgatgc gttagccagc agcaaaatct tgataaaaga gatttatcaa aaggaacagt
    43501 atcttcatga aactcaactt cttttgaata ggtacaaaca gttacattat caatatcaat
    43561 cggatattga acgactaaca ctaatcgtag atggtgagcg attattaaat gatcataatg
    43621 aacatgatac ttcacatagc tgtccattct gtaacggcga actaagtaaa cctgtccaaa
    43681 caacctacat cgatactgct aggtcagaat tacattcatt gctctctcaa ttatcagatt
    43741 tgacggaaga atctcatgat accgaagttg aactgcaaga gttaatggaa cagcttgatg
    43801 agcttaaaac aagaaagaaa tcgcttgatg cagcaatcaa cgatgaacta aaaccgaaag
    43861 ctgagacgtt aaaggatcga atcaataaaa tcgaaactta cagggatatt aaacaacagc
    43921 taatctcgat ggaaaaaatg caaaataagc ttaaaagtga tcttgaaaaa atcgagaatg
    43981 agccagaaga cgaacgtatc ctttataaac ctaaagagaa atttgatgca gaattctaca
    44041 aagaaatgtc tgagttactg gactccatgt tacgtaatat gcattataaa cctaatgaat
    44101 ttcgtaacgc atttttcgcc aaggatattt tcgacatcaa aatcaatgga aatctaaaat
    44161 ggaccgaaaa cggacatgga tatacagcgt ttatcaattc agtagtggta atggcatatc
    44221 gacatctctt gtataatcac gctaagtaca caccttctgt gttcattatc gattcccctc
    44281 ttcttggatt agatgaacgc aaaaaacaag atggcgatga caatatgaaa gaaggacttt
    44341 tcaaatactt tatggatcat caaaacgaag gacaacttat catcgttgag aatacaacga
    44401 acttgccagc actaaactat gatgataaac aagtaaatca gattgtcttt actgaagata
    44461 aaaacaatgg acgctatgga tatctatacg atatttataa ctagaacagg aatgattaca
    44521 tgaagtttag ctacaatccg ctttggaaac tcctaatcga cagaggactg aataaagcac
    44581 aactcagaaa aatgactgat atcagttcag caacactctc aaaactaaat aaaggcgaaa
    44641 atgtaacaac agatatctta cttcgcatct gtgaagcact tgattgtgat ttatcggata
    44701 ttgttgaaat cgttcgcgat taaatatcaa aaatgcactg tattcgattc atgagtacag
    44761 tgcattttca tatttatttt tgtaatccag cgattacttc attgattttc gattcaatat
    44821 cattcaatgg aaccatatac cattcagtag cttttttgaa gtttccatcc tgaaagacaa
    44881 cttgtttgtc ctggaataca tgatggagag tgcgctcaag tagctgagcg ttgacatttt
    44941 gtacttcagc agaaagcatg accttcactg gtgcatttaa gtaggtcacg tcgttttccg
    45001 cattggcaat tctactttca acatcatttc gagtgaaacc aatcttatac aggttattcc
    45061 acttgctaat atccggattc gtgcttaatg acttcagcac gtagatataa cctgtcgtaa
    45121 actcttctcc aaagctttca gccagtgttt cctcatctac cggctctgtc accatccttc
    45181 catgacgttc agtcgagtat agagacgaag ctaaagattg taacaacaga ttcttattct
    45241 ctgtcccatt ctcataaaca acatgcattc tggcatttgt tttcccatta tcagcgataa
    45301 aggtatcctt cgaaatggaa acgatataca tcattacgcc gttttggatg tagaatctat
    45361 tttcatgaat atcatactgc tcaaacggga taatttttcg tctgccagat gcgatatctg
    45421 catgcacttg atcgaacaag ctcttatact tttcaaaatg acttgcatgt tttctgcggc
    45481 caattttatc tgcagcgttc acagttctac gatagcggga aacatccaac aagtctgaaa
    45541 aatcatcaat catatcatct gagcctaaga tatcatctaa attcaactcg tcatcgattt
    45601 caactgaatc agcgagaaac tctccatccg gttcgaattc tttctccaat aaaccaagag
    45661 tgtcatactt cttaaccttc tcagaccttt cgggttttcc tcgaaatccg ctaagacgag
    45721 cccacaatgc gcgttcacta gaccattcag gagttttttc tggctctcgt ccgttttcat
    45781 tcacaaagtc tataatctcc tgaaatttag caacttctgg gtcttgtttt acaactttct
    45841 ttttttcaac ttgcttggtt aaatcatcaa aagcgtcatc ttcaaagatg ctatctaaac
    45901 tattcgccat cttctttcgc tgcctctctt tcacgtttta tctcagcaag tcgtagcaaa
    45961 gcaaaggcca agtatttttc accatcatct ttactatttc gatcaggatt tcgaccattt
    46021 tctgcacgga acttcttagc agcttggtag gccgcaacca attgttcgtc agtaaaatca
    46081 tacttatttg atgacaaata atcctgaata aaccgaagag tcggtgcatc aatatcacga
    46141 ctaatcactt catacgcacg ttggaacgga ttcacttgat cgattaaatc aatattcaat
    46201 tcatcaatgt taatgaaacg atcggccatc ttaataattt cgcggctacc atcttctgaa
    46261 gttgttgtcc gtccagccgc aatatttata tcggcaactg tatgttgacg aacggtttct
    46321 aactcttcgt tcgtcaaatc aggataacga tcaatgatga cacgtggaat caacgtctta
    46381 ttaatcatct tcgcatcagc acctgaaaca atcgcatttt gaatatcttg gctctgtaaa
    46441 atgctagatt tcaaatcttc catatcattt tcgatgattt tctttgtacg ttccgtcgta
    46501 tcagcaggtt tcagtcctct gattgaaaga tcacctggtt tgtctggctt accatctttt
    46561 ggtttaaact tgtaaactgg cgccaacact tgttccataa gtagagatgc agaaattgcc
    46621 ttcataatac tgttaaccgc gaataatact tcgtcatcct gtgcatctgg ctgcgcaata
    46681 agatttgtaa actgtgcatg agtcttattg gggctgtcac gagttacgcg accaatgatt
    46741 tgaatcactt cagttaatga gttacgataa ccgattgtca gtgcatattc cgcaaatggc
    46801 caatcgaagc cttcttttgc cattccaagt gcaatgatga tatcaagctt atcaagatct
    46861 ttaatgtcat ccgtaatact tgctagatat gctgagactt tttcacgtcc ttcttcattc
    46921 acaaggtcgg ccactaatag ttttttacca tctggacgtt cgatttcata aataccatcc
    46981 tcgttctgtg tgatattacc accgtccgtc aataaatcaa gtatttcatc aacttccgcg
    47041 tatttatctt tagtagactc accagaatta acattaggaa tatggataat tgattttttg
    47101 ttgacatcaa ttactttatc caaagcatct gtatattttc cacgatagaa actatacccc
    47161 attgcaaagg atttcagata tttatatcct tccagttgct cataataagt gtaagtgacc
    47221 ttatcaaatt tatcttcgtc ggctggttcc agaatctgaa cagagtcgcc acggaaatat
    47281 gaaccagtca ttgctaagat atgcgcatta gagtttgaca tgatgctacg taaggcatta
    47341 cctaacacag atttatcatc acggctcaca tgatggaact catcaatcgc taatagcgta
    47401 ttgtcaaata atgaatcgtc caactcttca aaagcaaatc gcaacgtagc atgtgtggca
    47461 atcaggatat tatcacttgg gtcagtcgag ttcatgaatt taacaaagtg ttgaaccttt
    47521 gatttttcac cgcccccaat agttaagttg tcacttggct ttacttccca atcccaataa
    47581 aaaccaaact tcgataattc agtatcttta aatgatttac caattgagcg ttcaggaact
    47641 gcaataacca cttttttaat tccttgatta tggagtttat ccaagccaac aaacatcaat
    47701 gcacgtgatt ttcctgaagc tggtggtgat tttaccaaca aatactgtga cgcacgcttt
    47761 tcataaacac gttcttgcat ttcacgcata ccgaggtcat ttgttgtttt tgattgattt
    47821 ttatcaaaat taatttcaaa taaattacta gtcattgatt gtcatctcct gatagagttt
    47881 tagtaatgtt ccaagtcgat cttcgtcaga ttcaaacggg cgttgctgat aagctctatc
    47941 aacaatccca tccagttcct ggtgtttctt acgtaatgat tctggcatac tgtctttatt
    48001 atacaaatcg gctaacgtgc cgccttcgta ttctcgtaaa tcaagaattt ctgtcatcac
    48061 gcgagtcatt tcatttttac gctgggcgga taaatcctga atcggaaaag tattatagac
    48121 taacccggct gaataacggt accgagtttc taatttccca ccgacagagc gcagccacac
    48181 catatgcatt cgggattcta acagaccaag cagccacatt ggagcgttat agattgccat
    48241 agccgaatct gaaattatag tatcgctccc aactataccc ataggaacat aatctctttt
    48301 ttctgatgac acacgaggaa ttagaatact attctcactg gtttcatcat aacgaacttc
    48361 accaaatgac catggcttct cagctagtgc tcgagtttct tttctcttgc ttttttccct
    48421 ataacttttt acatttttta ttgtttcttt aataagagaa ttttgtttgt atctagttgc
    48481 atcttcctcg gtcaaccata aagtatatct taagtctcca tttattaatc ctgttgatcc
    48541 taaatatttc ttaaataaat gtttaatatc tggatatttt tgaatcaaat tccagtattc
    48601 tgccactgaa aaaactagat taccaccatc atttgccata ctgccaaatt tcaaagaggg
    48661 taaatttgaa atactatttc ttgattcgtt gacaaataca ttttcaccat tagctaaata
    48721 tgcattaata tttgaaacac tcattactga cccattctct aaatataact taggatgaat
    48781 attcacatta tttgtcatac caataatgac aacagtcaca ccggcattat tctttgcatt
    48841 gttgttccac ttaaagctct gatatgcaaa tgaaattctt acctcatctt ttaaaaggta
    48901 cggccaaagg atactaactt gttcaccttg acaaattgaa ttagtagtaa caaatgcgaa
    48961 cgagccatga gatgttgcaa tatattgttt agctttccaa aaccacccaa aaatataatc
    49021 aagcaattta tattttcgaa tatcgccaac aagtttttcc atatcttcac gttgttcagc
    49081 attttgaaga cttttgccca gatacggtgg atttcccatt atatatattt catgattttc
    49141 tttgagtcgc aacacctcat tccaatcaat tcgaagagaa ttggcacaca ctatattccc
    49201 agcatctttt aacggtaaca actcagcaat ggtacctggt attgcttctt caagctcttt
    49261 attcatttga tgttcagcaa tccataatga aagtcttgct acttcatgcg caaaatcatc
    49321 caattcaatc ccattaaaat taccaagatg aatcactgat agtggcatgg aatcattatt
    49381 ttgtaagtcg cccaacaatt gcaaaatctt aatttcaagt ctacggattt ccttataagc
    49441 aatgatcaga aaattaccag aaccactggc gggatccaaa aacttaatat tagaaattcg
    49501 cgtcaacaaa gcacttagat cattgatgat tgttctctta ttatcgtttc ttgttttttc
    49561 agtaatgtcc ttcagctcgt tttcttcact gcgaagcttt aaagtttcaa atgactctgt
    49621 caaatcatct aagaataacg gcttgatgac tttcatgata ttcggcacgc ttgtataatg
    49681 cataccggca acgtgacggt cttctgctga agcaactgat tgaatcattg cgccaaggat
    49741 atcaggattg atttcattcc aattcaataa ttcccctgct tcaataagca gctctcttga
    49801 aacttttgaa aaggtcaaat ccacatgtgg ctcaccaaac agttttccat taacatatgg
    49861 aaattcaagt agccactcat ccttgccatt gcgagctaac tcattaatat ccaagattgc
    49921 aaagagttct ttgattactt cgttaaaatt cgaaccatct tcagccgttc ttgtttttag
    49981 aacatttgta aaggaaccct tattcataat tcctgtatct tctgcaaaca acaagaacag
    50041 cactcgaatc aagaacaagt taaatgcatg ctcgttggca tcaggattat ctttagccac
    50101 cacatcatac aattttgcaa aacgttcagc agcttttcgg tcagctggat tttcagcttg
    50161 ataatcagcc ttctcaatgc cattccacgc taagaaaaag tcagcattag ccggtagctc
    50221 ttcaattgga atattcaaag tttcatgagt tttcgtatca attgcagcaa tatctaaata
    50281 atcgtttacc aagatatatc gtggtttaga tttttgctct ttgattgatt gagcaatcgc
    50341 atcaatcgca atgacaacgt tgccttcaat ttcttcataa tgaagtttgt tcttaatcac
    50401 aaatggttca ccattatcga attttgattt tgcacgtgta atcgacgttt tggggatatc
    50461 gtacaaggac aaaaattgag tgatgaaatc atcgccccta tttgaaagta ttttttttac
    50521 accatcttca atttcagtga tgtttaaaac tttatttttt gccatttatt taacatcctc
    50581 cgcatctata acatctgaac taattatttc actaacatca acatttaagg cagcagcaat
    50641 tttcaataat gactccattg caaccaactc atttttaccg agttttgcta ttacattggg
    50701 actgacgcca gatatttcac gaagttgagt tttgttcata ttcaaatcaa tcaataactt
    50761 ccacaattta ttataagaaa ttgacatact acacctctat tctactggta tcttcaatta
    50821 tatcatctat gagctatcat tattatacga aacatgaaat attttcgcga acgcaaacta
    50881 tttttctaaa ataaaagcct tcagcataga tttactaaag gcgagataac aaatattaat
    50941 tccactttat tttaaccgga atccccaatt gacgagctag tttatcaatt ttgattctga
    51001 cattcttttt gttgaaattt gcataaaccc atactccatc agctaccctg gctgaatttg
    51061 gtcttccgtt atacatggat ggtttacgtc ctaaaattat agcgaacgac ggcgtctcat
    51121 cgacaattaa ttcttgcagt tgattgttgt agtttccatc cgcaaatagc tgagaaacaa
    51181 aatcaaccaa taaacctcta gcagatttcg aagatatctt tttcccatca tactcaattt
    51241 catattgtcc agatgaacta aattcataaa tttgggtttc actgtcttcg gaagtaccaa
    51301 acaaatcgat atttgcaatt tcatctataa tttcaaacac tgtgtcatat agctgatccg
    51361 attttgcttt ttgaagttta tcaatatacg aagtattacc agcagttgaa ttcatcattt
    51421 catagtcaga tttttcttgg aaatcttgga taagtcgttt ttcaagataa tctgtatctg
    51481 cttttgacat tttcccatct gctcttgtaa agaataaaac tgattcaacc caagctttat
    51541 attccttgag aaagtgttga gataaacgaa gtgaaattga ttgccctgct gcctgaccca
    51601 cgtaacgctt attaccacca atcaaaacat aaacagctgg ataatttgaa aaatcctgtt
    51661 ccttcaaata cggcaaatca ctacgactca ttgaaaccaa gattgctggc tcgcgttcaa
    51721 aataagaaat attatctttc tgtgtatctg aaaatataaa aatttgcttc ataagtttgt
    51781 ctcccttgaa aactcgataa tatattatat cacgtaaccg ttatcaaaat catctaacct
    51841 atcaagtcta cggtgtaact cctatcaact caaagctcca tagcatttcc atcaactcta
    51901 cgctcaccca aaaatcgtca tttcaacacg aaaaaagagc aatccggaaa ccatcccgaa
    51961 tcgctctaat ccttgtcata tcaacgttcc acacgtgtaa gcagtcctac acactcgatg
    52021 tgatgggttt gcgggaaaag atctacaggc tgaactttct tcacggcata acctgcttct
    52081 gcaaataact tagcatctct tgcaaaagtt gccggattac atgagatata gacgattcgt
    52141 tctggtgaca tttcgattgc tgattcaata aaatgatgat ctaatccttt tcgtggaggg
    52201 tcaactacga tcacagatgg tttgatccct ttttcttgcc attttttcat aactttttct
    52261 gcttttccga cttcataatg aacattttcg attttattat ttaaagcatt gaactgtgca
    52321 tcttcaattg cttctggaat aacttccatc ccatacacct tatccactct gtctgctaaa
    52381 gacaagccaa tcgtacctat tcctgaataa gcgtcgatca caatgtcttt tttatctaac
    52441 tcggcaaact cgatggctgt ttggtacaga tactctgctt gttgagtatt tacttgataa
    52501 aatgactttg ctgagattcg ataggttttt cctaatagct gatcttcgat atagtctctg
    52561 ccatataaaa ctttttcttt gtctcctaaa atcacatttg ttttttcttc attgatattt
    52621 tggatgaccg aaaccacttc tggcaattct tcatgaatag cttcagcaat ttccttggat
    52681 ttaaagaatt tttctttacg agtaactaaa acgaccatca tttcatggga ataatgacca
    52741 cgacggataa tgatgtgacg aagctgtcct tcattttttt cttcattata tcctcgtact
    52801 tggaaccgtt gcaagatatc tctgactact ttgattgctt gatcgatgac aggatcttgg
    52861 atataaaaat cttcgatagg gactagatca tggctattct ttttgtaaaa accggtggct
    52921 aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc gaatggttct
    52981 gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt cgccattaca
    53041 tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg ggctaaaggt
    53101 gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg tgattgtgta
    53161 ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac tagtatttct
    53221 acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat ttttgctacc
    53281 cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt tttctttact
    53341 atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta ctgaactcta
    53401 ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt tcagcaccgg
    53461 cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca ccgtcgatat
    53521 cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc tctaattctt
    53581 cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca aagaactcaa
    53641 tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca ccatctaagt
    53701 tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg cttgttttgg
    53761 tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt gctgcaatat
    53821 gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc ccatcatcca
    53881 atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc cctaggaaaa
    53941 acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt ttgattggct
    54001 ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca aaaatactct
    54061 ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca gcgatattga
    54121 tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt ttttttatca
    54181 cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca tagtcgttcg
    54241 ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg attccattaa
    54301 ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa cctagttctg
    54361 cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca taggcgctgg
    54421 cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt ttcaataatt
    54481 cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc ctctatcgtt
    54541 tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt cctttagttg
    54601 ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt ttgaaatcat
    54661 cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt ttgttcggat
    54721 cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg ccatttgtga
    54781 tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg gcgatcaaac
    54841 gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc agtttctcac
    54901 tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg gcatctgcac
    54961 cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc aataccattg
    55021 cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa gctggcatct
    55081 caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat ttcgggatat
    55141 caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc aaaactgttt
    55201 tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct gaaagaagaa
    55261 cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat aaagagttca
    55321 agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga agagaaatat
    55381 tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct gtaaattgga
    55441 taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc atatcagctt
    55501 cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa gaataaccgc
    55561 caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg atccggattt
    55621 ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt ggttgatcaa
    55681 attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc cggtcaaaat
    55741 gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg aattccagcg
    55801 cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg tttgtattgc
    55861 tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag ttggttttta
    55921 actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt tccctcctaa
    55981 aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg ccgcttgata
    56041 catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta atccttctga
    56101 taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga tcgttaagat
    56161 gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga atgcaactgt
    56221 tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa aatctcgttt
    56281 gatcaatgta cggacttgtc ccgctttttt gaagtaagcg tcataataac cagcacttaa
    56341 ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt ctgaacgtga
    56401 gttgacatat acatcatcta atgatttcac attttctgag cgataaccgt agcggatacc
    56461 atcgaatcgt tgcaagttgg aacttgcttc tgatgaggca atgatataat aaacagccac
    56521 accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg aacggaatgt
    56581 ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc ctaagtattc
    56641 tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg caaaatcagg
    56701 aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag ctgtcaatgc
    56761 taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag atgaagcaaa
    56821 ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta cgatgccgtt
    56881 aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa ctggaatttg
    56941 tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt ttgttgggtc
    57001 ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca tagcaaattc
    57061 atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat ccatgaccgt
    57121 tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg tcaagatatc
    57181 ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt tgctttcatc
    57241 aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat
    57301 gaatgcttcg acctcaggct ctgtttcttt gattcgttca aatgttgctt ttgtcaaatc
    57361 ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag aacgattgtg
    57421 taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac tttgataaag
    57481 ccgtcttctg tttccggcac atttttcaat aattcatcgc ggttccatcc agcttgcgcc
    57541 cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg aacaccttct
    57601 gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg gtcggtaaaa
    57661 cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt
    57721 tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt tgctctctat
    57781 tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa aaaactcatt
    57841 tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc ttctgtccaa
    57901 acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc tcctgctacc
    57961 acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt ttcgatcgtc
    58021 tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac tgttttttct
    58081 ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg
    58141 acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact gaaataagtg
    58201 actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga ctcatagtca
    58261 cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc tgctttcgct
    58321 ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc tttgctgttg
    58381 tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttgtccaa cgtcaataac
    58441 tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt atcatacatc
    58501 tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga aacaaagtgg
    58561 ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa ggctacttct
    58621 tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa ttggtattct
    58681 tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat gatgtctccc
    58741 gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat ataatcaccg
    58801 ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt catgatagct
    58861 gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat tagcgtttca
    58921 acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgac agtaaaacca
    58981 agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat ttcatagggc
    59041 aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc aatcgaatga
    59101 catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc tagcgcatca
    59161 tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa agtgtccaga
    59221 tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc ggcgttccga
    59281 ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg gacaaatgac
    59341 cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt taatttgatt
    59401 gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt tccatccccg
    59461 cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga aagtccatct
    59521 atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac acgttgatca
    59581 aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga
    59641 gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg agaatcagac
    59701 gtgatcaaat caggatattg ttcctcaatt gctactaatt ctgcgtattc tttatcataa
    59761 acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca ttggttcagt
    59821 cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc
    59881 atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa gacgttttac
    59941 tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc ttactcggac
    60001 gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc agctgttatt
    60061 ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt tatagctata
    60121 tgctgttttt ttagcgaata caggtgtttt tttaggcgta ggacgcatgc cttcgatttc
    60181 cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt gcgtccgtcc
    60241 atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc gcgtgatccc
    60301 cacatatgcg agacggcgtt cttcttccag ttcgctttct tccatcaatg ctcgagataa
    60361 aggaaaaacc ccctcttcta gaccaatcag gaatacaact gggaattcta atcctttggc
    60421 tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt caatatcgga
    60481 aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat cagcgtcttc
    60541 ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa
    60601 gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt ctagatatcc
    60661 gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga tcatctgagt
    60721 gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc caccgatatt
    60781 tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag aagcaaaact
    60841 gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc
    60901 aaaactgatt gaatcttgag gatttgccag cacattcaaa tacgctaaaa tatctttgat
    60961 ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat ttgctttcaa
    61021 cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc
    61081 gtaattccga tgattgcttc gaatctcttc ttgcatccga tctacgataa aacgggtttc
    61141 gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt tttctgtcca
    61201 caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg cgctaaggat
    61261 gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg gataatcttt
    61321 ttcaaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac tttgatctgc
    61381 atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta gcgtatactg
    61441 tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt tttgataata
    61501 tgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa
    61561 gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt ttgccgcaat
    61621 ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca ggctgttttt
    61681 tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat caatgttcag
    61741 ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt
    61801 ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca tcgagtgaaa
    61861 agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac gttctttcat
    61921 ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat tgacttcttt
    61981 ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac ttcctgcacc
    62041 tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac gggggttcat
    62101 cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta tcaatcttgt
    62161 taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat ttgtttcttt
    62221 ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt
    62281 ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat attttattct
    62341 aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc gattatctat
    62401 cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac agccgattta
    62461 cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa
    62521 aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc ttcgcgggga
    62581 attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg tactttcagt
    62641 tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg catccattgg
    62701 acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt taatttctag
    62761 cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat tactctttgt
    62821 tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat ttgcttcagc
    62881 aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt tatatctagg
    62941 tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag gagtacttct
    63001 aggcatctta ggttttattt atcagaaggt cttgctggct ttgccaaaat tttacaaccg
    63061 attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt tgccaattgg
    63121 ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt ttggacatgc
    63181 ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt tttccatgat
    63241 ttcttacggt ggtggtcttc caggggggat tttccttccg atattgacat tgggggcttt
    63301 aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa tctacatcaa
    63361 ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag caccgctgac
    63421 agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc ctttaggatt
    63481 ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc
    63541 attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg caaggacaac
    63601 ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac gagacttcaa
    63661 ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa tcttgactca
    63721 cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg aagcaaactt
    63781 gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga agcaatcatt
    63841 ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact tgattctata
    63901 tgattctata aaaaaagaag agactgggaa aaaccagcct cttctttttt atttctatgc
    63961 ttctgctgct gcaggagctt ttctcaataa accataaatc acaccagata cgactgctcc
    64021 gatagcaata aacaccaaat acatgaccgg gtgactcagt aagaatacaa cgaaaatccc
    64081 gccatgagga gccagtaatt tgatcccgaa agctccgact aatccgccag ttaaagcaga
    64141 accaataata aaacttggaa tggcacgaat cgggtcagct gctgcaaatg gaatcgatcc
    64201 ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt gatctgtttt
    64261 gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag gtaccattcc
    64321 tccagccata acagaagcca tcacgacact tccgccagtt gcaacagtcg tagctaaagt
    64381 tgctgtacca aacacataag cggctttgtt gattggaccg cctaaatctg ccgccatcat
    64441 tccagctaat aatgcaccaa gtaaagcagc attcgaacca ctcatacttg tcaagaaacc
    64501 gttcaatgca tcattgattg ctttcatagg gacattgacc agcaacatca agaaaccagt
    64561 aatcagtaag ccaaacactg gataaaagag gatcgtttta attccatcca atgattttgg
    64621 taagttcttg aataatttac gtaaaaatac gatgacataa ccagctaaga atccaccaat
    64681 cagtgcacct aagaatcctg caccgcctgt attagctaat gcaccagcag caaatccaac
    64741 gatcaaacca gggcggtcac cgatacttga agcaataaat cctgctaata ccggcagcat
    64801 gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta gcagcttgat tgtaattccc
    64861 tagatttgat aattgatcat gtggcacgcc aataaattgg tcgatcatga aagaaagcgc
    64921 aatagcgatt cctccaccaa taacgaaagg taacatgtgt gatacgccgt tcatcaaatc
    64981 tttgtaaatt ctttgtccga tagtaccatc cgcatttcct tcttctgaac tgctttcttt
    65041 tccatcacca tggaatactg gtgcagtacc actaagggct agattgatca gctcttctgg
    65101 tttacggata ccatcactga caggacgatt gatcagttct tttccgtcaa agcgattcat
    65161 ctctactttt ttgtctgctg caacgattac accttctgca cgggcgatgt cttcagcagt
    65221 caatcggttt ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca cacccatttc
    65281 tttcgctttt ttcttcagag catcttctgc catgtaagtg tgagcgatcc ctgttggaca
    65341 agctgtaacg gcaacaacga attttctttc tggatgagca gaagcttctg cagcttgtgt
    65401 ttcttgttct gctttttctt ctgcttcttt ttgctcttca gctgtttgga acaagtcttg
    65461 gacttcttct ggtgtatttg cttcttttaa ttttcctacg aaatctgcat caatcagcaa
    65521 tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg gagctgcgat
    65581 catgaaaaac agataagttg gctgtccgtc taaggcttca taatctactc ctttttggct
    65641 ttttgcgaat aaaactgttg cttctttaac tgcactgttt ttcgcgtgag gcatggcgat
    65701 tccatcacca agacctgtag aagtttgtgc ttcgcgggct agaattcctt ctttatatgt
    65761 ttcgatatcg gaaatccggc cggcatcaaa cattttttgg accatttcat cgattgcgcc
    65821 ttttttatca gtcgcttgca aatccatgat catcgcttct ttgaccatta aatccttgat
    65881 attcatgatt atcctccttg aaactaatca atttttgtga tttcaacttc tggcagcaac
    65941 tcatcaatca attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct tccgcaagca
    66001 actccccatt taaaggcttc gattggatct ttcgtacggc tgtaagcccc aataaagccg
    66061 gcaatcattg aatcccctgc acctactgaa tttttcaatg ggcgaactaa tacattcgtt
    66121 ttataagttc cttgttccgt gaagagcaaa gcaccgtctc cagccattga gatcagcaca
    66181 ttctgtgccc cttcagctag caatttttta ccatacggaa tgatttcttc tacagattgg
    66241 aaggttgtat tgtaaagctc agctaattcg tgattgtttg gtttgatcaa caatggtttc
    66301 ttaacaagtg cattttttag gtcatcacct gttgtatcaa tcacgaaatc tgcacctttt
    66361 tctcgaatga tatcaatcag ttcttgataa aatccttttc gcaggctagc cggcgcgctt
    66421 cctgacaata cgacgatatc tccttgtcct actgttccta atgcttgctt caactcatcg
    66481 atttcttcat ctgaaataga tgggcccaaa ccgttaattt cagtttcagt atctgatttt
    66541 agtttgatat tgattcttgt atcttctttt acggttgtga acaaagactg gatttgttct
    66601 ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc tagtgctgta
    66661 gattgagaat cgatccgttt caaaatacgg gaaacattga tcccttttcc tccaggtaac
    66721 ttgaagtctt ccttcattcg gttcaaatcg cctagtttta acccatctac gcgtacgata
    66781 taatcaattg atggattaag tgttactgtg taaatcattg tattgcctcc ttaattgttg
    66841 tcttttcacg gaattcatta acgaattcta atggacaacg ctcggttatg atcgtggctg
    66901 cttccaaagg tgcaacagag gtgaaggaca actggttgaa tttggtatga tcaatcaata
    66961 caaatgtctc ttcggaattt ttcatggcta aacgtttaac agctgcctct tctgggtccg
    67021 gagtcgtgaa tccttgttca agatgggcac cgttcattcc cataaatgct ttattaaaat
    67081 gaaaattttt tagttgttct atcgcattag atcctaaaac agcattagtt gccagcttta
    67141 tcgttccacc taaaataatg gtagcaagct ccaaatcaac taaatttgcc gcatgttgaa
    67201 cggaatttgt cactactgtg atctgttttc cttgcagaaa aggaatcatt tctaacgtag
    67261 tagagccagc gtctaaataa atgacatctt cgtcattaat ggtttcagct gccgatttgg
    67321 cgatttgctg tttttcgtga acgtttttga gtgatttttc actcatattc tgctcaaaat
    67381 tcaataattg ctctttctta gctccgccat gaactctttc tagaaaccca ctattctcca
    67441 gctcttgtaa atcacgtctt atcgttgatt cagaagcatt aaaaaggttt acaagatctt
    67501 gtgatttcac gattttctgc tgttcaagca agtctaggat tttttgtcta cgttcctctg
    67561 taagcatttt cacgatcacc tcaatacaaa taatagcata ttttttttct actcgcaata
    67621 taaatcagtc aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaat aaaaaaccag
    67681 tcaaaaagac
//
LOCUS       DAYYNQ010000005        58688 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000005 DAYYNQ010000000
VERSION     DAYYNQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.005"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1462)
                     /locus_tag="ACHBJ1_000294"
     CDS             complement(176..1462)
                     /locus_tag="ACHBJ1_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9429269.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1455..2723)
                     /locus_tag="ACHBJ1_000295"
     CDS             complement(1455..2723)
                     /locus_tag="ACHBJ1_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM9429270.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2975..3436
                     /gene="mscL"
                     /locus_tag="ACHBJ1_000296"
     CDS             2975..3436
                     /gene="mscL"
                     /locus_tag="ACHBJ1_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM9429271.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3495..3941)
                     /locus_tag="ACHBJ1_000297"
     CDS             complement(3495..3941)
                     /locus_tag="ACHBJ1_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429272.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4034..4279)
                     /locus_tag="ACHBJ1_000298"
     CDS             complement(4034..4279)
                     /locus_tag="ACHBJ1_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM9429273.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4394..4972)
                     /locus_tag="ACHBJ1_000299"
     CDS             complement(4394..4972)
                     /locus_tag="ACHBJ1_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9429274.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5049..5579)
                     /locus_tag="ACHBJ1_000300"
     CDS             complement(5049..5579)
                     /locus_tag="ACHBJ1_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM9429275.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACHBJ1_000301"
     CDS             complement(5583..6443)
                     /gene="rlmB"
                     /locus_tag="ACHBJ1_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM9429276.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6436..6831)
                     /locus_tag="ACHBJ1_000302"
     CDS             complement(6436..6831)
                     /locus_tag="ACHBJ1_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM9429277.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACHBJ1_000303"
     CDS             complement(6828..8240)
                     /gene="cysS"
                     /locus_tag="ACHBJ1_000303"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM9429278.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACHBJ1_000304"
     CDS             complement(8237..8773)
                     /gene="epsC"
                     /locus_tag="ACHBJ1_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM9429279.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8859..9098)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACHBJ1_000305"
     CDS             complement(9095..10555)
                     /gene="gltX"
                     /locus_tag="ACHBJ1_000305"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM9429280.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10725..11882)
                     /locus_tag="ACHBJ1_000306"
     CDS             complement(10725..11882)
                     /locus_tag="ACHBJ1_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM9429281.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACHBJ1_000307"
     CDS             complement(11916..13286)
                     /gene="radA"
                     /locus_tag="ACHBJ1_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM9429282.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13349..13876)
                     /locus_tag="ACHBJ1_000308"
     CDS             complement(13349..13876)
                     /locus_tag="ACHBJ1_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM9429283.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14106..14912
                     /gene="proB"
                     /locus_tag="ACHBJ1_000309"
     CDS             14106..14912
                     /gene="proB"
                     /locus_tag="ACHBJ1_000309"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM9429284.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14909..16156
                     /locus_tag="ACHBJ1_000310"
     CDS             14909..16156
                     /locus_tag="ACHBJ1_000310"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM9429285.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16232..16576
                     /locus_tag="ACHBJ1_000311"
     CDS             16232..16576
                     /locus_tag="ACHBJ1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429286.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16693..17019)
                     /locus_tag="ACHBJ1_000312"
     CDS             complement(16693..17019)
                     /locus_tag="ACHBJ1_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429287.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17634..18836)
                     /locus_tag="ACHBJ1_000313"
     CDS             complement(17634..18836)
                     /locus_tag="ACHBJ1_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429288.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            18953..19468
                     /locus_tag="ACHBJ1_000314"
     CDS             18953..19468
                     /locus_tag="ACHBJ1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9429289.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19667..20113)
                     /locus_tag="ACHBJ1_000315"
     CDS             complement(19667..20113)
                     /locus_tag="ACHBJ1_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9429290.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACHBJ1_000316"
     CDS             complement(20160..21638)
                     /gene="msr(C)"
                     /locus_tag="ACHBJ1_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9429291.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21992..22621)
                     /locus_tag="ACHBJ1_000317"
     CDS             complement(21992..22621)
                     /locus_tag="ACHBJ1_000317"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9429292.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22605..23069)
                     /locus_tag="ACHBJ1_000318"
     CDS             complement(22605..23069)
                     /locus_tag="ACHBJ1_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9429293.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23556..24824)
                     /locus_tag="ACHBJ1_000319"
     CDS             complement(23556..24824)
                     /locus_tag="ACHBJ1_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9429294.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25055..25792)
                     /locus_tag="ACHBJ1_000320"
     CDS             complement(25055..25792)
                     /locus_tag="ACHBJ1_000320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9429295.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25859..26428)
                     /locus_tag="ACHBJ1_000321"
     CDS             complement(25859..26428)
                     /locus_tag="ACHBJ1_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9429296.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26556..27428
                     /locus_tag="ACHBJ1_000322"
     CDS             26556..27428
                     /locus_tag="ACHBJ1_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9429297.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27770..28642
                     /locus_tag="ACHBJ1_000323"
     CDS             27770..28642
                     /locus_tag="ACHBJ1_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9429298.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28639..30597
                     /gene="nagE"
                     /locus_tag="ACHBJ1_000324"
     CDS             28639..30597
                     /gene="nagE"
                     /locus_tag="ACHBJ1_000324"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM9429299.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30929..31780)
                     /locus_tag="ACHBJ1_000325"
     CDS             complement(30929..31780)
                     /locus_tag="ACHBJ1_000325"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9429300.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31903..32370
                     /locus_tag="ACHBJ1_000326"
     CDS             31903..32370
                     /locus_tag="ACHBJ1_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM9429301.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32633..33115)
                     /locus_tag="ACHBJ1_000327"
     CDS             complement(32633..33115)
                     /locus_tag="ACHBJ1_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9429302.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33215..34078)
                     /locus_tag="ACHBJ1_000328"
     CDS             complement(33215..34078)
                     /locus_tag="ACHBJ1_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9429303.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            34676..35551
                     /locus_tag="ACHBJ1_000329"
     CDS             34676..35551
                     /locus_tag="ACHBJ1_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM9429304.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(35696..35848)
                     /locus_tag="ACHBJ1_000330"
                     /pseudo
     CDS             complement(35696..35848)
                     /locus_tag="ACHBJ1_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(36116..37447)
                     /locus_tag="ACHBJ1_000331"
     CDS             complement(36116..37447)
                     /locus_tag="ACHBJ1_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9429305.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(37596..38309)
                     /locus_tag="ACHBJ1_000332"
     CDS             complement(37596..38309)
                     /locus_tag="ACHBJ1_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9429306.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            38484..39908
                     /locus_tag="ACHBJ1_000333"
     CDS             38484..39908
                     /locus_tag="ACHBJ1_000333"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9429307.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            40207..40914
                     /locus_tag="ACHBJ1_000334"
     CDS             40207..40914
                     /locus_tag="ACHBJ1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9429308.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            40901..41224
                     /locus_tag="ACHBJ1_000335"
     CDS             40901..41224
                     /locus_tag="ACHBJ1_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9429309.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(41313..42350)
                     /locus_tag="ACHBJ1_000336"
     CDS             complement(41313..42350)
                     /locus_tag="ACHBJ1_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9429310.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(42671..43654)
                     /locus_tag="ACHBJ1_000337"
     CDS             complement(42671..43654)
                     /locus_tag="ACHBJ1_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM9429311.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(44069..44839)
                     /locus_tag="ACHBJ1_000338"
     CDS             complement(44069..44839)
                     /locus_tag="ACHBJ1_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429312.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(44832..45767)
                     /locus_tag="ACHBJ1_000339"
     CDS             complement(44832..45767)
                     /locus_tag="ACHBJ1_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429313.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(46083..47732)
                     /locus_tag="ACHBJ1_000340"
     CDS             complement(46083..47732)
                     /locus_tag="ACHBJ1_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9429314.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(47849..50452)
                     /locus_tag="ACHBJ1_000341"
     CDS             complement(47849..50452)
                     /locus_tag="ACHBJ1_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9429315.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(50464..51513)
                     /locus_tag="ACHBJ1_000342"
     CDS             complement(50464..51513)
                     /locus_tag="ACHBJ1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9429316.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(51539..51853)
                     /locus_tag="ACHBJ1_000343"
     CDS             complement(51539..51853)
                     /locus_tag="ACHBJ1_000343"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM9429317.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(51869..52315)
                     /locus_tag="ACHBJ1_000344"
     CDS             complement(51869..52315)
                     /locus_tag="ACHBJ1_000344"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM9429318.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(52316..54238)
                     /locus_tag="ACHBJ1_000345"
     CDS             complement(52316..54238)
                     /locus_tag="ACHBJ1_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9429319.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(54450..55346)
                     /locus_tag="ACHBJ1_000346"
     CDS             complement(54450..55346)
                     /locus_tag="ACHBJ1_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9429320.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(55339..56019)
                     /locus_tag="ACHBJ1_000347"
     CDS             complement(55339..56019)
                     /locus_tag="ACHBJ1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9429321.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(56020..56802)
                     /locus_tag="ACHBJ1_000348"
     CDS             complement(56020..56802)
                     /locus_tag="ACHBJ1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9429322.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(56795..57712)
                     /locus_tag="ACHBJ1_000349"
     CDS             complement(56795..57712)
                     /locus_tag="ACHBJ1_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9429323.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(57876..58130)
                     /locus_tag="ACHBJ1_000350"
     CDS             complement(57876..58130)
                     /locus_tag="ACHBJ1_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429324.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT        17197 a        12175 c        10197 g        19119 t
ORIGIN      
        1 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
       61 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
      121 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
      181 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
      241 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
      301 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
      361 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
      421 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
      481 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
      541 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
      601 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
      661 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
      721 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
      781 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
      841 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
      901 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
      961 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     1021 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     1081 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     1141 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     1201 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     1261 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     1321 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     1381 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     1441 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     1501 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     1561 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     1621 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     1681 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     1741 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     1801 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     1861 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     1921 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     1981 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     2041 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     2101 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     2161 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     2221 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     2281 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     2341 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     2401 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     2461 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     2521 taaagctctg caagttgcgc acttaacttt gtctgatccg aataattcaa actatttgtt
     2581 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     2641 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     2701 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     2761 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     2821 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     2881 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     2941 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     3001 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
     3061 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
     3121 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
     3181 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
     3241 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
     3301 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
     3361 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
     3421 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
     3481 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
     3541 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
     3601 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
     3661 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
     3721 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
     3781 tcagtatctt gaaaaaacgt atgccaagct tctccatcaa tgtcatcaac ttcttgataa
     3841 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
     3901 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
     3961 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
     4021 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
     4081 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
     4141 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
     4201 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
     4261 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
     4321 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
     4381 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
     4441 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
     4501 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
     4561 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aattgctaaa ggttcttgca
     4621 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
     4681 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
     4741 tgcttataac gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
     4801 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
     4861 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
     4921 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
     4981 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
     5041 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
     5101 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
     5161 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
     5221 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
     5281 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
     5341 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
     5401 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
     5461 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
     5521 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
     5581 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
     5641 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
     5701 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
     5761 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
     5821 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
     5881 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
     5941 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
     6001 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
     6061 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
     6121 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
     6181 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
     6241 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
     6301 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
     6361 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
     6421 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
     6481 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
     6541 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
     6601 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
     6661 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
     6721 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
     6781 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
     6841 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
     6901 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
     6961 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
     7021 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
     7081 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
     7141 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
     7201 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
     7261 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
     7321 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
     7381 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
     7441 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
     7501 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
     7561 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
     7621 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
     7681 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
     7741 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
     7801 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
     7861 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
     7921 ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
     7981 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
     8041 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
     8101 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
     8161 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
     8221 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
     8281 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
     8341 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
     8401 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
     8461 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
     8521 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
     8581 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
     8641 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
     8701 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
     8761 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
     8821 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
     8881 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
     8941 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
     9001 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
     9061 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
     9121 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
     9181 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
     9241 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
     9301 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
     9361 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
     9421 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
     9481 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
     9541 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
     9601 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
     9661 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
     9721 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
     9781 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
     9841 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
     9901 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
     9961 caaaattata agttggcata ccatcgcgtt tttggatgac aaagtcgcca ccgatattat
    10021 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    10081 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    10141 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    10201 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    10261 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    10321 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    10381 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    10441 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    10501 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    10561 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    10621 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    10681 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    10741 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    10801 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    10861 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    10921 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    10981 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    11041 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    11101 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    11161 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    11221 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    11281 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    11341 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    11401 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    11461 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    11521 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    11581 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    11641 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    11701 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    11761 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    11821 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    11881 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    11941 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    12001 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    12061 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    12121 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    12181 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    12241 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    12301 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    12361 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    12421 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    12481 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    12541 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    12601 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    12661 atcccatttg ttttagcggt tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    12721 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    12781 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    12841 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    12901 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    12961 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    13021 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    13081 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    13141 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    13201 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    13261 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    13321 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    13381 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    13441 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    13501 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    13561 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    13621 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    13681 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    13741 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    13801 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    13861 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    13921 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    13981 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    14041 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    14101 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    14161 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    14221 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    14281 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    14341 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    14401 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    14461 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    14521 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    14581 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    14641 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    14701 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    14761 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    14821 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    14881 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    14941 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    15001 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    15061 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    15121 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    15181 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    15241 cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    15301 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    15361 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    15421 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    15481 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    15541 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    15601 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    15661 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    15721 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    15781 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    15841 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    15901 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    15961 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    16021 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    16081 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    16141 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    16201 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    16261 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    16321 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    16381 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    16441 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    16501 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    16561 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    16621 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    16681 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    16741 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    16801 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    16861 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    16921 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    16981 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    17041 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    17101 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    17161 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    17221 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaat
    17281 cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat gagaagctat ttttccgttt
    17341 tgtttctcta atacttagga taaaacgctg attttacaac ctctgttaaa cggtgttcaa
    17401 tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt
    17461 tgtttctcta atactcagga caaaacgctg attttacaac ctcctgataa acggtgttca
    17521 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgagaag ctatttttgt
    17581 ttggctgttc ttatgacttg atgcaggaca gctttttcac aacctctgat ttcttattct
    17641 tcttcatttg tagcaaaata attacggtga taaatgatac aacatacact gataagaaca
    17701 atggcacctg caaaataaaa cgcatagctt tcaccaatat gttcaccaat cacacctgca
    17761 ataaatggtg ctaaagctcc accgaaaaag cggacaaaac tatagctgga cgaagcaacg
    17821 tttcgttgca gacctggtat ttccatagca atcgtcgtca acagtgtatt gataattcct
    17881 tggaaaaatc ctgctaacac aatgctgatc ggtacaagga ctggcataga agttccagct
    17941 cctaataaca gcaaacaaat aatgaataag acaaatgctg ctaaaattgt ccgataagtc
    18001 gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag caatagccag aagcacaccc
    18061 cagccaaaaa atacaaaacc gacttctaat tcagaatagc caatcaataa aaatggcgca
    18121 tacgcaagta aagtaaagaa tccgaaatta tataacaatg cgattagtcc aactgaacga
    18181 agtttatgat tgcttaacgc tttgacacct tcccaaaggc ctacttttct ctttggtttc
    18241 tcagtttcag gtaaaagtac cgctagggct ataaaaccgc aaaacatcaa aaatgctacc
    18301 ccaaaaaatg gataacgcca tgaaccactg cccaagaccc caccgactag agggccaaca
    18361 gccattccgc agccaagtgc tgcttcatac atcatgattg ctttttctgt gttaccaact
    18421 aaaatactga cgatcgttgc taaagcagta gagacaaata aagcattccc gacaccccag
    18481 ccggcacgta acccaatcag cattccgata ttcccagata atccgccaag accagcgaat
    18541 acaacgatga taaacaatcc tgtcatcaat gtttttttca aaccgatccg actagataag
    18601 aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa taacagggtc
    18661 gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg gtctactaga
    18721 cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga acttttcccc
    18781 tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat ttccatccct
    18841 ttctctctat tagcatcata catgatttat tttacatgtc aatatgcaca tgtaaaacgt
    18901 ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt ttatgaatac
    18961 tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt atgaattgaa
    19021 gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa cgcttgccaa
    19081 gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc aaaaaaagat
    19141 ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag aggaaactcc
    19201 cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga cgctcgataa
    19261 aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa tcatgcaaaa
    19321 aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg gacgttatgt
    19381 tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc agcaagtaag
    19441 agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag aaatatctat
    19501 tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa agcgatccga
    19561 acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat tcaagagact
    19621 atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat tcattacttt
    19681 gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc aacggtaaag
    19741 cggcaatcac attcattttc caagcattca tataagcaga gaataattga tcaggtattc
    19801 cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa accatcaagc
    19861 atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt ttaaaggcaa
    19921 tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc acgaatccag
    19981 gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat aaattatacg
    20041 cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt ccttcttttt
    20101 tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc gtagcacatt
    20161 taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata aacttcatca
    20221 gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc cggatatatt
    20281 tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt cggttcatct
    20341 aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg ggtcgcttct
    20401 cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc taagtgattc
    20461 agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa acgaatgatg
    20521 gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct tactttcgga
    20581 gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt cttacctgaa
    20641 ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc accatttacc
    20701 agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg atagcggctg
    20761 tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg cggcttggtt
    20821 gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg gatggctttt
    20881 tgtacactgt ctttttgttt agaggaagac aaccgatcca gtctgatttg ttgctttttc
    20941 tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg gatcgatttt
    21001 tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt ctttttctcc
    21061 aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat ttctccgtct
    21121 tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg actaacaaaa
    21181 accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact ttttctatct
    21241 agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag aagacgtatc
    21301 aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc ttccagtctt
    21361 aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag ataggttaat
    21421 tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg aaaaagttca
    21481 ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat gatccctata
    21541 cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt gccaaagctg
    21601 acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact cctatttcat
    21661 gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca aaaaaactgc
    21721 atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa tctgtcatag
    21781 ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg aaattgttgt
    21841 ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg taatttcatc
    21901 gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat tcaaaaaaag
    21961 caatagctac tgaatagtaa ctgaaaacct tttattcgaa catagtgatc ttttctggtg
    22021 ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa ataataactg
    22081 gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct tcgaatccac
    22141 caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc atagccgcta
    22201 agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc ttgtaataag
    22261 gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct gattcgtaag
    22321 cttggttgta gatttcttcg ccatattcgt aacattgcat atcgccaaaa atcatgacca
    22381 ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc aagatttctt
    22441 ttgcttcctc gctttcagcg accacaaagc gccatggttt catattgaca gaagaaggtg
    22501 cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg tatgtttcat
    22561 catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt ttttgttcca
    22621 ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt caatttatct
    22681 aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag cacggaacct
    22741 gtatctttat ggtccatttc acaatgcttg attttcttct gggctttttc tgttagcgct
    22801 acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc atttgcttcc
    22861 aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc ttgcaaactg
    22921 ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct agtaagactt
    22981 ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt gatttgatat
    23041 aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat tgactcgtca
    23101 acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc agctgtgtaa
    23161 taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta gaatgaacgg
    23221 aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta tcaaactata
    23281 gaagatagct cttctgcagt agataatttt acaccgccaa aaatttccga aatcgccttt
    23341 tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt aataaaaaag
    23401 ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa cttgcatctg
    23461 cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg ctgttcttac
    23521 tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa tcctattcct
    23581 tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa ggtatagtga
    23641 tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga accaccagaa
    23701 taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat ctcagattgt
    23761 gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac aggttgtccg
    23821 ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg cagataattt
    23881 ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata cagacgatac
    23941 atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt ttcgattaca
    24001 ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag ccaagccgta
    24061 gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta aacgcctggc
    24121 tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt gtacgaagga
    24181 tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc cgaagtaggc
    24241 atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta attaggggca
    24301 gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat catgactgat
    24361 gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact atcaatgaac
    24421 gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata gttttcgaat
    24481 tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa ttggttcgtg
    24541 atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc ttcggtttgt
    24601 gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc tgagacagta
    24661 gctgactgca aagtactttc tgtatcgttt tcaattcctg tttctgaaga actcgatgtc
    24721 tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc aactgctaga
    24781 tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt tctcctttga
    24841 attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag tcaatgtcca
    24901 tatttaaata acatttgttt ctttttaata aatttgcttt ttctgcataa aaagacgtaa
    24961 atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat aactatttaa
    25021 caagtaattc tctctggata ttatccgtta tggattatac ttcctgtgtc gtcggacgga
    25081 taatcatgtt gctgacagac atccggtctg gcgtttctat cgcaaatgct acagcagaag
    25141 ctatatcttc agatgttaat ccccattctt tttgcgcttc atgaagttca agagcagcct
    25201 ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag atgatcgtag
    25261 atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca cggacagcaa
    25321 attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc acagaatcag
    25381 ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg gaaattccat
    25441 ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg tgaccttcaa
    25501 ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt cgaccgaact
    25561 tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca tctgctgttt
    25621 gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga tcttctcggc
    25681 gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt gcttcaccga
    25741 ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc attctattct
    25801 cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat cttactgttc
    25861 aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg atggtattgt
    25921 ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg ccaatcgtta
    25981 cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg tggattggct
    26041 ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg atgaggttga
    26101 cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca acgaacatca
    26161 cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa tctgtataaa
    26221 aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt tctagccgtt
    26281 tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc tcttcgtttt
    26341 ccgcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc tctttattga
    26401 cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt actttataac
    26461 ggacggctta tgatgtctaa tacttataaa acatacttaa ctattccttt taactatact
    26521 tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata ctgacgtatt
    26581 ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg catatcacgc
    26641 aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg acacttttca
    26701 ctcgaggctc ccgagagata cagttgacgg attctggtcg atatctttat tcaaaaggaa
    26761 aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac gcaatcatct
    26821 ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt tctgatagcc
    26881 ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc gggaatgcag
    26941 atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta atcgaaccga
    27001 caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg ggacttttga
    27061 tacatcggaa tcatccttta gcaaagcaaa cagtcatcca tccgcaggaa ctgaaaactg
    27121 ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa tggctaggtt
    27181 tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat gcttctttga
    27241 tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat acttatggga
    27301 cggaaaaagt attcatccca ctcgatccgc cattacaagc ttcactgaat ctcatttgga
    27361 aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta acggagaaat
    27421 acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat aagttaagca
    27481 aaagatccaa tgattaaata acataagttt cttaatttgt tccttccttt tatttctgat
    27541 aattccagta aattactata attataaaaa aagaattgac aaaacataac tgtctatata
    27601 aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag gcatgaaaaa
    27661 tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt tggccgcaat
    27721 caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca tgaaaatcaa
    27781 aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa aagtagctat
    27841 tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc gtgatgtcga
    27901 acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac ttaatcagat
    27961 tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa ctgttttgat
    28021 ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt tttctgcgga
    28081 gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg aagtgcttta
    28141 cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag agcttgggat
    28201 tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg gacgaagcgg
    28261 tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca tcatccattt
    28321 agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga attattcaag
    28381 actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat atgctgtttt
    28441 agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata agatttctaa
    28501 aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag aagaactggg
    28561 ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa atgttaacaa
    28621 tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc ccttcaactt
    28681 ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg gtgggcatct
    28741 tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt tttaggtcaa
    28801 ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa atcaggtgct
    28861 gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa aactgattca
    28921 gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc tcagatcagt
    28981 aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa tcgtttccac
    29041 gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt gccaatcttg
    29101 gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc tccggtttac
    29161 tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg tgcaggtctt
    29221 tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat gaactccgtg
    29281 ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc tgaaggaaca
    29341 aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt cgggcttcca
    29401 gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa aacagcttcc
    29461 ttgatgttag cagcagcttt tgcttcattc tttactggtg taacagaacc acttgaattc
    29521 tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac tgggatttct
    29581 ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc cggatttgtc
    29641 gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt gatcgtccaa
    29701 ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa aaaattccat
    29761 ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc aacgactaat
    29821 tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg cggagccgac
    29881 aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa agatacaggc
    29941 ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat catcgacgga
    30001 aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga gatggcaaaa
    30061 ctacatggtg gtgctgcaac acgacctgca caaacaaaca ccgtggttaa aacagaaaca
    30121 tttgccggtg gaacgatcga acaagatatt tacgctatcg caaatgggaa actgattcca
    30181 atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg ctatgctgta
    30241 ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa cattttccca
    30301 acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct tcatatgggc
    30361 atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtaaaaga aggtcaaaaa
    30421 gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc tgcaggcaaa
    30481 aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt agaaatcgaa
    30541 cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa agcctgagcc
    30601 atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa aaatcttgtc
    30661 tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt aataagtcca
    30721 gccaaacgta acatatgaag agctattttt tatttgactc ttcttattac ttgatgcaga
    30781 acagcttttt tacaacctct gataaacggt gttcaaaaac cagtttatcg gtatgaagtc
    30841 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttcata ctcaaaacta
    30901 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    30961 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    31021 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    31081 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    31141 ttttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    31201 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    31261 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    31321 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    31381 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    31441 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    31501 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    31561 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    31621 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    31681 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    31741 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    31801 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    31861 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    31921 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    31981 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    32041 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    32101 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    32161 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    32221 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    32281 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    32341 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    32401 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    32461 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    32521 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    32581 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    32641 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    32701 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    32761 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgattacaa
    32821 caagatacgt tccttttggg aatgggatga ttcgggtagt gtttggaaca tcctgactcg
    32881 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    32941 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    33001 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    33061 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    33121 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    33181 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    33241 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    33301 cttcgactaa ctcataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    33361 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    33421 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    33481 gcatcaaact gcccttttct tccaagcggg ctccatcaaa tccataagaa acgttgatta
    33541 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    33601 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    33661 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    33721 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctgctga
    33781 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    33841 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctaggaga tcgcgattag
    33901 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    33961 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    34021 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    34081 ttatcttcct ccttgcttgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    34141 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    34201 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    34261 ataaattttg gggaagacac attctaagta aacaaagcgg taaaaatatc ttttattccc
    34321 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    34381 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    34441 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    34501 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    34561 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    34621 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    34681 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    34741 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    34801 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    34861 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    34921 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    34981 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcaatttat
    35041 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    35101 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    35161 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgctcattc agcaaaatgc
    35221 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    35281 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    35341 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    35401 agtcgatcat actgacgcct cttattcaaa acgctactcc ataaaaatcc tgaaagaaga
    35461 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaaa ctaaagaaac
    35521 ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac gataacaacc atcctaaatc
    35581 cacaagaaat acagcagacc ttgctttaaa aacacgaatc atgcaaagga tgaagtaaag
    35641 aagacttaac gcgtacccaa tattagtcat ttgactatta ggtacgcgtt ttgttttata
    35701 agtcatgaca aatttccttc gttgctgggt agattttccg gcattgttga tagattttat
    35761 gatacttttc tacattttgt aagagaggtt ccactgcatc tgtatattgg ataaaattct
    35821 gacaacaact ttcaggtgtg tcgaaccact ctaatcttta aagataaata ttaaaataac
    35881 gattaataat attaacagtc tttatttttc accaccaaaa acctatcttt gcatatacta
    35941 aatagacgta tatacacaga taggtttgtc ttgtgaaaaa taatgattat ccttaccaaa
    36001 aaatgtttgt ttttctacat gttcgtactt atcaaaaaat atgatcctta ttgaaattac
    36061 tggctattta tattccaacg acttcttgtg ttaatttttg ttatctcata gtaaattatg
    36121 tttcattttc acttaattta gttgtcacgt gttcgtcaat tcgtaaaaac ggtaaatata
    36181 taaacacact tgcggctata attaatattt gtaaaacagc tgctcgccag tctccggctg
    36241 ttgctaaaaa tcccgatata actggtggcg tagtccaagg aatttgcact actacatgat
    36301 taattaatcc cacagcagtt gcaaaatatg ctactgtcaa agaaaaaatc ggtgcaagca
    36361 caaagggaat aatcagactg atattaaata caataggcaa tccaaaaata ataggttcgc
    36421 ttatattgaa tatagcggga gttaccgata atttagcaaa gtccttataa tcttttctac
    36481 gactgaagat aaatattgca atcaataaag caatcgtatt tcccgatcca cccattaccg
    36541 caaaggtatc tttaaaggcc atattgataa tatgtggaat ttgctctttg tttgcataag
    36601 ctgccatatt ttcttgcata ttctgaatca aaaacggatc taacaatgaa cctgatatta
    36661 ctgcttgatg gataccaaat ccaaaaaata gattagcaat agatgtaatt aacaaaaatc
    36721 ctggtaaacc agttgtaatt ttacttaatg gctctgtaac tatcgtggta attagcttat
    36781 ttatatccat attaaacaat tgaatgatta taaatgacaa taaagcaaac gaggtaatcg
    36841 taataatgat tgggattaat acattaaaag actttaaaac agcgggagga atatttccgg
    36901 atagctgaat ctgtagtttc ttattattag aaagtttaat aaataattct gtagccgcta
    36961 atccaacaaa tatacctaca aacattcctg aagctcctgt ataggatata ggtatcacac
    37021 ccgtcacttc aacagcttta tccgcatttt caggagtaaa catcatctgc ataggtgtta
    37081 atacaacaaa tgtcgaaata gcaactaata ccgctgcaat atgatttgta taatttctgc
    37141 ttgcacacat atgataagat actgctgcta caactaataa tgtagttatg cttaatgtac
    37201 cattggcaat tgaagtacca atttgttgta acgtggtcaa tgtactagta tcgacaattt
    37261 cccccatgaa tcctgttggt tctaagatca ctgaattaat aagtgtcaca aatcctgcta
    37321 acatcataaa tggcataaat gtagcaaacg catctcgcaa agttcttaaa tggatctgat
    37381 tccccaactt agtcgcaact ataccaaatt tttcaaatac aatgtccttc ctgctacttt
    37441 tgcccatatt ttttctcctt tatgcttatt gtaatcgatt actttttcca cccaaataat
    37501 acatcactta tcaaaaaaag taaatacttt taattaaata tatatatctt ttttagtata
    37561 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
    37621 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
    37681 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
    37741 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
    37801 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
    37861 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
    37921 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
    37981 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
    38041 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
    38101 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
    38161 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
    38221 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
    38281 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
    38341 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
    38401 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
    38461 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
    38521 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
    38581 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
    38641 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
    38701 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
    38761 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
    38821 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
    38881 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
    38941 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
    39001 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
    39061 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
    39121 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
    39181 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
    39241 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
    39301 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
    39361 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
    39421 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
    39481 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
    39541 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
    39601 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
    39661 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
    39721 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
    39781 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
    39841 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
    39901 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
    39961 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
    40021 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
    40081 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
    40141 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
    40201 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
    40261 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
    40321 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
    40381 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
    40441 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
    40501 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
    40561 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
    40621 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
    40681 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
    40741 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
    40801 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
    40861 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
    40921 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
    40981 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
    41041 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
    41101 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
    41161 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
    41221 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
    41281 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
    41341 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
    41401 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
    41461 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
    41521 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
    41581 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
    41641 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
    41701 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
    41761 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
    41821 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
    41881 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
    41941 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
    42001 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
    42061 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
    42121 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
    42181 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
    42241 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
    42301 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
    42361 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
    42421 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
    42481 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
    42541 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
    42601 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
    42661 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
    42721 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    42781 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    42841 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    42901 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
    42961 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
    43021 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
    43081 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    43141 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    43201 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
    43261 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
    43321 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    43381 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    43441 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
    43501 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    43561 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    43621 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    43681 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    43741 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
    43801 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
    43861 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    43921 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    43981 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    44041 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    44101 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
    44161 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    44221 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
    44281 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
    44341 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
    44401 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    44461 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
    44521 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
    44581 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
    44641 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
    44701 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
    44761 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    44821 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
    44881 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    44941 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    45001 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
    45061 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
    45121 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
    45181 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
    45241 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
    45301 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    45361 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    45421 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    45481 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
    45541 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
    45601 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
    45661 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
    45721 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    45781 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
    45841 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
    45901 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
    45961 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    46021 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
    46081 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
    46141 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
    46201 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
    46261 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
    46321 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
    46381 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
    46441 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
    46501 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
    46561 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
    46621 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
    46681 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
    46741 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
    46801 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
    46861 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
    46921 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
    46981 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
    47041 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
    47101 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
    47161 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
    47221 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
    47281 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
    47341 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
    47401 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
    47461 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
    47521 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    47581 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    47641 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    47701 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    47761 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    47821 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    47881 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    47941 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    48001 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    48061 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    48121 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    48181 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    48241 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    48301 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    48361 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    48421 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    48481 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    48541 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    48601 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    48661 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    48721 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    48781 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    48841 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    48901 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    48961 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    49021 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    49081 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    49141 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    49201 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    49261 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    49321 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    49381 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    49441 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    49501 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    49561 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    49621 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    49681 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    49741 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    49801 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    49861 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    49921 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    49981 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    50041 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    50101 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    50161 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    50221 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    50281 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    50341 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    50401 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    50461 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    50521 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    50581 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    50641 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    50701 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    50761 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    50821 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    50881 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    50941 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    51001 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    51061 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    51121 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    51181 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    51241 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    51301 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    51361 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    51421 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    51481 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    51541 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    51601 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    51661 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    51721 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    51781 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    51841 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    51901 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    51961 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    52021 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    52081 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    52141 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    52201 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    52261 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    52321 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    52381 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    52441 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    52501 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    52561 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    52621 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    52681 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    52741 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    52801 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    52861 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    52921 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    52981 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    53041 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    53101 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    53161 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    53221 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
    53281 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
    53341 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
    53401 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    53461 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    53521 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    53581 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    53641 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    53701 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    53761 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    53821 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    53881 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    53941 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    54001 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    54061 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    54121 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    54181 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    54241 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    54301 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag
    54361 cctgcaatat ttatatcaca ggcttttttc tctattaaat atatataaga taatacattt
    54421 ttctactagt tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag
    54481 aagatgcccc cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt
    54541 gacaggtaaa gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa
    54601 aaacgctgga agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact
    54661 gttaatcgaa tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa
    54721 cgcaataaat tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat
    54781 acagttgtat ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt
    54841 tcataaaaag agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc
    54901 atttcttttt gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt
    54961 aatcttgcaa cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct
    55021 acctgttcca gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga
    55081 gaaaggccga cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt
    55141 tgttcatttt catccaacca tgtattcagt gcatctacta ggtccattag attcttttct
    55201 cgaaattcga ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc
    55261 ttagttaatc tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca
    55321 acgagaagca acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata
    55381 cacagcatgt gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt
    55441 ctcactatca aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac
    55501 ttttttcgga tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac
    55561 agccacacca ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata
    55621 gatgcgttgc gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat
    55681 tcgtaacaaa agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc
    55741 caacaaggaa actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt
    55801 cgatgtctgt cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc
    55861 tagaaggatc aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata
    55921 agcatcaaga agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc
    55981 atcgtcttcg ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg
    56041 gaaaataaac attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat
    56101 tttcggtgct tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc
    56161 cgtaaaccac tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa
    56221 ggccgttccc atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat
    56281 gaatgtcaaa gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa
    56341 tacttttaga acataatttc catagttttt gattccctca acttcaccaa atccgaaagc
    56401 aagtgtgtaa gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa
    56461 aagcgtaacg agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt
    56521 attgcgaatc gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa
    56581 agccagaaga atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat
    56641 gactgtatcc gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt
    56701 ccaagaataa aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct
    56761 cttccatact tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta
    56821 attgtaagaa ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt
    56881 ttgcttctac caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga
    56941 taacacggct agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg
    57001 cttgttccgt cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt
    57061 ctttcgataa ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct
    57121 gtgacaattc atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca
    57181 aacgttggat gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt
    57241 catccaaaat caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc
    57301 gcatacctaa agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt
    57361 tcagcaggtc ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt
    57421 tatctcttgc agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc
    57481 gtcttgctgc ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga
    57541 tcaaacccat aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc
    57601 catagatcat tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt
    57661 attttttggt tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc
    57721 ttctttctta ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt
    57781 ataaagaaag tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc
    57841 tttcctatta tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg
    57901 accaaagtcg tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc
    57961 ttgagtaatt ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa
    58021 attagactgt ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt
    58081 gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct
    58141 tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt
    58201 aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta
    58261 ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt
    58321 ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg
    58381 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa
    58441 taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat
    58501 agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg
    58561 tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta
    58621 cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa
    58681 gctttacg
//
LOCUS       DAYYNQ010000006        53873 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000006 DAYYNQ010000000
VERSION     DAYYNQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.006"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..1668
                     /locus_tag="ACHBJ1_000351"
     CDS             37..1668
                     /locus_tag="ACHBJ1_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM9429325.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1730..2371)
                     /locus_tag="ACHBJ1_000352"
     CDS             complement(1730..2371)
                     /locus_tag="ACHBJ1_000352"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9429326.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2557..2633
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2653..2961
                     /gene="rplU"
                     /locus_tag="ACHBJ1_000353"
     CDS             2653..2961
                     /gene="rplU"
                     /locus_tag="ACHBJ1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM9429327.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2988..3332
                     /locus_tag="ACHBJ1_000354"
     CDS             2988..3332
                     /locus_tag="ACHBJ1_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM9429328.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3345..3638
                     /gene="rpmA"
                     /locus_tag="ACHBJ1_000355"
     CDS             3345..3638
                     /gene="rpmA"
                     /locus_tag="ACHBJ1_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM9429329.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4072..6879
                     /locus_tag="ACHBJ1_000356"
     CDS             4072..6879
                     /locus_tag="ACHBJ1_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9429330.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7070..7561
                     /locus_tag="ACHBJ1_000357"
     CDS             7070..7561
                     /locus_tag="ACHBJ1_000357"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9429331.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7664..8653
                     /locus_tag="ACHBJ1_000358"
     CDS             7664..8653
                     /locus_tag="ACHBJ1_000358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM9429332.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8686..9489
                     /locus_tag="ACHBJ1_000359"
     CDS             8686..9489
                     /locus_tag="ACHBJ1_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM9429333.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9510..10424
                     /locus_tag="ACHBJ1_000360"
     CDS             9510..10424
                     /locus_tag="ACHBJ1_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9429334.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10507..10881
                     /locus_tag="ACHBJ1_000361"
     CDS             10507..10881
                     /locus_tag="ACHBJ1_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM9429335.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11020..11265
                     /locus_tag="ACHBJ1_000362"
     CDS             11020..11265
                     /locus_tag="ACHBJ1_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9429336.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11437..12501
                     /locus_tag="ACHBJ1_000363"
     CDS             11437..12501
                     /locus_tag="ACHBJ1_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM9429337.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12747..13172
                     /locus_tag="ACHBJ1_000364"
     CDS             12747..13172
                     /locus_tag="ACHBJ1_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9429338.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13165..13614
                     /gene="nusB"
                     /locus_tag="ACHBJ1_000365"
     CDS             13165..13614
                     /gene="nusB"
                     /locus_tag="ACHBJ1_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM9429339.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13711..14565
                     /locus_tag="ACHBJ1_000366"
     CDS             13711..14565
                     /locus_tag="ACHBJ1_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM9429340.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14566..15903
                     /gene="xseA"
                     /locus_tag="ACHBJ1_000367"
     CDS             14566..15903
                     /gene="xseA"
                     /locus_tag="ACHBJ1_000367"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM9429341.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15908..16132
                     /locus_tag="ACHBJ1_000368"
     CDS             15908..16132
                     /locus_tag="ACHBJ1_000368"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM9429342.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16134..17024
                     /locus_tag="ACHBJ1_000369"
     CDS             16134..17024
                     /locus_tag="ACHBJ1_000369"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9429343.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17033..17851
                     /locus_tag="ACHBJ1_000370"
     CDS             17033..17851
                     /locus_tag="ACHBJ1_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM9429344.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17958..18407
                     /locus_tag="ACHBJ1_000371"
     CDS             17958..18407
                     /locus_tag="ACHBJ1_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9429345.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18425..20107
                     /gene="recN"
                     /locus_tag="ACHBJ1_000372"
     CDS             18425..20107
                     /gene="recN"
                     /locus_tag="ACHBJ1_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM9429346.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20162..20278)
                     /locus_tag="ACHBJ1_000373"
     CDS             complement(20162..20278)
                     /locus_tag="ACHBJ1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM9429347.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20351..21307)
                     /locus_tag="ACHBJ1_000374"
     CDS             complement(20351..21307)
                     /locus_tag="ACHBJ1_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9429348.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21478..21846
                     /locus_tag="ACHBJ1_000375"
     CDS             21478..21846
                     /locus_tag="ACHBJ1_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM9429349.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22016..22447
                     /gene="mraZ"
                     /locus_tag="ACHBJ1_000376"
     CDS             22016..22447
                     /gene="mraZ"
                     /locus_tag="ACHBJ1_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM9429350.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22468..23427
                     /gene="rsmH"
                     /locus_tag="ACHBJ1_000377"
     CDS             22468..23427
                     /gene="rsmH"
                     /locus_tag="ACHBJ1_000377"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM9429351.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23432..23833
                     /gene="ftsL"
                     /locus_tag="ACHBJ1_000378"
     CDS             23432..23833
                     /gene="ftsL"
                     /locus_tag="ACHBJ1_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM9429352.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23834..26026
                     /locus_tag="ACHBJ1_000379"
     CDS             23834..26026
                     /locus_tag="ACHBJ1_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9429353.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26052..27014
                     /gene="mraY"
                     /locus_tag="ACHBJ1_000380"
     CDS             26052..27014
                     /gene="mraY"
                     /locus_tag="ACHBJ1_000380"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM9429354.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27028..28389
                     /gene="murD"
                     /locus_tag="ACHBJ1_000381"
     CDS             27028..28389
                     /gene="murD"
                     /locus_tag="ACHBJ1_000381"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM9429355.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28415..29503
                     /gene="murG"
                     /locus_tag="ACHBJ1_000382"
     CDS             28415..29503
                     /gene="murG"
                     /locus_tag="ACHBJ1_000382"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9429356.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29518..30738
                     /locus_tag="ACHBJ1_000383"
     CDS             29518..30738
                     /locus_tag="ACHBJ1_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM9429357.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30894..32219
                     /gene="ftsA"
                     /locus_tag="ACHBJ1_000384"
     CDS             30894..32219
                     /gene="ftsA"
                     /locus_tag="ACHBJ1_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM9429358.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32242..33483
                     /gene="ftsZ"
                     /locus_tag="ACHBJ1_000385"
     CDS             32242..33483
                     /gene="ftsZ"
                     /locus_tag="ACHBJ1_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM9429359.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33500..34177
                     /locus_tag="ACHBJ1_000386"
     CDS             33500..34177
                     /locus_tag="ACHBJ1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM9429360.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34191..34790
                     /locus_tag="ACHBJ1_000387"
     CDS             34191..34790
                     /locus_tag="ACHBJ1_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM9429361.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34871..35092
                     /locus_tag="ACHBJ1_000388"
     CDS             34871..35092
                     /locus_tag="ACHBJ1_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM9429362.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35141..35923
                     /locus_tag="ACHBJ1_000389"
     CDS             35141..35923
                     /locus_tag="ACHBJ1_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM9429363.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35944..36651
                     /locus_tag="ACHBJ1_000390"
     CDS             35944..36651
                     /locus_tag="ACHBJ1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM9429364.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36913..39711
                     /gene="ileS"
                     /locus_tag="ACHBJ1_000391"
     CDS             36913..39711
                     /gene="ileS"
                     /locus_tag="ACHBJ1_000391"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM9429365.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39760..41286)
                     /gene="zwf"
                     /locus_tag="ACHBJ1_000392"
     CDS             complement(39760..41286)
                     /gene="zwf"
                     /locus_tag="ACHBJ1_000392"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM9429366.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41301..41948)
                     /locus_tag="ACHBJ1_000393"
     CDS             complement(41301..41948)
                     /locus_tag="ACHBJ1_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9429367.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42132..42461)
                     /locus_tag="ACHBJ1_000394"
     CDS             complement(42132..42461)
                     /locus_tag="ACHBJ1_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM9429368.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42638..43366
                     /gene="sfsA"
                     /locus_tag="ACHBJ1_000395"
     CDS             42638..43366
                     /gene="sfsA"
                     /locus_tag="ACHBJ1_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM9429369.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43382..44395
                     /locus_tag="ACHBJ1_000396"
     CDS             43382..44395
                     /locus_tag="ACHBJ1_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9429370.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44395..45672
                     /locus_tag="ACHBJ1_000397"
     CDS             44395..45672
                     /locus_tag="ACHBJ1_000397"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9429371.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45735..48437
                     /locus_tag="ACHBJ1_000398"
     CDS             45735..48437
                     /locus_tag="ACHBJ1_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9429372.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            48589..48906
                     /locus_tag="ACHBJ1_000399"
     CDS             48589..48906
                     /locus_tag="ACHBJ1_000399"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9429373.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48936..49256
                     /locus_tag="ACHBJ1_000400"
     CDS             48936..49256
                     /locus_tag="ACHBJ1_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9429374.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49364..50824
                     /locus_tag="ACHBJ1_000401"
     CDS             49364..50824
                     /locus_tag="ACHBJ1_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9429375.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50892..51113
                     /locus_tag="ACHBJ1_000402"
     CDS             50892..51113
                     /locus_tag="ACHBJ1_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429376.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51144..51326
                     /locus_tag="ACHBJ1_000403"
     CDS             51144..51326
                     /locus_tag="ACHBJ1_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM9429377.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51326..51739
                     /locus_tag="ACHBJ1_000404"
     CDS             51326..51739
                     /locus_tag="ACHBJ1_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM9429378.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51862..53043
                     /locus_tag="ACHBJ1_000405"
     CDS             51862..53043
                     /locus_tag="ACHBJ1_000405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429379.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53114..53278)
                     /locus_tag="ACHBJ1_000406"
     CDS             complement(53114..53278)
                     /locus_tag="ACHBJ1_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429380.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53432..53505
                     /locus_tag="ACHBJ1_000407"
     tRNA            53432..53505
                     /locus_tag="ACHBJ1_000407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53466..53468,aa:Arg,seq:cct)
     gene            complement(53574..>53873)
                     /locus_tag="ACHBJ1_000408"
     CDS             complement(53574..>53873)
                     /locus_tag="ACHBJ1_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429381.1"
                     /translation="FVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHAS
                     ILLYQGVNILSVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        17782 a         9140 c        11638 g        15313 t
ORIGIN      
        1 tattttagaa aatttcatga aaaaggagtt agataggtgt tcaaaaatta tgtttcctca
       61 ctaaaaaaag aattttcagg ttatgatctt tcaagatttc agaaagatct tttggctggg
      121 atcacagtag ctgctgtagc attgccttta gccttggctt ttggggtatc aagcggagct
      181 gatgcggccg caggtttgat tacggctatc attgccggta tcgttattgg cggtttatca
      241 ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg cagctatctt gatgtctatt
      301 gcggcaacaa agggaatgca aggtgtattg cttgctactt ttttagcggg tctattttta
      361 cttgcagcag gacttttccg attaggaaca ctgacttctt ttatcccagc acccgtaatt
      421 acggggttta cttcaggtat tgctatcatt attgcattag gtcagatcga taatttattt
      481 ggtgttcact cggaaggtac aaacgttatt gaaaaactaa gtagttatca aacgatagga
      541 tttccgataa acacagcctc tttgatcatg ggagggctag tcattttagg gatgtttatc
      601 tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc tggctatcat tttggcaaca
      661 gcagttatgc tattgttcca tttaccagta gcaactgtgg gtaagattcc acaaacattg
      721 atcagtagta atcgtttgaa tatgggagat tttagctttt ctgccttgca acaagtggca
      781 gttccagcta ttagcattgc tttattagga atgattgaaa gcttgctatg tggtgcttcg
      841 gctggccgaa tggcaaataa gcccttagat agcaatcaag aattaatggc gcaaggaatt
      901 ggaaatctgt tgcttccttt ctttggcggg attcctgcta ctgcggccat cgcccgtaca
      961 agcgtggcca tcaaatctgg tgcacaaact agattagctg gtatgataca tgcagttgtc
     1021 ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac agtttccaat gccagcactt
     1081 gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat gggaaacgat caaggagcta
     1141 tttactaata aatattggtc agcagttcta ttgtttatcc ttacgatgat ttgtacagtt
     1201 atctttgatt taagtattgc tatcgtgatc ggggtgatcg gcggctgtgt attttttgtt
     1261 gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg attggcaaag aatggggctg
     1321 ccagaaacgg aaaagctgaa taactggaca gtagtgtata ttagtggtcc actgtttttt
     1381 atgtcttcag aaaaactcaa aaataaattg gaagaactaa atcatcacga tggtgtcatt
     1441 ttctccatgc gaggcgtacc aagtattgat ttgacggcac gtgcagtttt tgatgaattt
     1501 caagaaaaag cagagcaaag aggacaaacg attatctata cttccttgca gccagaagta
     1561 gaaaaacaac tttctcactt gtgggaacat acattcacgc atcaacatca aacagttgct
     1621 catgcgttga cagctttaca tgcgaaatat actgcaatta gccattaaaa caatgaaaaa
     1681 gaacagacaa gactgtaaac tgaagatttt atggtcttgt ctgttctttt taatattgct
     1741 gtaaaagtga agctagtact tgccagtctt gatggataac aggtacaagg gagcgattgt
     1801 agaaaatagc tgctggatga aaaagtggaa gaattcgata gtttttttct gaccattcgt
     1861 aggcatcctt ttgttctgtt aattgtaaga caggccctgt atagagttga ccatgatttt
     1921 tggtaaccgt atattctttg ccaagtaaac gttgaagacc aatgtttcca agtgtcgcaa
     1981 ttaattttgg ctggaccttc atcagttcgt aatccaatat tggtgcatgc gcaaatactt
     2041 cggaacgagt aggtgtccga tttggataaa cagtttctga ctgttgtgtc cgtttattga
     2101 tccgatgagt cacgcgatac gggcggctcc ttacagcact ggtgatatac acctcttctc
     2161 gtgttaagcc agttgaagaa agtgcctgca tcaattcttt tccagcttga ccgctaaaag
     2221 gaacatgcgt atcaatctct gttttccctg gggcttctcc cacgagcatc agtttaggat
     2281 gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg gccgttcatt cttaaagaag
     2341 cttcttgtat cagttctttt gggtattcca tttatgctcc tcctttttct tttattataa
     2401 gaataaaaga aacacaatgc gactatttta tttggtagaa ctgtgaggat agaaaagcag
     2461 aagtattgct tgaatttttg ttttactttt tgattttaaa tttagttgac taacggttgc
     2521 tgttctggta agctagtact gttgtaattg tggactccac agctacaacc gctcagaaca
     2581 agtaataagc acagcatatg ctgtcacttc ggatggcgag tctaagtata tcagaaggag
     2641 gtgctttata gcatgtacgc aattattaaa actggtggta aacaagtaaa agttgaagtg
     2701 ggacaagcaa tctatgttga aaaacttgac gttgaagcag gagaaaaagt tgtttttgac
     2761 gaagttatct tagtaggcgg agaatctaca aaagtaggcg ctccaactgt tgcaggtgca
     2821 actgttgaag gtactgttga aaaacacggc aaacaaaaga aagtcgttac tttcaaatac
     2881 aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc aaccatatac aaaagtggtt
     2941 atcgacgcga tcaacgctta atagcgtttg agaaagaagg cctatatatg atcaaaggaa
     3001 catttaagcg aaatgacgca ggtcagatcg tttcattcac tttgaccggg catgctgatg
     3061 ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc tgcgttagcg atcagcactg
     3121 tcaacgggat tgcctcatta gccggttttg agccgattgt tgaaatgaat gaagaagaag
     3181 gcggttatct ttataccgaa gtcacttcag gaatgacaca agaacaaaac aatattgctg
     3241 agattctatt agaaaatctt ttattaggat tacaatcgat agaagctgaa aactcagaat
     3301 atatccaaat taaaacaatt aatgataaat aggaggtgca gaccatgttg ttatctatga
     3361 atctacaatt atttgcccac aaaaaaggtg gcggttctac atctaacggc cgtgactcag
     3421 aatcaaaacg tctaggtgct aaacgtgctg atggacaaac agttacaggt ggatcaattc
     3481 tttaccgtca acgcggtaca aaaatctatc caggtgtaaa cgtgggtatc ggtggagacg
     3541 acactttatt tgcaaaagtt gatggcgtag tacgtttcga acgtaaaggc cgtaacaaaa
     3601 aacaagtgtc tgtttatcca gtagctcaag aagcttaatt gaatcttatg attaaagagg
     3661 ttgtgacaag acttttgtca cagccttttt tgtttataaa aataaaagtt gtagtggagg
     3721 ctacgaagtt tactttgtct ttttacactt aatatttttt tgccaatttg gaatcaggat
     3781 gttttgataa ctatcgtttt caattgatgg aagctatatt gtttgctatc agtgaaataa
     3841 ttatatggta aacagtgttc tttttcttgt ttccattata aaaatcgtat cgggattttt
     3901 cgttctttta aattgaacat ttctatctat ccgcgtgtta tcaacaaaac aaaatcaggt
     3961 aaatagttga cgaaacagaa ataaatacat agtattatgt tttgaagcct aagtacaata
     4021 gtctatagaa aaggagcgat accattggca aaacgcattg accgaattta tatgtatgtc
     4081 aaagaaaaaa ccgctcattt aacaaaggca gaatatgatc aaggagtaac aacacaggaa
     4141 attgctgaag tgttagggat tcagcgaaca aatagcagta aagatctgaa tcagctagtg
     4201 cgcgaaggaa aactgataaa gacagacgga cgaccagtac gttacatata tcaaacactc
     4261 gttacgcacg taaagccagt tacaaaacat gtagtcagtt acaaagaaac gccggtctct
     4321 gaagaaaaaa atacactcca aatagatact aaggatatct ttgcaaaaat cattggagct
     4381 aacggcagca tgaaaaattc agtggaacag gcaaaagctg cgattttgta tccgccaaga
     4441 ggattaaatt gtttgattac aggacctacc ggatcaggaa agacttattt tgcacacgcg
     4501 atgtatcatt ttgcaaaagc gcatcatgta atagatgagg aaacggaact gattgttttc
     4561 aattgtgctg attatgccaa taaccctgag cttttgatga gtcatttatt tgggtacgca
     4621 aaaggtgctt ttacaggcgc agatgaagaa aaaatgggga ttatcgatca agcggatggc
     4681 ggtatgctgt ttttggatga gattcaccgg ttgccgcctg aaggtcaaga aatgatcttt
     4741 tactttatgg atcatggaac ttacagtcgt cttggggaaa caacgaaatc ccatgaagca
     4801 aatgtacgga tcgtaggtgc gaccacagaa gacccaggtt cttctctatt agaaactttt
     4861 gttcggcgta tcccaatcaa tatcaaactt ccggcttttg aaaaacgtcc tgcaaatgag
     4921 aaagtcgatt tagtaaaaat catgattgcc cacgaagcaa accgcacgca gcgaaagatt
     4981 tctttgacag aagatgtagc aaaagcattg atcggcagtg tgacctatgg aaatatagga
     5041 caattgaagt caaatatcca gttagtatgt gcacgaggat ttctaaatca tatgcaatca
     5101 cccgaaatct cgatcacaat cgatgatttg acagaaggga ttcgtagcgg attgattcaa
     5161 ttagcaagca accgcagtgc aatggcagag ctttctaaaa tattggagcc aaagataact
     5221 gtagcaccaa atgatacgat catgaaaatc caatcggact cctatgagtt accttataat
     5281 ctatatgata tcatcggaga taaagcagct ttactgaagt cggatggttt ggatcaagaa
     5341 gcaatcaatc atttcatttc tacagatatc aacatccatc tgaagtcttt ctataaagac
     5401 catggttttt cttttaatgc agacagcaaa ttagcagaat ttgtagatcc gaaaatcata
     5461 gaagtgacca atcagattta tgtaatggtc aaaagatctt taccatatga gtttcagcaa
     5521 aactttattt atgctatgag cttgcatatc agttcttttt tgaaaaaaat caacttagga
     5581 gaagaacggc atacgaatga taacatccgt gagatggctt ttgattttcc agatgaatat
     5641 gctgttgcga aagaagtgcg tagttatatc gaaacgtatt ttcaagtgga gattccagaa
     5701 agtgaagact attacttgac cgttctactc gtctcgttac gggcaaatca agcaagcggc
     5761 agaatcggtg tagtcgttgc agctcacgga aacagcacag caagcagtat ggtccaagtt
     5821 gtccaacagt tgttagatgc agagaacgta cgagcagtag acatgccttt ggatatggat
     5881 ccgaaaacag cattggaccg aattgaacgc aatgtacaag aagtagatga aggaagcggg
     5941 gtcatactct tagtagatat ggggtccctt gcgtcgttta attcgcaaat tcaaagagac
     6001 actggaattc cagtacgtac tgttgatatg gtaacaactt ctctagtttt ggaaactgta
     6061 cgaaaggtct cagtattagg gacagatcta gatatgttgc atgaatcatt gaaaaatttt
     6121 cgaggttacg cagaaatatc ttctgtagaa tctgttcagg aagcaaaacc tattcataag
     6181 aaaaaagctg ttttggctat ctgcgcatct ggcgaaggta cagcacaaag aatcaaagaa
     6241 ttgatcgaac gagcggtaaa caagcgtcaa gaaacagaat tagaagttct agctttgtcg
     6301 attgtggaag tgaaagaaga actgccagtt atccaaaaag aatatcagat catagctact
     6361 actgggatta ctgatttgaa tatccaagcc ccatttattc ctttagaacg ttttattgat
     6421 caaaatatcg aagtcatttt ggatcagttg ctgatggaat cagaattgga ggaaactgaa
     6481 ttcatctccc tagatgaaga gtcagctaaa aatacgtgtg ttgagtttat ctctgataac
     6541 ttcatcttta ttaatggtag taagttaatc gatccaatgt ggcaattttc tacacaaatc
     6601 tctcagacga cgggaatcgg cgatgaagaa tatggattca aaatcaacct agtgatgcac
     6661 acagcaggaa tgatcgaacg tattattcgg aatgaaccat tgactgtgga agaaaacgaa
     6721 ttgacaaata caacgaatga tcctctttat agccaattgg cagcctcagt tgttctgttg
     6781 gaagatcaga taaaagtaaa agtgccgata gaagaaatgt attatctgct acgtctagtc
     6841 cataatcagt tagataaaaa agaatacact gtgccatgaa tacacaaaaa cgatacactt
     6901 ttatagtgta tcgtttttgt gtattggtta agaatccttt aaattcaatt agtgtatcgt
     6961 aaaaaagttg gcacgctttt tgcttatagt agtgtgtgct aggaggtggt tgttcacaca
     7021 gaaatgatag gggtttctgt gaagtagtga ggaagaagga gttttgcaga tggatattcg
     7081 attggcacgt atagatgatc gtttaatcca cgggcaagtc gcaacagttt ggtcgaagca
     7141 aacaggtgtt gaacgtatca ttgtgatcag cgatgtagtc gctcaagata aattgagaaa
     7201 gtttctacta aaagaagctg caccgccggg gatcacagca aatgtaataa ccgtgcagaa
     7261 aatgattcaa atctatcata gtggattatt atcacgagca aaagtgatgc ttttattcac
     7321 caatccccaa gatgttgaag cggttgtccg ctgcggtgta cagttgaagt cgttgaatat
     7381 cggcggtatg agttttactg aaggcaaatc aatgattacc aattttgttt cagtaaatca
     7441 acaagatatt gattcctttc gttacttgaa cagtcaagaa gtggagttag agattcgaaa
     7501 agtacctgct gacaggaaaa tccttttgat ggaactacta gcgaaaatca aaaaggtgta
     7561 gcaagaatgg ttgttggaaa aggtcaaata attctgttgt aatggttagc aattattaat
     7621 atttttgaat ttgttacaac aaaagtaaat aggaggtata aatatggtag ggattatcct
     7681 agcaagtcat ggacaatttg ctgaaggaat cttacaatcc ggttcaatga tctttggcga
     7741 acaagaaaac gtcaaagccg tgattttgaa accaagtgaa ggtccagatg acttgagagc
     7801 caaattggaa gaagcggttg cttcttttga taatcaagac gaagtgttat tcttagttga
     7861 tttatggggc ggaacaccgt tcaatcaatc aaacacatta ttcgaagagc acaaagataa
     7921 atgggcaatc gtcagcggtt tgaatttacc aatgttgatt gaagcctacg catcacgctt
     7981 ttcaatggaa tctgctcatg aaattgcagc acacatcatt gaaacagcaa aagacggtgt
     8041 aaaagtaaaa ccggaagaat tagaaccggc agaagcaccg aaagcagcag ttgaagacgc
     8101 acaacctaaa ggagcacttc cagaaggaac tgtggtcgga gacggaaaaa tcaaatatgt
     8161 attagcacgt gttgactcac gtctgttaca tggacaagtt gcgacagctt ggacaaaagc
     8221 agtgcaacca aaccgtatta tcgttgtttc agatgcagta tcaaaggatg atctgcgtaa
     8281 gagactaatc gaacaagctg ctcctccagg tgtaaaagca aatgttattc ctattagcaa
     8341 aatgatcgaa gtagcaaaag accctcgttt tggaaatacc aaagcattgc ttttatttga
     8401 aaatcctgaa gacgtattga cagctgttga aggtggcgta gacatcaaag aattaaatgt
     8461 tgggtcaatg gcgcattcgg tcggtaaagt cgttgtaagt aaagtattgt ctatgggaca
     8521 agaagacgtt gaagcgtttg agaagcttga acaaaaaggt gtgaaattcg atgtacgtaa
     8581 agtacctaat gattcacgcg ataacatgga tgatattttg aaaaaagcaa aagctgaatt
     8641 agcaaaagcg taataaataa tattaaaatt tataggaggc ttatcatgtc tattatttca
     8701 ataattttag tcgtattagt tgcctttcta gctggtatgg aaggtattct tgatgaattt
     8761 caatttcatc aaccactagt ggcatgtaca ttgatcggtt tagttaccgg taacctcgaa
     8821 gctggtatcg tacttggcgg tacgcttcag atgatcgctt taggttgggc aaatatcggt
     8881 gccgctgtag caccagatgc tgcattagca tcagtagcat cagcaattat tttagtttta
     8941 ggtggacaag gtgttaaagg tgttccttct gcgatcgcga ttgccgttcc tttggctgtt
     9001 gcagggttgt tcttaacaat gatcgtacgt acaatcgctg ttccaatcgt tcatttgatg
     9061 gatgcggctg ctgaagaagg aaacatccgt aaggttgaaa tgtggcacat cattgctgta
     9121 tgtttacaag gggttcgtat cgcggtccca gcagcagcgt tgctatttat cccagcacaa
     9181 actgtgcaat cattccttga atcaatgcca gcatggttaa cagatggtat ggctatcggt
     9241 gggggaatgg tcgtagctgt aggttacgct ttggtaatca acatgatggc tacaaaagaa
     9301 gtatggccat tcttcgttat tggtttcgtt gtagctgcaa tttctcaatt aacattgatt
     9361 gctctaggtg ctttaggtgt tgcattagct cttatttact tgaacctttc taaaatgggt
     9421 ggttcatcaa ataatggagg cggaagcaac tctggtgacc ctttaggcga cattttgaat
     9481 gactattaat cttgaaggag gaaaataaaa tggcagaaga aaaaatcaaa ttatcaaaaa
     9541 aagatcgttt agctgttgcg tggcgctcta ccttcattca aggttcatgg aactacgaac
     9601 gtatgcaaaa cggtggctgg gcattttcta tgattccagc aatcaaaaaa ttatacaaaa
     9661 caaaagaaga acgttctgca gcattaaaac gtcacttgga attctttaat acacacccat
     9721 acattgcttc tccaatttta ggggttacat tggctctgga agaagaacgt gcgaatggcg
     9781 cgcctgttga tgatgtagcg attcaagggg ttaaagtagg tatgatgggt cctttagctg
     9841 gtgttggtga cccagtgttc tggtttacag tacgtccaat gctaggtgca ctaggtgctt
     9901 cattagctat gggaggaaat atccttggac caatcatctt cttcttaggt tggaacatta
     9961 ttcgttgggc attcatgtgg tatacacaag aatttggcta caaagctggt tctaaaatta
    10021 ctgatgacct ttctggcgga ttgttacaag acgtaacaaa aggtgcttca attttaggga
    10081 tgttcgtatt agctgccctt gtacaaagat gggtatcaat caaattctta ccagttgtat
    10141 caactgtaaa attagataaa ggtgcttaca tcgaatggga taaattgcct tcaggtggtg
    10201 aaggaatcaa gagtgcattt gaacaagtta ataatggctt agccctttct cctgaaaagg
    10261 taacaacatt acaagacaac ttagaccaat tgattcctgg tttagctgca ttaggattaa
    10321 cattgttatg tatgtggttg cttaagaaaa aggtttctcc aatcgttatc atccttggtt
    10381 tattcgtagt cggtgttgtt ggacacgtca tcggactttt ataatccata aaaatatagc
    10441 tcgggcgttg tcccgagctt tttttatata gtatactgtt cataaacaaa tatctggagg
    10501 gagaaaatgg ttcaatcaat caatacaaaa gtagacttgg taattgatgc tacttcgttt
    10561 acgggtctga cagattacgg gcaaatcatg ataggtaata atggttttga gttttaccat
    10621 gcgcgagata gacgcaaata tatccaaatt ccatgggaag aagtggatta tgtactagca
    10681 tcagtcatgc taaagggaaa atggattcct cgatatgcca ttcaaacgaa aaaaaatggt
    10741 acatacacat tttcttccaa agaacctaaa aaagtgcttc gtgcgatcag agaatatgta
    10801 gatgaagata gaatggtaca atcattgagt ttctttgatg tagtgaaacg tggtattcgt
    10861 agtatgttta aacgcaagta aaaaataaag aaaacttcta agaggaggtt ttctttattt
    10921 ttttactttg cagaagaaaa tatgaaatag caaaaccaat tatttgtccc ctttaaatcc
    10981 catatgctaa aattgattag caaagaagga ggttatatta tgagttggtt atggacattg
    11041 attgttggag ccgtgatcgg agcaattgca ggtgcgatca ccagtaaagg aaattcgatg
    11101 ggctgtgtaa cgaatatcat tgctggacta gtcggttcaa gtattggaca agcgattttc
    11161 ggcgaatggg gaccaaaagc tgcaggtatg gcacttattc catctatttt aggcgcagtg
    11221 attttggtag cagttgtctc atttttcttt ggaaaacgat cataagtttt tacaaaatag
    11281 taaactaaaa ggagttgcag cgagactgtg actccttttt ggctttattt atttaaaata
    11341 aaaagaagtt ttccggaaat ttttatttaa ctgtcgtgta cgaagaaaag tttgctataa
    11401 tggaaaaaag cgtgcaaaga aagagggagt ttcattatga tgctacgtgt agaaaaattg
    11461 cgcaaaaaaa tgcaagaaga aaatttggat tcatttttgg ttaccagtcc gtacaatctt
    11521 cgttacttaa cgaactttac aggtacgaca ggattagctg ttattacttt ggaaaaagca
    11581 ttttttatta ctgattttcg ttataccgag caggcggcag cgcaggctca agggtttgaa
    11641 attattaaaa atgtcggacc tatttttgat gaggtagcaa acttagtacg caaagaagaa
    11701 ctgcactcat tagcttttga ggaaacaaca gtctcatttt tagaatattc ggttttagaa
    11761 gaaatcatcg atgcggaatt agtacctgtt tcacagatga tcgaagaact cagagaagtg
    11821 aaagatgaag aagaaatcgc tattatcgaa aaagcatgtc atattgcgga tatggcatat
    11881 gatcacatcc tgaaaatgat ccaaccgggc atgactgaga ttgaagtcgc aaatcaacta
    11941 gacttttata tgcgttctct tggcgcttca ggtgtttctt ttgatacgat cgttgctagc
    12001 ggattgcgtt cggctatgcc gcatggtgta gcaagcaaaa aaatcatcga acaaggtgac
    12061 ttgatcacat tggactttgg ttgctattac gaagggtacg tttccgatat gacacgtaca
    12121 ttcgctattg gtgatcctgg ggaaaaatta aaagaaattt atcaaatagt cttagatgct
    12181 caattagcag ttattgatgc tgcaaaacca ggaatgactg gtgtccaatt agatgcagtt
    12241 gctagagatt atatttcaaa acatggttac ggggaagcat ttggacattc tacaggacat
    12301 gggatcggtt tagagatcca cgaaggacca aacgtttctt ctcgtgcaga aaagcaattt
    12361 gtaccaggaa atgtgatcac tgatgaacca gggatctact taccaggaat cggtggagta
    12421 cgtattgagg atgacttgtt gatcacagaa gatggaaatc gtgtgttgac tcgttcacca
    12481 aaagaattga tcatcttata gtttcatcaa tagaagaagt aagcgtaaaa atcagtgttc
    12541 acagactttg ttatagaaaa tcactcgatt taatgataca ctatagaaga acttttaagc
    12601 tgtaagagat aataaagttg ggtgttttct ccaattcata gcagtttgca gctgatcggc
    12661 ggcagttttc ttaccacgtg atcactatgc cgcatcatag agaattcgga tgtatctcgt
    12721 gcttaggcaa agggaggaat aaataaatgg cagatgaaaa aaatttgatt ttaaacgcaa
    12781 accaagattt aggagagatc gtgatcgctc ctgaagtcat cgaagtaatt atcgggattg
    12841 cagcttctaa agtagaaggc gtatacggaa tgcgcggaac ttttgccagc aatgtcactg
    12901 aattattagg acgtgcagct cacggaaaag gtgtttactt agcaaacgag gaagatggct
    12961 tgaaagtaga tctttactgt tatctagaat atggtgtttc agttcctaaa gtagcaatgg
    13021 aaatgcagga acgggtcagc cagcaagtcc ttttcatgac ggatatcgat ttatctgaag
    13081 tcaatatcca tgtagtagct gttgttcctg aaaaattaga acaaccaaca atcgaagaac
    13141 tttttgaaga cgaagaggaa gagaatgaat aaagaattat caagacacga gattcgggaa
    13201 atggcattgc aggctttatt tccattagac ttcaatgcag acctaacaaa agaagatgcg
    13261 attttcaacg caatcgaatt agaccaccgc gacatgatca acgaagatga atcagagttt
    13321 gttcctgttt atcttgatac gctagttggc ggtgtctgtg caaaaaaaga tgaattggat
    13381 aaagtgattg aaaaacattt aaaaaacaat tggcacatca atcggatctc aaaaatggat
    13441 ttggtgatct tgcgtatcgc gatttttgaa atgacttatg tgacagatgt accagcggct
    13501 gttgctttaa atgaagcaat cgagttggca aaaacattta gtgatgaccg ttcacgtaaa
    13561 ttcgtcaacg gtgttttatc caatgtattg aaagaattag aagctggggc gtaagtccta
    13621 gcttttttct atgaatcatc ataaacctgt tataaatcgg gaaaattcta tggtagaatg
    13681 aaaacacaaa aaatatctgg gagtggttag atggcagaat tgattaacgg aaaagaatta
    13741 gctgaaaaaa tgcaggcaga gattgctgaa aaaatccaaa aattgaaaga taatggtatc
    13801 catcccggat tagtcgtttt attggtaggg gaaaatccag ccagccaaat ctatgtaaga
    13861 aataaagaac gtgtagcaaa agaaatcgga atccattcat tggtagagcg ttatcctgag
    13921 acgatttctg aagacgaact acttgcagaa attgaaaaat acaatcaaga tcctagtttt
    13981 catggtatat tggtccaatt accattgcca aagcatatag atgaagaaaa gatcctgtta
    14041 gctatccgcc ctgaaaaaga cgtagacggt tttcatccga tgaatttagg cagactcctt
    14101 gcgggtaaac cggacaagat tccttgtact ccatacggca tcatgaaaat gtttgaagct
    14161 tattctatcg atccggcggg taaacgagca gtagttatcg gacgaagcaa tatcgtcggt
    14221 aaaccgatgg ctcagctact cttgatggca gatgcaactg ttactgttgc tcattctaaa
    14281 accgaaaatc tggctgaact ggcacaagaa gcagatattt tagtcgtagc aataggacgt
    14341 ggacattttg tcacaaaaga attcgtaaaa ccaggtgctg tagtgataga tgtagggatg
    14401 aatcgtgatg ctaacggtaa gctgatcggt gatgtgaaat ttgatgaagt ggagcctatt
    14461 gcaggctgga tcactcctgt gccaaagggt gtagggccaa tgactatcac gatgctgatg
    14521 tatcaaaccg taaaatctgc cgaagctttt ggagcaggtg aatagatggc acaagaatat
    14581 ttaactgtca ctacattaac gaagtatttg aaaagaaaat ttgatgccga tccttattta
    14641 gaacgcgtat atttaacagg cgaaatatcg aatttccgtt tacgtgccaa tgcccatcaa
    14701 tatttcagct taaaagatga ccatgcaaaa atatctgcta ttatgttcaa aggagcattc
    14761 caaaaattac gattccagcc aaaagaagga atgaaagtcc ttgtcgttgg gcgtatttca
    14821 ttatatgaag cgggaggttc ttatcaaatc tatgtagagc acatggagcc tgatggaata
    14881 ggtgctttat atcaagaatt ggaagaacga aaagaaaagc tgatgaagga aggccttttt
    14941 caagggccta aaaaaatgct gccacgtttc ccaaagcgaa tcgcggtcct taccagtcca
    15001 agcggagcgg ttatccgcga tatcatcacg acggtgaaaa gaagatatcc aattgtccaa
    15061 ttggttttgt tcccaacagt tgtacaagga aaccaagcag cggacaatgt tgtcaggaat
    15121 atccagagag tagaagagat gggcaatttc gatacaatga tcatcggtcg tgggggtggt
    15181 tcgattgagg atttatggcc gtttaatgaa gagcgggttg ctcgggcgat ttatcaagct
    15241 aatacgccgg tgatttcatc tgtaggacat gaaacagata ccacgatcgc tgatttagta
    15301 gcagatgttc gggctgccac tccaacagca gctgcagaat tagctgttcc tgttttgtca
    15361 gaagagttga tgcgcattca agaacggcaa gctagattgc agcaggcgtt tgtacgccag
    15421 atccagcgca aacaagaacg gttcgagcgt gctcgacgtt cctatgtatt tcggcagcct
    15481 gaaagattgt atgaaggtca gaccatcaaa ttagaccagt tgaaacagcg attatatcaa
    15541 gctacacagc aaatttatca tgcgaaagaa aaacaaacga tcgcattaag ccaccaattg
    15601 gaacaagtgg ctccgcttta tcgagttaaa gcagctcgcc aagaaactgc ttatctagaa
    15661 aaacgcttag aagagaaaat ccaacaatat atgaaacaac agcagcagcg ttttgctcaa
    15721 gctgttcaat ctcttgattt gttgagtcca ttgaaaatca tgggaagagg ctacagctat
    15781 acgacgttaa atgatcaagt agtaaaaagt gtcaccgaga ttcatcaagg ggatttgctg
    15841 acgatccatt atcaagatgg aatcgttaaa ggacaagtac aagagataga caaaggagaa
    15901 taaaaatatg gaaaatccaa cttttgaaga gtcactgcaa gaattagaaa aaattgtcat
    15961 gcagttagaa caaggagacg ttccattaga atcagctcta gattcgttta aacgaggaat
    16021 ggaattaagc aaacattgcc aagatacatt agctaaagca gaagagacct tgacgaaaat
    16081 gatgacagaa tcaaatgaag aagttgattt tgacggaaac gaggaaagct gagatgagat
    16141 taactgactt ttcagctatt catctacctg ctgtagaaaa agaaatcttg tcttttttag
    16201 atgagcatac aacggataga ggactctatg atgcgatgag ttattcagtg aaagcaggcg
    16261 ggaaaagaat acgtccctta ttacttttaa cggccgttgc ttcctttgat gagcctattg
    16321 atgttcccgt ctatcaagta gcagctgctt tagaaatggt gcatacgtat tccttgatcc
    16381 acgatgattt gcctgcgatg gacaatgatg atcttcgtag aggaaaacct accaatcata
    16441 aagtattcgg cgaagcatta gctatattgg ccggagatgg tttgcttaca ggagcattcc
    16501 agctaatcag tatggcacac ttaggtaatt ctcctaagct tctgctgctt cagcaattag
    16561 ctgtttgtgc gggatcacaa ggtatggttg ctgggcaagc tgcagatatc gagggcgaat
    16621 cgaaaaagct ttctttagaa gaattagcat ttattcatga aagaaagaca gggcatttga
    16681 ttcgttatgc cttgctggct ggtggcatat tagcgaagca acctgaagaa atcttattgc
    16741 tacttcaacg attagccgaa catttaggtt tagcttttca gataagagat gatctactcg
    16801 atgtgattgg tacaacgaaa acattaggga aaacagctgg taaagatgaa cgtatggaaa
    16861 aaaatactta cccgcgttta ttaggcctag aaaagacacg tgaggcgttg gagattgagc
    16921 tgttatccgc caacaagatc atagacaaat tagaagagga cgttttttct tttgatggcg
    16981 ggctgatgcg ccagatgatc aagcaatttg atgtggagaa atgagggaaa gaatggaaaa
    17041 agaacgcgta gatgtattag cggtcaaaca aggattattt gaaacaagag aacaagccaa
    17101 acgttcagtg atggctggtt tgatttacaa cgaaaaaaac gagcggtttg ataaaccggg
    17161 agaaaaaatt ccagtgagta gtgaattaag agtcaaaggt aaaaaacttc cttatgtttc
    17221 tcgaggggga ttaaaattag aaaaagcact caaagcattt gatctatctg ttgaaggaaa
    17281 aacattgtta gatattggtg catccactgg cggatttact gatgcagctt tgcaaaatgg
    17341 cgcaaaaatg agttatgctt tagacgttgg ctacaatcag ctagcttgga aaataagaca
    17401 agatcctaga gtcgtcgtaa tggaaagagt gaattttcgt tatgcaaaac ccgaagactt
    17461 taccttagga attccagaag ttgctgttat cgatgtttct tttatttcac taaaactgat
    17521 gctgcctcca ttacatgcta ttttaaaaga agagggagaa gtgattgctt taatcaaacc
    17581 ccaattcgaa gcaggcagag aagctgtagg gaaaaacgga attgttcgtg accctcagac
    17641 ccatcaaaaa gttttagaag acattttgtc atttgcggca caaggtggat atgatgtgct
    17701 ggaattaagt tattctccga tcacgggggg agaaggaaat atcgagttct tggctcactt
    17761 gaggaaagta cccgaaagcg gaacaatcaa ttcagctatt aatatggcag aagtggtttc
    17821 aaatgcacac gaacagtttg accataaata aattgtaact gtgaaaatca agtgagaagt
    17881 atgcggattc ttctttatta taccaaggaa ataagctatc attgattaag aagaatatgc
    17941 taaggagcga agacgtaatg agaaaaaaag atcggcatcg tttgattact cgattgttga
    18001 gtgagcaaga tatccgaaag caggaagaat ttgttgaaat cctgaaaagc aaaggaatat
    18061 ctgttactca agctacgatt tccagagata taaaggaact gaaactgatc aaggtaccgg
    18121 cttcagatgg cggctaccgt tatagcatac cagctgaaac aaacgaagat gtaaatacta
    18181 aattggaaaa actattaaaa gacgctttcg tggcagttga ccaaatggaa aaatttgtta
    18241 ttttgaaaac attgccggga aatgcttcgg cagcagcaaa tttgattgat aaacgttaca
    18301 agaaagaatt attttcgatt atcaatgatg acgataatgt tttgatgatt acacgaactg
    18361 aagaccaggc agttttattg aagaaagact tcctgcatta cctttaagtt gaggagcgaa
    18421 tgtgatgcta caagaaatca gtattactaa ctttgcgatt attccggaat tgcggctttc
    18481 ttttcacgaa ggaatgaccg cgctgaccgg agagactggt gcgggtaaat ccattattat
    18541 cgatgcttta ggtctgttag caggaggaag aggttcaagc gattatatac gtcaaggagc
    18601 agaaaaatgt gtgcttgaag ggttgtttga actaccaaaa caagaaggat tctctgaact
    18661 gatggttgaa ttagggatcg aaacagatga ggacaatctg attgttcgac gggatatgtc
    18721 cttaactgga aaaaatgttt gtcgagtgaa tggtcatatt atcacattag ctaatctgag
    18781 aagaatcggc agttatttag tagatatcca aggacaaaac gaacatcagg aactgctcca
    18841 gcctgaatca catcttgctt tattggatcg ttttggagat gtagctttcc aacagaagaa
    18901 aaagagttat cagcaagaat atatctctta tcgtgagctg gaaaaaaggg tacgaaaagt
    18961 gcagcaaaat gaaaaatcct atgttcagag aatagatatg ctccatttcc agcaagaaga
    19021 aatagctgct gctgatttgc aagtcggaga agaagagaaa ttaaaagaag aaagagaaaa
    19081 acttagcaat tatcaaaaaa ttgctgatgg attagctgca ggttacggag cactgacgaa
    19141 taatgaacaa aacagtgtag atggtgttgg cttggccgtt tcagaactgc aagggatcgc
    19201 ccatctagat gtggagtatg aagcaattta tgaaaatatc caaagtgctt actatttgct
    19261 gcaagatgca atcggcgata tgagtcgtca gattgattta ttggagttag atgagagtcg
    19321 tttagaagaa gttacgcaac gtttagaatt aatccgacag ttgaaacgta aatacggcga
    19381 atccattgag tcaatattgg cttactatga cgaaatcacc gaagagctag cttcttctga
    19441 tttttcagaa ggtcaattgg acaaaatgaa agaggaacta gagcaaaaag agcttctact
    19501 tcagcaacaa gccgcagatc ttcatgaagc gcgtaaaaaa atcgcaaaag aattggaaaa
    19561 atccattctg catgaattga aaagtctgta tatggaaaac actgagtttg aggtacgttt
    19621 cttaaaagaa gaaaatagac aattgaatca tgatggattt gaccaaatcg aattttacat
    19681 tacgaccaat ccaggagagc cactgaagcc tttggtgaaa gtcgcttctg gtggcgaact
    19741 gtctcggatg ttgcttgctt tgaaaactat tttttcttct gagcaaggtg tgacaagtat
    19801 tatttttgat gaagtggata caggtgtgag cggaagagtt gcacaggcca tcgcagataa
    19861 aatttctcaa atatcgaaat actctcaagt tttgtgcatc actcatctgc ctcaagttgc
    19921 tgcagtcgcc gactatcaat attatattgt gaaagcagtg attgatggac gtacgcaaac
    19981 ttctgtttca gagttgaaaa caaaagaaag agaagaagaa attgcacgta tgctggctgg
    20041 aagtgagatt acaaaactca cactcgaaca tgcaaaagaa ctattgaaac tcgcaaaaag
    20101 gacctgagcc acctcgcagt ggttcaggtc cttttcagca gctgatagct atattttatt
    20161 tttacatgac actcattaca gcagtcaata caacagatcc aatcgttaaa atcagcattg
    20221 tccaaatgac aaattttgtt actttagcaa aagtacttgt ttgttttttt tcacgcattg
    20281 ttttcaacct cgtcattaat tagttgattc aagtgtacaa tcttttttcc atttccacaa
    20341 gagggctttt ttataaaaac ttcccttttt ttaaataata ggtagttacg atacaaataa
    20401 ataatgttaa tagaactagc catataaagg catcatccct ttcagcaata ggtaacttaa
    20461 tgttcattcc atagatactg ccgacgattg tcggtatagt taaaataatc gttaaagaag
    20521 ttaaaatttt cataatatta ttcagattat tcgatacgat tgctgaaaaa gtctgattca
    20581 tttgttctac tagttttaat tgaatgcgag aagtagtcat tgcctgctct gtttccacca
    20641 atacatcacg taaatgattt ttcaaagcat gattttcttg gaaaagttta ttttgcgcta
    20701 gttctttcaa tgcatctaaa ttagcagaag tcgcttctct gaaataaacg aggctttttt
    20761 ggatatccat gatttgataa agttgtttgt tttcagtgga tacttgaagt ttggcttcca
    20821 aagtatcgca ttgagattga agcgtagcta aataactgtt gtaagagagt gtgacctgcc
    20881 aaatgaattg aaagaagata ttgagattag gcgaaatatc actttttgga aaggtctggc
    20941 taaagacttt ctttaaaaac agtggttcat gattcgtaat tgtgataagc ttctttttgg
    21001 gcgtcaaaat aatagagagc gggtatgtat ccacttgaaa ataaccgcta ggacttgttt
    21061 tagcaaaagg atattgtaac aataataacg ctggctttgt caactttgat tgattgaatt
    21121 cttctgtacg ggaattttct gcatcgtcca atatggaagt gatgtaatct gacggaattt
    21181 caaattgatg tgtcagctga tgaatctctt cttgattagg acgttctaca caataccaga
    21241 tcgtttggtc agtttccttt ccggtattac gcagtttctc attatctaat gtaaaatatt
    21301 tgatcatgat tttcctcctg ttgcctgttt cttttattat aacggatagg gaattggaat
    21361 acatggaatg tgtttgtaag atatagaata taactgcata aatttaaata aaatgaaaca
    21421 gttgtgtaaa gtatatacag tatacttgaa gcaacgataa aaaaggagtt tacgttcatg
    21481 ggcacattca caccacttat cttgttttgg tatatttacc cggtaatcgt tttgtttggt
    21541 tgccaatttc ttgtaaaatt attttcgtta aatcgtcgtt ttaaaataaa agcccctgat
    21601 ttggcgattc cttttttgtg gggcggtatg catgcattgt ctagaaatac agggacaatt
    21661 tctatcttac cgtttttctt tatttctgtt ttgctaatgg gaatttgtat tgctgttttc
    21721 caagcatatt attatgaaga aattatttat ccgcgttatt tcaagatgac ttggcgtttg
    21781 gtttttctgt tgacaatcgt cttgtacgct gtcttgatca ttgtgaacat actttcttac
    21841 ctatgaaaaa aatagaaaag ctgtaaaaag tcttttaaaa ggctttttgc agcttgattt
    21901 ttaaaaaaat gtttatgaat aaaaaaagtt gttcccggat ttcccagaaa aggtggtaga
    21961 aagtggggga ttgtggtaga ctaattttag ttagtggagg aatggggggc tttcaatgtt
    22021 gatgggtgaa ttccaacata atattgatgc aaaaggacgt ttaatcgtgc cctcaaaact
    22081 tcgtgaagaa cttggcgaaa agtttgttct gacacgcgga ttggatggct gtcttttcgg
    22141 ctatccgatg agcgaatggg aaaatctgga agctaaactg aacgaaatgc cgttggctaa
    22201 aaaagacgca cgtacttttg tgcgattctt ctattcggct gccactgaat gcgaacttga
    22261 taaacaagga cgaatcaata tacccagcac gttaagaaat tatgcagctt tgacaaaagg
    22321 ttgcgtagtc atcggtgtat ctaaccgaat tgaaatttgg gatgagacac gatggcaaga
    22381 tttttctgca gcagcagaag agaactttga cgaaattgct gaaagtatga ttgatttcgg
    22441 actataagaa taggaagagg ctgactaatg actgaaacat ttcaacacta tacagtcatg
    22501 ttgaaagaaa cggtagatgg attgaacatc aaaccggatg gcgtttatgt agactgtaca
    22561 ctcggcggtg ccggacatag tgaatattta ttaagccaac taagttctaa agggcatctg
    22621 tatgcgtttg accaagatca aaaagcaatc gatcacgcca agctgcgttt agcttcttat
    22681 gtcgaacaag gtatggtcac atttatcaaa gcgaacttca gagaattaga agaacgcttg
    22741 agtgagcatg tagataaggt agacggcatt ttgtatgatc taggggtttc ctctcctcag
    22801 ctagatgaag cagaacgtgg atttagttat catcaagatg caccgttgga tatgcgcatg
    22861 gatcagtcag ctcctttatc cgcttatcac gtaattaatg aatattccta ccatgaatta
    22921 gtaaagattt tcttccgcta tggcgaggag aaattttcca aacaaattgc tcgtgaaatc
    22981 gaacgagtac gaaaaacaaa accaatccaa acaactggtg agcttgtcga aatcattaaa
    23041 caagtcatcc cagctcccgc aagaagaaaa ggcgggcatc ctgcaaaaag aatttttcaa
    23101 gctgtgcgga ttgcagtaaa tgacgaactc ggtgccgaag aggcctcttt agaacaggca
    23161 atccggttat tgaaagtcaa tggaagaatc agcgtaatca cattccattc attggaagac
    23221 agaattgtca aaagtatgtt taaagaatat agtacggtac aagatctacc accgggactt
    23281 cctgtcatac ctgacgaatt ccaaccagaa ctgaaagtca ttactcgtaa accaatcctt
    23341 ccttcagagg aagagctaac agaaaacaat cgatcacgca gtgcaaagtt acgtattgca
    23401 gaaaaaataa gaatgaaaga ggagtagaat gatggcagaa ttaaagaaaa tgcaagatta
    23461 cgaatatgat ttacctgaat tggtggatca gccggaacaa caaatgcctg attcttcttc
    23521 gaatcaagaa atcattcttc cacaatcacc ggcaaaacga ttaaagcaga tttctgcttt
    23581 ggaaaaaatt gctgtggcaa gcgtggcgat tgctattgtt gctttatgtg ttttaacagt
    23641 tatgctgcgc acaaatatca gcggagctga aaaagaaatc acgtcaatac aaacagaaat
    23701 caatgataaa aagcaagaaa aaacgagttt agaacaagaa aaaaacgaac tttctcgtac
    23761 tgaacggatt aaacaaatcg cagaagaaaa aggattgtct attaatgacg acaatttaag
    23821 gaaagtgaag taaatgagtc ttaaaaataa aattcggcgg tttatggaaa agaaaaatct
    23881 aaatccgatg aacaatcgca aaaaagtagg tatcatttta tttgctacga gtattggatt
    23941 gttcttttta tttgccgtcc gtttctcgta tatcgttatt ggaggtcatg ttgctggtac
    24001 ctcattagct gaaaaaacaa aacagttgta ccaaggaagt gaagtagtaa aagcaaaaag
    24061 aggaacgatt tatgatcgta acggtgtggc gctcgcagaa gatgcctcct cttattcaat
    24121 taaagcgata ctctcaaaaa catatacctc tggggataaa aaactgtatg ttgaagaaaa
    24181 gaattttgat aaaatagcag aaatcctgca taagaatctt tctatagaca aaaaagatgc
    24241 actgaatatt ttagaggacg gcgcaaaaaa agagctgtat caagttgaat ttggtagcta
    24301 tgggaaaaat atctcccaag aaaccaagca aaacatcgaa gcggacatga aaaaagaagg
    24361 tgtcgctggt ctttattttg tcgaccatca ggctcggatg tatccaaatg gcgtcttttc
    24421 ttctcatttt attggatacg cagttccaga caaagatgag aatggcttag ttggtaaact
    24481 aggtttggag tctgcttaca atgatatctt gagcggtaaa gatggtaaga ttatttatca
    24541 aaaagataat tttcaaaatc ctctgccagg tacagtggca gaagaagaaa aagctgtcga
    24601 cggacaagat atctacacga ctttagacag cagactacaa agttatttag aaacactgat
    24661 ggatcaagta aatgaagaat atcagccaga agaattgaca gcagtcctga tgaaagcaaa
    24721 aaccggagaa atcttggcaa tgggacaacg acctactttc aatcctgaaa caatggaagg
    24781 attgactgga aaagatgcta tctggcgtaa tttcttggta caagatagtt atgaaccagg
    24841 atcaaccatg aaagttttca ctactgcagc tgctatcgaa gaaggagaat tcaacgagaa
    24901 tgaaaccttc caatctggaa aaatccaagt agaagatgct acgatcaatg accatgactt
    24961 tggtgaaaaa ggtgtgctga ccatgcgtca agctctttca tggtcaagta acgtcgggat
    25021 ggtcatactg gaacagcgat taggcggacg atggtataac tatttacaaa aactgggatt
    25081 tggacaaagt actcattctg gattagatga tgaagtaaac ggtgctttac ctacattaaa
    25141 tattgtcgac cgagcaatga gtgcttatgg acaagcggta ggtgtgacga atttccaaat
    25201 gatgaaagct ttcacttcta ttgcgaataa cggtacaatg attcaacctc gatatatcag
    25261 caaagtggtg gatccccaaa ctggagaaga gcgaacaact caaacagaag ttctagggca
    25321 acctttttca aaagaaacaa cagaaaaagt ccgtgaatat atgagagatg ttgtcgaaag
    25381 cgaaaactac ggtagtgctt atggtgtata tagtgtacct ggatacaatg tatcggcaaa
    25441 aaacggaaca gcccaaatcg cttctgatac agggggatat caaacaggag atacagccta
    25501 cctgtattcg attgtggaga tggttccttc tgaagatcca gactatgtct tgtatctaac
    25561 aatgaaacac cctaagacat atgatcgaat ggcattggct aaaatcgcaa atccattgat
    25621 gaaacgggca atggatttta aagaaactga agaagattca gatacagaaa caaaaacaga
    25681 aaaagtatcc gtagccgatt atcgtaatct tgaagcggac gttgcagcag ctgatgctca
    25741 aaaaagtgga ctgcaacctg tagtgatcgg taatggtaaa aaagtacaaa aacaatctac
    25801 tgccaacggt gatcagttga tctctggaga aaaactgatc ctatatactg gtggtgataa
    25861 gctgatgcct gacgtaacag gatggtcaaa agcagatatc atgaagctgg ggaaaatcct
    25921 cggtattgaa gttagttttg atggagatgg ttattgtgtc aaacaagaac ttgctccata
    25981 tgaaaaaatc actgaagaca aacttagttt tactttagaa gaataagaag agaaatagga
    26041 gagaaaagag catggaatgg acacaagcat tgattcccat tgtcagcagt tgtgcaatga
    26101 caattgccgc aatgccgcta tttattggtt attttcaaat gaaaaagcaa ggtcaggcga
    26161 ttcgtgagga gggaccaaag tggcacaata gcaaagccgg aactcctaca atgggcggct
    26221 tagtgtttct gatcggctct attttgacag gaatttgggt tggagcttgg caaaaacagc
    26281 taactcctac tcttttcatc ttgctttttg tattagcgtt atacggagta atcggctttc
    26341 ttgatgattt tataaaaata ttcaagaaaa gaaacatggg actgaactcg aagcagaaat
    26401 tgattggaca gattctaggg ggaatcatct tttatcttgt ttaccgttct gaaggttatc
    26461 caggtgtatt gaatttcttc ggactagatc tacctttagg aatcatttat ggtattttcg
    26521 ctctcttctg gcttgttgga ttttcaaatg cagtaaatct gacagatggc atcgatggat
    26581 tagttgctgg cttaggaagt atctcttttg cagcttacgg tctaattgct tggcatcagc
    26641 atcagtatga tgtattaatc atttgcttaa gtgttttagg aggtttactg ggtttcttcg
    26701 tctacaacag aaaacctgcc aagattttta tgggagatgt cggttcatta gcgttgggcg
    26761 gacttttagc ggcaatttct ataatgctaa accaagaatg gacattgctt ttgattggtt
    26821 tgatctatgt tatggaaaca gcaagtgtga tgcttcaagt tacttccttt aagcttacag
    26881 gaaaacgaat ttttaaaatg tctccaattc atcatcactt tgaaatgtgc ggatggtcgg
    26941 agtggaagat tgatacagtt ttttggttga tcagcattgt gacttcgttg atcacattat
    27001 ggatcgtttg gtaggagatg acgggaaatg aaaaaaatat caacatatga aaataagaaa
    27061 gtgcttgtct taggactcgc aaaaagcgga gtaagtgcgg ccaaattatt acatgaattg
    27121 ggcgcacttg tcaccgttaa tgatggaaaa ccttttgacg aaaatccgga agcacaagag
    27181 ctgctttctt tagggataaa agtaataaca ggtagtcatc caatcgaatt gttggatgaa
    27241 gaattttcat tgatggtcaa aaatccaggg attccctaca gtcatccttt agtagcaaaa
    27301 gcccaagaaa tgggtattcc ggtaattacg gaagttgagc tagcttacga agtagcggaa
    27361 tgcccaatta ttgggattac aggaacgaac ggaaaaacaa cgacaacaac gatgacagga
    27421 ctactactga atgcaggagc agatcaagga attgcacgtt tagcgggtaa tattgggtat
    27481 ccagcaagtg gtgtagcgca agaagcaaaa tcagaagata aaattgtgat ggaattgtct
    27541 agcttccaac tcatgggaat cacagatttc cgccctcata tagcggtgat taccaatatc
    27601 tatgaagcac atattgatta tcatggtaca agaaaagaat atgtcaaagc taaatggaac
    27661 cttcaaaaaa acatgacaga aaaagattat ctgatcctta actggaatca gtcagaacta
    27721 caagaattag cgcaaagaac aaaagcacgc gtactgcctt tctcgacaaa agaagtgttg
    27781 gaagacggtg tgtatgctga cgattactca atatactata aaaaagaaaa aataatggaa
    27841 atatctgaac ttggcgtacc aggtaagcat aatgtagaaa atgcactagc tgctatttct
    27901 gtagcaaaat tgtatggtat atcaaatgaa gctatacgag aaacgttgca ttttttccat
    27961 ggcgtaccac atcgtacgca atatgtagga gaaatccaag gcagaaaatt ttataatgat
    28021 tcaaaagcaa ccaatatttt agcgactaaa atggctctaa gtggatttga aacgtctaag
    28081 gtcgtgttat tggctggcgg attagacaga ggaaatacat ttgatgaatt aattccttca
    28141 ttaaaaggaa tcaaagcaat ggtcgtattt ggccaaacaa aagaaaagtt gatggatgct
    28201 ggaaaaaaag caggcatcga gacaatagta acagccgatt ctgtagaaca agcagttcct
    28261 ttagcattag aaaacagtac agatggtgat gtagttttat tatcaccagc gaatgccagc
    28321 tgggatcaat acccaaattt tgaaactaga ggaaacagat tcatggaagc tgtgaaccga
    28381 ttaaaataga aaaagtagaa gcgagcgata aaaaatgaaa attttggtta caggcggagg
    28441 aacaggggga catatttatc cagctcttgc ctttgtcaat tatgtaaaga ctcaagaacc
    28501 gaatgctgaa ttcatgtatg tgggagcaaa acgaggtcta gaaaacaaaa tcgtccctga
    28561 tacaggaatg cctttccata ccttggagat tcaaggattt aaacgaaaaa tttccatgca
    28621 taacatcaag acggtccgat tgtttcttaa gagtatccgt caagcaaaaa aaatcctcag
    28681 tgattttcaa ccagatatcg ttatcggtac tggaggatat gtttccggag cggtagtgta
    28741 cgctgcagcc aaaatgggta ttccaacagt tatccatgaa caaaacagtg taccaggtgt
    28801 taccaataaa tttttaagcc gatatgtgga tcggatcgct ctttcattta aagatgcggc
    28861 ttcctttttt cctgaaaaca aagcagttct tgtaggtaat cctcgagcac aagaagtagc
    28921 agatactaaa aaatcagata ttttgaaaac atttgatttg gatccagaaa agaaaacagt
    28981 cttgattttt ggcggtagtc aaggagcact taaaatcaat caagcagtat cagttttttt
    29041 aagtgaatat attccttctt cttaccaagt tttgtatgct tcaggagata gatattatga
    29101 agagatccta aataaaataa atcctgaaat acaaaatatt agtgtccagc cttatatcaa
    29161 aaatatggca gaggtcatgg cttccagtga tctgctggtg gggcgtgctg gtgcaacttc
    29221 gatcgcagaa ttcacagctc tggggttacc ggcagtgttg gttcccagcc catatgttac
    29281 gaatgatcat cagactaaaa atgcaatgag tcttgttcac gcaggagcag caaaaatgat
    29341 tgcagataat gaactaacag gtgaatcact aagtcagaca gtcaacgaaa tcatgggtga
    29401 tgaagaattg caaaagcaaa tgtgccgagc ttctaaagaa caaggaatcc ctgatgcttc
    29461 aaaacgtctt tatgacttag taaaacaaat aatcaaaaaa taaaggcggt ggtcttagtg
    29521 agtaagaaaa aagaaccaaa aaaattgtct catccgtctt ctgatgaaaa caatttgact
    29581 ccttggcaaa aggctaaccg aaaatatctg gcagaacaag agatgaaaaa ccaagagcca
    29641 aaaaaagaag acataacatt agatacaccc ctttcagatt ctgaagaaga aaaaacggtt
    29701 caagaggaac agcaagaaga aagccagtcc gagaaaaaaa tcgaattttt tgaagagatc
    29761 aagcaaaaga aaaacggtgg accatataac ggttcttttt tgaaccggct gcccaattta
    29821 aaaagtcaac ggcacaaagt actatttcgt cggttagctt tgatcatcac tgtattgggt
    29881 ataccgctga tttttttgat ctattatgtt tctccttata gcaaattaca agcagttgca
    29941 gtatcaggca ataagacagt caacagccaa gaaatcatct ctgatacaaa attgtcatta
    30001 ggagaaaatg tttggggaca gtattttcat cgatctactt atatcgatcg ccttaaaaaa
    30061 gcacagccaa gaatcgaaac agcaaatata catttcaaag ggatgaatga attcgatttg
    30121 gatgtaacag aatataaaga gattgctttg atcgcaaaaa acaatcaata ctacccagtg
    30181 attgaaaatg gaacagtatt agatgaaaaa gtagcaaatc ctacaaaaaa tctccctatc
    30241 ttggaagagt tcaaagactc tgcaaaaatc aaagaattaa caaagcaata caatcagtta
    30301 tcatcagaat tacaaaaagc aatttctgaa atcaagtaca cgccaagagc aagcaataaa
    30361 aatctgatcc aattgaatat gaatgacggc aatcaagtta ttgtaaatat caataatctt
    30421 gcgaaccaaa tgaaatatta ttcgcaagta gccaaagata tggatgaaaa aggtgtgatc
    30481 gacatggaag ttggtatctt ctcgcatcct tacggtagca ctcaagaaac ggaagaatcg
    30541 ggccaatcgg aagaaacaac tgaaagtgaa gaaaatgcgg atcaaacaaa cgaatcaagt
    30601 gaagaggcag aaacgcaaaa agcacaaaca cctggatcgt ctagcacaaa cggatcatct
    30661 gattcaaatg attcaagtga aacaagtcaa atagaagata atccaagtac aactccgtca
    30721 agtttaaatg aaaattgata aaacaacttt aaaaagtaag aatgtacttt ctaaaaacca
    30781 gtaatttatg gtagaattgg aagcagtgaa tatatctatt aataagtata ttttaaaacg
    30841 gacgctagtt gttcgcttgt atatggaata gtaagtagga gggaatccca ttcatggcaa
    30901 aaacaggaat gtatgtaggc cttgatattg gaacgacatc tgttaaagtt gtcgtggctg
    30961 aatatatcga cagccaaatg aatattattg gagtaggaaa cgcaaaatca gaagggatta
    31021 accgaggcat tatcgttgat atcgataaaa cggtccaagc gatacaacga gcagtacgac
    31081 aagcagaaga aaaagcaggt atccagatca aaggcgtaag tgtcggtttg ccagcaaaca
    31141 tgcttgaagt agaaaattgc cagggaatga ttgcagttaa cggagattca aaggaaatca
    31201 ccgatgaaga tgtcagaaat gtggcttcag ctgcccttgt tcgttcaata cctcctgaac
    31261 gtcaaatcgt gtcaatattg ccacaagatt ttactgtcga tgggttcgag ggaatcaaag
    31321 atcctcgtgg aatgatcggc gtaagattag aaatgtacgg tctgttgttt acaggaccta
    31381 aaacgatcat ccataacata cgtaaatgcg tggaaaatgc cggattaatc gtcaacgaaa
    31441 tggtgattac tcctttagcg ctgactgaat caattctttc tgacggagaa aaagattttg
    31501 gtacgatcgt cattgatatg ggtggcggcc aaacaacaac ggctgttatg catgataagc
    31561 aattgaagtt taccaatctt gaccaagaag gtggcgaatt tgtcactaaa gatatctcta
    31621 ttgtattaaa tacgtcattt aacaatgcag aagcgttgaa aataaactac ggggatgcat
    31681 acccagaacg gacatcacca gatgaagaat ttccagtaga tgtgattggt cagtcagaac
    31741 cagtgaaagt cgatgaacgc tacttgtcag aaattatttc agctcgaatg gaacagattt
    31801 tcaacaaagc aaaagatgca ttggaccaaa tcgaagcatt agagttgcca ggtggcgtcg
    31861 tattgacagg cggagctgct agtcttcctg gtgttgtgga cttggcacaa gaaatcttcg
    31921 gcgtaaatgt aaaattgtat gttccaaatc acatggggct gagaaacccg gtatttacaa
    31981 atgtaataag tatcgtggat tattcagcaa acttgagtga agtgtatcaa ttggcaaaaa
    32041 ttgctgttac gggtgaaacc tcagcagctc gtcaaatggt agttgaacaa gaaacaacaa
    32101 acacatatga gagctatgaa gcacctgaag aaacaatcta cgatgagccg gaaccaaaag
    32161 agtccggaga aaatgtaaaa aataaaatca agggtttctt tacaaatatt tttgattaat
    32221 tcatcctggg aggaataaga tatggaattc tcaattgata ataatatcaa tgacggcgca
    32281 gtaatcaaag tcatcggagt cggtggtggc ggtggaaatg ctgtaaaccg aatgattgaa
    32341 gaaaacgtta aaggtgttga attcattaca gccaacacag acgtgcaagc attaaaaaat
    32401 tcaaaagctg aaacagttat ccaattaggc cccaaatata ctcgtggttt aggtgctggt
    32461 tctcagccag aagttggaca aaaagcggca gaagaaagtg aacaatcctt acgcgaagct
    32521 ttagacggtg cagatatgat tttcatcact gcaggaatgg gtggaggaac cggtacaggt
    32581 gctgcaccaa tcgttgcagg tatcgcgaaa gaattgggcg cattgactgt tggtgtcgtg
    32641 acacgtccat tcacttttga aggaccgaaa cgtggccgtt ttgctgcaga aggtatcgct
    32701 cgtttgaaag aaaatgtgga tacattatta attatctcaa ataaccgttt attagaagtt
    32761 gtagataaaa agacaccgat gcttgaagct ttccgtgaag cagataatgt tttacgacaa
    32821 ggtgtacaag gtatctctga tttgatcaca gcgccaggtt acgttaacct tgactttgct
    32881 gacgtgaaaa cagtgatgga aaaccaaggt acagcattga tggggatcgg tgtggctagt
    32941 ggagaagatc gcgtagtaga agcaacgaaa aaagcaatct cctctccatt gcttgaaaca
    33001 tctattgatg gagctgaaca agtacttctt aacatcacag gcggtttgga tatgacattg
    33061 ttcgaagcac aagatgcttc agatatcgta gcaaatgcag caactggcga tgtaaatatc
    33121 attctaggta catctatcaa tgaagaaatg ggcgatgaaa tccgtgtgac ggtcatcgca
    33181 acaggtatcg atgaatcaaa aaaggaacgc aagtctagcc gaccagctag acaagcacaa
    33241 atgcaatcac ctgctcaaaa gactgtatta gatatggatc aagctaaacc aacttcttca
    33301 gaagaagaaa acagttttgg cgattgggat atcagacgtg aacaaaatgt tcgtcctaga
    33361 gttgatgatt ccaattttga caatatcgaa aaaaaagaat ttgatacatt caaccgcgaa
    33421 gaaacaaaat ctcatggcga tgatgaatta aatacgccac cgtttttccg tcgtaaaaaa
    33481 taaaggagag gaagagcaca tgattgctga caacttgcag aaaatcaagc aagaaattca
    33541 ggattcgtgt gctcttgttc atcgtgatcc gaaagaagtt acattagtag ctgtaacaaa
    33601 atctgttgaa tccgctcaag ctgaagaatt ggttagagct ggcgttatga attgtgcaga
    33661 aaaccgagta gataaattat tagaaaagaa acaagattta tttgattttc ctgaaataaa
    33721 gtggcattta attggtaatt tacagcgcag aaaggtaaaa ttgattgtaa atgaaattga
    33781 ttactttcat gcgttggaca gtttaagatt agcagaagaa atccagaagc ggttagagaa
    33841 agaattatcc tgttttattg aagtaaatgt ttctggtgag gaatcaaaac atggaatttc
    33901 accagatgag cttttaccgt ttgtggaatc tttagctgcc tgcacaaaaa tcaaggttgt
    33961 tgggttgatg actatggcgc ctaaagaagc ttctgatgag caaatccata aagtgtttgg
    34021 tacgctgaaa gaattgcgtg atcttgttca agcaaaaggt tatgcccatg caccatgtag
    34081 tgaattaagt atggggatga gtcaagactt tcaaattgcg attgaagaag gtgcaacatt
    34141 tatccgtgta ggaacagcgc tattcaaaga cgaataaagg aggaacaaaa atgccgcttt
    34201 taaacagagg agcaattgct aattttttcg gattagctga tgaggaagag tacgaatacc
    34261 aagattatcc tgaaacaaca aaaaaggctg tacagccaac taaaaaaaat acaacacaga
    34321 atcaatcaac tcattctgta tctgctgcga atactcatga aaaacaaaaa acggcgactt
    34381 cacgtcctgt aacgcatgaa agtattcagc cggctgaaaa gaaaaaagta attgaactac
    34441 atcaatcacc tacaaaaact gtagcgaaag aaagaacagc ttcaagaacc tctacacaac
    34501 ctaaagcaaa tcttgctgga aaaatcactg taatggaacc aagagcttat tctgaagcta
    34561 tggcgattgc taaacgaatc attgctggag aagcaacctt agtgaatttt catttagtag
    34621 aagaaaaaca agcgagaaga atcgttgatt ttctaacagg aacagtttat gcactagatg
    34681 gagatatcaa acgtgttgga gatgagattt ttctttgtac accgactggg atggagattg
    34741 acagttcaac cgctcaatct tttgcagatg cgaatttgtt tgatctgtaa gaggaggaac
    34801 tacgatttat actattgcac tattacttca tttacttcaa cgggcagcct atttctatag
    34861 cattatcttg atggtatatg cattgctctc ttggtttcca ggcgggtatc aatcagggct
    34921 aggacgatta ttagctaaga tctgcgaacc gtatatcagt ttgttcgatc gtctgccttt
    34981 gaggattgga cccttggatt tcacaatttt aggggcaatc ctgatcttga acttagccgt
    35041 acaaggttta gcacagatcg ttatatggat aactgtccat ttagtttatt gattgaagca
    35101 acaaaatctt tattctcaga atagatagga ggggaaaatt ttggatgcaa atgtatacca
    35161 gcactttcgg gcagatgaac gtacatttat cgattcggta ggcgattgga ttgaacaggt
    35221 tcaaggacaa tatgcaccct atctgaccga atttcttgac ccaagacaat cctatatttt
    35281 agaaacactg attcgacaaa gcagtgattt gcgtttcatg ttttatggcg gatatgaaca
    35341 ggcagaaaga aaaagatgtt tgattttccc cgattattac gaacctgtag aggaagattt
    35401 tgaaacagca ttgtatgaaa tccattatcc tagcaaattt gccagcctaa gtcatgggaa
    35461 aatccttgga actttagtgg gaacggggat caaacgctct tactttggcg atatcatctc
    35521 ggacggtgat cgctggcaag tatttattgc aaatgaggtg gatcattttg ttgctgcaca
    35581 agtaacaaag attggaaagg ttgctgtacg cttggaacca atcaaataca cagaaatgat
    35641 tatgccgaaa gatagctgga cacaagaaag aggaacagtt agttccatgc gactagacag
    35701 tgtgatctct gagatttata atatttccag acagcggtcc aagcagctga tcgaagctgg
    35761 taaagtaaaa gtgaactgga cagaaaatac aagacctgat tttgtacttg aactattaga
    35821 tattatttca gtcagaggtt atggcagagt ccagatccaa gaacttgagg ggaagacgaa
    35881 aaaagaaaag tacagggtac tgttcggtgt attacgtaaa taaaataaaa aggtggttta
    35941 ctcatggctt taactccatt agatatccaa aataagacat tcccgacaaa aatgcgggga
    36001 tataatcaag acgaagtaga tgatttctta gatttagttg ttcgtgacta tgaggaattg
    36061 actcaaagaa atcgagaatt agaaaaagca gtcaaacatt ctgaagaaaa acttgagtac
    36121 ttcaatgaat taaaagatgc tttgaaccaa tcaatcatcg tagctcaaga tacggctgat
    36181 aaagtaaaaa caagtgcaag caaggaatcc gaagtgatcg ttacttctgc acaaaacaaa
    36241 gcagatgagt tagttgcgaa cgctgaaaaa cgtgcacatc aattaacaac tgatgcagaa
    36301 gaaaaagcaa gaaaaatctt gacagatgca acagaaaaag caagacagct tgcgacagaa
    36361 actgaagatc ttaagaaaaa aacacgagta ttccatcaac gtatcagctt gatgcttgag
    36421 tctcaattag aacaagtgaa aagtccggaa tgggatgaaa ttttacaacc attctccagc
    36481 tacgtgactg acagccatga agtgatcaaa gaagttttag caaaacagct tgacaatgaa
    36541 aatgatactg atgtaaactc agacacagga gtcatagaag cacctatgac ggcatttgat
    36601 acacaggcta ttgatatctc gatcgtgcct ggtgaaaatg aaaacgagta gaacagaaaa
    36661 gcactttgtt attcttaaag cgagccgatg agagtggaag ttcggtaaga cactgcatgg
    36721 tgaaagatcc tctacttgag acctgctttt ccgaaattag agtaagaagc agcggctgac
    36781 ctcgttattg gtcacagaga gtttgcacta gatagatgca actaaatttg ggtggtacca
    36841 cggcatgttc gtcccttgcg gctgaacatg cttttttgtg tccggaaaac aaataaagga
    36901 gtgtatggtt gaatgaaaat gaaagaaacc ttgcaattag gaaaaacaaa attccctatg
    36961 cgtggtaatt tgccaaaccg agaaggcgag tggcaaaaag aatgggaaga aaatcgaatc
    37021 taccaaaaaa gacaagagtt gaacgaagga aaacctagct ttgttcttca tgatggacct
    37081 ccatatgcca atggtaatat ccatttagga cattcattga ataaaatcag taaagacatt
    37141 atcattcgtt caaaatcaat gtctggctat cgtgcaccat atgttccagg atgggataca
    37201 catggattgc cgattgaaca agtattggct aataaaggcg tgaaacgtaa agaaatgtcc
    37261 atggcagaat acttggaaaa atgtcgtgaa tatgcacttt cccaagtaga taagcaacga
    37321 gcagatttca aacgcctagg agttgcagga gattgggaac atccttatgt tacattagat
    37381 ccttcttatg aagctgcaca agtacgcgta tttggtaaaa tggctgaaaa aggctatatt
    37441 tacaaaggat tgaaaccaat ttactggtct ccgtcaagtg agtcttcttt agcggaagct
    37501 gaaattgaat ataaagatgt aaaatctcca tctatttttg tagctttcaa tgtcaaagac
    37561 ggcaaagata ttcttgatga agatacagct tttgtcattt ggacgacaac accatggaca
    37621 ttgccagcaa accaaggtat tgctgtaaat ccaaaattta cgtatgtatt ggtggaagcc
    37681 gatggccgta agtttgtcgt agcgaaagat ttgctagaaa cagtccaatc agaaatcggt
    37741 tgggaaaatg taacagttct aaaagaattt gctggacaag aaatggaata tatgactgca
    37801 agtcatccat tctatgaacg tgaatcactt gtgattttag gcgatcatgt tactttagac
    37861 gctggtactg gattagtcca tactgctcca ggacatgggg aagatgacta tatcgctggt
    37921 agcaaatata aactatctgt agtgtcacct gtagactcaa aaggattgtt tacagaagaa
    37981 gcacctggat ttgaaggtat cttttatgac aaagcaaatc ctatgatcac tgatctatta
    38041 aaagaaaaag gagctttgct gaagctagat ttctttactc atagttatcc tcacgattgg
    38101 cggacgaaaa agcctgtgat ctatcgggca actccacaat ggtttgcatc tatcgataaa
    38161 ttccgtcaga atattttgga tgaagtagaa aaagtagagt gggttatccc atggggtaaa
    38221 acacgtttgt ataatatgat ccgtgaccgc ggcgattggg taatttcccg tcagcgtgca
    38281 tggggagtac cacttccgat tttctatgct gaaaatggag aagcaatcat cacaccagaa
    38341 acaatcgaac atgtggcaaa attatttgct gaacatggat caattatctg gtttgaaaaa
    38401 gaagcgaaag aacttcttcc tgaaggattt acccacccag gttctcctaa tggcgaattt
    38461 acaaaagaaa aagatatcat ggatgtctgg tttgattctg gttcttctca tgaagctgtt
    38521 ttacgtcagc gaccagaatt atcattccca gcagatatgt atttagaagg atcagaccaa
    38581 tatcgtggct ggttcaactc aagtatcacg actagtgtgg ctatcaatgg tgtagcacca
    38641 tacaaatcag ttatttctca aggatttgct ttagatggtg aaggccgtaa gatgagtaaa
    38701 tctttaggaa acatcattgc accagaaaaa gtgatcaaac aatttggtgc agatatcctt
    38761 cgcttatggg tatctagtgt agattatgaa gcagatgttc gtgtttctat ggatatctta
    38821 agtcaagtgg cagaagtata tcgtaaaatc agaaatacga tgcgtttctt attagcgaat
    38881 acagaagatt tcgatcctaa aaaagatact gtttcgtaca atgatttacg ttctgtagac
    38941 aaatatatga ccgttcgatt gaaccaagta atcaaagaaa tccgagaaga agggtacgac
    39001 aaatataact tcatgcatat ttaccgtaca gttatgaact tcttgacggt tgatctatcg
    39061 tcattctacc tggattttgc taaagatgta gtttatattg aagcagaaga tgattatcaa
    39121 cgtcgttgca tgcaaactgt tttctatcaa acagctgtag ctttgacaaa attattgact
    39181 cctatcattc cgcatacagc agaagaaatc tggtcattcc tgaaagaaga agaggagtac
    39241 gttcagttaa gcgagtttcc tggttatgaa gagtttgcaa accaagaaga attgatggat
    39301 acctggactg ctttcatgga cttccgtgat aatgtcttaa aagcattgga agaagcaaga
    39361 aacagcaaat tgattggtaa atctttggaa gcaaaactaa ctgtttatcc aaaacaacaa
    39421 gttcgtgaaa tgttaaaagc tttggatgca gatattgcac aattattgat cgtttcacca
    39481 gactactttg aagttatggc agcagatgaa caagtgccag ataacgctat ggtatttgat
    39541 gatgttgcta ttacagtgga aaaagcagac ggggaaactt gcgatcgctg tcgccaagtt
    39601 cgtaaagatg taggagtaga tgaaaaactt cctcatctat gcggtcgttg tgcaaaaatc
    39661 gttgaagact tctatcctga agctgtagct gaaggattcg aagaaaagta aaagggaaaa
    39721 gggggtgtga catgttcttt gtcacacccc cttttgttat caatcgttta attttccacg
    39781 ttcacgatac cagttatctg gttgccagat ccattcgaat ccgtcttttt caagtagttc
    39841 gaaagctgct ttaggtccca ttgatccagc tttgtagttg ggaaaatccg gttgtgtttg
    39901 gtcccaagct tttcgaatga catccacgat atgccaagat tgagcgactt cttcccaatg
    39961 agagaagttc gttccatcac cattcaacgc atctaatagt aatttttcgt aagcttctgg
    40021 gctattttca accatttctg cactatttct aaattccaat ttgattggtt ccgtttcaaa
    40081 tccttgccct acttctttcc cattcaagga taaagaaaat ccttcagttg gctgaatata
    40141 gatcgttaga acattaggtg gaagtgtcgt atcgtcacat ggttcatcga cagatgtttt
    40201 gaatacatta acaggaactt gtttgaatac gatgttgatc cgagtccctt tttcagtcaa
    40261 acgtttgcct gtacgtacat agaaaggaac acctgaccaa cggaagttat cgatagtgaa
    40321 tttgcctgcg acgaatgttt cagtctctga ttgagcatcc actttgtctt cttcgcgata
    40381 gcccttgaat tgtacaccat ccaattcacc ttgagcatat tgcccgcgaa cgaagttttc
    40441 taacacttct tcttcgttat aaatgcggat agctttcaat gctttgatct tttctgtacg
    40501 gatttctttt tcggaaaatg ctactggcgg ttccattgca agtaatgcta caacctgtaa
    40561 aatatgattt tgcaccatgt ctttaagtgc gccactatga tcgtagtagc ccccgcgatc
    40621 ttctacaccg aggttttcgc caaaagtaat ttggatattg tcgatatagc gattgttcca
    40681 ttgagcctca aaaatattat tggcaaaacg gatagctgag atgttttgga tcatttcttt
    40741 tcctaaataa tgatcaatcc tgaaaatatc ttgttctggg aatacttctc tgatttgctg
    40801 gttcaattca aaagcagact ggtaatcaga accgaacggt ttttctatga tcaaacgatt
    40861 gaagccattt tttgtcataa tattttgtga tttcaaatga gaaacgatcg ttccaaaaaa
    40921 ttgaggtgcc atagccaaat aatagacacg gttaccgttt aattgatagt gtccatctaa
    40981 tcggtcagac aattctttta atgtctgata atgcgttgaa tcatttacat catgggattg
    41041 atagtaaaaa tggcttgcaa aggtagttgc ttcttcatct gaaggattca atgcacggat
    41101 tgtttctttg acaacttcac ggtagtgctc atcgctccag ggacggcgag cggttccgat
    41161 tacagcaaaa tgctcgccta aatttccttt tctgtaaagg cggaacaaag acgggtacaa
    41221 tttacgctgg gctaggtcac ctgtaccgcc gaatatcgta aacaatacat ttttttcatt
    41281 agacatgtgg atacactcct ttatatttta tcgacaaaaa tcgtgctggc cgccttaaag
    41341 ctgacgggga ttttctttct atcattttgg atagtgatcg gtccttcata agctgcgatg
    41401 tcaacgatct catattcttc gttgatattc aggtcaatag tgactaagta atccaacaat
    41461 tctttttcat caagcactcg tgcgatacgc acttttgtac cgacaggata ggactccaat
    41521 gtttgtcggc gttcttcatg gacaacttcc tcttttttag gaatcgatcc tccatgagga
    41581 caatgttcag gatgattcaa atagttttct aaatgatctg caagatgttc cgaagtcaca
    41641 tgttccaata cttcagcatc atcatggact tcattccagc tatagtctaa atgttcaact
    41701 aaaaaaactt cccataagcg atgtttgcga atcagactgc tagctttttg cagtccgctt
    41761 gcagtcaatt gtacaccttg ataaggggaa tgttctacaa gctcttcttt cacaagttta
    41821 gaaatcattt cactgacaga tgcagctgag acatctagac tagatacgat ttgtttattg
    41881 ttgactttcg ttgtatctcc acctagttct aaaatgattt tcaaataatc ttctcgattt
    41941 ggtgtcatat tcgatcatcc tttcatggta aaaaaccatc attagtaagt gtaacaaaaa
    42001 aaacgttaat tgactatttt tctaaataaa aaacaaagga tttttagacg tttttacaac
    42061 aaaaaacgaa agatgataga gaagactcat tctttcgttt ataaaggata tatatataca
    42121 ttgggaggga tctatttttc agataacaac ttatatgaag tgtctgcaga ttcgtcataa
    42181 agagtttgtt cgggataaga aaagataaga taaaaaccat actgatcacg tacgactaaa
    42241 cgatattcat ctatttctac cacagatgtg ccaagagact gaccgttgat caaaaacgtg
    42301 taatcagatg taatctgtaa ttgttgtttt tgctggctat cactaaagaa ccatgtacct
    42361 tgatactttg agagtccttc tgacttttcc gcttgttttt tctttttcaa gtaaataaaa
    42421 cttgagagaa cggtaatgag tgaaccaaga aaatagatca ttttttttat caccaaatca
    42481 ctcctttatg agaaaataat cgcatagaca tattctttct tctttatttc attgtaccga
    42541 ataaaagcta aaatttctct aagactttcg actggttttt ttactgtttt tctacaaatt
    42601 atgataggat acatagtagg aagaaaggtg acataatttg aagtatcagc aagtggaaat
    42661 agcttttttt attgaccgcc cgaatcggtt tatagcacat tgtatgaaaa aagacggaga
    42721 aataattatt gcgcatgtca aaaatacagg tagaggaaaa gaggtcctga taccaggcgc
    42781 tgaagtggct attgtatttg ctcctggacc taaacggaaa accgcctatg atctgattgc
    42841 tgtaaaaaag aagggtgact ggtttaatat cgatagtcaa ttgcctaatc ggctggcaat
    42901 tgatggtatt ttagacggca cgatcctttt accaggatta actggtccat ttacggtata
    42961 caagcgtgaa gtaacttatc ttcactccaa atttgatatt tatttagaga cggtggtcgg
    43021 acaaaaagca tttgttgaag tcaaaggtat gacacttgaa aataaagcga ttggtgcttt
    43081 tcctgatgcg cccacactca gaggattgaa acatatagga gaattaacgt atgccgcaca
    43141 agatggttat gccgcttatg tcttgtttat tgctcagttc gaacatcttc atctggcaac
    43201 gatccatgaa gaaatgcaac cagcactagc tgatatggtt cgacatgcgc agcaaagcgg
    43261 agttcaaatc ctcgcctata attgtcaagt atcggcagat gaggtaactg tcaagcaggc
    43321 cattccattt gatgtagata tcccttttga agatcctata caatagaaag aaggtaaaat
    43381 aatgatcaac ttaggtatta tcggaacaaa ttggattact catcaatttg tccaagcagc
    43441 tttaagcaca aaaaaatatg aattgacagc ggtatattct agacatttgg aaaaagcaca
    43501 ggaatttggt tctcattatg aaggggatat cgaattcgca acagatttga agactttttt
    43561 caatattact catatggaca ccatttatat tgcttctccg aatagtcttc actttagtca
    43621 agctaagcaa gcaattttgg caggaaaaaa tgtgatagtt gaaaagccag ctttttcgac
    43681 gccaaaagaa atggatgaaa tcatccattt ggcaaatgaa cacaatgtgt atttttttga
    43741 agcagcaaga aatatccatg aatcaggttt taaaaagatt gccgattact taccattaaa
    43801 agacgagata atcggtgcaa atttcagcta tatgaaatat tcttcgcgct atgaccaagt
    43861 attagatgga gaagaaccga atatcttctc gactcatttc tcaggtggtg cattgatgga
    43921 tttaggtgtt taccttgtat atgcagctgt tgcttggttt ggaatgccaa aagaagtcca
    43981 ttatttccca agaaagattt ctactggtgt agatggtctt ggatggggga tcttacgtta
    44041 tgagaatttt gatgtatcga tccagccagg taaaatcggt gattcttatt taccgtcaga
    44101 gatttatttt gactcaggaa cgctagtgat gaatggagtc aatgcaattg aacaagcaca
    44161 atttcatgat cgcgcgcata aagaaatcat tgatttggcg gtaacagctg cagaaaatcc
    44221 aatggcagat gaagcagagg attttgccaa tgtcatggct catccgaaag atccagcttg
    44281 gggattgttg tatgaagagt ggatcgagct agctagaaat gtgaaccaag tgatttatga
    44341 tttaagaaaa aatggcggca tcaaattcga tgcagataat gaggaagatt tttaatgaac
    44401 gaatttgtac aagtatttcc cgaaaattgg cagaaaaaat atgaggataa agggtttatc
    44461 tcaccttctc ttattcaacg agcagttttt cagccactaa gtaataaaca aagtatagta
    44521 gcggtttctc ctacaggttc aggtaaaaca ctggcttatc tttggcctct actactaaac
    44581 gtggaaccag gtgaagcaag tgcgttggtc atttttgctt cttctcaaga actagctatt
    44641 caagtagcag atgttgcacg tgaatgggga aaagacaaag agttgaaagt ccaatctttg
    44701 gtgggcggag ccaacgtcaa acgtcagatt gagggtttga aggaaaaacc agaaatcttg
    44761 attggtacac caggaagaat attggaattg atgaaagcaa aaaaaataaa agcacatcaa
    44821 gtgaaaacaa tggtatttga tgaagcagac cagctatttg acgctgggaa cagccaaatc
    44881 attgatcaaa ttcttcatca agctccaaca gagtatcagc tagctttttt ttcagcgaca
    44941 gcagatcgtt cccttgaaag cattgaaaaa atcactggga aaacgtttga gacaattgac
    45001 gtgacagcag aggacgattc tcgaaaagga ctgagacatt actttttacg tgtaccgata
    45061 cgaaagaaag atgactactt gagacgtctc acgcatatag aagattttca tggtctgatt
    45121 ttctttaatc aactgactga attaggcgta atggaagaaa aattgctgta tagaggtgta
    45181 ccggtaggaa gtctggcctc tgatcagaac aagctactcc gaaaagcagc gctagaacaa
    45241 ttcaaaaaga aaatggtcag agcacttttg accacagatg tggcggctag aggtttagat
    45301 attacaggtc ttccttatgt agtgaatgca gaagttccgt taagtgaaga agcttatttg
    45361 catcgttcgg gaagaactgg tcgaatgggt aatgaaggaa tagtaattac actcgtacaa
    45421 gataataatc taagagattt aaaacggctt ttacgtccat caaatataac acttgaagag
    45481 atttttttat acggaggtaa gctgcaaact gagcagccaa ccaaagaaga tgcagctcct
    45541 gcagtaaata aaaagtatgc gaaaactttt tctggaaagg agcagaaccc agaatataac
    45601 aaagaaaaaa taagaaaaaa agttaaaaat aaatcaaaaa aagagagaaa taaaggagca
    45661 aggcgcaaat aagcgccttt ttatgctatg attatgaaaa cgggaacaaa attggttgcc
    45721 agaggagggg ttatgtgaaa gaacgtcaag aagagatctt gcagctattg acagaaaacc
    45781 aacaaggatt gacagcaagt gatgtagctg agcgcttaac tatcgatcga agcaatgcaa
    45841 gccgatattt aagtgaacta tacaaggccc atcatattgt aaaaaccgct ggccgaccag
    45901 tcgtttactc tttgccaacg gaaaagtcaa aatctgatga agtgcatgtc gactcttcta
    45961 cgcaagtgac ttttgaaaca ctcgttggag aaaatgattc tttaaaagtc agcatacagc
    46021 aggcaaaagc tgcgattctt tatccaccgc gggggttgca tacgattatt tttggagaaa
    46081 ctggtacagg gaagtcgatg tttgctgaat gtatgtatca ttttgcaatc gattcagaga
    46141 tgctttctgc agatgcgcca tttgtttctt tcaattgtgc ggattatgct caaaatccgc
    46201 aactgctctt tggacacatc tttgggatca aaaaaggggc ttacacagga gcagctcaag
    46261 atagtcccgg tctaatagcc aaagcagacg gcggtatatt gtttttagat gagatccacc
    46321 gtttaccgcc ggaagggcag gaaatgctgt ttactttcat tgataaaggt atttaccgtc
    46381 cgcttggaga aagtgcacaa gtacatgaag catctgttca gatcattggg gcgacgactg
    46441 aatcttcaga aagtttctta tctacgttca atcgacggat tcctatggca atcaccttgc
    46501 cgaatcttgc ttcacgttca ctggatgagc gttatgaaat catctctctt tttatcaaac
    46561 aagaagcgaa tcgtttgaat caacgtatcg atatcgaaaa agaagcgatt cttgccttca
    46621 tgctttatga tgcggaagga aatatcggtc aagtgaaacg agacttgaaa ctagtctgtg
    46681 caaaatcttt tttacattac cgtactcatc atgagaaaaa gctaatcatt cgaaaagaag
    46741 aactttccct tcaagtacaa aaaggattac tgaaaatcaa ggaaataccg gaacgtctcg
    46801 accgattcat ggacagcaaa agtcagtatt tgacatttga accaggattt gctgatgttg
    46861 tctggtcaca agatccagaa cgaaacatgc aggtctataa cgatattgag gaaaaagtca
    46921 cttctctttc cgaaaccggt gtggagaaca ttgatctaga gaccctcatt tcaaaagatg
    46981 tggatgctta tttccagaca tatgtcaaag aattgactaa aagttcgatt cccaaagatc
    47041 ttttgccaga tgaaatctgg cggttaacca atcaattgta tgatgtggct gaaaaacggt
    47101 tagaccgtat ttataatgaa aaagctcgtt ttgcttttgc tttgcatctg caaagtacat
    47161 tggatcgtgt gagagaaggt catatgatcg tccacccaga tttgaataac gtacggaaaa
    47221 attttaagag ggaattccag gtagcaatcg atctttcaac gatcattgaa gaagaagaac
    47281 aagttgagat tccttttgat gaaatcggct ttattagtat gtttctttct atcaatctag
    47341 gagaaagtga gcctgttaaa gaaaacaaag tagatgttct catattgatg cacggcagac
    47401 agactgcttc aagcatgttg gagatggcac aagaactatt aggaacaaaa gtagggaaag
    47461 cttttaatat gccactggtt atggaagttc aaacaatgta tgaagaagtg ttacaatatg
    47521 tcaaaaacta tcaagagcat ttgacgaatg gcctactctt gttaacagat atgggatcat
    47581 taaacacctt cagtgatttg atttatgaag agactggcat tcggacgaaa gctatcacga
    47641 tgacaagtac catgattgtg atagaagcga tacggatggc aagtgttgga agaagcttag
    47701 aagatattta tcaaaatatc cagctttcct ttgaatcgat cgtacgtgat cagttcaaag
    47761 cggaaaagtc tgaaacttta cagcgtaaaa aagccgtaat cgtgacatgc tttactggtg
    47821 aaggagtagc tgccaaatta tatcagcgta ttatgccagt gattaatcaa aataaggtag
    47881 aactgatcca gatgcagttt ttagaacgtg aatcattcaa aaaacacatc gatgccttat
    47941 tggaagaata tgagatcaaa gcaattgcgg gaacagttga tgttgattat caaaatatcc
    48001 catttttttc agcctatgac gtttttgatg atgaaaaact aaatatttta aagcgtatag
    48061 ctgatgatga gatcccgaca gctaaaattg tcgattcact tgctggatca ttaagcgatg
    48121 tcttttctat tgatgaactg atccgttcat tgcaaaagac agttcagcag atccagaccg
    48181 atttgtcgat tatcgtggaa cctggagtag atactggaat cgttatccat ttagccttca
    48241 tgttagacgg attgatcaaa caagaaagcc atcggcaatt tgagaaactg tctattttct
    48301 caaaaattta tcgtttggaa atggatatcg ttcgggcaaa cttgatggca ctagaacgaa
    48361 aatatcaggt aaaaattcca gaagatgata tcgcatttct tacacaaatg tttcttgaaa
    48421 ataaaataaa aaaatgaaca cactaatcat actgtttaca cactaaacat ctgtgtgcgc
    48481 actataaaaa agagctaaaa agcatgaaaa cgctatttca gctctttttt ttattggcat
    48541 gcttcttgcg ttatataaaa gtgtaaaaaa ataaaaggag tgttaaaaat ggcaaaaaaa
    48601 acaatcatgt tagtttgttc agcagggatg agtacgagtt tattggtcac aaaaatgcaa
    48661 aaagcagcag aagaaaaagg gatggaagcc gatatttttg cggtctctgc ctcagatgca
    48721 gataataatt tagaatctaa gaatgtcgat gtattattat taggtcctca agtacgtttc
    48781 atgaaagctc aatttgaaca aaaacttgcg cctaaaggta ttccattaga tgtcatcaat
    48841 atgcaagact atggcatgat gaacggagaa aaagttcttg cacaagcaga aaatctaatg
    48901 aaataaatca agtatgcagt tgggggtata acaacgtgga agatcaacaa aatctagaag
    48961 ccatcatggg gttaatcatg tttggcggta atgcaaagag cgatgcaatg gaagcgattg
    49021 cggctgctaa aatcggtgat tttgagttag cggataggaa aatcgctgat gcagaagaat
    49081 cacttataca agcgcatcat gcacaaacag acatgttgac acaagaagca caaggaaatc
    49141 atatgcaagt gacattattg actgttcaca gtcaagatca cttaatgaca tctattgcgt
    49201 ttactgattt agcaaaagaa atcatcgaac tttatcgtcg cgtatacagc gaataaatac
    49261 aacttctttt ttgaacggaa gaagaaaaat ttttgaacgg attttttatt ttgttataag
    49321 gagaactctc tttatataaa aaacttatag gaggaagtaa aaaatgaacg gactatcaag
    49381 ttggatggag aaatacatcc tgccggttgc cgcaaaaatc ggagcgcaaa aacatttagt
    49441 tgctttgcgt gatgctttta tcggcatact gcccgcaaca atggcgggat cagtcgctgt
    49501 aatgatcaat gcaattattc gtgatttacc gcaacagttt aacagtacct atgctgacag
    49561 tttagcagca gacaaaggaa tctttgccgt attgaatgtc atcatcggaa tcaatggttt
    49621 tgtctggaac ggtacactag caatcgccgg attgatcttt gccttttctt gggggtataa
    49681 tcttgcccga gcttatggag taaatgatct agctgggggg atcgttggtt tagctacctt
    49741 gattcaaggt atttcctttg gtccagcatt atcagcagca ttagatgtga atgttccttc
    49801 aaatgtcgca gatgcaatca atagttctga gattggtgca acgattgcag atggcacact
    49861 gtcagttgga ttatggggtt ggctaaaact agatcatttg aatggaaacg cttattttac
    49921 agttatgatc atgggagcta tttcagtcat tattttttgt aaattgatgc ttgccaacat
    49981 tacgatcaaa atgcctgatt cagtaccacc agcagtatct aaagcatttg cagccatttt
    50041 accagcgacg atcgctttat acgtcattgc tattatcaat tatatcgtag gtcgtgtagc
    50101 tgatgggcaa ctgatcattg atttggtgca aaaatatatt gcagaaccat tcctaggatt
    50161 atctcaaggg ttgggagcag tattgatcgt cacagtattt gttcaagtat tctggttctt
    50221 tggtattcat ggatcaaatg tattggcacc agtgctagag ggaatctggg gacaagcaca
    50281 actaatcaat attgatattt tccaaaaagg ttataacgga aaaactggta caccagccgt
    50341 gttagatgca atcgaagatg gaaaagctta tatgtgggtc cgcggatcgt tcgatgcttt
    50401 tgcatggttt ggcggatcag gcggaacgat cgtcttactg atagctatct tgattttctc
    50461 taaacgtgca gattatctaa cagtagctaa attatccatt gggcctggta tcttcaatat
    50521 caacgaacca gtgatgtttg gtctgccaat cgtattgaat gcaatcatgt ttatcccatt
    50581 cttgattgct ccagtagtag caacagcaat tggatggaca gctacttatc ttggcctagt
    50641 cgctccggta tcgcagcaag tcacatgggt agtaccgccg ttcttattgt cattcttagc
    50701 aacaggagcg gattggcggg caccgatcgt aacattagta tgtatggtcg tgactttctt
    50761 gatttgggca ccatttgtca tctctgcaaa caaaatggac cctgccgcag gcgaacaaca
    50821 ataagatgaa cgtgaggagc gggacgcttt aagtttcgtt cctcataatg aattttcgag
    50881 gaggaactga aatgattaca attgccagtg caatgcaaca agatgaaatc aaagaattgt
    50941 tagaaggata caatgaagaa ggaatgactt ttacatttaa agaaaaacaa ggaatcaagc
    51001 tgttctttga aacaagtgca gaagatccgg aagttgcggc taaaaaagca aaggaactaa
    51061 tcaaagcaca gccttgggga acagtccttt attttcaatc aacagctgtt tagaaaatgg
    51121 tttaaaaaga taggatggtt gagatgataa aaaacggttt atttttatgt agtattggtt
    51181 ctttgatcgt gttgtatagt ttgaatccac attcgcaaag gtatgatttg tatagtatgg
    51241 tcacaggtct ttttctaaca ggattaggaa tctatttctt catgaagggg aaaaaaagag
    51301 aagaagcaaa ggagaaaaat aactaatgga aatcacgaaa aaattaaata ttccagcatc
    51361 gtttttttac gataaagtta tggactctgt ccttttcgat gtacgtaaag cgaccggcaa
    51421 atcagtaact agaaaacagc tgaaaaattt ggagtacgtc aaacaattct cgcaaaataa
    51481 ccgggctcgc ataaaaatcg aagaagtcat tgaaaaccag tcttataagt ttcgtacatc
    51541 gacaacgaag aatgaattcg tcgttcatta tcaaatcaaa ccattggacg attcgacctg
    51601 tgaggttcat tacacagaaa aaatggagtc ttatggcatg cttcaaaagt tgaatgatat
    51661 gcttttagga acaatcttga tgtattttaa acggagacgt ttcaaaaaaa tgctagatat
    51721 gatcgaagca acgtattaaa tcaatgaaac atggtactat ttagttattg aaaaatgaag
    51781 taagctagaa ggaatttttc ataggaagat agatctttcc tgcgaatcct cactagcttt
    51841 tttgtaggaa ggggcagcaa aatgacaaac aaagtaagag agctaggtgt atcttcgatt
    51901 gaggaaatgt ttgatttagc cacttatgca tttaatgggg cagatacacc tgaaaggcga
    51961 gaacgttttc gtacacttgc ggagaattct tggaattacg gattttttga tgaggaggat
    52021 cggctaacaa gtcaagtcat ggcgacccct tttccagtga atttttacgg acaagagtac
    52081 ctaatggccg gcattggcta tgttgcttct tatccagaag caaggggaca aggcgggatc
    52141 aatcagatca tggaaaagat ccttgaagat tgccgtgatc gtaaagtaag cttatcttat
    52201 ctggcgccat tctcctatcc cttctatcgg cgctatggct atgaacaatc atttgataaa
    52261 atcagttatc aactaaacag tcgtgatgtg ccgtttatca aaaaaaaatc tggaaaaata
    52321 aagcgtatgg attttgaaga tgcaaaagct gcaatcagaa gaatttatga acagatgccg
    52381 gaaaaccaac gagctggtct gaagcgcgaa gactggtggt atacgtataa attcaagcaa
    52441 aaaggcaatt accagtttgc tttgcatttt gatgataatg aggaagcaac gggatatatc
    52501 gtttatcagc ttgcaacgtc ttcctttatt attgcggagt ggggattttt ggatcatgat
    52561 gctttttgca gccttgttcg cttcgtaagt tctcacaatg gtgcttttga aacatttgaa
    52621 tattcatgcg gctacggcgg taatgatcaa aattatctat tagaaaatcc atttgcttct
    52681 gtaatgatca caccttatat gatggcgagg atcgtagata tttctttgtt tttggaaaat
    52741 tatccattta aaaagagaca agctgcattt gccctacaaa tagaagaaga tcattatgcg
    52801 aaatggaatg aaggaatcgt ggaagtaaca atcgaaaatg gaaaaaatca tctctcaaaa
    52861 gtagaacaaa caaaactccc aatcgttcga ggaagtatcc aacatatcac gcagctatta
    52921 ttagggtatc gtaaagtgga agagttggta ttccaagaaa gaatacaggt gacaaaagaa
    52981 tatgaagaaa cgttaagctc aatcttgcca cagcaacagc caattcttaa tgactatttt
    53041 tgataaaaaa cgggtgatga caatattttt gtcaccaccc ttttgttttt catttggcat
    53101 ttgaaggaaa atgttaaagt aaatcctctt ttttcgtaaa tttgaatttt ttgttttctt
    53161 ttgttttagg gttttctttt ttgtggtctt ggattgcttc tttcgtaatc ttttcccgat
    53221 tttttttaga tgaatctaag cgttctgcgt atactttttc ttttggctta tcattcattg
    53281 gaaaggacct ccctacatta tttcattcta cctctagttt atcatctgtt aaggactagt
    53341 ggaaacgttt tgcctaactt ataagctgat ttttttagca gataagcttg caagtaaaaa
    53401 acaaacaagc tacaattgat ttattgaaat tgttcctgta gctcagtagg atagagcaat
    53461 cgcctcctaa gcgataggtc gctggttcaa ttccagtcag gaacgtataa ataacccctg
    53521 tatcctttgc ggtacagggg tttgttttgt atttgtgcca aaaatgtgcc aaactagtta
    53581 tcatttttat ataaactatc gaacacagta ttgatttttt ctttatcctg atcttcaagc
    53641 tctcgaacaa tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt
    53701 ttcgatacgc taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt
    53761 agtccatgca aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat
    53821 ttgctgacgg cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaa
//
LOCUS       DAYYNQ010000007        49323 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000007 DAYYNQ010000000
VERSION     DAYYNQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.007"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(479..2371)
                     /locus_tag="ACHBJ1_000409"
     CDS             complement(479..2371)
                     /locus_tag="ACHBJ1_000409"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429382.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2368..4095)
                     /locus_tag="ACHBJ1_000410"
     CDS             complement(2368..4095)
                     /locus_tag="ACHBJ1_000410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429383.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4111..4761)
                     /locus_tag="ACHBJ1_000411"
     CDS             complement(4111..4761)
                     /locus_tag="ACHBJ1_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM9429384.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4965..5261
                     /locus_tag="ACHBJ1_000412"
     CDS             4965..5261
                     /locus_tag="ACHBJ1_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9429385.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5453..6073
                     /locus_tag="ACHBJ1_000413"
     CDS             5453..6073
                     /locus_tag="ACHBJ1_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9429386.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6165..7331)
                     /locus_tag="ACHBJ1_000414"
     CDS             complement(6165..7331)
                     /locus_tag="ACHBJ1_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9429387.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7343..7696)
                     /locus_tag="ACHBJ1_000415"
     CDS             complement(7343..7696)
                     /locus_tag="ACHBJ1_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9429388.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7824..8846
                     /locus_tag="ACHBJ1_000416"
     CDS             7824..8846
                     /locus_tag="ACHBJ1_000416"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM9429389.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAVNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8865..9026
                     /locus_tag="ACHBJ1_000417"
     CDS             8865..9026
                     /locus_tag="ACHBJ1_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429390.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9285..9719)
                     /locus_tag="ACHBJ1_000418"
     CDS             complement(9285..9719)
                     /locus_tag="ACHBJ1_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429391.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9829..11688)
                     /locus_tag="ACHBJ1_000419"
     CDS             complement(9829..11688)
                     /locus_tag="ACHBJ1_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9429392.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11689..11829
                     /locus_tag="ACHBJ1_000420"
     CDS             11689..11829
                     /locus_tag="ACHBJ1_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM9429393.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11806..12966)
                     /locus_tag="ACHBJ1_000421"
     CDS             complement(11806..12966)
                     /locus_tag="ACHBJ1_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9429394.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13273..14697)
                     /gene="araA"
                     /locus_tag="ACHBJ1_000422"
     CDS             complement(13273..14697)
                     /gene="araA"
                     /locus_tag="ACHBJ1_000422"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM9429395.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14713..15411)
                     /locus_tag="ACHBJ1_000423"
     CDS             complement(14713..15411)
                     /locus_tag="ACHBJ1_000423"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9429396.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15368..17029)
                     /locus_tag="ACHBJ1_000424"
     CDS             complement(15368..17029)
                     /locus_tag="ACHBJ1_000424"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM9429397.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17247..18647
                     /locus_tag="ACHBJ1_000425"
     CDS             17247..18647
                     /locus_tag="ACHBJ1_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM9429398.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18899..19978)
                     /locus_tag="ACHBJ1_000426"
     CDS             complement(18899..19978)
                     /locus_tag="ACHBJ1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9429399.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20064..21023)
                     /locus_tag="ACHBJ1_000427"
     CDS             complement(20064..21023)
                     /locus_tag="ACHBJ1_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM9429400.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21035..22546)
                     /locus_tag="ACHBJ1_000428"
     CDS             complement(21035..22546)
                     /locus_tag="ACHBJ1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM9429401.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22568..23131)
                     /locus_tag="ACHBJ1_000429"
     CDS             complement(22568..23131)
                     /locus_tag="ACHBJ1_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9429402.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23137..24000)
                     /locus_tag="ACHBJ1_000430"
     CDS             complement(23137..24000)
                     /locus_tag="ACHBJ1_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429403.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24014..24910)
                     /locus_tag="ACHBJ1_000431"
     CDS             complement(24014..24910)
                     /locus_tag="ACHBJ1_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429404.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25085..26374)
                     /locus_tag="ACHBJ1_000432"
     CDS             complement(25085..26374)
                     /locus_tag="ACHBJ1_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9429405.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26346..27863)
                     /locus_tag="ACHBJ1_000433"
     CDS             complement(26346..27863)
                     /locus_tag="ACHBJ1_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM9429406.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28061..28951
                     /locus_tag="ACHBJ1_000434"
     CDS             28061..28951
                     /locus_tag="ACHBJ1_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFM9429407.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29024..29848)
                     /gene="nadE"
                     /locus_tag="ACHBJ1_000435"
     CDS             complement(29024..29848)
                     /gene="nadE"
                     /locus_tag="ACHBJ1_000435"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM9429408.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29860..31332)
                     /locus_tag="ACHBJ1_000436"
     CDS             complement(29860..31332)
                     /locus_tag="ACHBJ1_000436"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM9429409.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31969..32331)
                     /locus_tag="ACHBJ1_000437"
     CDS             complement(31969..32331)
                     /locus_tag="ACHBJ1_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9429410.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32554..33306
                     /locus_tag="ACHBJ1_000438"
     CDS             32554..33306
                     /locus_tag="ACHBJ1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9429411.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33446..34369)
                     /locus_tag="ACHBJ1_000439"
     CDS             complement(33446..34369)
                     /locus_tag="ACHBJ1_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9429412.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34384..34656)
                     /locus_tag="ACHBJ1_000440"
     CDS             complement(34384..34656)
                     /locus_tag="ACHBJ1_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429413.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(35000..36187)
                     /locus_tag="ACHBJ1_000441"
     CDS             complement(35000..36187)
                     /locus_tag="ACHBJ1_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM9429414.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(36600..38225)
                     /gene="groL"
                     /locus_tag="ACHBJ1_000442"
     CDS             complement(36600..38225)
                     /gene="groL"
                     /locus_tag="ACHBJ1_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM9429415.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(38276..38560)
                     /gene="groES"
                     /locus_tag="ACHBJ1_000443"
     CDS             complement(38276..38560)
                     /gene="groES"
                     /locus_tag="ACHBJ1_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM9429416.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            38785..39447
                     /locus_tag="ACHBJ1_000444"
     CDS             38785..39447
                     /locus_tag="ACHBJ1_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9429417.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(39523..40815)
                     /locus_tag="ACHBJ1_000445"
     CDS             complement(39523..40815)
                     /locus_tag="ACHBJ1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9429418.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            40985..41614
                     /locus_tag="ACHBJ1_000446"
     CDS             40985..41614
                     /locus_tag="ACHBJ1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9429419.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(41717..42526)
                     /locus_tag="ACHBJ1_000447"
     CDS             complement(41717..42526)
                     /locus_tag="ACHBJ1_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9429420.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(42581..43450)
                     /locus_tag="ACHBJ1_000448"
     CDS             complement(42581..43450)
                     /locus_tag="ACHBJ1_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM9429421.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(43451..44767)
                     /gene="yycH"
                     /locus_tag="ACHBJ1_000449"
     CDS             complement(43451..44767)
                     /gene="yycH"
                     /locus_tag="ACHBJ1_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM9429422.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(44764..46599)
                     /gene="walK"
                     /locus_tag="ACHBJ1_000450"
     CDS             complement(44764..46599)
                     /gene="walK"
                     /locus_tag="ACHBJ1_000450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM9429423.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(46604..47308)
                     /gene="yycF"
                     /locus_tag="ACHBJ1_000451"
     CDS             complement(46604..47308)
                     /gene="yycF"
                     /locus_tag="ACHBJ1_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM9429424.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(47498..48358)
                     /locus_tag="ACHBJ1_000452"
     CDS             complement(47498..48358)
                     /locus_tag="ACHBJ1_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9429425.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(48345..48914)
                     /locus_tag="ACHBJ1_000453"
     CDS             complement(48345..48914)
                     /locus_tag="ACHBJ1_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM9429426.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
BASE COUNT        14360 a        10358 c         8507 g        16098 t
ORIGIN      
        1 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaaa
       61 agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcta catttttgca
      121 taaatttggc ttaataccga gaaactggct tttgaacacc gtttaccaag aggttgtgag
      181 cctgccgttt aactccgagt cacaaggagc aatttcaaaa aacagcttct caggtttgtt
      241 gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagaggttgt
      301 aaaatcagcg gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt
      361 ttgccgtttt ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa
      421 gggactgtga cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct
      481 atgaagcttc ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt
      541 ccatcaatgt atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt
      601 tgtctgcatc gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca
      661 ataggcggtt catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg
      721 cttcatcaag gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct
      781 gacgctgccc ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg
      841 gcaatttacg aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt
      901 ctgttgcatc gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag
      961 tatcttgcag taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat
     1021 ctacaccatc gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga
     1081 tcagtgtcgt cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga
     1141 catccaagtt aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt
     1201 caaaacgaac tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca
     1261 tctcttcttc atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag
     1321 tgttcatcaa acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga
     1381 acgcttgaac atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag
     1441 cgacgaacac atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa
     1501 taaattgagc tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct
     1561 ggtctttttc agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta
     1621 cttggttatt caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg
     1681 gcgctacgat catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca
     1741 attgccagct gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta
     1801 caaattgagt caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc
     1861 gagacatgat atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt
     1921 tggcttccaa atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa
     1981 attgctgtaa aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga
     2041 tctgcgcgat tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct
     2101 gttgagcgcc agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg
     2161 gtgtttggat ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa
     2221 ccatacgttt agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct
     2281 tttcaccttt tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc
     2341 catgagaggg acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg
     2401 aacgatttct tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac
     2461 cagttttcct tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg
     2521 agcaactaaa acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc
     2581 atccgttttg aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt
     2641 gactaatgcc cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc
     2701 ttgttccaca tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc
     2761 gatttctaat gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt
     2821 gtctcggatc gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg
     2881 caaattatat tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc
     2941 gatgtcatat aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc
     3001 tatgattgcg acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt
     3061 ttctgctcca tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc
     3121 aaaagaaact gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc
     3181 attaatacgg tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat
     3241 cataaagctg atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc
     3301 caaagtcata tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct
     3361 catgatcaat gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt
     3421 atttgtataa tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa
     3481 tgctcgaatc actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt
     3541 tttcttttgc atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat
     3601 aatcattact gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc
     3661 aagtatgctc gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg
     3721 tgtaaccatt tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc
     3781 atatttatca aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa
     3841 ttgcttaccc agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa
     3901 gatactgatc aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc
     3961 tccttgcgct acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc
     4021 cagtacttgg atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt
     4081 tacgattttt agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga
     4141 aagtttcaaa ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa
     4201 taagcgatac gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa
     4261 ttccaattca tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc
     4321 ttttgcatct tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg
     4381 aggagcgact tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga
     4441 agcattctcg ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt
     4501 gaatagatca ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct
     4561 cattgtctca aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact
     4621 gcctctaggg atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac
     4681 tctagaaaac tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg
     4741 gaaaaatgtc tgtgtcggca tatggattct cctttctttc aagatgacac attgtcatat
     4801 gacgctatgt cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc
     4861 attatcatcc gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga
     4921 gttgcccatt aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta
     4981 gtgtccaaga cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa
     5041 ataccttaga ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct
     5101 tgccaagaca attaaaaaat ctagatccaa agaaacttta ttatattcta agctatacag
     5161 gcaggcgatc agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata
     5221 tcattggtgg gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca
     5281 aaagaggctg tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt
     5341 atttattctt caaccttatc aatatgactt gatagccttt tctttttcga gtaaactgat
     5401 tttgttagtc atagttataa caatcatagc aataaaagga gaaggtaagc atatggcatc
     5461 tgttttagta gtcaaaggtc atcccctaac tgcagaagaa tcgcgcacag ttaaggcatt
     5521 gacgtctttt ttaacaagtt ataaagaaaa tcatccagat gatgaagtga ctgttttgga
     5581 actttatcgc gatgatattc cggaaatcga tgaagaatta ttatcaggtt gggaagcatt
     5641 acgtgctggt gcagaattca cttccttatc agaaagtcaa caacaaaaaa ttgctcgttt
     5701 caatgaattg actgatcagt tccttgctgc tgataaaatc gttatcgcaa acgccttatg
     5761 gaatctaaat atcccaacaa aattaaaagc ttggttcgat accgtcaatg tagctggaaa
     5821 aacgttccgc tatacagaaa acgggccaga aggattagtg acaggtaaaa aagcactgca
     5881 catccaatct aatggcggtg tatacaacgg acaagacttt gcctctcaat atgtaaaagg
     5941 tattttaaac tttgtaggca tcgatcaagt agatcagcta ttcatcgaag gaatcgatta
     6001 tgaccctgac cgtgcagatg agttgatgca aaatgcatta gataaagctg ctgctttagg
     6061 taaatcattt taaaaattga gataaatgca tacaaaagag acagaggaaa aaatcttcaa
     6121 agcagcattt gtgccttgga tattttccca tctgtctctt tttgttattc tttctgcttt
     6181 ttcaacggat tttcttcata aagagaagag attgttgtgt accattcaaa aacatgagta
     6241 atcactactt tagctgctgc ataaacagga atccccaaga tcacaccgat gattccgaac
     6301 aatttcccag aagtcagcag aactaataaa atcgttactg gatggattgc taactgattc
     6361 cctagtacta atggagagat caagcgtcct tctattgtct gctctactgc aaaaacgatc
     6421 agtactttga tgatcatgaa tggacctgct acaatggcta aaaagatagc cggaatcatc
     6481 gctaaaaatg atcctaagta agggatcagg tttaaaaaac ccgcaataat ccccaacgtg
     6541 acagcatact ctaaaccaat gaccgcaaaa ccaatcataa acatgattgc cacagcaaaa
     6601 gccacagtta gttgtcctct tatataagaa gaaacttgac tattcatttc actcagcaca
     6661 ttcaacgtcg gtttttgcat gcgtgtcggt aaaaatttga caaaataagg tgctaattgt
     6721 ttaccgtctt tcaatagata aaataagata aacggcatcg tcagcagagc cactaaaata
     6781 gtcgctaccg cgccgacaaa acttccaaga ctctgaactg tactcttaga tatatcttgg
     6841 atcatatcgc ctgccgaatt catgaactta tcacctgctg tttccaactg ttcgcgaaat
     6901 tggttgaaca gcgggtcccg caaaatctcg gttaatttat tatcaatcgt atctacatat
     6961 tgagggaagt tactgatgaa gctcactgtt tgttcctgaa tcttagggat gatcactacg
     7021 ctcccccatg cgattagtgc aacaaccaaa acaaacagcc caatgatact gtaaagcctt
     7081 ggtacttttc gcttttcaaa gtaatcaact accggattca tcaagtaata caagattcct
     7141 gctaaaatga taggcagacc tacgattgct aaaaattgcc agattggtgc aaacaaatat
     7201 gaaacttttg taaataaaaa gacgatcagc aatattaaca atacaacaag taaggctgtg
     7261 actacttgat tatttaaaaa ccagcgccaa aaccacgata ccggtctgtt cttcttattt
     7321 tttccttcca taattcctcc tgttagttat aaaaaatttg gcttcccaaa ccaatagcag
     7381 gcagtctatg cggatgaaga tagattccat ttgcaatttg tccttcttct ggacaagcat
     7441 caaaaatcaa tgatacgtga gctactgtct ttaaattccc attggccatc ggaccgacat
     7501 aatcgatcgt atatacctga tcatcaaaag caattgttcc gcctgctttc aaagaaaaag
     7561 gttcgtcttt tgatacttct tgaatcaccg aatattttcg taaggcagct gttgctgttt
     7621 tatcgaacaa aatcagcatg ggttcttcct tgtttattgc ttctgtccca atttctgtaa
     7681 tctttgcctg aatcattgca ctctctcctt taattgcttt caaaaacagt ataacacatt
     7741 ctcattcttt agccgtgtgg aataattatg tgatagaata aaaacgaaaa ctatttcatt
     7801 acaattagga ggaatcagct catatgatcg aaaagataat tttaggaacc tatacgcgtc
     7861 gcgaaagtaa aggaatctat acgatcgacc ttgatacaga aaaagaagaa ttagccaacc
     7921 tttcattagc tgttgaagaa aatagcccta cctatgtagc aaaaagtaaa gctgtcaatc
     7981 tttacacagt tactagtgtg gatggattag gtggtgccgg tgcgtacgat aaggattttc
     8041 attttttgaa tgcggtaaca gagtctggcg ctcctttgtg ttatgtagcg gttgatgaag
     8101 cacgtcaatt actatacggg gcgaactatc ataaaggtga agtaaatgtc taccatatat
     8161 taggagatgg cgaactgaaa gcagcagatt caatattcca tcaagaagcg acaggtcctc
     8221 ataaaaacca agatcatgcg catgtccact atacggatct gactcctgac caacgattag
     8281 tcgtatgtga tcttggtaca gatcgtgtgt atacttatga tgtgtccgaa aacggcaaat
     8341 tgaatcttgt aactaccttc actgcagagc caggaacagg ccctcgccat cttgtctttc
     8401 atccaaatgg acaattcgct tatttatttg gtgaattaga cagcacagtc cgcgtgcttt
     8461 cttataataa agaaacaggt gcttttgaag aattacagaa aatatcgacc ttgccagaag
     8521 aattcgacgg tgaaaatgga ggggcagcta tccgcatttc aaatgatggc aagttccttt
     8581 atgcttctaa ccgaggacat aactcgattg ctgtattcgc agtttttgaa gatggttccc
     8641 acattcaaaa caagcaaatc atctcaacag aaggggattt tccccgcgat ttcgcactag
     8701 atccaagcaa taattttgtc gtgtgtgcca atcagaacac cgataatctg acattatatc
     8761 gccgtcatcc tgaaacagga ttactggaat tactgaaaaa agatgtctat gtaccagaat
     8821 gtgtctgtgt ttattttgat cgttagtaag aaaaggagaa tgaaatgaat cagcaagacg
     8881 tattagacag actacgggaa gaactaaata ttcctttctt tgatgggaaa atcgaagata
     8941 aagagtatag cgaagaggaa tatcagaaac tcaaagccga tttacagaac tactttgaac
     9001 aatatgtaca gaatgtagaa aactaatcag ctttccatac tatctgaaaa gaacatcagg
     9061 acaagacttt tccgtctatc ctgatgttct tttctacaaa taaaaaaccg ccgagatggt
     9121 tagactcgac ggaaaaggag tatttactcg attttgagta agtacaaggt tgttgttggt
     9181 tataagtatg tcattatcat aaaggatttt gatcactcta tcataaaggt aatattacat
     9241 ttatattaga taactctagt ttttattgtt atcaaagatt tcttttattt tttcaacaac
     9301 aaattagtca ttttttggat ggcagctgtc aattggtctg tcgcttggtc ttgttttgct
     9361 tcagatgtat tgaatagatc ttcatctacc cctgattcaa gatcgatact ttggatcatt
     9421 tcttcacagc ctgctacata tgaaagataa gctttttcaa aatttttatg aatgcccatt
     9481 gcttttgctg gtggacgcaa agtactgact ttcataagca ttgcccgata tttttttgtt
     9541 ccttcttcaa ataacaggat gatctcttca agagtcgatt tgtccagttc acttgttttt
     9601 ccttcatcaa ttgcgtttct gatcacttca tatttcggat gcattccttc tccgattgct
     9661 tctgtttcct tgacgatttg atcgatggac tgcacataat agccaatatt cggtaacatg
     9721 ttcgttttcc tactttctcc attgttgtta ttatttattc tacttgtttt tatcctcaaa
     9781 tgacaagtac ttgaaaaaat ttaatggttt ctatacagct cgccattctt attcgtgctc
     9841 aaagaaacca tctgctaaac tatattgttt ccctgcgaca atgtcatatt ggatcaattg
     9901 atattcgtcg taaatctctt gttgcgagcg attgaattgc gcttctttat accactgtcc
     9961 gttttgatag tacagttctc gttcaaacgc cggaagttcc tgttctaatg ataaaagcag
    10021 ctgataaaag ccatttgttg tctgattcgt ctgttccatt agattcgctg caaaataaaa
    10081 cggactggtg attgcatcgt gagtcgtttg cttttctaat tctcctttac tgtcgaacat
    10141 aagaaattgt gtttcatgca atgtttgttt gtcatttttt gcttggatcg tatctgaata
    10201 gatccctgga agatgatccc cccaaaaaac aactaaagtc cttctagaaa gattcttcaa
    10261 ttcttccgtg aactgcttca gtgccgtgct gctataggaa atatcttgca gataattctc
    10321 taagtcaagt gtcccacttc catctgaagt ttttgcagga tagtctagat tatcatactt
    10381 tccatcgtag ggcatatgtg tctgcatcgt gactaaatga atgaactgag gtgttttttc
    10441 ttccttgaga agtgtcatta cttctttgta agctgactca tcagaaatat atggattatt
    10501 ctctatcgta tccgtatagg tcatcgtacg ctcgctgatg aactggtcaa atcctaatgt
    10561 ctgataaaca tcctctcttt tatacatcga agtattgtat ggatggatcg ctgtcgtctg
    10621 gtatgattgt gcgtttaacg ctgaaacgat agaaggaagc tgatccattt ttggtacaag
    10681 catcgtgtaa ggggtggtga gttgcgcatt aaataacgcc atagaaaaac cagtcaatgc
    10741 ttcaaattcg atatttgctg ttccaccgcc atagttttga gaaagcatat tgccactata
    10801 tgtttgatca gctacttcat aataatctgc caatggctca ccagatactt ctacgccgtt
    10861 taatcgacta ggatctgaga aactttcact catcacgaac acgatgttcg gcgactcttc
    10921 ctctacagtt ttattttttt catcggctag cttctggtat ttttcagtga tttctttgat
    10981 cttttctttc gaatatcctt caggttcatc cattggttct accttcagat tgtacaaaaa
    11041 cccaccgata aaaccagtgt tatagtagtt cattttctgg ctgtaaggta tccatagtgc
    11101 cgttttatta tatgcttttc gcaacaaatt atcgggattg ttgaaattac tgatataccc
    11161 tagcagtcca attgccacaa gcagtgtcgt taatcggatc aattggaatc gccttccttt
    11221 tttcaggctt cgaaacatgt accagcaaaa caatcctatc acggttcctg cggcagctaa
    11281 gatcactgta aacagcatag tgccggtcat atctttcagc aatccgattt cagtgatcat
    11341 cttcaaatcg tctggataaa tcggttcttg ccgataactc attttcatat aatttgcaaa
    11401 acctaataca ccgatggcta cgacatacgt aagggaacct aaagggacag atcctgctag
    11461 tgcaatcaga aaaataagaa taatcagcaa gacgagacag gctaagaaga atttttccgt
    11521 atgccaagaa aaagcaaatt tcaacgcaag gtctacggat agatcatttt gacaccactg
    11581 caaaaataaa ttactaaaaa caacaagaaa aaagatactg atgattgctg ctgttccttt
    11641 taaaatcttt ttctgtttgg tcgaaagtct gcttatatat cttttcaaat gcttgctcct
    11701 tcatttctat caataaaaga atgttgcttc tcttatttta gttgtacgaa attcgaaagt
    11761 aaagaacgga aagaagaatt aatgaaatcc aaatattctc tttctttatg gcctttctgg
    11821 tttctttaat aagcggacct ttcgtactcc agaaattttt tctagctgtt gtttcacttg
    11881 attgagctgt tcaaaatcag tactgtctat gtcaatcaat atgtagacat gctcattctt
    11941 atggttacgc gatatatctg cgatcgctag ctcgttctca ttcagcagag aaaatatctc
    12001 tgctaatata ttggttttct tttgataaaa tatagtgaat cgaaacggcg cttggaaaaa
    12061 cattcgtgcg gatggataat tgaccgcttc tctaaccgta ccgaacaaaa gataatcttt
    12121 taaggcttca actgccaaac gtccgctgtc tgccaaagct tcctgagtcg atccccctag
    12181 atgaggcaac ataagtatac ggtcattttc taagaactct tcttctggaa aatcagtcaa
    12241 gtatttagcc agatgttgtt tttccaatgc ttgcataaca gcatatttat caacaatttc
    12301 acttcttcct acattgataa gtacagcatc ttttttcatt ttttctatat tcgcctgatc
    12361 gatcaatcct tttgtatctt ctgtcaacgg cagcatgact acgatgtaat ccgaggtaga
    12421 aagaactgtt tctaaatctg cttgccgaac ataatccaac tgggcatcgc ggatagaata
    12481 acccaagaca tccatcccta agctatagca acttagcgct acttttgtgc ctatcgctcc
    12541 aagaccaaga agaccgatgg ttttgccttg caattctcgt cccacataag cacttctttt
    12601 gttttctgcc tgttctaaaa tattaggacc tgttagtgtt tgtaccatac gacttgcttc
    12661 gattatcgga cgggaagata agagcaaaca gcacaaaaca agctctttta ctgcattagc
    12721 atttacacct ggtgtattca tgacgatcgt cccgttttca gaagcttttt caacgttgat
    12781 cgtgttcacc ccaacaccag ctcttgaaat tgctagcaaa tctggtgtaa gccattcatc
    12841 tttcactttc gtactacgaa taactattcc ttcggctggc aaatcatcta ttattttgaa
    12901 atattcccct tgtaactttt ctatctcttg ttctggaata gcatcaatca aatgaacgtg
    12961 atacataaaa ccccctcctt actaacaagt atctcgaaat ttctgcaatt agacaactgt
    13021 ttaccatttg acttcctaga agcaaaaaga gcagttagag cagagtaggc tgagtgataa
    13081 aaataaggct atgacaagtt actgccataa gccttatttt cgagagcaaa atattgaggg
    13141 tgagatatct tgataaatgg tgttccgtta gctgacattg ataataagac taaatttcaa
    13201 aaaagttgaa aagcaaattt cgtcaatctc ttcttatttc ttaaaactaa gtggcttttt
    13261 cacaacctct ttttatctaa taaccactgt ttccaactca actaattttg cccacgtttc
    13321 aatttgatct gttgttagat ttagcgagac aacggtatga tgaccgccac ctgcctgaat
    13381 ccatgaatgg acaccttcat ggaaattcgg tttagttttc cataacacac gtgcaacagg
    13441 taaattaggt gcttcagtcg ttggttcaaa ggcttctact tcattgatca gcagtttata
    13501 atgcgtaccg aaatctgcca tcgatacgac taccccttct cctgctttcc catcaaatac
    13561 aagacgcgcc ggatcttctc tatctcccat tgataaagga gaaacaacga ttcttggttt
    13621 cgtcgcagca agcgtcggat ctacttccat catatgggat tccaagattg cttcttggcc
    13681 actagctagc tcgtaagtat aatcttccat gaatcctgta tctttccctc gagccatgat
    13741 tttcagcaac cgatcaatcg ctgcagtttt ccagtcgcct tccccagcaa aaccgtatcc
    13801 ttctgccatc aaacgttgta cagctagtcc aggtagttgt ttcattccat acaggtcttc
    13861 aaaattagtc gtaaaagcgt tatagcctcc ttcttctaag aaacggcgaa taccgatttc
    13921 ctgctgagct tgtactttta catgttcctc ccatgtcttt tcttcataat caccatagtc
    13981 gaaggtatac aattccttgt actcttcaaa taattgatcg acttcttcgt ctgatacgcg
    14041 atcgattact tgtacaagat ctccgatacc aaaataatca acagtccaac caaattgaat
    14101 ctgtgcttcc actttgtctc cttctgtgac acccacgttt ctcatgttgt caccaaaacg
    14161 agccactttt attccaaaac tttcattata ggctactgca acatccatcc aatcagcaat
    14221 ctctttttgg atctcaggtc gtttccagta gccaacgacg attttatttt gtttatttaa
    14281 tcgagcattg atgaacccat attcgcggtc accatgagcg gattgattta aattcatgaa
    14341 atccatatcg atcgttttcc aaggaatgct ttcgttatat tgcgtagcta aatgaagcaa
    14401 tggtttttgt agaagctttg tcccgcggat ccacattttt gctggtgaga aagtatgcat
    14461 ccaagtgatc acacctgcta cttcttctcg ataattgacc tctttcatga tttttgtgat
    14521 cgtatcagca gtaactgcta attcttgaag gcggattgga taaggcaatt ggccattttc
    14581 attcagttca tcgaccattt cttgtgcatg tttctttact tctcgcagtg cttcttcccc
    14641 atataaatgc tgtgatccta caacaaacca aaattctttt tctccaatat tcaacataat
    14701 tagccactcc ttttatttgt tttgtccgta atacgcattt tttccgtgtt ttcgtaaata
    14761 atgtttatct aaaatccgtt gaggcagctc ttctgaaaat gaattgagct gtctggtaaa
    14821 taaattcatc ttgcatactt catctagtac gactgcgttc attaccgcac tttgcgcatc
    14881 tttcccccaa gtaaatggac cgtgtccgtg taaaagtacc cctggaattg ctaagggatc
    14941 aattttgcgt tctttgaatg tttctatgat aacttttcct gtttcatatt cgtacccgct
    15001 gtcgatttcc tgttgtgtca aaaatcgagc acaaggtacc gatccataaa aagtatctgc
    15061 atgcgtcgtc cccatcgcag gaacatccaa tcctgcttgt gcccagatcg tcgcccatgt
    15121 cgagtgagta tggacgattc ctccgatttg aggaaatgaa cgatataaaa cagcatgagt
    15181 aggcgtatcc gaagagggat tgagtttacc ttcaatgata tgattgtcca agtccacaac
    15241 gaccatgtcg tctgctgtca tctcttcata actgactccg ctaggtttga taacgaaata
    15301 tcgtgtttca ggatcaaaag cgctcacatt tccccatgta tatttgacga gtccgtgttt
    15361 aggcaagtca agatttgctt ggtatacttc ttctttcagt tgttccagca ttttctatca
    15421 ctcctttttc ttgcaattga tgaatcgctg ttttttcaat gtcgatcccc tcacggtaac
    15481 gtctcataaa tatctgatag cctgctattt cttctgctga aggtgtgatg gtgattcctt
    15541 ggtcttgtcc aaacacgtct tctgccaaga agttttctaa agacttccca tgtcgatcca
    15601 tcatataggc tgctaataac gcgattcccc acgctccacc ttctccggct gtgtccatca
    15661 ctgtaactgg tgcctccata gcagatgcta gaatccgctg tccaacttct ggtgttttaa
    15721 aaatcccccc atgccccact aaacgatcaa tttggacatt ctcagatttc aatatatcca
    15781 ttccgattct cattgcacca aaagcactgg ctaaatggat ccgcatgaaa ttcgctaaat
    15841 caaaacggct acctggtgta cgtacaaata atggccttcc ttcatttact cctgtaatgt
    15901 tttcaccgga aaaataacca taagaaagta atcccccgca atccgtatcg ccttctagcg
    15961 ctttgttgaa taataccgtg aataatttat ctctgcttat ctccataccc aaagaattgg
    16021 taaattcttc aaaaatactg acccaagcat tgatatccga agagcagtta ttcgtatgaa
    16081 ccatgcctac tagttctcct gaaggtgtag tgaccatgtc gatttccgga tggacatttt
    16141 ctagttcttt ttctaatacg atcatagcga aggcggaagt accagccgaa atgttccctg
    16201 tccctttggc tacactgttc gtagcgacca taccggttcc tgcatcccct tctggcggac
    16261 aaaccgggat tcctggtctc aaatttcccg tagggtcgat cagataagcc cctgattcgc
    16321 ttaacttgcc agcttcttct cctgctacta gaactttagg caaaatgtga tccaagtcaa
    16381 tcgctacgtg cgctttttcg attaactgac caaaaatatt tttcatttct tgatggtagg
    16441 ttttcgttga aggatcgata ggaaacatgc ctgatgcatc tcctacgcct agtactttct
    16501 caccagtaag ctgccaatgg acataacctg caagcgttgt caaaaaatta atttctttca
    16561 catgttcttc tttattcaaa atagattgat acaaatgagc gatactccat ctctgcggaa
    16621 tattgtattg aaatttttcc gttaatattc gttctgcttc ttcagtgatt gtatttcgcc
    16681 aagtacgaaa tggtactagt aactgttcct tgctatcaaa tgctagataa ccgtgcatca
    16741 ttgcactgaa cccaatcgac ccgattgtcg taagattctc gccatagatt tcgaatactt
    16801 ctgctgccat ttttcggtaa ctgacgcgca gtcctttcca aatatcttct aatgaatagg
    16861 tccagatacc attttctagt ttgttttccc actcataact accagcggca ataggagtaa
    16921 aagatgaatc aatcaataca gctttgatcc tagttgatcc aaactcaatc cctaaagatg
    16981 tacgaccatc tatgacatct aacatacttt gcgcatttac ttccttcata atgatttaac
    17041 tccctccttc tcgtcaaacc tgatcaaacc gtctttttag tagcgcttac aatgattatt
    17101 gtatactttt gtacgtacat gtcaagcagt tatttacaag aatacaataa aaaacacttg
    17161 ttttgtacgt acataagtta taaaatgaaa ttgcttacaa aatctgatca accgccttct
    17221 cgaaattcta gaaggagaga atacttatga aaaaagaaaa aaaagtttcc tcatcgttta
    17281 tttatttttt tggcgcattt ggcggcattc tatttggata tgatattggt gttatgaccg
    17341 gcgccttacc ttttttacag catgattggg gtttggcagg taaagcttct ttgataggat
    17401 ggatcacgtc ttcggtcatg ttaggtgcga tccttggcgg ctctttatct ggtcttcttt
    17461 ctgataagct tggacgacga aaaatgattc ttctttctgc acttattttt atggctggtt
    17521 ctgtcctttc tgcatctgcc cctcacaatg gttcttattt tctgattgca gcaaggattc
    17581 ttctaggttt agctgttggc gctgcatctg ccctagttcc tgcttatatg tctgaaatgg
    17641 ctcctgctag actaagggga agattgtcag ggatcaatca agtgatgatt gcttctggca
    17701 tgctcctttc ctatgttgca gattatttgc taaaagggtt gcctgaaacg atggcttggc
    17761 gcgtgatgtt agggctcgct gctgtccccg cgctgatttt atttttcggt gttttagctc
    17821 taccagagtc accgcgtttt cttatgcaat ctggccgctt ggaagaggca aaaagagtat
    17881 tgaactatat tcggacacca aaagaagctg aacaggaatt cgaacaaatc cagctgaatg
    17941 tcaaacaaga aaagacaacc ggaacttctt ggcatacttt gtttttagaa aaatatcgtt
    18001 ccttagtcat tgccggtatt ggtgtagcag tattccagca atttcaagga gctaatgcta
    18061 ttttttacta tattccgctg atcgtagaaa aagcgactgg gcatgcagcg agcgatgcat
    18121 tgatgtggcc gatcatccaa ggaatcattt tagttgctgg ttctttgctc tttttggtta
    18181 ttgcagacaa gttcaatcgg cgaactttat tgaaaatagg cggtagcgtg atgggacttt
    18241 ctttcatcct accagctgtt ttaggaactg tactggatgc tcatacaaat tcgttgttga
    18301 ttctcttgtt tttatgtatt tatgtcgcat tttattcttg cacatgggct ccactgacat
    18361 gggtaatcgt aggcgaaata ttcccactag ctgtccgcgg gcgagcatct ggcctagcct
    18421 cttcttttaa ttggattggt tctttcctag ttggcttgct ttttcctgtg atgactgctt
    18481 ctatgtctca agaaatcgtt ttcggcattt ttgggatcat ctgcttttta ggggttttat
    18541 ttattcaaga gatcgtacca gaaactcgtg ggaaatcttt agaggaaatc gaacaaagtg
    18601 catctaaaaa aacttatcct aaacgtattt ctatccatca ttcttaaaga ggttgtgaca
    18661 gaagtggtca gcttcaagaa ataagaagga accgcttagt ttccagtcac aaggaacaat
    18721 ttcaaaaaac agcttctcat gtttgatgaa tattgtgact tgtgaccagt tggtaggaga
    18781 acaccgttta gtggagaggt ggtgaaagaa acgttcagcc ttgagcgatg acaaaagctc
    18841 tcacaaacat tgcgacgagt aaccgtagga gcaataagag gcggtaaaaa cccgtctctt
    18901 atttttcgtt ttctttcact gtttcccgtt caactagtac aggttcatag agaatatttg
    18961 gctgtgcctg tcctttttca atcgttgtga cgatccaccg tgcggcatct tgtcccaact
    19021 tttcttttgg atgcgtcaat gttgtcaatt ttttattccc taaatggctt aaagaagaat
    19081 cgtctgttcc tacgatcgat aggtcatctg gaatatgata gccattttct gtcaacttat
    19141 ctagcattaa actagctatt tgatcgttat agcaaatgat gccatctaaa gcttgttctt
    19201 ctttcaaccg ttgtactgtt ttttcagcga ttgtttctcg tgtttccgtg gtgaaagtaa
    19261 tgatatcttc cgtcaaaaaa cgcacattat actgttcaca cgcgctgata aaacctttca
    19321 tcctgtattt tccttgaaga tcgtcgattt ttgtgatgaa caacagacgc tgatgtcctt
    19381 tttttatcaa atattctgct gccataaaac cgactttcac atcatctaaa caaatataag
    19441 aggcatttaa ctcttcataa gcagcattga tcatcaccat cggaatgcct tgttctctca
    19501 aagcaacata aagtgctaag tttgggttat actgattact tttcgtaggc tcaacgatca
    19561 aaccatctac cccataagca atcattttct tcaaacactc tttctccaat tcgtgatcgt
    19621 tattcgtact ggctaaaagc aatgaatacc cagattctcg taattcattt tcgattcctc
    19681 ggataatcga cggaaaaata taatcagaga gatatgtggt gatgacaccg atcgtttttg
    19741 ttctgttcaa tggtgttttt tctttcttat agctgtcatc tacatatgtg cccgaccctt
    19801 tttctttacg cagatagccc tcatttacca acacagcgat tgcttgccga atcgtatgtc
    19861 tgctgacgtc atatatttct tgcagcttga attctgaggg aatggttgta tttacttcgt
    19921 attcctcacg aaggatcttt tgttttatct cttctgcaat atcttgatat tttgccattt
    19981 gccttgcccc tttttatttg tacgtacata tatcataacg agtctgcaag gtttattcaa
    20041 gcaaaaatca aggtttcttg tgctcataag gcatacggaa caggttcacc aaattctgga
    20101 aatccttgct cattccacgt aaagcattgc gcgttcgcgt gccgattagg attgtctaac
    20161 ggatcccctt tttggttctt ctctggtcga gcatgataga tcaaaatatc aaagcacccg
    20221 tcttctgaaa ccgtaaacga gttgtgacca ggaccaaatt gcttattttt tttagaagaa
    20281 acgaatactg gctccgataa tttatgccat gattctttta ataaaagatt cttgtgctca
    20341 tcagcccaca gcaggcccat ggcatagttt tcatctgttg cactaccaga atacgtaatg
    20401 aatattttcc cattgcgagt aaggactgct ggaccttcat ttactgaaaa accccgtttt
    20461 tcccattcat actcaggtat agaaaggagg atctgcttcc cctttaacgt ccaaggatta
    20521 tccatttcgg atagataaag attagaattc ccagtaattt caggatcttg ttgggcccac
    20581 acataatata gctttccttg agaagagaaa gttgttccgt ctaaactgaa agtttccctt
    20641 ttcgttttta tttgtccctt ttctatccaa tctgtattca ttggatctat atgctgattt
    20701 tcaagtacga acatgcggtg atgatgagat tcatcacgta tagttttatg attgcttgca
    20761 gcaaagtaaa tataccattt tccattgata taatggactt caggtgccca gatcaattca
    20821 ctcatcggtc cttcttcatg agcctgccat acaatcaggc ttgcagcatg ttctaattca
    20881 ttcagtgact ttgcccttct taattcaatc acttggtagc ctggaaccga tcctgtaaaa
    20941 taataataac catctgtatg tttatatacc caaggatctg cccgttgccg aacgatagga
    21001 ttattataaa gtgtcttcat cataacaaat ttcctcacat tttctttatt ctaattacat
    21061 tccaagagta tttttctatc cctacattta attcgtcatg tttttctatt gaaaaatagt
    21121 ttatttcagg ttttattttt tctggttctt cacgtgtatt tttgtccaac agatcgctac
    21181 tggttaatgt gtagtgctct ccttgatcta gcaaattgaa atcatgttca atcgttatct
    21241 tttgttgttc atttgtgcga ttgatcaaga aaatagtcag ctgattgccg ttatctacga
    21301 tcacactgtc cacataagga acatttttta tcttttcaca atcataaaca gggcaaatga
    21361 tttgttgttt caatacttga cctttagcat atttcgataa gtgttgcatc acataataag
    21421 ttggattcct ccatgcttgt cctttagcat ctgtcagtat taaaggtatt gtgtttacta
    21481 acaatgattg acaactaatc tttacgcgat ctgcgtgtcg taaaatagcc agccctaatg
    21541 aaccaaataa taatgtatct tccatagtaa agtaacacca atcgatggga ctaccgactt
    21601 caaaatccct atgtcttttt tccggttggg catgtacatt ccattcatca aacgctagat
    21661 acattgtttt ctcacttctc aaatatcctt ttacataatc acaagtagca acgatttctt
    21721 caatttgacg atcgaatcga taagcttttg caaggtatgt cgctgtatca tcacgctcat
    21781 ttttaggagg tttagtcaag tcttcttcat catacttatc aatatagtta tgcaaagcta
    21841 aataatctac attctcataa gcttcttcta atgcgatacg atcccattca gggtacgatg
    21901 gtaaacgtgg cgtagagctt ccaactaaga ttgtttcaat tgaatcatcc actaacttca
    21961 tgacttttga ggcttcattt gctaacctac catattcata agcactctta gcccccactt
    22021 gccatggacc gtctagctca ttccctaaac accataattt aacgccaaaa ggcttttttt
    22081 gcccattttt tattcttaaa tcactccaga aggttccttt tggaaaatta caatattcta
    22141 ctaaattttt tgcttcatca attccttttg ttcctaaatt caccgtgaat atcggtttcg
    22201 cttgtacatg actgatccat ttaaaaaatt cgtggagtcc aaaagaattt ggttctatac
    22261 ctcgccatgc taaatcaatt ttttttggtc gttgttcttt cctacctaca gtatccaccc
    22321 aatcataacc agatgtaaag tttcctccag gatagcgaat gacacctaat tctaattcct
    22381 ttacataaga gaggacatct tgtctaaacc cgtcaccatc agctgtttca tggcccggtt
    22441 cataaatccc tgtatagacg acacttccca tatgctcaac aaatgagcca aacagccgag
    22501 aactgatctc tcctaccgta aaatcctctc tgatttttac tttcacttct tcgtccaaca
    22561 gctttcccta cctttcattt cctatagtca agataataga tacaagtttc catacgccac
    22621 cagcaaagat aaaaataaaa ggagggaaaa gaaatatgag cagtacaaat cctaataaca
    22681 atacgcaaca aagaatattt ctcaaaaaat caaaaaatac tttccatgca ctttgttcaa
    22741 aaatttgtaa aaatgtgata tctttgtttt cagaaatcac atatatccag gataaaagaa
    22801 acatggaata aataagtaaa actacatatc cactaattga aagtacgatt gtctgttctt
    22861 ctaataattg attaacaaga aaataaataa tgatcgttaa cagactaagg aacaagttct
    22921 taccaaaatg ttgctttatt ccttgaaaga aaaaataaat gatatttcct gatccctcac
    22981 ctttccattg cgccattaca taaaaaacag cacatgtagc aggcacgata gtaataacag
    23041 gcaaacacac gattacccag agaataccta ataagatgta atcagaaacc acaatcaact
    23101 gtccataaag tttagtctca actattttca tcttcctcac tctttcattc cagacatagc
    23161 tgttccttga acaaaatatt tctgtgcaaa aatataaacc actagtaatg gcagcattga
    23221 aataaccgtt ccagccatta caggtccata aaaagaaatg ttctgccctg taaataaagc
    23281 taaccctgct ggtaaagttc tcatttttga actacttgtc aatatcaatg gataaagcaa
    23341 gtcattccaa ctattcatca gcgtgaaaat ccctaatgat aaaagagtag gtttgctcaa
    23401 tggcaacatg attttcaaaa aaatattaaa ctcacttaat ccatcaatgc gtgctgcctc
    23461 ttccaaagaa tctggtaaag taataaaaaa ggaacgcatc atgaagatac caaatgcagt
    23521 tgtcatacga ggaatgatca aacctgcata agtatcaagc atgccaagca aattgacttc
    23581 aataaataga ggaatcatga agacttggaa tgggatcatc attgtcagca aaacgaaata
    23641 gaacaaaact gattttcctt tgaaacgcat acgagcaaat gcataccccg caagagaatc
    23701 aaagaataca gaagtgatca ctgtccctcc agcaaaaatc actgtatttt tgatgtaatc
    23761 taataaaggg atcgttgtcc aaaccttaat atagttatct agggtatagc tatctgcaaa
    23821 tattctaggc ggaaatgaga taatgtcttt ttcgtgttta aaagaagaaa taatcagcca
    23881 caaaaaagga aacgcaataa ccaacaatat aactagcata ctaatcgttc gaatgatttt
    23941 tttaccacta actgcttttc gactattcac agtaactgta atcgagcttt ctggtgccat
    24001 acaggtttcc ctcctatttc atttgttttt ctttccgtga catgtacaca ttagtggaga
    24061 tagctatagt tgcaacaatg ataaacaaaa ccgaagacag tgctgatgca tagcccaatt
    24121 cataaggtgc tgtaaatcct cgactgtaaa tatattgaac agctgtttct gtcttaaatt
    24181 gtgggccacc agcggttgca acatatactt ggtcaaacac ttgcatcgaa ccaatcagat
    24241 tcgttaaaat acaaaaacct aaggagggaa cgatctgagg tagcgtaatg ttaaaaaact
    24301 gctgtcgatt tgttgcacca tccatttgag cagattcata caaagaggga gagattccct
    24361 gaatagcagt caagagaatc gtcatagtaa caccaaaatt cttccaaaca gtcatgatgg
    24421 ctaccgtcgg catagcaagg gttggatcgc gaaaaaattg agggatcggc aagccgactt
    24481 gtgaaaacaa gtaaggaatc gcccctacat tcggatcaag caacatagaa aaaataatcg
    24541 caattgacgt caatgaacaa ataactggaa tgtaaaatgt cgaacgacaa aaacgattga
    24601 atctggtatt tttagataat gctactgctg ctaataaacc aacgataatt tgtgttggcg
    24661 tttcgagtac ggtgaaatat aaagaatgaa tcaacgaatt aactgctcgt tcatcactaa
    24721 atatacgtgt gaaattttca aaacttacaa aatttcttga tgtaaagaaa atattcaaat
    24781 ctgtaaaact aatagcaaat gttcctaaaa gcggtatgac agtaaataat aataaaacta
    24841 aaaaagatgg agttaaaaac aaatacgcag cacgatcagg acgattccaa tatttggtga
    24901 atccgttcat atagtccacc tcatctttca ttttgataaa atatacaatc ttggtttaaa
    24961 cgaatctgaa taagatatcc gaacaatcgc tgtgattttt gcaataacac ctatctgcaa
    25021 atactaggtc gatcattcag atatctgtat cttgccaaca gtctatcctg tataaagttc
    25081 tgtactattt ttgcagtaat tgattgatgg catcatctgc atcgttcatg agtgtctcag
    25141 gatcttctcc tgctaataat ttttcaatca gtggattgat gatatcgtta ttgatcgcag
    25201 agatttgggt aaaacctggt aaatatggct ctgcatagtc catttgtttc gataattcag
    25261 atacaatagg atcatttttt acttcttcat cttttgctac tgaatccaag taggcaggaa
    25321 aaccattttc gatactccat tttttaccaa tttcagttgt attccaatat ttcaaaaact
    25381 catagacagc ttctttttta ttattatctg tacttgccgg aatggcatat cctacgccat
    25441 ctaaaatagc tgtttttttg tctgaatcac tcccttgtgg taaggtagtc acattataat
    25501 caatctcgtt tgctttcaat ccgttattta accatggccc attgatttca atggctagtt
    25561 gaccagcatt cattaaattg tccatttcgg cgccagaaat cgattctgga ccagtcttgt
    25621 ctacatggat cattttttgg atcgctttta acgttttaag gttctcagga gacgcaaatt
    25681 cagacttagt ataatcatcg ttcactaatt taccgccatt tgctaacatc cagttatata
    25741 acggtgcagt tccatcctta ttgaagacaa aacctgatac atttttacta ctatcagtta
    25801 attttggtgc catttctgtc aattcttccc aagtagtagg tggttgttca gtatctaatc
    25861 cagcttgttt aaacaaatca ttattccaat acatatacat gccttgtacc tgcatgggga
    25921 caaaatactg tttaccttct acttcaccta attcaatagc cgtttcttta aaatcagatt
    25981 tatctactcc atcatattcc cagaaatcgt ctaatggttg taccgctccg ttttctacat
    26041 actgtgcaaa atctggataa tttaatgcaa taaagtcagg tgctttttta gaagaaatcg
    26101 ctgtaggaag tttttcattc aaattggccc aagtcatgat atccatttca attttgatgt
    26161 ttttatcatt tgttttatta aattcattaa cgatttcttg caatatttca ccatcagatg
    26221 cagtaaaccc attccaaaaa gtcaaagtca cttctttatc agaatcagat tctgctgaac
    26281 ttccgccacc gcaagctcct aaaatacctg ttgcagctac caacaaagag ccaattaacc
    26341 attttttacc tttcattttt tgttcctcct tcatctgata aacgacatct gaccacattc
    26401 caagatagcg gtttcaataa acaattgatt ttcccatctt cagcttttac atttgttgaa
    26461 tgacttggtt ttacttgttc acttttagga agattagttg cttttgtatc aaatccactc
    26521 atttcagaaa aatcaatcac tccctctaat gataattcag acaagtgaac agttagattc
    26581 atttcttctt ctccacggtt aactgcaaat atgacaagtt ctttgtcatt tattacggct
    26641 atactttcta aatacggcac ttcttcaaaa tcttccgtcg catagtttgc tgaacgaatt
    26701 tgtgggctta acacctctcc ttgtccatac acagcagctt gcataaatgg ataatagatc
    26761 gtctgtctcc aagcttctcc gtttgtttct gtcctgattg gtgcaataac atttaccaat
    26821 tgagcaagac aagcaatctt tactctatct gcatgtttca ataaagtgat taatagacag
    26881 cctaatagta atgcatcttc aaaattatag ttatcttcta gtaaaggagg agcaatctgc
    26941 caaggcgctg cttgctcatc ttgttccgct gaatgatacc aaatattcca ttcatcaaag
    27001 gacaggttga tctgcttctt actatgtttc ttcgccttga ctgtatcgca catcgctgtc
    27061 acttcagaaa tgaaccgatc catgtctaag gaacgtgcaa gataatgaga aagatcattt
    27121 ttttggttgc cataatattg atgcagagac aaataatcta tttgatcgta cgcttgatca
    27181 agaactgtta actcccaatc accaaatgta ggcatttgcc gattcgaact tccgcataaa
    27241 accacctcaa ttgaatcatc tacgagtttc atcgccttag ccgtttcctc tgctaaccgt
    27301 ccatattctt ccgctgtttt atgtccaatc tgccaaggac cgtccatttc attccctaaa
    27361 caccatgtct taattccaaa agggctctct tgaccattcg aacgccttaa atcactccaa
    27421 taagttcctt taggaaaatt acaatattcg actaaattac gagctgcatc tactccacga
    27481 gtacccagat tgacagccat attgatttcg gcccccacat gctgacacca gttataaaat
    27541 tcatgaatcc caacttcatt cgtttcaatt gtccgccaag ctaaatccaa ccttctaggt
    27601 ctatttgcta tgggcccaat actgtcttcc caacgaaatc ctgacacaaa atttccacca
    27661 ggatatctaa tcatgggaat gttcaattct tttaccaagt gaatgacgtc ttggcgaaat
    27721 ccacttttat tagctgatgg atgatctggc tgataaatcc cttcatatac tgctcgtccc
    27781 atatgttcaa taaatgaact gtacaaccgc ggatcaactt tgcctacacg cttgacacta
    27841 ctaatcaaca ttgtggcttc cataactgac ctcctttaat ttatacatga ataatacacc
    27901 tcgttgaaag cgttgtcaat aatataaatg ttaaacttac ttatttttta tcgattttaa
    27961 ttaaccaaaa tattaataat gattaaaaca aataatttaa tataactttt attaacaaat
    28021 atgttaatat ataagaaaaa ccttttaaga ggtgatatat atgcaactac aaataaacga
    28081 agaatcctta ccggtatatg aagcattagc tagtaaaaca aggatcaaga tcatccagct
    28141 actttccaaa aaaaagatga atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc
    28201 tatcacgacg atgcacgtga aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt
    28261 aggacaacaa aaaatttcga gccttcgtgt cgataaaatc gatatcagtt ttccagaaaa
    28321 aatattcaat gcttttgata cgaaagaaac atcgatacct attggtcact atacaaatta
    28381 tgcaattgaa ccaacatgcg gtttagctac gatccatgat tttatcggga aagtggatga
    28441 accacgctat tttatggatc ctcgaaggat ggatgcccgc attctctggt ttaccagcgg
    28501 atttgtcgag tatcaggcac ctaatttttt aaatacagag gatacattgg agatgttgga
    28561 agtttctgtg gaaatttctt ctgaatttcc tttttcaaat gacaattggc cttcagatat
    28621 cactttcagc ctaaatggtg tggagctagg aacatggacg agccctggag attttgctga
    28681 tatccgcggt aaatatacgc ctgattggta tccagataat cttaatcaat acggcttgct
    28741 gaagacgatc cgaattacca aacacttgac gaatatgaat ggagaaccct tgtcaaacat
    28801 cacgatcaac gacatcccta aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga
    28861 cgatgccaaa catgtggggg gctgtacttt atttggtaaa ggattcggta attacgatca
    28921 ggatatcaaa ttaaaagttt actacagctg atatttttct tttctatttt ataaaacaaa
    28981 aacagccaat ccctatgaag ggacggctgt ttttgcttta ttgttatttc cagaaatcat
    29041 ccaaaacgga aattggcaaa tgtcgtttat gttgtgtctt gttccaccat gcttcgattt
    29101 tttcttgtgc ttgagcggga acttcttttc cttctagata atcatcaatt tcattatatg
    29161 tgacacctag tgcgacttca tctgcaatca ttggtttccc atcttcaaga tctgcagtcg
    29221 gaatttttgt gtataatttt tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc
    29281 gtttgtccaa acggaataat ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga
    29341 atccagtaat gttttctgca gcatgatctg tccctaatac ggctcctgct ctttctccag
    29401 ctacggcata ttgcgtaatc atacgttgac gtgctttgat atttcctttg ttaaaatcgc
    29461 tgattcccac acctgcttcg cttaatactt gtacttgtgc atcaacagct ggtttgatat
    29521 ttactcgcag actaacatcg ggctggataa agtcaagagc agcttttgca tcttcttcat
    29581 ctgcttgttc accataagga agacgaacag cgataaattg atagttttca tcttttgttt
    29641 cttcacgcat ttcttccatc gttagttgcg ccaaacggcc tgctaaagta gaatcttggc
    29701 cgccgctgat acctagtaca aaagttttaa gaaacggata ttttttcaaa tatgctttca
    29761 taaagtcaat acttttacgt atttcttctt ttggatcaat tgttggtttg acacctaatt
    29821 cattgataat ttctgtctgc aggctcattt attctcctcc tatgcttctg tatgaagttc
    29881 tgctacgctt tttttcattc gttccatgat agccatttta tggttccagc attcagtaga
    29941 cagatcgact gggtattttt gcggattcaa gtctcgttta tactcttccc atagtgaatc
    30001 tagattttct tttgtatatt ctttgatttc gtttagtgca ggtaattcgt aaactctttt
    30061 tccttcgata aagatatctt gtaaaactgg gcgagctgta aagtctcgaa cagttttatt
    30121 gataaacgta tgcactggat ggaacatgaa gatagcttct tcttctcgag ggtcttcatc
    30181 ccataatgtc acgtagtctc cttcagattt cccatcaaaa tttcgagtga tgcgccacac
    30241 ttgttttttc cctggagtag ttactttttc tgcattgctt gataacttga tcgtatccat
    30301 catttttcct tcatcatctt cgatcgatac tagcttgaat actgcaccaa gggctggctg
    30361 atcataggct gtgatcaatt ttgtcccaac accccataca tcaattttag ctttttgcat
    30421 ctttaaattc aaaatcgttg cttcatccaa gtcattcgaa gcatagattt ttgcttccgt
    30481 aaatccggct gcatctaatt gttcccgaac acgcttagat atataggcca tatctccact
    30541 gtcgatgcgt acgccttgga agttgatttt gtcacctaat tctttcgcca cgcgaatcgc
    30601 acttggcaca cctgatttca acgtatcata agtgtcgact aagaacacgc agtctttatg
    30661 ggttttagcg taggccataa atgcttcata atcatttcca taagattgga ctaacgaatg
    30721 agcatgggtc ccgctagctg gtatcccaaa tatcttccca gcacgtacat tgcttgttgc
    30781 atctgctccg ccgatatatg ccgctctagt tccccagact gctgcatcca gttcttgtgc
    30841 ccgtctagtt ccaaattcca atagtggatc ttctccgatg acagatttga tacgagctgc
    30901 ttttgtcgcg atcaacgttt ggaaattgac catattcaac agtgctgttt ctactaattg
    30961 gcagtgcgct aaaggccctt ccacttggat taatggttca tttgcaaaaa ctagttctcc
    31021 ttcacgagca gaacgaactg ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc
    31081 ttcaggatag acttctaatc cacgcaagta agcaatatcg gaatcattga acgtcagatt
    31141 ctctaaataa ttgactaacc gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg
    31201 catttctcga aagtaacatt caaaaacagc atgtaaatca gctcgaccta attcccagta
    31261 tgtctgcatc atattgattt gatatagatc cgtatgcagc gtcaaactgt cgtctgggta
    31321 agtattattc atcttgtatt actcctcttg gcaagttttc atttcctacc cattataaca
    31381 aaaaaaatta ataggaaaag aaatcttcca ttttttctga aaaagcacat gttttacgga
    31441 tatttcttgc taaaaatgaa ctccatctgc taaaactatc ctttcttatt ggtatttaac
    31501 ctaaacgaga aagaatactt attttattca atgtatccga gacacgaaga aattagcgtt
    31561 ttctattttt cttacttgat aaaataagta gattttccta ctacaatcgt agccgttaag
    31621 ataccgatga ttgaacaaat ataaccaaat gtctctacaa cataatcaat ggcaaaagtt
    31681 tttgaagcaa taatacgtct ttcctttttt gcttcttatc cccaaagaaa cggatataac
    31741 ttattatgta ttgaattttt ttggaaaggc gaattatgac aagatatacc ttgcgttgaa
    31801 aaaactcgcg gaaaaaatat cacacgtttt taaaaagata caaagtaaga aagcagctgt
    31861 caatagaatg gaaacaaaaa tggcgagaca tcaatcagtc tcgccttgtt ttttattgta
    31921 tacattagat atggagcagg ttaccataaa gatgattatt agtcgttatt aaatagaatc
    31981 cttcgtattt ttttgttcta tcactacaat atctttattt ggacgtttga attgtattcc
    32041 ggcacgttct agctcctgtt tcactcctac atggactttg acatcttctc gtgtcatttt
    32101 taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg atttttttct tcgtattgat
    32161 catcacgact tgctttttat catatgtgtc taccaatagc agttgtgttc gatctaacga
    32221 aaataatttg taataattaa tagccgtatt cccaaaaaag aattccacta ctttaggata
    32281 aaatgtctta atatttaaat tactattcac aggtgtttcg attagcttca tctcattcct
    32341 cttttctata tagtcgattt agatccaatc ttaagttatg ttaccatctt tcctagcatt
    32401 aaggaatcgg ttacaattga acctttaaag acaaaaggat ttacctttta tcacttaata
    32461 gtataacata atttcgtgag aaaaaaatac aattttttct tgaccatctc gttacgtaaa
    32521 ggattatgct tacatttgtc aggaggaaaa ataatggagt acacaataaa gaaaatgtca
    32581 gagatatcag gagtcagtcc gagaaccttg cgcttttatg atgagattgg actgctaaaa
    32641 ccagctagga tcaattcttc aggttatcgg atttatggca aaaaagaagt cgatcgtctg
    32701 caacacatct tattttatcg aacaatggca ttcaagttgg atgatatcca agaggtctta
    32761 gacaatccat cttttgacca ccagaaagct ttaatcaagc atcagcaaat gttgctggaa
    32821 aaacgggcac aaatcgatac cctcttgaca actgtccaac agacacttga tatgtacgaa
    32881 ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca aaagttgaaa
    32941 gaaaatgaag aatcctatgg acaagagatt cgacaaaaat acggaaaaaa aaaggttaca
    33001 gcatccaatg aaaaatggat gaatatggat aaagaaacgt atgaagaaat gcaaactgtt
    33061 gaaaagcaga tgttatttga tctcactctt tacttaaaag aaccagaaga tgaaagctta
    33121 gcagaccgta tcttccaagc tcataagaag tggttctctt acagctggcc tgaatatcag
    33181 ccaaaagcgc ataaaggact aggaatgatg tacgaagccg atgaacggtt cactgcatat
    33241 tatgacgagc gaagtggtca tggtgcagca aaagctttaa atgaaattat ccaaaaaaag
    33301 gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa taacaaaaca
    33361 gcagacaccc aagttagatt tttatgctaa cttttggtgt ctgctccatt ctttcacaag
    33421 ttttttcatc ctactttaaa tatattcatc gttgaataaa cgtataaggt attttcactt
    33481 tatccttttt ttctgtaaga aacaattgaa atgccatttc tccgcaagct tccagatggt
    33541 ggtcgatggt tgggaaacca agaagctcgc tgatcagcaa attatcgcgt ccaatcacca
    33601 ctggtgtctt atcttgcaga tagctttgca agatgcctgc tgcaaccata tccgaatttg
    33661 taaagacacc atcaactcca agctctttaa aaaagaagcc tgcttgtttt cctttctctg
    33721 catctataca atcccaaaaa acaagtgatt catcaaaaga tggaaaaagt tgatgacata
    33781 gctgcaaagt gatttttgag ttataactta gcctgccact tcgccccaac gtgatgccca
    33841 attgctttat accttgctgc ttcaaataag taagaccttc tgtaattgat tgttctcgat
    33901 caataaagac acagcttgcc tgttttccct tgatttcttc acaaaaaact agaggtccat
    33961 atttttgata tgccaataaa tcatctagtg tattggcacg agacgtaatg atcaatccat
    34021 cgaaagcttt agctgcaaat tcttcaagat accttcgctc tttttcttta tgatagcccg
    34081 tcggtaaaag tgtcactttg taatcataat caaacgctgc ttcaatgatc ccactgatca
    34141 actgatcaaa atacggatga ttggtataag gcagaatcac tccaatgctt ttcgtttttc
    34201 cataacttaa ctgtctggca tttgcattag ggatataatc gacttctttc atgaccgcaa
    34261 gtatttcttt ccttttttcc tcatctacat atggatgtcc attgattaca cgtgaaatag
    34321 tagaaactga ataacctgtg agctttgcga tatctcgaat gtttgccact gctgcttcgc
    34381 cacctataca aaaagatttt cttgtaaata gtctaccaca gctggaccgc attcaaaaaa
    34441 ctgatacatt tttccttcat gcgaaaaact gatccgctgc ccgccccaat atatatcttt
    34501 tgtcaaattt tcgatttcat tcattggtat ttcttttact atattcctat tcaacccttg
    34561 aaaactgtac aacgttaata cggatccctt gaactcgaca aacgcataaa aataattgtt
    34621 cgttgtgcca tctgttatct ctacataagt attcattgca agccctcctt ttgatacttt
    34681 gagtatagtg tatgaaacgc gtctcatagc aaggagtttg ttttgccaaa gaaaagtttt
    34741 attcctcttt cattattacc aaagtctctt attttaaatt gaatacatta taatgatact
    34801 gtaaatatat ctttattatt agtgaatgga gtcaagtcca caatcttgtg taaaagttca
    34861 acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct ttctttctga
    34921 ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag aaagaagaga
    34981 ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat tttcgtggag
    35041 aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga gtgatggaat
    35101 tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg ttcaaccgtt
    35161 ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa
    35221 tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac
    35281 catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa
    35341 agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa
    35401 tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa
    35461 cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc
    35521 gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg tcttgaacat
    35581 caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata tatacacctt
    35641 ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac atgaaaaatg
    35701 gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga tccaactgtt
    35761 tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc agttcaactg
    35821 ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg gcagcgatca
    35881 aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta ccgtctctgg
    35941 ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga tcgtagtaac
    36001 catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga atatattgat
    36061 ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt gcggtatcat
    36121 tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta aaatgtactt
    36181 gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg tactcctaac
    36241 tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg taatcgtgaa
    36301 tggatattta cttttaatag ataaaatatt ataactatta aaaataacta ttttcattat
    36361 aattacacac aaaaaagagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    36421 acacgtataa acagcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    36481 atttgaaaag caatttccgt gaatttcttc ttatttcttg gagttaaacg cttctgttcc
    36541 agcctctttt tatctgtctt tcactaatga tcagttgtag ataagcaagt tgctttttct
    36601 tacatcatac cgcccatcat tgatggatcc attgcaggag ctgctggcgc tgctggttct
    36661 ggtttatctg caacgactgc ttctgtcgtt aataacaagg cagaaacgga agcggcattt
    36721 tgcaaggcag aacgtgttac tttagttggg tctacgatac ctgcttcaac catgtttacc
    36781 cattctccag tagcagcatt gaaaccagtg cctagttcca cgttcttcaa tttatcaaca
    36841 atcactgatc cttcataacc agcgttttca gcgatttgac gaattggttc ttctaaagca
    36901 cgaacaacga tcttgatacc tgttgccacg tctccttctg cttctacagc agaaactttg
    36961 ctgataacgt tcactagagc tgtaccacca cctgagacca ttccttcttc tactgcggca
    37021 cgtgtagcat tcaatgcatc ttcaatgcgt aattttaatt ctttcaattc tgtttcagta
    37081 gctgcaccaa ctttaacaac agctacacca cctgctaatt tagctaagcg ttcttgtaat
    37141 ttttcgcggt caaaatcaga agttgtttca gcaatttggt ttttgatcaa ttgcacacga
    37201 gcttcgattg cttctttttc tccagaacct tctacgatcg ttgtattgtc tttatctaca
    37261 acaactttag aagcatttcc taagttttcg attgttacat ctttcaattc taaacctaga
    37321 tcgtcagtaa ttactgtacc acctgtcaaa atagcgatat cttcaagcat tgctttacga
    37381 cgatcgccga aaccaggagc ttttacagct actacgttga atgttccacg gattttgttc
    37441 aatacaagag ttggcaatgc ttcaccatct acatcgtcag caataatcaa caatggacgt
    37501 gattgttgca agatttgttc taacaatggc agaatatctt ggatgttaga gatttttttg
    37561 tctgtgatca agatatatgg gttttctaga acagcttcca ttttgtcgtt atctgttacc
    37621 atatattgtg ataagtaacc acgatcaaac tgcatacctt ctacaacatc tagttctgtt
    37681 tcgatccctt ttgattcttc gattgtgatg acaccgtcgt ttccaacttt ttccattgca
    37741 tcagcaatca aatgtccaac tttatcagaa cccgaagaaa cagctgctac ttgtgcaatg
    37801 gcttcttttg aatcaacaac agttgagata ttatgcaatt cttcgactgc tgcttttgtt
    37861 gccaattcga tcccgcgacg aatgcctaga ggattagcac ctgcagttac gttttttaaa
    37921 ccttcgcgaa cgattgcttg tgtcagaaca gttgctgtcg ttgtaccgtc tcctgcaata
    37981 tcatttgttt ttgatgctac ttctgataca agttttgcac ccatgttttc aaaatgatct
    38041 tctaattcga tttcttttgc gatcgtcaca ccatcatttg tgatcaatgg agagccgtaa
    38101 gatttttcta gtacgacatt tcggccttta ggtcctaatg ttactttcac tgtatctgct
    38161 aatttatcta ccccgcgtaa catagctgcg cgtgcgtctt ctgcaaattt caattctttt
    38221 gccatgattg attcacctca ttgattattt ttgattattt ttttatcttc caaatttact
    38281 caacaatcgc catgatatct tttcctgcga cgatcagata ttcttttcct tcgtatttta
    38341 cttctgtacc ggcgtatttt tcgaacataa cttggtcgcc tgcttttaca gcggcaggaa
    38401 ctttttcacc gttttctaac aaacggcctt caccgactgc aacaactgtc ccagtttggg
    38461 gtttctcttt agcagcagaa gctaaaacga ttcctccgac agttttttct tcttcttttg
    38521 cgacttcgat aatgacgcga tcacctaatg gttttaacac gataaatccc tccgataact
    38581 ctttttttaa tctgttagca ctcttaacct tcgagtgcta acctacattt ttaataatac
    38641 tcattctccc tcttttttgc aagcttttaa atcaaagaaa agaaatttta ttctgacagc
    38701 tgcttattga aattatgata taattgaaat caatcgatat aagcttgcct tgcatgcttg
    38761 ttatccctta aaaggaggaa ttacatgtct ttaaaaaaat atagcttcct cagcattttt
    38821 tgctatggtc taatttttat ctcacccttg atcctagctt cgataggact tgtaaaatct
    38881 acgtctgaat tgatcacagc aactgcagtc atgtatatac taggcgccgt tgtcctagct
    38941 attttttatt ttaagcaacg tgaaccctta gcgatcgaat cagcggtaaa aaaagcttct
    39001 gctccaaaga ttgttattta tggattggtt ggaatctttg tcgcattgat tctgcaaagc
    39061 atcgctgtca tgttagaatc tgtattgttt ggcaaagcaa ccccttccga gaatacacaa
    39121 aatatcatcc agatgatcat ggaagcaccc gcttttattt tggcgacaac tgttgctgga
    39181 cccatcatgg aagagttcgt attccgccgc agtatactgg ggatcatcag tcgatattca
    39241 aatttttggg tcggagcagt gatcagctct ttgctgtttg cgtttgctca taatgatggt
    39301 catctgctaa tctacttttt cctaggtttc ttcttcagcc tagagtacaa agcaactggt
    39361 cggatctgga catcgatgat cacacacgta ggaatgaaca cattagttgt tcttgttcaa
    39421 ttagctgtcc aaaaagggct agtataaaca tgactataaa tactcctgtc atcctagatt
    39481 ggtgattttc ccaatctaaa gcgacaggag tatttttgtt ttttagtcgt tcatcttttc
    39541 tgctgctcca tcattttttg tctgtctttc ctttttgcag tttgcagaat ctattttttt
    39601 caaaaatggg aaataaatga aaaaactaat tgacaacacg ataccttgta aaatcgctgc
    39661 aggcactccg cctactaaga aacccgagag gatcggcggt gttgtccaag ggacttggac
    39721 tgctgtaaat aaaggaacga atccaaaata taaagcggaa taggtaatca atcctgaaac
    39781 gaccggtgcg aaaataaaag gaacagccat catcgggttc atcacgatcg gtgtggaaaa
    39841 aataattgat tcattgatgt tgaaaaaggc tggtaaaaga gcaagatttc ctaattctct
    39901 gtatttcttc gatttcgcaa aaaccgtcat aaaaaataca gcccctaacg tcaatccagc
    39961 acccgtaacc gtcatgaact gatcgagaaa ttgctgagta acgatatgtc cgccatttgc
    40021 caccgtcaac tcttgtcctg aggctaagat ttctgcattc gctaatgaat tcgcttgcaa
    40081 gacgggacta accaacccat tgaccatcgc agatccatga agaccaaata accataaaaa
    40141 cggaacaaaa aaacctaata aaagcacacc aaacaacgtg tctatgaacg attgaatggg
    40201 aagctggaca tatgtccata ctccttgaac aacagtcatt ctgaacaact gttgaaaaac
    40261 cgctgaaccg ataagcgtaa aagtgactat aacaatgatt ggaatcaatg aagtaaaaga
    40321 ctgccaaaca ttttcaggta tcttctctct ttgcttccac cctttcgcaa gtcttactaa
    40381 acgtgcatag ccccatccga tgatgagccc agttgtaact gctcctagta tcccttcagc
    40441 acctgtccag acagtatcta tctgagtatt tagtggctga ataagcagta gataagcaga
    40501 caaggctgtt atgccgactg gtaagcgatc gatgtcttct tcttttgcat aagcgtatgc
    40561 aatggcaaaa acgatccaaa aggctatcac atcaaatgta gcctggtata cttgtgtaaa
    40621 atacatagaa aatcctgttt tgtctatcca ctgagcgatg taatccacag gaaaatgagc
    40681 aaataagaaa aacagcgagc caacaataat tattgggatc gtgtaagtca ttccattacg
    40741 caaagcagtt aagggtctaa gattcacaac agatgttatc attggtaaaa aggcacgatc
    40801 taaccactct ttcatttctt atctccttca acttctattt ttatcaatca gttttcttaa
    40861 tttaactata cccaatgatc acgaaccatg aaaggaatgc gcactgagct tttcaaaaat
    40921 cggattcttt gacgaatatt cagcaactgc ttacaatgga caggaataaa aaaggaggtt
    40981 ttttatgaaa attgcactca ttggagctac tggccatgca ggttcattta ttttagacga
    41041 agcattgtcc agagatttag acgtgacggc aatcgtgcgt catccagaaa aattgccatc
    41101 agatgttccg tttatacaaa aggatttatt tgagttgaca actactgatc ttgcatcttt
    41161 tgatgttgtc gttgatgctt tcaacgctcc caaaggaaaa gaagaaatgc atcaaacttc
    41221 tttgtcccat ttgattgaga tgcttgaagg aaccgataca cgcttaattg tcgttggggg
    41281 tgcctcttcg ttattcttag atacagaaaa gacgactcgt atgattgacc aagtacctaa
    41341 aaatgcaccc ttctatccaa cagctttcaa tatgtacgaa ggattgttaa aattgaaaca
    41401 atctaaaaat ctacattgga cttatatcag tccagcttct ttatttgatc cacatggcaa
    41461 acgtacaggt acttatttgt taagtgatga ttatttacaa agaaacgctg ctggcgacag
    41521 tacgatcagc atggcagatt acgcgattgc attagtagat gagatactgg atgagaaaca
    41581 tgaaaagcag cacatcagtg tagtcagtcg ataatacggt tattttttta atatgctaaa
    41641 aaaataaaaa tagaagataa aaccaaccct gattgatttt atcttctgct ttttatattt
    41701 ttattgattg aactgtttaa atcgaaaata gctcagttgc ttcatctgga tctgtatcat
    41761 agatttcaaa atcatgtcca acgccaaaat ctttttcttt caaaacattt tccattgtta
    41821 aatgagcaag ttctttcata ttgttttcct tgctaagatg gccaagatag attcgtttgg
    41881 tccgattacc gatcacatca gtcatgacaa gtgcaccgtc ttcgttggaa agatgtcctc
    41941 tatctcctaa aatacgctgt tttaaattcc acggataagg tcccatgcgt aacatctcaa
    42001 gatcatgatt actttccatc aaataaccat cggcattttc aatgacacct cgcatataat
    42061 cactgcaata tccggtgtcc gtcaacatga cgaatgaccg attttcttta tggaaacgat
    42121 aaaattgcgg agccgctgcg tcatgggaga cgccgaaact ctcaatatcg atatcgccga
    42181 atgttctgac tttccccatc tcaaatagat gtttttgttc cactggtaca tttccaatca
    42241 gtggttccat cgcttcccaa gtctgctgat tggcaaaaac atccaaatga tacttacgag
    42301 ccaagacacc cacgccatgt atatgatctc gatgttcatg cgtcacaagt attccatcta
    42361 attggtccgg tgtccgatca atttgagcca tcaaggaagt gattttttta ccgctaagtc
    42421 ctgcatcgac caaaagccgt tttttttcac tttcgatata aagtgaatta cccgaacttc
    42481 cacttgctag gatactgatt ttaaaagctg gtttttcttc agccatttga ctcctacttc
    42541 ccttcttact ttgtaaactt ttttcattat actcgctttt ttattgatct tccacttttg
    42601 gtacagtatt gtttgtcacg atcgtattgc tgaaagcgtt gacactctca atttggacca
    42661 ctttatcgct tgtttcgatc gctacatgcc aaacaggaac atacacattt ttttcccgga
    42721 ctttcagaat acaggaataa gctagctgac gccataaaat ttttgatcct cttggtattt
    42781 tgttattgat gtacaatgta ttgattgctt cctcttccgt atacagttcc gtcttttcgc
    42841 gcagctgctc gatatcagat aaatgcgtct gcatgtattt cgtgatgcta taatcattat
    42901 cgtttttttc taaagaaaga acgatttttg ctgtatcatc attgaatggg atgccttcat
    42961 actcttggct tgcttggatt tctagtggtt cttcttctaa atgagaaaag tttttcgtgt
    43021 acacataatc attaccgaat aaaatattgt ctttgttaga taaaaaatct tccaaggaac
    43081 tactaatttt atctggatcg atcacgttct tttcagcatc tgaactggat aaagatttcg
    43141 tcaaaacatc accgtctaaa aacacaccac ttgccagcaa gccgctttta ccagccttgc
    43201 gataattcgc taatgaagtg ctaagattgg tctgctccgc gctaagataa tagccttgct
    43261 tgttctcaga cgaaatcttc cccgtgtaag tgatatcgtc gctagccaga cgttgttgga
    43321 ttgattcagt ctgattagag cgaatgacat tgttttcttg gtataccgcg tcatgataac
    43381 tgctgaataa aaacacattc aaccctaaaa aagcgacgaa aaatatccat tcgatttttt
    43441 tgaaatccaa ttaattcgcc tccaatccag gtaatttttc cagaaggtcg ttagctgaat
    43501 accagttatc tttatatttc acataccatt caggtaagag atcaacgaca cgattcgttt
    43561 ctttgacatt ttgccatgta tagccaacga tcactgaacc aatcttgttg ctatctgctc
    43621 ccttagcttt taactgctgg atgatttcat tcgtacttgc aagttctact tcttcgtctg
    43681 aaggtatcgg cacttgaatc gtattcaaac tggtttggat attcacttgc acagaaccat
    43741 tcgtttcacc gccaacttcc acaccaatct gtcctctgct gtctgtccca aaaaccggga
    43801 agccttccac gaatatccga taatcgatcg tattgtcact gcggtcaaaa tatctgaggt
    43861 tccctaaaga actgcctaat ttgctaatat aggggaaact ttcagaaaat acactagcat
    43921 tttctttttt ctctggcagc tctccacgaa aatcaactac ttgcgtttcc gaatggatag
    43981 acattgtttc attcccaccg taaaaaatca attcattgct ttcatcatta tttttcacgc
    44041 tatctggttt ctggaaaaaa gcatttctaa aaagtgtata gggttgctgt gacaggatat
    44101 aactatactt tttcagtttg atcgaatgtt tcgtagcata ttgcgtgtcg atcgtcgatg
    44161 gttctgttgt catttctgtc cattttgtgt cttttgctgt taattcttta ttgatatctg
    44221 tccaattcaa cgaaatattt gcttcatata cgagatggtt cgaataattc aaaaatcgga
    44281 tttttttatt tccttgatcg aattgtattc tagtaaaata catattttcg ctttcttttg
    44341 ccaagttcga cacatctaaa tctaaatcaa atgcttcctt gtattctctt agcaaaaaag
    44401 gcgcattata ggccaactca aaaccagagt tcagattttt cacctgttca aactgtgatt
    44461 tgtctcctga aactacttca gaaagctttc caaatcttgc tttatcgatg atcaattgca
    44521 atcggtacac gagattttca ctgttggttt cttggatcat tcccgaatgg tgccatgtaa
    44581 catgaagcgg aagaaagact tcggaagttt ttcgattgct ttgagactcg atcatttgag
    44641 tgtttgtctc atctacgttg attgaacttt ttcctgctgg gctcagccag attgcataag
    44701 agaagtatat gcttaacgca accatcaaaa tgagtcctat acgaacgact ttttcagaga
    44761 ttctcattcc caccattcct cctcataagg ttcatatggc agcgagatat aaaaagtcga
    44821 cccttttcct tccttgctct ctgcccaaat agcgcctcga tgcgccttaa tcacttctct
    44881 agaaatagct aatccaagtc ctgtcccacc ttgtgctctt gcacgtgctt tatctactcg
    44941 atagaaccgc tcgaacactc ggttcagatc ttttttagga atccccaatc cttgatcggt
    45001 gatacttaaa accacgttat tatgcgtttc taataagcga caagtaatcg ttcccccatc
    45061 tggtgaatac ttgatcgcgt tattcatgat attatcgact acttggatga ttttatctgt
    45121 atctacttcg acccaaagat ctctttgcgt aaattcacga cggatcttat aatttttttc
    45181 ctgatttccc accatcatat caaaccggtc aagaacaaaa ttcactaact cgttgaaatt
    45241 gacatattct aattgaagct gggtattacc tgtatccatg cgagatagat ttaagaggtc
    45301 attgatcatt cgaatcattc ggtctgtttc atctagcgtt accttcaaaa aatttggtgc
    45361 tacttcctcg tctttccacg caccttcgct caatgcttcg atataactac gcatacttgt
    45421 caacggtgta cgaagttcat gggaaacatt agagacgaat tcacgacgtt cccgttctgt
    45481 tttttcttgt tctgtcacat catgcaagac agctactaat ccgctgataa aacctgattc
    45541 gcgacggatc atagaaaagt ctactcgcaa gatcatctga tcactttcca atgtactggt
    45601 tgaacggtca agtaccattt cgtttggatc ttctaataat ttccgcaacg tatattcttc
    45661 ttcgatatct aaaagtgtca aaatcgattg tccgattgct tcctcgtttt tgatatctag
    45721 caaggaaaca gccatatcat tgatcgtaat gactttaccc ctgcgatctg tcgcaatgac
    45781 gccgtctgtc atatgggata gcacgctatc caaacgattg cgttcagctt ccattgtatc
    45841 ttgagcttct tcaatacgtt cagctaactg attgaaggta tcagccagct gtccaagttc
    45901 atcttgtcca tgcactttga ctttccgact gtagtctcct ctagcgattc gaatggcttg
    45961 ttctctcatt tcaccgatcg gctgtgtaat cgaacgagca actaaaacag atacgatcat
    46021 tgaaataaat gccgcaatta gtgaggcagt aaagaaaatg actgctgtgt tattgatttc
    46081 cgaatatttt tgttcgatat tacttttgac gtaaagagcc ccgataacgg catcgccggt
    46141 cggggacagg atcggttgaa cattgatatt cacccgttta tcgtcgttat ctaaagcaac
    46201 ataacgttta gtcgtgaaat cattgatatc aacataatca tttttctttc ccacagcgct
    46261 ttgttcattg acatcggttg ttcctcggat gatccctttt tcgtcaacga cccgtatttc
    46321 gatgatatcg ctagtcgcac tgttttcaat aaatcttttg atattcgcat ttacctcttc
    46381 atcatctgca ttatcttttc gattcaattc cgggctgatt gttgttgcta atgattccac
    46441 cgtttgattc atatctttaa taaatgtatt gatcgtggaa cgttctaaac cacgtataaa
    46501 gtaggcacca ataatttcga tcgagatcaa taaaatcaga ataaaggtaa tagcgatctt
    46561 aaaattgact gatcgaaaaa agcggacttt tcttttcatc caattactcc tgttctgggt
    46621 tacgcaaata gtaaccgaca ccacgacgtg tcactaaata atttggatgg cttggattat
    46681 cttcgatctt ttcacgcaaa cgacgtacag ttacatccac tgttcgtaca tctccaaaat
    46741 aatcataacc ccaaaccgtt tgtaataaat gttctcttgt catcacttgt ccaaggtgct
    46801 tcgccaagta aaagagtagt tcgaattcac gatgtgttaa ctcgattgtt tctcctcgtt
    46861 tcgtcaccat gtaagcttct ggatggatcg tcaaatcacc aatcgtcaat tccgcaggtg
    46921 ctgcctcttc tggttccttc gcagccgtgg caccgcgacg aagatttgct ttgacacgtg
    46981 caactaattc tcggttggaa aatggcttgg tcacgtaatc atcggcgcct aactctaagc
    47041 ctaggacttt atcaatttca gaatctttgg ctgttaccat aatgatcggc atatcataag
    47101 ttttgcgtac ctcacgagca acttctaagc cgtccatttt aggtagcatc aaatcaagta
    47161 aaatcaaatc aggttctacc tcagcaactt tttccaaggc ttcttcccca tcatatgcag
    47221 tataaacatc atagccttct ttggctaaat tgaatttgac gatatccgaa atcggcttct
    47281 catcatcgac aactaaaact tttttcataa agagccacct cttatatatt attgtcatac
    47341 cttctcatta taccgtattt ctgcttttgt ttcatctttg cctataaaac aaaagagaaa
    47401 attaaaatga aagtgcaaaa tactttatta ttttttgttc taacttcaat aaaaaaggaa
    47461 aacactgatt ttcacctcag tgtttccttt ttttgattta tccttctaaa acttgtacac
    47521 cgacacccaa taatcccgga ccagtgtgga ctactaatgc cggagaaatc tggccaaagt
    47581 agatttggtt tgcttgtggg aactctcttt taagtctcgc ttccatttca attgcttctt
    47641 ctttcgcatc cccatgggca acagctaagt tgaagacctt tgcattaccg attttttctt
    47701 tgacgtaagc aaccgtttta tccaaacttt ttttacgtcc cctagctttt cctactgtgt
    47761 aataaatccc ttcttcatta catgagatga ttgggttaag ctttaaagcg gttcctacaa
    47821 tagaagcaac taaaccgatc cgtccacctt tttgaagata ttccaatgtc gcaacattaa
    47881 agaaaacttt tgttctttcg actaactcat tcagattact gatcagttga tcccattcta
    47941 ctccttcttc cagccatttt gctgcttgga tcgcagaaaa acctgcacca ataccaatat
    48001 ttttcgtatc tagtacatat gcttccaatt cttcttgctg ttgtgcaatt aaacgaacca
    48061 cattatatgt cccgctcaat ccactggaaa ttgttaccgc aagtaccttt tcataaccgt
    48121 cttttttgat ttgctcaaag atttctgtga tcgactcacc atccggcaaa gatgtaccag
    48181 ggatttcctc tggcaaacgt tgatagatct cttcagaggt aatgtcaatt ttatctgtat
    48241 atacctgatc tttataaata attttcaatg ggatcatata catctcgtat ttttttatca
    48301 attcttctgg cacatccgta cctgaatcta ctaaaattgc gattttattg tttttcattt
    48361 gttttctcct cttttaaata ttcggtttct agaacaatcg ttttttccgc aagaagtttc
    48421 attgcaaacg ataaagttgc cgctcttaca gcaatatatt ctacaggggt tgcctggtcg
    48481 aatttttgcg gatgagattt accttgtgct aattggctga ccatccacac agctgcttct
    48541 tgttcatcgc aaaatagatt ataggcttca cgaattccta ctgtttttcc ttggaacaat
    48601 atcccttctt tgatttcagg aatagtcaat acttgtttca acgtagtgat tgcaagtaaa
    48661 aatgctacgt gttctttgtt atatcttttt ttaactggtg ctggtatcaa acctagttta
    48721 acgtaattgt ttaccataga agaggtaagc aatggatgct tttctccttg tatcacaggt
    48781 gaaagataac gatcaactaa cgttctgact tggtccatat aaagttctaa gtcagggagt
    48841 tcttcccagt gaggcagacg gaatgtttct agttccttcc cccactccat taattgctga
    48901 cgcatttttt ccatcataac cgctcctttt ttcatttcat aaacatgata tcagacttat
    48961 gataaacatt caatctagtt gctttaacta ggtgatttga ttagcgggat tccatgaaaa
    49021 caaaaaacaa gaaggctaag ccttcttgtc ttttatcgtt aagaacagga gacccgccgg
    49081 ggagacggcg ggaaaggagt aaaaaatgaa aaaagtgtta gggtttgttc tcttgcaaga
    49141 actttgtttg ttggtatgct tatataatag cgagcaaatg tgaataatct atgaatgatt
    49201 tcctataagg aagtaaagat ttttctgatt ttctttaaat gtagtcttgc attttaaaat
    49261 tcttagtgct actattatta agtacttttt gtacgggccg ttagctcagt tggtagagca
    49321 gct
//
LOCUS       DAYYNQ010000008        45422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000008 DAYYNQ010000000
VERSION     DAYYNQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.008"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..719
                     /locus_tag="ACHBJ1_000454"
     CDS             <1..719
                     /locus_tag="ACHBJ1_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429427.1"
                     /translation="DQAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVY
                     QTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFI
                     GTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILA
                     VGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGF
                     VLLMITNTIARRFDSDSALF"
     gene            731..1651
                     /locus_tag="ACHBJ1_000455"
     CDS             731..1651
                     /locus_tag="ACHBJ1_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429428.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1692..3149
                     /locus_tag="ACHBJ1_000456"
     CDS             1692..3149
                     /locus_tag="ACHBJ1_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9429429.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3494..4108
                     /locus_tag="ACHBJ1_000457"
     CDS             3494..4108
                     /locus_tag="ACHBJ1_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9429430.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4101..5825
                     /locus_tag="ACHBJ1_000458"
     CDS             4101..5825
                     /locus_tag="ACHBJ1_000458"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9429431.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5822..7273
                     /locus_tag="ACHBJ1_000459"
     CDS             5822..7273
                     /locus_tag="ACHBJ1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9429432.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7746..9899)
                     /locus_tag="ACHBJ1_000460"
     CDS             complement(7746..9899)
                     /locus_tag="ACHBJ1_000460"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM9429433.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9901..10947)
                     /locus_tag="ACHBJ1_000461"
     CDS             complement(9901..10947)
                     /locus_tag="ACHBJ1_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9429434.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11097..12386
                     /locus_tag="ACHBJ1_000462"
     CDS             11097..12386
                     /locus_tag="ACHBJ1_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM9429435.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12399..15101
                     /locus_tag="ACHBJ1_000463"
     CDS             12399..15101
                     /locus_tag="ACHBJ1_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM9429436.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15086..15943
                     /locus_tag="ACHBJ1_000464"
     CDS             15086..15943
                     /locus_tag="ACHBJ1_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9429437.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(16014..16871)
                     /locus_tag="ACHBJ1_000465"
     CDS             complement(16014..16871)
                     /locus_tag="ACHBJ1_000465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9429438.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17082..19253
                     /locus_tag="ACHBJ1_000466"
     CDS             17082..19253
                     /locus_tag="ACHBJ1_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9429439.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19321..19662
                     /locus_tag="ACHBJ1_000467"
     CDS             19321..19662
                     /locus_tag="ACHBJ1_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9429440.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19938..21692)
                     /locus_tag="ACHBJ1_000468"
     CDS             complement(19938..21692)
                     /locus_tag="ACHBJ1_000468"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429441.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21703..22539)
                     /locus_tag="ACHBJ1_000469"
     CDS             complement(21703..22539)
                     /locus_tag="ACHBJ1_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9429442.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22541..23224)
                     /locus_tag="ACHBJ1_000470"
     CDS             complement(22541..23224)
                     /locus_tag="ACHBJ1_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM9429443.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23383..24777)
                     /gene="uxaC"
                     /locus_tag="ACHBJ1_000471"
     CDS             complement(23383..24777)
                     /gene="uxaC"
                     /locus_tag="ACHBJ1_000471"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM9429444.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24816..26315)
                     /locus_tag="ACHBJ1_000472"
     CDS             complement(24816..26315)
                     /locus_tag="ACHBJ1_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM9429445.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26308..27729)
                     /locus_tag="ACHBJ1_000473"
     CDS             complement(26308..27729)
                     /locus_tag="ACHBJ1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM9429446.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27789..28781)
                     /locus_tag="ACHBJ1_000474"
     CDS             complement(27789..28781)
                     /locus_tag="ACHBJ1_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9429447.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            29013..31211
                     /locus_tag="ACHBJ1_000475"
     CDS             29013..31211
                     /locus_tag="ACHBJ1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429448.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31669..32541)
                     /locus_tag="ACHBJ1_000476"
     CDS             complement(31669..32541)
                     /locus_tag="ACHBJ1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429449.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            32841..33815
                     /locus_tag="ACHBJ1_000477"
     CDS             32841..33815
                     /locus_tag="ACHBJ1_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429450.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            33837..34529
                     /locus_tag="ACHBJ1_000478"
     CDS             33837..34529
                     /locus_tag="ACHBJ1_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9429451.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            34495..36003
                     /locus_tag="ACHBJ1_000479"
     CDS             34495..36003
                     /locus_tag="ACHBJ1_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9429452.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            36077..37180
                     /locus_tag="ACHBJ1_000480"
     CDS             36077..37180
                     /locus_tag="ACHBJ1_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9429453.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            37183..38520
                     /locus_tag="ACHBJ1_000481"
     CDS             37183..38520
                     /locus_tag="ACHBJ1_000481"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM9429454.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            38507..39385
                     /locus_tag="ACHBJ1_000482"
     CDS             38507..39385
                     /locus_tag="ACHBJ1_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM9429455.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            39382..40305
                     /locus_tag="ACHBJ1_000483"
     CDS             39382..40305
                     /locus_tag="ACHBJ1_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM9429456.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            40302..40712
                     /locus_tag="ACHBJ1_000484"
     CDS             40302..40712
                     /locus_tag="ACHBJ1_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM9429457.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            40716..42284
                     /locus_tag="ACHBJ1_000485"
     CDS             40716..42284
                     /locus_tag="ACHBJ1_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM9429458.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            42524..43942
                     /locus_tag="ACHBJ1_000486"
     CDS             42524..43942
                     /locus_tag="ACHBJ1_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429459.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            43954..45264
                     /locus_tag="ACHBJ1_000487"
     CDS             43954..45264
                     /locus_tag="ACHBJ1_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM9429460.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        14726 a         8002 c         9292 g        13402 t
ORIGIN      
        1 cggatcaagc atttctcatc actaaaaata caattttata taatgtagca tttatccttt
       61 tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga aataaacgac
      121 tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg gtcattatca
      181 gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag tggatcactg
      241 gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg tttatcttgg
      301 tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt gcatctgtta
      361 tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc aaatggcagc
      421 agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta acgattttag
      481 ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct aggaattccg
      541 gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc ctgacatcca
      601 caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc ggctttgtct
      661 tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca ttattctaag
      721 gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt tcaataagac
      781 ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta ttttgccatt
      841 tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc acgggtacag
      901 tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc agatgcagga
      961 taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta cactgatcaa
     1021 tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc cattccgtcg
     1081 tttttttacc gttttttgtt taattacgat gttgttctca ccagggatgg tggccaatta
     1141 tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga tcttgccaat
     1201 ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt cggtctctga
     1261 ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat
     1321 cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg cattgggtta
     1381 ctggaatgac tggttcaatg ctttactata catccaagat gataagctag ttccactaca
     1441 gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt caggagcagg
     1501 aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa tggcgatcgt
     1561 agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc attttgtcaa
     1621 aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc ataaaattag
     1681 gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca gcgttgttgt
     1741 tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa gctagcaatt
     1801 cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat caattgatgg
     1861 aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc aattacattg
     1921 gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa aattatgata
     1981 tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct gatttgactg
     2041 aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca tatattaaag
     2101 gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac
     2161 aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt gatggaatcc
     2221 aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg
     2281 cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat cctttaggga
     2341 atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc aaccaatacg
     2401 ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa caaggactca
     2461 ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat acttggctaa
     2521 tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca aatgcagcaa
     2581 aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct caagcacaaa
     2641 tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta gaacttctag
     2701 ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc gagggagaag
     2761 catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt tatcagccaa
     2821 atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca caagagtcta
     2881 ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa acttcaccga
     2941 ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat atttctaatg
     3001 tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat gaaacattac
     3061 cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa
     3121 aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg ttgtgacaaa
     3181 agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt tctcggtatt
     3241 aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct tactactcga
     3301 aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta accctcggta
     3361 ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct ctaataatca
     3421 ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa acttaatgtt
     3481 tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga tctgggtcat
     3541 tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca tctttggatt
     3601 aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg agtataaaga
     3661 aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag gcaatctgct
     3721 atttagttta tttttatctg gtactgcttt attgagttac aatttatatt tatctgttca
     3781 aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct tgctctgcct
     3841 atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct ctgttcctaa
     3901 tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc taaaaataat
     3961 tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga tcctgtttgg
     4021 tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc gtgaagaatt
     4081 agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa ggaaccttac
     4141 taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg actgctaccg
     4201 ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg
     4261 atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt aggatcatca
     4321 aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac
     4381 ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat gaattagttt
     4441 ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt gcgatatata
     4501 tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa ggtggggaaa
     4561 taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct atcacacaag
     4621 gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta gatatgattt
     4681 tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga acgacaaatc
     4741 gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga caagaaatga
     4801 tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg gaaaaaaaca
     4861 attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta ctagataaaa
     4921 aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt atcattattt
     4981 tagacaatat actgcttatc tttctttata aaacattcaa acgatattca cagcaagtat
     5041 ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg cggatcgaca
     5101 taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa atgttggata
     5161 gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa gacgcacata
     5221 tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta gaatatatcc
     5281 gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat gcgttttccg
     5341 ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa gaactggact
     5401 tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt
     5461 ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc cagccattag
     5521 ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg ctaagtgtca
     5581 aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga aaagggatga
     5641 ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg gaacttcacg
     5701 gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc agcagttatt
     5761 atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca ttcaaggagg
     5821 gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga atgggttaaa
     5881 aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag agaatgccaa
     5941 agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg atgtgacaat
     6001 gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc ataattttga
     6061 atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta tgcaattagg
     6121 cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa
     6181 aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa tctatcaaga
     6241 actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag aagaaataat
     6301 aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa gaactcatga
     6361 tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc aacgaaatta
     6421 cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg gcttctgttg
     6481 ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg aagagacaga
     6541 ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac agttgcatca
     6601 attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa caaaagatca
     6661 aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca
     6721 aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc cggaagatat
     6781 acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg atctggaaga
     6841 tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca
     6901 gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt
     6961 ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac tcaaagaagt
     7021 gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa
     7081 aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac aactgctttt
     7141 gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca acaccacgta
     7201 tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc gagaaaacag
     7261 tggagaatca taaaaaaaaa agccaatgta atgaaataac ttataaaaaa ttgtctagaa
     7321 atctaatttt gggctgttct tattacttga ggcaaggcag ctttttcatg acctctcgat
     7381 aaacggagtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa
     7441 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca cgacctctcc
     7501 actaaatggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc aaaatatgag
     7561 gaactatttt gccatttgtc tctctaatac tcaggtcaga acgctgattt cacaacctct
     7621 caataaacgg tgttcaaaag cttgcatctg cggtaataag accaaccaaa cgaaaaatat
     7681 gaggatctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt tttcacaacc
     7741 tcttgttaat tatagtttga taaggaaaaa ggcaaatctt ttgcttgata tttcttaggg
     7801 tgagggatga tccctaattg gtaagagatc gtgccgcctt tcagcaaatc ttgatgggta
     7861 aaatataatt tcgtatattc tctgtccatc cgtttgattt tatggataaa ttgttgctgc
     7921 ggccgattag gcgttgcttc aatcgtcaat tcctttccat ttgacaaaag caaaacagcc
     7981 ttgtcaatta atggcatacc gataacatat tcaccggttc caggcgtgac aggataaaat
     8041 cccagactat tgaagatata ccaagcggcc atacttccat tgtcctcatc tccagggtaa
     8101 cccattggag aatcgtcgaa ggtctgagtc atcaactgtt taagcagtgg ctgtgccatt
     8161 tcaggttgtc ctatataact gaacaagtaa ggatagtgaa aacttggctg gtttgaaata
     8221 gctaattgtc caaattctat cgcggccatc tcactcattt cgtggatctc aaaaccatat
     8281 cctcctgggt caaacgttgg gggctgattg cacagctcaa tgagtttttc tttatatctc
     8341 tcactgccac catattttcc gatcagtcct gaaaaatcgt gataaacagc aaagctgctt
     8401 tgccaagcac ttccttctgc ataatcttgt ccccaacgat tagctgaaaa tggttctctg
     8461 aacttgccat ctttatcttt tgaacgcata aatcctgact catagtcaaa gatattttga
     8521 tagttcaatg attgctgctg atatttcttc gtcatttcct cttcttctag taccttggca
     8581 acttggctaa tacaaaaatc gctataagca tagtctaatg tgtgattgac tgattcgtgg
     8641 tagtgattag gaacatagcc atatgtcaag taatcttctg ttccttggcg tccataattt
     8701 ggatgtttgc tttgaaccgt agcacctttt tccattgccg ttagaaattc aggcatcaag
     8761 tctcgtcgga tatcttttac agcagcatct gcaatgaccg catcgatcaa tgtcccaggc
     8821 ataagtccac gttcatcagg agacagccat tttggcaaat agccactttc tctatagctg
     8881 ttcaaaaaac cttccagcat ttcttcgtat ttttctacag caatcaaaga ataaagagga
     8941 taaaccgttt tatacgtatc ccaaaaccca ttatttgtat agagaacacc aggttttata
     9001 gatcgactag tcgtatcata gtggattttt tgcccactag gatcaatttc ataaaatgtt
     9061 tgtggaaata aaaacacccg atataaatta tgataaaacg tccgcaattg ttgttgatca
     9121 tggtgtgtga gctgtatacg atcaaaatag gactgccatt gttcttgact gtgagcaagg
     9181 taagtctctt tgctttggtt ttgttctagt tccaggttat aagctgcttg ttccgagctg
     9241 ataaaagatg tcccaaatcg gatagtttgt ttttttgtct gttcaaaata caacgggatc
     9301 gagtcgctat ttttgttgga aacagctaac tcatcaattg ttaaaggata ttcaaatctc
     9361 aatataaaat aaaaagaaaa atcaggatct tctgaaccag caaaatgtgt gatttttcct
     9421 tccactgtgt aaggatctat aacaaagagc tgatggcgtc ccggaaagga taaaagtaaa
     9481 ccgctgtcat ttttgctgta atcaatagat aagattccgc catacatgct aggtatcaat
     9541 gtagaacaga tgccataacg taattgacag atcgacagat gagtcgggca aaatatactt
     9601 tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt tctcctgtag ttttccattg
     9661 atcggcgtaa acagcatatg actgaaatca cccatccaag gacttggttg atgggtaaga
     9721 cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc agctgccgtt ttgatccgta
     9781 gtttgcggtg caaaataatt catcccaaaa ggaacgccag tataaggctg gcagttccca
     9841 ttagagaaat ttggctgatt cgctgttgca tgtctagtat ctatttgacc gatattcatt
     9901 ttatttctcc tttgttgatt gtcttttgat gaatgaaact ggaaatttac tatcggatgg
     9961 tcgatctttt gtctctaacc aagtgaccaa agcatctccc gcaagcttac ctaactgttc
    10021 aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga gctgctgctt ggatatcatc
    10081 aaatccgata attgaaaaat cttcagggat atgataacca tgctgtctca agagattcat
    10141 ggtctggata gcgacaacat cgttttcaca aacaaaagca gttacttcat acttagtgat
    10201 ataagaaagc aattcatcta atgtcgcgct tttgtcatcc agtgaagtgt ggaaagtcaa
    10261 accagctttg tccaatgctt caaggtaacc aaggtaacgc tgtcgaacgg attgtggatg
    10321 agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc tgttcgatca aataagaggc
    10381 agcaagcgca ccgccttgga aattatcgga aagaacggta ggaaaaggta actcatagat
    10441 ttttttatct aatatgacga cagggaattc tttcaatgaa agctcaaaaa gtaagtcaag
    10501 atgttgttct gtatcaagag cataataaat caatccgtca aattcattga ttagctcttt
    10561 tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg acatcaatct gtttatcttg
    10621 catcacaggg ttcaatccct tgctaaaatc accaacagac aaatcagcga catatggaag
    10681 aagaaacaga atccgtcgtg tttttgtagg agaagcaaga atctgttccg gagaagcgga
    10741 tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt cctaattgct ctaattcagt
    10801 caatgcacgt ttggaagtga ttctgcttac tttataggta cgtgaaagtt ctttttcagt
    10861 tggtagttgg ctgtctacag gtaattcacc agttaaaata gccgtttgca aatcattaaa
    10921 tattgtctgg tacaatggtt gagtcatata aacacctctt aatgatatat catattcaaa
    10981 taataccata atggatgaaa aagtaaataa aaagatattt tttgaaagcg tagacataat
    11041 tggctgtatg aagtataatc tttaaagata tatcaaatag aaagaaggtt ttattagtgg
    11101 tttataaaga tataccaaaa tcggtgcaga aatttatgga agatatcaca caaaaatgtg
    11161 gggatatcca tgcggactgg gcaaagaatt ttacagcagc atttgccaat actctgttga
    11221 caacagtcaa aaagcaaaat gacggaacca cctttttatt gacgggagac attccagcga
    11281 tgtggttaag agactctact gcacaagttc gtccttattt agtgattgca aaagaagatg
    11341 aggaattagc tgacatgatc agcgggttag tcaaaaagca atttttttat atcaatatcg
    11401 atccatatgc gaatgccttc aacgaagaag ctaacggtgc tggccaccaa acagatcata
    11461 caaaaatgaa cgattggatc tgggaaagaa aatatgaaat tgattcttta tgctacccaa
    11521 tacagttggc ctatcttctt tacaaaaata caggaaaaac tgatcaattc aatcaatctt
    11581 ttattgaagg tatcaaaaaa atcttggacg tgttcaaaac agagcaagat catgaacact
    11641 ctccttatac atttgtacga gacacgacaa gaaaagaaga tacgctgatc aatgacgggc
    11701 gaggtgcaaa aacagctttt acaggaatga catggtctgg tttccgtcct agcgatgatg
    11761 cttgccgtta tggatatctg gtaccatcca atatgtttgc ggtggtcgtg ttaggttatg
    11821 tggaagaaat ttttcaaacg atactcgatg ataaagaaat tgtacagcaa acacagaaat
    11881 tgaagaaaga gatccaagag gggattcaga atcacggacg aacggttaat caaaaagggc
    11941 aaacgattta tgcatatgaa gtagatggaa tgggaaacgc cacagtcatg gatgacagta
    12001 atgtgcccaa tctgatcgcc gcaccttact tgggatattg ttctgaacaa gaggaagatt
    12061 atctgcaaac aagaagaaca ttgttgagta aagaaaatcc atatttctat gaaggaaagt
    12121 acgcaaaagg aattggatcc tcccatacgc cagaaaaata cgtttggccg attgcattag
    12181 ctatggaagg aatgacaacg tcagacaaat ctgaaaaaga gcggatattg aatttacttg
    12241 tagcaactga cgcaggaaca caccttatgc acgaaggatt cgatgtgaac aatcctgaaa
    12301 attatacgag agaatggttt tcttgggcaa atatgatgtt ttgcgaatta gtcatggatt
    12361 actttgatat tagagtagaa aagtaggagg tatcgtaaat gaaaaagaaa gtatatatca
    12421 tttctcatag ccactgggat cgtgaatggt acatggctta cgaacagcat cacatgcgac
    12481 tgattgaatt gatagacgat ctactagagc tatttgaagt tgatccgtcc ttcaacagtt
    12541 ttcatttgga tgggcaaacg attattttag atgactatct tcaagtaagg ccagaaaaaa
    12601 gacaggcaat ccaacaagca atcaatgaag gaaaattgag aattggacca ttttatatct
    12661 tgcaagatga ttttcttatc agctcggaat cgaacgtgag aaatatgctg attggcatgg
    12721 aagaaagccg taaatgggga acccctgtca tgttaggcta ttttcccgat acatttggaa
    12781 atatgggaca gacgcctcaa ttaatgaaac aagctggtat ttcagcagca gcgtttggcc
    12841 gtggtgtaaa accaattggc tttgataatc aagttttgga agcagaaaac tattcttccc
    12901 aatactcaga gatgtggtgg aaaggccctg atcaaacagc aatctttggt ctgctatttg
    12961 ccaattggta tagcaacggg aatgagattc cagtagaaaa agaagcagct cttgcctttt
    13021 ggaagcaaaa attagcggat gcagaacaat atgcctctac gaaccactta ttgatgatga
    13081 atggtgttga tcatcaaccg gtacaaaaag atatatcaaa agcaatccat ctcgcaaatg
    13141 aactgtttcc agattacgag tttattcatt ctaattttac tgactatctg gaagcagttc
    13201 aaaaagatgt gccagaagac ttaggctcag tagaaggaga attgacaagt caagaaacgg
    13261 atggctggta tactttagcc aatacagcat ctgctcgggt ttacttaaaa caatggaata
    13321 ccaaggtcca gcgacagcta gaaaatatta cagaaccact tgcgacgatg gcttatgaag
    13381 ttagtggaaa ctatccccat gatcaattag attatgcttg gaagacatta atgcaaaatc
    13441 atcctcacga cagtatttgc ggttgttcag tcgattctgt ccatcgggaa atgatacctc
    13501 gttttgaaaa agcagatgaa gttggaaagt atcttgcaca agattcctta gaacagctga
    13561 ccgcagcaat cgatacgacc ggattcccaa aagacagctt tccatttgtc attgtaaata
    13621 ctgcaggaat ggataaaact ggtgaagcag aaataaccat cgaattagaa agaaaaagat
    13681 tcgctgaagg aatacctgaa caactatatc aagagttaga aaatttgcct aaacgtaagt
    13741 atcatgtaga aacaaaaagt ggggccacta taccagctat tttatctgaa gaaactgtcc
    13801 aatttggtta tgatttgccg aaagaccgtt tccgtgtgcc atatatggca agaatgatca
    13861 aagtgacttt accattagaa aacatgccag cattttcttg ggaaacattc gcgcttgtag
    13921 aaggggaagc gaaagcagaa gaaaaggaaa caatgattca tcagagtgga cgaatcatcg
    13981 aaaacggccc tctacatttg acgattgaaa aaaatggtac cattacaatg gaagatcgca
    14041 aaaacaaacg aaagttgaat aatctgcata tttttgaaga tatcggcgat ataggaaatg
    14101 agtatatctt taaacaacct ttctgtgatc agccaatcct atccagtaac aaagaaaact
    14161 ctgaagtaaa agtattagta gatacaccgg aaatagcaaa aataagtatt ctacaggaaa
    14221 tggaaatccc tgtttctgca gacgagcgtt tagaaaaaga acagcagatg gttgtggaat
    14281 ttcgttatcg aaaagcagaa cgatccaaag aaaaacgaat ccttcaaata aaaacaatca
    14341 tgacaatcag aaaagatagt aaaaaaatcg actttgaaac gactctcgac aatcagatga
    14401 aagatcatcg cttacgtgtc ttgtttccta caaagttaca tgtggaacat cacgaagcag
    14461 atagtatttt tgaagtggtg aagcgcccaa accatgtgtc taaaagttgg gaaaacccca
    14521 cgaatccgca acatcagcaa gcatttgtca atattcatga tgaagagtac ggtgtaacag
    14581 tcggaaactt tggtttgaat gaatatgaag taacagaaga cggacaaatc gcagtgacgc
    14641 tacttcgcag tgtgggagaa ctaggcgatt ggggatattt tccaacacca gaagcacagt
    14701 gtctaggtga gcatcggttc aattacagca ttgaactaca tggaccagaa gaaaaattct
    14761 ctacttattt gcatgcatac gcagcacaga ttcctttttc cactcaacaa atcaaacatc
    14821 atgaaggaac attgatttca aaacaacaat atttaacgat aaaaagtgaa acgtttgcaa
    14881 ttacagcttt gaaacgcagc aagtttagtg ataaagtagt agtaagaggt ttcaatatgt
    14941 ctagtcattt agaaaaattg gaaataacta aagacaatgg aaaaactgtc atattgaatc
    15001 tgcttgaaga accaacgaag caagcggttg tcccaataat ccaaccttat gagattcgga
    15061 caatcggatt cgaggaggaa aacgaatgta tggcggaata gaagcaggcg gaacaaaatt
    15121 tgtgtgtgcg gtttcagatg gtggagaaat tttagaaaaa ttaagtatcc ctaccacaac
    15181 accagaagaa acattggatc aggtatttga attttttgat cgttatgaat tgaagggaat
    15241 cgggattggc tcattcggcc cgattggtat cgacaaaaca aaagataatt acggatacgt
    15301 tttggcaact cccaaaaaag ggtgggcaca attcgatttt cttggcacaa tcaaggaacg
    15361 ctacaatatt cctgctgctt ggacaacgga tgtcaatgct gctgcatatg gggaattgaa
    15421 gcaaggagca gcacaaggaa aaaatagttg tatttacctt accgtgggaa caggaatcgg
    15481 tgcaggtgtc gtgatcaatg aagaaatttt ttcagggatc gcacatcctg aaatgggaca
    15541 tatttgggtg aaacgccatc cagatgacca ttatgaaggt acttgtcctt atcataaaga
    15601 ttgtttagaa ggattagcag caggaccgtc tattgaggca cgcacaggga tcaaagggca
    15661 agatctgccg caagatcatc ctgtttgggc tattcaggct tattatattg ctcaagcact
    15721 gatcaactat acgttgacgt tagcacctga aaagattatt ttaggcggtg gggtaatgaa
    15781 tcaggatcat ctccttcaaa aaatccgtca gcaatttgtt gaattgatgg gtggttatat
    15841 ggaaacacct caagtaagtg aatatatcgt tcgttgggga ctgccgaatg aaagtgggat
    15901 tatcggtagt ttgctgttag cagaacacga acatcaaaag taagcagaca aattacacaa
    15961 aaagaacaga gtcttatcat tatctgctga taagactctg ttctttttta tttctattcc
    16021 cataatccag gtaaagcttt accgactatt ttttcttgag taaatggatg gatcatgtgc
    16081 agttctaatg catggagcat cagtcgttca gctgttctat tttggtagag agggtctcca
    16141 acgatcggat gtcccttatg agctaaatga actctgatct ggtgtgtacg tccggtttcc
    16201 aaaacacaat agacttgtgt ttgttcagaa agtactttgt tgacagtgac atgtgtcaag
    16261 gcgctctgac catttttatc atctacgacc ctttttctcc gatcatggcg atctcgtcca
    16321 attttattgg tgatggtcat atctttggca atttctcccc atacgatggc ttgatatctc
    16381 cgatagatca ttttttgttc caacattctt ccaagtatcg gcaagacaaa tggatttttt
    16441 gcaaataaaa cggcaccgct tgtttctttg tccaagcggt ggacaacgta aggtcgttgc
    16501 tgtttagtgt cgagataagc agctaaatga ttgagtaagg tactgttttc tgtcggttca
    16561 tttggatgag tcttcattcc tgcaggctta tccactataa tcaaatgctc atcttcatat
    16621 aaaacacgga tgtgcttcgc atctcctaat tgaacttctt gataattata atctgtttct
    16681 tcgaataaca atgtgatctg gtcattgccc tttacttcat gatggaacaa agcaggttgt
    16741 tgattgatcc aaacgttttt acgtatacgc aaaaagtgtc gaacctttct aggaactaac
    16801 cattcctttt caagcaagtc gcgaattgtt gtttcaggat ggttcatagg taaagtgatc
    16861 gtgattttca caagtgtact cctttcagtg caatcgcaga ctaattgtac catttttaaa
    16921 acatatttaa gaaaaaactt acatttttct gttcattctt atgttactct acttgcacta
    16981 acgtaaaaaa acatattgaa ctacaactta agcaggaaga aaacactctt tcactatggt
    17041 aaaataagac gattgaatca aatagacagg agaagaagca aatggatttt caaagcattt
    17101 tagaaaagat aaaagctgct ttgatcagtt tttggcgttg gatcagacca tatctcggac
    17161 aattccatcg atggcgaaag cgcatctgga aaaaatatca tgtgaacaaa ttgattttgc
    17221 tgattggttt gatctgtgtc ttagtgacaa gtatctacct ctttatttta gcaaaacaag
    17281 caaatgtcga aacgttaaaa tcaggattaa gccaatcgac agtcatttat gataagaaaa
    17341 acgaagaagc tggcacactg tacgcccaaa aaggaacata tgtagaatta aacgcgattt
    17401 ctccctatat ccaagatgca gtaatctcga cagaagatag gaatttttac gagcatcacg
    17461 ggtttgatat caaagggatt gcccgtgcag cagtccgaat ggtcttgtct ggcggaacat
    17521 cagggggcgg aggaagtacg atcactcagc agcttgccaa aaatgcgtat ctgacattag
    17581 accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc aatcgagatc gaaaagaagt
    17641 acagcaaaga agaaatcttg accatgtatc ttaacaatgc ctattttggc aacggggtat
    17701 ggggtgtcca agacgcttcc agaaagtatt ttggagtaga tgcaacgaat gttacgctgt
    17761 ctgaggctgc tactttggca ggaatgctga aaggtcctgg tatttataat ccgatcgatc
    17821 acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag tgtaatggaa gaaaacggaa
    17881 aaatcacaaa agaacaagaa caagcagaaa gccagacaga tatcagcgcg tatcttaatg
    17941 acacatacga taattcagat tctggttatc gttatcctta ttattttgat gcagtgatcg
    18001 atgaagcagt taacgagtat ggtcttgatg aagaagatgt catgaataaa ggctataaaa
    18061 tctatacagc tttagatcag gattatcaaa agcaattaga agctacttat caaaacagtg
    18121 cattattccc agcaaatgct tcagatggtg ccatggtcca atctgcttca gtagctttag
    18181 atccgaatac aggtggtgta caagcactag taggacgaag aggagatcat gtctttagag
    18241 gattcagctt tgctacacag atgcgtcgtt caccaggatc aacgatcaaa ccattaagcg
    18301 tatatacacc agcactggaa gctggataca agccttccag tatcttgaaa gacgaaccgc
    18361 aaagttatta cgatgcgcat aattttgacg ggacatacca aggagaagta ccgatgtatc
    18421 aagctgttgc acaaagtttg aatttgccta ctgtatggct tttgcatgag atcggtttac
    18481 aaaaaggata tgataaagca gaagaattcg gcctgccttt agcaaaatct gataagtatt
    18541 atggcttagc ccttggtgga ctggaaaaag gagtatctcc attgatcatg gcgggtgctt
    18601 acagtgcatt tgccaatgat ggtcagatgt atacgccgca tcttatcacg aaaatcgttg
    18661 attcgactgg agcggtgatc gtcgataaca caaacaaaaa accaaaacag gtcatatcaa
    18721 aggaaacagc cgatgaaatg accagcatgc ttcttggtac attttcaaat ggaacagcgg
    18781 ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac aggaaccaca gaaactaatt
    18841 tcgatgccac aaaagcaaat gaccaatgga tgatcggtta cacgccagac gtagtgattt
    18901 cgacatggat gggttacgaa gaatccagca agctgcacta tttggaaggg acaagtggaa
    18961 atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat ccttccgtat acttcaaaaa
    19021 caggatttag tgtagcagat gcctatgcca caggaggaca agtggtagct gcagatcaag
    19081 ttggaaacac aggaaattct aatggagagt ctggaaattg gcaagataac ttgaatcaat
    19141 atggtgaaca agcaaaagaa gggctgaaaa acttcggcga tatggtgaaa gaaggcgttc
    19201 agggaattgg tgaagcggct aaagacctct ggcggaaata tcaaggagag tagccccttt
    19261 gaaaacgaat aaccccatgt tacaatagag agtaactact aaaagaaaag agggcataac
    19321 atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg agatccgtga attaccagaa
    19381 ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa acgaagaagc acacaaactg
    19441 ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa aacaaatgag tggacaagag
    19501 ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg tgaaaatcca atcagaagaa
    19561 gtaatcaatg acttgatgca aaaagagcaa ggcttcagta caatcatcaa tgacttgaac
    19621 cgtatcatca tgacaccagt tcgcgacctt tacagcgaat aaaactattt tatgaaacaa
    19681 cagagggtgt gaaagagacg ttcggccttg agcgatgaca aaattgcttc ttattgccga
    19741 taggctgtct cttgaacacc atttaccaag aggtcgtgag cctaccgttt aactctgagt
    19801 tacaaggaac aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc
    19861 gaggagttgg caggagaaca ccgtttattc agaaatagga gctacgacaa aagtcttgtc
    19921 atagctccta ttttttgtca ttcatttttc aagcggtaga aatagccttt ttgagctaac
    19981 agtttttcgt aagtacctat ttcaacgatt tttccttcat cgatcacaac gattttatct
    20041 gcctctttga tcgttgaaag ccgatgagcc acaataaaag tagtgggtgt ttctgtcagg
    20101 taattcaggg cttgctggat ttttttctct gactggttat ctaaagctga ggttgcttca
    20161 tctagcaaaa tgatttttgg ctgacgtatc aacgctcgtg caatggagat acgttgcttt
    20221 tggccaccag acagcttgtt gccatgttcg cctactaagg tatccagacc atcaggaagc
    20281 tggtcaatca gtgaagaaag ttgcgcagct tcgatgactt catctaaacg ttcttttgat
    20341 acgtttttca agccataagt gatgttctct ttgatcgaag cggagaacaa caaagtggtt
    20401 tgaggaacaa cggctaaata atttcgcaca cttcgcatat ccagttcatc caaaggctga
    20461 tcatctagaa aaatttttcc tgaagtcggc tggataaatc caatcaataa gttaaggagt
    20521 gtagattttc cagagccaga tggcccaaca aaagcaattg tttctccagc tttcacctct
    20581 agagaaaaat gatcgataat cggtttgagt gagtccggat aaagaaaaga aacatcctca
    20641 aaacgaaaat ttccatccaa atgttggatt tttttcttac cgtgataagt ttctacatcg
    20701 ccagcaatca acagttcgcc aatagaagtg atagattcca tgccttttga aatcgtagga
    20761 atcagcgtga tcattccaga aacggaattg acgatcgtcg tgaaatacgt ctgatacatc
    20821 acgacatcgc cgggttggat gcgcccttgt aaagctaata tcccagtgaa aatcaaacaa
    20881 atcagctgaa atatctgaaa actagcccag ctgactgatc cgaaaacaga ttgcaggata
    20941 tctaagtgga atcctttttc atagatttga gtaaaatgct tatttaagcg agttgtttcg
    21001 tgtttttcta acccatgagc tcgagcaatc ggtgctaatt cgatcacttc gatcactttt
    21061 gcagaagtct cttccatttc ttgcctgaag tctgagttgg actgtctgat ccttttacgg
    21121 aaaaagagag taacgaatgc ggcgagagga acagtcaata agaaaaacat gaaaacaaca
    21181 ggacttttat aaagcgtgat acctaaagct acgaataaat tgacagcgat atttaatccg
    21241 ctgacaaata cttgagagga caacgtttgg atagcttcta catcacggat gatctttgac
    21301 tggattcgtc cagattgcaa ttctttttga taaggaatag atagttcttg gattttcttg
    21361 ataagggtcg ctcttagtcc agcttctact tgacggatcg aagtgctatg gtattttaca
    21421 tgaaaataat tcgtcaatat attttgacca atcagacaag ccatcaaaaa agcgttgaaa
    21481 taaatggcct gcaactgaga agaatctttc gatgaagctg cattgattac attggcaata
    21541 gcgatcggca ttaaccaaga tggaagatgt ttgataataa aaaagagtcc tgacatggca
    21601 aaacgccaat aatttccctg ataaagatgc agcaatgtct gaaaagcatt tgcatgatat
    21661 ttttgataac ttttctgtat tcgttcctcc ataatcttct ccttaacaat cttggtaaat
    21721 aaactgcaag tgttctattg cttcaggaac ataaatctca ggagtagctt caagagtggc
    21781 gtacattaga ggacgctcat atttgatata atgcagtacc gggcggaaat ctaaacagcc
    21841 cttgccaaca ggtaccatgc ggatctttcc attttccatg atatagtctt tgagatgaag
    21901 cgctgtaata taaccatgta aattgtccaa ggcacctcgg atcacttttt cctgatcagc
    21961 atgattttct ttatgcatca aattcgcaca atctaaaatg atttttaaat ttggtgaagc
    22021 tacttcatcg attaaacgtt tagctaactg atagttatag attggatgat tgatccctgc
    22081 ttcaacagca acagttacgc caaaattttc tgcatgagcg actaattcaa tgatagaatt
    22141 tctggcgatc tgataagctt cttcagtaaa attttctttt gtgtaaccga cagagacact
    22201 accggtttcc gtagcaatca tcttggcttg ataggcagag cacagagcaa tttgctgttt
    22261 gaaagttgcc aatgcttgtg cacgaacttc ttgatttttg ctggataggt tgatatagca
    22321 tcctaaaatg gaaagttcga tattgttttt ttggaagtat tcaccaaaat aggcagcact
    22381 cccaagtgtc atgtcagtta atttaagttg ttcaggaaat gctttttgtg gtgcaaattg
    22441 gatatgggac aagtgaagct gtgatacttt ttgaacgatt tcttcacgat ttcgaacatc
    22501 taaatcgtgg gcacggatgc ctaaatttaa tggaaacatc tcaaactcct ttttgtaaat
    22561 aattagcaag aggcagacgg gattctttta tcaattcaac aattattcgg gcaactttat
    22621 cagcgccttc tggactaaag tgggtattgt cttttagtcc ttctgggtaa tttttacaag
    22681 tatcaggtat aagatgcagg tagaattttt ttgtctcttc ttttgagaag gtctggaaca
    22741 attcttgtga tcttgagaac acatctaaaa gaggaatatg gtgtttctca gcaaatgcac
    22801 gcatcgcttt tggatagtct ccaagtatat ctggattcaa tgttccgttt tctaagtaat
    22861 ctcgtcgcgt cacagatgtc agtaagacca tttggacgtt cgcttttttt gcgcattcaa
    22921 aaaatatctg cagatttttt aaatagctac cgaagggaag cgtacctctt tcttcttgga
    22981 ttttctggtc gttatgtcca aattgtacaa ataagaaatc attcggctga aactgtgaag
    23041 ctagacgggc taaccttcct tcctggataa atgatttggt gctgcgtcca ttctttgctt
    23101 ggttattgat ctggatctcg ggtgaaaaat aagcagagat tttttctccc caacctgtct
    23161 cgggacgctt gttttcatct ttttctgccg cagtagaatc accggcaata tggatagtag
    23221 gcatgttatc tcttctttca taaaggttta taggcaatga agcagaacag caagagaaga
    23281 agacggagca aatttggtcc gtcttcttct ctaaaacgtg gataaacgga ttccgttatt
    23341 tcactatcct gtttttgatt cgttactgtt actattaatc aattaggcaa aaaagccaaa
    23401 gtaattttta gcatttgtat aactaatcgc ttgaactaga cttcctaaaa gttgttcatc
    23461 ttcaggtgct tctcctcgca tgaccatttc gcctaaaaat tcacaaagaa cacgacggaa
    23521 atattcatgt ctagtgtagg acaggaaact tcttgaatct gtcagcatgc cgacaaaatt
    23581 ccccaaagca ctaccatcag caaattgtgt caattgatcg cgcataccag ctcgagtatc
    23641 gttgtaccac catccagaac ctaactgcag ttttccattc tgttcttttt ggaaacagcc
    23701 catcaaagct aaaatcgcag gatagtcatt cggattcaat gaataaagga tcgtctttgg
    23761 caactgatcc gtttcttctg cacggttcaa taatttttgc agaggaatag tcaaagaaag
    23821 atcattcatt ccatcatagc cggtatcggg ccctaatttt tccaacattt gtgtattaca
    23881 gttacgataa gcatggatgt gcagctgcat cgtccaatta tgagaatgat agaagtgggt
    23941 caaatgtatc agtgtttctg tacgataagc atcgatttca tctgaagata atagtttgtt
    24001 agctcgtgct ttttgaaaaa tctcttctaa tactgtttca tccgcttctt tatagcgtaa
    24061 gatgtctaaa gcgtggtcgg aaacacgaca tccatgttga tggaagtatt ctatccgctg
    24121 ccctaagact tctatcagtt cttgataggt ggtgatcgtt tggtcagctg cttcttccag
    24181 tagtgtgacc catgcattaa atccttcttt ttcaatattg agtgctttat ctggacggaa
    24241 tgaaggtaga accttcaaat ctggttcttc atgagcaagc aactgatgat agtgcaagtc
    24301 gtctgcggga tcatctgttg tacaaatcac ttctacattc gatttttttg cgatttcccg
    24361 acggcgaaaa tcaggagctg ccaaaatttc atttgcttgt gtccaaattt tctctgcatt
    24421 ttcttcattt atcaacaagt caataccgaa gaaacgcttc aattcaagat gtgaccaact
    24481 gtaaagcgga tttccgataa tttttggtac ggtttgacac caagctaaaa atttttcaaa
    24541 atcagaagca tttcctgtga ttttttcttc aggcacgccg caagctcgca tcaagcgcca
    24601 tttgtaatga tcgcctccta accaagcttc tgtcagattg ctgaaattgt tgttttcgta
    24661 aatttctttt ggatctaagt gacagtgata atcaataatg ggcatgtttt ttgcgtagga
    24721 atggtaaagc tttttcgccc aatcgtcttt taataaaaaa tcatcagata aaaacataaa
    24781 tcttccttct ttctttttat actgttttat gaaaatcaaa gtgttactcc gtttttaaaa
    24841 atggcaattt ctcgaacgtc attttgttca tttttcgttt cttctccact agctacagcg
    24901 atgatttttt gaacaaacga ttctaagatt acttccattg gttgttctaa taagacgcct
    24961 gcattaaaat ccatccaatg acctttgcgt tcaaaaagag ttgtattcgt agataccttg
    25021 accgttggga catatgagcc aaacggagtc cctcttcctg tggtaaacaa gaccaactgg
    25081 caatcggatg aagcaagagc agaggcagct acaagatcat tcccgggagc ttgcaataaa
    25141 ctaagccctg gttttctgat tttttcgcca tattgtagca catcaacgac aggagagctt
    25201 cctgattttt gcgtgcagcc taaagactta tcttctaatg tagtgatccc acctgcttta
    25261 ttaccaggag atgggttttc gtaaactggt tccccgtaag acaagaaata ctgtttgaag
    25321 tcattgatca agtcaacgat tgagtcaaaa acttcttcat tttccgctcg tgccatcaat
    25381 acttgttctg cgccaaacat ttctggcact tctgttaaga tcgtgctgcc tccttgagca
    25441 atcaagtagt cagaaaatgc accaagtaaa ggattagcag ttattcccga tagaccatca
    25501 gaaccgccgc atttcaaacc gacattcaac ttagagagcg ggactgatat gcgctggtca
    25561 ttttccgccg ctgttaaaag ctcttctaat agagccacac cttgttcgat ctcatcgtaa
    25621 acatcttgag ccactagaaa tttcactcgt tcttcatcat attcacctaa aatttttttg
    25681 aattcaggaa ctgtattgtt ctcacaacct aatccaaata ctaaaactcc tccggcattg
    25741 ggatgattta ctgcgtccgc taaaatttct cgagtatttt gatgatcttt tccaagctgc
    25801 gaacagccat aaggatgctt taaaatcgta atatggtcaa actgtcccag atctggatgt
    25861 ttttccttga actgtttgac gatcagttca gcaatgccat tcacacaccc aacggtaggt
    25921 actaaaaaca aatcattacg aatccctact tttccatttt ttcgcaagta accttgaaat
    25981 gttcggttct caaaaaggta atgacttgga tggacatctt tttcatatcg ataatccaat
    26041 tcaccagaaa gattggtttt tacattatga gtgtgcagcc attgaccggc agttacgtct
    26101 gtcgtcacat gaccgattgg atagccatat ttgatcacgt tgtctccagc tttcaaatca
    26161 cccaatgcaa tcttatgacc ttgcgggata tcttcaagag tggtgatcgt ttgctgatca
    26221 atcgtcagtt gtgtttgttt tggaataggg gttaatgcga cgaccacact gtctcgtcga
    26281 tttaatttca ttgttggttg cagggtttca agcatggacc atcgttcctt tcatttcttg
    26341 taatactgac cggacacctt tttgatcgat ctttgcagct atttcttgaa ctgtatctgt
    26401 cacattcggt atttgagaca aatcttctcc ccaaagcgca gcgttttgca ataatcgagg
    26461 aatagctgtt ccattatcag accaagcatc agtaaattcg cttatcgtct tttcatcatc
    26521 atgtggcttt acttgatcgc ctcgatacat aagaagtagc gcagctagtg aaacagtgat
    26581 taacggtggt acctgatttt gttcttcgat atacttcttg aacactggaa gcagacgggt
    26641 gctgaacttt gaaacactat ttaatgaaat cgcttctaat ttgtgatggg caaaaggatt
    26701 caagaaacgt tctttgattt ggtcagcata aatggctaat tcatctttag gtaaagaaag
    26761 cataggaatc aattctagat tgaataactg atcaacatat gcggagaata aaggatcttt
    26821 cattacttct tctacagttt ccaattccgc caacaaagca agagggacca tcgctgtatg
    26881 cggtccatta agcaaatgca ccttacgttc acgataagga gtcatatcat ctgtgatgat
    26941 gacattcaag ccagcctgtt tgaatggtag ttgattctcc aattctttag gcccttcgat
    27001 cacccaaagc atgaatggtt ctgctttgac catcaattga tcttcatagt tatgccgttg
    27061 attgaaaaca cctgcatctt cgcgaggata acctgggacg atgcgatcaa ctaaactaca
    27121 acagaaaaca ttttcttttt ctaaccactc aacaaaagca ggttctaaat cccatttttc
    27181 tgcgtagcgt aaaatgattt cttttagacg ttcgccgttt ctgtcaatca gttcgcaagg
    27241 aatgatggta aagccagctt tgtttaattt gaaacgtcgg tataaaaagg ctgtcagttt
    27301 gcccgggaag ctctgtgggg gagtatctgt taaattatca gtctccacga attggattcc
    27361 tgcctctgtt gtattggaga cgatataggc caagtcgtca ttttctgcta gagatagata
    27421 agcatcccat tcatcataag gattgatcat ttgttctact acggtgataa tctcggaagt
    27481 atcaacgatt tcgccattgc tgagtccttg taaaatgacc gtatataaat tatcttgctg
    27541 gttcatcata tttccaagac cttgttctaa aggttgaatg attgcaacat tacctttaaa
    27601 caattgttgg ttattcagct gctgaatctg ccaatcaata aatccacgca taaaattgcc
    27661 ttcaccaaac tgaagaactt ttacaggtgc ttcgtttttt ttcactaatt cttttgtcag
    27721 ttttttcata taaacttcct ttctgtgtta tgcacacgtt aacattttta tataaaatta
    27781 cttgtagttt acaagtaatt gatcgactca cgaaggatca acaaggtgga gagctcaatt
    27841 ttagattgtt cgatcgtcct gccttcgatc aaatctagta aaagctttgt tccttcttga
    27901 cacatttctt tcaaaggctt acgtacggtg gtaagcggcg gattgagata ggagacgact
    27961 ggagaatcat cgaacccgat cagagaaatg ttctggggaa cacggaggcc agctaaatgt
    28021 acagctctca atgcaccaat cgctatgtcg tcgttgccgc aaaaaaccag agtaggcagg
    28081 tcagaaagtg ataataggta ttccatgttt tggaggccgc tttctaagct gtaatctcct
    28141 gttaaaaaat aggctggatt gacagggatg ctcgcttttt ttagactttt caatacccct
    28201 ttttcccgtt cttttgtcga acgaaaattt ttctttccgc caatataagc gatcttcgta
    28261 tgtccgcatc caataccgta atctactgcc tgcataacac cggccgcatc atcggcagtg
    28321 acgttgataa ccgctggatc attgatgtga cgattgacaa caacgaaagg aaattctttt
    28381 tctttgagat agtcgatgaa aggctgatca tcatcacttt gactcataac gatcagtcca
    28441 tcgtagcgtt gcaggttgat ttctgttaaa tcaacaacat cgatactttc aacagataaa
    28501 ctgtaatttg tgtctaatac tgaatggatg cctgcaatgg aatctgctag gaaatttgat
    28561 gaagttcctt tatgcaaact tgagaaaaat agtccgatta gatatttttt ttgattcact
    28621 aaaccttttg cactatagtt tggcgaataa ttcatttcct tagctagttg tcgtattttt
    28681 tgttttgttt cattttttat taaactgctg ttattcaaag cacgagaaac tgtcgtatga
    28741 gaaacgccag ctgctttagc gatatctttg atcgttacca ttccatcatt ttccttactt
    28801 tttcactttt tgttaacgca ttcatcatat tacgataact acgataagtc aatagatctt
    28861 tgatatgaaa agattgcaca cgttaaaaat tacttggatt tttattttta attatgctat
    28921 tcttgaaagc ggggaatgct catttaatta gatgagtatc aattttacag aaaaatgaaa
    28981 gccttttatt ttaaaaggag gagaggaaga aaatgaagaa aagtacagta ttttataaaa
    29041 tatttttacc gatgttttta ttaggagccg tacttgtcat aggatttagt ttattcattt
    29101 accagaatac ctatgaatcg atcgaatcga gttatttaac agacaaaaag aatttgctca
    29161 ggcaagttaa aactaacgtt gaatggaaaa ttagaacgat tgaatattcg ttttctacgt
    29221 atggttcgac caaaaatttc tcggacatat tcaagcatcc gctgacatat acagattact
    29281 ccacttattc tgaaatcagg aaagaactaa attttataga aacgattgtt atggatgata
    29341 ataattatga tttagtcagt ttggctggta aatggggagt aatcaatgga tcgttgagtc
    29401 aactgaccga agaggaagtt gaaaagtatc aacagaagta cataaataac aaaaataatt
    29461 tattttggca aaagaaaaac aaaggaatcg aaatggtgat ttctttgcca atgtttgaaa
    29521 aagaaaaata tgcattggga attgctacga tagagaaaca tacaatagaa caagttgttg
    29581 gtgaaagtgg agaagacata ctttctattg aaaacgaagt tggcaacctt tttactactc
    29641 agacagtaaa aaataaagag caaccaactg tattaacgga cgtggatcta gattacaatc
    29701 agctgaaagt gataagagaa aaagataaaa cgtatatagt actgaaatca gattataata
    29761 attggattta cagattagaa gttgactcta gtgcaattgg cgcaatgatc cgcaatctga
    29821 gaatcgggct agttgcagtg tctttaacac tggtaggact cattggtctt ctttcctact
    29881 tattttcgga cagatttgcc cgtccgattt ctcaaataca ggaacgtctg aatcttaaat
    29941 caaaaggatt cagtgggaaa gaactagatt tggtcgctca atcagtcagt cggatcatag
    30001 gagaaaaaga agcgttaagt gcacacttag ttacccaaaa gccacagctg gaaacgctat
    30061 ttgtacttag tttatttaga aatcgagtag aaaggcgaga actagatcag cggttgcagc
    30121 aatttggtta tagttctcaa aatgactgct attatacagc tttggttcaa atcgatctgt
    30181 tagaagatag tcacggtggg gagcgtgatt tgctactttt agcgatcaac aacatgatca
    30241 cggaaattgt gccaaaggaa aatcgaatgc tgccgatcgt tttgaatgaa gaaatgcaag
    30301 ccactattta tcgaatggat aaagatgatg cagaagcaag caaaaaagtc atggagtatt
    30361 gccgactcat tcaaaaacgt gtaaaagaat acttgaattt aactatcagt ataggcatca
    30421 gtaaccggtt cgaaacgctg aatgaaagca agcaatcggt tgatcgggca aaagaagccc
    30481 tgtattatcg tgtaaatact ggtccaagtt caattatttt ttatcaggaa atcgttcctg
    30541 ctgctcatga aaaaacactt attagatatc cgatagaaga acaaaatcga ttgtttgaag
    30601 ccattcgttc tggaaatcag gaagtgaaga atttagtaca tgaattgatt gatgcattgt
    30661 ttgcacaaaa taaaaatcct ttgagtcggg aagtagtttc ggtccgcttg atcaatgaac
    30721 tcgttcaatt aggacagtta ttgggagtag attcaaaaaa ctttgataac atgaaacaaa
    30781 tttatgtaaa agcactgaac aattatcatc ccaaagaact agaaaaactt gttttagaac
    30841 aattagttct gccaatcaca gaatgctctc aagcaatcac tgatcatgaa tttaaaaatt
    30901 tgtcggagaa tattatgcat atcgtctaca cagaatatga tcgtgatctg tctttagatg
    30961 tgatcgctga taggttgcat tataacccta actatttaag taatgtattt aaaaaggaaa
    31021 caggagaaaa atttggtgat tttgttcaaa atcatcgttt agaggtagca aaaaaatggt
    31081 tgaaagagac taacctctcc gtaaaagaga ttgcagaaag attgcaatat cgaaatccgc
    31141 aaaattttat tcgtttcttt aaaaagaagg aatcgattac gccgggagaa tatcgaaaaa
    31201 aatatttgtg aatcaagagg ctgtttttct tgtggactga ttcaagcaat aaaagaagtt
    31261 agaataaccc tagtatttgt atttatggat agttaaattt caaggctaag cacgcaaaaa
    31321 gaactgtgac aaaagtcttg tcacagttct tttttacgaa taaatggcgt tcaagagaca
    31381 ggctatcgac aataaggcat aattttgtga aatatgggaa gctatttttc aaaatcgctt
    31441 cttattgctc ctgtgggtta ctcgtcgcaa tgtttgtgag agtttttatc atcgctcaag
    31501 gctgaacgtc tctttcacaa cctctgatac acggtgttcc aggagtagat tcttcgaaaa
    31561 ataagccgaa atttcacaaa aatttaagaa gcaattttcg taaatgcctt cttatttctt
    31621 gaagctgacc acttctgaca caactaccat tttgcgttca gtacataatc accctttgac
    31681 agaacctacg agtgctcctt tgacaaaata tttctgaaca aaaggataaa caatcagcat
    31741 aggcactgtt gcaataacga taacagccat tttaactgtt tgtgctggtg gaatcacatc
    31801 aacggcacta ctttctgctt gcatcccact tgatacaatg acgatttggc gtaaaagaat
    31861 ctgtatcggc catttagatg aatcgttgat atacaaaata gcagtcatat aagtgttcca
    31921 gtaagtaaca gcataaaaaa gcgagatagt agcaatagac ggtaacgcta atggcagcat
    31981 aattttgaaa aatacaccaa aatctgtgca accatccatt tttgctgatt cttccaaact
    32041 atccggcaag gcttggaaaa agtttctcat aatgatcata ttcattgcat taacagccac
    32101 aggcaggatc atggcccagt aagaattgac cagccctaga gaacgaacaa ctaagaacgt
    32161 tggaatcatc cccccgctga acaacataga aaatacaaca agaaagttga cgaaacctcg
    32221 accaaacaaa tactttctgg aaagaccgta ggccatcagt gaagttgcac acatactgac
    32281 gatcgttccg acacctgtaa caccaatcga aacagcaagt gctctaaaaa tggtcggcgt
    32341 agaaagaatg tagcgatacg cgtctaaaga aaaggtagta ggaaaaagga taaacttttt
    32401 cgcgacgact tcttgtgtag aagcaaaaga tgtagcgatg atatttaaaa aaggaacgat
    32461 acagatcaaa gctagcaaaa ttagaaacgt attgttgaaa agtgtaaaac ttctttctcc
    32521 tatagagact ttctttttca tatagatagg ctcctcctca aaataaatta aatgtttctg
    32581 tttctatatg ttcaatttat catcggtgaa agattacgta tataatcatt tcctatgcta
    32641 agtgtcagat agaaaaaaat agaaggtttg ctatgcgtag tttatttgtg aaaaaataca
    32701 ctttatcgtt ttgtatagca caaaaatcat tgctatgtaa gcgtttttac ttacggtaaa
    32761 aatgattata tacgtaattc agatagttat ctatcatgaa gtcataagca gcaaacattc
    32821 acaaaactaa ggaggggctt atggaaagtg taaagaaaca agaaccattt caactgacgc
    32881 tatcgaaaaa agaagaaaag ctcagaagac gaaaaaaaat aattgcagga atcaaaacga
    32941 ataaattttt atatgtgatg attctgccgg gagtccttta tttcttgatt tttaggtatc
    33001 ttcctatggg gggactcgtt attgcttttc aagattatca accattttta ggaataatgg
    33061 gaagtccttg ggtcggtttc aaacatttca ttcgtttgtt tacagaaccg acattcttta
    33121 cattattaag aaatacgttg atcttgttcg gattaaatat cttcgtgttt tttccgatgc
    33181 caatcatttt gtctttacta ttaaatgaag taacgaacaa acgattgaaa aacgggattc
    33241 aaacgatcat ttacatccct cacttcatgt cttgggtaat cattgtttcg attacttatg
    33301 tatttttgaa tgttgacgga ggggtattga atgaattcct tgcaacaatg ggatttgaaa
    33361 aaatcagttt tctaacatcc cctgaatgga cacgcacagt atatatcgga caagtgatct
    33421 ggaaagagtt gggctggtct acgatcatct acctttcagc gataactgca gtcgatacac
    33481 agttgtatga agctgcagaa atggatggag caggacgctt acgcaaaaca tggcatgtta
    33541 cattgccggc aattcgtcca gtaatcatta ccttgctgat tctaaaaatc ggtcagacat
    33601 tagacttagg gttcgagcat atgtacttgt tattgaattc attgaatcga agcgttgcag
    33661 aaattttcga cacctatatt tatacagctg gattgaagaa tggacagtta agtttcagta
    33721 cagcaatggg attgtttaaa ggggtcgtag gattgtttat ggtaatttta gctaataaga
    33781 ttgccaaaaa gctaggagaa gacggcgttt actaagatag aaagagaggg aaagatatgc
    33841 tgcagtcatt agataaaata aacggaaggc taaatcgtat tttacagttg gtttatttga
    33901 attttctgtg gggcggttta gtcatcgcag gtttgggagt ttttactttc ggtcctgcat
    33961 cgtatgcgat ggttgcagtg attagaaaat ggctgcgctc aaaagaaacg tttcctttga
    34021 cgaaaacgta cttcacatat tttaaagaaa actatcgtga aaccttgctg atgagctgga
    34081 tatatggcgc catcgggatt gttttacttg tggatctttc tttcatacac aattggtatc
    34141 ttaggatcgc tttgtatatt gctgcgttca tatatgtgct ctcagcactc tatattttcc
    34201 caatcatggc tcattatgat tggaaaggat attttttcaa aatgaaaatg gcatttttat
    34261 tcggattctc acatcttcat tactcattag tgttgatatt aagtatggta gggatttatc
    34321 tgatgatcat ccaaacaatg ccaggtgttt taacctttat cggtggcagt ctcttctttt
    34381 ccgcagtcac gtggacagca aattctatat tttgccattt ggaatggaag aactcatata
    34441 cagaaaaaac agaagaaaac aaaaatatta ttaaaacagt gaaggagaaa atttatgaaa
    34501 aaagcaacgc tcgtaaaatt aggtcttagt tttgcaggaa ttctagcact tgccgcatgc
    34561 ggcagcagtt caagtaacag ttcttcagag acagaagatg gaaaagctaa aatcacttgg
    34621 ttaaatattt tgcatcatgc ttcaccgcct acagacacga ttgtgaactt gatcgaagaa
    34681 aaaaccgata cggaactgac attcaactgg attcctgatg cttcaaaaga agaaagatta
    34741 acaactgcat tggcttctga tgaactagga gatatcgtta cacttcaaat tgcgatgttg
    34801 aaaaactctt ctgtccgcaa tgcgttaaaa tcaggcgtat tctgggatgt atcagaatat
    34861 ttaaaagact ataaaaattt aagtcaaatt tctgaagata gaatcgaagc tgcgagtatc
    34921 gacggtgcct tgtacggcgt accgatacaa aaagactacg ctcgtgctgg cttagtcatt
    34981 cgaaaagact ggttggataa tctaggacta gatgttccaa ctaccatgga tgagctttat
    35041 gaagtagcta aacaatttac agaaaacgat cctgatggaa atggtgtgga cgacacagtt
    35101 ggttttggcg atcgtgcgcc tagtgatatt cgttatacca gctttaaact tttcacttct
    35161 tattttggag caccaaatgg ctgggctgta gacgataatg gcaaattcac tccagaattt
    35221 gatacagaag agtatatgaa agctttgaat ttttcaagag atctatttga gaatggctat
    35281 ttggcacaag actttgcagt tacccaaaag actgatcagc aagaacaatt tgcacaagga
    35341 agaactggaa tctacactgg aatggtggat atcaagaatc tgaagacgat gggtcaaggt
    35401 attcaagatg atatggaatt agtgcctgta aacaagatct cagatggtga aggcggcggt
    35461 tatcatgttt ggtcagaagg aaatggtatt ggcggcttgc tagcatttcc caaatctcaa
    35521 gtaaaagacg aagcacaatt gaagaaatta cttcaatttg ttgatgatct tttagaagaa
    35581 gatgtgtata aattgatgac tggcggtatc gaaggaacgc attatgaatt gaatgatgat
    35641 ggttctatcg aatttatcga tatggatctt tggcaacaag aggtgcaacc gttatcaagc
    35701 tctcgtccta gtgaagtgac gttctctttc aaagatacag accctgaaaa agaattctct
    35761 aatcaattga tcaaagaaaa tgaagaattt gctgtaatgg atccttctgt gccattggat
    35821 tcaccgacga acaatgaagt aggatcagaa attgaaaaaa tcgtcattga tgctacggtg
    35881 caatacatca tgggacagct tgacgaagat ggattcaaaa aagctgtaga agactggaaa
    35941 gcacaaggag gcgatcgaat cacacaagag tacgaggaag catacaaagc agcggcacag
    36001 taatatagac gattgggaca aatacttctt ggtatttgtc ccaaatccta ttttaatttt
    36061 taaaggagag caaataatgg aaaactggtc aaagaaatta gcaaaaagta tcatggaaag
    36121 aacacctcgc ttatatgaag aaaagtggta taaaggaaaa tggtcttatg actatggcgt
    36181 cgtacttaaa gggttccaat tactttggga acgaacccaa gaaaaaattt attttgattt
    36241 cataaaagac aatatcgact atttcgtgca agaagatgga acgatccgtg gctacagcgt
    36301 tgaagaatac aatatcgacc atgtaaatac aggaaagctt ttctttttat tgtacaaaga
    36361 aacaggcgaa gaaaaatata aaaaagcagc agaactcctt tcccagcagt tggcaaacca
    36421 tccacgcaca tccgaaggag ctttttggca taaagaaatt tatccttatc aaatttggtt
    36481 ggatggctta tatatgggtt ctccttttta tgctgaattt atccagaatt ttagtgaaga
    36541 aaaagattac caagatgttt tgagacagtt tgaaatcagt tataaacatc tattcgatcc
    36601 aaaaacaaat ttgctgattc atgcatggga tgaaaagaag gtccaaccat gggctgatcc
    36661 aaaaacaggt ctatcccatc atttttggag ccgatcgatt ggctggtatt tgatggctgc
    36721 agcagatacc tatgaattac ttcatgaaga aacagattgt tctttgctga aagaaattct
    36781 agaaaagaca ctggctgcat tacttccata tcaagaagaa aacagcggga catggtatca
    36841 agtaacaaca gaaacaaaac ggaaaggaaa ttatctggaa gcttccggca gtagtatgtt
    36901 tgtttacgca atggctaaag gaatacgttt gggactgctg aacaaagaag aatggcttcc
    36961 acaagtgaag aaatcacatc aaggattact tgatgaattt gttctggaaa caaaagaagg
    37021 ctggctgaac ttgaataaaa actgtcaagt agcagggtta ggcggagcag atcagcgaga
    37081 tggttcatat gcttactaca tcagtgaacc aattatttgt aatgatcaaa aaggcgttgg
    37141 tgcattcttg caagcattga ttgaagtgga ggcattataa atatgtatga tattttgaca
    37201 tttggcgcat caattgatga attgaatact gaagcaatac aacaagccat tgatgctgca
    37261 gcaagtgacg gaggcgggac cgtagttgtt ccagcaggtg aatttctaac aggagcttta
    37321 tttttaaaaa gcaatgtcga acttcattta agcgcaggag cggttctgaa attctctgac
    37381 gatccaaaag attatcctgt tgttcattca cgttgggaag gagtccatcg aaaagtttat
    37441 gcttcctgta tctatgctca aaatgtagag aacatctctg tcactggttt cggtacatta
    37501 gatggaaatg gaaaaaaatg gtggcatact tttagaaatg aaccagataa tctcgcttat
    37561 cctcgtccta aattgatgag tttccataac tgtcatcgaa ttactgtgaa agatattaaa
    37621 ttgatccagt caccaagttg gacgatcaac ccgattcttt gcagtaatgc aacgtttgat
    37681 aacttgacga tcttgaaccc agcggattca ccaaatacag acggaattga tccagaatca
    37741 tgcaaaaatg tgcgtattag taattgccat attgacgtgg gagatgactg tattgcaatc
    37801 aaggcaggga ctgaagatac gtacgaaagg atcgcttgcg aaaatatcac gatcacaaat
    37861 tgcacgatgg tccatggaca tggcggtgtc gtattaggaa gtgaaatgag tggtagtatt
    37921 cgtaatatta ccatttcaaa ttgtatcttc caagaaactg atcgaggaat acgattgaaa
    37981 tctagacgag gtcgaggcgg gatcgttgaa gatattcgag tatccaatat agtcatggat
    38041 aatgtgatgt gtccatttat actgaatctt tattatttct gtggtccgcg agggaaagaa
    38101 ccgtatgtat gggaaaaaaa ggcttatccg atagatgaac ggacaccagc attcagaagg
    38161 atccattttt cgaatatcac ggcaaggaat gtccatgctt ctgcaggttt tatttatgga
    38221 ttagcggaac aattcataca agaaatcaca tttgacaata tcgatgtctc gatggcccaa
    38281 gatgcagttc ccggtgttcc tgctatgatg acaggaatag aggaaatgag cagacaagga
    38341 ttttatattg gctttgcggg taaggtttcc tttaaccaga tgtcgattga aaatcacgag
    38401 ggaccagcat ttctattaga aaatactaaa gaaatcagta ttgagaaatt ccaatcaaat
    38461 caatcagaaa atatggatga actggttgtt cgtaaaaact ttgcttatgc agttgaatga
    38521 ttttttaggt gctccatcta gcagacatac ccctagtgga aaacgattat accgagaaaa
    38581 acaaaacggt tttatgcttg aatactatca attgtactta aatgaattgg aaacagtacc
    38641 agccctctat gcttatccag aaaaagaagg accgtttccg ccggtccttt acctgcattc
    38701 tcacggcgga gatttttcag tcgggaaaag tgaattgatc catggtgctc cttacttagc
    38761 ttctccatct tttgcagaag tactgacagg aatgggatat gctgtttggt cgattgatgc
    38821 ttggggattt gaagaacgcg gaggtatttc tgaaagcgag ttgttcaaac aatttttatt
    38881 aacaggaaaa acgttatggg gaatgcgtat ttatgatgtg atcagcttga tcgattattt
    38941 ggagacaagg gaagacacag atactcaacg gctcgcaacg attggtcttt ctatgggagg
    39001 cttattaagc tggtgggtga cagcattgga ttctcgtgtc aaagtttgta ttgatctagc
    39061 ggcacaggtg gacatcgaaa tacttttgct acatcgccgc ctagatcatc atgggtttta
    39121 ttactatgtg ccaaatctat tgacagctta tacaacgtta gcaatccaag aaaaaatcgc
    39181 tccgcgccct cgtcttagct tagttggaaa aaacgatacg atgtgcttgg acgaaggcgt
    39241 actgaagctg agaaaagggc tggacaaaaa gtatcaatca atgggtagtc cagaaaactt
    39301 ttcttctttt tctttaacag gcggtcatat ggaaacacaa gaaatgagaa atatttggaa
    39361 acaattcata agggagcatt tatgaggatg aaagacaaac tgataattgg aaagaatgct
    39421 atagataccc cgacggattt tgaaactatc caagaaggaa tcgattattt ggcaggcctg
    39481 ccggatagcc atccaaaggt cttgatagta aaagaaggaa gatatgatga atatatagaa
    39541 agcagattat ccaattttaa acttgtggga gcaggaacag tagttatctc cggtaatcgt
    39601 tttgcaaaac aggagtatct tgacggtagc cagttaggga ctttccgaag tgctactttt
    39661 ttcttagaag gatcgaatgt cgtattagaa aatcttcaca tcgaaaatac agcaggtcca
    39721 ggagaaaagg taggacaggc tgttgcttta ttcaattcag gagatcgcgt atctttaaaa
    39781 aactgtcgac tatctggtta tcaggatacc ttatgcacgg gtccgttgcc tgatttgcaa
    39841 aaagatggta ctcctttttg tacaccggct tctgccaatc ctgagtattg tcgtcagacc
    39901 tatgaatatt gctggattga aggaacggtg gatttcatat ttggtggggc agatgccgtt
    39961 tttcatcagt gtcatatcca tagcttagcc agtaaaaatc ctggatacat cacagcagca
    40021 tctacgccga aaaatcaaaa aacagggtat cgttttgaaa gctgtacctt gactgcagaa
    40081 cctgatacta gaaacgtcta tttaggaaga ccgtggcgtc cgtatgcaaa tgtcttattt
    40141 gaagcgtgta agataggtgg gcatgttcac ccaactggtt gggataattg ggatgacaag
    40201 aaaaatgaac agactgtcaa attctctgaa cgtaataaca attatgaaac atcgatcaaa
    40261 agacctgaat ggatacattt agaaaagcag ggggttcaga aatgaaaaga aaatgggaac
    40321 tattgctagg aatggtcggt ggcagtctat ctttgatttt ctttggcggg ttagcagtta
    40381 ctttaagtaa tatgtctgcg tcagagttta aaaaaagtta tcagtcgctt gctgtagatc
    40441 attcaacttt gtctttagaa aatacatttg gattattaca ggacatgact ggtctgtttg
    40501 ccgttgtgtt gtttatttca cttgcttttt tagcagtagc attgtttttg actgctaaag
    40561 gaaaatactt aacaactgcc actggtttgt attttataac aggattcata ttgttgattg
    40621 gcactcagtt tattgcgttt ccttttgcat ttttttattt cgcagctggg gcattcagtt
    40681 tatatcgagt aagaatgaga aaaggagcct gaatcatgca gactatcaaa gtgaaaaatc
    40741 ctattttaag aggattcaat ccagatccta atatattaag agtgaaagag cggtattaca
    40801 ttattgtctc ctcttttgaa tggcttccag gaatccgagt atatgagtcg aaaaatttag
    40861 tcaactggtc acatacaacg gatattttga ccactcaagt atctttgcaa ggaaatccta
    40921 caaatggaag tatatgggcg ccacaaatca gttatgctga tggattgttt tatcttgttt
    40981 acacagatgt caaaagtgca ggccgtccgt ttaaagatgt ccacaattat ttgatcactg
    41041 catctgagat cacaggtcct tggtccgaac caatttattt aaatagtaac ggctttgatc
    41101 cttctttgtt ccatgatctt gaaacgggaa aaaaatggct tgtcaatgaa ctatgggatt
    41161 atcgtctgga cacaccgaat aaatccagcg gtatcgtatt acaagaatat gatgcagaga
    41221 agaagaagct tgatggtccc atctataaaa tatttgcagg tacagaatta gcgaaaacgg
    41281 aggcaccaca tttgtatttt cgtaacgggt attattattt gatgacggca gaaggcggta
    41341 caggaagagg acattcagtt acagtttgcc gagcaaagaa tattactggt ccttacgagc
    41401 ttgatcctgg ttttcctatg ctgacagcta gtgacaaacc tgcttctaca ttgcaatgta
    41461 caggacatgg gagcttagta caaaccccgt caggaagatg gtacatgaca tatctttgta
    41521 ccagaccatt aaacggtgcg gctattttgg gaagagaaac cgctattcaa gaagtgtatt
    41581 ggacgtccga cgattggtta agacttaaag atagccagac aactccgatg gaagaaataa
    41641 tgattgagac aaatgaagag gtacaacaag aaaaagatca tcagtttatg gatacatttg
    41701 aaggagagct aaagaagaac tggaatgcca gacgcatttt gccaaatgct gattggtgtg
    41761 atacaaaatg tagaccaggt tttttacgtc tgatttccgg tgaatctctt caatctacct
    41821 tcgaacatca tttgttagct attcggcaaa cggattttac ttttgatgcg gagacagcat
    41881 ttgaatacat gcctaataat tttaatcaga tggctggact ggtactgtac ttaaatgaat
    41941 ccaattatat ttattgttat gttacttgga atgaggacaa gggaaaatgc ctacgtatga
    42001 tagaatcaga aaacggagta gcacaaatag aagattggac gattccactc acagaaaaga
    42061 agactttttt gaaagtcact gtaaagaaag agacagcaca gtttcatttg aaagacgtat
    42121 caactgaaga atggaagaaa gtaggtccgc aaaaaaacat gtgtatactc tcaggagggt
    42181 ttactggaaa ttttatcggg atagcagtgc atgatctaaa taaaaaaaga ggaagttatg
    42241 ctgattttga gtttttcaag tatgatggaa aagatcatct gtaatttaat tctttaataa
    42301 atgaggttca ctaacttatt ttcactagtt agtgaatttt tttgtttgta tatattgtta
    42361 tgtaaaaaaa gttatcaatc ttatgcatca atgtttttag attatttttt gttttttatc
    42421 tgtcaagata atgcaataaa aagccaacat tgtgaatata aaaacaagaa aaaaagagtt
    42481 ataattttga aaacgtttac tagaagaggt aggaggaatt caaatgcttt cacaaaatgc
    42541 tgttacagca aatacaataa aaaaagaaaa aaaggaattt gggatttccg ataagatagg
    42601 ctacatgttc ggtgatctag gtaactgttt tattttaggt ttagtcaata gtttcttgat
    42661 gatttattat acgaatgttc ttggcgtttc aggggcaatc gtaggtacgt tattcttgat
    42721 ttcgagaatt ttggatgcgt ttgctgatgt gatggttgga agattatgtg atgtctcaga
    42781 gttaacagaa actggccgat ttattccttg gatcagaaaa atgaagtatc cattttgctt
    42841 agtgactgtc attttgtttt tgccattcgt ccaacatctt ccaatgacgg tgaagattgc
    42901 ttatattttt gtttcataca ttgtttatgg gatttttctc tcaacaatca atatcccgta
    42961 tggagcaatg gcagcagcaa ttagctcaaa tcctaatgac cgcacctcgt tgtccacgtt
    43021 ccgaagcatt ggttctgcga ttggcggatc aactacagga ttcttcattc cgattttaat
    43081 gtatacccat aacaagagtg gacaacaagt cgtttcaggg aatcatttct tttggatttc
    43141 aatcggatgt gctgcaatag catttgtcgc atatatgtta acttgtaaat tgactactga
    43201 aagagtaaga gttgtcaaaa acgaaaaagt atctgcttct cagttggtaa aaggtttgtt
    43261 agctaatcga gcactgatta ttttagtcat tgttgacatt ttcattgtga tcaatcagat
    43321 tttagctggg acgaatatga cttatttatt caatgattac ttccataata aacaagcaat
    43381 gtctgttgct cttttattta cttacggaac agtagtagtt ttagcaccat ttgctacaaa
    43441 gctgacaaaa ttatttggta aaaaggaagc aagcgtattt gcgttacttg cttcttcatg
    43501 tatgtacttg ctgatgtatt tccttcatat aacaaatgca tgggtatatc tgattctttt
    43561 atttatcgct actttaggat ctggtttatt caatttgatg gtctgggcat tcattactga
    43621 cgtgattgat tatcatcaat ataagacagg cttaagagaa gatggaacag tatatggagt
    43681 aaattctttt gcgagaaaag taggacaagc atgtgcagga gcggtaggcg gatttatgct
    43741 ggcactcatt ggttaccaat cgtctacaac aggtggagcc atccaatcag ttttagtaca
    43801 agaaaggatt tatacgatgg caaacttgtt accagcattc tgcttatttg tatccgcaat
    43861 cattttgcta gtaggttacc cactgaatcg ttcaaaaact gtatttattg gtgaagaatt
    43921 gaaaaaaatc aatgaaagat agggagaaac gtattgcgga caaaaaaagg gattatcaat
    43981 gctttgaact ttaaaacaaa cgacactcaa caagatgata caataatact gcaaaaagca
    44041 atcgatcaag gtgcgaaaga acacttacct gtatttattc caaaaggcat ttatttagtc
    44101 ggagcccttt ttttaaaaga taagagtcat cttatctttg aagaaggtgc agtcctcaag
    44161 gggagaacag atatcgaagc atttccagaa atcgatacac gagtagccgg cgtagaaatg
    44221 aaatggccag cagcgatttt gaatgttctc tcggcgaaag atattcttat agaaggaaaa
    44281 ggaataatcg acggtcaagg agaccattgg tgggagctgt attggggaaa agatcaaaaa
    44341 agcgggacaa gagctgaata cgatcaaaag ggacttcgtt ggattgcgga ttatgctatt
    44401 aaacgtccac gcgcttgttt actgtatcat gcagaacatg ttgttgttcg tgatttgatt
    44461 tttcagaaat caggtttttg gaacttgcaa atcacttatt ctaacgatgt attagtagaa
    44521 aaagtaatca tccgcaacaa tgatggacca agtacagatg ggattgatat tgattcttca
    44581 acaaatgtgc gtgtctatga atgtgatttg gcctgtggtg atgattgtat tgctattaag
    44641 tctggcagag atggcaatgg tgcaagagtc aatcgaaaat catcaagaat cgaggttgct
    44701 agatgtaaaa tccgttcagg atacggtgta actattggta gcgaagttag tgctggagtt
    44761 agtgacgtat atattcacga tatcgatttt tttcagtcag actgtggtat tcgaatgaaa
    44821 tcttcaaaag aaagaggggg agtgatcgaa aatatcaggg tagaaaatct gaatatgatt
    44881 gatgtacaat ttcctttttc ttggataatg gactggcata atgaatacaa tagaaaaaat
    44941 agcaatgacc ttgctcaaat gccagaaagt tggcaagcag tggctgaaga aatacctgaa
    45001 tcaaagcaaa tgacgaaagt caaagacata cacatcaaaa acgtacaggc atgcctttct
    45061 ccaggttact cacttccttc ccgagctttt gatttagtag catttcctga aaagcctata
    45121 gaagatgtct gcttttctca ttgccaaata actgcaaaag aattcgggcg aatcgaagca
    45181 gtacaaaact tatgtttcaa aagctgtgat ttatctatag aaactggcaa cacagctgca
    45241 aacaatactt ttgacaatcg ttgaaattat aataaggtca agcttaaata atattttagc
    45301 ttgaccttta ttttttttaa tgatatatta aacaacgttg tcgcgcttca ttgaaacatt
    45361 ttatgaaaat tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc
    45421 gt
//
LOCUS       DAYYNQ010000009        44137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000009 DAYYNQ010000000
VERSION     DAYYNQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.009"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1879)
                     /locus_tag="ACHBJ1_000488"
     CDS             complement(<1..1879)
                     /locus_tag="ACHBJ1_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9429461.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VE"
     gene            complement(2042..4120)
                     /locus_tag="ACHBJ1_000489"
     CDS             complement(2042..4120)
                     /locus_tag="ACHBJ1_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9429462.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(4133..4408)
                     /locus_tag="ACHBJ1_000490"
     CDS             complement(4133..4408)
                     /locus_tag="ACHBJ1_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429463.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(4770..5225)
                     /locus_tag="ACHBJ1_000491"
     CDS             complement(4770..5225)
                     /locus_tag="ACHBJ1_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429464.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(5379..6587)
                     /locus_tag="ACHBJ1_000492"
     CDS             complement(5379..6587)
                     /locus_tag="ACHBJ1_000492"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM9429465.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(6699..7031)
                     /locus_tag="ACHBJ1_000493"
     CDS             complement(6699..7031)
                     /locus_tag="ACHBJ1_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9429466.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            7179..8054
                     /locus_tag="ACHBJ1_000494"
     CDS             7179..8054
                     /locus_tag="ACHBJ1_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9429467.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(8136..8717)
                     /locus_tag="ACHBJ1_000495"
     CDS             complement(8136..8717)
                     /locus_tag="ACHBJ1_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM9429468.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(8723..9982)
                     /locus_tag="ACHBJ1_000496"
     CDS             complement(8723..9982)
                     /locus_tag="ACHBJ1_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9429469.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(9984..11030)
                     /locus_tag="ACHBJ1_000497"
     CDS             complement(9984..11030)
                     /locus_tag="ACHBJ1_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429470.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(11265..11540)
                     /locus_tag="ACHBJ1_000498"
     CDS             complement(11265..11540)
                     /locus_tag="ACHBJ1_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM9429471.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACHBJ1_000499"
     CDS             complement(11783..13093)
                     /gene="der"
                     /locus_tag="ACHBJ1_000499"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM9429472.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACHBJ1_000500"
     CDS             complement(13275..14504)
                     /gene="rpsA"
                     /locus_tag="ACHBJ1_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM9429473.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACHBJ1_000501"
     CDS             complement(14639..15319)
                     /gene="cmk"
                     /locus_tag="ACHBJ1_000501"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM9429474.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(15368..16003)
                     /locus_tag="ACHBJ1_000502"
     CDS             complement(15368..16003)
                     /locus_tag="ACHBJ1_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM9429475.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(16069..17472)
                     /locus_tag="ACHBJ1_000503"
     CDS             complement(16069..17472)
                     /locus_tag="ACHBJ1_000503"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM9429476.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(17459..18490)
                     /locus_tag="ACHBJ1_000504"
     CDS             complement(17459..18490)
                     /locus_tag="ACHBJ1_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429477.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            18521..18742
                     /locus_tag="ACHBJ1_000505"
     CDS             18521..18742
                     /locus_tag="ACHBJ1_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM9429478.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(18907..19644)
                     /locus_tag="ACHBJ1_000506"
     CDS             complement(18907..19644)
                     /locus_tag="ACHBJ1_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM9429479.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(19797..21137)
                     /locus_tag="ACHBJ1_000507"
     CDS             complement(19797..21137)
                     /locus_tag="ACHBJ1_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9429480.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(21374..22897)
                     /locus_tag="ACHBJ1_000508"
     CDS             complement(21374..22897)
                     /locus_tag="ACHBJ1_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM9429481.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(23275..23886)
                     /locus_tag="ACHBJ1_000509"
     CDS             complement(23275..23886)
                     /locus_tag="ACHBJ1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9429482.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(24009..24134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(24216..24932)
                     /locus_tag="ACHBJ1_000510"
     CDS             complement(24216..24932)
                     /locus_tag="ACHBJ1_000510"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9429483.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACHBJ1_000511"
     CDS             complement(24938..25507)
                     /gene="scpB"
                     /locus_tag="ACHBJ1_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM9429484.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(25491..26261)
                     /locus_tag="ACHBJ1_000512"
     CDS             complement(25491..26261)
                     /locus_tag="ACHBJ1_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM9429485.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(26258..26629)
                     /locus_tag="ACHBJ1_000513"
     CDS             complement(26258..26629)
                     /locus_tag="ACHBJ1_000513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429486.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACHBJ1_000514"
     CDS             complement(26648..27535)
                     /gene="xerD"
                     /locus_tag="ACHBJ1_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM9429487.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(27631..28104)
                     /locus_tag="ACHBJ1_000515"
     CDS             complement(27631..28104)
                     /locus_tag="ACHBJ1_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9429488.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(28209..29072)
                     /locus_tag="ACHBJ1_000516"
     CDS             complement(28209..29072)
                     /locus_tag="ACHBJ1_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM9429489.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(29268..31367)
                     /locus_tag="ACHBJ1_000517"
     CDS             complement(29268..31367)
                     /locus_tag="ACHBJ1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM9429490.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACHBJ1_000518"
     CDS             complement(31587..32696)
                     /gene="rpoD"
                     /locus_tag="ACHBJ1_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM9429491.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACHBJ1_000519"
     CDS             complement(32720..34561)
                     /gene="dnaG"
                     /locus_tag="ACHBJ1_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM9429492.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(34743..37058)
                     /locus_tag="ACHBJ1_000520"
     CDS             complement(34743..37058)
                     /locus_tag="ACHBJ1_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9429493.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(37243..38103)
                     /locus_tag="ACHBJ1_000521"
     CDS             complement(37243..38103)
                     /locus_tag="ACHBJ1_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM9429494.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(38162..38794)
                     /locus_tag="ACHBJ1_000522"
                     /pseudo
     CDS             complement(38162..38794)
                     /locus_tag="ACHBJ1_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            38961..39722
                     /locus_tag="ACHBJ1_000523"
     CDS             38961..39722
                     /locus_tag="ACHBJ1_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9429495.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            39748..42339
                     /gene="mprF"
                     /locus_tag="ACHBJ1_000524"
     CDS             39748..42339
                     /gene="mprF"
                     /locus_tag="ACHBJ1_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9429496.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACHBJ1_000525"
     CDS             complement(42527..43714)
                     /gene="tuf"
                     /locus_tag="ACHBJ1_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9429497.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT        12289 a         9358 c         7523 g        14967 t
ORIGIN      
        1 cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt tgtgcttctg
       61 tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct tgatgattca
      121 tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct tcaatcgttt
      181 cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc ttgctctgta
      241 tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt aaataactgg
      301 acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat aacttatctg
      361 ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct ggaaaacttc
      421 taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc ggttcatcca
      481 ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg cgtttttctc
      541 cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa aatcgttcaa
      601 gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca gcagcttctt
      661 gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt gtataatacg
      721 caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt ctgccagcta
      781 gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct agtctttccc
      841 gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata cggtaactgc
      901 catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc agctctaaat
      961 catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg ttgatccgtg
     1021 cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat tcctgtttat
     1081 aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct gctttttcta
     1141 agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa agttcgaaga
     1201 ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat agtgcgccac
     1261 ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa tggtttgttg
     1321 gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga ctaactcgtt
     1381 ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt cctaattttt
     1441 gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc atttgttctt
     1501 ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct agttcgatca
     1561 atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga aagactgctt
     1621 ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca atacggtaat
     1681 cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg gcaataatat
     1741 ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg tctttttcgt
     1801 ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt ttgaggtcag
     1861 ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc ataaaaatag
     1921 tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa aaagaacagg
     1981 accgattcga tcctgttctg atatgttttg ttttttataa aacttgaaaa tttttacctt
     2041 tttattttat tggactcgtt ttttttgact ttgctcgaaa cgcaaatcgg aaagaaaagt
     2101 tatcagatgg tttgttcatc gtataagatt ctaacaaatt gatattcctg cgatagctgt
     2161 tcgcagaaaa cgtcgtgatt ttttcagcaa cttcgtattc atcaatgatt tttctttcaa
     2221 gataataacc tcttagcatt tttttcgtaa acagactatc ttgctggatc atccacgaag
     2281 aaatcaaatt ccccgagttc atttttattc cttcaagttt gcgttgctgg ataaaaaatt
     2341 gaaccatcgt ttcatcataa atatcagtca agttatctaa actttcaata atcaactgag
     2401 tatttttttg atatgtctct ctgatttctt ggatatcttc atcactaata aatgtctgac
     2461 gatcagcata tcttctttgc caaaacattt tcggatggag aacgattcgc attaacctac
     2521 ggacaacaat gaaccaaaag ccgataaaag acaaaatctg gcttgtgata gaatgttcga
     2581 atcgagaaag aaaacgggag taaaaacgat aaccattaga agaaagcttg cccgaacgat
     2641 aactttcatc caatccatcc cgttcgatcg acaaaatcag tgcttgtatt tcttgtactt
     2701 cttgcttttc atcatcggtt aaatcttcca agtacagatc acgtatccgt tcttgataat
     2761 tttctatcac tgcttcagtg gctaaaaatt cttttgtatt acgatcaatt tcctttattt
     2821 cttttcgcaa aacttcaacg acctcttcta aaatcatcaa agcattcaaa tccgtgcttt
     2881 tcgcttcctc tgtttccgtc agcataggca agacaatgat ccctatgacc aaagtcacta
     2941 agatgacacc cgccgtcaga aacaaaagca aagaccgctg gtaaaacatc atattattga
     3001 ttgaaaatgg caaaataaaa atcgtagcca aactgactgt tccctttaca ccgccaaatg
     3061 ttaagatcaa gatctcgttc aactgttttt tgaacttttt actgccatct ttgaatacat
     3121 aaaaaagact gatagaaatg aatctgatca caaaaagcat gacagagatt aatacgataa
     3181 tcgctaataa aagaccgttt ggatataaac cgtctcccca tactggcgag aacacttgcg
     3241 tcaattcgat tcctaaaaat ataaatacta atgcattcag tgtaaaagca atagttgtcc
     3301 atgtactgtg ggacaagctc gacaattctg catcaaacac agagattttc ctaaaaccag
     3361 aagcttggag aatacctgct gccactgcag caatgattcc agatacatca aatgcctctg
     3421 ccaatacata ggctagaaaa ggaagcaaaa gttcgatcag cagataccct gtgacatctt
     3481 gtgctgatgc tttttcgatc aaatgaatta ttttttgttt gaaccagaca agcaaaaagc
     3541 cgatcaaagc tcccccaata ctagaaacta ctagactcat ccccgcgttt actgctgaaa
     3601 aagatccggt gatcagcgca ccaagagcaa actggaaggc agtaacacca gatgcatcat
     3661 tcaaaagacc ttctccttcc aaaatatgca tcgccttttt agggatattc actcgtccag
     3721 aaagagaact gactgctacc gcatctgtcg gacctaacgc tgctccaaaa gcaaagcagg
     3781 cagcaagtgg gacacttggc aacaggaatg ataacgtcgc acctaccccg acaagggtca
     3841 aaatgactcc tccaaatgcc aagaacaaaa ttgtcccgaa atttttgaga atcgaagaaa
     3901 tatctgcttt ttctccttct ctaaataaca atggtgcaat aatcataact aagaacaatt
     3961 caggttcaaa atcgattgat tgtccccact ctgtcagacc taataccact ccaaggaaaa
     4021 tttggatcag tggagctgga atcattggca aaatgcggct agctatgttc gataaggtga
     4081 tcgttactgc aaatacaata actaactcag caaattccat cgtccatccc ctttattttt
     4141 tattttgtgt aagcattttt aaaaattgtt cttttttctt taaaattttg gtttcgacta
     4201 cttcaacctg tgtttggttc ccctctaatt cacactgtac acgttttgac tccaaacgaa
     4261 gcagatcatg tttcagacgt tccaactgac gatacatcaa tatatttctt ttacgcaact
     4321 tacaaagtat ttgacggcgg tcatccatcg aaagggcgcc atagcgatca tgcaacagtt
     4381 ttttttgttg ttcaaaactc tctctcattt tccagatctc ctatacacaa acttccatca
     4441 ttatagcaaa gttctttttg gatgaaaaat atttcattgt ttcatcgttt attgctagtt
     4501 agaaatttcc ataaattttt caacgcctca ttctattcca aatagtatca gatatcaagt
     4561 aaaacttaag aaagataaaa gtttctgaca aaaggatgtt tactttccca ttcctgcctt
     4621 tcttgttctt ttatcacaac aaaaaaatgg ggctgcgaca aaaatcttgt cacagtccct
     4681 tattctgaat aaacggtgtg gaagagacag cctatcagta gtaataagac acatctcttt
     4741 cacagcctca tttttgcgta aaaatcgatt tattcgaaaa attccccgta tacatcttct
     4801 acacgttgat ctggattgat cacgatatac aagttacctg ttttttcgct gatttttgtt
     4861 gattgatatt gattgtctaa tccttctgtt cctttatcta gatatgggaa gtacacaaga
     4921 tcaggtgtgc catttcggta aaggatttcc attcgctcga tatcttcgta tttcaatgcg
     4981 cgattaaaca tgcccatttc cagtccgcct aggttgatat catgtgtcgc aactgcgtcc
     5041 ccttcatgga agatttcgat tttaaatcca gcacacggat ggatttcaac aaattcactg
     5101 ccattgattc gtccaaaact tgttgtgatt tgtttgatcc aaagatcgcc gatatgacgg
     5161 cggttgatcg tccaggtttc tccatttcta aaataaaatt ttagcgctgt catttcttta
     5221 ctcattaaaa agactccttt atgatgtaat ttattcttgt actacctcag tttagcacaa
     5281 atttatgtaa gtggtcacat cttatgtttt ttcttctaat gacgtatctg cccactttaa
     5341 gtagccaaca aagctgagca gatacagtct agtgtttttt aaatcaattt ttctttcgat
     5401 aaaaaatcca aaacctgttt tttatcattt tttattcttt gttctacgac ggctttttca
     5461 gctaattgca aagcttcact aacccactta ccaggttttt ttccagtttc ttctagcaag
     5521 attttaccat ctactgctaa ttcgcttaat gacttgatag gtaaacgatc ataggattct
     5581 cctacggcat ccatctgagg tgtttcgtca aaatagatca tcaattcttc tacttgcagt
     5641 gcatgttttt tgcctaagaa gtacaattcg aatggttcca aagctccagt ttctcgttgt
     5701 cgtagtcctt ggacaagtgc ttgaacgtct ttaatcattt ggttagaaca cttccacgat
     5761 ttcaaaaagg aacgaatctc attttctttt agatccagtt ggctgatcaa taatgcccat
     5821 gcttggctct cttctttcaa tgggacggat ggtaattcag caaaacgtaa aagtgcttcg
     5881 ccttcttctt tcaaaccggg acagcaaatg tagcactctg tttcaataaa tgcatggatt
     5941 cccgcggccc gttcatttcc caagagcatc ttgatgaatt ccacatttat acgttctaca
     6001 gatattttac ctaatagctc gtggttttct ttgatggcgt agaaggtttc ttcctctaat
     6061 ttgaacccta gttgactgac aaaacgcaac ccgcgcatca ttcgtaaggc atcttcatgg
     6121 aaccgctcat gaggattgcc tacagctcgc aagatctgtt cttctagatc ttgcagacca
     6181 tcgaacaaat cgatgatttc tccatcttct ttcaaagcaa acgcattgat cgtaaaatct
     6241 cttcttttca aatcttctgc caacgaacga acgaattcga catgatctgg tctacggtag
     6301 tcctgataag ttgattccgt ccgaaatgtg gtgatctcat attgttcatc attttctaat
     6361 accaacacag ttccatgttc gatcccaata tctatcgttt ttggaaaaag ctgtttgatt
     6421 tcttctggaa atgcgcttgt tgcgatatcc acatcatgga ttggctggtt cagcaagata
     6481 tcacgaacac ttccacctac aaaataagct tcatatcctg cattctctaa ttttttcaga
     6541 actggaattg ccttttggta ttccagaggt aattgtttta atttcatttt tccaaccttt
     6601 cctgcgtgtt tcttgtcctg agataaagac tcagttgcat ggtgactgtt tgacaagtcc
     6661 gtttcagagt cttcaaggcc aggaccactt catcttgttt actcgtcctt acgctcaaag
     6721 cgattatggt ctctttgatt gaatttttcc atgttttttt caaaaacttc agttaagtcg
     6781 atattcaatg aattagccat gatcatcgta acgaataaaa catcaccaag ttcttctgct
     6841 accgagttaa ctggttcaga agcctttttg cttttttctc cataagtgtg gttgacttct
     6901 cttgccaatt cacctacttc ttcagttaat cgagccatct gagctaatgg agagaaatac
     6961 cccaccttga attgctggat atattcatcc acttcctgct gcatactgta taaagaacgc
     7021 tttttatcca tctttttccc tccaaaacta tcttaccaaa actttaacgc aaatcctaga
     7081 taagagcaat tgagaaaatc gaatttccat gctacattta ggaaggaatt tctaaatgga
     7141 aattctgtga aaatacataa ttgaaagctg gaattcatat ggtcaaacga aaaacagtaa
     7201 aagaagttct ttttatcatt ataggaacaa gtatttatgc atttggtctt gtttacttga
     7261 acatcgctaa tcatttagca gaagggggag tctcagggat caccttgatc ttaagagctt
     7321 tatttggtat tgatccagct tatacgactt tattaatcaa catcccattg attttactag
     7381 gaggaaagat cctcggtaaa cgttccttgg cctatactgt tctaggtaca ctttctttgt
     7441 cattcttttt atggatctgg caaagggttc cactagaaat caatttacag catgatctac
     7501 tgatcgtttc tctacttgcg ggtttagttg caggtgtagg aagcgggatc gtctaccgca
     7561 tgggtggaac aacaggcggc agtgatatta tcgctcggat tttagaacaa aattacggca
     7621 tcactatggg gcgttcccta ttggcatttg atatcatcgt cttacttgct tcattaacct
     7681 atatcgactt gaaacgtatg atgtatacgt tgattgtttc gtatgttttc agtcgtgtga
     7741 ttgattcgat cttagatggc ggatattctg caaaaggtct acttgtcgtc tccaacaaaa
     7801 gcgaagagat tgcaccacta ctgatgaccg gtctgcaaag aggtgtgact ttcctttctg
     7861 gagaaggcgc attctctggt aattcaaaaa aaatcatcta tatggtcgtt agctctagtg
     7921 aattaactga agctaagagg atcgtccacg agattgacga aaaagctttc ctttcaatca
     7981 tcaatgtgca tgaagtggaa gggcaaggct ttacttattt gaagccacag acaagattac
     8041 tgaaaaaact ataagaaaaa agacaaagat aggctatgag gaaagattcc acatccagct
     8101 gacctttgtc ttttttagta ctatactatt tttaactaat tgttatgttc acttacccgg
     8161 ttacttttct tttgtgtctg caaaaggatc tttcggttaa cttcatcaga taatttcaaa
     8221 aaagcatccc tatctccatc ttctagtgct tgatcgattt gagcataaag catttttagt
     8281 tttgcctctt cttctttttc ttgaaaatat cgttcaatta attcagtatc ttcttcactg
     8341 actgaagaat cccaagaaga aaatggattg tcttctaaga tagatagata ttctcttgtc
     8401 tgccaagcat tttcgaatat acattccaca tataaaggct ttttccaatt caaacggatt
     8461 tcatgaaaaa tctggtctgt gtccgtaaat tcttttccct gcaaaaacaa tttcatgggt
     8521 tccccttcaa cagatagatc actgatctgc agtcctcgat ccgcaaaatt cgccccttca
     8581 acaaagtgga cattgcttaa aatcgcttca tgattggcaa gataattcaa gatccaaaaa
     8641 acttctcttc ggctaaaaga tacatgattg accatccacg ttaaaaaatt cttcttttcc
     8701 cgtacatcaa ttagcatttt tctcacctct ctgttaggtc atctaataat gacagtgcct
     8761 ccatatctcc tggttcaagt gttaaataat aagtcaataa ttcttgcgct cttttcagct
     8821 gcccttcttc tcttaagaaa aaggcatact ctttcaagaa atctggttca tgtgatagtt
     8881 catgataagc ctgttcatag tgtacagctg caacggtaaa gtcttctaat tcattataag
     8941 catgggctag gttccattct gcataaggat tagcagtttc ttccatctga ttgatcactt
     9001 gtatcacatc ttcatatctt tcatcatcca agtaaagatt acttaaagta agtaatgttt
     9061 catcttgttt gtctcctact tctaaagctt tcagtaaaag ttcttctgct cgctgtctat
     9121 catgcagacg gaaagcattt tccgaggcca ggtgataaag ttcgacttgg taaggatttt
     9181 ctttgattcc ttcttctaac actgtttgtg cttcttcaat catttcttct tcttgaagag
     9241 cttgtcctag atatagatat aacgattgat aatgcggatt aacttctcga agttcttcca
     9301 gatagttgat tgcctgctga ttttctttca attgaagatt cgtaaaagct agctggaaca
     9361 gtcgatcatc tgtcctttct tctttcagtg cttcttctaa aggtacgata gctgcttcga
     9421 attctccttg catacttaag gattgtccta aacgttcctg catcgagatt cctgaaattt
     9481 catttatccc gcttgccaac aatgtttcat aaaccactgc tgcctctttg aaacgatcaa
     9541 ctgaaaaata caattcagct agcgcaaact ggatcaatgg ttcatcagga agcaacccag
     9601 ctgcttcttt caattttgct tcactcactt cagggattcc gataacttgg tacaaatcag
     9661 ctatcgccaa taagctttgc ggatagtaat cactttgctt atccactttt tccaggtatt
     9721 caaaagctaa gtcgatctca ttatcttcga ttgcaatttc tgctaacggg atgttcagtc
     9781 cgtcattttc tgggtattgg ttgatcagtt gttcaaaaat ctgttttgct tcgatcaaaa
     9841 agccaatagc cagcaattct tcgcctagtt cagctaatat atctgctggg tcttctttta
     9901 atgctttttc taaaaagatt tgtgcttgtg acaagtcttc atctgataaa gcttgcaaca
     9961 ttttttcgct atttgtattc atttcaattc cctcttatct cactgacgat ttgttgaaat
    10021 accgtcattg ttctcaataa ttctttttcg tttggatgtt ggtatccgtc tattatacct
    10081 attttttcta aaacaagtat ttttttgctt ttttctgcca aaaaaagaac tttttccaaa
    10141 tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa gtccagccac
    10201 acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca cacgaaatga
    10261 aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt ttcggtcaga
    10321 taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc taattgataa
    10381 aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg ctttttattt
    10441 ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc gcaacagata
    10501 aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt ttgatcataa
    10561 acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag ctgcaaaatc
    10621 gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat catccatagt
    10681 gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat cccttcattt
    10741 ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa atcaagcagg
    10801 ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca gtcattgttt
    10861 gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata gcgctgattc
    10921 gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac tagcgtttct
    10981 ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat acgcatccct
    11041 cctcttctta tttttatttt accattagta agcaaattct agaaaagata agagcaaagc
    11101 tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt tctatgtatg
    11161 acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga aataaaaaga
    11221 aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt aacagcgtct
    11281 tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc gatttcttca
    11341 ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa gttaccaaaa
    11401 ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga aaatacagca
    11461 tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac tttttcgatt
    11521 aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat gatagaaata
    11581 tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt gattaacttc
    11641 aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt accaattttc
    11701 acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct tttgaaaaaa
    11761 aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg atggacgtcc
    11821 cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa gaaaaatgca
    11881 tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg acttgtgtag
    11941 cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt gctacagcat
    12001 ccataatgat atcattcaac acagccgaag agatacgaag attctggttc atacttactt
    12061 gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa acaaaaacga
    12121 ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa tcacgcattg
    12181 tattggtttc tttctttaca agatcccatt tattcacgac gataataatt ccacgaccag
    12241 cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct tcagcattca
    12301 gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc acgctgtatt
    12361 tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct atcattaaga
    12421 atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc gttccttcaa
    12481 tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag gatttaccaa
    12541 catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct tcagtctctt
    12601 cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca tggcttcctg
    12661 aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg tttctcattt
    12721 ctggattatc tactttattg actgctaaaa tcactggttt attacttctg tataaaattt
    12781 tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca caaatgatta
    12841 catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata aaaggttcat
    12901 cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac cattcacctt
    12961 ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag atacgctcac
    13021 cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg attgcaattg
    13081 ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt ttaacgaatg
    13141 cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt ccttattgaa
    13201 atactaatcg cttttcaaaa gaaaaactga gacaaaagca gctgctttca tctcagtcac
    13261 agatacaaaa tcaattattt ttcagaatca tcggttgctt tttcttctac ttcttctttc
    13321 aacgcatcac ctaaaatgtc tcccatagta aatccagtat tttcttctgg taattcataa
    13381 gactcaacat tttttggttc ttcttctgaa gctggttttt cttctaaagc tttgatactc
    13441 aatgcaatac gatgttcttc tggatggatc tctaatactt tcactttcac ttcgtcacct
    13501 tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga gatatggact
    13561 agcccttcta ctccagggaa cacttcaaca aaggcaccga agcttgtcaa acgtttaact
    13621 gttccgtcta atactgtgcc aactggagct tttgtatcga tttctgacca aggtccaggc
    13681 tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag gattgataga caacactttg
    13741 acatttacat catcattgac agccaataca tcacttggtt ttgctacatg actatgtgaa
    13801 atctctgaga catggactag accgtcaatt ccgcctaggt cgacaaatgc gccaaaatca
    13861 gtaagacgtg caacacgtcc actgagaaca tctccctcat ggatagttga aagaatttct
    13921 tttttctttt cttctctttc gttttccact accgctttat gcgataagat caaacgattt
    13981 tctgatggtt cgatttcgat gattttaaag ctcaatgttt tgcctttata gtctgaaaag
    14041 tctgcaacaa aatgatcttc caccattgat gcagggacaa aaccacgtac accgacgtca
    14101 acgactaaac cgcctttgac aacatttgta acaggtgctt cgatgacctt gccctcttgg
    14161 aaatctcttt cgatatcttc ccaaactttt ttagcatcta aacggcgttt tgaaagtaga
    14221 tagctgccgt tttctttatc tttgccgatc gtactgataa cgactaaatc taatacatca
    14281 ccgactttta ctacttcgct gatatcttct accggcaaag ttgataattc ttttgccgga
    14341 acgactcctt caactccagc cccttcgatt ccgacaatca cttgtttgtc ttcaatgact
    14401 aaaacttcgc ccttaacaac atcgccaact tttacttctt gtacgctttt cattgcgtct
    14461 tccattgact ggttgttctc ttgattttga tcaaattctg tcataacact tgtcctccta
    14521 accaacattc tgccgtaagt acggtactgt ttttagttta attgattatt caaaaaaaat
    14581 aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt ttcttaattt
    14641 aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg acttctggaa
    14701 tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc ggagagacgt
    14761 cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt tcaagatcag
    14821 tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca cttacgcttg
    14881 ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg tctcttccgt
    14941 ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat tcttctctga
    15001 cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga cggatttcta
    15061 aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc gaaaaagaga
    15121 taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat acattgttcg
    15181 taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa atataattca
    15241 attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc ccgtcgattg
    15301 caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc gcaaagcgcg
    15361 gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg tttggatcta
    15421 agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc acttgttttg
    15481 gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa gaaggtgttg
    15541 tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt gaggaagtcg
    15601 gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga gacacagtag
    15661 attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc tcttttgttg
    15721 tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct gctgttgcat
    15781 tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg atacataaga
    15841 ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt tgtctctgag
    15901 aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat ggttcttgtg
    15961 tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta cctcctaaac
    16021 tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc aatgttccat
    16081 gtttttttta aataaattta gtaaaatcgt tccccaatga agttgttcag tcttagaaaa
    16141 aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac aattctgcgg
    16201 cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc agttttcttc
    16261 attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct gacgtttgag
    16321 ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc attttttttg
    16381 cagctcatca aagggagctt gctctgtggc atattccaaa taaagctcaa aactttgtcg
    16441 ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac tttgataaaa
    16501 aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt gtacatagtt
    16561 ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt tatcgatccc
    16621 cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa actgctgttg
    16681 aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg taaaacgttc
    16741 cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa tgattgccgc
    16801 tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg ttttccgaac
    16861 aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg gtcgatcaaa
    16921 tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta gtgccagtgt
    16981 gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg gacggaaatc
    17041 aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa tattttgttt
    17101 tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa acaaaaattt
    17161 atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta aactattgaa
    17221 agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt ccattagaga
    17281 aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca attgatagca
    17341 aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat caagcaacgc
    17401 ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt tttttaattc
    17461 atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac aaacgaaatt
    17521 ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct cgcacggaat
    17581 ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg tatggaaaat
    17641 catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt tttaaatgac
    17701 ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg gtgatcatca
    17761 tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa agcgaatctg
    17821 gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc agataaaccg
    17881 ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc tcatttccag
    17941 ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac agctgcacca
    18001 aactctcata cgcagtatca atcaaaattt cacgtggcaa ttggctttca ctaagccatt
    18061 tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct tgaatacccg
    18121 ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta gtttcccctg
    18181 ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg gacaacatat
    18241 cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa ccttgctgcg
    18301 caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa ctgttatgga
    18361 taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt tttcccacaa
    18421 ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc gataaaataa
    18481 aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac tatgtaaact
    18541 tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag atattttcga
    18601 ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc aacagttcgt
    18661 gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag ttcgagcgat
    18721 tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac taagttctct
    18781 aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga cttattttga
    18841 aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct ataggaccca
    18901 tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca cagtttgctg
    18961 gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc tgccaaaacc
    19021 tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat actgcttagc
    19081 ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg ataaaacagg
    19141 aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga agaatacccc
    19201 ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta cgccacctaa
    19261 tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct tatccgcttt
    19321 gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg aaaagccgtt
    19381 ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag caccaggata
    19441 aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa ataagtaatc
    19501 ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt cgtacgtatt
    19561 ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga ctaagccaat
    19621 tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg accccttttt
    19681 cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact ttccagcatc
    19741 aaataaaaaa agacggaata tccatttgaa tatccgccct ttttgtatgt ttatttttat
    19801 ttagcacata aacgatacac agcttctgca taaatttcca ttgctttata catactactt
    19861 aattcccatc gttcattcgc ctggtgcata aagtcaggag tatcagggaa catcgcaccg
    19921 aatgccacac actggttcat cgtacgagca aaagtagcgc cgcctgatac aaacggttca
    19981 gtcatgtctc cagtaatatc gcgatatgtc cccaatagat ttttcactaa ctcactgtct
    20041 agtggcacgt ataatgaatc aaggaagtca aattcttcat aagtcaaccc atatttttct
    20101 gctgttttag ttaatttagc tactaaatca tctttcttga atgtaacagg gattctcata
    20161 tctacaccga tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt catcgttagc
    20221 tcgccggatt gttcatcttc tgttttgccc acgacagctt caccagttgc attttcttga
    20281 actaattttc ctaagaaatt gattgggcca aaatcaaata cttctgaaag agcaattgcc
    20341 agacgagaaa tagcattcac tccttgtgct gcatctttcg cgtggatact tttaccaaga
    20401 acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa tgcttctttt
    20461 acttcagaaa gcttttctcc agagtaagga gctgcatcag gaacaacatt caacgctcct
    20521 ccagctttta cagaaaactc gttcgttcct ggtcctgtca agtaagcttg caataagcct
    20581 ttttctgcat aaattaaagg aaattcagca tctggcgcaa atccttgcgt aattccttct
    20641 tctttttcgt tatatttttc taaacaacgc cataaatttt cttcatctgt accaaagata
    20701 aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca aagctttgac ggcatacatt
    20761 gccgcaatag aaggaccttt gtcatcttgg gatccgcgac caactaacca gccgtctttt
    20821 atagttggat cgaatggttt tgtttcccaa ttattttcgt cccctgctgg aacaacgtcc
    20881 atatgacaca agataccgaa taattcatct ccagagccaa tctctgaata accgtaatag
    20941 ccttctggat ctttaaatgt tctaaatcct agcttttcac tgatttccaa tactttatcc
    21001 aaagcttcaa tcactttttt accgaaggga tgtccttgtc ctgaatctgc ttcatctagt
    21061 acggaaggaa ttcggatcaa ttcgctcaat gattctaatg cttgttcatg atgttgttct
    21121 gtcacaaatt ttttcatgat attatcctcc taatatgtta aaaagggtca tgtcaaattc
    21181 ctccgacaca acctcttctt ctcaaataat gacgttttaa gcctgctcca tcagcaagca
    21241 gaatgttctt aacaaatgat gaaagaaaaa acactcactg aatggagcta ttgacttatc
    21301 cacgtgctta tcgatagccg tttatcggtt tcttcattct tatgatagac cttgtacctg
    21361 tatttgtcta ggtttagaac attgctgcta cacctaggat cacacagatg gctacaaata
    21421 atatcgcaat taatttaccg gtaaatttcc accacactga aacatcgaaa cgagcaattg
    21481 ctacagcacc cattacgata gcagatgttg gtgtaattaa gtttactaaa ccagatgcag
    21541 attgataagc agtgattact aaatccttac cgacgccggc aaattcaccc ataggtccca
    21601 tgattcccat tgttgcagca gctaagccgg aagttgaagg aatcaagaat gacataggga
    21661 tatagaaaat aaatgtaata atgatgaata cacttgaaga taatgaactt aatccttgtt
    21721 cgccccaatg taagatggtg tctgtgatta atccattgtt cataaccact tggataccac
    21781 gagcaacagc tactacgatt gctacaccga tcaaatcaga agcaccagct aaaaattcag
    21841 agacaaattc agactctttc atgccataag tgattgctac tacgatggac attactaaga
    21901 ataacatagt aatttctggg aagtaccagt cacctaatgg aagcatgctt ttacctagaa
    21961 ccgtacctag taccggaagg tttgttaacc agtttgtcaa tgattcaaaa atcgtgaaat
    22021 tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa gtaataaaga
    22081 acaatacatt aacatgtttt tgttttttcg tgattacttc ttgacgaccg attgcttcaa
    22141 tatcgaaatg cttcatatct tctttacgtt ggttgtatac taatgatttt gttggatctt
    22201 tttcgatctt tgatgcataa cgatacacat atacaataga aatagctaac aaaataacga
    22261 acatcaagaa tcgaagacca ataccttcac ccatcgaaat cccaacagct tgtgaagcta
    22321 cgcctgttgc aaaaggattt accgttgaag ctagacagcc tacttgagac cctactaata
    22381 cgattgctac agctactact gtatcaaagc ctacagccat catgactggg atcagtaaag
    22441 catagaatgg aatcgtttct tctgccattc cgtaagtaga gccgcctaat gcaaataaca
    22501 gcatcaatac tggaataagc attttttctt ttcctttgaa tttcttcaca actgaagcaa
    22561 tcccggcatc tagtgcccct gttttgttga taatacctaa aaatccacca acaaccaaaa
    22621 tgaagaaaga gattgggatt gcagcaggtg tttctttagt tagctccccc gctggtacac
    22681 ctagcatccc attgattggc gccatgaaaa tatcccacag cccttgagga ttttgttgta
    22741 ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc tacactatag tgcccagctg
    22801 gtataaacca agtaagtaca gcgattactg caataataat aaataagacc gtatacgctg
    22861 aaggcatttg aaattttttc ttttttgttt gttccatctt cttcttcctt tcttttccgt
    22921 tagagacaac aattttggct ctaatgttat tttatcaaaa agagtatcag aacaaaaata
    22981 ataacacgca ttttgtaaaa cttttgttca aaaagttcgt ttttccctat tccctcctct
    23041 tttttcaggt ttttaaagct ttcttctttt tcattgtagc gtttcctttt ttttataaac
    23101 aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt gattgatttt
    23161 tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat tattgcatgt
    23221 cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg atatttattt
    23281 gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg gaagaagttg
    23341 cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt tgaaaggaac
    23401 gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg cgttcagccc
    23461 taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca taaaaatagt
    23521 tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat gctgtttctt
    23581 tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga aactagcagt
    23641 gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata ctgaacgcaa
    23701 gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca ataccgagat
    23761 atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag caaaatactg
    23821 taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta cacgggtgtt
    23881 cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac gtttctctcc
    23941 aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat caagcgtcgt
    24001 caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt tcaccgattc
    24061 agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat ttcaccctgc
    24121 ccctgaagac gaaccttata taatttttgt ttacatattc attatacata tctattcaaa
    24181 aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt cagcaaactg
    24241 ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg taaagtcaga
    24301 tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg aagcatattt
    24361 ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg gtttgtctta
    24421 ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc gatctttatt
    24481 ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt tgcaacgtaa
    24541 actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc atcatttgtc
    24601 agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat acgttctttt
    24661 accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac agcagaaata
    24721 actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat tgggacttca
    24781 tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac aacttgtccg
    24841 tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga agtgatgcca
    24901 gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta ttcattccat
    24961 caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct tgtgtatcag
    25021 gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt gttccatata
    25081 atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta tgggcagcta
    25141 gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca gctcttgtga
    25201 ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat aatgtgtttg
    25261 atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc gttaatacat
    25321 aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg taagattctt
    25381 ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt ccgcttaatt
    25441 cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt tcacttaatt
    25501 tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata gttttctttt
    25561 tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa agtagtgaca
    25621 acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg cgcatttctt
    25681 tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc gggcgtgatc
    25741 gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat ggcacggatc
    25801 aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc taacccatcg
    25861 atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc tgtcagtctt
    25921 tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc ttctcgagga
    25981 tcttcttccc ataattcttc atcgtctgtg acgaattctt ggacaggaag caatgtcttg
    26041 cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac agctagctcg
    26101 agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc gatcggtata
    26161 tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag aggaccctcg
    26221 aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc tttttctttg
    26281 ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag acatccgggt
    26341 acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga taagacggat
    26401 tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata ccaataatcc
    26461 cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg tcttcagatt
    26521 cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta tggaaactca
    26581 atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc ttctcacctt
    26641 cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt ttcttcgtga
    26701 tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc tgtactgtcc
    26761 gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc gtatgtggtg
    26821 ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc cagattcctt
    26881 gccgagaaag acctttccca tgattattga caaatacatg catttcagat gcgtccttta
    26941 caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca taatcaccta
    27001 aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca agagaaagat
    27061 gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta gcatacatga
    27121 cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt gcttcaatca
    27181 gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc ttcggcgtat
    27241 cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac tgatggaatc
    27301 ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct tcatgcaact
    27361 tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca attttctgct
    27421 cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt gtattggcgg
    27481 acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct ttcataataa
    27541 tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc tttttccata
    27601 atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt tttctttttc
    27661 atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta aaaaatgata
    27721 acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt cctcgatcgt
    27781 gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt ttctcaaatc
    27841 ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt ctgtcaagat
    27901 ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt tcactaaaaa
    27961 gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa gcagagtagc
    28021 ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg tttttttcat
    28081 cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata attacaaata
    28141 aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga aaaacatgca
    28201 agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg tgtaatcaat
    28261 ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat accaaaagct
    28321 ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc tgcagaacgt
    28381 tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc tcttggcttc
    28441 aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt tatctgctcg
    28501 cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata gaaatcttcc
    28561 gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac atctttgttt
    28621 ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc tgctaaactg
    28681 ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc ccctttttta
    28741 ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc tttatctgga
    28801 agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat tccaaaagcg
    28861 taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg tttttgattt
    28921 tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga tttgcttaag
    28981 ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc aataataata
    29041 cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag tgaaaggttt
    29101 tcttaataca tttctttgct atcataacat aatgttatct acttgaatag ctgagccttg
    29161 gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc tttttcgaat
    29221 aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta attgctccca
    29281 tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg caaagcactt
    29341 tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt ataatagcct
    29401 tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat atatccacgt
    29461 tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca tgctggatta
    29521 gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc tgagtttgaa
    29581 gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc agcttgttga
    29641 gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc agctgctttt
    29701 ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc agtgagcatc
    29761 ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt tttcactttt
    29821 tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac tacttttccg
    29881 tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata ttgattttta
    29941 gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt ttcgattgct
    30001 tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc gacgatggca
    30061 ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg gatatcttgg
    30121 taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa atcatttttt
    30181 agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg atcatctgta
    30241 taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt tgcttctgct
    30301 gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc tccaccgagt
    30361 agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt ttgcgcttta
    30421 tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc tggcacgatt
    30481 gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt ttctattttt
    30541 gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga ttcactattt
    30601 atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt tttctctgct
    30661 tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac ttcagttttt
    30721 tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt ggcgttttct
    30781 ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt ttcctgtgca
    30841 attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt atcttcacga
    30901 tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac gatttctttc
    30961 agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt ctttcctgat
    31021 tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat attagttact
    31081 tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa ttccattaca
    31141 gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa ttctgtatca
    31201 ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt tgtttcttca
    31261 attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc tggacagata
    31321 gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac ttagcgatcg
    31381 gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt cacgccaata
    31441 gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata gaaaaaaaag
    31501 cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca gttttactga
    31561 tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg ttttgaacga
    31621 gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg ttcccgagta
    31681 acaccaaata ctttccctac ttcttccaat gttctcgtcc ggccatcatc taaaccaaaa
    31741 cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc ttccaattgt
    31801 tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg gtcttctatg
    31861 aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga aacaggttct
    31921 tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc cgcaccgatt
    31981 tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat acgtatcaat
    32041 ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg gtcagcgatt
    32101 gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt aaatcctttg
    32161 cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg gatcaagtca
    32221 aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa acgaaggttt
    32281 gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa tgctaacgct
    32341 acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata catacgaacc
    32401 gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc ttgttcagct
    32461 tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc caccacactg
    32521 atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt caaagtgaat
    32581 ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg tttgttttct
    32641 ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt tgccataaaa
    32701 gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa ttgatgattt
    32761 caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct tgctgcattt
    32821 gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct agcaagtctt
    32881 ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc accaccaacc
    32941 gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat tcataatgtt
    33001 gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga ataaactgaa
    33061 attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga tataatagaa
    33121 tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt tcttgtctcg
    33181 taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt gtccttcgaa
    33241 gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt gacagttgag
    33301 atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact agtaattctt
    33361 cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt aaataacgtg
    33421 atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa gatgcttctc
    33481 catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg acagaaagtg
    33541 ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca aaacctgcgt
    33601 tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt tggatctgtt
    33661 gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc tgccatgcag
    33721 caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg atatcgcttc
    33781 ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt tctggactat
    33841 tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag cgtcctgaaa
    33901 aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga taaaaacgat
    33961 cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac aattcatttg
    34021 aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt gcaaaaccta
    34081 tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat tctaaagctg
    34141 gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct ttttcatgag
    34201 cagcaatcag tttccccgta gcagattcag gattttggac agctatagta cgctgacgcc
    34261 attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct tctggaaaac
    34321 ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca caaccaaagc
    34381 aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct tcatgaaaag
    34441 gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt ccaatcacct
    34501 caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc cgttgagcca
    34561 ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat ttcaggagaa
    34621 taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca caattataca
    34681 tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta tctcattaag
    34741 catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca gtacaatact
    34801 cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc tcccaaccct
    34861 aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc tacgatcgtc
    34921 gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac aactgccatg
    34981 caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt tccccatgcg
    35041 aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa ggcatttggt
    35101 aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt atcttgttca
    35161 aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt gatttggata
    35221 gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat aaatgaataa
    35281 atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac tcggcgtcct
    35341 tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag attcgcaatc
    35401 tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc tcttaacgcc
    35461 aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc tttcaattca
    35521 tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata gcgatgagca
    35581 gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc atcataattc
    35641 ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc agaaatcact
    35701 ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt ttttcggatt
    35761 ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc ttcgttcaat
    35821 tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg ggctgttttt
    35881 agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac gtacacgact
    35941 cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc gactttgttt
    36001 gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat agtgatgttg
    36061 ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt ctcgtacagc
    36121 ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt ccctgtttta
    36181 tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc ttgaacaagg
    36241 attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat tagcaatacc
    36301 aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga aattttgata
    36361 gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg aataatcacg
    36421 aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg aatagctttg
    36481 tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac tcggataact
    36541 tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc tccagtttct
    36601 ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc ccgtccagat
    36661 ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa atctcctaat
    36721 accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag tacttctgat
    36781 tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg ataaattcca
    36841 ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc acctacaaat
    36901 aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat attatcgctg
    36961 aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg gccagaagcc
    37021 attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc cattttccgc
    37081 caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa aaacaacttg
    37141 tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg ccaacctttt
    37201 tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat attgttcctc
    37261 caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag gacaagctag
    37321 taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg ttgaagcaaa
    37381 aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa tgtaatccat
    37441 gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca caatcacttc
    37501 aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat acaccaaaaa
    37561 attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc cggcaatcag
    37621 ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt ataataaaaa
    37681 caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat ctcttccttt
    37741 tttccctgct tctttccata acgcaggaac agttccagcg atacatccga caaaaaacca
    37801 taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa gtagaaagat
    37861 gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt tgaaattttt
    37921 tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag ctgctaatgc
    37981 accaccgctg actcctggca agataaatcc tgaaccgata aacattccct tcacaaaccg
    38041 taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt gccttttttc
    38101 cactatttgc gtactccttc tttcatttaa ttttattcat gattgaacat ttcctgctat
    38161 tttacttaaa gactttttca taggtctcta ttattttttc attcattttg acaatatcag
    38221 gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc aaggactgga
    38281 tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc agcacatctt
    38341 tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt acataggcaa
    38401 tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat ccttgtttct
    38461 ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg tcagctggat
    38521 tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga tctgtccctt
    38581 ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt tccatatgat
    38641 aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt accgaacgat
    38701 aagttcttcc tcataaaaac aacaccgcac caataaccgt cagaatgatc atgtaaaaaa
    38761 gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat tttgacatct
    38821 ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt ctgtgtacgc
    38881 cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga tcgttgtttt
    38941 tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca gtaggtgatt
    39001 atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt tactatgaaa
    39061 agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc ggccgctttt
    39121 tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta gatagccgag
    39181 gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg gccgaggatc
    39241 tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga tttagtgatg
    39301 gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat cgattgattt
    39361 taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc atcagtaata
    39421 ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc aaaaatcttt
    39481 tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta aaaaaaatca
    39541 actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa cattctttgt
    39601 atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa ggacatgaat
    39661 tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt tctgaaacat
    39721 aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg gctgaaaaac
    39781 cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat tgtcggtgaa
    39841 ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt tgccactata
    39901 cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt acctatggtt
    39961 ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg ttacctgttt
    40021 gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg gtttgttagt
    40081 gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt gatccaagca
    40141 ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct gctttctttt
    40201 ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat atggttgatc
    40261 ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa gaaaggattc
    40321 cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt gttggaatgg
    40381 accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg gattgatctg
    40441 tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc tatgattcca
    40501 ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat tggtgtgcca
    40561 agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat cattccgttt
    40621 ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca gcgttattct
    40681 ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt cttattatac
    40741 ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac agagtttcgc
    40801 tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc tagtatacta
    40861 ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa acgagcatat
    40921 ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag tgattttagt
    40981 ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc taaaaacgaa
    41041 ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg aatcattatt
    41101 ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccgga tttccctcat
    41161 cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg gttttcggga
    41221 ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt ccttcaagga
    41281 gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat ccttacaact
    41341 tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat gtttgcctat
    41401 gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa taaatgtatt
    41461 gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga agcttttatc
    41521 gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga agagattgtt
    41581 atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc ttatgtagac
    41641 ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt cttgaatcgg
    41701 atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac tgagcaaatg
    41761 agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa agggttttct
    41821 ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt caaaaatacc
    41881 caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa taaagtagga
    41941 acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat ggatttcttg
    42001 ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa tttagggatg
    42061 gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag gattgcatac
    42121 ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga atataaaagc
    42181 aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag ttggattgca
    42241 tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac acaaggtaga
    42301 ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac cgatagatga
    42361 acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct tctacaatct
    42421 cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt ggaccgctct
    42481 tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat gcttcgatct
    42541 ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat gtcgttccgt
    42601 tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca ccaggcatga
    42661 ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg cggaagtaga
    42721 actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct tttgtcaaca
    42781 cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta gcaagtactt
    42841 gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg ttatccccag
    42901 cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact gctttttgtg
    42961 tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca ccacgatcaa
    43021 tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc actggcaata
    43081 gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact gtatccatca
    43141 attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat gcggaacctt
    43201 tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg cgaacttcca
    43261 tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc aagaaaacaa
    43321 tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt tgaggcatcg
    43381 gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc cctgtaatca
    43441 tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg cgtttttctg
    43501 tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct tctggagctg
    43561 catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat actgtagtga
    43621 tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca atgttaacgt
    43681 gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct aaataatata
    43741 gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga ctaaatcaaa
    43801 aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata cgtcaatatt
    43861 ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac cttttataaa
    43921 taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct tttgaagcaa
    43981 taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt caatagacga
    44041 aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa ttaattggcg
    44101 tacttgtcgt tttaggcata tttctcggcg gaagttt
//
LOCUS       DAYYNQ010000010        43103 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000010 DAYYNQ010000000
VERSION     DAYYNQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.010"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBJ1_000526"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHBJ1_000526"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM9429498.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBJ1_000527"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHBJ1_000527"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM9429499.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBJ1_000528"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHBJ1_000528"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM9429500.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHBJ1_000529"
     CDS             2179..2499
                     /locus_tag="ACHBJ1_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM9429501.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="ACHBJ1_000530"
     CDS             complement(2611..3480)
                     /locus_tag="ACHBJ1_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9429502.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHBJ1_000531"
     CDS             complement(3576..4880)
                     /locus_tag="ACHBJ1_000531"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9429503.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHBJ1_000532"
     CDS             complement(4877..5386)
                     /locus_tag="ACHBJ1_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429504.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHBJ1_000533"
     CDS             complement(5561..7696)
                     /locus_tag="ACHBJ1_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM9429505.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHBJ1_000534"
     CDS             complement(7779..9434)
                     /locus_tag="ACHBJ1_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9429506.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHBJ1_000535"
     CDS             complement(9436..9750)
                     /locus_tag="ACHBJ1_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9429507.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHBJ1_000536"
     CDS             9920..10180
                     /locus_tag="ACHBJ1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM9429508.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHBJ1_000537"
     CDS             10194..10508
                     /locus_tag="ACHBJ1_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM9429509.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHBJ1_000538"
     CDS             complement(11238..11792)
                     /locus_tag="ACHBJ1_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM9429510.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHBJ1_000539"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHBJ1_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHBJ1_000540"
     CDS             complement(14153..14869)
                     /locus_tag="ACHBJ1_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9429511.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBJ1_000541"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHBJ1_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM9429512.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBJ1_000542"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHBJ1_000542"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM9429513.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHBJ1_000543"
     CDS             complement(18339..19799)
                     /locus_tag="ACHBJ1_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429514.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHBJ1_000544"
     CDS             complement(19789..21084)
                     /locus_tag="ACHBJ1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9429515.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHBJ1_000545"
     CDS             complement(21416..22093)
                     /locus_tag="ACHBJ1_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM9429516.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHBJ1_000546"
     CDS             complement(22123..23214)
                     /locus_tag="ACHBJ1_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM9429517.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHBJ1_000547"
     CDS             complement(23315..23749)
                     /locus_tag="ACHBJ1_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9429518.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHBJ1_000548"
     CDS             complement(24333..25790)
                     /locus_tag="ACHBJ1_000548"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9429519.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHBJ1_000549"
     CDS             complement(25787..27682)
                     /locus_tag="ACHBJ1_000549"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9429520.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHBJ1_000550"
     CDS             complement(27740..29515)
                     /locus_tag="ACHBJ1_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9429521.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHBJ1_000551"
     CDS             complement(29565..31061)
                     /locus_tag="ACHBJ1_000551"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9429522.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="ACHBJ1_000552"
     CDS             complement(31255..32877)
                     /locus_tag="ACHBJ1_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9429523.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="ACHBJ1_000553"
     CDS             complement(33205..34518)
                     /locus_tag="ACHBJ1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9429524.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="ACHBJ1_000554"
     CDS             complement(34763..34987)
                     /locus_tag="ACHBJ1_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429525.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="ACHBJ1_000555"
     CDS             complement(35007..35429)
                     /locus_tag="ACHBJ1_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9429526.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="ACHBJ1_000556"
     CDS             complement(35571..36281)
                     /locus_tag="ACHBJ1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM9429527.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="ACHBJ1_000557"
     CDS             complement(36299..36796)
                     /locus_tag="ACHBJ1_000557"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429528.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="ACHBJ1_000558"
     CDS             complement(36833..37285)
                     /locus_tag="ACHBJ1_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9429529.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="ACHBJ1_000559"
     CDS             complement(37420..38025)
                     /locus_tag="ACHBJ1_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9429530.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="ACHBJ1_000560"
     CDS             complement(38475..39095)
                     /locus_tag="ACHBJ1_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9429531.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="ACHBJ1_000561"
     CDS             complement(39097..39966)
                     /locus_tag="ACHBJ1_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9429532.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="ACHBJ1_000562"
     CDS             complement(39989..40396)
                     /locus_tag="ACHBJ1_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM9429533.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="ACHBJ1_000563"
     CDS             complement(40415..41704)
                     /locus_tag="ACHBJ1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9429534.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            41762..43101
                     /locus_tag="ACHBJ1_000564"
     CDS             join(41762..42203,42203..43101)
                     /locus_tag="ACHBJ1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM9429535.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        12342 a         9165 c         7265 g        14331 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgttttg atgtgcaccc caaaagttag actagaaatc taattaaaag gggtgctttt
    41761 tttgcggaaa tatacatttg tttttaagaa aaaagtagtt tcagactatt taaataacga
    41821 aggcggctac aaatatcttg cacataaata tcaaataaat cgtacactgg ttagacattg
    41881 ggtaaggatt tataactatc atggttggga aggcttggtt ggaggtggca aaagctacac
    41941 tacaaaattt aaacttgatg ttatagaata tatggaaaca aatggtcttt ctatccaaga
    42001 aactgctaaa aaatttaata tcggttcaaa tagaactcta agtaaatgga tagagcaata
    42061 tgaagaaggc ggtgcttctt cacttgagag ccaaaaaagg ggcagaaaaa ttagtatgaa
    42121 ttccaagcta aacattccta aaaaacttaa agatgagtct cttgaagaag aagttattcg
    42181 tttaagagca gagaacgcat atttaaaaaa gttagaaacc ttgattcaag aacaagattt
    42241 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
    42301 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
    42361 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
    42421 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
    42481 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
    42541 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
    42601 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
    42661 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
    42721 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
    42781 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
    42841 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
    42901 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
    42961 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
    43021 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
    43081 cggaaacagt ccattaaata gtt
//
LOCUS       DAYYNQ010000011        40671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000011 DAYYNQ010000000
VERSION     DAYYNQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.011"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1185
                     /locus_tag="ACHBJ1_000565"
                     /pseudo
     CDS             44..1185
                     /locus_tag="ACHBJ1_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1311..2390)
                     /locus_tag="ACHBJ1_000566"
     CDS             complement(1311..2390)
                     /locus_tag="ACHBJ1_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9429536.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2493..2933)
                     /locus_tag="ACHBJ1_000567"
     CDS             complement(2493..2933)
                     /locus_tag="ACHBJ1_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9429537.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2990..3400)
                     /locus_tag="ACHBJ1_000568"
     CDS             complement(2990..3400)
                     /locus_tag="ACHBJ1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9429538.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3580..4014)
                     /locus_tag="ACHBJ1_000569"
     CDS             complement(3580..4014)
                     /locus_tag="ACHBJ1_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9429539.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4114..4962)
                     /locus_tag="ACHBJ1_000570"
     CDS             complement(4114..4962)
                     /locus_tag="ACHBJ1_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9429540.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5351..7117
                     /locus_tag="ACHBJ1_000571"
     CDS             5351..7117
                     /locus_tag="ACHBJ1_000571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM9429541.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7219..8478
                     /locus_tag="ACHBJ1_000572"
     CDS             7219..8478
                     /locus_tag="ACHBJ1_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9429542.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8625..9926
                     /locus_tag="ACHBJ1_000573"
     CDS             8625..9926
                     /locus_tag="ACHBJ1_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429543.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9929..10780
                     /locus_tag="ACHBJ1_000574"
     CDS             9929..10780
                     /locus_tag="ACHBJ1_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9429544.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10793..11608
                     /locus_tag="ACHBJ1_000575"
     CDS             10793..11608
                     /locus_tag="ACHBJ1_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM9429545.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11759..12925
                     /locus_tag="ACHBJ1_000576"
     CDS             11759..12925
                     /locus_tag="ACHBJ1_000576"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9429546.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13155..13802
                     /locus_tag="ACHBJ1_000577"
     CDS             13155..13802
                     /locus_tag="ACHBJ1_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM9429547.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13886..14830
                     /locus_tag="ACHBJ1_000578"
     CDS             13886..14830
                     /locus_tag="ACHBJ1_000578"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM9429548.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14965..16416
                     /locus_tag="ACHBJ1_000579"
     CDS             14965..16416
                     /locus_tag="ACHBJ1_000579"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM9429549.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16437..17273
                     /locus_tag="ACHBJ1_000580"
     CDS             16437..17273
                     /locus_tag="ACHBJ1_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9429550.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17273..17932
                     /locus_tag="ACHBJ1_000581"
     CDS             17273..17932
                     /locus_tag="ACHBJ1_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9429551.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18058..18747
                     /locus_tag="ACHBJ1_000582"
     CDS             18058..18747
                     /locus_tag="ACHBJ1_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429552.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18753..19709
                     /locus_tag="ACHBJ1_000583"
     CDS             18753..19709
                     /locus_tag="ACHBJ1_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM9429553.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19753..20265
                     /locus_tag="ACHBJ1_000584"
     CDS             19753..20265
                     /locus_tag="ACHBJ1_000584"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429554.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20521..21069)
                     /locus_tag="ACHBJ1_000585"
     CDS             complement(20521..21069)
                     /locus_tag="ACHBJ1_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9429555.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21158..22339)
                     /locus_tag="ACHBJ1_000586"
     CDS             complement(21158..22339)
                     /locus_tag="ACHBJ1_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM9429556.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22575..24083)
                     /locus_tag="ACHBJ1_000587"
     CDS             complement(22575..24083)
                     /locus_tag="ACHBJ1_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM9429557.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24242..24433
                     /locus_tag="ACHBJ1_000588"
     CDS             24242..24433
                     /locus_tag="ACHBJ1_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429558.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24636..25553
                     /locus_tag="ACHBJ1_000589"
     CDS             24636..25553
                     /locus_tag="ACHBJ1_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM9429559.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25546..26619
                     /locus_tag="ACHBJ1_000590"
     CDS             25546..26619
                     /locus_tag="ACHBJ1_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429560.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26767..27285
                     /locus_tag="ACHBJ1_000591"
     CDS             26767..27285
                     /locus_tag="ACHBJ1_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429561.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27266..27658
                     /locus_tag="ACHBJ1_000592"
     CDS             27266..27658
                     /locus_tag="ACHBJ1_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM9429562.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27734..28669
                     /locus_tag="ACHBJ1_000593"
     CDS             27734..28669
                     /locus_tag="ACHBJ1_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9429563.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28802..29581
                     /locus_tag="ACHBJ1_000594"
     CDS             28802..29581
                     /locus_tag="ACHBJ1_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM9429564.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29574..29891
                     /locus_tag="ACHBJ1_000595"
     CDS             29574..29891
                     /locus_tag="ACHBJ1_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM9429565.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30135..31745
                     /locus_tag="ACHBJ1_000596"
     CDS             30135..31745
                     /locus_tag="ACHBJ1_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9429566.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31902..32750
                     /locus_tag="ACHBJ1_000597"
     CDS             31902..32750
                     /locus_tag="ACHBJ1_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9429567.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            32981..33460
                     /locus_tag="ACHBJ1_000598"
     CDS             32981..33460
                     /locus_tag="ACHBJ1_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM9429568.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33493..35721
                     /locus_tag="ACHBJ1_000599"
     CDS             33493..35721
                     /locus_tag="ACHBJ1_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9429569.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35850..37493
                     /locus_tag="ACHBJ1_000600"
     CDS             35850..37493
                     /locus_tag="ACHBJ1_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM9429570.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37566..38111)
                     /locus_tag="ACHBJ1_000601"
     CDS             complement(37566..38111)
                     /locus_tag="ACHBJ1_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM9429571.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38134..38619)
                     /locus_tag="ACHBJ1_000602"
     CDS             complement(38134..38619)
                     /locus_tag="ACHBJ1_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9429572.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38609..39421)
                     /locus_tag="ACHBJ1_000603"
     CDS             complement(38609..39421)
                     /locus_tag="ACHBJ1_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM9429573.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39560..>40671)
                     /locus_tag="ACHBJ1_000604"
     CDS             complement(39560..>40671)
                     /locus_tag="ACHBJ1_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM9429574.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13497 a         7378 c         7961 g        11835 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagaca gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc
      481 gtatagtcag cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt
      541 tgaagaacca acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa
      601 tccagacgga gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca
      661 accagagcca gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac
      721 tccagaacca gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga
      781 taatgaaact gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac
      841 tccagaacca gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac
      901 aattcaagag gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa
      961 tgaagagcag ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt
     1021 aaaaagtagc aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa
     1081 tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca
     1141 acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc
     1201 tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt
     1261 gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt ttaagggata
     1321 tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag
     1381 gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt
     1441 aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc
     1501 agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct
     1561 cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct
     1621 gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag
     1681 ccaaatacag ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga
     1741 gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga
     1801 aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt
     1861 tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct
     1921 atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg
     1981 tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc
     2041 tcgtaaaaat taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata
     2101 tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa
     2161 acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa
     2221 gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa
     2281 acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa
     2341 acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg
     2401 aatatgaaaa taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg
     2461 gttttcctca taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa
     2521 ttatctagca tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga
     2581 agataagact tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga
     2641 gcagtcagcg taacatttac gattctttca tcacccgttt cacgctgacg attaatataa
     2701 tttttttctg ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca
     2761 tttcctagct ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac
     2821 tgggtatagg taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta
     2881 gaggcggcat ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca
     2941 cctccgctgt tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa
     3001 ctgctttcag agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat
     3061 gcgctgcctc taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa
     3121 tctctacacc taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga
     3181 cagtagaagc cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac
     3241 atgcactgaa accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac
     3301 caggagctgc aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc
     3361 cgcctgtgtt gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta
     3421 tattttatac aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca
     3481 taagataaat aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac
     3541 aaaaaatatc agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag
     3601 ttgttttctt tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc
     3661 aaaaggatct ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg
     3721 aatctctaca cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat
     3781 gatattgaac cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt
     3841 gttttcatcc agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc
     3901 tctagggtaa tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc
     3961 tagtgctttc aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat
     4021 ctcctctcct tttcagtcga acagttacta gattctttta ttataccagc taaagaaaac
     4081 gcattcaaaa aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc
     4141 gcgtcccatt acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc
     4201 tatcaatggt ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt
     4261 tgactttcga ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc
     4321 acttaagcga aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt
     4381 tcgattgata cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat
     4441 agcataaaga aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc
     4501 tgtaaaaaat aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg
     4561 gatcgtcgta ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa
     4621 aatcgcactt ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac
     4681 tattgcaatg attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt
     4741 acctaaaaat agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc
     4801 tcggtcaaag cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc
     4861 aatatgatga ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat
     4921 aatcttaatg tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt
     4981 cattaataat actcttatct tattataact tacttttcac cttaagttaa ctttaattaa
     5041 taaaaaaatt aggctagtct aaattattat gtttcattct tctagaatat acttctacgc
     5101 caaaaactgt taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa
     5161 ttcaggacac taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct
     5221 atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt
     5281 gaaagcgttg tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag
     5341 gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct
     5401 atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt
     5461 atatgtgcta tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg
     5521 accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag
     5581 cgaaacaagt cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt
     5641 ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta
     5701 ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga
     5761 aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc
     5821 cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga
     5881 tttgcaaggt gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta
     5941 tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg attattatga
     6001 ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa
     6061 aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca
     6121 gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg
     6181 ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc
     6241 atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt
     6301 gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg
     6361 gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt
     6421 tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg
     6481 gctgcaaggg gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga
     6541 ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca
     6601 aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac
     6661 tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt
     6721 tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg
     6781 tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga
     6841 gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc
     6901 tgaaggttct actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg
     6961 gaaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt
     7021 agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc
     7081 agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag
     7141 aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat
     7201 acaaaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc
     7261 tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg
     7321 gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata
     7381 aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag
     7441 gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac
     7501 gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa
     7561 gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat
     7621 ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat
     7681 ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt
     7741 tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt
     7801 tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca
     7861 aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca
     7921 gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc
     7981 gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg
     8041 caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat
     8101 ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa
     8161 aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa
     8221 ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct
     8281 aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca
     8341 aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca
     8401 tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc
     8461 atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt
     8521 gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc
     8581 ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa
     8641 caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt
     8701 ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg
     8761 gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac
     8821 caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa
     8881 tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc
     8941 gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg
     9001 aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt acgatcattt
     9061 tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat catattccac
     9121 cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct
     9181 catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa
     9241 caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta
     9301 aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta
     9361 ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga
     9421 ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc
     9481 cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct
     9541 atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa
     9601 tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag
     9661 tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc tccatgatct
     9721 atctgtttaa caatggcggt cccggaagcg taggcggagg agctggttct actgatattc
     9781 ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag
     9841 caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga
     9901 caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca
     9961 aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat
    10021 ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag
    10081 tttagatctt tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact
    10141 ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt
    10201 gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc
    10261 aagtctgatg ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt
    10321 ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta
    10381 tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc
    10441 aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca
    10501 gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg
    10561 accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt
    10621 tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc
    10681 tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa
    10741 ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac
    10801 atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa
    10861 actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat
    10921 gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc
    10981 cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga
    11041 aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt
    11101 gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca
    11161 atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc
    11221 atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga
    11281 attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt
    11341 cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag
    11401 aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt
    11461 tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt
    11521 gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac
    11581 aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt
    11641 gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt
    11701 tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat
    11761 gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt
    11821 aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa
    11881 aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgcat ctttggcact
    11941 aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa
    12001 cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc
    12061 aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt
    12121 aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt
    12181 caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga
    12241 caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt
    12301 gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga
    12361 acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt
    12421 attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg
    12481 tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt
    12541 attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac
    12601 aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt
    12661 agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga
    12721 ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac
    12781 gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc
    12841 ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa
    12901 tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa
    12961 agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc
    13021 tctaaagctt agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt
    13081 taaacatgac agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa
    13141 aggggatcga atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt
    13201 tagctgcttg tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg
    13261 aacagcgctc ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc
    13321 agacaaaaag ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg
    13381 cgatcgaatt gttccaagat aaatacccag atactgcaat tactagtttg gaattagata
    13441 gtgattgggg atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag
    13501 caagaattga tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag
    13561 atgaacaaaa tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga
    13621 ttgaggaagc agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg
    13681 atttggataa agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa
    13741 gtacatcagt caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt
    13801 aagaatttct tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa
    13861 taacttgttt gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt
    13921 tgccaatcct ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga
    13981 actggctcat tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa
    14041 aggaaatccc gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact
    14101 tccaaactta ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga
    14161 aaaccaaaat caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga
    14221 aatgcttgag aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg
    14281 tcccaatgtg cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact
    14341 gaaagaagta ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga
    14401 ttttgctcat tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa
    14461 tgctatcaac agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat
    14521 caaaccaaaa gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc
    14581 gaatgttcgc gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg
    14641 gattcggaca ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat
    14701 tggtaccgag cttcataaag aagggccgga aattttttct agaatcataa aagaattaac
    14761 gcagattatg tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac
    14821 catttcatag caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac
    14881 tcgtctgttc attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat
    14941 aacagaggca aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa
    15001 gaaaaagaat ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa
    15061 aatcatgaat tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta
    15121 ttgattcccc agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc
    15181 ggtcaagaaa aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga
    15241 gcttctcata catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga
    15301 caaaccaatg cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca
    15361 gcaagaaacc cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct
    15421 gcagtttcat caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga
    15481 atacccgctt cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt
    15541 ggtccatatg cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct
    15601 atgagagaca cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat
    15661 caagtatcgc cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt
    15721 tgtgttgatt caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa
    15781 gctgtagatt tccttggaaa agaagggcat gatttgacag agattcctta cccactagat
    15841 ggtcgtaaac tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc
    15901 cgctcgattg aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct
    15961 tggactttgt tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat
    16021 gtatgggatg aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt
    16081 tcaccaacga ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata
    16141 agagcacaga tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct
    16201 tccatgtttg aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga
    16261 caaccagcaa tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa
    16321 tttatggctg ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca
    16381 aatcatcagt tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga
    16441 agaaaaaaag attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta
    16501 cagctgccgc taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag
    16561 tagtagcgac ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag
    16621 ttggttatga tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct
    16681 ttaaagaata caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg
    16741 ctgcgaacga ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt
    16801 tcaaatattc atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac
    16861 ttgaagatct taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag
    16921 cggaaaaaca cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa
    16981 gcgatgtagc aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg
    17041 ccgtatctgc tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa
    17101 acgaaagtgg ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg
    17161 tattaaaaga gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa
    17221 ctgatgtttc agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt
    17281 tcctctaatt gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt
    17341 atacacatta gggattggct tcgtgtcgtt tcttttagga aatctggtag ggttgctttt
    17401 gacagtttta ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc
    17461 gttctttaga ggcattcccg cactagtcct gctgtttctg ttatattttg ggttacctta
    17521 tcaattaagt gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat
    17581 tggtgaaatc tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt
    17641 agcttctggt ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg
    17701 aatctcagta ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc
    17761 agcaacaatc acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt
    17821 cgattatttt tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat
    17881 cgaaaaagtg caacagtatt tagaaaaacg ttctatgcat cagtcaatct gaaaaataga
    17941 aatggtgcta gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt
    18001 attttaaaac catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg
    18061 aagaccccgc taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc
    18121 ggcttggttg tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc
    18181 tgcttgacaa aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa
    18241 gcagattttc gctctacagc caaactgtca tttgttggac attataatta ttttgtttta
    18301 ccaagtaaac tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg
    18361 tatcgtccgt acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa
    18421 aaagcagcaa agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct
    18481 tctttattca gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca
    18541 acagatcaat tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac
    18601 gaacgatatt atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa
    18661 gaagaagtta tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt
    18721 cggttgccta cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac
    18781 aatttgacct attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc
    18841 aaccagtgat cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc
    18901 aaaaaaagca ccatcgaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat
    18961 tcgatcagca tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga
    19021 tcttaacgcc agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg
    19081 atggacagca ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga
    19141 aatttttgat accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga
    19201 tccgtgactc ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact
    19261 tttttagtga tgactatctt tttttccacg cagacggata tattggattt tctgatttca
    19321 cgatcgaagg tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc
    19381 taacgtatat tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg
    19441 actctgctaa agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga
    19501 tcatgcgtca ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt
    19561 accagcaaca aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt
    19621 tggaacttat tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag
    19681 ttcaggaact atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa
    19741 agaggaaact ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa
    19801 atcattaatg ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg
    19861 cagaaaccaa caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta
    19921 atagtggaag atcaaattgc tggaacagct gcattagtct caagcgtgga tcccgtttac
    19981 acaaaaatag atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc
    20041 gctttagcag aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg
    20101 acgattgcag ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa
    20161 ccgatggaaa agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct
    20221 tttgaacacg gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg
    20281 agcaaagata aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg
    20341 taacagaagt ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt
    20401 ttgtggaatt ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag
    20461 gtcgtgacat agcttttgtc acgacctctc ggcttatcct acgagtatta ttcgatacat
    20521 ttatatcaat aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga
    20581 cacgactaaa agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata
    20641 aaccgccagt ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg
    20701 caatgtttct ttagagatcg taataaagat aggacggact accgtcccga aatactcata
    20761 taaatgccaa aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt
    20821 tttaatttct gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa
    20881 ctcatttccc ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg
    20941 atctttttcc ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg
    21001 aaaaatagct tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca
    21061 ataattcatt gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt
    21121 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
    21181 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
    21241 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
    21301 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
    21361 ggaaattacc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
    21421 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
    21481 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
    21541 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
    21601 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
    21661 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
    21721 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
    21781 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
    21841 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
    21901 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
    21961 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
    22021 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
    22081 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
    22141 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
    22201 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
    22261 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
    22321 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
    22381 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
    22441 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
    22501 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
    22561 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
    22621 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
    22681 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
    22741 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
    22801 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
    22861 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
    22921 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
    22981 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
    23041 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
    23101 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
    23161 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
    23221 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
    23281 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
    23341 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
    23401 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
    23461 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
    23521 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
    23581 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
    23641 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
    23701 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
    23761 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
    23821 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
    23881 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
    23941 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
    24001 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
    24061 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
    24121 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
    24181 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
    24241 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
    24301 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
    24361 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
    24421 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
    24481 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
    24541 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
    24601 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
    24661 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
    24721 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
    24781 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
    24841 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
    24901 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
    24961 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
    25021 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
    25081 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
    25141 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
    25201 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
    25261 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
    25321 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
    25381 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
    25441 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
    25501 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
    25561 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
    25621 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
    25681 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
    25741 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
    25801 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
    25861 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
    25921 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
    25981 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
    26041 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
    26101 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
    26161 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
    26221 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
    26281 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
    26341 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
    26401 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
    26461 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
    26521 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
    26581 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
    26641 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
    26701 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
    26761 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
    26821 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
    26881 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
    26941 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
    27001 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
    27061 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
    27121 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
    27181 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
    27241 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
    27301 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
    27361 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
    27421 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
    27481 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
    27541 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
    27601 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
    27661 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
    27721 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
    27781 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
    27841 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
    27901 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
    27961 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
    28021 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
    28081 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
    28141 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
    28201 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
    28261 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
    28321 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
    28381 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
    28441 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
    28501 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
    28561 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
    28621 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
    28681 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
    28741 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
    28801 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
    28861 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
    28921 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
    28981 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
    29041 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
    29101 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
    29161 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
    29221 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
    29281 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
    29341 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
    29401 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
    29461 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
    29521 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
    29581 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
    29641 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
    29701 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
    29761 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
    29821 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
    29881 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
    29941 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
    30001 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
    30061 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
    30121 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
    30181 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
    30241 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
    30301 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
    30361 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
    30421 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
    30481 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
    30541 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
    30601 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
    30661 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
    30721 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
    30781 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
    30841 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
    30901 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
    30961 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
    31021 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
    31081 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    31141 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    31201 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    31261 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    31321 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    31381 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    31441 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    31501 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    31561 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    31621 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    31681 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    31741 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    31801 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    31861 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    31921 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    31981 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    32041 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    32101 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    32161 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    32221 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    32281 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    32341 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    32401 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    32461 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    32521 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    32581 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    32641 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    32701 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    32761 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    32821 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    32881 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    32941 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    33001 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    33061 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    33121 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    33181 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    33241 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    33301 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    33361 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    33421 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    33481 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    33541 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    33601 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    33661 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    33721 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    33781 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    33841 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    33901 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    33961 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    34021 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    34081 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    34141 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    34201 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    34261 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    34321 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    34381 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    34441 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    34501 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    34561 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    34621 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    34681 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    34741 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    34801 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    34861 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    34921 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    34981 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    35041 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    35101 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    35161 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    35221 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    35281 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    35341 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    35401 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    35461 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    35521 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    35581 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    35641 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    35701 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    35761 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    35821 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    35881 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    35941 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    36001 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    36061 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    36121 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    36181 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    36241 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    36301 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    36361 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    36421 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    36481 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    36541 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    36601 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    36661 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    36721 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    36781 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    36841 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    36901 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    36961 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    37021 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    37081 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    37141 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    37201 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    37261 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    37321 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    37381 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    37441 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    37501 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    37561 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    37621 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    37681 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    37741 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    37801 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    37861 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    37921 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    37981 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    38041 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    38101 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    38161 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    38221 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    38281 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    38341 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    38401 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    38461 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    38521 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    38581 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    38641 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    38701 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    38761 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    38821 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    38881 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    38941 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    39001 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    39061 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    39121 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    39181 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    39241 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    39301 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    39361 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    39421 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    39481 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    39541 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    39601 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    39661 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    39721 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    39781 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    39841 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    39901 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    39961 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    40021 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    40081 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    40141 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    40201 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    40261 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    40321 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    40381 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    40441 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    40501 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    40561 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    40621 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagga a
//
LOCUS       DAYYNQ010000012        39588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000012 DAYYNQ010000000
VERSION     DAYYNQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.012"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..566)
                     /locus_tag="ACHBJ1_000605"
     CDS             complement(21..566)
                     /locus_tag="ACHBJ1_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9429575.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(578..1270)
                     /locus_tag="ACHBJ1_000606"
     CDS             complement(578..1270)
                     /locus_tag="ACHBJ1_000606"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM9429576.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1289..1573)
                     /gene="macP"
                     /locus_tag="ACHBJ1_000607"
     CDS             complement(1289..1573)
                     /gene="macP"
                     /locus_tag="ACHBJ1_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM9429577.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1586..2149)
                     /locus_tag="ACHBJ1_000608"
     CDS             complement(1586..2149)
                     /locus_tag="ACHBJ1_000608"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM9429578.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2377..3039
                     /locus_tag="ACHBJ1_000609"
     CDS             2377..3039
                     /locus_tag="ACHBJ1_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM9429579.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3163..4359
                     /locus_tag="ACHBJ1_000610"
     CDS             3163..4359
                     /locus_tag="ACHBJ1_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM9429580.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4460..5836)
                     /locus_tag="ACHBJ1_000611"
     CDS             complement(4460..5836)
                     /locus_tag="ACHBJ1_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9429581.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5873..6740)
                     /locus_tag="ACHBJ1_000612"
                     /pseudo
     CDS             complement(5873..6740)
                     /locus_tag="ACHBJ1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6829..7854)
                     /locus_tag="ACHBJ1_000613"
     CDS             complement(6829..7854)
                     /locus_tag="ACHBJ1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9429582.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7872..8546)
                     /locus_tag="ACHBJ1_000614"
     CDS             complement(7872..8546)
                     /locus_tag="ACHBJ1_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9429583.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8730..9623)
                     /locus_tag="ACHBJ1_000615"
     CDS             complement(8730..9623)
                     /locus_tag="ACHBJ1_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9429584.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9697..11394)
                     /locus_tag="ACHBJ1_000616"
     CDS             complement(9697..11394)
                     /locus_tag="ACHBJ1_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9429585.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11387..12700)
                     /locus_tag="ACHBJ1_000617"
     CDS             complement(11387..12700)
                     /locus_tag="ACHBJ1_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM9429586.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12700..16641)
                     /locus_tag="ACHBJ1_000618"
     CDS             complement(12700..16641)
                     /locus_tag="ACHBJ1_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9429587.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16750..17208)
                     /locus_tag="ACHBJ1_000619"
     CDS             complement(16750..17208)
                     /locus_tag="ACHBJ1_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9429588.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17506..18024)
                     /locus_tag="ACHBJ1_000620"
     CDS             complement(17506..18024)
                     /locus_tag="ACHBJ1_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9429589.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18026..18643)
                     /locus_tag="ACHBJ1_000621"
     CDS             complement(18026..18643)
                     /locus_tag="ACHBJ1_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM9429590.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18640..19494)
                     /locus_tag="ACHBJ1_000622"
     CDS             complement(18640..19494)
                     /locus_tag="ACHBJ1_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429591.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19491..20402)
                     /locus_tag="ACHBJ1_000623"
     CDS             complement(19491..20402)
                     /locus_tag="ACHBJ1_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429592.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20443..21777)
                     /locus_tag="ACHBJ1_000624"
     CDS             complement(20443..21777)
                     /locus_tag="ACHBJ1_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9429593.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            21985..22815
                     /locus_tag="ACHBJ1_000625"
     CDS             21985..22815
                     /locus_tag="ACHBJ1_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM9429594.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23059..23308)
                     /locus_tag="ACHBJ1_000626"
                     /pseudo
     CDS             complement(23059..23308)
                     /locus_tag="ACHBJ1_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23499..23693)
                     /locus_tag="ACHBJ1_000627"
     CDS             complement(23499..23693)
                     /locus_tag="ACHBJ1_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429595.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24198..24383)
                     /locus_tag="ACHBJ1_000628"
     CDS             complement(24198..24383)
                     /locus_tag="ACHBJ1_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429596.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25233..25574)
                     /locus_tag="ACHBJ1_000629"
     CDS             complement(25233..25574)
                     /locus_tag="ACHBJ1_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9429597.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25574..25804)
                     /locus_tag="ACHBJ1_000630"
     CDS             complement(25574..25804)
                     /locus_tag="ACHBJ1_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9429598.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26091..26429
                     /locus_tag="ACHBJ1_000631"
     CDS             26091..26429
                     /locus_tag="ACHBJ1_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429599.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26634..27196)
                     /locus_tag="ACHBJ1_000632"
                     /pseudo
     CDS             complement(26634..27196)
                     /locus_tag="ACHBJ1_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27928..28509)
                     /locus_tag="ACHBJ1_000633"
     CDS             complement(27928..28509)
                     /locus_tag="ACHBJ1_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9429600.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28692..29318)
                     /locus_tag="ACHBJ1_000634"
     CDS             complement(28692..29318)
                     /locus_tag="ACHBJ1_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9429601.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29340..30671)
                     /locus_tag="ACHBJ1_000635"
     CDS             complement(29340..30671)
                     /locus_tag="ACHBJ1_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM9429602.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30826..31227)
                     /locus_tag="ACHBJ1_000636"
     CDS             complement(30826..31227)
                     /locus_tag="ACHBJ1_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM9429603.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31313..31519)
                     /locus_tag="ACHBJ1_000637"
     CDS             complement(31313..31519)
                     /locus_tag="ACHBJ1_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429604.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31614..33116)
                     /locus_tag="ACHBJ1_000638"
     CDS             complement(31614..33116)
                     /locus_tag="ACHBJ1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM9429605.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33134..33589)
                     /gene="argR"
                     /locus_tag="ACHBJ1_000639"
     CDS             complement(33134..33589)
                     /gene="argR"
                     /locus_tag="ACHBJ1_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9429606.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33687..34304)
                     /locus_tag="ACHBJ1_000640"
     CDS             complement(33687..34304)
                     /locus_tag="ACHBJ1_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM9429607.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34525..35898
                     /locus_tag="ACHBJ1_000641"
     CDS             34525..35898
                     /locus_tag="ACHBJ1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429608.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            35973..37085
                     /locus_tag="ACHBJ1_000642"
     CDS             35973..37085
                     /locus_tag="ACHBJ1_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9429609.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37157..38794)
                     /locus_tag="ACHBJ1_000643"
     CDS             complement(37157..38794)
                     /locus_tag="ACHBJ1_000643"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM9429610.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(38962..39570)
                     /locus_tag="ACHBJ1_000644"
     CDS             complement(38962..39570)
                     /locus_tag="ACHBJ1_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9429611.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        11367 a         8428 c         6883 g        12910 t
ORIGIN      
        1 tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc
       61 aatgcccatt catgtccaac gggatcgaaa cttgccacgg aatctttgaa aatatggagt
      121 ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg
      181 ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca
      241 ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct
      301 tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc
      361 ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca
      421 aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca
      481 attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg
      541 tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt
      601 cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg
      661 tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact
      721 gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat
      781 tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca
      841 atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat
      901 atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc
      961 ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta
     1021 agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt
     1081 ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga
     1141 tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata
     1201 atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac
     1261 cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac
     1321 ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct
     1381 tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat
     1441 aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt
     1501 ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg
     1561 gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa
     1621 tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt
     1681 caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga
     1741 ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa
     1801 gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt
     1861 ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa
     1921 tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt
     1981 gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag
     2041 ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga
     2101 tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg
     2161 ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta
     2221 ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc
     2281 gatactcttt ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat
     2341 tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg
     2401 cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta
     2461 ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa
     2521 aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa
     2581 agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat
     2641 gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc
     2701 cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac
     2761 cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca
     2821 aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta
     2881 gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa
     2941 ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta
     3001 aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag
     3061 actcggaaca ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt
     3121 catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact
     3181 gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt
     3241 tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag
     3301 caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa
     3361 cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct
     3421 gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat
     3481 attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct
     3541 gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa
     3601 tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca
     3661 gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt
     3721 taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg
     3781 aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact
     3841 ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat
     3901 aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc
     3961 cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca
     4021 gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat
     4081 gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca
     4141 gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat
     4201 atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt
     4261 atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa
     4321 gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta
     4381 aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt
     4441 ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa
     4501 aataaatgat cagtccaata accattacga taccgaacac gatccatgtc acagtttgca
     4561 gacgaagcat caatacaata caagtcaaaa tggagacgat tggcaatagc ggaacgaatg
     4621 gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta
     4681 gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca
     4741 atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg
     4801 gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga
     4861 cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta
     4921 aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa
     4981 aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg
     5041 tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat
     5101 ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag
     5161 caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct
     5221 gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa
     5281 atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga
     5341 tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg
     5401 gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt
     5461 gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca
     5521 acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg
     5581 gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga
     5641 caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg
     5701 caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca
     5761 aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg atctctttct
     5821 tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg
     5881 ttttcatata ccctaaaagg gataccacct aaaatgacta aaaggcaacc aacaataaca
     5941 taaatgagct cttttttcgt ttttctttcg ccaaggatga aaatcccacc taaagtagag
     6001 ataatgatcc ccatttgtga aagtgagaag ccaacagcta aaccaagtga tttgactgtt
     6061 agaagcatgc agatgtttcc taagccccat aataatccac agatcatgtt tttccacaca
     6121 aactgatcga ctttaacttt acggaaagca aagaagcttg cgcctaagat cattccgata
     6181 ctttgaggta agataattga catcgcatcc aaattcgccc agttgacgat gatggtatat
     6241 acaccataac caatcgtaga gaagatcaat gctctaaaac ccttaccaaa atcaagcatt
     6301 ttattatctg tatctatttt tccttcgtca tcttgtcggg cagtcaaata cgcaccgctt
     6361 acaagaagag ctaaagcaat aataccgtat acataatctt tcgtttgtgt ccattcatgg
     6421 aagaaaacgg ctcccgcaat cgtattcaaa attaattgaa aaccagtaga caatggtagc
     6481 cctacagaaa cacccatgta cttcattcca tggaactgtc cattttgtcc aacactccaa
     6541 gctaagccgg ataaaaatcc gatcaagaaa atccaagggg tgattacagg actaacaacg
     6601 aaaaagacaa ctaaagaaaa cagaaatgca ccgatcgtca ttcctagtgt ttgttgattg
     6661 gcatcgccac caattttacc acttactaaa ccaatactac cccatgccaa catagggact
     6721 aatgctatta aaatttccat aaccaatctc cttttatttc acatgataag aaacttatca
     6781 gcgaaaacgt taacaacaca agcacttttt ttcgtttcta acgattttct aacgtttttc
     6841 attataaaaa tttaagattg ggaaaaataa agtcattgtt gtcccttttc cttcagttga
     6901 ttcagccgtc aaatggatac ctaatttttc ggccagattt tttgctaaat aaagtcctaa
     6961 tcctgttgaa tgtgttttgc taagtcgacc atttctccca gtaaatccct tatcaaagat
     7021 ccgtttcaag tcttccgctg ggataccgat ccctgaatct ttaactgaaa ggctgactcc
     7081 ttgcgcttcc ttttctatgc tcacaatgat gtttccgtga tccggcgtat atttgatggc
     7141 attactaagc aattggttga agataaaagc gatccattta gcatcggtca aaacagtatg
     7201 atcctcacca acgatctcgt agcgaatatt tttttggata aaataatttg cctgactgcg
     7261 tatcacaggc tgaatgattc cttttagtcc atattcttgg atgaggtagt cccgcgagaa
     7321 actgtcgagt cttgcataat acaaaacttg ttctacgtat tcatcgattt ttgataattc
     7381 attttctagt aatgtatact tttgatcgtc aatatcgaac tcgatcgaac gaactagcaa
     7441 tttagaagcg gaaagtggca ctttgatttc atggacccat gagtcaatat aatctttttg
     7501 gtcttgttga ttactgattg ctgattgcat tacttcttgg tgttcacgga tcagctgatt
     7561 gatatattct tctaccagtt tttcttcttc tgtcctccca ccactcaaat aattttgcaa
     7621 aggggaagtt ttttgtatgt cggctaattt tccccaccat cttctcttgc tcaagtaacc
     7681 acccagcaga taaaaaatca gaaagacgcc ctcaatcaaa gctaaataag caattgtgct
     7741 caaatcaact ttgatgtttg gcgccagcca catcacaaag atcgtcaatg caataaaaaa
     7801 cagccatccc aagatcaatg tccactgatc tttcaaatat ttaaaaaaat tcatcttaac
     7861 cgctcatcct ttcagggaac gatatatccc tgtcctactt ttgtttcgat atatccttgg
     7921 atacctgcct gttcgatttt tcggcgtaac cgattgatgt tgactgtcaa tgtattgtca
     7981 tctacaaatc gttcgtcatc ccacagtgct cgcagcaatt tttcccgact taagatcttt
     8041 ccattgtgct tcatcaaaat aaataataga cgatattcat ttttgctcaa atcaatgatc
     8101 tctccgttga tttccatact tccgttgtca acattcaacg taatgccatt gtggctcatc
     8161 atttcactag ataattctgt gtagttgtaa gaacggcgaa gtaatgcatt gatttttgca
     8221 atcagtacat cgatttggaa atgttttgtc acaaaatcat ctgcccccat gttcatagcc
     8281 atgatcatat ccatattcgt attcctgctg gagataaaaa tgatcggtac tttcgatact
     8341 tcacgtattt tttgacacca ataatagcca tccaaaacgg gaagattaat atctagtaat
     8401 acaagctgtg gttcttctct ttcaaagtct gagaacactt gattgaaatc ggtcgttcca
     8461 aaggtttcaa attgccattt ctcaagttcc tccatgatca attgtcgaat gatctcttcg
     8521 tcttcgacta ccatgatttt tgccataaaa aatccctcca tctttcttct tgctactaca
     8581 atatcaaaga agcccttgtg aaacaagttg gcttacctgg aaaatgtcat tttagcaaac
     8641 gttaaaaaaa actggaaata tgcgatttaa tcgcgtttgt ctcttccaag cagcttaaat
     8701 ttattatgtt attcttaata attggtctat tatctgttat ttttctttcg ttgtttttgt
     8761 cttttaaatt ttcgataaca ccaataaccg aaaagcgtta gcactagcaa tatacctaga
     8821 aggagtataa gtaatcgata aagatggtat ttttttgtct gttctatctt tgaagagact
     8881 tcttctgtaa agggaactcg tcttcctgtc actagtaatc ggtgagtgtt cacggtgtaa
     8941 ggtgtacaag tgataagtgt gactagatcc tgcttttctc taattgctaa tgtatttgtt
     9001 tttgttggaa ggacaacaat tttctcaacg acttcataag cgagcatccg accatacact
     9061 tcaagataga atcggtcatc ttgctccatt tcttcaagat ccgtaaagag ttttttctca
     9121 gcaataccgc tatgggcggt aatcacggaa tgtgtactct ctcctccagt tggaaaagag
     9181 gtgccttgca ggactgtcgc tcctctatct aacaaaagat cattcgtttc atcaaataaa
     9241 ggcaagctaa cattgatttt cggaatatag atggctccga tcatatgttg ttcgtaatat
     9301 tcttttcctg gattttggac atttctaacc gcttgctcaa aaggatcttt gacttgtccc
     9361 attcctggga ttactgatgt atgagcaagc gtctgatttt tcttttcatg cactgctaat
     9421 tgttgctcat gtttttcttc tttatcgcgt tcaagctgct tttgataatt ctcgatcctt
     9481 ctttgatcca aataattgct gagagcatct gctaaaaatg gatatgtaaa aactgcagct
     9541 ccggcaagaa acatcactgc cataaataat tgtaataaaa gactgatttg ggtttgaggt
     9601 ctattaccta ttttcttttt cattttcttc accttcttat ttaaaaaaac tgcagcaacc
     9661 tttataaaac cgattgctgc agcttatttt tgttgcttat acttcagact tcattttaga
     9721 tttacggtac cagctgtacg ctccaatcat caaacttatt ccgattagta agaatgccaa
     9781 gagcccgttc ccaccagtag caggaagtga cccgcgtttc atattcggta ctctttctgg
     9841 ccctggattt tctactccta cgatctgaat ttcttcatta tagcttccat aagagatgat
     9901 aaattcagta aaggctgcct cacctgttgg aagtacataa ccttctggcg ccatggtctc
     9961 tttcatttga taggttccat aatctaaccc ttgaacctca aagtatccat ttccatctgc
    10021 gacaaattct gttgcttgtc cgcttgttgc ccactcaatt ctatctgctc ctgattcata
    10081 aacagctaaa ggcgactccc ctttataaaa tattgcgtat tcagaacggt cttcattcca
    10141 caattcgaat cttgcatctt taagcaattc actgcttgat gcatcatatt tttcaaactt
    10201 tcttccacct gttgtgactt tctctggcgg ctctactcct tcttcataat caaattcatc
    10261 atttcgtccg atttcaataa ccgctgtgtt gttatggaaa tcatccgctt ctagttctgt
    10321 ggaaaccttc aaattatacg tgatcgttaa atctcctcca cgtgcatctg ctaatctacc
    10381 taaagtatct actttggcta ctccattttc ttctgtcaaa atcgtcacga caaacccgtt
    10441 gcttactact tctactatat aatcttgtcc ttttgttaaa atcgtctcgc cggctcgcac
    10501 ttctatttcc tgatcataat aagttagacc aactgtaggc gtatctgtga ttttgtaatt
    10561 tttgataaca gcgcttccgt tttcaaggac agaatcgatg aaataaggaa taggtgctgt
    10621 gatcgtgtaa gaagccgttt ttcctctttc tagatcaata taagaaatcg tttcttgttc
    10681 tccagcttca ttaataatcg ttacttgtct aaggtcgctt tcctctacat tcatctgttt
    10741 atctgcttct ctgatttcgt ttttcggata caaatgcaca tctgtattgt atgtgtcttt
    10801 ccatgtagaa ccatcccaca cgccatccac catatcaggc atcatcactg gcatcataac
    10861 aaccattgga gacgcgattc tggaaatatg agctggcgaa taagtttcta agaaaagata
    10921 gactttatct ctagaagaag gtgcttcttc cgtcacttgg acggtagaga ataccgcttc
    10981 tccagcttca ttcgttgttt gtttgtcaat acttgtcagt cgatatttta aaaaccaact
    11041 ttctgtgtgc cgtttttgaa tccattcaat cgcttcggca gaattaagac cgtcttctcg
    11101 ttccggtgtt aaaggattat ctcgaatcaa atcgtaatat acttccgtta cgtcaaacaa
    11161 atcaaaatcg actcctggaa tattttcttc accaaaatcc gtagaaacca atccgctatt
    11221 cggtcgttct tccggaacag atgagaaacc ttttttgtgt aatgtaatcg tcacttcact
    11281 tggtttttgt gcgacttctt cggctttgac tagttttccg ttcccgaaaa tccctattcc
    11341 cgcagtaacg agtaacagta cactcattcc cgtgacaatc atttttttat tcattttttt
    11401 ctccccgttc tttttttatt aataaaacag cacctaacaa taaaagaatt cctagtaaag
    11461 aaatcaacgt tcttgcttca ttcgtttgcg ggaatacgga ccattttcct gattgacctg
    11521 gattgcttgg accactcggc tctttcggtt tgtccgttgt ttcttttacg gtctcgttat
    11581 aaacacgtgg tgttgcggat tcataggcag agactggaac tttcccggat tctaattcag
    11641 ccatctcctg tccgttaact actacatatt gtggttggcc ttttgcatcc acccaaaatt
    11701 cagggatgat gacctcgatt ctcttacttg cttcatccat ctcgtaacct tccacacctt
    11761 ttaattcttc aaaataatag gtgccagaag gcaagaaacg ttgtcctgtc gtaacaaggc
    11821 cctctttatc cgaaatgaat ttgcttacat ttttgtcatt caaaggatca ttgtctgctg
    11881 gtttgatcca ttgatttttc aagtcattag caggagccat atctaaataa tatttccccc
    11941 cttctatcat ctgatataga gcaaaaacag caccttctaa tggttttcct ttttcagttg
    12001 ttcctctttg acgtccatac ttgaagaagt atggatctct tagataaccg acatttttag
    12061 gatagatatg aatctcttgc agttcctcgg ttgattctgt cgggtgataa attggcaaaa
    12121 tagcagcaat tgggatagct gtctgatcga tatcaacatc caatccaact tctggatcaa
    12181 tcgattcttc gaaaatcaaa taaactgcat cccgatcttg gtttttctta ggtgctttga
    12241 tacgtgcaat cccgtctcct aatccgccaa ccgtatcttt cccagttgtt accgaagtcc
    12301 ctatttggac aagcctttct tctgtgatta gctgacggat attgtttctg ttcgtttcag
    12361 cgatttgctg gacgaattct ttaggagtca tgttttcagc agcttttttt tcatgataat
    12421 aatcggtcat gtcgtaaatg gcaaatgtcg ctccatttag tggcaccgtc tgtgaaatga
    12481 tatctgctgt ttcccctgct tcctcagaga tagccaagcc atcattttca tggtattcaa
    12541 attgatcgtt ataatcgata tctcgaatca tccgtttatg caacacgaat tccacgacat
    12601 cttcccctga ttcttctgcc gacatcgcat gtggaagaag gactgctgca agcggtagta
    12661 aaacaagtaa ttgaagcatc aaacacaaca gctttttcat taccttgctc cctccttttt
    12721 agattgtctg acaaacaaac cataggctgc tcctgcaagc gccatgaaga atgcagcaag
    12781 caggatatag aataatcgtc cggatcctcc tgtagcaggc aacggatttt caggatcgtt
    12841 cccgacacta agctgtaatc tgattcgatt tgtctccaaa agttcatatt ctaactcttg
    12901 ctgtagatca ggcgtctctg cttcttcgtt tggataatac gaaatctcaa cttttagtgt
    12961 acccgcttct tttcctggaa ctacccgatc attttctatg tcaggttctt ctcgataact
    13021 aatttctaaa ataaagtaac ctggaagttt tctatagctg tcaggtgact tcacttcctt
    13081 gatccgatag accccttcca acaagtctga agcattgaat agggaagaat cgtttggatc
    13141 tggtgccaag cctccctcga tgatttcata ctctcctgca ctattttgtt tctcaattga
    13201 aaattctgcg ccttcaagac gtcggctatt catactgtca attttcaaaa gttccaaatc
    13261 gattggcgct agttcatctt ctaaggttac ttgtagtaca ccatcgatga tttcatagcc
    13321 ttccggcaag ggagcagttt cgtcttcgat gaccgtatct tcaatgataa acttaccttc
    13381 ttcattcagt gcaaactgga acacttgatc agatagccga tacccatttg gtgcgacaga
    13441 ttccgagacc tcatattcac caatagacag atgcagaggt tggtcgtaat ttaaatcgac
    13501 atacttttct actcctacgg cacgagaata gaagtatccg atcccaggca aacgtttcga
    13561 ttcttctggt tctaacggat taccagtgta tgctcgatac agcgggaagg aagaatcccc
    13621 cactttcctg atatcgaaac ctgcaccatc taatgtatgg ccttcatggt ctaattttct
    13681 aactcggatc tcccgataat caaacgtatt gtaagcagtg aatgctaccc ataaacgatc
    13741 ctcttcatct gttgtatacg tgagatattg ccaattttct tcacctgttt gcttgagttg
    13801 gatagtccct atagcattat cgtaaagtcg caagatccat gtgtcatttt catcaggttc
    13861 tttgaaacct tctgggttct ggctctcaat gattttgtat tctgcatagt tggtagtttc
    13921 ttcactatcc ttataaacaa atgaatctgg ctctccgatt ggatgaataa ctttctcacc
    13981 atccacgatt acatatttcc cgtcttcatc tactgaatag aacgtgagtc tccctttctc
    14041 ttcctctact tcgttcacac cagaaagccc tttcgctact ggatatccga tttccagtcc
    14101 gttaccatct gtttcataaa gttcaaactc tgcgccattg attggcatat tactgccttc
    14161 aattagttta ttgatattca tgtggattgg cttgaattca tttcgcattt ccagtttgac
    14221 atacaactca tcatactctg tgccttctat gttaggctct tgtacaacag aaagcttgtg
    14281 atattctcct tcataatagg aattcggatc tttaccttcc ggtccgccga ctctcgaacc
    14341 agatgtatat tcatctacaa gctcgtagtg acctaacatc tcatcttcgt tggctgcata
    14401 ttttttgatt tggaattgat acgtctcatc tactaactga taacgtggtg gtccagcgat
    14461 ctctctgatc caaaatatgt cgctgactgc tccagtttca tctcctaaaa tcggcatctt
    14521 cgtttcaaag atcgctattc cgtccgtggt agttttactt gtttcgattg gttcggcttc
    14581 cccactctct ggattttggt catataatgc aaattcgact ccagccaacg gctcgccatt
    14641 ttgacccttc ttgctgactt ggaaattacg gcctttatgt tcgtaacgaa tggaagaaga
    14701 tgggaaatac atccgtccag tagtgatatc attctcataa tcctccaggt tctgaatata
    14761 ggccatattg ttcactgtgc aaaaatttcc gtcttgagct cccgcatcaa gttttacttg
    14821 gtacttgatc ctgaccattt ccccttcacc taaagaaatc ggagaaaatt tataagcttg
    14881 gttttcccgt ccgacatacg ttgtcggagt cgccactact tctggtgggg cttgtccatg
    14941 acgttcttca tcccatattt ctggctcttg gtacttcatg ccgttttctc ctggatctag
    15001 atggtaaaac tgaatatctg ccgatctgcc agctccaaat ggtgagacat taaaaccagt
    15061 ttcatcataa agtgtggctt gctggatcgt tctcttgaag gtagaagaga tatcctccaa
    15121 ggctgtaaca atgtcattct tgttgttcgc attgtaatat cgcggtgtta cgccatcttc
    15181 ttctaaatca gcaatatttt tcaatacatt tcgtccaata gcatctagtc gttgtctccc
    15241 gctaacgttt ccatcgattc ctaaaccaat ggagaagata cttggcgggt tctcttgata
    15301 cttgtttttc agccaatgat gataagcaac tgtgttgatc gcagatgact tttcttgcga
    15361 agtaggactg ccattcgtat tgttattaga agaaccatca ccaacccagt gagtcgcttt
    15421 attatctcct tcttttacct ctccataacg atagtgtaca tttacttcat cttctggacg
    15481 gttaaagtca gatgcatagg taactggatg agtaaaccct gcattagaag tattcccaga
    15541 aaactcctca aaatttctga acaaaacgtc attatcttca gcaatcttat cttccatcac
    15601 agtccagttg ctccaagctc cattagtgct cgtacgataa cttgactgaa tcggtgcata
    15661 actgaaggtc ggtgtcccat ctcccacaac gattaggatt ttttctggat ttctattttc
    15721 tccgccattg tctttataca atgtttcata gccattttta agccctagtg taagcggagt
    15781 tcctccgatt ggtgtacgat ttaatgctgt attctcactt cccgtcaaac gattgatatc
    15841 atttgtcaat ggaaagatgt ccgaactgat ttgctcatgg ttattgatag ctgtactatt
    15901 atggtaaaaa ttcaccatac cgattcgaat atccagactc gggtcagaga gcagatcttt
    15961 actcatttcg tttactgcct caatcaacgc agcattttta gtttgactat tggtcccagc
    16021 ggttaattca ctcatactcg ctgacttatc tagaacaaaa acgatatcga ttggactgat
    16081 ttcctttttc tcgcccccga tgacatctag atagacattg taaagggttg ttgcatcttc
    16141 aggatgtaga tcagatgctg tgctgcttgg cagaacaggt tctacccatt ttttgatgta
    16201 cgccccgtta taatccaagt aactgttttc aaaattccgt tcatttcccc aaagctgaac
    16261 ggtttgagct ggaacgtttc tatcttcttc gcctgcagtt gatccattat tagtagcata
    16321 gtcgtaattt cgccagttcg ctcccccgtt tgtataatca ctatcatcga tcaagttctc
    16381 tccgtcatat ggcgagcctt tgtctccttg atagcgttca ttgctttgtg tccaatttcc
    16441 taacactttt gtatagtcat tcgttgtaaa cttcgggtag cgatgctgtt cgttttcaac
    16501 ttctccgcta ggatagatat attcataggg gtcttgcgta gctgttggcc caagacgctc
    16561 caaaataccg ttgaataact ggcgttgaat gcgctggagt gcttgaccgc tgggtacgat
    16621 tggttcgtcc agcttttcca tctcattttc ttcgctacgt tgaatttcag cggaatcttc
    16681 tgtatcagtt tcattttctt gtaattcttg gttatctatt ctttcatcaa ccaccacttg
    16741 aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc
    16801 ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc
    16861 gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt
    16921 tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc
    16981 actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt
    17041 cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga
    17101 ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag
    17161 caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc
    17221 ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata
    17281 atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt
    17341 atacaacatt tattattaaa attaaattat aaaattttat aacgttctca tttaacaaaa
    17401 cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag
    17461 cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga taacggcagc
    17521 gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag taggataatg
    17581 taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg ctaatattcc
    17641 tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg ctgtatgtcc
    17701 tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac gatttttttc
    17761 ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa aaaaatagat
    17821 caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca agactaaaac
    17881 agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa atttcaaaaa
    17941 attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa tactatcatc
    18001 gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt tcttgcgtac
    18061 gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat aaagcaaaat
    18121 agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa
    18181 gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt
    18241 gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga agcaaaaaca
    18301 acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa atctgataat
    18361 cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca taaagagaga
    18421 agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca ttttttttgt
    18481 aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa acaggcaaga
    18541 cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca atgatttcta
    18601 agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg aacctcctgt
    18661 caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat
    18721 caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt ggttctgaaa
    18781 aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata acagaggacc
    18841 taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca aggcaggttt
    18901 cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt cgatttcagc
    18961 agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa atatattgaa
    19021 cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc agccgatctt
    19081 ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca tcgtagaaag
    19141 aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat
    19201 aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata atgtatttct
    19261 tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc atacgatttt
    19321 ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca atgaagtaaa
    19381 caccatccaa agcaatggaa tgatcaaaat gattcctatt actgtcatga ggacataact
    19441 tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca tcatttgccg
    19501 tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa cgtcagaacc
    19561 aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc aaaagctacg
    19621 aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg tccgcctgtt
    19681 ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat cgtagaggtg
    19741 atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt gaactttccc
    19801 caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac attttgtaat
    19861 gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa aatcaacgca
    19921 ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa ccagcccaga
    19981 aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat gtagactgct
    20041 actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat tcctttgatt
    20101 ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag
    20161 atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg aacgaacatt
    20221 cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata atcggtgaaa
    20281 ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc gataataaaa
    20341 ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga taaacgcttc
    20401 aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat tttgcttgac
    20461 taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt ttgttcctaa
    20521 aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac
    20581 tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca tttttccttt
    20641 atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg ccatgatatc
    20701 aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt tgatgaattc
    20761 ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc cgccacccca
    20821 cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa aatcaaaatc
    20881 atccggtgca ttttccatca aactagagta ataattgata ttttgggcac gcatggctac
    20941 taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta atttattgat
    21001 cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt ccactttatt
    21061 tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt
    21121 tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc gtgtaaattc
    21181 ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc aagttgtcgg
    21241 tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa aaatgacttg
    21301 tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg tatcccaaag
    21361 ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat tagttgcttg
    21421 ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat cgggtgcatt
    21481 tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct gatacttata
    21541 atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt cgatagcttc
    21601 ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc catcacttgt
    21661 tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg atccgcatcc
    21721 tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt ttttcatatt
    21781 ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc attttgttct
    21841 gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc aatactttat
    21901 ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg agaatgtatt
    21961 aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca gtttgaagca
    22021 aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat cggcggccaa
    22081 tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg ccgaggagaa
    22141 gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca cgtggaaatg
    22201 ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca agaagcagcg
    22261 atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca gcacgcgatc
    22321 gtagatggct taggacctcg tcctgctgac agcgtgggca atccatggag cggtgcctat
    22381 atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc cgaatcacaa
    22441 ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt aaaagatatg
    22501 ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg tgcagtgaaa
    22561 gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg tgacaaagaa
    22621 cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag tagtgaaggt
    22681 tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt gaaagaacct
    22741 aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta tgggacacct
    22801 ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca cacgctttgt
    22861 gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg ttttctctag
    22921 agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata ttttatgttc
    22981 aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta ctactcctta
    23041 gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac tatcatgttt
    23101 ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt ttgtcacata
    23161 gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg cacaatctct
    23221 tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt ctatactttg
    23281 gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta tttcccttta
    23341 ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc tcaattaggt
    23401 ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg gttttcagaa
    23461 aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga ttttttataa
    23521 atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga acctatgaaa
    23581 tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa tatatttatc
    23641 agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt catagactct
    23701 cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa attaaagggg
    23761 cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct tgtgtttaat
    23821 aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg tgacctccaa
    23881 tttttaactc ttctatctcc catccagtat acataacctt gatacttcga ccacttatat
    23941 atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt gctacttgca
    24001 tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa ctattaattt
    24061 ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca ctttctgtct
    24121 catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac gctggaatag
    24181 ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa aaatacaatg
    24241 aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag caactcatta
    24301 ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa gatggttagg
    24361 aatcctttag aatttttact cattcataga cacttcctta aataactttt attatagaag
    24421 gtattgtaaa taataatatt gacaaaaagt gactcaaaat aaaaaaaacc ttacattata
    24481 ctggaatgaa ctgtattgat agtgtaccta aaagctctac tgacataaga tcgtgtaatc
    24541 ggtaaagtaa ttttattaat tatacgggat gtaatcaatt agagaataaa ctaatcataa
    24601 aaggtacttt tacattcata attgtcgctt tgagattcta ctttggttca ctatctgacc
    24661 caattgcaca ttttcgatca ttacaagtat tggtgtcgtt agaacaacta aataaaaact
    24721 agcggtagat ttttcagaat ctatgctaat ttctgtatgg atgttccagt gatggcaaat
    24781 ttctcttttc attgtttttc ttgtcgcatg atatgccttt ttgtgttatt cagttaaact
    24841 atagaaattc tttcctagac agatcttgat cccaaatttt acgtttttga gcatcgatat
    24901 agtttaaaat gacattactc atatctaaag agtttagatt ttttaggact aaaactgtca
    24961 gttgttcttt ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata cgttaagata
    25021 tgtcgtgaaa aaacaagttt tgtgacacaa aaaaacgaat gacttgagga aatcacctca
    25081 atcattcgtt taaaatgcta tatattcttt agtttaagat acggaaatta atgccatcga
    25141 ccacactagt agaatgaacg acatttactt ggcctttttt taatttcgtt atgtttagca
    25201 tataaaaata ttatagagtt tacttacaat gcttaaaaca tatactgtat aatctgatca
    25261 attttagcca tgtcttgcaa tggtaagttt tctatttttt tgagttttcg ttccttaaaa
    25321 tcaagagatt ttaattgtgt aataattact tgtcctttta tatctagctc ttcaggtaat
    25381 gagtaacgtg tcggtaaatt ctttatagtg gaagtaattg gacatatgac cgaaaacatt
    25441 gttttacggt taaattcata acgagagacg actagtcctg gtcgtctttt ttgaatttct
    25501 tttcctacgg agggatcaaa atcaatccat acaatatcac cttttttagg aatataaatt
    25561 ctttcttctg acactataaa atttctctcc cttcaggagt taaatcttcc cattcgtctt
    25621 tctcataata ctcggcctct tccccaccac taaatggatc ttcaattttt ggaactaacg
    25681 taattgttcc atcatcagaa tacacaacga tatattcttg gttttctgaa ggtttctttc
    25741 cgttatcaga aggtaaagtt accactacag aacttccttg taatctagat tttgtaacaa
    25801 gcattatcat cactccctta ataaaagtat actatataac actaagtaac gcaaaattcc
    25861 cctttgtaaa ctatctagaa aattgttttg tttaaagtat atcttattgg atataaatag
    25921 aaataaattg tagccagttc attttttctt taactaaaaa aataatccaa gaaacattga
    25981 acctatggat ccaaaagcta cccaaatact atagctaacc ccccgctacc ttcaattttg
    26041 cagataaaac gctttgggag aaaatttcaa agacaacagg ccatcctatc atgcccgatt
    26101 ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga aagttcacga
    26161 aactagcagg tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt caaatagaaa
    26221 cattggttca acgaatttca actagacaag actttgctat tgtagaacgt gagatcgtag
    26281 ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg gataaatggc
    26341 aacctcttat ctataaattc agagaatata caactgccaa ccagtcgtta actttgaatt
    26401 ttagattcca agaattcaaa gagtcataat gataaacatc gttaggtata aataaaaaag
    26461 actcgcagca cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg actgtgaccc
    26521 tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt tttgggatct
    26581 gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt atgttatacc
    26641 ttctccatta ctaattcagg catctgctga gtaatgattt ttcgcgcaaa agaaatatct
    26701 gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc atggattggt
    26761 gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt ttcatcctga
    26821 tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa cataaatttg
    26881 tctttttctt cattccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa
    26941 aggtcttttt ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa
    27001 atccggtaaa tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta
    27061 acgtcacaac aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg
    27121 gttgtttcgt ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa
    27181 aataacgacc acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag
    27241 aagttcgcac caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga
    27301 caaactctcc agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca
    27361 acaaacatga gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc
    27421 ttacgacctc tgggtaaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat
    27481 gcaaaaatgt agaaagctat tttcacaaaa tttttcttaa tactcgaaac taagcgactt
    27541 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    27601 caaacgaaaa atatgagcag ctatttttat ttggctgttc ttattacttg atgcagggca
    27661 gctttttcac aacctctcca ctaaacggtg ttcaatcagc taaccttcgg tattagctca
    27721 aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaag
    27781 cgctgatttc acaacctcta atacacggtg ttccagaagt agcttcttcg aaaaataagc
    27841 cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    27901 gaccacttct gtcacaacct ctaattatta aagaaccttt tgcgaagtta acaatctttc
    27961 tttgatcaat tgaaaattct gcctaagtag tgatagatcc tgtgtgactt tcaacaataa
    28021 gatacttccc tggatttcag caatcaacgc tttggcttga cggtctgctt cttcatcgga
    28081 cagtttttct tcagaaagtg ccaggatatt ttgtttcgta tacttgaccc attgattgac
    28141 taattctagt agcaacagac ggtattcttc atccactgta ctcaactcaa taatcaaatt
    28201 acctacagga cagccatgat aagtgatctg tgtctcatga tcagcgaaaa tccagtccaa
    28261 catttcttct aatgcttctt ctggctgttt tccagcattt aagatgtctt cgaacaattc
    28321 tgtctgccat acgtccatat gcgcatggat caccgctaga caaacttctt tttttgaaga
    28381 aaagtaataa taaaattgac cttttcctgt atcagaagct gctagaatat ctcggattga
    28441 cgtttcatga tagccttttt tataaataag atctttggtc gcttccaaga tcttttcctt
    28501 tgttttcaat gatattctcc tttttcattt ctcatagtcc tatattatac agtaaattca
    28561 tcattttttc ttcttaatcg tttaaaaaag actgtgccaa attatctgac acagccctca
    28621 ctataaaact gttctcatat agctagatgt tcattgattc aagaactgcg aggacaaaag
    28681 caattcctat cttatgatag ttgctctaat acttcctctg ttgtcatcac ttgatctgca
    28741 ataaagccaa agttcgtcaa cgctgcatcc attgcttctt ggccaggagc accaattgca
    28801 tctgtcgcta ccacaacatt gaatccactc tctaccaatt cacgcatatg ggaatcgcta
    28861 cacaagtttc cgttcattcc accaagaatt actgtgtcga ttccattttt tctcaattgg
    28921 agcgccaaat cattgctttc tggtccaaag attttatgag gagagctgat gatcgtattt
    28981 tcatccaaat aaggtttcaa ttcttcgata aaatcggctc ctgaaccttc ttccactgat
    29041 tcatattgac tttttctagc aaacatctgc tgctcaagca tcatatgttc tcctgcaccg
    29101 ccaaattgcc atttatggtc atgtgggtaa aaataatgag gagaaataaa gagtttgtat
    29161 cctttttctt ttgcttgttt taccatttca atcatatttt cgatcgtatt gtatttttct
    29221 aagatatctt ttgtcaaacc aaagccaccg cctgctggtt tcaagaactc gacttgcggg
    29281 tcagtgagaa ctactgctgt atttttatta ttgaacatgg tggattctcc attcgttatt
    29341 tattttaata catcatttag agcttcactc attgtcggat gagaatagat ttgatctctc
    29401 aataacgtat aaggaagttt tccattcata gctagcgaaa tcaagttgat gacttcatga
    29461 gaatcttccg catagatact tgcccctaga atcaagtcag tttcagggtc gactaaaact
    29521 ttgaatacac ctttaggatc ttctaatact tgtgctttag ggacaccgct tgtcatatac
    29581 ttgaataatt tgtaattgac gccttgtgct ttggcttgtt tttctgttaa acctacattg
    29641 gataaagtcg gagtgataaa tacactgtaa ggcacagctt tccggttatt cgttgttcgt
    29701 ttattctcgc ccttcaactg atccataatg attcgataat catctagaga gatataagtg
    29761 aactgcaatc cgcctttgac gtctccaatc gcccaaatgt tgtctgcatc tgtacgaagg
    29821 tattcatcca cagcgattgc tccgcgatct gttagtttaa catctgtatt ttctaatcca
    29881 agtgatgctg tacttggttt tcttccagtt gcagccaaaa cttttgacgc ttggattgtt
    29941 acttcttggc catcttctgt aaaggtgacg gcagctgttg attcttggtc tgctacttga
    30001 tctacagata cacctagatg gaattcgatt cccgcatttg tcatgtcttc taaaatcatt
    30061 tgcgcgatat cttcatcttc acgtggaaga aattcagaat gggcatcaag aacgacaact
    30121 ttagatccgt attcattgaa cattgatgca aattccaagc caatatatcc agccccgatg
    30181 atcactaatt tttccggtaa ttgttcctga tccatcgctt gcgtagaatc aatcaagtag
    30241 cgacttgtat tcaatccttt gattggtaaa atgattggtt cagaaccagt attgatgaaa
    30301 atccgttcgc cttcaacaat cactttttca ccattattcg tcacttcgat ttgatggtta
    30361 gaaagaaaac gtgctgtacc gtccatcact gtcccagttg cctcatcgct gatcatatga
    30421 taatttttgg cgcgaagcat gcccgtcaat ttttctttcc gagcaacagc ctctgtaaag
    30481 tcgattcctc tttctccatt gaaaatcaat gatttagaag gaatacagcc gatgttgata
    30541 catgttccgc catacatacg tgttgacttt tcaacgacta aaaccgattc accttttgaa
    30601 gctaacgcct tcgctaaagt cttaccgccc tttccaaaac cgatcacaat attttgatat
    30661 ttttccatca tttttccctc gcttcttttt tacctaccga ttggtacaaa aatatgataa
    30721 ccgataattg tctattctcc aaatcatctg ctttatttgt caaaaaaaac cagacaaaaa
    30781 gcggtccttt cacgttttta gtctggttcg atttccttta ttcttctaaa caatcgttac
    30841 ttctacatcg atatttcctc tagttgcttt ggagtaaggg cagaattcat gcgcatcttc
    30901 tgcaattttt tgggcttctt cttcgctgat gccatcgata tgcacttcta atactacacc
    30961 gatctgatag ccgccatcat ctaaattata aagagagaca cgtgctttta ctgtggactc
    31021 tgctgtcatt cctgcttctt tgatcatatg ttgtaccgcc ccattgaaac aagaagcata
    31081 acctgctgca aataattgtt ctggatttgt tccttctgca tggattcctg gaggagtgat
    31141 tttcatatgc atcgagccat ttggtgcctc gacttctcca ttgcgcccgc catggttgat
    31201 cattgctgtt tcgtatgctt ttttcattgt actgctcctt tcgattgacc atttgctact
    31261 tggctataat tatagtgtat gacaagtaag gcgctttcta caaaaattct gcttattcga
    31321 ataccagaat gccgtcttct actatttttc caaaaaacaa aaaaatacta ctcgtgaaca
    31381 acagcgtcat atttgttcca aaataaatag caatcttttc tacataatct tttatatgat
    31441 tgattttaag atacatatat accagaaagt tgaccccata aaccaatact gttaaaaaaa
    31501 taaccatatt cgttaccatt ttttcctcct tttgctggtc tccctaccag cactcgattt
    31561 aaccatacca ctttttttgc cgtccaagca actagccttt caaaaagact ggtttattct
    31621 gctgttaatg attcaagatc agaaaaatgt ccgaataatt ttgctaattt tactaacgcc
    31681 aacttgttct ttccgaattc atcattttct atctgtaata ctagtaacgg ctcatgtaag
    31741 cttaaacgga tcaaaaacca gccgtttccg tatggctctt ctagatcata tcgaatacct
    31801 tcttcatttt ccgggtcgaa gttcatccct ttttcttctt ccaacagtcg cttcatttct
    31861 tctaatatct tgtttccttg ttcgcgtggt tcatcgctga acaattttaa tcggatttct
    31921 tgtacttcta cgggctgttt caactctttg atttgttcat ctaatgtgat accttttctt
    31981 tttaattctg gcaaaagcat caggatcttt gcgatgacat atgcaccatc atccagaaaa
    32041 tagttttcct tgaatgcagc gtgtccgctt gtttcgatgg ctagctgtgt atctttccca
    32101 agacggttaa gttctaatgc tttattgatt acatttcgat agccggaaat ataacgaact
    32161 tggatgcctc ctttttcttc aatgaatctt tgtagatgag aagaagtagg ggaattcgtc
    32221 acgattcttg ttcctggatg ctcttttaat acgattgtac ttaatactgc gatcaaatta
    32281 tttcgattga tcactgttcc agaagcactg acaactgcag aacgatccac atctgtatcg
    32341 aaaatgatcc caagatctgc ttcatttgct aaaacagcct gcttgatact ttccatcgct
    32401 tctttattat caggatttgg gatatgatta ggaaatcggc catctggttc caaaaattgg
    32461 gaacctgttg aatccgcacc taagctattc aatacttttt cagcaaagaa tccaccagca
    32521 ccatttccgg catcaacaat gattttccaa ccatttagtg gtttgctttc tagtgttcca
    32581 atcccctttt taatttttgt gactaaatct tgcgcataag gtgtcaaaag gtctgctttt
    32641 tctacgcttc cagtttgttc gctttttatt ttttgctgct gtgataggat aaaagcaata
    32701 tcttcttttt ctgctcctcc ttctgaggta aagaatttta ttccattata ataataggga
    32761 agatggctag cagtcagcat aaccgcagca tcgcacgaga actgttcaaa ttgagtggac
    32821 ataaacatag caggcgtagt agctaattcg aaatcgatga catgtatccc ttgttctttc
    32881 agactttgga tcaacgcctc tttcagtgct ggtccgctta tcctactgtc atgaccaatc
    32941 ccaatcgtcc attttttatt tggatcggtt gatttcttct ttttctttat ccagtttgct
    33001 agtccccagc cgatcttttt taccatgtct gtcgtcaagt ttacttcata tccttcaact
    33061 ggtaatgcga ttcctcgaat atccgatcca ttttgcagcg ctttcatatc tttcatataa
    33121 aagtcctcct catttattcc ctcatttttt ccagcttttc tgcaaatcgc tgtgcttctt
    33181 cttctgagcg gcaaataata atgaatgtat ctacaccggc catcgttccg acaacttcag
    33241 ggtaagccac ttcatctagc caattcgtta ccacatcggc accatttcgt tcagtcaata
    33301 cgataaccat gaactgaaca gactggatac gcaaaacctc gcgtctaatt gctgtacgca
    33361 aacgttcttc atttaaagaa acagatggct ggttaaataa tgtatagcgt actattttgt
    33421 tttcattttg actttttact aacttcattt ctcgaatgtc tcgagaaatc gttgcttgag
    33481 tcacatcggc gcctgcttca tgcagccgcg caagcagctc ttcctgtgtc ccaataggtt
    33541 gttctgtaat gatctggcgg atcagtgctt gtcgctgttt ctttttcata ttagacacct
    33601 ctcaatgttg attatagcaa aagataagta atatttcttc atattttggt attttttact
    33661 tttatttttt gttttcattc aggttgctaa cctttttcta ttttaacacg caaaatcgtc
    33721 ttcatttttt caggtgctga ttttcttgga tcgctgatgt aaatctcttt gtgttcttta
    33781 gaagctcttt catagccttc ttttgcgatg aatgcttcta atttctcaaa cgttgctggt
    33841 tcttcggcat agggcccgac gtgtaagatt tgggcacaaa gaccttcttc catctttttg
    33901 aatactactt gttctttcaa gtcttccgga agtttgctag aacgctgaat ggcttcttgt
    33961 gctatcgctt ctgtaacaaa gtcaggctgt cggatcatca attgataaga aaaatgttct
    34021 tttttcatca ctggttcatg caaatacttt tcttgcagtc cccattgtcc ttccagtgga
    34081 tagacagtat acatctgata atcaggaatc agccaatttt ttttaggcga catgcgtatt
    34141 gcatagctga ctgcatatag acaggctact cttcgtttaa agtcttcctg attcgggtcg
    34201 ccgatccctc tgatgctaaa gaatgatttt ttaggaacct ctagtatttg cgggcttttt
    34261 ttcatactat agctcttgtc tttacgccat tcaatttttt ccatttttct tcctccgcga
    34321 aattccattt agttatttat tatcatacct ctttttgagt gacttggcta atcgtgttga
    34381 cactattttt cagtttgtgc tagaataagt gaagtaaata aatcgtacag ataacacgtt
    34441 tcggcgtgtc gagagagagg cggtttctat ttttataata gaaatcactc tcttttttta
    34501 cgaaattttt tagaggtgat gtaaatgaca aaacaaaaac agctgctttt agtacctgca
    34561 gtaattgcaa cagggatgat gtcttttgcg ggcgtattga tcgagacggc catgaatgta
    34621 actttcccaa ctcttatcag acagtttgat ttaacgactg cacaagtcca atgggtaaca
    34681 acgatttatc ttttgatgat ttcgatcatt gttccgctgt ctacttattt gaatcgcaat
    34741 tttagtctca aaaaactctt tttagcttca aatctgcttt tcttggcagg tataatgatc
    34801 gatttcttct ctcctacctt catggtccta ttgtttggtc gattgctcca aggtgcagcg
    34861 acaggtatcg ctcttccgtt gatgttccat attatcttga catttgcccc cctcaatcga
    34921 cgaggcgcga tgatgggtgt cggtactttg acaacggcca ttgctccagc aattggcccc
    34981 acttatggag gcattatgac ttcacatttt acatggaatc atatcttcct gtttcttgtg
    35041 cccgttttgt ttctttcttt gattatcggc tggtttgcta ttcccgagat ggcagtacaa
    35101 aaaagtggtt atttggattg gataagcgtt ttaggaattt gtcttttgtt tagtggaagt
    35161 ctaacatttt tgagtacgtt gggaacagtt tccagctgga ttgctttaat catcggcggt
    35221 attggctggt tcctttttta ctatcgaagt aaaaaagtgg agcatccact gatccgactg
    35281 tctattcttt caaataaaac attccgcttg tttttgattg gcttcttagt ttatcagttt
    35341 ttattacttg gtgtatcttt tgtcttgccg aatttccttc agatcgttca aggaagtagt
    35401 ccttttgtcg ctggattagc gatgcttccc ggcgcagcgg ttggtgcttt gctttctcct
    35461 ttttccggga aaatgctgga tcaatatggt ccgaagaagc caatttttgc tgggctgatt
    35521 ctttctttga ttggttggct agcattattc ctactcatag gacacgcttc agtgggtgta
    35581 ttgattgctt gtcatgtgtt ttatatgatc ggtatcggat tatcttacag caatatgatg
    35641 accaccggaa tgaatgcact gaatgaagaa ctacaaggag acgggaacgc aatgttcaat
    35701 accttgcagc agttttctgg cgctgtggct acttcattag tagcggtcat tatcaattac
    35761 gtccagcatc atacttccca taactatgaa gtgtctacta cattaggatc aaaagcagca
    35821 ttaggcgtct tgttgctgtt gcttctcgta agctttgctc gattcgccta ttatctgttc
    35881 tttgctaaaa aagcgtagat tccatatacg gcaggtgttg catctgccgt ttttttgtgt
    35941 gaaaatggtt gtttgtaaaa ggaggctttt ttatggaaaa aattgccatt atcggtggcg
    36001 ggatcattgg tatgacccta gccagttact tagatacatc aaaattcaat gttgctttat
    36061 ttgatgaagg gacaggacag gcaactaaag ctagcgctgg gattatctcc ccttggttgt
    36121 cgaaacgcag aaacaaacaa tggtatcgtc tggctaaaga cggcgctgct tttttcccta
    36181 aacttataaa agatttccaa cttgatcaaa cgatttacgc acaatcaggg acacttttgc
    36241 ttcgacctga aaaagattta gctgaattgg ctgttcttgc cgagaaacga aaagaagaag
    36301 cgccagaaat tggaaacatc gaatggcttt cacctgaaca gacatctcaa gcacttccgt
    36361 tattgaaacc agcagtttca ctgcgtatca gtggtggtgg tcgtctggat ggaaaagcat
    36421 atttagaaca tcttggtcag cgtttagcac aaaaacaaat ggagcagatc caggcaaaag
    36481 tcactttgca taagaagagc gattcttggc tcatcaaggg accgtttgga gaatacacag
    36541 ctgatcgtgt aattttgaca cctggcccgg cacttaagcc tttattacaa gaattaggct
    36601 atgctgcaga tatacgtccg caaaaagggc agctcttagc atttcagaca tcttataaga
    36661 tgagtcaaga ctggcctgtt gctatgcttg acggagaagc tgatttcatt cctttcatgg
    36721 atggaaagat ccttttaggt gctacccatg aaaacgatca aggctgggat ttggctccta
    36781 ctagcgaagc ttataaggga ttgactaaga gtacagaaca gttcttgact gatccagatc
    36841 agataaaaaa ctggccatac gagttccgtg taggtacccg tgcttatacg tcagattttt
    36901 ccccattttt cggcgtactg ccagatgatc caagcttact ggctgctagc ggtctaggct
    36961 cttctggctt gaccacaggt ccttatatcg gctatctatt agccaacttc ttaaatactg
    37021 gtcattggcc tacggaagat tatcaaaagg acatctccac ctatattacg aaaaatccat
    37081 catagaaaaa cagctttttc aagtaaggat cccgagcgat attgcgcatg gattacttct
    37141 tgaaaaagct gttattttag ttcaatttct gaatcaaatt ctccaaactt tcttttgctt
    37201 catctggcaa gagatctttt cctatctgag cttcaatgaa tcgtaaacga atatcaagtc
    37261 gttgtttcac cagattttta taggacgtat cctcaaaaga aacaggaaaa tctggtatag
    37321 gtaaatagtg gatgtcttca aatggaccaa acgacttgaa ttctgccgtt tgttgcacga
    37381 ttctttcaac gattgacaat gtcgtttcag gtttgatttt tttatcatcg aaatatccag
    37441 tattcaggac atagcaggct gtttgctgtt tcaaaaacaa ctctcgaaaa gcttgatagt
    37501 cttccctaag aggatagaca cggaaaggat tagcaaatgg ttcaatgact agctcatcta
    37561 gctgtctttt tgtagtgata ttttccgcgt ctgatcgttt tgttgctaat gttgcaccaa
    37621 aagacgctgc taaaactggg tcattgatct tgataattgg cggaagcgaa tcatctttca
    37681 tgatccaaaa aacagcatcg atcttttcgc taagatgatc gactcggtcg ggtgtcgcat
    37741 aacgtgattt gactgtccgc ccatttccgt ttcgtatatc tcctgttact aggacttttt
    37801 ctcctttact tgtacatgtc acgcccacat tctgacaagt aaggaaagaa gaaatggccg
    37861 gatcatcaga cggatagtct tgtgttttat caaagtaaga aggttccaag gcagtagcaa
    37921 aaccattttt tcgagaaata acgaaagcat cgtcatgcaa gatcgaaaca gagtgacctt
    37981 ccgtatgttt tgctaaggta atcgttgatt ttccagaacc ggacaaccca aatacagcca
    38041 tcgtataggt ctggtcatta ctagcatatt gtttcatccc accgtggcag gcaataaagc
    38101 cattgcgatt tcccaatgcc caagcaagcg tcaaagtcgc tttttttaat tctccaaagt
    38161 aacgtaatcc tagcacagcc gctgtattat ggagagcatc aaacaaaatg agtccatttg
    38221 gaaatgtttc atgtgtccat tcgggatcac aaaagatata aagatctccc tcatcaaagg
    38281 aaacagactt ttgataagtc tctaagatct tcgaattcgc tggctgaaaa ttcagcaaat
    38341 aagaatacaa attgttctca aatccttctg gaatcagcaa atgactttgc acagaaaatt
    38401 cttcagatag gccaacgatt acttttgtat ggtaaaactg gcgcttgcga ctttgataca
    38461 cagcttctct taaaattttc tcgtatttgc tttgttcctt gtctaagtcc tcaaagatcc
    38521 gccgagcact tgctgttctt cccacaatgc ttccatcatt gaatactagt gtcctaacat
    38581 cagctggtaa atctagttct tcagcatgtg agattgttaa atctgtgatg atcgttcctg
    38641 gactcccttt tgctagttga taagctgtct tcctatcaaa aacttcatga acattattac
    38701 cgtaaaatgt cgtttctaca atcgatcgaa tactggataa cagtggattt ccacttgtga
    38761 tttcgtttaa ttcgagactc tcttttgtac tcatggctcc tcctcattcc ctaaacaaaa
    38821 aaacgttttc tttattatac attattcgag tttttatttg tactgctgcg ctgtgggaag
    38881 caaacgagtt atttttcaca caaaaaaacc gagaacttga ataaacaagt ttctcggttt
    38941 catcattttc cttttatcgg cttagtacca gccgtttgct aaccagaatg ttttagcagc
    39001 atcccatgaa ccataacgag atgttacata ttgttctgct acacgttctt gattggcagc
    39061 tgaatagtcg ccgtttaagt atgaagcatc taattgataa cggccgatgt aacgtccgtt
    39121 agtagctgtg tatgaaccgc ctgattcttt ttgtgcgatc cattcttttg cagagcttga
    39181 agttggcgct gttgcagcag ctggggcagc aggagctact tcaactgttt caacaggtgc
    39241 aacgtatgta tgttcttgcg ttgctgtttc agttgtagat gctgtatttt ctgttgtttt
    39301 gttttctgtt gttgctgaag aatcatttga agttgggatt gttaattgtt caccaacata
    39361 aatgcggttg atgtttgcga tcgaattttt ttcagcaatt gcatcgatca ttgagttgtc
    39421 tcctgcaaat ttttgtgaga tcttagataa tgaatcacct gattttactg tataaacttc
    39481 atcagcgtga gcatttgtag ttcctacaaa cattgcagta gcaccagcag caaaagttgt
    39541 acctaaaaca attttcttaa atgaattcat attaaactcc tttttttc
//
LOCUS       DAYYNQ010000013        38440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000013 DAYYNQ010000000
VERSION     DAYYNQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.013"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            412..732
                     /locus_tag="ACHBJ1_000645"
     CDS             412..732
                     /locus_tag="ACHBJ1_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM9429612.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            886..1791
                     /locus_tag="ACHBJ1_000646"
     CDS             886..1791
                     /locus_tag="ACHBJ1_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429613.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1788..2561
                     /locus_tag="ACHBJ1_000647"
     CDS             1788..2561
                     /locus_tag="ACHBJ1_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9429614.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2742..4166)
                     /locus_tag="ACHBJ1_000648"
     CDS             complement(2742..4166)
                     /locus_tag="ACHBJ1_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM9429615.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4377..4823
                     /gene="nrdI"
                     /locus_tag="ACHBJ1_000649"
     CDS             4377..4823
                     /gene="nrdI"
                     /locus_tag="ACHBJ1_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM9429616.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4888..6870)
                     /locus_tag="ACHBJ1_000650"
     CDS             complement(4888..6870)
                     /locus_tag="ACHBJ1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM9429617.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6884..7780)
                     /gene="murQ"
                     /locus_tag="ACHBJ1_000651"
     CDS             complement(6884..7780)
                     /gene="murQ"
                     /locus_tag="ACHBJ1_000651"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM9429618.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQVKLAEANGFVKGTL"
     gene            complement(7783..8844)
                     /locus_tag="ACHBJ1_000652"
     CDS             complement(7783..8844)
                     /locus_tag="ACHBJ1_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM9429619.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9055..9582)
                     /locus_tag="ACHBJ1_000653"
     CDS             complement(9055..9582)
                     /locus_tag="ACHBJ1_000653"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9429620.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9649..11151)
                     /gene="eat(A)"
                     /locus_tag="ACHBJ1_000654"
     CDS             complement(9649..11151)
                     /gene="eat(A)"
                     /locus_tag="ACHBJ1_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9429621.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11409..12530)
                     /locus_tag="ACHBJ1_000655"
     CDS             complement(11409..12530)
                     /locus_tag="ACHBJ1_000655"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9429622.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12656..13597
                     /locus_tag="ACHBJ1_000656"
     CDS             12656..13597
                     /locus_tag="ACHBJ1_000656"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9429623.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13747..14643)
                     /locus_tag="ACHBJ1_000657"
     CDS             complement(13747..14643)
                     /locus_tag="ACHBJ1_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9429624.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14912..16252
                     /locus_tag="ACHBJ1_000658"
     CDS             14912..16252
                     /locus_tag="ACHBJ1_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9429625.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16470..16913
                     /locus_tag="ACHBJ1_000659"
     CDS             16470..16913
                     /locus_tag="ACHBJ1_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM9429626.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17417..18835)
                     /gene="nhaC"
                     /locus_tag="ACHBJ1_000660"
     CDS             complement(17417..18835)
                     /gene="nhaC"
                     /locus_tag="ACHBJ1_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9429627.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19238..20461)
                     /locus_tag="ACHBJ1_000661"
     CDS             complement(19238..20461)
                     /locus_tag="ACHBJ1_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9429628.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20492..21331)
                     /locus_tag="ACHBJ1_000662"
     CDS             complement(20492..21331)
                     /locus_tag="ACHBJ1_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9429629.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21348..22028)
                     /locus_tag="ACHBJ1_000663"
     CDS             complement(21348..22028)
                     /locus_tag="ACHBJ1_000663"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9429630.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(22030..23103)
                     /locus_tag="ACHBJ1_000664"
     CDS             complement(22030..23103)
                     /locus_tag="ACHBJ1_000664"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9429631.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23159..23397)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23440..23673)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23750..24421)
                     /locus_tag="ACHBJ1_000665"
     CDS             complement(23750..24421)
                     /locus_tag="ACHBJ1_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9429632.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24508..27156)
                     /gene="mgtA"
                     /locus_tag="ACHBJ1_000666"
     CDS             complement(24508..27156)
                     /gene="mgtA"
                     /locus_tag="ACHBJ1_000666"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM9429633.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(27498..27672)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            27845..28282
                     /gene="ndk"
                     /locus_tag="ACHBJ1_000667"
     CDS             27845..28282
                     /gene="ndk"
                     /locus_tag="ACHBJ1_000667"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM9429634.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28304..29170)
                     /locus_tag="ACHBJ1_000668"
     CDS             complement(28304..29170)
                     /locus_tag="ACHBJ1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM9429635.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29330..31423)
                     /locus_tag="ACHBJ1_000669"
     CDS             complement(29330..31423)
                     /locus_tag="ACHBJ1_000669"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9429636.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32172..32795)
                     /locus_tag="ACHBJ1_000670"
     CDS             complement(32172..32795)
                     /locus_tag="ACHBJ1_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429637.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32954..34351)
                     /locus_tag="ACHBJ1_000671"
     CDS             complement(32954..34351)
                     /locus_tag="ACHBJ1_000671"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9429638.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34383..35354)
                     /locus_tag="ACHBJ1_000672"
     CDS             complement(34383..35354)
                     /locus_tag="ACHBJ1_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9429639.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35728..36153
                     /locus_tag="ACHBJ1_000673"
     CDS             35728..36153
                     /locus_tag="ACHBJ1_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429640.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36641..37342)
                     /locus_tag="ACHBJ1_000674"
     CDS             complement(36641..37342)
                     /locus_tag="ACHBJ1_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9429641.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37358..37798)
                     /locus_tag="ACHBJ1_000675"
     CDS             complement(37358..37798)
                     /locus_tag="ACHBJ1_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM9429642.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37798..38373)
                     /locus_tag="ACHBJ1_000676"
     CDS             complement(37798..38373)
                     /locus_tag="ACHBJ1_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM9429643.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11112 a         8017 c         6688 g        12623 t
ORIGIN      
        1 agttgttttc tcacgaaata gacactattt ttaaatattt ttcgttattt ttttatcaca
       61 aaaattatct tacttattgt aaggtaaagc tttttttaat ttattgcccg ataatatata
      121 gaaataaggt ctagttactg ttttttttaa atgattaata aataacaact tctttctatg
      181 aaattgcaaa gcattcatct tcttttccgc taacgatata tccgtcagtg cttggtacac
      241 tttactaaaa agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga
      301 tatacaatct gccaccgggt aggataaaga ggatgatcat tccattaggt ctttgaaggg
      361 atgattttcc tctttttgtg tattttttta tagttaagga ggaatgtaag tatggcatgg
      421 atggaattaa tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt
      481 gatggttttt ctaaaatcaa ttattctttt tacacagtga ttggcatgat tgctagtttt
      541 tactttttgt caaaggcaat caaaagtttg ccgatgagtc tctcttatcc tatctggaca
      601 gggattggcg cagttggttc ggtcataatt ggtgtggtct tgtttcatga taagctgaat
      661 ctttccactt ggttctttgt ggggatgcta ctcatcagta tcattggcat caagattact
      721 agcggccact agacacttgg aataagtact gatcagaaaa aataagcctt tttatttaat
      781 attcaatttt tttatgaaaa aataagaaat aagtccaatg acctagcgac ttctccactc
      841 atcaggtgta tgcttgtact atctatagaa aaggagtgaa tatttttgaa aagtctttcg
      901 gtccaagaat tatccaagaa ctttggtcac aaacaagccc tcagcgatgt cagttttagc
      961 ttatcccctg gcaaaatcgt tggattagta ggtcctaatg gcgctggaaa aacaacgatc
     1021 atgaaagcta ttttaggact gatccattac tcagatggaa caatctgtat tggtgaaaat
     1081 gagatttctc cctctagtca taaagggttg gaacaagtag gagcattgat cgaatacccg
     1141 ggtatctatc cattcttaac aggctatgat catctgaagt tattctctga aaccaacgat
     1201 gtatctgcta tcgacactat cgtgaatcag ctcaagatgg aaaagtacat tcacaaaaaa
     1261 gcgaaatcct actcgttggg aatgaaacaa aaattgggaa ttgctcttgc cttactgaac
     1321 catccgtcta tcgttatttt agatgagccg atgaacggtt tggatccaca agcaacaaga
     1381 gatgtgcgaa aaatcattac cgacttggct actcagggca cctctttctt gatctccagt
     1441 cacttactca gcgagcttga aaaaatggtc gatgagctga tcctgatcaa tcaaggaaag
     1501 atcatcaaac aatgtacaat ggctgaattg aaccaaacag cgcaagattt catcgttatc
     1561 tctacaacaa atgatctagc tgctcgaacg atcttgacag aagctggata tactttggaa
     1621 gatcctgata aaatcaagct tttaaaagaa aacgaagaat tattaggaac cgtgatccag
     1681 ctattgtctg cacaaaatat ccatgtgaca gacgtccagc atctgacgaa tgatttggaa
     1741 tcttctctat tggaaattct ggattcagta aagggggaag aagaaaaatg acattgcttg
     1801 taaaacaaga attatttaaa ttgatcaaga aaaagtcaac tgctgtttta tctgttctat
     1861 tagttgtttt gctcatagga acagcacttc tagccaaaaa atacacgacg atcattgatc
     1921 cagttgaaat gactgctcag ctttttagtg ccacatcttg gattgtcttt atcatgattg
     1981 cagctgcaag cacgatcatt tcgatggaag cacaatatgg tacattgaaa aatctccttt
     2041 atcgaaagta ttctcgagga gaaatattag taagcaaatg gatcacatta gttatttatt
     2101 ctgtttattt atacctttta gctattattg tcactgtgtt gatgaaactg attcttttcc
     2161 cttcgatttc cttcacggag aaggtttcta ctggtcaaac actgatccaa agcttgttca
     2221 cttatacttt gggtagttat atcggtttat ggctgatttt aagtttagtg ttgatgattg
     2281 cttgctttat caatagttcc ggcgcatcca tctcagcagg aatcgtcttt tattttgctt
     2341 cgtcaatcat atctggtatc cttattgcac tcatccaaaa atgggagtgg atcaaatgga
     2401 atccgatcag tatgttgaat ctgcaaaacc aaattggaaa cgaagagatc atgaagcaat
     2461 tgactcatct ttctactaat caaatgctat tcggtaattt agcttacatc gttttgttct
     2521 tagcattagg atatctcgta tttaagcgaa aaaatgtatg atgactcatc aaaaaagagg
     2581 ctgtgaaaga gacgttcagc cttattgccg attggctgtc tcttaaacac cgtttattct
     2641 gaaaaaggag ctgtgacaac atttgtgtca cagctccttt cctagtatgt ttttgaataa
     2701 acgacaggct cacaacctct ctttatactt cttttatact tctatacgtg aagcatccca
     2761 gtggcaaata aaaccattgc aatcactccg accatcgtca cacctataag taaaatctta
     2821 tctaagtgga tatgttgtac aggctcgttt tgctccttac gtgctttgag gtaaaaataa
     2881 atgcctggca agaaaagaac aagaacaagc attacttcat taaaggctac gactagcata
     2941 gcccatactt gaaaggcact tgctaaaagc cctactaaaa attggaccca ttctttccgc
     3001 tgataagaaa gtttcatttg gtacaatcct aagaaaagcc acgtgaagaa aatagctgct
     3061 gtaccaagtg tgtaagcaaa gttataagca ctgtcagtga ataataatga aagtaaaaag
     3121 acattcgtac ataacgtcgt caatacgata gcataagttg gtgccccatt tttgttgagt
     3181 tttccccatg ctttaggcaa aagtccgtct tcagccatgt ttcccattgt ttcaatcggt
     3241 aagatcgtcc atgagagcca aacaccaact aaagaaatga tcaaaccgcc gttgatcacg
     3301 actgctcccc atttaccaac catcatttca aacacatagc ccatagcagg ctgcgccttg
     3361 atcgtttgca gctgattcat cgtcattgtc ccatatggaa gcatggaaac caagacgtaa
     3421 ataataatca agccgaatat ccctagaatc gttgctttcc caacatctga ttgttttttt
     3481 gctcgtgaac ctacgacaga ggctccttca ataccaacaa atacccaaag catcaccatg
     3541 atgcaactca atacttgact ggaaaacggg atcggctgtc cattcgcatt caatataatg
     3601 tcgctagaaa agttatcaat gaattgttgg atcgaaaacg tgctgatagc tactacaata
     3661 aataaaaata atggcactaa tttacagaat gtgacgatag cattgacgaa actagcattt
     3721 tcgactcccc gattcactag ccatgcatat ccccataaaa agactgtact catcacgatc
     3781 gatgccagat tttgcccacc ttcaaatact gggaagaaat atcctaatgc gctcatcaac
     3841 aatgttgcaa aagctacgtt gcctaaccag catgtgagcc agtaacccca accgctgata
     3901 aaaccagcga aacggccaaa accttcttct gcgtaactaa agatcccgcc ttttaaatct
     3961 ggacgttttt tattgatatt attgatcgac aaggagagca tcaacattgc tgttcctacg
     4021 agtacccaac tgaaaatagc tgggccgcca gccgatccgg agatgtccgt cataatacca
     4081 aatacgccac ctccaatcgc tgaaccaatt actaaggcag caagtggaat taaaccaatt
     4141 ccttttttct gttgctctgt atccatgtaa gcatcctcca aaataaaacc gcttttatca
     4201 aaaagtataa caaacctttt ttatttttac ttaattaata aaaactttaa tagaaatata
     4261 attctaccgg cttttttctc attatacaag caaagcgcct agtccctttc caaattacac
     4321 caccaaaaaa cttaaaaaag atgctatact aaaaggaaag aattggagga tgatccatga
     4381 acattttata tatatcgatt tcaggaaata cgcgtgcctt tgccaaacat ctagcagaat
     4441 atgctgaaaa aatgcatgca gaagatcctg tcaatcctga agttacttta aaagaaatcc
     4501 atgaaaatag tgattttacg aaagaaacag aaccattttt tactttcgtt cctacttatt
     4561 tagacggcgg aaatggttta gataacggtg atacagaaat cttgaccgaa acgatgcgtg
     4621 aatatctgga acatgaagac aatcataaac tttgtcttgg tgtagtcggt agcggaaata
     4681 aaaacttcaa taatcagtat tgtctaacag ccaaacaata tgcacaaaca tttggctttc
     4741 ctttcttggc agattatgaa ttacggggaa caccatctga tgtgacacga atttatcaga
     4801 ttttagcaga aaacaataaa taataaaaaa gaagaagctg aaaaatagat ttcagtttct
     4861 tctttcgttt ctattgtttc tatcatatta agctttgaca tccatcacag gtgcttttgc
     4921 ttttgcttta cctgtttttt ccaagacaaa gttttttaca cgatccatat tggttaccaa
     4981 gacgatcatg gatgtttctt tgcctgctgc tttgatcgct tctaagtcag cttctgctac
     5041 aagcgtatca ggtgtcactt gctgcccttc tgttactttc acatcaaatg gcgtaccgcc
     5101 taagtcaact gtgttgattc ccatatgaag caagatttct aaaccatttg gcgttgtgat
     5161 cccaatagcg tgtttcgttg ggaagacaga gctgactacc ccagtaactg gagaatagat
     5221 atgtccgttt tctggttcta caaagtagcc ttcacccatc atcttttgtg caaaaactgg
     5281 atcacttgct ttatctaaag aaacaactgt tccgtcagca actgatgaga ggatagtcac
     5341 actttcagct gaagtttgag gtactacttt ttcaggcact tcatttactg tggctgtttc
     5401 agtcgctaat tcttcttttt ctaaacggtc tttaggaata ccgaagaaga atgtagcaac
     5461 gaagccacca gcataagctg ccaataatcc aaggatgtag cctaaccatt taccgtcaga
     5521 gatcaatggg atcaaagcgg ctcctgaagg tccaatagcg atagccccga catttccgat
     5581 cattccgatc actgccccgc cgatacctcc accgatacaa gcagtaataa atgggcgtcc
     5641 tagaggtaat gttactccgt aaatcaatgg ttcgccgatt cctaggatcc cgactggtaa
     5701 agctcctttg atcatttctg ttaattcttt atcttttttg cagcgaatcc ataatgccaa
     5761 tgctgcaccc acttgtcctg caccagccat cgcaagaatt ggaagcaata gcgtacgacc
     5821 agtttcatca atcatttgca catggattgg agtcaaaatt tgatgcagtc cgaacataac
     5881 catcggcaag aataatgtgc ctagaacgaa gccggcaaat gctccaccaa cgccaagtac
     5941 ccagttgatc ccaccgacca tgccatcaga aatgaaacct gctaaaggca tgatgaagaa
     6001 aatttcaatc agaccgatgg caatcaatga aagagttggt gtaacgatga tatctaccgc
     6061 atcaggtact actttatgca gttttttctc aacgattgac aatagccaaa cagcgaagat
     6121 gacacctagg atcccacctt gtccagctgc aagagcttca ccagtaaata agtttttcag
     6181 tggtgcttcc ggattcatcc ctgtcagcat cacaacggcc ccgataactc caccaagagt
     6241 tggtgttgca ccaaaaactt gtgcagcatt gatacctgta tagataacta ggtagctaaa
     6301 catcccgttt ttcaagatgt tcatcacatc gatatattgt tgccatgttg ctgcatcaag
     6361 tgttccggct gtgactaagt ttgctaaaac tgcggcaatc cctgccacga tcccagtacc
     6421 gacaaatgca ggaatcattg gcacaaagat attagaaatg tcttttaata ccgatttgat
     6481 tttagatggt ttttgtttcg atttttgtgc cgctttcatt tcttgtgccc gttcgtttac
     6541 acggtctttt ccagatgctg atgaatttgc tgtagctgta cttacagaag aaagtttttc
     6601 tcctaatttc acgcctgcca aatcaaccat ttgctgtgct actttgttca ctttcccagg
     6661 tccgatgatg acttgaagct gttcatcttc tactacacca agcactcctg gaatcgcttt
     6721 taattcagag acatttacaa gatcatcatt gatgatcttc atacgcacac gagtcatgca
     6781 gtggacaatg ctttctacat tgcccatgcc gcctgcccct ttataaattt gttttgccag
     6841 ttcttccaaa gtcagttcgt tttttgccat ggtcttttcc ccctcataag gtccctttta
     6901 caaatccgtt tgcttcagct aattttactt gagcttcctc tttatcacta tttgtcaaaa
     6961 tcatgacgat agccagctta acattttgat ctgcttcttc aaagtacttt tcagctgttt
     7021 cgtaatcagc ttctgtcgct tgcatgatga ttcgttttga gcgttcgatt agtttttcat
     7081 ttgttggttt gacatcgacc atcaagttat tgaatacttt acctatacca atcatactga
     7141 tagtagaaat catattcaag ataagttttt gtgctgttcc tgacttcaat cgagtagagc
     7201 ctgtcaaaaa ttctggacct gcatcgactt cgattgggaa atccgcgtgt ttactgattt
     7261 cggcttgttt attgcaagaa atcgttcctg tgacagcacc gatcgatttt gcgtaatcta
     7321 atccaccgat cacatatggt gtacgaccgc tagctgcgat accaataact atatcatttt
     7381 ttgtcaaatt cagcttgatt agatcttctt gccctagctc ataagaatct tcggcccctt
     7441 cgacagctac agtcattgct tcttctccac cagcaattag acctatgacc atttccggtt
     7501 ctacaccgaa tgtcggtacg cattcagcag catctaacac acctagtctt ccacttgttc
     7561 cggctcccat ataaatcaga cgtccacctt ttttaaagga ctcaatcgtg ttagcaatca
     7621 ctggttcgat acttgtcagt tctttttcaa ccgcagcggc cactttctga tcttctttgt
     7681 tcattagttg aacggcttga gaaatactca tctcatctaa tccaaaagtt tcttggtttc
     7741 tgcgttccgt tgttaggttc tctaaattga ttttgctcat ttttatcttc cttcttttct
     7801 tataaattga tagttcatac cagctttgat ttggttgatc aatggtagct cttctttgat
     7861 aactttagct actacattca ctttttcatc cgctggcaaa tcatatttgg ttaattggat
     7921 ctcacccata tagcgcaaat acttttcgtt atctaaagta atagaccctt tcatacgcgt
     7981 agctgtattt cttgctggga taggcggaat ctctcgaaaa cgtgcatcta cgcttcgtat
     8041 gacatctctt gcatcgtctt gccgattcgt atgcttccct aatatatatt cgtaaaattc
     8101 ttcatctaca ggttccgtat ggagcaagac tgtctgttct ttttggaaag aagcaaattg
     8161 ctcctgaacg ttttctgaca gacctgcatc tccgatgtaa acaatatccg tgttacttgc
     8221 taatagatct agagcgcctg caagaggatg gatccctcga tgttcttcca aagttggcaa
     8281 tccttgatat agtgggcctc tcaactctgt atctcctggt acaaaacctt ggacaaggag
     8341 accttgaatt ttgagccatt gatttttttt ctgataccat tctttgtcca gccctgtttc
     8401 gggacgtgga taataattat gccaagcttc aatctgtgag aaatcagctt ctgcttcttt
     8461 caattcatcg atatctttag gcgtgatcgt gctagcattc aaactgattt tcaattgatg
     8521 tgaccagtcg gcaatctgct gattggaaat atgataatcc attcttaaac ctgttacacc
     8581 aattgctttc aaaggcgtta gatcttctaa tgaaaaaccc gctcgttcca atgcctctcc
     8641 tgaaatatcg accatcaatt ctagtttatt ctttttcgct tcgcttccta gttcgatcaa
     8701 tcgcttttga tatgccgaaa catcatcttc tggtatatgc atggaagtga aaataccgac
     8761 aaagccattg gatgccattt ggcggatata ctgtttcttc tcttctgaca aagaatcgtt
     8821 cataaatatt gaaaagccaa acatattatc cctctttttc tgtatccgtc ttcatttcta
     8881 tgactagagt atatatctaa agaaacaaaa tttcaatact tttattaaaa taattaaatt
     8941 tcatttctaa aggttgagtt tatattatca aaagaattgt cataacctaa aaaaggcact
     9001 gctaagcaaa agcagtgcct tgaaatattt tatttaaata acacaaaaac ataattactc
     9061 ataagaaacc aattctgtat tatctaccat gaatgtaaag aacccctcat taggcatcgg
     9121 atgaaaataa gcattgatca ctcttatcaa ggcattatgg caaactaaca gatagacatt
     9181 ttcttcgtct tcgatgcatt ctttcaataa aggaacgatc cgtgcataca catctaagac
     9241 tgactcccca tttgggaatc gtacagcgaa cgctaaacgt gctttttgaa agttctcatc
     9301 tttgcttggc agtccatcat aatccccaaa atccatttct accaatcgtt catcaacggt
     9361 cagcggcaag ctgagacgat cagcaactgc ttgtgctgta tctcttgctc gttgaagagg
     9421 ggaatggatg attttcgtga ttggaacagg caaatctact accttttctg ctagtttctg
     9481 tgcctgcatc tgtccttttt cagtcagcgg cacatctgcc cgtccgcaaa ctaatccttg
     9541 tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc atgtgattct ctccttcgtt
     9601 ggcaaagttc cccttaacca taccttgttg ttcttgagat tacaagggtt agattctctc
     9661 aagagaaata atctcagtag atactttatc caggaaggtt tgatcatgtt ccactagtaa
     9721 catggcaggt ttcacgttca agatcagttg ttctaattgt tcttgattga agacatccaa
     9781 ataattcaat ggttcatccc atgtatatag ttcagctggc tgtgataaag atttagccaa
     9841 ttccactttt ttccgttggc ccatactcat ctgctcgatc ttgttatgaa aaacatctct
     9901 ttccatccca agctttcgga ggttgttcaa aaatgcttgg tagtctactt ggtttttctc
     9961 tgcaaattcc gccaacgttc ctcgattgtc ttcataattt tggcttgcat aactaatgct
    10021 cagatgtttt ggctggtatt tttctcctat aaccttgccg ttgaatgccc ccagaagata
    10081 atggataatg gatgactttc ctgcaccgtt aggacctgaa atcgccaccc gctgatgagg
    10141 ctcgattgta aaatgaattg gctcgaataa cagattttca taccctaatt gaagatcttc
    10201 tacgcttaaa agtcgcttat ggtgagacgc ttggctattc atcgtcaaag aatcgatata
    10261 ttcgatatct tttaatagtt tttctttctc gctgatctgg gtctccatcc gattcacgat
    10321 tgctttggat cgtttcatcg tccgtgcagc atcagcaccc actgctcctt tattcactcg
    10381 tctagattca gtatcgatga atccgacttg tttctttgtt ttatctcctt ctcgggaacg
    10441 agaccattcg gctttttcag ctgctgtttt ctttagccta ctgacttctt ttttcaattt
    10501 ttcattttga gccatttcaa attcatcacg aagttttttc tgttcttcat agatagagaa
    10561 attcccttga taaagttcca gttgactttt ttcgattgct aaaacatggt ccactacttc
    10621 atcgataaat ccccggtcat ggctgaccac gatgaagcct tgttttttct ttttcaaata
    10681 agccgctact tgttttctac cagagatatc caaatgattc gttggttcat cgattaacgg
    10741 gaaatgagtg tcatccacaa ataaaagtgc cagtaggact tttgtcttct ctccccccga
    10801 tagtgtgctg aattctctcc ataagatttc aggatctgtt tgcatcaatt ggagctctct
    10861 ttcgatttcc catatctcaa aatccgtaat atcatttaac acgtaatagg ttaaacgttc
    10921 tttatctttt gtctgttgcg ggaaataggc aaattcttgc tgatggatta cttgcccttg
    10981 ataaggtagt ttgttttgta gaatattcag taaagttgtc tttcctcgac cgtttcgtcc
    11041 gataagtcct agtttccatt gtgtgtcaaa atttagattt gcttgttcaa ataataatgt
    11101 gccttggctg tcgtagccga atgtcagatt ttttatttcg attttagaca ttttccatca
    11161 cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc aagggttgga
    11221 tacacacaaa aagacccata catcgaaaaa accaagccaa tcccgaaaat ctgcacactg
    11281 cttccttgtc tccacaaggt acagtctgaa tgcagctttc atcaatcgac ttaatttttc
    11341 aatgcacgga tccctcactt tgtcctatat ttgaaattac gatagcataa agaaaatgat
    11401 tttgtcaact acttcgggag gatggtgaaa aatctgctaa gtgtctgtcc taagattttt
    11461 gttttttcta atgtttgcct gtgttccatt gcttcttcta aacctattgg acccaaatga
    11521 atacttaaga caattgggag aaaatcagaa agtccttcta gctcttggtc tctacttccg
    11581 caaatagcga ttattggttt tcgatatttt tttgcaagca ctgctacacc gtatggaact
    11641 tttccgtgga tcgtttgctg atcgatccgc ccttctcctg taatgatgac atctgcttgt
    11701 tcgatctttt tttccaatcc agtgatttgg ctgactaaat cgaaaccaga aaccatcttc
    11761 gctcccaaca aaaaaagggc tccaccaata ccgcctgctg ctccacttcc tggaatatca
    11821 tctaaatcca cctcccagaa atcttttatt tgctgagcaa tctttcctgc ttgtttatct
    11881 aagaaagcta gcgtcgtatc tattcctcct ttttgtcttc caaattgcca agcagctcct
    11941 tgttgacctg tatatggatt ggtaacatct gcagcgataa tcaattttac tttagaaaca
    12001 agaacgtttc ttcgatcaaa atgaagtttt ttagtagata ataaaggatt tccttctgtg
    12061 tacccttcta aagaagcacc caaggattgg agtaatccta gaccaccatc gctacacccg
    12121 cttcctccta aagtgacaat aatttgttct acttcttgaa gcagtgcatc tttaataagt
    12181 tctcctaagg cataactgga agcaagtcgg atagtttgat ccgttggttt atcaagtaga
    12241 tctaacccca atgcttgtgc agattcgatg actgcggtgt tttttccttg ccaagttgtc
    12301 aataaatagc tgatttttcg ctgacggaat aagagatcca tcccatcaat ggtacgccat
    12361 gttcccctga gcgcgtaata gattgcttct gcagtatttt ctccgccatc tgcaatcggc
    12421 acattgattt ttcttccttt acgatcttcc cagccattta gaactgcctg atttagttca
    12481 aatgaagaag cacttccttt aaatgaatca attgccgcca aaatatccat ctgtatccct
    12541 cactttcttc ttaagtaaga cacttttttt catttttttc aaacaaaaca atgctatttt
    12601 cttccatttt tctatatact aaaagggaac tcattgaata aaggagagaa aaaacatgaa
    12661 aatcgcaata gctggggccg gagcaatggg cagccgaatc ggattgatgc tacatcaaag
    12721 cggaaatgag gtcctcttga tcgatcgttg gcctgctcac attgaagcca ttcgtacaaa
    12781 tgggttgatt gctgacttta acggtaaaga agtggtcgca aaactgccaa tctattcacc
    12841 agaagaaatc atcgaaagca acgaacacgt ggacttgatc gtagcattaa caaaagccaa
    12901 tcaattagat gatatgtttt gttcgatcca atccattatt actgacaata cttatgtttt
    12961 gtgtttacta aatggattag gtcatgaaga tgtactggaa aaatatgtgc caaagaaaaa
    13021 tatcttattt gggatcacga tgtggacagc tggtctagct ggtcccggaa aagtcacatt
    13081 gttaggtgat ggagaaatcg aactagagaa cttggaacca gaaggcgaag catttacaaa
    13141 aaaagttgta gaagtctttc aagaagcaaa tttaaatccg atctatagcc ataacgttcg
    13201 ttattccatt tggcgaaaag cttgtgtaaa tggaacattg aatggtttat gcacgatttt
    13261 ggattgtaat attgctgaac taggcgcgca aaaagcggca gagagcatgg ttcgaacaat
    13321 cgtatctgaa tttgcttcta tcgcagcaaa agaaggaatc atactagatc aagaagaagt
    13381 gtaccaacat attgcttcta cttatgatcc tgacaacatc gggttgcatt acccatctat
    13441 gtaccaagac ctgatcaaaa accatcgttt gactgaaatc gactacatca atggtgccat
    13501 ttggcgaaaa ggacaaaaat atgatattgc tacaccatat tgtgcctttt tgacccaatt
    13561 agtccatgca aaagaaggaa tccttggcgc agaataaaag gctctctgat gagaatcagc
    13621 tgaatcagca gctggttctc tttttttaag cccaataaaa gatcaaaaag aaaacacaac
    13681 tctcatgatg ccataagaat tgtgcttgtt tgtttcagta cataatctct tcaaaatgta
    13741 ggttttttat ttgttttcaa cagaggagag gtcatattcg tattgctcat aaatagtgga
    13801 atcgttagtg ataaatcgtt ttgcccaatt ttctgcatca taaaattcca atagctctga
    13861 taggattggc ttaaatggtg tatgcagaaa ttccggtaat tcatctaaat cacgttctcg
    13921 aagcaaaaaa atattttcag gacgataaaa atgataatga gcaatcgctt gattatccgt
    13981 cactagtttc ttttgaaaat acatggattc aaatgtccgc aaagtcactc cttgctgacc
    14041 ctcccgcaac agttccagaa tcccgcgcga ttcccctgtt tttttcaggt actcttcata
    14101 agtaagatat ccaggttgtt cattccctcg atgtggaaga atgaaatagt tggtcgtaac
    14161 atctcttttt ataaattcct gcttatagct ttctgctcgt tcttttctcc cattattcgt
    14221 tgcaccaaaa aatagatccg tctttggttc gctagaagaa aaattcatat gtttcgaata
    14281 aaatgaacta ttatgtttca gcccaagttc ttttgcttcc aaaaaatcaa aatgataaat
    14341 ctcatcgatt gccggttcat cttgtgcctg cttcagcatc gcctcgtgtt ctgctttgaa
    14401 gtgattccaa aaaaataaca caatttttgc attggtaaat gggcgcacgc gtcgagccat
    14461 ctccatatca aatccataat ctaaaaaaac gatcagctga taatttgcca actgttgtct
    14521 agctgattta atgaattccg actgtagtaa atcttttccg gaccaaacat ctaaagagcg
    14581 gatggaaggc aaaaaataaa gtgatttatt ttttgcaact agcagtacgt catttttgtt
    14641 catcttttcc gcctcacttt ctctgtcact ttttcctatg atagcctgtt taaatagtaa
    14701 aaagccagta aaacgtttcc tatatttgta tgaaaattta acatttgtat gaaaatatga
    14761 tgaaataaat cattcttcta ttaaaattcg cctaaaagtt ttctgaatag ttgtaattgt
    14821 ctgaaaattt gttatcatta gtcattgtta aatattgcta tttattctta atataaattt
    14881 tgattgtgaa tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact
    14941 tagctccaga catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa
    15001 aggaagtggc gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg
    15061 tgggctgatt ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga
    15121 cacgcatcac ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt
    15181 tactgattgg gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc
    15241 tgcttccttt ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt
    15301 ggctgtttta actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata
    15361 ttggctggct tttgctaaaa tcagtgtcat tatcttactg atcatttttg ctacttatct
    15421 tgtcggtcag caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca
    15481 tggtgggttt actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta
    15541 ttcttacggc ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg
    15601 tgcgattcct aaagcaatca aaggtgtcat gggaagaatc atttcctttt acatcattcc
    15661 tttgtttttg cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc
    15721 ttttgtgatg gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt
    15781 gattgtcctt gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt
    15841 gttcttccgt ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca
    15901 tcaagtaccg caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct
    15961 ttcttatttt gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt
    16021 attattgatc tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac
    16081 attatgggat aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg
    16141 tatcctgttc acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat
    16201 ttatttcagt tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata
    16261 gaaaatttta ttttatttat gtggaataga tggagctatt gaagaaagga gttgtgacaa
    16321 aagtcttgtt gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg
    16381 ttcaaaaacc ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga
    16441 aaggaatgaa aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga
    16501 tcggtgtttc aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc
    16561 atatcttctt cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag
    16621 tcatcatgct gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct
    16681 tagccgctag tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg
    16741 tcaacgacac agcttggatg aaggccatga ttcctcatca ttctatcgct atcctggcaa
    16801 gtaaacgagc tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcattcagg
    16861 cacaagaaga agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat
    16921 ccgtccaaat aaaaaaagag gatgttccag aagttatcag cttcaagaaa taagaagaag
    16981 ctacttctgg aacgccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa
    17041 gaacagccaa acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga
    17101 tgcaagcttt tgaacaccgt ttattaagag gttgtaaaat cagcgttctg acctgagtat
    17161 tagagagaca aatggaaaaa tagttcctca tattttgcca ttttttgagc taataccgag
    17221 ggtcagctga ttgaacaccg tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta
    17281 ttaagaaaaa ttttgtgaaa tggctttcca catttttgta taaatttggc ttaataccga
    17341 gaaactggct tttgaatacc gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca
    17401 atcccttttt atgtttttat tgttctaatt gcgctgtctt gcttactgct tcttgcggtt
    17461 tcacaaattt ttttgagatt ccccaacctg tgaaggcact gaccatcgaa aacacaggag
    17521 aaagcaaact gaaaaatacg aatggcaagt aagtgagtgt aggaatacca aatgtacttg
    17581 ctacgaaact tccggcaatt ccccaaggca ctaagtagtt gattactgtt ccaccatctt
    17641 ctagcgttct acctaataca gttggatcaa atcctgcttt ttcataagtt tctttaaatg
    17701 cattaccagg cagaataaca gataggaatt gttcaccaat aaaaatattt actccgatac
    17761 ttgtcatcaa tgttgtaaaa atcaaatttg gtgtgttcgt catctttctt gatactcgtt
    17821 ccatcacggt tgagatcaag ccaaattcga tcaacaatcc acctaatgac aaagttagaa
    17881 taatcaatga cacagtaggc atcatacttt cgattcctcc gcggctaagc agtgaatcga
    17941 tttgttggtt tccagtattt gaaacaaacc ctgattcgat gaccccagcc aacttcgtta
    18001 aggaagtttg tgggttttga ataaaaatca ttcctactgc tacagcgata ttcaacaaaa
    18061 tcgttaaaat tgccggcacc tttttccatg cacacagtaa cattaaaaat aaaggaatca
    18121 gtgaccagat tgaaatagta aagtttgctt ctagaacatc agtcacttca cgaatagatg
    18181 aaagacttgc ggctttactt gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa
    18241 atgccgggac agttgaccac atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa
    18301 tagctgctgc tagatttgtt gattcggata atggagacat cttatctcca aaaacggcac
    18361 ctgaaacaat agctccaaca accagcgctg gatttaagcc tagtgttgtc ccaataccga
    18421 aaaatgcaat cccaatcgtt gacataacag taaaggcact ccctacagcg ctacctacga
    18481 ttgcacacac aacaaataca gaagggacaa accactggac actgatcaat ttgaacccaa
    18541 tgaccattag agttggaatg atccctgctt gtatccatac agcaatcaat gaaccgacta
    18601 aaataaaaat aaagattggg atgattcctg gtttgatgcc attgatgatt ccttcgtgca
    18661 gccaatctgt cgggtatctt tttatcactg catacaacat gaccattccg atcgctgcta
    18721 agatcgtgat attaggtgag acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa
    18781 taaccattaa tacaagtgtt gcttctaaaa aacttggtgt tccttttttt cccatgataa
    18841 tctctccttt tttagttgtt cttatgttct aaaaaagtta agatatcgga ttactccgcc
    18901 gcaattcatc cgcgattcct cctttataaa aataaaaaga cctgttttaa actaaggacg
    18961 aatgactccg cggtaccacc ttaattaaaa acagatctgt ttttcacttt caaactttta
    19021 ttcaaatgta tggactttcc atgtaattcg tcaattaggc ttgatcggtt cacagcgacc
    19081 accgactttc tgaacaaaac acctaaccgc tactgcgtga aaagttgact tgtacgaagt
    19141 ttaccttcca taagatcatt tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa
    19201 ttatttttat aactttcaca cacaagttta ttttttacta gaaagtagcg ccctcttgtt
    19261 tttttcttgc tactatttct tgatgttctt gcttgattct ttctaatagc tttggctctt
    19321 tcaacagatc aagagctgta ttggcgagta gttctgctgc aatcgcaata gaagcaaggc
    19381 ctttgtcact tttagcagca gctttgaatt cttctgtgtg tccggcaata tatgtctcag
    19441 agattgctac cgtaggctgg attgttggta tgacttggct gacatttcct acatcagaag
    19501 aaccgagact tgttctttgt tccgtttcta tttcttcatc tggaacacct gcttcttttg
    19561 catgctggaa gaataagtca tcaaaagaag gagtaacgat gacatcatca actgcatttt
    19621 ggaacaatcc aaactcaaaa gtcgtgcccg tacttatcgc agcacctcgt acgatatttt
    19681 ccactttttc atatacttta tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc
    19741 cagcagcgta atctggaatg acgttggcag ctactcctcc gtctgtaatg atcccatgga
    19801 tattgacatc ttttggcaat tgcaagcgta gagcgttgat tccattaaac acttggatca
    19861 atgcttccaa agcatttact cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt
    19921 aaaatttgat gtcgactgga tcattcgcta aagattctgt cgttttccca taacgaaaag
    19981 ctggatggac gcataacgct acatcgacat cctcaaaaaa accttctcga acaaagctgc
    20041 ctttagctga tccgttctcg cccccttctt cccctggggt gccatataca cggatttctc
    20101 caccagtttc atcaatgaat tgcttcacta tgctggcggc taatacggag taagtaccaa
    20161 acaaattatg cccgcatgca tgaccaatac ctggtaaagc atcgaattct gccaaaaagg
    20221 caattgtggg accgggttta ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag
    20281 ctacttcttt ttctacccaa aacctttctt tttctaattg tttaatcaac gctgctgagg
    20341 aataaaactc atagttactt acttcaggat gttcgtggat atctagagca agtgcttgat
    20401 attctggtaa acgatattgg attagatcct tcaattggtt tgttgagatt gttgttgtca
    20461 taaaaaattc ctcctatgct agtcattttt cttattttct cccgaatgtt tcccatgcag
    20521 gcacgcttga tccttttgat gttttctcga ttactttgac tgtgtcttct gtttgataag
    20581 cgtctactat tttttgatac gttttattgt tttcatcttc ttttcttgca acgatgatat
    20641 tcacataagg acgtgcatgg tcatccactg gttctaagaa gatggcatca gaagcaggat
    20701 taaatccagc atctacagct acaccgctat tgatcactga aatgtctaca tctgacaaag
    20761 cacgtgctgt ttgggaagca tctaattcag aaatgtctag attttttcta ttctcggtaa
    20821 tatcagaaac agtgggcgct tgtccttttg ccttatccac tttgatcaat ccagcagttt
    20881 gcaataacag aagagctctg ccgccatttg taacatcatt agggattgct acttttgctc
    20941 cgtctttgat atcagctaca tccttcaaag tgttagaata aattcccagc ggtgcattga
    21001 cagtatttcc gatcgatact aaatcagttc cgtgttcttt attgtaattg tctaaaaaga
    21061 attgatgttg gaaagaattc aaatcaatcg atccatctgc taaagccaca tttggttgat
    21121 tgtaatctgt aaatttcacg tattccaaat taatgttctc ctttttcaaa cggtctttca
    21181 cgttgtccca tacatctgta tcttctccaa taatccctag ttttacgata gtttcttttt
    21241 tgtcagttgt atctgctttt ccttggctac accctgccaa ccaaataatt gctacaaatg
    21301 taatgactac aaaacgaata atttttttca ttttttctcc tccactttca atgtgtaatt
    21361 tttttaatca gttgattact gataaattga cttaaaaaaa ctaagattag aatcaataat
    21421 gtagccacaa tcgttacatc tgtttggaat cgattatacc ctctcgatat agcaagattc
    21481 cctaatccgc ctgcacctac tgctccagcc atggtagtca gtccgatcac gttgatgata
    21541 gtcaacgccg agacccgaat gataccaggc aaaccttcta acaaatacac acgtaaaatg
    21601 ataccgattg gactggttcc catcgcttct gccgcttcga tcaccccagg atcaacttct
    21661 agcagtgcgt tctcgatttg acgagcaaaa aacggaacaa ctccaaaaat caatggcact
    21721 aaagctgctt tcgcaccaat cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga
    21781 gaaagcaaga taatgaaagg gatggaacga atgatattga tcagcttatc aagtatttca
    21841 aatagcagac ggttagctag taatccatta gggcgtgtca ctactaacgt cacacctaag
    21901 atgattccaa tcatcccgcc aatgaatgct gtccaaaaag acatatatag tgtttgcttc
    21961 gtcgcttcga caaattcatc cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa
    22021 tcagtcattt tatcttcctc cttcgatcac tttgatacga ctatctaaat cgtttttttg
    22081 taaaatattt atttttacac cagatttgat caaataatcc aatgcttttt tccgctgttt
    22141 ggtttctcca gctaaagtaa cgatcaaact ccctaacggc gtttcttgta ataactctac
    22201 attgccatat aaaatattcg ctgttacttg atatttgctg tacagatgag cgattagagg
    22261 ttcgttcgtc tgatttccaa tataacttaa ttccaccagc catttattgg cgatctgatc
    22321 ggcaaaagca gaatgggcaa tgatcgtttc taatgcttgg tcaagatgag tagctgttcg
    22381 aataaaatct ttggtcaatt cttcttcagg attgctgaag atctggatgc tgctaccttt
    22441 ttcaacgatt cttccatctt ccataaccgc taccttgttg cagatttctt tgacaacttg
    22501 catctcatga gtgatcagga cgattgttaa acctaactgg cgattcaatt tttttaacaa
    22561 tgcaaggatc tgaagggtcg ttttcggatc aagtgcactg gttgcttcat cacataataa
    22621 taccttaggt tcattcgcca aagctcgtgc gattgctact cgttgttttt gtccccccga
    22681 cagttgttta ggataagcag atgctttttc ttccaaaccg acaagttcca gtagttcatt
    22741 cactttttga cgacgttcct gtttcgattt tccagaatac tttaaagaaa aatcaacgtt
    22801 gtcaaaaata gttcggcttt ccattagatt gaaatgctga aagatcatac cgatggcctt
    22861 tctttctttt cgcaattctt tgggagagag ctgactcaga ttcgtctgat tgacaatgac
    22921 ttctcctgct gttggtttct gcagcaaatt catcacacga actaacgtac ttttccctgc
    22981 acctgagtaa ccgacgattc cataaacatc gcctttttca atcgttaaat ctacagaatc
    23041 tactgcctgt atctcttttt ctttttgttt gaaacataca gaaacatctc gcaattcaat
    23101 cattaaaatt cctccttatc taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct
    23161 tgaggacgct tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct
    23221 cttcaagtac gatcatactc tagcaagata tcggtcgcag ccgtcatcgc cttacatcta
    23281 tgctcgggat gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc
    23341 taccgaagct ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat
    23401 tcaaaaaaaa agtcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg
    23461 taccaccttt tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg
    23521 cgttcgtttt aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca
    23581 aggccatttt cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt
    23641 ttccaaactt actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat
    23701 gaggaaaact gaaaagtcaa gtacgaaatt caaattattt tttctatatt tatttttctg
    23761 tttttttatt taacatttca ataacagttc gaagatcttt tccttccgca agaagtttca
    23821 atttttcctt ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa
    23881 cgcctaatcc tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg
    23941 ctgaactagc ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct
    24001 tgttctccga cacttcgtct ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt
    24061 gatccaagat atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat
    24121 gacgttcttt taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc
    24181 ttgtcagctg ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag
    24241 ggctacctgc tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag
    24301 cgtcaacgat ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat
    24361 cacgatacgt tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca
    24421 tctatacatt aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaaga
    24481 acagaagcaa agaaccagcc ttctcgttta taagagttct tggaatcgac ggatatagat
    24541 tttcttcaca gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca
    24601 gtaattgcca ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac
    24661 agaaccgaca ataattccta atgtagtgat aacaaacatg gcaaatgttg cgttgctttg
    24721 gataaatggt actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga
    24781 ttcgacaaac catcctgcat ggaataatgc gatgaaaatc actcgctgtg aactatccaa
    24841 tgtatggaaa cttccgccca ctacagcagg acaaataaca aaatacatca acaagtaagt
    24901 agtaatatca aatatcgaac ttgttggacc aaagtaaacc atgaattccc caatactaga
    24961 tgcattccat ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga
    25021 aatgcaggaa acatcataga tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa
    25081 aaatggcagg aacgtacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc
    25141 tgtagccttg atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct
    25201 ttccaaaatc gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc
    25261 cacagctgta tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat
    25321 accatctcct aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc
    25381 tttttgctga ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc
    25441 atgatcactc atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac
    25501 ttgacggcac actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac
    25561 accatgttct ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa
    25621 agcaagataa ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac
    25681 gcttggattg gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg
    25741 tacttttgca agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag
    25801 atcaataaat ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt
    25861 tttaccagct ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat
    25921 ttcatccact ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat
    25981 gatagccaca tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg
    26041 gcgcaaaaca cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt
    26101 atcttgtgtc aatgtccccg ttttatccgt acataacaca tcgattgcac caaagttctg
    26161 gatggcattc aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac
    26221 caaattcgtc gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag
    26281 tccaaaaaga aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc
    26341 tggagccatc aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc
    26401 aaagcttgat tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc
    26461 atttccaaca gaaacaatga ttccttccgc acttccactg atgacattac ttcccataaa
    26521 gacaagattt tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt
    26581 ttctactgga tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga
    26641 caataaacgc atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca
    26701 gacgatttct tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac
    26761 tttcacaagt gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt
    26821 cattgtcccg ctgacgataa ccatagtgaa gataatggct acaccgaaaa gatctttatc
    26881 tccaggagct gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac
    26941 taatgtaaat ggcgtgatgt atgctttcaa tatttctaca atgaatggtg tttttttgcc
    27001 atatgaaatc gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa
    27061 tccatttgct gatgcatgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc
    27121 tgcatagatt ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa
    27181 agacagctgt tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac
    27241 tgaaaggggc tgagaacaga tagataatag ccctggcctt tcctctagat ccttgttttt
    27301 cgaatgactg tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt
    27361 ccaatcattt ttcgaatagt tatttttcca attagaccac ctccttttta tttttcaccg
    27421 atggggattt gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa
    27481 gcatattaaa aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa
    27541 agcagatacc tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac
    27601 ccattggcgt ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta
    27661 acaaatctta ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca
    27721 attttccatg tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg
    27781 ttttatttgt ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc
    27841 tgttatggaa agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg
    27901 aagcatcatc caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac
    27961 tatgactcct gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga
    28021 tgaactgatc gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga
    28081 agtgatcgat atcgtccgta agatggttgg agcaacaaaa gctgccgatg ctgttccagg
    28141 tacgatccga ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc
    28201 ccgtgatgct gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc
    28261 gtctgaaaaa gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag
    28321 caatggccag cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga
    28381 gcactgtgaa gaattcttgg tagtctttcg tcttttcttc ccctaaagca gcagaaatca
    28441 atctttgata ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg
    28501 ataagctccg tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta
    28561 tataaaacga agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact
    28621 gtgctaatcg tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac
    28681 tgaatatcca aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct
    28741 gcatcaagta ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag
    28801 atacttctgt tttttcgacc atgtgaagat tcaaaaccaa ataatacaga tcttctgttt
    28861 tcttaaaatg cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta
    28921 cccgatctag cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca
    28981 tccattcata ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa
    29041 atgacatttt tttcactgaa tttgtctcaa tcccgactag agaagtctta aaacagattc
    29101 cttccgctaa ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac
    29161 catcatacat aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac
    29221 gttaaaaagg aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa
    29281 ccttcaagca ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat
    29341 ttttttgctg ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattac
    29401 cattttttga aaagacactt gtcgaatgtt cgcctttttg cttttcgatt gcttcattac
    29461 gttccgcttc atttttatag tcatattttt tcggatcaat cgtctcaaat ccgtttggca
    29521 cataaaaccg caagagattt tcctgattca gcttatctga aagacttaac gcagtcttga
    29581 ctttttcctg atctttggag atttcctcat cgattcctgc tgctttcgta tcgatgattt
    29641 ctcctgtctg attattgtaa gcttttcctc ctaggacggt gtatttgggt gtcacaaagt
    29701 tcccatttcg aaaagcaact acttgatcat gctgagaaga taataagtct gtaccaaaat
    29761 gaagataatc tttatcatcg attcccaata gatgtagtaa tgtcggcaaa acatcgattt
    29821 ctccaccgta ttcatgattg actgtccctt tagtataacc aggcatatgg atcatcaatg
    29881 gcactcgttg catctgtgcg ttgtcaaatt catcccatgt atctggatct ttaccaagag
    29941 cgcttgctaa atctttatta tctgtattcg agatcccaaa atgatccccg taaatcacaa
    30001 agattgaatt ttggtaaata ccagattttt tcaaatacgt aaaaaattgt tccaatgatt
    30061 gatccagata atgtgccgtt cgaacataat tatctactgt gctattgcca gtattcaaag
    30121 atggaaagtc gaaattcttg tcatccgtat aataaggcgt gtggttggtt acggaaagaa
    30181 atttcgtgta aaacggctgt tgtagatgct caagatattg ggcagattct ctgaataaat
    30241 ctttatctaa tatcccataa cctaacgttt cgtcggattc atcaaaataa gatcgatcaa
    30301 agaaattgtc ataacctaag tttttatata catgatctct attccaaaaa ctagctacat
    30361 tcccatggaa aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt
    30421 gaaagacatt gtctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc
    30481 cagtttccaa catattttca gcatcacttg tctttccctg tcctacttca tgaaagaaat
    30541 tgtcaaagct gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt
    30601 gtccgtctac cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca
    30661 catttttccc tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg
    30721 tataatctaa aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca
    30781 ttcctgtttt gattccgtca taaatagtat acgcatcgat tcctagatat ttgacaatat
    30841 atgaacggtc aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat
    30901 ttcctgaaaa aataagccct gaaagacagg taacagcgat agccatcggt ttgccgacag
    30961 ggttgctttt gataggtatt ttcttgacct tcaaccaaac cagtagccca atgaacacag
    31021 cgatatccag ccagtagatg acatcatggg gcttcatcaa agaaaaggag cttcctgaca
    31081 atccttgcga cactttcgaa aagcctaata cagatttgac tgtaatgaaa tctgtgaatt
    31141 cccgataaaa aataatattc aagtacaaca aaagcgtatt taaaatatca atgatcaaaa
    31201 gaatagctaa cgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg
    31261 tagtcgcaat tgggttgatc cacaagataa agtactggat cgtaccttcc acacctaatg
    31321 aaaaatctgc atagtaggca atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa
    31381 cgattgctaa acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct
    31441 attcacattt tttcttattt tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc
    31501 ttaagatttt tttattcttt ctaaagatta ttccgtccac ttttcacagc ataaaaccgc
    31561 tgcgatgtaa gcgattaaac taatgaagag ataaaagtct tgtggtgacc gcttcactcc
    31621 acggtattca aaagccagtt tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc
    31681 ttgtcgcagc tccttatttc ttgatgcagg acagcttttt cacaacttct tccttaaacg
    31741 gtgttctcct gccaactcct cggccacaag tcacaatatt tataaaacat gagaagctgt
    31801 tttttgaaat tgttccttgt ggctcagagt taaacggcag gctcaagacc tcttgataca
    31861 cggtgttcaa tcagctgacc ctcggtatta gattatgtca ggacgctaat tttacaacct
    31921 cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
    31981 ataaggagct attttttgaa attgctcctt gtggctcaga gttaaacggc aggctcaaga
    32041 cctcttgata cacggtgttc aatcagctga ccttcggtat tagctcaaaa aacggcaaaa
    32101 tatgaggaac tattttgccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca
    32161 acctctgttt ttcattgtcc ataggattga tattgtcctt gtgatgactc attcgtaata
    32221 attgaggctt gattttcttc acttcccgtt atcgtttgtc cactgccaga atctgaagga
    32281 cttgttcctg tcgactcgtt tttttgagaa gagacagttg ttttcttgac ttgttcattt
    32341 gtctttttcc attccgctaa acgatcggcg accacttttt tgtctacttt ttcagaataa
    32401 gcggtgatca cttttccatt tttcaaacgg gctactttgt cgccatcatg agtcaaaagc
    32461 aaaacggtca aatgcttgtc tttaaattga tattgtctga gagaaatcgc actaagtacg
    32521 tcatcgatat taataaattg attttcttct tttccttttg gcaaataaat gtccaattcg
    32581 cctttttcgt aattggcagt tattttatac tccgcccacc aataagttga gaattctttt
    32641 ttgaaattat ctactgattc tttgcttgct acgatttttt ctgtttcttt ctgagcaaga
    32701 tctgctgtca tttttcctga aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat
    32761 aaccaaaaga gaagaaacgg tctttttttc ctcatgcttt cctccactcc tatttaattt
    32821 tgactattct attatataca aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt
    32881 aaataattga acttcttttt cacaaaatgg taagaatgga gaatttcacg catttgctct
    32941 caactattct ttcttaagcc tcaaatccgt ttttatctga tacgtctttg aaccaatgac
    33001 ccgatttttt gattgttctt tttccatctt gtgctaagtc tactgaaata aagccatatc
    33061 gattcttata tgcattggtc cacgaccagt tatccataca tgtccacata tgatagccta
    33121 aacaattggc accttcttga atggcttgat ggacatattt cagatgagat tggacaaatt
    33181 cgatccgata atcatcgtgg atcatgccat cttctttgat aaatcgtgct tcgttttcta
    33241 cacccatacc gttttcagaa ataaagcagt cgatatttcc ataatgatct cgtacattga
    33301 tcaaaatatc atagattcct tgttcataga tctcccatcc gcgataagga ttcattttac
    33361 gaccaggcat ttcataattg tcgaaataat cctcaggcat tggaccgttt ttagtatcga
    33421 ttggtgtttc ttttgctttt acccggcgtg gctggtaata attgatacct aaaagatcta
    33481 ctgtattttc tttgataacc gtcaaatcct ctgcatggat cacaggcatg agatcaagtt
    33541 cttttactat ctcaatcaat tcacttggaa actcgccttt tacagctgga tcaaggaatg
    33601 aacgattgaa gaacgcatct gctatttgcg ctgctttaac atcttcaggg tttgattcgt
    33661 ctcttggata gcttggtgtc agattcaaga tgataccaat aacaccacct tgctccattt
    33721 catggtaaga tttgatggct aacgctgaag ccaacgcttc atgatagcca acttggatcg
    33781 cttttttcat atctgactct tctggataat gccatcgata taaataacct gcttcgactg
    33841 gaacgattgg ttcattatga gtaaaccatt tttttacttt cgaaccaaac aattcaaaac
    33901 aacttttggc atatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt
    33961 cttgcagata catcggcata tcgaaatgat acagattcac aaagggttcg ataccgtttt
    34021 caatcaactc atcaaaataa cgatgataga aacgaacggc ttcttcgttg attgttccag
    34081 attcatcagg tatcaaacgg ctccattgga tagatgttcg aaatgaatta tgcccagtct
    34141 gtttcatcaa ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc
    34201 ctacttgatc aaagaacttt tgcggttctt ttttgtacca ataatcaaaa atgttctctc
    34261 cttttccatc gcctgaaatc cttccttctg tttgtgggcc acttgctgca gaaccccacc
    34321 aaaaatttgc tggaaattga tattttttca tcaaagcacc tctttttctt ttttagttcg
    34381 ttttagttga ttccatgtat gatattttcc atcacaatca ctttttcatt ttcttcctct
    34441 tcttttttga ttcgttttaa cagctgttct gctgctcgtt ccccgatttt atctgttggt
    34501 ggcgtgatcg tgattttcgg aaggatactc agatcgtttt tatctaaata atcatagtga
    34561 ctgaacgcaa tatccttccc gattctgaca ccttcttgat tgaaacattc gatggcacca
    34621 atcgacatat tgtaattgct gacaaaaata gcagttgccc ctttttctaa taatgctttt
    34681 gccccgtaat agccgctttt tctagaatag tctcctgtaa agacctctaa ttggatcgat
    34741 ggatcatttt ggaccgcttc tttggctcct gctaaacgtt ctcttgctac gtagtctgtt
    34801 tgagtggcag caataaatcc gattttttta tgtccttgtt caccatgatg agcgatcact
    34861 tttgctacac tttctcgatc attcactaat attgtatcaa tacctgctgg tccttctgga
    34921 caattcaatg cgataactgg aatactcatt tttgtgagca tttcaaatcc agaccactct
    34981 tcttcggata agaagacaat cagcccatcg atcacatgct tcatcaggta ttcaattttt
    35041 tgttcaattt tttctggctg atcggcaaac ccgcttaata ataagctata cccattttct
    35101 tcagctactc tttcgatccc tgagacaact tccgacccaa aacggctgga aatcccgttt
    35161 atcaaaagtc caatagagaa gcttcgatta ttcttcaacc ctttagcaat gatgttttct
    35221 ttatagtcca acgctgaaat cgcttctgct atttttttca tgttcttttc cttcagttgc
    35281 tgaccattca agtaacggga aactgttccc actgaaacac cagccatctt agccacatca
    35341 cggatcgatg ccatacattc tcactcctgc cagttacatt tgatttctct ttcttgttgt
    35401 tcgattatga tgaccgcatt ctttgcatag tgttgcttgt catcaatatc catataacta
    35461 aagtcttcag tagagcaact tcctctcact attaattata cttaaacaag tatttgaacc
    35521 ggttcatttt taatatagca tttttttaaa agattgcaac acttttttaa aacgctttct
    35581 gaaagtggcg gattttttgc ctttttaaaa ttttattcta aaatttactc tatttgtggg
    35641 tgttttgtgc gtttttctct tccatccgtt tgaagtttta cattccaatg gtataatttc
    35701 tttatcctat caaatggggg aagtattttg aagaaattaa tcgtactatt tattttgtct
    35761 gcgggacttt tgacattgac tggctgtgat ttgtttggcg gctctgacca atcctcggac
    35821 gaaacaaccg attcttctgt cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat
    35881 acgctaaaag tacgtaaaga tatggattta tccgcgggac agttgacatt tacagataaa
    35941 aaaatggaat ggacacgcac gtatcagaca caaggaactg attcttctga atcatctgaa
    36001 gaaaaaacag tgctgacaga tatcaagatc actaccaaag atgacgaata tattatttca
    36061 ggaaaagaaa ataaaaaaga aacaacagtc atatttacaa aaatcggtaa ttaccgtttg
    36121 aaggatgagg atggcaacat ctacagtcta tagtgccgtc tgttggatct tcttttgttt
    36181 ctccccttct tttctgctat agtaaagaag caagatgatt gtaaaggaga cgaaatggat
    36241 gatcgatagt cctgattatt gtacatgtaa taacaacaaa aatatccata cagaatttaa
    36301 tgaccaagga agcttgagca tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa
    36361 aacatttgaa tcttatcctg ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc
    36421 aagcaaaact cactatggct gatttttatg aacttcaaac tcagcttctt cctttgatca
    36481 agcttcatca gtaaaacaaa cagtggatca tttcagttta caaatcaaag agtaaagatt
    36541 tatttatttc aaatatttga atataaaata accaaacgat agaaaggctt attctacatc
    36601 acgtagtaat ttttctttcc cgtcgtttgg ttttttattt ttagcctaat tcttgacctt
    36661 ttgcatgctg gttatccgta aaatattttc cagcaggctt tccttccaat tcagctaatt
    36721 tttcttggat ctgacggatc accttttttg catcttctgg attttctaca aaatcgaatt
    36781 tatccccatc cacttgtatt ttaggacata catcaaaatc ttcataccat tgattatagc
    36841 gggtgttcaa cgtttcataa taggtatata acgtctcatc gctttctagc tgttcatagt
    36901 cacgcccacg ttttttgata cgttctagca tcgtgtccaa agaaactttg atatggacca
    36961 tcaaatcagg ccgttttttt ggagcaacat catctaactc gtttagcatc gtatctaata
    37021 agctatcgta ctgctgtact tcgatatccg ttacccgacc taaatcagcg tttagatgga
    37081 acaacaggct atcttcatag atggatcggt ctagcacatt gtcatcgttc actaaagcat
    37141 ttttcattgc taaaaatcgc ttgttcaaaa agaagatttg caataaaaaa gcgtactgct
    37201 caggatttga ataaaagagc ggcaatactt cgttatcttc gacattttca taaaatggtt
    37261 tagaatcaat ctcttttgca agcatatccg tcaaactgct cttacctgca ccaatcgttc
    37321 ctgaaatcaa aatcactgac atacaaccct tcctcttcta taagtactcg cctacgtaag
    37381 tttcaacagg caatgtttca aaatcatatt cacgtacagc ttggtctgat tttaaatacg
    37441 tatggagact ttgtgtaatc atcaagttcg taggtaccgt ctcttcttgt acaatgatca
    37501 tcggtttttt caacatagtt gccacaccta attcgtaagc cgttcctgga tcgattgttt
    37561 gatgctcaaa gtcaaggata gcaacaacaa attctgcatt tgtcagttct tccatatctt
    37621 tttcgtatac ttcctgcgcc catccagcgc tgaatagttc ataattgctt tcttggtgat
    37681 gacgtggaga gtagaaactt gtgactgttt tgttttcctc caacgctttt tcgatacgac
    37741 ttactcgatc gatttgctcc tctgaaaaaa atggtcctgc taaatatatt tgactcatta
    37801 ttttgcctcc ttcaataata actgattttt aatagatact gctcgtaatt cattgaccag
    37861 ttccaatgga ttgttggaaa agattacttg atccaaccat tttttatcca taaaatcctc
    37921 ttttgcaaaa gtttctaata attgttgcaa gccgctgtaa taaccgccaa tattcaatat
    37981 tccgattggc ttgtcgggcg tcagaccgat tgctatccaa gaaagaagtt gtgagaattc
    38041 ctccaatgta cctagacctc cagggaaaac gaggacagca tctccctttt caaataataa
    38101 tacctttctt tcatcgatcg tttctgtagg aataaatgta gtaacatctt ttccaggcaa
    38161 ttcagtttca aaaagcccat tcggatagat acctgtgact tttccgttat tttccagtac
    38221 tgttttacta atcactccca taatcccgga gtatgaacca ccgtaaacca gttcgatttc
    38281 ttcttgtgcc atgtattttc ctaacgtttg cgcaataaat ttgtaacttt ctttattacc
    38341 aaaacgggaa ccgcaaaaaa cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc
    38401 tatcttcatt ctacacgtgt ttttttaaaa taaaagacta
//
LOCUS       DAYYNQ010000014        38412 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000014 DAYYNQ010000000
VERSION     DAYYNQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.014"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            510..1433
                     /locus_tag="ACHBJ1_000677"
     CDS             510..1433
                     /locus_tag="ACHBJ1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM9429644.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            1642..2220
                     /gene="pgsA"
                     /locus_tag="ACHBJ1_000678"
     CDS             1642..2220
                     /gene="pgsA"
                     /locus_tag="ACHBJ1_000678"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM9429645.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2460..3701
                     /locus_tag="ACHBJ1_000679"
     CDS             2460..3701
                     /locus_tag="ACHBJ1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM9429646.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3810..4859
                     /gene="recA"
                     /locus_tag="ACHBJ1_000680"
     CDS             3810..4859
                     /gene="recA"
                     /locus_tag="ACHBJ1_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM9429647.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5270..6826
                     /gene="rny"
                     /locus_tag="ACHBJ1_000681"
     CDS             5270..6826
                     /gene="rny"
                     /locus_tag="ACHBJ1_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM9429648.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6905..7711)
                     /gene="proC"
                     /locus_tag="ACHBJ1_000682"
     CDS             complement(6905..7711)
                     /gene="proC"
                     /locus_tag="ACHBJ1_000682"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM9429649.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7852..10425
                     /gene="mprF"
                     /locus_tag="ACHBJ1_000683"
     CDS             7852..10425
                     /gene="mprF"
                     /locus_tag="ACHBJ1_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM9429650.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10579..11430
                     /gene="ispE"
                     /locus_tag="ACHBJ1_000684"
     CDS             10579..11430
                     /gene="ispE"
                     /locus_tag="ACHBJ1_000684"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM9429651.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11675..12613
                     /locus_tag="ACHBJ1_000685"
     CDS             11675..12613
                     /locus_tag="ACHBJ1_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM9429652.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12636..13316
                     /locus_tag="ACHBJ1_000686"
     CDS             12636..13316
                     /locus_tag="ACHBJ1_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9429653.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13289..14113
                     /locus_tag="ACHBJ1_000687"
     CDS             13289..14113
                     /locus_tag="ACHBJ1_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM9429654.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14280..15098
                     /gene="purR"
                     /locus_tag="ACHBJ1_000688"
     CDS             14280..15098
                     /gene="purR"
                     /locus_tag="ACHBJ1_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM9429655.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15601..16974
                     /gene="glmU"
                     /locus_tag="ACHBJ1_000689"
     CDS             15601..16974
                     /gene="glmU"
                     /locus_tag="ACHBJ1_000689"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM9429656.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            17068..18039
                     /locus_tag="ACHBJ1_000690"
     CDS             17068..18039
                     /locus_tag="ACHBJ1_000690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9429657.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      18045..18147
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18213..18782
                     /gene="thiT"
                     /locus_tag="ACHBJ1_000691"
     CDS             18213..18782
                     /gene="thiT"
                     /locus_tag="ACHBJ1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM9429658.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18863..19636)
                     /locus_tag="ACHBJ1_000692"
     CDS             complement(18863..19636)
                     /locus_tag="ACHBJ1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429659.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19903..21603
                     /locus_tag="ACHBJ1_000693"
     CDS             19903..21603
                     /locus_tag="ACHBJ1_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM9429660.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21573..21791
                     /locus_tag="ACHBJ1_000694"
     CDS             21573..21791
                     /locus_tag="ACHBJ1_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM9429661.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21811..22701
                     /locus_tag="ACHBJ1_000695"
     CDS             21811..22701
                     /locus_tag="ACHBJ1_000695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM9429662.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22719..23003
                     /locus_tag="ACHBJ1_000696"
     CDS             22719..23003
                     /locus_tag="ACHBJ1_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429663.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23490..24104
                     /gene="gmk"
                     /locus_tag="ACHBJ1_000697"
     CDS             23490..24104
                     /gene="gmk"
                     /locus_tag="ACHBJ1_000697"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM9429664.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            24109..24414
                     /gene="rpoZ"
                     /locus_tag="ACHBJ1_000698"
     CDS             24109..24414
                     /gene="rpoZ"
                     /locus_tag="ACHBJ1_000698"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9429665.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24667..27075
                     /gene="priA"
                     /locus_tag="ACHBJ1_000699"
     CDS             24667..27075
                     /gene="priA"
                     /locus_tag="ACHBJ1_000699"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM9429666.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            27090..27581
                     /gene="def"
                     /locus_tag="ACHBJ1_000700"
     CDS             27090..27581
                     /gene="def"
                     /locus_tag="ACHBJ1_000700"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9429667.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27574..28512
                     /gene="fmt"
                     /locus_tag="ACHBJ1_000701"
     CDS             27574..28512
                     /gene="fmt"
                     /locus_tag="ACHBJ1_000701"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM9429668.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28512..29870
                     /gene="rsmB"
                     /locus_tag="ACHBJ1_000702"
     CDS             28512..29870
                     /gene="rsmB"
                     /locus_tag="ACHBJ1_000702"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM9429669.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29883..30623
                     /locus_tag="ACHBJ1_000703"
     CDS             29883..30623
                     /locus_tag="ACHBJ1_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM9429670.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30620..32689
                     /gene="pknB"
                     /locus_tag="ACHBJ1_000704"
     CDS             30620..32689
                     /gene="pknB"
                     /locus_tag="ACHBJ1_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM9429671.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32854..33753
                     /gene="rsgA"
                     /locus_tag="ACHBJ1_000705"
     CDS             32854..33753
                     /gene="rsgA"
                     /locus_tag="ACHBJ1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM9429672.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33766..34416
                     /gene="rpe"
                     /locus_tag="ACHBJ1_000706"
     CDS             33766..34416
                     /gene="rpe"
                     /locus_tag="ACHBJ1_000706"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM9429673.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34417..35055
                     /locus_tag="ACHBJ1_000707"
     CDS             34417..35055
                     /locus_tag="ACHBJ1_000707"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM9429674.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35210..36064
                     /gene="mreC"
                     /locus_tag="ACHBJ1_000708"
     CDS             35210..36064
                     /gene="mreC"
                     /locus_tag="ACHBJ1_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM9429675.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            36068..36577
                     /gene="mreD"
                     /locus_tag="ACHBJ1_000709"
     CDS             36068..36577
                     /gene="mreD"
                     /locus_tag="ACHBJ1_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM9429676.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36832..38406
                     /locus_tag="ACHBJ1_000710"
     CDS             36832..38406
                     /locus_tag="ACHBJ1_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM9429677.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT        12633 a         6873 c         8356 g        10550 t
ORIGIN      
        1 aattgcttct caaatttttg tgatatttcg gcttattgcc gaagaagcta cttctggaac
       61 accgtgtacc aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
      121 aaaaacagct tcttaatgtt tttaacttgg ttggacttat taccgcagat gcaagctttt
      181 gaacaccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
      241 tacggaaaaa tatctcctta tattttgccg gtttttgagc taataccgag gaacagctga
      301 ttgaacaccg tttaagaaag aggtcgtgag cctgccgttt aactccgagt cacaaggaac
      361 aagttcaaaa aacagcttct tatatttatt ggatattgtg acttgtgacc gaggagttgg
      421 caggagaaca atgtttattc ggaaaaaagg agctgtgaca tgacttttgt cacagcaccc
      481 aaacataacg tctttcgttg caacagcaga tgcttcggag aatcaaacag actggagaag
      541 atgaacgagt ggcttttgaa accatcggag aaaaattacg gcaagcacgc ctgaataaaa
      601 aaatatcttt agatgaatta cagcagatta caaaaattca aaaacgttat ttggaagcaa
      661 tcgacaatga tgattttgac caattgcctg gtaaatttta cgtgcgggca tttattcggc
      721 aatacgctga agctgtagga gaagatggag accatttagt cgatgttttt gatgggaaaa
      781 gcaaattgac aggatcggtc cctgaacgtc cgcagcctga aacagtaagt acttctagaa
      841 aagcaatgca tcaggaagaa tcgcatccaa gtcgcttttg gacaagtctt cctgtcattt
      901 tattaggatt ggttgctttg gcgattgtcg ttgtcgtggg ctatatgact tggcaagatc
      961 gtcaagctga tccaataatc ggagacacag cttcttcgct taaagtagaa ggatcagtcg
     1021 aggagtcctc ttcctcagag caaagcacgc agaccaaaga gtcttctaca gtagaatcaa
     1081 caacaaaatc aagtgaagaa aagaaaatgg caatagctac aggagaagat tctggcagtg
     1141 ccatcgcagt aaatatcaca gacgctacca agcctgtaac ccttgaattt actgctgtca
     1201 atcgtgtatg gttaggtgtg ctagtagaca atgcctatgt ttatcaaggg acactggcag
     1261 caaatgaaac acagagtacc gtgttaccgg aaacagcaac aaatgcgacc atcacgattg
     1321 gtgcagcaag caatgcaacg atcaaagcga atggagaatc cgtaccagtt aatccagggg
     1381 aaaacaatca gagtccaaaa aatgtgaacc ttactcttca atacgcagaa tgatcgtata
     1441 aattagttta cgcatctgtt tacttgtagg tataaaaaag aaggatcgtg acaaatctgt
     1501 gtcgcggtcc ttcttcgtta attcaaaaag atcaatttca ataaaaatac tcctttgaat
     1561 aaattcattt ggaaaagtaa ataatatcgg tggaaacttc gctgatagtc acgtagaata
     1621 gggaagaaag gagtcgatag tgtgaattta ccaaataaat taactgtact tagaatttgt
     1681 atgatcccga ttttcatttt ggttgtaggt ttacctttag attggggaca atttaacgta
     1741 ggagacacca cactggctgt cacacagcta gtaggtgcag ttatttttgc tttagctagt
     1801 atcacagact ggttagatgg aaaaattgcc cgtgcgcgag gccttgtcac taattttggc
     1861 aagtttgcgg acccgttggc agataaaatg ctagttatga ctgcttttat catgttggtt
     1921 gaccaaggaa aggcccctgg ttgggtagta gcgatcattg tctgtcgtga attagccgtc
     1981 acaggcttgc gtttactact tgtcgaacat ggagaagtca tggctgcagc ttggccggga
     2041 aaagtcaaga cggctacaca aatggttgct attatcttgc tgtttatcaa taatatccca
     2101 ttcagcctga tcggtattcc agtagatcaa atcatgcttt atgcttgtct gatctttact
     2161 atctattcag gtgtggatta cttcatcaaa aacagcggtg tgttcaaagg atcaatgtaa
     2221 gtatcgttta gtggagaggt tgtgagcctg ccgtctaact ctgagtcaca agggataatt
     2281 tcaaaaaaca gcttttcatg ttttatgtat attgtgactt atgaccgagg ttttggcagg
     2341 agaacactgt ttatttggaa caaggagctg tgacaagatt tttgtcacag ctccttgccc
     2401 tctttttaaa taaaaaaata taaaaataca gcgtatacta tataagggag cgggaagtaa
     2461 tgaaagcaga aataatagct gtcggaacag aacttttact aggacaagtc gtgaatacga
     2521 atgccacttt cttgtcagaa cagctagctg atctaggtat cgaagtttat tatcaaacag
     2581 ttgtaggaga taatcagcag cgtttagaag agttgattgc tctagctgaa acaagaagtg
     2641 aactcatact cttatgtggc ggattaggac cgacagaaga tgacctgaca aaagaagcaa
     2701 ctgcggctca cctcggtaag tctctcatac aaaatacgga aggatacaaa aaattattgg
     2761 catattttga aacaacccat cgaaaaatga ccaaaaacaa tctgcaacag tcccagatta
     2821 tcgaaggcgg ggttcctcta ccaaatcgta cagggcttgc tttagggact ttttatcaaa
     2881 cagatacaca tgcgtatatc cttcttcccg gtccgcctaa tgagttgaaa ccaatgtttg
     2941 tggaacaagt acgtccttta ctagaggaac gttttccttc tgaagaaaaa ctaatttcta
     3001 aagttttgcg cttttatggg attggtgaat cgcgactggt cactgagcta aaagatctga
     3061 ttgagacaca gatcaatcca acgatcgcac catatgccaa accaaatgaa gtgacattac
     3121 gactgacagt gaaaacaaat gatgttcagg cagggaatca agctctttta gcattagaag
     3181 aaaaaatcca agaacgagta ggggaatatt tttatggata cggcgatgat aattcgctag
     3241 caaaagtcgt agtggaacta ctgaaagaaa acaagcagac tgtaacagca gcggaaagtt
     3301 tgacagccgg cgccttccaa gccgcattag gcgacattgc tggtgtatct gaagtatttc
     3361 caggcggatt cgttacctac tcgctccaaa caaaagctgg ttttctggag attgatccgg
     3421 agctattagc agaatatgga acagtcagca aagaatgtgt ggagcagatg gccattcaag
     3481 cacgtgagat agcaagaaca gattacagtg tagctttcac cggagtggct ggaccggaca
     3541 cgttagaagg tcaatctgca ggaacagtat ggatcgctat ttcatctaaa gacggcgtgg
     3601 tttcggaaaa atatcacttt gccagagatc gtgctgctat cagatataat gcggttatgc
     3661 aaggatttga tcttttgaga aaacagcttt taagaaaatg ataaatgcga atatttgttc
     3721 ggttttttct tgcttttttt cactaaaaaa gatatgataa ggataatgaa atgttgctct
     3781 gatttgtaaa tataaggagg attatcgtat tggcagatga tcgtaaagca gccttagatg
     3841 ctgcactgaa aaaaattgaa aagagctatg gtaaaggctc tattatgaaa ttgggcgaaa
     3901 aaatcgacca acaaatctct acaattccaa gtggctctct tgcgttagat gtcgctttag
     3961 gtgtcggcgg ttacccccgt ggacgtatca tcgaagtata tggacctgaa agttcaggta
     4021 aaacaacagt tgcactacac gctattgcag aagtacaaaa aaatggcgga acggccgctt
     4081 tcattgatgc tgagcatgcg ttagatccgc aatatgcaca aaaattaggt gtgaatatcg
     4141 atgaactact tctttcacag cctgacacag gagaacaagg tctagagatc gctgatgctt
     4201 tagtatcaag tggggctgta gatatcgtag tagtcgattc agttgctgct ttagttccac
     4261 gagcagaaat cgacggcgaa atgggtgact cacatgtcgg gttacaagca cgtttgatgt
     4321 ctcaagcatt gcgtaaactc tctggttcga tcaacaaaac aaaaacaatc gctattttca
     4381 tcaaccaaat ccgtgaaaaa gtaggcgtga tgtttgggaa tccagaaatc actcctggag
     4441 gacgcgcatt gaaattctac gcaacgatcc gtttggaagt acgtcgtgca gaacaattga
     4501 aacaaggtac agatattgtt ggtaaccgta caaagatcaa agtcgtaaaa aacaaagtag
     4561 cacctccttt caaaatcgct gaagtagatg tcatgtatgg tttagggatc tcacaagaag
     4621 gagaacttct agacatggca gtagaaaaag acattgttga taaaagcggt gcttggtatt
     4681 cttataaaga agatcggatt ggtcaaggac gggaaaacgc taaaatctat atggctaatc
     4741 atcctgaaat gatggcagaa gtttctgctc tagttagagc agcttatggt attggtgaag
     4801 aagtcgctgt cccagaagat gaaaaaggac aagaagaatt gcctttagta gaagaataaa
     4861 aattatgata aacaaaaacg gctggtctga agagtaggta ctctccttga cctgccgttt
     4921 ttttattgaa taaagaaatc gtgagttatt taattatacg agtgggtaca taaaataaaa
     4981 atatctgttc atctacaaag aataatttaa tagaaaagtt tctcaattta aaggtttacc
     5041 tatatgaaac tagtgtcatt tattttaaaa accatcgttt aatgaataag tagaaagccg
     5101 tttctttgtt tatttaaagt tgacactacg gggtacaaac attagaataa agttgtgcat
     5161 gttttttgta cacatatatg cgaagatgca tattgacgag tttagaataa tttatcatag
     5221 aagctgtata gatgataaaa atgaaaaaga tagtatggag gtggatttta tggtattcaa
     5281 cattctcctc gctatcatcg gtttaattgt cggtcttggt ttagggttta tggtcgcaaa
     5341 gtcacgtcac gagaaagaaa tagctggtgc acaatcatca gctgctggaa tcatcgatag
     5401 tgcaaaaaaa gaagcagaaa ctctaaaaaa agaagcattg ttagaagcta aggaagaaaa
     5461 tcagaaatac cgttctgaag ttgaaagtga gctgagagaa agtaagttag aattaaaatc
     5521 tcaagagaat cgtttgattc aacgcgaaca aacgctgaat cgtaaagatg actcgttaga
     5581 aaaacgcgag aactcacttg aaagtaaaga agaagcactt agttcaaaac agcaattaat
     5641 tgaagagcga gaaaaagacg ttgaaaaaat gattgaagat caacaaaaag aactagaaaa
     5701 aattgctggt ttatcaaaag aggacgcacg agatgtcatc atgaaatcga ctgaagaaga
     5761 gctgaatcat gagttaactc taatggtaaa agaatctgaa cagcgtgcca aagaagaagc
     5821 agatcgcaaa gcgaaaaact tattatcatt agcgattcaa cgctgcgcag cagatcaagt
     5881 ttcagaaacg actgtttcag tcgttaccct tccgaatgat gaaatgaaag gtcgtatcat
     5941 tggacgagaa ggtcgtaata ttcgaacgct tgagacgctg actggtatcg atttaatcat
     6001 cgatgataca ccggaagcag ttgttctttc cggattcgat ccaatccgaa gagaaatcgc
     6061 acggatgacc ttggaaaaac tgatacaaga tggacgaatc catccagcgc gtatcgaaga
     6121 aatggtcgag aaatctcgta aagaaatgga tgaacgtatt cgcgaatacg gggaagaggc
     6181 agcgtttgaa gtcggtgctc atacacttca tccagatcta atcaagatca tcggacgtct
     6241 gcgattcaga actagttatg gacaaaacgt cttgaagcat tcggtcgagg ttgcaaaatt
     6301 agctggtatc ttagcagcag agcttggcga agatatccaa ttagcaaaac gtgccgggtt
     6361 gctccatgat attgggaaag cacttgacca tgagattgaa ggatctcacg ttgagatcgg
     6421 tgcggaatta gctgccaagt acaaagaaaa cccagtagta atcaacgcga tcgcttctca
     6481 tcatggagat gtggaagcta cttctgttat ctctgtcctt gtagctgcag cagatgcgct
     6541 ctcagcagca agacctggtg cgcgaagtga atcattagaa aactacatcc gtcgtttaag
     6601 aagtctggaa aatatttcga acagcttcgc cggtgtagaa tcaagttacg cagttcaagc
     6661 tggacgcgaa gttcgggtaa tggtcaagcc agaagaaatc tcggatttag acgctgttcg
     6721 tttagtacgt gatattcgta aacgaatcga agaagaactg gattatcctg gacacatcaa
     6781 agtaatcgtt attcgggaaa ctcgtgcaac tgattatgct aaataataaa aaatacaaaa
     6841 gaagagccgc ggcaaaagcc acagctcttc tttttttgta aggcacgaaa aaacattgct
     6901 atcattattt tttattcaac atctcaagat ctttctcgat cgtcgtattg attcctttga
     6961 tgattgctgg gatgaatcct gcttcttcta aggcaactac acctgcaaca gttgtaccgc
     7021 caggagaaga aacttggtct acaagttccc atggattttt atcacttgaa aggaattttt
     7081 gacaagaacc cataattgct tttgcagcaa tctcagtggc tgtctgtttg gacaaaccat
     7141 tcagtacacc agtccgtccc atcgcatcga tcagcatgta aacgaatgcg ggtgagctgc
     7201 cagcaaggcc aatgaatgta ctgaaatctg attctgctaa aggatagacg ctgccgattg
     7261 cttcaaaaat catcgtagct tgctgaagct gttcctccgt ggcagcttca tttttgcaaa
     7321 tcgcagaaac gcctgctcct acgctgacat tgacattcgg catcacacgt acgatggagg
     7381 cctgaggatc ttcaagcgct gtttctaaat ctattaatga ttttcctgaa gcaatagaaa
     7441 tgaataagac tttctggcta tattctctta gttcttccaa tactttttca atgatttttg
     7501 gtttcacggc taaaagaacg atatctgatt gtgatgcaac ttcctgatta cttttgcatg
     7561 catggacgcc agtttcagca gcaaattttt gtgcattttc aaaatgtcca ctggaaacga
     7621 acacctcttc tttcgagact gttccttttt ttaataaacc atcaataata gcttgtgcca
     7681 tattccctgt gccgataaat cctactttca tgttatcctc tcctttagca ttaggatatc
     7741 tttctttttc tatcatcgca agcaaagaac aggtctaaag tggaaaaatt aaaataaata
     7801 agataacatt ggggtatgac acaaaagtat acaagttagg aatgggaaaa tgtgaaaaat
     7861 aaacgatact tgatgctgca atggataaag gaacatagtc tgattttaaa actgattttt
     7921 ttcggctccg tgttagtctt tgtggcaaat caagtcgcaa atattgctaa tggcatgagt
     7981 tggcaagata tttggcaaag gatgggacaa caaagccgca tgacgatcct gctcatgatt
     8041 ctggctggac tgctgggtgt cactccgatg ttgttgtatg attgggtgac gatcagagtc
     8101 ttagaaaaac aaggaaaacc aaaaatggaa agaaaagagt ttcttcttgc tgcgtgggta
     8161 acaaacacga tcaacaatct cgccggtttt ggtggtgtgg taggagcaag tctgcgggct
     8221 agtttttatg gaaagcagac aaatagaaaa atggttttag ccactgtatc aaaagtcgca
     8281 ctatttatgc tgactggttt gtctgtatgg tcgtttctta catttattga tgtctttttt
     8341 attcagtcag aaagtatttt tcgaagctat tggatctggc ttttcggcgg tagtttttta
     8401 gcaccagtca ttttcttttt tgcttatcta aagagaaaga cattgttctt cgagttttat
     8461 ccaaaaggct tgcttggtct gctactggca tcctttggac aatggacggg agcccttgcg
     8521 gtatttttga tcatcggcaa gttgatgaga atagatattt cgatgctttc gatctatcct
     8581 atgttcatga ttgctacatt gataggaatg ctgacgatgg tcccaggtgg aatggggact
     8641 tttgacgttt tgatgatctt aggaatgagt caattgtctg ttcggcaaga tgtagcagta
     8701 gtttggctat tatattatcg gttgttttat tatgttctac cttttatgac gggacttcta
     8761 ttgttcatca cacatacagg agtcaaaatc aatcgatttt tagataacat tccgcgactg
     8821 tttttccaaa aggttgcgca tatcgtgctc gtaagtgctg tctattttgc ggggatcatg
     8881 atggtacttc tatcaacaat cactaatctg tctgcaatca gtcggctatt tcaagtatta
     8941 ttgccgttct cattcaattt tttagatcag acagtgaatc tattggttgg ctttttatta
     9001 ctcggccttg ctagagcact ttcagtaaaa gtcaaaaagg catttctgcc tacgattgct
     9061 ttactatctt ttggtatttt aaatacaatc acacgtacgt tatcttggca gttgatattg
     9121 gtatatgtct tgattctgtt agctgtgtgg ttatccagaa aagaatttta tcgaacaaaa
     9181 ttcgtttatt cttggggagc tataatcttt gatgctagct tgttcggctt tttgtttatc
     9241 gtttacgcaa ttgcaggcta tcacagcgga cgctttttag tcggtcgaat aacaggaaat
     9301 cattttattt tgtttccttc agatgatgtg tggttctcag ggcttattgg tttgggcatc
     9361 tcattgctag ctttagtggc gctttatcaa tatttagctg aagatactac gaagcttggc
     9421 agtgagtacg aaaaagaccg ttttgaccgt ttattggaaa catacggagg cacagctaca
     9481 agccatcagc ttcgcttgtc aggttacagt tatttttatt accaagaaga cgaccaagat
     9541 caagtcgttt ttggttatca gatcaaagga aacaaatgct ttgttttagg agatccagtt
     9601 ggcaatccta aaatgatgcg ggcagcaacg aatcagttta tgaaacaagc agattgttta
     9661 ggctatcagc ttgcgtttta taagatatca gaaagctacg tagtgatgct tcatgaattg
     9721 ggttttcact tcacgaaagt aggtgaagca gggattgttg atctagcaac agaagacttg
     9781 tctcggctga gtcagcaact tgaatatcaa cgtttgatga aagaaggata ccattttact
     9841 tggtacgatc aactgccgga agaattacat caagctgtaa atgaagtttc acaaaaatgg
     9901 cttcaaggtg cacgagaaaa atacttctct gtgggacgat ttgataaatc atatgtatta
     9961 tctagcggtg tcggtattgt ccgtaaagat caaaaagtcg ttggcttcat caccgctcag
    10021 ccgatgacga agaagcaggc gggatatgat ttgttgcggg tcttacctaa agaatctgag
    10081 gaattagctg actatctgtt agccaatctg tttattgtat acgagaaaat gggctactcg
    10141 gaagccaatc taggcttagc tccattagct aatgttggag aaacagattt ttctttcttt
    10201 caagaaaaag tcatgcatat cgtatataat tatggtaatt ttttctattc cttccagtct
    10261 gcttatgaaa agaaattaaa atatgtcgat cattgggaag gacgttattt tgcttatagg
    10321 aaaggaagta atttcggatt tgctaccatg cagctactca gtctgatagg aaaagaaaaa
    10381 ggaaatgttc cgagtttggc agaagaagtg ctcacagaat tataagaatc actagaactt
    10441 gtttggatta tgaagagaaa attctgtgaa agctgttgaa acgatgacaa aatcaaccac
    10501 taggcagaca taagctatac ttttattttt ccctatgata gaatacttgc gacttagact
    10561 tttcgaatgg gtgagaggat ggaattgatt gaaaaagcgc cagccaagat aaatttagga
    10621 ctggatgtat tatatagacg gcaagatggg tatcatgagt tggaaatggt gatgtcgagc
    10681 gtagacttgg cagatcatct attttttgaa gaaataacag aagacaaaat cattgtagaa
    10741 acaaataaag catttttacc ggtagatgaa cggaacaatg tttatcaagc tgctttattg
    10801 ctaaaaaata aatacaatat caaaaccggt gtcaagatct ttgttaagaa gaatattcca
    10861 gtagcagctg gattaggcgg aggaagtaca gactgtgcag ctgctctaag agggatcaac
    10921 caattatgga atctgggctg ttcgttagaa gaactggcag aaatcggttt ggaagtcggt
    10981 acagatgttc cttactgtgt ctacggaagt acggcatttg tcggtggcaa aggggaagaa
    11041 atccaagtat taccttctat gccacagtgc tgggttgtgt tggtcaaacc aagaatgagt
    11101 gtctctacac gtacagtgtt tagagaagta gatacgcaag ccatccaaca tcaagacatc
    11161 cacggacttg ctgaagccat ccgagagcaa gattatcagc agatgatcaa aaaaatggga
    11221 aacagtttag aagatataac gggaaagcgg catccagtca tccatcaaat caaagagcgt
    11281 atgatcaagt acggagcaga tgctgcattg atgagtggaa gcggaccaac cgtttttgcc
    11341 ttgtgtcaac attattcaag agcgcagaga gtatataatg ggttaaaagg tttttgtgag
    11401 gaagtttatt tagtacgaac cctgcaataa aatataagaa gtaaaatagg tgatgaaaaa
    11461 ctgaggaatc ctaaaatggt attcttcagt tttttattgt agtgaaatgg ctagatatga
    11521 gcagcaactc atccatttta cgatttaatc aagaatatac atgcttaaat cagaaattga
    11581 tggatttcta ttgactgtgt ttttttattt tgatacatta gttcacgtta ataaatcgta
    11641 aaattccgat ttgttcattg aaaggagcat ataaatgaaa aaatatcttt tgacagctac
    11701 ggtaatgata ggtgctttac tgtttgcagc atgtggaaat acgaacaagg aagcagacaa
    11761 aaaagaggat ttgacgatcg ttacgacttt ttatccgatg tatgatttta caaaagagat
    11821 cgtaggggat gaaggaaacg tgaaactttt gatccctgct ggaacggagc cgcatgattt
    11881 cgaaccaagt gcaaaagaaa gagcagaaat ttccgatgca gatgttttcg tgtataatag
    11941 ttcagacatg gaattttttg tggattcgtt aaaggattca gtggatagca aacagacctt
    12001 gatgatcgaa gcagctaaag gaatcgaccg tttagaatcg caagaagcag acgaacatga
    12061 agaatcagaa gaagggcatg gtcattcaca tgaatacgat ccacatgttt ggctcgatcc
    12121 ggtattagct ataaaagaag tcagaacgat tgctgaagaa ttaggcgaaa aatatccaga
    12181 taaaaaggaa acttttacga agaatgccga tgcttatatc aaaaagcttg aagcactgga
    12241 tcaaaaatac tccgatgagc taaaagatgc aacaaaccgc acatttgtca ctcagcatgc
    12301 tgcttttgct tatttagcga atcaatacga cttaaaacaa gtagcgattt cgggcgtttc
    12361 accagatcag gaacccacac caagtcgctt agctgagcta aaagaatttg tgaagaaaaa
    12421 caacatcaaa gtgatttatt ttgaagaaaa tgcctcttcg aaggtagctg agacattgtc
    12481 gaatgaaaca ggagtgaagc tagaagtgtt gaatccttta gaaagcttga ctaatgaaca
    12541 aatcaaagca ggcgaaaatt atatttctgt catggaaaaa aatctgaaag ctttaaaaga
    12601 aagtatcaat taagttttag aatggaggtt gtaaggtgcg ttatatcgaa atatctgatt
    12661 tgacctttta ctatgatgat gaacctgtac tagaagatgt ctcttatcat gtagatgcgg
    12721 gtgaatttgt cattttaact ggagaaaatg gagcagccaa gtcaaccttg gtgaaagcat
    12781 cgttaggatt actgaaacct tcaaaaggaa tgataaagat tgctgagaaa aatcaagaag
    12841 gaaaaaaatt aagtatcggt tatattcctc aacaagttgc atctttcaat gcaggttttc
    12901 ctagtacggt gatcgaatta gtccgttcag gaagatatcc aagaggccgc tggttcaagc
    12961 gtctctctga aaaagatcat caacatgtac agcgtgcttt agaagctgta gggatgtggg
    13021 atatgcgaaa tcggcgaata ggtgaattgt ctggcggaca aaagcagcga atcagtttag
    13081 cgcgtatttt tgctactgac cctgacttat ttgttcttga tgaaccaaca acaggtatgg
    13141 atgaagaatc cagaaatgaa ttttatcgtc ttctccgaca tagtgcgcat gtccatggga
    13201 aatcagttct aatgatcacc catgatcatg aagatatcaa acagtatgcg gacagacaga
    13261 tccgactggt gagaaaggag gattctcaat ggcgttgctt tcatatgaat tcatgagaag
    13321 agcgtttctt gccgctatct tcattgccgg aatcgccccg atgctaggtg tgtttttagt
    13381 gatcagaaga caatcgctga tggctgatac gctttctcat gtctctttag caggggtagc
    13441 gttaggattc tttttgaacc ttaatcctac acttacgacc atgctagtcg ttgtattagc
    13501 agctgtgata ttggagtatc tgcgaacgat ctaccgaacc tattcagaaa tctccattgc
    13561 cattttaatg gctggtggtt tggcgctagc gttagtattg atgaatttga gcggagggaa
    13621 ttcagcaaca agtatccaat cctatctgtt tggttcgatc gtgaccatta catgggagca
    13681 agtcatcttt ttaggaatct tattcgttgc catcggtata ttgttctttt tattcaaacg
    13741 gccgatgtat gttctgactt ttgatgaaga taccgcccac gtggatgggc tacccgttca
    13801 tttgatgtcg atgatgttca atgtgattac tggtgtggct attgctgtga tgatcccgat
    13861 tgccggtgca cttttgatat ccgcaatcat ggttcttccc gcagcaatcg gtatgcgatt
    13921 aggcaaaagc ttcaatgtcg tgattgctat cagtgtttgt gtaggatttc tagggatgct
    13981 gtcaggacta actagctcgt tttatctaga tacacctcca ggagcaacaa tcacattagt
    14041 ctttattgct ttgttcctga ttgtaaatat catcaaacga atattggttg ccttcagaag
    14101 aaaaaataac taaattataa agaaaagaag ccttataaaa acgaaaaatt ttttataggc
    14161 ttctttttat tttaataaat accgaactat tttcttattt tttcataaaa tgctcgaatt
    14221 tttgaagaaa aacttatata atgtaaaacg ataaaatttc ttaaaaggag ttggaatagg
    14281 tgaaagtacg tcgcagtgaa cgtctagttg acatgacaca gtatttattg aatcacccgc
    14341 atgaattgat ttccttaaca agctttgccg aacgttatga atctgctaaa tcttctatca
    14401 gtgaagattt gggaatcatc aagaaaacat ttaaagaacg agggtatgga accttggaaa
    14461 caatcccggg tgctgcaggt ggtgtattgt tcatccctga aatttcatat gaagaggcga
    14521 aaaaatatat tgaatctcta gcagaacgtc tatcagaaca agaccgtctg cttccaggag
    14581 gatatgttta tctctctgat ttgttaggag aacctgaact gctgcgtcaa gttggaagaa
    14641 tcattgcttc taaatacctt ggtgaaaaaa tcgatgctgt aatgactgta gccacaaaag
    14701 gcgtacctat tgcccaagct gtgtcctatt atctgaatgc gccattcgtt attgtacgcc
    14761 gggactcaaa aataacagaa ggatcaacag taagtgtcaa ctatgtgtct ggatcttctg
    14821 aaagaattga aaagatggaa ctgtctaaga gaagtttgaa acgtggttca cgtgtccttg
    14881 tagtggatga tttcatgaaa ggcggcggaa cagtcaacgg aatgaagagc ttgattgaag
    14941 aattcgaatc agaacttgtt ggcatcactg tatttgccga atcaaaattc aacggtcatc
    15001 gcgccatcga tgattatacg tcactgcttt atgtcaaaga tgtagatact caaacgaaaa
    15061 atattacggt tgtgccagga aattattttt cagaataaga ggttgtgaaa aagccgctta
    15121 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acattttttc ataaatttgg
    15181 cttaataccg agaaactggc ttttgaacac cgtttatcta gaggttgtga aatcagcgtc
    15241 ctgtcctgag tattagctca ataaacggaa aaattgctcc tcatattttg ccgtttattg
    15301 agctaatacc gaaggtcagc tgattgaaaa acgtttaatg aagaggttgt aagcctaccg
    15361 tttaactcta agtcacaaga aacgatttca aaaaacagct tttcatgttt tatgagtagt
    15421 gtgacttgtg accgaggagt tggcaggaga gcaccggtta ccaaatagat aggcccgtta
    15481 tggtctatct atttttttat ccagaaaacg ctgggataaa agaaaatcac ttgttttgtt
    15541 gaaaaaatgg gtatactggg ataaactgaa atcagcaaac tatatttagg agtgacgcag
    15601 ttggacgcac gttatgcaat tattttggcg gcagggaaag gtacccgcat gaagtcaaaa
    15661 ttatataaag ttttacatcc cgtttctggt aaacctatgg tagaacatat cattaatcgg
    15721 gtcagtgaaa cgaaaccaga tgaagtgatt acgatcgttg gacacggagc agaacaagtt
    15781 aaagcacaac taggagaacg cagtaaatat gcgttacaag cagaacaatt gggaacagga
    15841 catgctgttt tgcaagctgc ttctttcttg gaaggaaaaa aaggaacaac gttagttatc
    15901 agtggcgata caccattatt gactactgaa acgctgaaca atctttttga atatcaccaa
    15961 ggaaaaaatg caagtgcaac gatcttgacg gcacaagcag aaaacccaac aggatatgga
    16021 cgaatcatcc gtgaccatat tgggatcgta gaaaaaatcg tggaacaaaa agatgcaaca
    16081 ccagaagaag ctcttgtaca agaaatcaat acaggaactt actgttttga caacgaagct
    16141 ttgttcgatg cgttaagcaa agttggaaca aacaatgctc aaggtgaata ctatctaaca
    16201 gatatcatcg aaatcttaaa agaagaaggc catacagttg ctgcttatca aacggatgat
    16261 tttgaagaat caatgggagt caatgatcgg atcgcgttag ctaaagcaaa tgagatcatg
    16321 agaaagcgaa ttaaccagat gcatatggtc aatggcgtaa gctttgtcga ttcagcaacg
    16381 acatacatcg atgcaggcgt agaaatcggt ccagacacat tgatcgaagc aggtgtacaa
    16441 atccaaggaa atacagtcat tggttctgat tgtgtcatcg ggtcacattc aaaaatcgta
    16501 gacagtagga ttgaggatca cgtcgtcatt gaaaactcag tcattgaaag cagccatgtc
    16561 aagaaacatg cagatgtggg accttatgca catttacgac caaaagctga aattggcgaa
    16621 aacgtccata tcggaaactt tgtggaagtg aaaaatgctc aaatcggcaa aggaactaaa
    16681 gtcggtcact tgacttatgt tggtgatgct acgctgggag aagaaatcaa tgtcggatgt
    16741 ggtgttgtct tcgtcaacta tgatggaaag aacaaacatc atacgactgt cggcgaccac
    16801 agctttattg gttctagcac gaatatcatc ggacctgtcg aggtagctaa aaacagttcg
    16861 attgctgccg ggtcaacaat tacagacaat attccagaat atgcgctggc aattgcgaga
    16921 gcaagacaag tcaataaaga aggatatgca aaaaaactcc cttatctaaa ctaagacttt
    16981 tgcaaaatca cttgatttat ttaaaaaata tgactactat ttattaggta gaattctaga
    17041 gttgtgaaaa agaaagtgga ggtccccatg tcaaaacatt actttgaccc aagattaaaa
    17101 atttttgcgt taaattccaa tcgcccatta gcagagaaaa ttgcagcagc agtcggcgtc
    17161 gagttaggaa aatcatctgt tactcaattt agtgatggag aaattcaagt aaacattgaa
    17221 gaaagtattc gtggatcgca tgtctatgtt attcaatcaa cgagcagccc agtcaatgat
    17281 aacttgatgg agttgctgat catgattgac gcactgaaac gagcaagtgc aaaaacaatc
    17341 aacgtagtca tgccttatta tggctatgcg cgtcaagacc gtaaagcacg tgcacgggaa
    17401 ccaatcacag cgaaattagt agcaaacatg attcaaaaag ctggtgcaac aagaatgctg
    17461 actttggacc ttcatgcagt ccaaatccaa ggtttctttg atattccagt cgatcatttg
    17521 atgggtgcgc cacttattgc caattatttc ttagaaaaag gaatcaaagg cgatgatgtt
    17581 gtcgttgttt caccagacca tggtggtgtc acacgggcac gtaaattagc ggaatttttg
    17641 aaatctccga ttgcgatcat agacaaacgc cgtccaaaag caaatgttgc agaagtaatg
    17701 aatatcattg gacaagtcaa aggcaaaact tgtgtactga tcgatgatat gatcgataca
    17761 gcaggtacga tcacattggc tgccaatgct ttgaaagaag ctggtgcaac tagtgtgtat
    17821 gcttcatgta cacatccagt attatccggc cctgctttac aaagaataga agattcagct
    17881 atcgagcgtc tagttgttac agactcgatc tatcttccag aagatcgtaa aatcgacaaa
    17941 atcgatgaag taagtgttgg cggactaatg ggagatgcaa tcaaacgtat ccatgaaaac
    18001 aaaccagtta gtccattgtt tgaaacaaaa aataaataat aaataaactc acaaagggga
    18061 gtccaattgg gctgagattg aatctatttc taaacccttc gtacctgtat cggttatgcg
    18121 agcgtaggaa ttgtgaataa acagctgttt gactattttc gaaagctgct tcctcctttg
    18181 tgactttttt attcaaagga ggaagttttt ttatgggaaa aaatagagtt tgggtagaag
    18241 gaacgattgt tgcagcgtta gcgatggtgt tgtcgttgat tccgatccag atcggcagca
    18301 gtttttctat ttcattaggg cagattccat tgactctttt tgcgttaaga agagggacaa
    18361 aggcagggat gctggcaggt ttcatttggg gcatcctaca ttttccgttg ggacaagttt
    18421 attatttaag tgtcgttcaa gtgttgattg agtacccgat tgcctatact tttgcaggcg
    18481 cagcagggct catggcttca aatgtccaaa aaggctatgt agacgataat acgaaacaaa
    18541 ttagaaaaag catcgtgttt ggtgcattga ttggcgcact ttccagatat ttttggcatt
    18601 ttattgcagg agtggttttc tggggagcgt atgcactatg ggggatgaat ccttggctct
    18661 tttctttcgt aatgaatggt gccagcgggt tgagtacagc tattgtaacg atcatcgttt
    18721 tattagggat ccaacaaacg actccttcga tttttgtgcc agataaacga caggagctgt
    18781 aaataaaaaa gaagcaagaa acttgagaca gaatcatgtc ctagtttctt gtttcctttt
    18841 tattttttga taagaaatag atctacttca atggcagata agaaagatcg gcatcttcta
    18901 ccaattgcca gtgtcgatcg gtgacagagt agattaaagt agcgatgatt tcctcttctc
    18961 gataccgggt gccttttaat ttccaaatat aatcttcaat cacatagatt gggatacgat
    19021 atttaccatc gatcaaacca acttgatcaa aagaaaaaat gatcccttcc tgcatcaaat
    19081 tacgcatttc tttcaataat tccacactga ttttttcaac aggtgtttct tttttgaaaa
    19141 aagatttttt tacattgatc ttatctgaca agatcgaact taatttacta gcaaaaatcc
    19201 tataaaattc atctaaatag cgataataaa tccgattcag ctgatcatta agatcgaagt
    19261 atacaaaatt attctgtagt ttataaaaaa acggcgaatg aagatgtgtt ttcatatgac
    19321 caaaatataa taattctgaa atttctaaag gtgtcaattc tttcagcatc gttaaatcag
    19381 taaagtcgat ccacttcaat tcatcgaatc cacgcctgtt acgagaagag gaaaagaaac
    19441 gctgaatatt ttctgtacca cggattactt tcagccctgt atgggattca tattctccag
    19501 cagaagcaga tggatcaagc aagagcaagt tttgaggaaa atgcacgata ctgtttgcaa
    19561 agtcagtagc agtcagccct tttgttaaaa cagcattact tgtggtatca atatgtacat
    19621 aaactgactc agtcatcatg ttcctcctct tatttcctct taatctcttt tattttacaa
    19681 catttgcatt aaaaaagctt gtttctttta ttttaaaatt ccattacaaa atcacgtttt
    19741 cattgaaaac actctcattt tcaattaatt tttgtgattt ttgtattttt ttatttaaag
    19801 gaggttttat gaattgtttt cttatggaat ctacaaagtc gaatacgaga ctcatgacct
    19861 ttttatgacg tactggtata cttgaagaaa aaggagagcg cgatggagat aaaaaaagtt
    19921 tacttttcac agaaaatgtc gctgtattcg cctatccagc aagcaaaaca accccttcca
    19981 ttttttcaaa gtcatcaaga aagtagagac gctttttggc gttctgcaaa aaagcaatgt
    20041 gcttttaaag cagtatcgaa aaaatatacc gtctatttta ctattgctaa aagtagagga
    20101 gatgttattt ttaataatct gttgattgaa ggtgaaactt ttgaatggaa aaaatttttt
    20161 cggtatatgg aagcttgcgc tcgttttttt attaaagaaa actgttgttt tttatttcaa
    20221 tctcctctat cagaagaatg gttgcgtatt tttcataaaa acggctatca aggaaccaca
    20281 caattaaata aaaagctggt ctatcataca gcacttgttt taggtagagg aggagcgcac
    20341 ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac atgatatcaa ctttgagatt
    20401 atcacaggta cctcggtagg tgcgttgaac ggcgcattga ttttacaagg agatatgaaa
    20461 aaagctgtga atttatggaa aaagctgtcg acaagacaag tgcttgcgtt accagaaatg
    20521 gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa gacgccaaat gacgaaaaca
    20581 gcactaattg aaggcggggt ttcttctgct ccattggaaa agatggtcaa agacaatttg
    20641 gatttgtcct tactcaaaca taatcccaaa atacgtttat atacggtgtc tactaaactg
    20701 ccagaactag cggaagtagt gactgatatc caacagacaa aaacagaaga aataccagat
    20761 tggatactcg cctctgcttc attttatccc gctatggctt atcgaaagat cggaaaaaat
    20821 aaatatgcag acggtgggta tcgcaacaaa atacctattg atattgcgat taacaaagga
    20881 gcaacagagg catttgtcgt agatgtgcaa gggccaggcc cagcgaaaaa aattcgtatg
    20941 ccagacattt ttatccattg gaaatgtcaa acattgtgga cactaggcag ctttctgctt
    21001 tttgatagcc aaaggaacca attaaattta caattaggct atctggaaat gaaaaaacga
    21061 ttaggctatt actatgggaa ctggtataca tttcattctg ttaaacctgc aagaacatac
    21121 tggcgaggat ttttgtctta cttaacaaaa gaaattcagt tagaattatc ttttttcaaa
    21181 agcataaaat tttggcaaaa actgcgtaat ttatataaga acagggtcgt accagagaca
    21241 tgcggattgg cgatgctaga acttttagca aaaaaacatt ttttactgcc caatgaaatc
    21301 tatcaagtag acatgatgat tcaaatgatc tgccaacaag atataaaaag tatgccggat
    21361 gatttattgg cacaaattgg tcaattaagt actgaagaat ggcgaaggta tcgaaaatac
    21421 cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt ttgattatct gctacaagga
    21481 aaacgcaagg atcgtttgca gcatgaattg cttaagcaac cagttgacac attactgatc
    21541 ctatatttat attatctcaa ggaggaacaa ccatggcaca agaattttca tatgaaatcg
    21601 tagaagaaat cgctatattg tcagaaaaca acaaggggtg gcgtaaagaa ctgaacctag
    21661 tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga ttgggcgcca gaccatgaaa
    21721 aaatggggaa aggcctgaca ttgacgaacg aagagtttga acaactgcaa aaagcaatag
    21781 aaaatatgta atgaggaagg accctgtcga atgaaaagta tgactggttt tggcaaagcc
    21841 atccgcgaaa caaaagcgta tcagttagat attgaaataa aaagtgtcaa ccagcgtttt
    21901 ctagatgttc aaatacgaag tcctaaaatc ctgaactttg ttgaaaacga ttttcgtcag
    21961 cttattaaac agcagttaag ccgcggtcga atcgaagtat ttgtgaatct agcttatatt
    22021 ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga ttgattcact tatcactcaa
    22081 ttgacagacg gaattcaaca acgatacggt agtcaaaatc aagtgtcaat tagtcggatg
    22141 gcggaacgct tcgtgctgga cgaaaacttc gtaacggtag aagaaaaacc ggaagagagc
    22201 atggaagaat tggcacagtt agctctagat acattgaaaa gcgcacttga ttctattgag
    22261 gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt tgaaaaagaa tactgaagaa
    22321 ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa tctatgaaaa ggaatttcaa
    22381 ataaggtatc aaaaaaagct ggaggaattt cttggagcga aagtcgatga gcaacggttg
    22441 ttgacggagc tagccatctt gttggaacgc ggagatattc acgaggaact ggatcgtctg
    22501 gcgatccatg tacaaaaact agatgaactt atcgaagcac aatgtcctgt aggtcgggaa
    22561 ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca cgatcggttc caaatcaaat
    22621 gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga tcttagaaaa aatcagagaa
    22681 caagtacaaa atattgaatg acgaaagagg tagagataat gaaatattat gacgtaacgt
    22741 tccacgaatt aagtggaaaa actgtggtta aacgggatat cccttctgaa aaaaacggct
    22801 ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa cgaactgttt attttgatca
    22861 acgacggtgc ttacgtgacg atgaatcgga aatttattgt tcgaattgac acagaagagg
    22921 tagaagatcc gacagaaaaa gcacgttctc gaaaagatga gatcatgggt gtagtgaata
    22981 ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa gaaatacaat aaataaacag
    23041 aattggattt cttcgcttag cttctttgct ctaggaatac atttaaaggt tgtgacaaaa
    23101 ttcgtgtcac aatctttttt tattttgaat aagtgatgtt caaaaaccat tttctcggta
    23161 ttaagccaaa tttatgcaaa aatgtgagaa actattttca caaaattttt cttcatactc
    23221 gaaactaatc ggcttcttca cgacctctta tacacggcgt tcaaaagctt gcatctgcga
    23281 taataacccc aaccaaacga aaaacatgag aagctgttta tgtttggctg ttcttattac
    23341 ttgatgcagg acagcttttt tacgacctct tggtacaggg tagctttttt accaatttct
    23401 agctataatt aaagataacc ttgaaagttg cagaaaaaaa gtgcaaaata aacgataagt
    23461 acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg tggattatta atcgtgttgt
    23521 ctggtccatc cggtgtagga aaaggcacag tacgaaaagc aatttttgag agtgaggata
    23581 atgattttca gtattcgatc tcaatgacta cccgtaaaat gcgtgaaggg gaggtcgaag
    23641 gcgttgatta tttcttccga agcaaagaag agtttgaatc aatgatcgaa gctggagaga
    23701 tgctggaata cgcagaatat gtcgggaatt attatggaac tcctttgtca tatgtcaaca
    23761 agacattgga tgaaggaaaa gatgttttct tggagattga agtccaagga gcaaaacaag
    23821 tcaaagaaaa agtgcccgat ggtgtattca tctttttgac tccgccagat ttagcggaat
    23881 taaaatcacg gatcgtaggc cgaggaacag atgcagatga agtaatcgaa aaacgaatga
    23941 aagttgcaaa agaagaaatc gaaatgatgg ctctttacga ctatgctgtt gtaaatgatg
    24001 aagtgccgct agcagttgaa cgaatcaaaa acattattgc cagtgaacat tttcgagtag
    24061 atcgcgtaat cggtaaatat attaaaatgt taaaggagat gtagtcttat gatgttaaaa
    24121 ccttcaattg actcactatt agatcgtgta aattcaaaat attctctagt gattttagca
    24181 agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta ctctagattc ttttgattca
    24241 gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag gaaatgtagt caatgatcca
    24301 catcctgaat taaaacgtga acgtttgaag atggaagaag aggaacgtca agcacaaaaa
    24361 gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag agcaaaaaat gtaataaaga
    24421 aaaatagagc gaacatctaa aaagaaaagc aaacccagct gtttcctttt caaacattcg
    24481 ccacttattg tctggtagaa aaggctgtgt ctgaactttt tgacacagtt cttttttttc
    24541 ttatctgctg ttaatttttt gttaggattt ggcagaagat aagacaaatc catcgatctc
    24601 tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa aagacaaaaa aaggaggaaa
    24661 cgaaccatga cattaacagc tgaagtcatt gtggacgtac ctactatgca gaccgatcag
    24721 ccattcactt atttggttcc ctctgaaatg gaaacagctt tgcaagtcgg gatgcgagta
    24781 gaagtccctt ttggtaatgg caatcgtcat gtccaaggat ttgttatggc tatccaaaaa
    24841 tcagaagaat cagctaatcc atctttgaag gcagttattc gtttgctaga ccttgctccg
    24901 gtggtcaatg aagaattact ttcattagct gattatatga aaaaaattac ttacgcattc
    24961 aaaatcactt gtctacaaac gatgcttccg agtgtgatga aagcagaata tgataaattg
    25021 atctatcctt tggcagacac accagaagtt caggtattgt ttgatcagcg agaagtgatt
    25081 tcctggaagg aagcagaaga agaaggcagc ctttctcaat tgattcgctg gcgccaagag
    25141 caattagtag atatcaaata tgaagtccat acacgaaata aagtaaaaac gattcgtttg
    25201 gtccgttctt tgttaactga aaagcaaatc gaagaagaat gggcaaaatt acggcaaaat
    25261 gccaagaaac aaaaagaact tcttttgtgt ttatctgaac tgagccaaga agaaccaatt
    25321 gcttatttca aaaataaagg aatcagtact gctgtcttga atcaaggaaa agaaaagggc
    25381 tggctggagt ttatagaatc agaacgttat cgcgaccctt ataaagaccg agtatttgac
    25441 caaacgacgg cattggaact gaatgcagaa caaaaaaatg ctgtcgaaca aatcatcaca
    25501 gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg gaatcactgg cagcgggaaa
    25561 acagaagtat atcttcaggc aatcgctgat gttttgtcag aaaataaaac ggctattatg
    25621 ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc gattcaaagg acgcttcgga
    25681 gaatcagttg cggtcttaca tagcggactt tcccaaggtg aaaaatatga tgaatggcgc
    25741 aagattgagc gggaagaagc gcaagtagta gtaggcgcac gatctgctat ttttgccccg
    25801 ctgaaaaata tcgggctgat cattatcgat gaagaacatg aatcaagtta caaacaagat
    25861 gagacaccgc gttatcatgc gagagatcta gcaatttggc gcagtaaata tcatcattgc
    25921 ccaatagttc taggaagcgc gacgccctct ttagaatcaa gagcacgagc tcaaaaaggc
    25981 gtttatcagt tattgcagtt aaaccaccga gccaaagcag ctgcacagct tccagctatt
    26041 gagatcgtag atatgcgtga agagtttcag aatcatcgga cctctacttt ttctgctaat
    26101 ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac aaactgtcct actgctcaat
    26161 cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg gatttgttct tccatgtcct
    26221 aattgcgata tctctcttac actgcatatg gatacacgtt caatgcgctg ccattattgc
    26281 ggacatgaag aacctattcc caatcgctgt cctaattgtg gcggcaataa aatccgctac
    26341 tatggaacag gtactcaaaa agtagaagag gaactccgag aactttatcc gcaagctagg
    26401 attttgcgta tggatgtaga taccactcga agaaaagggg cacacgaaca gattttacaa
    26461 aaattcggtg caaaagaagc agatatatta ttagggacac aaatgattgc taaaggtttg
    26521 gactttccag aagtgacatt agttggggtg ttgaatgctg atacctccct gaatttaccg
    26581 gattttcgtt ctagtgagca tacattccaa ttgcttaccc aagtttcagg acgagcaggg
    26641 cgagcggaaa aagcaggcga agtcgtgatc caaacattca atcctcaaca ttacgccata
    26701 gaattggcta aaaaacaaaa ttatgagcag ttttatcagc aggaaatgca tgtacggcat
    26761 cgtggaggct atcctcctta ttactttact gtgaaaataa ccgccagcca tcctgaagaa
    26821 caagtagcgg caaagaaaat ctttcagatt gccaatcagc taaaagaagt gttaagccct
    26881 caaagcttgc tattagggcc aacgcccagc atgattttga gagtgaaaaa ccgatattat
    26941 tatcaactaa tcatcaaata taaacatgaa ccgaatcttc ctcatgtttt ggatgaaatt
    27001 ttgaatggaa gccagaaaga acagcgacaa ggtctttttg tggcaattga caacgaacca
    27061 ttaaacttta tttaagaaag ggccaaatta tgcgctatcc tatattgatc caccctaatg
    27121 ataaattaaa aagaactgct cagccaatcg atgtaatcac ggacgagaca atcgctttat
    27181 tagacaattt atatgagaca atgattgcga atgacggaat agggatcgct gctcctcaag
    27241 taggacaaaa caagagaatt gctgtgatcg aagtagatga aggcgagaaa tttgaactga
    27301 ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga cgtagaaggt tgtctcagta
    27361 ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt gaccgtgcgt tattatgatc
    27421 gagacggaga agagatagaa gtgacggctt ttggctattt agcccgtgcc ttccagcatg
    27481 aaatcgatca cttagacggt atcttgttca ttgagaaaat gatacagcag atcccggaag
    27541 aagaacttga agaatatatg gaggaacatc ttgatgacta agctagtatt tatgggcaca
    27601 cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat caggttacga gatccaggca
    27661 gtggtaacac agcccgaccg tccagttggg cggaaacgag taattacgcc gacaccggta
    27721 aaaaaagcgg ctctgaagca cggtatccgt gtgctgcagc cagagaaaat ctccggttcg
    27781 ccagaaatgg aagaaatcat tgaattggtg cctgatgtga tcgtcactgc ggcatttggc
    27841 caattcttgc ctgaaaagtt attgcaagta ccaaaattag gggcaatcaa tgtccatgct
    27901 tctttacttc ctaaatatcg gggaggtgcg cctgttcatt atgccatcat gaatggggag
    27961 aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa tggatgcagg cggaatctat
    28021 gcgcaagaaa gcataccaat cactaaacaa gatgatgtag ggacgatgtt tgaaaagctt
    28081 agccttttag gaagaaagct attgctagaa acattgccca atattttgga tggacaaaaa
    28141 ccagtacctc aagacgagtc agaagcaact ttttcgccaa acatcacacg ggaacaagaa
    28201 gcaatcgatt ggcataaaac tgcagaagaa attgacaacc aagtacgcgg aatgcgtcct
    28261 tggccaattg cattcacgac ctatgagggc atacgctgga aattattatc ggttgaagta
    28321 ttggcagaaa ctacagacaa agcacctggt acgatcatcc ggaaagacaa aaaaaatcta
    28381 tggattgctt gcggtaaaca gaccgtctta gccatcaatg aattacagcc agctggtaaa
    28441 ggaaaacagg ccgtacatga tttccttaac ggaagcggtc agcaagttca gattggtcag
    28501 caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa atcagtacga tatatggcat
    28561 tactgacatt agagcgagtg aacaagggtg gcgcctactc gaatcttttg ttgaatgaga
    28621 tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt atttacagaa ttgatctatg
    28681 gtacgattag ccatcagcga ctattggaat tttacgttgc accattgatc gctaaagcaa
    28741 aaaaagtgga tgactgggtc aagatgcttc tttatctttc tgtataccaa ttagaatttt
    28801 tagataaagt ccctgcacat gctattttaa acgaggcggt cgaaatcgca aaagtaaaag
    28861 gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag gaattatcag cgtcaaggag
    28921 caccagatct gcaaggcatt gctgatccta tcgaaagact ttctgtcaaa atcagcctgc
    28981 ctctatggat gacgaaacga ctggtggatc aaatcggata cgaagaaact gaaaagctcg
    29041 gtttatcgct atatcagcct agccatgcta gtgcccgagt agatacacgc cgactttcca
    29101 gagatcaggc catcactgtt ttgcaagaag aagagatcac agctgaaaaa agccaagtca
    29161 gtccatacgg tatagtagcg aaaaaagggc atttagcttc tagctcattg ttccacgatg
    29221 gcgtcatgac gatccaagat gaaagttcga tgcttgtagc gccgtctatg caaatcgagc
    29281 ctgaacatca tgtcttagat gcctgtgcag cacccggagg taagacgacg catattgctt
    29341 cttttttaga tccgtcatgc ggtggagaag tggtcgcttt agatgtacac gaacacaaag
    29401 tcaaactcat tgaagataat gcagcacgtc taggtgtctc ttctgctgtc tacgctcaaa
    29461 agatggatgc tagagaagtc agtaaacagt tcgaagcaga gcagtttgat cggattttag
    29521 tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa accagatatt cgttataaaa
    29581 aacagccgga tgaattagcg aatttaccaa aaatccaatt ggaaatttta gaaagctgcg
    29641 caccaacttt aaagtcttcc ggtattctga cttatagcac ttgtacgatc ttaaaagaag
    29701 aaaaccaaga cgttgtttct gctttcttgg aaagacaccc tgattttgag aggattgatg
    29761 ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa aatgttgact ttgtatccgc
    29821 atcagtttta cacagatgga tttttcattt gttgtttgcg caaaaagtaa tgaggtgaaa
    29881 aaatgcagat tgaatatcaa tcagatgtag gaagacgacg taacacaaat caagactatg
    29941 ccagtgtatt taccagccgt tcaggtctca agcttgctgt attagcagac gggatgggtg
    30001 gtcatcgtgc cggagacatt gccagtcaga tggcggtaac gaatctaggc gaagcttggg
    30061 aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg gttcattcaa tcgattcaag
    30121 aagaaaatat gctgatttat cagcgtggac aagaacagcc ggaatataac ggtatgggaa
    30181 caacaatcgt agcggccgct ctgtcagatg actggttcac gattgccaat gttggggata
    30241 gcagagctta tttgattcgt gaagataaaa tcatgcaatt gacggaggat cattctttag
    30301 tcaatgaact agtcaaatca ggagaaatca gcgaagaaat ggcagtgaac catccaagga
    30361 aaaatatttt aacacgttct gtcggcatgc ctggaacagt cgaagtagat gtttctactc
    30421 atctttggca actaaaagat cgactgctgt tatgttcaga tggcttgacg aacatgctgt
    30481 ctgaagaaga aataggaacg atcatcaatc aagaaggccc attgtctcat aaagtaactg
    30541 agctgattga tcgtgccaat gaagcaggcg gagcggataa tatcaccgtt cttcttatcg
    30601 aatataagga ggacagtgta tgatcgagtt gggcaaaaaa ttaaatggcc gttatcaaat
    30661 cactggaaat atcggtagcg gtggtatggc aaatgtattt ttggcgcacg atttaatctt
    30721 ggatcgtgat gtagcagtta aagtcttacg atttgacttt caaaatgacc agactgcgat
    30781 tcgacgattt cagagagaag ctttagcagc aacagaactc gttcatccaa acattgttag
    30841 tgtttacgat gttggtgaag aagacaacat gcagtatctg gtgatggaat acgtcaaagg
    30901 aatggacttg aaacgatata tccaaacaca ttatcctgtt ccttatgaaa cagcagtaaa
    30961 tattatgcag cagatccttt ctgctatttc tttagctcat aaccatcaaa tcatccaccg
    31021 agatttgaaa ccccaaaacg tactgatcga taatgaaggt gttgtaaaaa tcacggattt
    31081 cgggattgct atcgctttat ctgaaacatc gatcacgcag acgaatacga tgctaggatc
    31141 tgttcattat ctgtcccctg agcaggccag aggaagtatg gcaacgaagc agtcagatat
    31201 ttatgcttta gggatcatcc tttatgagat gttgacgggt agcgtaccgt ttgatggtga
    31261 atctgctgta acaattgctt taaaacattt tcaagatgat cttccatcta taaaagcact
    31321 tgatccaaat gtgccacaag cactggaaaa cgtgatttta cgggcaacgg ctaaagaacc
    31381 tgcagaccgt tataagtcag ccgaggaaat gtctgatgat ctttctacag tattatcacc
    31441 agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg gataatgaaa caaaagttat
    31501 cacacccctt acggaagatg caccaatgcc tgattcattc aaatctatgc ctctgccaaa
    31561 agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa gttctgcctg atcctaaaaa
    31621 gaaaggacag aaaaaatggt ggctgatcct attagctatt cttgctgttt taggaatcgg
    31681 aacagctgta ttctttgctt ctggcgggcg aggagaagtt gttgttcctg atgtttctga
    31741 tctttctgaa gcttctgcta gagaaacact cgcaaaagcg aaattagaag tagcaacaaa
    31801 aacagaagaa attgcagatg acaaaatcga agaaggtaat gtagtgaaga cagacccggc
    31861 agcgggaacg acagtcaagc aaaaacgcga agtcactctg tatattagta gcggcacgaa
    31921 aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa gatgccgaag caagtttgaa
    31981 agaattaggg ttttctgctg acttgattca aaaagaagag gaattcagtt cagaggtaga
    32041 aagcggaatg atcatttcgc aatctccatc agaagataca gaagtcgatc ctaaaacaga
    32101 cacgattact ttcacagtca gcaaaggacc agaaacagtg actttgagta gtcatgtagg
    32161 agaaaactac gaaactgttc gtaattggct tgttacacaa ggattcaata tcaataatat
    32221 tagtgctact tatgactata gcgacactgt accatcaggt tcgatcatca gtcaggatcc
    32281 agcctcgggt gaagttgtag cagaagaaac ttacatcaat tttgtcgtaa gccaaggaac
    32341 agagcctaaa ttgaatgata tctccggcta tacaaaaagc gaagcacaaa gttatttagc
    32401 tagtgtcggt gcagaatata tcgggcatga gacttccgaa tattcaaaca cggtggataa
    32461 agataaagtg attcgtacag taccaggggc aggaacgaca attaccaaag gaatggttgt
    32521 gaacgtaatt tactctaaag gaccagatcc agcgtcttct agttctgaaa aagactcttc
    32581 agacagttct agcagctcaa gtagctccag tagcacaagc aactcttcaa aagaagaatc
    32641 ttccagctcc acaagttcat cagctagcac ctcagaaagt tcaaaataag tgctgaataa
    32701 aatatatcag aagggaatgc gacattgatt tgttgcattc tcttttttgt gtttctatct
    32761 cttttgatca attttcaagc catatacttt gagaagtttg catggaataa cacctacttt
    32821 cctcttcgta tggtaaaata aacaaaaacg gaggtggcac ttatcaaagg acaaatcaga
    32881 aaagcaatca gcggttttta ctatgtatac gcaaacggag agacctatca aacaagagga
    32941 agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg gagatcaggt gatctttgag
    33001 agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc cgagaaaaaa tcaattagtt
    33061 cgcccaccag tcacaaatgt tgatcttgga atcattgtca caagtatcgt agagcctgca
    33121 ttttcttata atctacttga ccgctttcta gttaccttgg aatacgagtc gatcgaaccg
    33181 attatttttt tgacaaagac cgacttgtca gacgacccag aaaaaatcga agagatcaag
    33241 caaacctata ctaaaatagg ctatccggtg attgtaccca atcatacggg tgatacgaca
    33301 gaactgatcc gttacttccc agaacggcta tcggtcttta tgggtcagtc tggagcagga
    33361 aaatcgacgc tactgaatca aatatcaccg gaactgaatt tagcaacagg agagatttct
    33421 gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg aactgattcc tatctatggc
    33481 gggttagtag cagatacacc aggatttagc tcgattgact ttttgacaat tgaagcagga
    33541 gaactgccga gacaatttcc tgaatttgta gaagcttctg tccattgcaa atttagagag
    33601 tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata gagtagaaac aggtggtatc
    33661 gcacagacac gttacgacaa ttatttgcaa ttcttacaag aaatcgaaaa aagaaaacca
    33721 atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa caagaatgaa gatagcacca
    33781 tccattttga gtgcagattt tgccaattta caaagagaca ttgagttagt ggaaaaagga
    33841 ggcgcagatt acatccacgt agatgtaatg gatggccgat ttgtcccaaa tatcacatta
    33901 gggccaaata ttgttcaggc aatccgtcct gttactaaat taccgttaga cgtccatctg
    33961 atgatcgttg atccagaaaa ctacattcca gcatttgcaa aagctggagc agatatcatt
    34021 actgtccacg tcgaagcaac tcctcatatt catcgggcac ttcaaatgac aaaaagctta
    34081 ggagttaaag ctggagtagt tatcaatcca ggaacaccag tctccatgat caaacatgtt
    34141 ttgcctttgg ctgatcaagt actagtcatg actgtcaatc ctgggttcgg cggacaaagc
    34201 tttattgaag aaacagtaga aaaaattacg gaactcgctg atctacgtag agagaacaat
    34261 tggacttatg caatcgaagt agatggcgga atcgttccag aaacagcaaa aatctgtaaa
    34321 gatgcagggg cggatgtctt cgttgcagga tcatatatct atggagccga aaatccagtc
    34381 ggacaaatcg aacgattaaa agaggcatta gactaaatga aaattttatt agctgctgga
    34441 ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat acgatgccta tgtcgggata
    34501 gaccgtggtg cgctgtacct gcttcaagca ggctacccag tagatctggc agtgggtgat
    34561 tttgattcat tgtcgaacga agagcagaag gaagtatttg caaaagccaa agagtatacc
    34621 cgttcccctg cagaaaaaga cgatacagat acacagttag gtatcttaaa aacatttgaa
    34681 cgttacccgc aagcggaagt cactttgatt ggagctacgg gcggaagatt ggatcatctt
    34741 ttagccaatc tctggttagg tatggaacct cgcttcaaac cctttgccca tcagcttatt
    34801 atcagagatt gtcaaaatta tttgtcttat tatttgcctg gagagcatat agtaagacag
    34861 ataccagata tgaagtattt agcttattgc tgcttgactc ccgtctcgaa tcttacatta
    34921 aaaggcagca agtatgtatt ggaaaataaa gaagtagaat atccgacatc ttatgcaagt
    34981 aacgaatttc tttcagatac cgcagaattt tctttttcag atggtatgat tgcagttatt
    35041 caaagcaaag attgatccat aaacaaataa aaaaatttgt ctttcaggct aaaaatgaca
    35101 gaatctatta tttgctaaaa aaaagcttaa gaggagcaac ttaccttttg tttcacggca
    35161 tttagtgata aaattgaaca caagagtcta attaattcga ggtgtctttg tgaagaagtt
    35221 caatcctaat aaaaatatta ttattacctt ggttattgtt atcatcgtgg tgacgatact
    35281 aagtgtgacc gctgcgcagc gagcgaatga aggaaagccc aatttcttcc aatccatcgt
    35341 taatgacagt gttgcaatgg ttgataaaac aatctctgcc ccagtacggt gggtacgaaa
    35401 tggagtagat tctgtccata atcttttttc aacttatgcc gaaaatgagc ggctaaaaga
    35461 aaaaatcgat agctatgatg acgtttcaca gcaaaacaag aattacgaga gagaaatcga
    35521 agcgttgaaa aaagaacttg agttgaatca gacgctgact agttacgaaa aagtaacggc
    35581 aaatgtcatt actcgttcgc ctgacagttg gcaggacatg ctgatcgtag acaagggttc
    35641 taaagacggt atcgaagcaa atatggcagt catgtcgcaa aaaggattga ttggccgagt
    35701 gatcgaggtc aatacggctt cgtctaaaat cgaattactg tcatcctcta atgaaagctc
    35761 caatcatttt ccagtacggg tatcttcggc taatggcgaa gcgtttggtt tgcttaaaaa
    35821 ctatgatgaa aagctccatg ccttagtggt gacccaatta actggtgata cggatatcaa
    35881 agaaggggat gttgtccaga catccggtct tggagggaat tctccagcta acttgccgat
    35941 cggtacggtt attaaaacga aaccagatag ttatgggctg gatcgggaag tttatgtgaa
    36001 accttatgca gaaatgtatg acgtgtcagt tgtgacgatt gtccaacggc tagcggagga
    36061 ttagtccatg ttgaagaaag aaacaatgaa gtactatctg ccaatcgttt tgttcttttt
    36121 gatgttgata gatggtcatt taacaagaat gctaggggag tggtcgaaag gcacctatat
    36181 gtcaaatgcc cactttctga tattggcatt attatgttgc agtatggcgt ttgaaaaacg
    36241 ttatttactg attaccacga ttgttctcgg ggctatctat gacgcttact atattggcgt
    36301 tatcggtatc tatgcagtag ctctcccttt aattgtatgg ttgatgtatg taatgaaaga
    36361 cgttatccat gtcaacatct ttactgaatt tttcagtatg atcatctttg tcacgggtta
    36421 tgaattgttt acgatggtgg tccagttgat ttttaaatta gcagtagtaa ataacacgta
    36481 ttttattaca aggtttttag gacctacact gctgttgaac atgattatat ttgtattatt
    36541 catttttccc tttaagaaat tattcagtga tgaataattt ctttttttat ttttaggtcg
    36601 aaagcgaaaa agaatcgttg ttatcttaag aaatgctaga cttaacgcgg ctttttcatg
    36661 atcattttct gaaacgaaca gttttgtcat ttttaaaagt tgttttgaaa catttttgta
    36721 acataactat tatatcgttg acacactgct atggtacaat ttttatatcg ttaagaaaga
    36781 cttaaatata tttagatatt tattaaatta agccagtcgg aggaatgaag agtgaaaaag
    36841 agtttaatat cagcagtaat ggtatgttca atgaccttga ctgccgtagc atcaccgatc
    36901 gctgccgcgg cggacgattt tgattctcag atacaacagc aagatcaaaa gattgcagac
    36961 ttgaaaaatc aacaagcaga tgcacaatca caaatcgatg ccttagaaag ccaagtttct
    37021 gaaatcaaca cacaagcaca agacctatta gcaaaacaag atacattgcg tcaagaatct
    37081 gcacaattag taaaagacat tgcagattta caagaacgta ttgaaaaacg tgaagataca
    37141 atccaaaaac aagcacgcga agcacaagta agcaacacaa gctcaaacta catcgatgct
    37201 gtattgaatg ctgattcatt agcagatgct attggacgcg ttcaagcaat gacaacgatg
    37261 gtaaaagcaa acaacgactt gatggaacaa caaaaacaag ataaaaaagc tgtagaagac
    37321 aaaaaagctg aaaacgatgc taaactaaaa gaattagctg aaaaccaagc tgctttggaa
    37381 tctcaaaaag gcgatctttt atctaaacaa gctgatttga acgtactgaa aacttcatta
    37441 gctgctgaac aagcaactgc tgaagacaag aaagctgact tgaaccgtca aaaagcagaa
    37501 gctgaagcag agcaagctcg tattcgtgaa caacaacgtc tagcagaaca agctcgtcaa
    37561 caagctgcac aagaaaaagc tgaaaaagaa gcacgcgaac aagcagaggc tgaagcacaa
    37621 gctactcaag cttcatctac tgcacaaagc tcagcaacag aagaatcatc agctacacaa
    37681 tcttcaatga ctgaagaatc atcatcagct acacaatctt cagcaacaga agaatcaaca
    37741 acacctgaat cttcaacaga agaatcaaca gcgcctgaat cttcagcaac agaagaatca
    37801 acaacagcgc ctgaatcttc agcaacagaa gaatcaacaa cagtgcctga atcttcagca
    37861 acagaagaat caacaacagt gcctgaatct tcaacaacag aagaatcaac aacacctgcg
    37921 cctacaacac catcaacaga tcaaagtgtt gatacaggaa acggcacagg aagtagtact
    37981 ccggctccaa cgccaacacc aacacctgaa caaccaaaac ctgtaacacc tgctccagct
    38041 ccttctggaa gtgtgaatgg tgcagcaatc gtagcagaag cttacaaata tattggtact
    38101 ccatatgttt ggggcggtaa agatccaagt ggctttgact gctcagggtt cacacgctat
    38161 gtttacatgc aagtaactgg tcgtgacatc ggtggttgga cagttcctca agaatcagct
    38221 ggaacaaaaa tttcagtttc tcaagcaaaa gctggagact tgttattctg ggggtcacaa
    38281 ggcggaactt accacgtagc gattgcttta ggcggaggac aatatatcca tgctcctcaa
    38341 ccaggcgaaa gcgtaaaagt tggttcagtt caatggtttg cccctgactt tgctgtcagc
    38401 atgtaagaat ga
//
LOCUS       DAYYNQ010000015        35478 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000015 DAYYNQ010000000
VERSION     DAYYNQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.015"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..774)
                     /locus_tag="ACHBJ1_000711"
     CDS             complement(34..774)
                     /locus_tag="ACHBJ1_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429678.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(904..1851)
                     /locus_tag="ACHBJ1_000712"
     CDS             complement(904..1851)
                     /locus_tag="ACHBJ1_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9429679.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(1980..2744)
                     /locus_tag="ACHBJ1_000713"
     CDS             complement(1980..2744)
                     /locus_tag="ACHBJ1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9429680.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2748..3137)
                     /locus_tag="ACHBJ1_000714"
     CDS             complement(2748..3137)
                     /locus_tag="ACHBJ1_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429681.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3272..3814
                     /locus_tag="ACHBJ1_000715"
     CDS             3272..3814
                     /locus_tag="ACHBJ1_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM9429682.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3962..4594
                     /locus_tag="ACHBJ1_000716"
     CDS             3962..4594
                     /locus_tag="ACHBJ1_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429683.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4728..4853)
                     /locus_tag="ACHBJ1_000717"
     CDS             complement(4728..4853)
                     /locus_tag="ACHBJ1_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM9429684.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(4987..5880)
                     /locus_tag="ACHBJ1_000718"
     CDS             complement(4987..5880)
                     /locus_tag="ACHBJ1_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM9429685.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6062..6988)
                     /locus_tag="ACHBJ1_000719"
     CDS             complement(6062..6988)
                     /locus_tag="ACHBJ1_000719"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM9429686.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7073..7834)
                     /gene="pflA"
                     /locus_tag="ACHBJ1_000720"
     CDS             complement(7073..7834)
                     /gene="pflA"
                     /locus_tag="ACHBJ1_000720"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM9429687.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8078..10309)
                     /gene="pflB"
                     /locus_tag="ACHBJ1_000721"
     CDS             complement(8078..10309)
                     /gene="pflB"
                     /locus_tag="ACHBJ1_000721"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM9429688.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10586..13036)
                     /gene="parC"
                     /locus_tag="ACHBJ1_000722"
     CDS             complement(10586..13036)
                     /gene="parC"
                     /locus_tag="ACHBJ1_000722"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM9429689.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13048..15093)
                     /gene="parE"
                     /locus_tag="ACHBJ1_000723"
     CDS             complement(13048..15093)
                     /gene="parE"
                     /locus_tag="ACHBJ1_000723"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM9429690.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15259..15693)
                     /locus_tag="ACHBJ1_000724"
     CDS             complement(15259..15693)
                     /locus_tag="ACHBJ1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM9429691.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15820..16458
                     /gene="plsY"
                     /locus_tag="ACHBJ1_000725"
     CDS             15820..16458
                     /gene="plsY"
                     /locus_tag="ACHBJ1_000725"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM9429692.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16590..17465)
                     /locus_tag="ACHBJ1_000726"
     CDS             complement(16590..17465)
                     /locus_tag="ACHBJ1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM9429693.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17547..18338)
                     /gene="codY"
                     /locus_tag="ACHBJ1_000727"
     CDS             complement(17547..18338)
                     /gene="codY"
                     /locus_tag="ACHBJ1_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM9429694.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18356..19756)
                     /gene="hslU"
                     /locus_tag="ACHBJ1_000728"
     CDS             complement(18356..19756)
                     /gene="hslU"
                     /locus_tag="ACHBJ1_000728"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM9429695.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19770..20318)
                     /gene="hslV"
                     /locus_tag="ACHBJ1_000729"
     CDS             complement(19770..20318)
                     /gene="hslV"
                     /locus_tag="ACHBJ1_000729"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM9429696.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20355..21260)
                     /gene="xerC"
                     /locus_tag="ACHBJ1_000730"
     CDS             complement(20355..21260)
                     /gene="xerC"
                     /locus_tag="ACHBJ1_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM9429697.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21458..22834)
                     /gene="trmFO"
                     /locus_tag="ACHBJ1_000731"
     CDS             complement(21458..22834)
                     /gene="trmFO"
                     /locus_tag="ACHBJ1_000731"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM9429698.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22803..24881)
                     /gene="topA"
                     /locus_tag="ACHBJ1_000732"
     CDS             complement(22803..24881)
                     /gene="topA"
                     /locus_tag="ACHBJ1_000732"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM9429699.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25003..25860)
                     /gene="dprA"
                     /locus_tag="ACHBJ1_000733"
     CDS             complement(25003..25860)
                     /gene="dprA"
                     /locus_tag="ACHBJ1_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM9429700.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25916..26683)
                     /locus_tag="ACHBJ1_000734"
     CDS             complement(25916..26683)
                     /locus_tag="ACHBJ1_000734"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM9429701.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26676..27536)
                     /gene="ylqF"
                     /locus_tag="ACHBJ1_000735"
     CDS             complement(26676..27536)
                     /gene="ylqF"
                     /locus_tag="ACHBJ1_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM9429702.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27796..28194
                     /locus_tag="ACHBJ1_000736"
     CDS             27796..28194
                     /locus_tag="ACHBJ1_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9429703.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28431..28973
                     /locus_tag="ACHBJ1_000737"
     CDS             28431..28973
                     /locus_tag="ACHBJ1_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9429704.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(29056..29607)
                     /gene="lepB"
                     /locus_tag="ACHBJ1_000738"
     CDS             complement(29056..29607)
                     /gene="lepB"
                     /locus_tag="ACHBJ1_000738"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9429705.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29759..29986)
                     /locus_tag="ACHBJ1_000739"
     CDS             complement(29759..29986)
                     /locus_tag="ACHBJ1_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM9429706.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(29983..30501)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_000740"
     CDS             complement(29983..30501)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_000740"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9429707.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30521..31234)
                     /locus_tag="ACHBJ1_000741"
     CDS             complement(30521..31234)
                     /locus_tag="ACHBJ1_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM9429708.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31237..32133)
                     /locus_tag="ACHBJ1_000742"
     CDS             complement(31237..32133)
                     /locus_tag="ACHBJ1_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9429709.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32291..33133)
                     /locus_tag="ACHBJ1_000743"
     CDS             complement(32291..33133)
                     /locus_tag="ACHBJ1_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9429710.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33265..33918
                     /locus_tag="ACHBJ1_000744"
     CDS             33265..33918
                     /locus_tag="ACHBJ1_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM9429711.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(33981..34508)
                     /locus_tag="ACHBJ1_000745"
     CDS             complement(33981..34508)
                     /locus_tag="ACHBJ1_000745"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9429712.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34528..35475)
                     /locus_tag="ACHBJ1_000746"
     CDS             complement(34528..35475)
                     /locus_tag="ACHBJ1_000746"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM9429713.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
BASE COUNT         9490 a         7406 c         6124 g        12458 t
ORIGIN      
        1 ccaactgtag ccgaatataa atttgtatat cacttattta aaatagctat tttctcccaa
       61 agagtttcct tgatcatcga tcaatccttg taatgagcgt tctaagatac catggctttt
      121 tttcgatttt ttttcttctg actgctttgt ttcctctaac gtttttgttc gattgaatgt
      181 tcgtacagac ggctcaccat ttcttttttc ttgatacggc gtcggttctg tatcgaacaa
      241 cagataagag tcatccgttt ttttcatcga ttcgattaaa tgatcctcag aaattttgct
      301 ttctctaggg tctgggataa tcgatgcggg tacatattta ggaacaaaat aagaacgctc
      361 ccgcaccttc gtgtcaacag atgcgtttcg tgtagtatgc ttggactctg tccttgtttc
      421 actttgtctg acagaacgag aggaatcacc aaaaagtttt tttcgtcctg tcgatgtgat
      481 ttctgattgg taatggccgg tataatccgg taattttcct ttatgctttt ccaaattttc
      541 cttacgcgga gctgacttca atccatttgt tgcacgacgc ttgcgacgga ttttctcggt
      601 gttcgtgttc gtgaaatgaa attctttcat ctgttcaaac ggggactgat tctgttcagt
      661 caaaagatct tcttgtccgt caaataatac tcgcttcttg tcgttggtta attttacgcc
      721 taactcatca tcgtacgcag gaaaacggaa atgttttcgt ctgatttctt tcattcaaac
      781 catccttctc aaaaatggaa cttttctcaa taatagcaca gattcattgt tttcagtagc
      841 aatgcgctga ttattttaag aaaatagcga tgaatttcat aaatatatat atagtaataa
      901 gacttaattc gttgtctctc tgataactag ttcaggaaaa aaacgacgat aaccttgact
      961 cgcccctttt tcttctattt tttttaacaa catttctcca catacttcac ccatacgtat
     1021 aatatcttgc ttgatcgttg tcaatttagg tgaactgatt tggtctaaaa ataccccatc
     1081 aaatccaatc acaccaaatt cttcgggcaa gtgtcctcca gcatttataa tccctcttac
     1141 tgctcctagt gctagccgat ctgaactaca tacaaaacaa gttttatctg taaaatctgc
     1201 ccagttttct gtcatatatc tttctacttc tgtcgaatga tttggaaacc gcaagatttt
     1261 actaggaaga tcaacttcct tcatcacaga taaataacca gtttcccttg aaatttcaaa
     1321 aggctcgtcc acttcgatac cgatataaat aatcgtttga taatctcttt ctaaggcata
     1381 ttgcgttgct tttgctgtac catattcatt atcgctgtct acataatcat atccttcaga
     1441 attttcccca aacaagacaa ctggtttttc caaacggtcg atccaatcga agtctttttt
     1501 tctgacacct gtgatgatgt atccatcaca agaaccgacg tcgaaggcat catttgtaac
     1561 taattggaga gaataatgcg ctttaccgat ccgtttagcg attcccatca gcaaattcat
     1621 gtaatagggc tcagttgtgt cgatttcttc cagtataaat aatttgatga tctgcgaccg
     1681 attcgtgaca agtgcttttg cagcaatatt tggacggtaa tccagttcag ccattgctga
     1741 gaaaactaac tctttcaatt cgtcagtcac ctgttccgga tgattgatga ctcgagagac
     1801 cgtcattttt gaaacattgg cacgttttgc aacatctgct aaagtgggca tgctctcttc
     1861 ctttctaaca tgttgtcatt cttactatca attataccaa attttcctaa cagagtttat
     1921 tatagcccaa aataaaaaac ggaaaatcct ctatcgtcat gagtgatttt ccgtttcaat
     1981 tatttatact tttttatcaa attttttgct aaaaaagtgg aagcttcttc cattagttgt
     2041 atttgtacca tatgtcgctg tgtttttgag gaacgaaaaa caatttcttt tccgtaagac
     2101 ttatgccgat ttttttctac aaatcgttcc gtttgtatat aagggatttt ttcgtcagct
     2161 tgtccatgcc aaaagaacag aggacgtcca gctaactttt ccggctgcaa tgacaaatca
     2221 tagctcttaa cccatgaaat caaatcaaaa tagtcttttg gtacgacata attatattcc
     2281 gatgcgcgcc ttacgatttc ttttccatat tccaaaggtg caggggaacc catcaaacac
     2341 acgcccgcta ctatatgagg atgtttagcc aacaaggcgc atgttgtcat tccccccatc
     2401 gaaaccccgc caactgcaag ctgatcatgt agaagacctc ttttttctaa atgcttgata
     2461 agtgtgtcaa attcaaacag atttccgtag atactatccc aaaatgtata ggatggaata
     2521 gaagaaaccg gctgtttgcg ctccccatga ttcatcgcat ctggcaggat cacacgaatg
     2581 ccctgctgcg caatttttct tccttgcgtc aaaactaact ccttacttgt ctgccatccg
     2641 tgataataga tgacaacggg aatacgttcc tctcttaatt ctttcagaac aacttctaac
     2701 actggaatat gatggacaga acgtttcaat ataattggat tcaattgtta ctcacctatt
     2761 ttcattaaat ttcctgatcg ccatagcaac catgctccaa cagttgtctg gaacaggttc
     2821 agtgtaccaa ataatataaa tgcaggactg aatggctcgg gaaataagca cagagaacaa
     2881 agtcccacca ctcctaatgc tagcgaaagg agtggataaa tagaaaaatt caaaaaactg
     2941 gcaacgctac cgaaaatatt accaataaac agtatcaaaa agaaaaaaac gcttaaaatt
     3001 aaaccatctg tcagcgacat ctgaaaaaca atgttctcta cctcaattgg ctgttgaatc
     3061 gaatagacac ctaagtatat actgagaagg agacttagca cgtggctggc acaaaacaca
     3121 cgattgaccg ttttcatttt aatcacctaa cctatcttta taattctatt ataatcattt
     3181 tatcattttt ttgtatgtat aaagtttcaa tttttcttca cttacgattt tttttgatat
     3241 tgttgttata taaatgaaag gagttttaca aatgattgat ccaaaaatgt tggggatgat
     3301 cttcctcatt aattttgctt atatcactct gaacacgtta cgtttcatgt tgacaatgaa
     3361 aggctatcga ctgattgctc cacttgtcag tatggtcgag atcacgattt atatcgtcgg
     3421 cttatccctt gttttagatc gtttagataa tccactgaat ttatttgtct atgcgttagg
     3481 ttacgcagta ggtatcagtg tcggaatttt gattgaagac aaattggcat taggatacat
     3541 catggtcaca acgattttgc cagcaaatag cgcagaagat aaaagtttgc cgggtatact
     3601 gagagaacac ggttatggtg tgacacagac ttctgcaatg gggctggaag gtgagcggct
     3661 agttctcgaa atactttctc ctagaaaaag cgaacgtgaa ctgtatcgcc tgatcaaaga
     3721 aatcgaagag cgtgctttta tcatttctta tgagccaaaa tatatttcag gtggtttctg
     3781 gactaaaaaa gtgagaaaac gacaaggaaa ataactcaca aacttaaaaa ctgtctaata
     3841 tctaaattaa attttaaatg ttagacagtt tttatttatt attttgatta aaaaacatcg
     3901 acatatactc atcaatttgt tattatactt tatatactta tcaatttaat ttggagatat
     3961 tatggaaatt caacgtagaa ccactaattt tttacattta ggctcttttt ttgcaacttt
     4021 gacgatctta ttttttctat gttattcata ccttaagacc tcacatgcgc cttctagtgc
     4081 attaggctgg ttgcttttgg atcattctga tatcacagaa gccgaagaaa atccgttttt
     4141 tttgattttg tcttctcttt gcggcttact gttttctgtt tatttcggtg cctatttttt
     4201 tcatcgacat cctggtacgt taaacgaagc ttcaagcgta ttgattgcat ttaaaacgtt
     4261 ttctttttta gcaattcttt tttattcgtt taatcacaat acgcttttct tctttatcat
     4321 gaatggttta gtcgtgattt tatccatttt tacttatttt attttttcac tcatttttag
     4381 ccaattgaaa tgtccgctct tctttcgatt agttcctgtc acctgtttta tttacagctt
     4441 gttttccttt tttgtccttg ctttaatgcc actgaccaac ccttcgacga tccaagtcgc
     4501 aggaaatctg ttggatatgc tcttcatcgt cagtctaggt gtatttatgt ggcaaagaaa
     4561 aaacaaacaa aataaaaagc attttgaagc ctgactatta cgacttcata aatgcttttt
     4621 tagttttaaa cacttcaatc taaaagaata acccaagatt ggataaatta ttaatgactc
     4681 gccattcgtt tcttagtgat tctggattat caaatggact tcctaatcta atcagctagt
     4741 tcttcccata aaatgccata cttgactgaa tcataatatt gattatttaa aaatctaact
     4801 tttcttatca ctacttcctt tgtcattcca catttctcac catttttttg cattcctttg
     4861 tttccagacc atgtagtgaa tctaataggt ggtagatagg caaaatactc aaataattct
     4921 tttaaccata aaggaagaga aaggctcctc ctgcaagagt cagtataatt tttgcttact
     4981 gctctcttag gttttactca ctaaaactgt tccatattct ttgatataag aaaatgcatg
     5041 cggtataaat ttcttgctca gctcagaaaa gatttgtcgt ttaagaaagg ccatttcctc
     5101 cattgtcaat gaaggatcca atgttggtaa ttcaatctca aaattgtttt tttcgacaat
     5161 aggtaattct aatgtgaatg attcttcttc ctttatagta ttcgtcacaa gtagagactg
     5221 atggaaaata ctaggccggc gatttttata tttattctta cgtatcctgt caacgataac
     5281 ttctatttga tcaaaaaagt acgattcatt tacgtctcca ataagttcgg ctaattcttt
     5341 gaatgccttc gttacaggaa gctgattctt ctgtacaaaa aagtaattag ccaaagtaag
     5401 cgggaagtgt tctatagagg aaagtgtgat aaaacggtac ttctcatttc ctgcagttgt
     5461 cctcagcaat tctgtctctc ggctgtttct tactccataa aaatacgctt ctttccgttc
     5521 atcttcgaaa caatacgccc aaataacttc tcccatcgct aatttttgtt catcaacaga
     5581 gagtctcttc aattgttcag ctatggtttc agcagccgaa gtggcttgtt gcaagtaatc
     5641 tttaggatca aatattggaa agttcaaatg atatcttcgt tcttccctct tgattaatcc
     5701 tgcatcgatc aataaatcaa gaaaatggga aaatttattt tgcgaaaatt cttttttcaa
     5761 ttctcttaga atcgtttctt gttctcgatg ttcatataga taatcggtca atgaagaaaa
     5821 aagcggatca tcggtcagtt tttgataaaa gttttcctta ttttttgaat aatagttcat
     5881 atatgttctc ttttctatcc gttttatttt acttaaaagg cttttacgta aaaaggagga
     5941 agaacaagag tcataattga ctgttattct cctccatttc ttcggccttt agtatgttct
     6001 atgtttcttt ttatatcaat cgcttatcca ttcattttga atgcttcata ctaccatttg
     6061 attagctaaa tacagccgtc aattgaggta cgacttgttt tttacgtgaa acgacacctt
     6121 taagcaaagc acggttgttg gtcaaagttg tttggaaagc ttcttctact ttttcttttc
     6181 ctttaccagc aacaagtaat tctgaatcac tgtctaagat gtttgtgaca actaatacga
     6241 acagatcgta gccatttgct ttcatttcat cttccattgc tgcttctagc tctgcttgtc
     6301 gatccatcac ttcattaagg tccactgtat tgacttgagc gatacgaacg ttggaactgc
     6361 ccattgggaa actttttgca tccaaatcaa gaagaaccgc agctgattta tcacttagat
     6421 ttgttcctgc tttcaacatt tcaagtccat aagtgtttag atcgacttca gcgattgctg
     6481 caagttcttt tgcagctgct tcatcttcag gcgtacatgt tggtgatttg aacaacaatg
     6541 tatctgaaat gatagctgag agcatgattc ctgcgatttc tttaggaatt gttacttggt
     6601 tttctttata tagttttaaa ataatcgtac ttgtacatcc aactggttct gcacgatagt
     6661 acaatggatt agctgtttca aagtttgcaa tacgatggtg gtctactaca gctaaaatat
     6721 ttacttcgct gatatctgaa atactttgtt ggaattcatt atgatccaca agcatgacat
     6781 ctgcattttc tgctttagct gactctacta ctcgtggtgc agatagctga aagtggtcta
     6841 atgcgtattg tgtttcttca ttaggtgtac ctagagcaac tgcttctgtt tctttaccta
     6901 attcattttg caagtgtgaa aacgcaatgg ctgccccgat tgcatctgta tctggatttt
     6961 ggtgaccaaa aactaaaatt tttgacatgt cattcgctcc tttgtgattt ctcctattta
     7021 ataatagcaa aaaaacggaa agaagcaata cttatcttct ttccgatgcg acctatcgcg
     7081 ttaaatattt tgtatactct tctgtatgaa gcaatcgttt agcattttcg acacgttctt
     7141 ttgtaggtgg ttcgatccct tctaatggat atttcaaacc aagtgtttcc cacttgtatt
     7201 ttcccatcgt atgataagga agaatctcta ctctgtctac atttgacaag gtttgaataa
     7261 agttgtctag tcggatcaaa tattcatcat aatcacttcg ctcaggaact agtacatggc
     7321 gaatccaaac cggtttgttg atttttgaca aatattgagc catttctaaa atattttcat
     7381 tgtcccaatg cgttagtttt ttatgctctt cattatcgat gtgtttgatg tcaaataaca
     7441 gtaaatctgt atacttcatc aattcttgaa aacgtgaaaa gaaaggttct tcatacgtaa
     7501 aaggttttcc gcatgtatcc aatgtagtat gcatcttccg ctcttttgct tttttaaata
     7561 gttcgatcaa aaaatcgatc tgcaatagag gttctccacc actaacagta ataccacctt
     7621 tatctcccca gtaaggtctg aagcgttctg ctttatctaa aagttcatct gctgtgtatt
     7681 ctttccctcc gccaatattc caagtatctg gattgtgaca aaattggcaa cgcatgcggc
     7741 atccttgcat aaacacgaca aagcgcagtc caggtccgtc tactgaaccg aaagtttcga
     7801 tagaatgaac atagccgatt gttttttctt ccatttaagt tcacatcttt tctttgattt
     7861 cattttaagt acaagttagc tgtcgaagcg aagctttctg aacaaaattt ttcgcaattg
     7921 ccgcttcgtt atgtgtaaaa tttacaggga cggaacatac aagtcctgcc cctgtaaatc
     7981 gtttctttca aataagcata ctgattattc gattttgatg cgatggctat tcaacgtcct
     8041 ttcgacgcta agtcgaaaaa cttttttcac gaacttttta cattctgtcg tgtgaagttc
     8101 tcatcaagac atctaattgt tgttctcttg tcaagtcacg gaatttaact gcataaccag
     8161 atacccggat cgtcaagttc ggatatttca ttggattttc cattgcatca cgcaataaat
     8221 cattactgaa tacattgaca ttcaaatgat aagctccttt tgagaagtat ccatctaaga
     8281 cattacgcaa gttttcgatt cttgtatctt gttcttttcc aagacctgaa ggattgattg
     8341 tttgggtatt tgaaattcca tccaatgcac agccgtaatt cattttagct gttgagttca
     8401 ggctagctaa taacccactt tgttccgcat tgtaacttgg atttgctcct ggcgcaagtg
     8461 gttttccagc ttcacggcca tcaggagtat ttcctgtatt tttaccgtaa acaacatttg
     8521 aagtaatcgt caataatgaa gttgttggtt ttgagttgcg gtaagtttta tgacgtttga
     8581 tttgtcccat gaagtattcc aagatccatg aaactaattc atctgcacgg tcatcatcat
     8641 ttccgtattt agggaagtcg ccatccactt caaaatcaac tgctattcct ttttcattac
     8701 ggattgcttt tacttttgca tatttgatcg ctgacaagct gtctgccgca tgagagatcc
     8761 cagcaatacc tgtagcaaat gtgcgttgca agtcagaatc catcaaagcc agctgagctg
     8821 cttcataaga atatttatca tgcatataat gaatgatgtt caacgtattg acatatagtt
     8881 ctgctaacca atccatcata ttcatgaagt tatgtttcac ttcttcaaag tctagtattt
     8941 ctgatgtgat tggacgatat tctggtccaa cttgggcgcc agtcacttca tcgactccgc
     9001 cattgatggc atataatagt gttttagcca agttagcacg agcgccaaag aattgcatgt
     9061 cttttccgac aactgttcct gatacacagc aagcaatcgc acaatcatca gaaccccatt
     9121 gttcacgaag ctgattatca ttttcgaatt gaatcgaaga actttcgata gcgattctgc
     9181 tagcatatgt tctgaatcct tctggaaggt gttcagaata caaaacagtc aagtttggtt
     9241 ctggtgaagg tcccatattt gttaatgtat gaagcagacg gaaatcattc tttgtaacta
     9301 atgaacgacc atctaatccc atacctgcaa tggataaagt agcccagata gggaatcctg
     9361 agaataattg gttatattca ggtgtacgcg cgaatttgac catacgtaat ttcattacta
     9421 agtggtcgat caattcttgt gcttcttttt ctgtgattac tccattatcc aaatcacgtt
     9481 ggatatagat atcaaggaat gctgagatac gtccgattga catcgcagca ccattttgtg
     9541 atttgattgc tgctaaatat ccgaagtata gccattggac agcttctttt gcatttgcag
     9601 ctggtttaga aatatcatag ccatatttcg cagccatttc tttcatttgc gctaatgcac
     9661 gatattgttc agtgatttct tcacgtaaac gaatcacttc ttcactcatt gtatgataac
     9721 cagtcatatc atgatctttt ttcttttgtt ccatcaaata gtcgattcca tacaatgcca
     9781 cacgacgata gtcaccgatg atacgtccgc gaccgtaagc gtctggaagc cctgtaatga
     9841 ttttgttttt acgagcagca cgcatttcag cagtataagc gtcaaataca ccttgattat
     9901 gtgttttgcg ccagtcagtg aaaatatgtg tcaattcatc gtttggtgta tagccattgc
     9961 tttttagcgc attgttagcc atattgattc cgccgaacgg catgaatgct tgtttcaaag
    10021 gtttgtctgt ctgtagaccg acgatttttt ccaaatcttt atccaaatag cctgcatcgt
    10081 gtgaagtaat agttgatacg atttctgtgt ccatatcata gacaccgtta cgctcgaatt
    10141 ggatatcatt taacttttgc aattcatccc ataatttttc tgttgcttct gttgggcctt
    10201 ccaaaaagct gtcatcaccg cgatattcag tgtagttatt ttgaataaag tctctagtgt
    10261 ctacgccttc tttccatttg gtacctttaa atccattcca ttgctccatt gtatggacct
    10321 cctaaatttc tgttttatgt aacagttttt acgtgattag tataacacag caccctatct
    10381 ttgcaagggt tttcttgttt tttatttaac aatttttatg ttttatttac atatatttaa
    10441 atcaacgttg acataccaac atttaagcat tgtgaacact ttcatctttt tttgcaaacg
    10501 tataaggatt tattaaaaat gaatgaaaaa gagacgccac ctgtattaat acagatggcg
    10561 tctcttcatt aaaatactac aacagttacg actcgatgaa aatctcagga gcatcgataa
    10621 cttcgatgac ttctccacct tttttctcat ccatcgcaaa tgatccattc gatgttcgat
    10681 cacctatagg gaaatcagtt gggtcaatcg ttaactgctg ccctttttga ttcatcaagg
    10741 ttatttcttt tgatcttcct tccgacatat agataatacg gtgcggattt tttttcagct
    10801 cacgcatgac cgtcactccc cgcttcgcac gtgaagtttg gctgatttct tgagcaagca
    10861 tgcgtttaac agctcctcgc tgagtaacga ttacgatcgg cgtatcacct tctgctaaca
    10921 caagcagccc gttgataatc ctatctccat ctttcaaatt catagacttc actccagctg
    10981 cctttgctcc aacaacggga acatctgtta aaggatagcg caaacccatt ccttgatgcg
    11041 ttactaagaa aacatctaag cctggctgat cttctttcag atacacagca atcagttcat
    11101 cttcttccgc ctttagcttc atacagctga gcggacggct cttataggtg cgccatggtt
    11161 cgaactctgt cattcgtgtt tgcttgatca atccattctt gctgatgaat acaaatgttt
    11221 tctctgcttg aattttttga tatgggaaaa cagctaaaat cgattcgtct acagacagat
    11281 tggagattgt ttgagaaatg tgctcaccag catctttcca tttcaaatcg ggtagttcat
    11341 ggacagggcg gtagatcaca tttgctttat ttgtgatcag aagaacatgg tccaatgtac
    11401 tcaattcacc tgagtatagc aagtagtcgc cttctcgcat cccgatttct tctggtttgg
    11461 aagcactata agaacgaagg cttgttcgct tcacatagcc ttctcgcgtc acactgacaa
    11521 ttacgtcttc ttgtgccact aagacttgcg tatcaatttt gatttcttcg atttcgtctt
    11581 cgattgtcgt tagacgagcg gaagaataat tttttttcac ttctcgcaat tcttttttca
    11641 taacagagaa aagctctttg tcatttgaaa ggattttatt caactctgtg atttgcgcaa
    11701 tcaagctttc ggattccttt tgaagctctg tgatatccgt gttcgtcaaa cggtataatt
    11761 gcaatgtgac aattgcttct gcttgttctt ctgtaaattg aaagacgtcg actaaattct
    11821 ttttggcgtc ttttttgtct ttactttcac gaatcgttgc gattacttca tccagaatcg
    11881 ataaagcttt catcaatcct tctacgatat gctgtctttt ttgagctttg gctaaatcga
    11941 attgactacg ttttgttatg acttgctttc gatgctcgat ataacttctt aaaatgtctt
    12001 tcagtccaac ttgatgcgga gtcatatggt cgatagcgac catattgaaa ttgtaattga
    12061 tctgtagctc agtgtttttg aacaaataat tcaaaattcc ttgagcattg gcgtcttttt
    12121 tcaattcgac gacaatttgc agacctgttc tatcactttc atctcgtact tcagctattc
    12181 catcgatttt cttattcaaa cggatctcat ccattttttt caccaaggtc gctttattta
    12241 cttcatatgg gatttctgtg atcacgattt gctgtttatt acccttcatt gcctctatct
    12301 ttgttttcga acgaagaata acttttcctc gtcctgtttc ataagctttt ttgatttcat
    12361 cttttccttg caagatccct cctgttggaa agtcaggtcc aggaatatat tccatcaatt
    12421 tttcaaggct cgcttgtgga tgatcgatca tatagattgt gccatcgatc acttcagcta
    12481 agttatgagt cgggatttct gttgcatatc cagcagatat acctgtcgat ccattgacga
    12541 gaaggtttgg gtatcgagct ggaaggactg ttggttcttt ttctgtatca tcaaaattcc
    12601 acacgagatc gactgtttct ttctcgatat ctgctagcat ctctccactt aacttggata
    12661 atctggcttc agtataacgc atcgcagcag gtggatcacc gtccatactc ccgttattcc
    12721 cgtgcatttc gatcagtact tcccgcaact tccagtcttg gctcatgcgg accatcgctt
    12781 cataaatact gatgtcacca tgaggatgat aattccccat gatatttccg acagattttg
    12841 ctgatttacg gaatcctttg tcatacgtat tgccatcttt gttcatggca aataatatcc
    12901 ggcgttgcac tggcttcaat ccatcacgga tatcaggcag tgcacgttct tggataatat
    12961 atttggaata acgtccgaag cggtcaccca tcacttcttc taaagttaat tcttgtactt
    13021 cttgtcgatt ttccatcagg tgacctccta ttctacttca aataactgat tgttttcatt
    13081 tttatctgct cgttcttcat ctaataaatc attagaaact gaaggagaga tttcagtatc
    13141 ctctttttta tctaagatac tgccatcttc ttcgagcgta aattgcacgt gattttcgat
    13201 ccattttctt cgaggttcta ctttgtcacc catcagtgtg gttactcgac gttcagcttg
    13261 cgcagcatca tcgatacgga cgcggatcaa cgtacgggaa gtcggatcca tcgttgtttc
    13321 ccacaattgt tccgcattca tctctccaag tcctttatat cgctgcagca tatatccttt
    13381 gccgactttt ttaatcattg ctgccaattc gtcatctgtc cacgcatatt caatgacttg
    13441 cttctttcct tgtcctttgg atactttata taaaggcggt aacgcgatat atactttccc
    13501 agcttcaatc agcggtttca tataacgata aaagaaagta agcagtaata cttgaatatg
    13561 ggcgccgtct gtatccgcat cggtcatgat gattactttg tcgtaattac agtcttcgat
    13621 agaaaactca ggaccgactc ctgcaccaat cgtatagatc atcgtattga tttcttcatt
    13681 tttcaagata tcttgcattt tagctttttc ggtattgatg acttttcctc gtaaaggtag
    13741 aattgcttgg aactttctgt ctcttccttg tttagcagat ccgccagcag aatccccttc
    13801 gactaaatac aattcatttt tcttagggtt tcgggattgt gcgggtgtca attttcctga
    13861 aagaagagat tcgccttttt tacgcttctt cccatttcgg ctttcttctc tggctttacg
    13921 tgctgcttct ctcgcttctc tggctttgat tgctttacgg accaacatct ggctcatttc
    13981 gctattttcc tgcaaataaa agcccatttg ttcactgatg acgttatcta ctacggtacg
    14041 agcaactggc gtaccaagtt tttcttttgt ttgcccttca aattgcagaa gattttcagg
    14101 tacacggata gaaagtacag cagctaaccc ttctctgaaa tcactacctt ccagattctt
    14161 gtcccgttct ttcagcaaac caacttttct cgcatattca ttataagatt tcgtcatcgc
    14221 cgctttcata ccagcttcat gggttccccc atcttttgtc cgaacgttat tgacaaaaga
    14281 taaaacattt tctgaataac cgtcattgta ttgataagcg acctctactt cgataccttc
    14341 tttttctcct gaaaaataaa caactggtgt caatgtatct ttttcttcat tcagataatc
    14401 aacaaattct ttgattcctt cttcgtaatg aaaaatttcc ttgactggtt cttctccacg
    14461 aagatcagaa agagagattt taacgccttt tagaagaaag gcagattctc ttaaccgttc
    14521 ggctaaaatt tcatatgaaa atttcgttgt actgaatatt gtatcatctg gtaaaaaatg
    14581 gacagtcgtc ccgtttgctt tttttgtttt tcctatcttt ttcaaagtac cgtcaggttt
    14641 accgccgttt ttaaatttct gctgatactc tacgccatct ctaacgatcg ttacaattag
    14701 ccatttcgat aaggcattta ccacactggc tcctacaccg tgcagtccac cagaagtttt
    14761 gtaccctcct tgaccaaact ttccgccagc atgcagcaca gtaaaaatca cttcaactgt
    14821 tggtattccg gaagcatgca ttcctacagg catcccacgt ccgctatctg caacagttat
    14881 actattgtct tcatggatgg tcacatcaat ctcactgcca taacctgata atgcttcgtc
    14941 aactgcatta tcgacgattt catagactaa atgatgcaag cctcggctgt cagtggatcc
    15001 aatgtacatc cctggtcttt ttctaactgc ctctaatcct tccaatactt ggatggacga
    15061 gtcattgtat tcgttgttta ctttttttgc caaggcaaac gctccttcaa taatactttg
    15121 cggcaaaacc gcaccacgat tatcatacta gaaaaggtcg tgaaaaaaaa gagggtagac
    15181 gagaaatcaa ttttaattat ctcctaaccg ctttttcgac attcccctct atcgatcatc
    15241 tcttctttgt ggtcattttt atttttggtt ttctttcaac ttaccaagtt caatcttaat
    15301 acaacgatcc attacgatat ctgtacgtcc tgcttttctt agcatctcag ctgcttcttt
    15361 gttttcaatc cctaattgtg cccagaacac tttagcatca gttttcaaaa aatcacctgc
    15421 tatttcaggt aaaaattcac tccgtctaaa tacatctacg atatcgattg gatcatcgat
    15481 ttctgtcaaa ttcgcataga ctttctcgcc tagaatttct tcaccagcca ataatggatt
    15541 tactggtatg attttatatc cattgtcttg caatacctgt gcaaccatgt aacttgttcg
    15601 ttctggttta ttgctcaagc ctacgacggc aatatttttt gcttcttcca aatagttgaa
    15661 aatcgtttct tgtgttggat tttttacaga catttctgcc accccttgct tttcttttta
    15721 ttccatctat tatacttgta tctttcattt tttcaattgt tcctgttacc tattcatgct
    15781 aaaataaagg atagttttca acttgaaaga gggtaaaaaa tgaaattgat cttattactt
    15841 attttagcat atcttttagg ttctattcct tccggtgtct ggattggcaa attgtttttc
    15901 aaaaaagata tccgacaaca cgggagcggt aatactggaa cgaccaatac atttcgtgta
    15961 ttaggcaaaa cagccgggat tgttgtcctg cttatggata ttttaaaagg tacattggct
    16021 acttgtttgc cgatgatctt ccacctttct gggattaacc ctctttggtt tggggtatgc
    16081 gccattcttg gccatacatt tccgattttt gctgggttca aaggaggaaa agctgttgcg
    16141 acaagtgcag gaatgctttt aggatttaat cctgtatttt ttgtttattc ttgtatcatc
    16201 tttatcgtta gtttattttg tacaagtatg gtgagtttga gcagtatgat cagtgcagta
    16261 ttgatcactt tatctacgat cgtccttcct tatgtagctc ctgtgatttt ggctaagccc
    16321 aattggttac tgacgatcat tgccttttta gtttcttcct ttatctttta tcggcataaa
    16381 gacaatatca agcgtatcag aaaccatact gaaagtcgaa tcccgtttgg tttgaatagc
    16441 agcaagaaaa aatcctgata ctttttcatt ccaaagagat aaaacgggag tgtgacaaat
    16501 cgatgtcaca ctcccttatt acttacagga tagctttttt acacaccttg gtaaacggtg
    16561 ttccctgcta acttctcggt cacaacctct tacttcactg tgatggaata ttttgtcttg
    16621 aagatttcat gggcacctag ttgattgatc cctacttttt ccatcaagtt tccactactg
    16681 ttcaccgtgt cagcgattcc gcaccacggt tcgatacaaa cgaaaggcgc ttcttttgga
    16741 taaggagacc agatccctac ataaggcata tctttatagc ttaaagttac gctatgggaa
    16801 gatttttcac ttcgaatggc gtacgcattt agaccccttg tttcaaaaac caaggcatct
    16861 tgctcaaaaa gttcacgatt caaagccaga ttcgtatttg tctgacctaa cgtcttttga
    16921 tccatatcga tgaatgggcc tttcaatggt aggcgtaatc ttgatttcaa tggagaaaat
    16981 gccagataga aatcttcaaa tgacaatcct tcttcaagag gtacattgaa agcaggatgt
    17041 ccaccgatag aaaagaacat ttctttttca ccggtatttg ccacttgata atgaaccgtg
    17101 atgccgtctc ctcctaattc atagctgatc actaattcaa aatcaaatgg atacttttct
    17161 aatgtctccg gcgtactcct taagatgaaa ctagccattt cttgtccatg ttcaatgact
    17221 tcaaaatcca tatcccgagc aaatccgtgt tgccccattt gatatgtttt cccttgataa
    17281 acatactgat catcttttaa acgtccaaca aaagggaata aaaccggtgc atgtctgccc
    17341 caatacgctg gatcaccttg ccagatatac tctaaaccat gttcttttga tttcagactg
    17401 atcaattccg caccgtgttc agcgatttcg gcgatcagct ggtcattttg aataataaca
    17461 gtcattgcca ttcctccaac ttcgacttac tttttaaaat gggaaggctg gaacaaacat
    17521 ttctatctgc cccagtcccc ttacttctaa taactgtgtt ttgctaactc gtctttaaaa
    17581 cgggaattca atacttttaa ataagttccc ttcatgccta acgaacgaga ttcaatgatt
    17641 ccagctgatt ccaattttcg taaagcattg acgatcacag aacgagtgat gccgatacta
    17701 tcggcaatat tagaagcagt cagacgacct tcatctccat ccaatgcttc aaagatagct
    17761 tgtacagcct tcaattcgct ataagataat gtgttgattg ccatttggac agccgttgca
    17821 ctccgtacat ctgcttcgat gcttcgagac ttctgataaa ggatctgcat accgacaacc
    17881 gtagctccat attctgctaa gactagatcg tcatctgcaa acgattgttc catacgagat
    17941 aagataatcg tacccaaacg ttcgccagct ccaaagatcg gaacgacagt cgtcagacca
    18001 aatggatatt tctctcttga ctctaccgga aatgcagtaa tatcactagt aattgggata
    18061 ttagcttcag ttttcatcaa ctgtgccact gtatcagtat aagattcagg aaattgtttc
    18121 tcaacaaaca ttgttttgat tcgttcattg ttcacatcgt gacgttcatt ataacctagt
    18181 aatactccgt cacttcctat gatataggtg ttgctgtcaa gaataccacc tagtgtgaca
    18241 gccattttat cgtaaggcaa atctgcttgc atgtcaaaag tattcttttg ctgcaaaagt
    18301 gtgtttatct tgcgggtttt cgttagcaag tctgtcattg tcgtcctccg attttttata
    18361 aaatatagcg acttaaatct tcatttgccg caattgagcc aagtttgtca tccacatatg
    18421 cttctgtgat cgttatttct cccatttgca tatctggtgc ttcaaataat agatcttcta
    18481 gtagtttttc caaaatggta tgcagtctgc gtgcgccgat attatctgtg tcacggttga
    18541 catcgaaagc gatttgagcg atccgttcaa ttgcttcttt tgtaaacgta actgacacat
    18601 tttctgtacc gatcaacgcg acgtattgtt ttactaaagc attatttggt tcggtcagaa
    18661 tcttcacaaa atcttcagct gtcaaatcat ctaattctac ccgtatcggg aagcgtcctt
    18721 gaagttctgg aattagatca cttggtttag acaaatggaa agctcctgaa gcaatgaata
    18781 ggacgtgatc tgtttggatg atcccgtatt ttgtgtttac ttgtgaacct tcgacaattg
    18841 gcaatatgtc tctttggacg ccttctctgg atacttctcc agattgctgt gatttagaag
    18901 tgatcttatc gatttcatcg ataaaaataa ttccactttc ttccgctaat ttgattgcct
    18961 cactatggat atcagcatcc ttgactaatt ttgctgattc ttccttgatc aataactcta
    19021 aagcttcttt gactgttacc gtccgttcca cttttttctt cggtgtcaaa gcacctagag
    19081 tttcatttag gtcgatcccc atttgctcca atccattgtt catcattggt gcaggtttct
    19141 tttggtcttc aacttcaatg gttacttcac gactatccag taaacccttt tgtagttgtt
    19201 ccaagactgt acggcgattg attcgaatat cttctgtcac ttcttcttgc ggttcagcgg
    19261 tattttgctg actagcatta aaaagattca tcatttgttc aaattgatta gtggattgtt
    19321 tctgttcttt tttgatacca ggtaccaaga tttttaccaa acgtttttct gcattttttt
    19381 tagcacttgt gtatacacgg ctgtactgct ctttttctac gatttggatc gcattttcta
    19441 ctaaatcccg aaccattgat tctacatctc ttccgacata accgacttca gtaaattttg
    19501 ttgcttcgac tttgacaaat ggtgcattga cgatttttgc caaacgtcgt gcgatttctg
    19561 ttttaccgac ccctgtaggt ccgatcatca gcatattttt tggtgtaata tcttgttgca
    19621 ttttttcatc taactgcaaa cggcgatagc ggttacgcaa agcaacggca acagattttt
    19681 ttgctgcgtg ctgtcctacg atatattcat ctaattcact tacgatttgt cttggtgttt
    19741 gttttaattc atacatatct actcacctct tacagttctt ctacgataat attatgatta
    19801 gtaaagatac agatatctgc ggcaatattt aacgcatttt ctgcaatttc tttcgcactc
    19861 atgtcgtttt gagcatagtt tttcattgct cgggctgcag ccaacgcaaa attccctcct
    19921 gatccgatag ctaagatacc atcatctggt gtgataactt ctcctgtacc tgaaacaaga
    19981 agcatttctt tatcattcat tacgatcagc atcgcttcaa gtttttgcat agattgctgt
    20041 gtccgccatt cttgagctaa ttcgacagct gcgcgttgta gattcccgtt atattcgttt
    20101 agtttgcctt caaatttttc ttctaaagta aacgcatctg caacacttcc ggcgaaacct
    20161 acaacaactt ctccattgta gatacgtcgt actttctttg cagtgccttt catgacaaca
    20221 gattcaccca ttgttacttg gccatctcca gccatagcaa atttcccgtc tttttcgacg
    20281 gcacagatag tggttgaatg aaatggtgat tcgaccatgt ttgtttcctc ctaaatttgt
    20341 tcttttcctt atttttacgc tcttggatgg aaagaacgat agtttttttg taaactttct
    20401 tttgttacat gggcatagat ctgggtagtc gataaattgg catgtcctag caattcttgg
    20461 actgtccgca aatctgcccc attatttaat aaatgagtag caaatgtatg gcgcagcatg
    20521 tgaggatgga tatcactatc caaactgctt tttttgatca tttgattcaa gacatattca
    20581 attccagtag gcgtgattgg ttttccatga tgattcacaa aaacgatatc atgctcttcg
    20641 tgatattttg tcatcaatac ctggcggcct tccctgaaat attcttgcag agcatcttga
    20701 gcaaaagaac ctagaggagc atatctctct ttgttccctt taccatggat caacatgacc
    20761 tctgaatcaa aatcaatgtc tgataccgac aaactgctac actcacttaa acggatgcct
    20821 gacccgtaaa ggacttccag taaagcgcgg ttcctcaaat ccaaaggact atctcctgct
    20881 acgctgtcaa aaagtgcttg catttcattt tcataaaaaa aacgtggcag tctcaaattt
    20941 ttctttttga gatgaacgta agaaaatgga ttttctttta tcacttcttc tttgagaaga
    21001 tactgataaa acgaacgtag gctggctatc ttgcgactga tagaatttct gctatagcta
    21061 ggacgatgat cagctaatgc actcaaatag acacgaaccg taaaatattc cgccttcagc
    21121 agatcagcgt ctccagattc ctccaaaaaa tgtttgaaat ggagaatgtc ttcttcatat
    21181 gcttctttcg ttttttctga atatcctctt tcgacaatca aataacgcaa aaatttttgt
    21241 tcccacgtgg cttctgtcat tttctcacct tccttttgtc ccagagaata atttagcaca
    21301 gcgtttataa aaagacaaat aaattgtcag aatttaacca atatttctaa gtttgttcac
    21361 aattggcgca attcctcaca ttttgtttga gaaattctta agaaaaacgc aagaaaaaga
    21421 gcgagactat ccgtctcacc ctcttcatgg agtttcctta ctcatttggg attgcagttt
    21481 tttctaattc tgtttttatt gcctcattag cttttaaagc tcgttgtgca attgcttcat
    21541 atcttgcttt tttgtctcga atgcgctctg gcagttcagg aaacagacca tagttggcat
    21601 tcatcggttg gaaatgtttt ccttccgcat gagtaatgta gtaagccata cttccaatag
    21661 ctgtttcttg tggaaactcg actggttcca tttcttttgc taagcgtgcg gcgttgattc
    21721 cagcaagtag tccgctcgct gcactttcaa catagccttc tacaccagtc atctgacctg
    21781 caaagaacag atcttcgcgt ttcgttgatt ggtaagtcgg tttcagcaat tctggcgaat
    21841 tcataaagct gtttcggtgc atcacaccat agcgaacgaa ttcagcattt tctaacccag
    21901 gaatcatccg gaaaacacgt ttttgctcgc cccatttcaa gtgagtctga aagcccacga
    21961 tgttgtataa agaagctgca gcattgtctt gacgcagctg gataacagca taaggacgtt
    22021 tgccagtttt aggatcttcc aatccaactg gtttcatagg tccgaaaagc atcgttttga
    22081 atcctcgctg tgccattact tcaattggca tacatccttc aaaatatttt tctttctcaa
    22141 aagatttaag ttctgccact tcggcattga ttagtgcctc atggaaagct ttgaattcct
    22201 cttctgtcat cggacagttc aagtaagctg cttctccttt gtcatagcgg gacttcaaat
    22261 atactttatc catatcgatc gtagctttgt ctacgattgg tgcagctgca tcatagaaat
    22321 aaaaaccatc tgaaccattg aatgctttga tgctttctgc taatggatga gaagtcaaag
    22381 gtcctgttgc tactacggtg attccttccg gtatttcagt aatttcttcg tttatcactg
    22441 ttactaatgg atgctctttg acttttcggg taattgtttc agaaaaagaa tcgcggtcaa
    22501 cagctaaggc accaccagct ggcactgctg tttcatctgc cgaagtgatt actaccgaat
    22561 tcaatcggcg catttcttct ttcaataacc ctactgcatt agccaaactg tttcctctta
    22621 atgaattgga acatactagt tcagcaaaat tacttgtgtg atgcgcctca gtcgatttga
    22681 ctggacgcat ttcatataaa cggacaggga ctcctgcttg tgcgatctgc caagcagctt
    22741 cacttccggc aagccctgct ccaacaacat ttaccatttt caatgaaaga acctcttttc
    22801 tattattttt ggatgtcttc ttcgtaatct ccatttacac agactacttg tttaccgcct
    22861 ttgactttct tctcgaccag atatccgtca cattttggac aatttctgcc cactggttta
    22921 tcccaagaag tgaattcaca ttctgggaaa cgggaacaac catagaatag tctgtttttc
    22981 ttagattttc gttcaatgac ttgtccttca tgacatactg ggcaagtcac accgatttct
    23041 tttacgattg gtttcgtatt ccgacaatca ggaaaattac tgcaagcata aaacttccca
    23101 tatttcccta attttatcac catcggatgt ccacagacat cacaatcgaa tcctgctggt
    23161 tcatctttga tttgaatttt ttcgattttt tcttccgcat tcgtcagttc tttttcaaac
    23221 ggtttataga aacgatcaac cactttgacc cacgcttccg tgccttcttc aattttatcc
    23281 aaatctcctt ccatttctgc tgtgaaatgg atatcgacga tttgagggaa gaattcacag
    23341 attaatgaat tgacaatttc tcctagttca gtcggctcaa atcttttttg tgtcaatttt
    23401 acatagtagc gtctttggat cgtatcaagt gttggtgcat aagtggaagg tcgccctacc
    23461 ccgttttctt ctagtgcacg aatcaacgta gcctcactaa aacgtgctgg tggttgagta
    23521 aaatgttgtt ttggttcaat atcaacagat tggactacat cgccttcttc aagttctgga
    23581 agaatatttt ctttctcttc tttgccgtca tctctacctt ccacatacac ttgcatgaat
    23641 cctttgaatt tgatttttga accgttcgca atgaagatga caccattttg ttctaatgtc
    23701 actttcattg tatctaaaat agctggtgtc atctgactag ccacaaaacg tgaccagatc
    23761 aatgtataaa gtttcaattg atctttatct aaatattgtt tgagttcatc tggtgtacga
    23821 agcacactcg ttggacgcac agcttcatgg gcatcttgtg cgccttgagc atttttgatc
    23881 tttcggtgat catgtctaga aaattctttt ccatacgtat cttcaataaa tgctgcggcc
    23941 tctgcttttg ctgtatcggc aatccgggta gaatcagtac gcatgtatgt aatcaagccg
    24001 actgttcctt gtttacccaa agtgattcct tcatataatt gctgtgctac catcatggtt
    24061 tttcttgttc gaaagtttaa cttacgggca gcttcttgtt gcaggctact tgtagtgaaa
    24121 ggattcgcag ggtttctttt gcgctctttc ttttctactt ttttaacttc gaagtctgtt
    24181 ccatcaattc tagcagtgac ttcttttaca ctttgtgcat ctggtaattt ctttttctta
    24241 ccgtcgattc cccagaaatt tgctttgaat ttcttgcgag cttttttaaa attaccgtcg
    24301 atgctccaat attcttctgg aacaaattct cggatctctt tttcacggtc aataataatt
    24361 tttaaagcga cagattggac acgtcctgca cttaaccctt tcttgacctt acgccagaga
    24421 ataggactga tcgaataacc aacgattctg tctaaggtac gacgagcttg ctgtgcatct
    24481 actaaatcta catcgatcgt tcttggttcc ttaaaagctg ctttgactgc gtcttttgtg
    24541 atttcattga agaccacccg atttttttct ttcagatcca acccaagtag gtacgctaaa
    24601 tgccaagcaa tcgcttctcc ttctctatcc gggtcacttg ccaagtaaac tttttgagct
    24661 ttttttgctg cggctttcag gcttttgatg acatcgcctt ttccgcgtat agaaatataa
    24721 tggggttcat aattgttttc aaaatcgata cccattttac ttttcggtaa atctcttata
    24781 tggccaacac tggccattac tttatagttt cgtccaagat atttctcaat tgtcttggct
    24841 tttgcaggtg attctacaat cactaaatat ttatacgcca ttactgaatg gactcctttc
    24901 cattgtcgtg ttcctactat taaaagagga ttcatacaaa tcgtagctaa tcatatccat
    24961 tcagaaaacg tttgtcaaga aaagcaaatg aaaagtatgg tgttattctt taaattctgc
    25021 caagatatct tcacccgaaa ccgtgcaaac tgcaccgtct tggatcaaat gatgacatcc
    25081 ggtagaatgt tctgttaaaa tatttcctgg aacagaaaaa actgttcttc caagatccaa
    25141 tgcttgctgt gctgttatca gagagccgct ttttttcttg gcttcaaaga cacatgttcc
    25201 tttagacaaa gctgcgataa tccgatttct cattggaaaa caatgctttt tcgggccgga
    25261 ttgaggagga tattcactca aaagaagatg atcatttgca atcacctctt gcaagtcacg
    25321 attttcagca ggatagactt gctctatccc tgttccaatg actgcgattg tccgtccacc
    25381 atgagagatt gctgcttggt gaccgtatgt atctatccct ttcgcgcagc cactgacgat
    25441 cacatatcca gcttctatca ctggttttag taaataataa actgtttttt tggctaaagc
    25501 agaagtgtgt cttccaccaa caacgcttaa catgttacaa gaaagtaaag atagatcacc
    25561 attataaaac agtacaattg gcggataggt caagtgaagc agctgagggg gatatttggg
    25621 atcaaagcaa gtgataaacg actgtttcga ttggattttg ccccagttag cgtgaatttc
    25681 ctgccaactt ttgcgaaaca gctcccggtg tttggttatt ttacctatct taatgatgtc
    25741 ttcaaatccg atttctttat aaggatactg catcatccag ttagcaagtg cccattttcc
    25801 ttgactactg attccttttg tataagcaag tttcaataaa ttttcttcta tttgatacat
    25861 cataaaaccg cctttcgtct ctctttctaa taagatacgt aaaagcggct gttttttatt
    25921 gtaaatattt ttttacaggt gcaaaacttt tacgatggat tggcgtaatt ccttgtcgtt
    25981 caagtccttc taagtgttcc tttgttccat aacctgcatt atgcgagaaa ccatatccag
    26041 gataaagcac gtcatattct ttcatcaatt ggtctcgata tactttagca atgatacttg
    26101 cagcagcaat cgacacagat cgtgcatccc ctttgattat tttttcctgc tgaataggca
    26161 aatccaaggt catcgcatcg atcaacaaat agcttggtac aatggacaat ttttctactg
    26221 cttggatcat cgctaattta cttgcttcat aaatattcac atcatcgatt gtctgctgat
    26281 caacgatccc gatcccgatt gccagggctt tttcttgaac ttgttgataa agaagttctc
    26341 gctttttctc tgataattgt ttggagtcat tcagccctaa tatgatctca tttttcggca
    26401 agataactgc agctgccacg acaggtccag ctaatggtcc tcttcctact tcatcaatgc
    26461 cggcaatcca ttggtgacct tgtgcgtacc cttgcttttc aaagcaaaac atttcttcat
    26521 atttttcctt aattaatgct tcctttttca atcgtcgttc aaaagaagcc agtgcttgct
    26581 gcacgccttt gcgtggatca tttcttaatg attctagtgt aggatcatct attctctgta
    26641 tgcttttcaa tcgttctttg atactttgga tggattcagt catcactttt ttgctccatt
    26701 tcttcgcaac gatccagtgt gtatcgtcca agttttcctt gacgaatatc atgcacgact
    26761 aattcgctgc cacgatcata gtcttcttta tacccacgtt ttgctgtaat atccatcagt
    26821 aattccggtg ccaatttttc ttcttgctcg attgataaac cataacgatc gctgattctt
    26881 cctgaataat atcgggcaaa aaagtctaat ccgtaaatcg ctaaatcatc taaatggaca
    26941 agctggtctt tgatcgcgcc tgtcagcgct agttttttgc cgatttcttc atcttcaaat
    27001 ttgggccata aaatccctgg tgtgtctaac aattccaact cattacctaa acgaagccac
    27061 tgctgacctt ttgttacgcc aggcttattt cctgtctgtg caattttctt gcctactagt
    27121 cgattcatca acgtagattt cccaacattt ggaataccta agaccattgc ccgaatagcg
    27181 cgaggcttga tcccccgagc agcatctctt gcccgttttt ctttcaatgc ttctttagcc
    27241 gcaggtacta gacctttaat tcctttactt tcttgtgccg tgatagctaa tgcaagatgg
    27301 ccttgtttac gaaaatattc ttgccacagc tttgtctgtt gtggatctgc cagatccgct
    27361 ttattcaata aaacaaggcg aggtttttgc tgaatgatct gatctagcat tggattacga
    27421 gaagataaag gtaatcgtgc gtctaccaat tcaaatacga tgtccacata tttcattttt
    27481 tcagaaactt ctcgtcgggc ttttgccata tgtcccggaa accattggat ggtcattgtt
    27541 ctctccattc ctttcatacc ggaatattcc ccggctttac ttgttttttt ctttttcaat
    27601 ataaattcac ttttttatta taaagaaatt ccatctatta gttaagtgaa agtgaaccag
    27661 tcctcattct atcacatctt cacttttttg atgatgcaaa tttttatttt ggtttctata
    27721 tatgaatcta aatttttgac agcgtttcct aaataagaga tgatagacga acaaaacaga
    27781 aaggagacga aacaaatgaa tagtatggtt tttgttaatt ttcctgttgc tgatgtcaat
    27841 cgttccgttg ctttttatga aaagctgggt tttaaaaaaa atgaagaatt ttctactgaa
    27901 gaagctggtt caatggtatg ggatgacact ttttggataa tgcttctctc tcatgatttt
    27961 tataaaaaat ttataaaaga taaagaaata gctgataata aaaaaacaag tggtgctttg
    28021 atttcgttta gtgttgaaaa tgcggatgaa gtaaaaaaat ttgctcaaat tgcaaaagaa
    28081 aatggtggta ctttccacga agtagatatg ggaatgcctg aagaagaaat gttttcatta
    28141 gaagtccaag atctagacgg aaatacatta gaacccgtat ggatgaagat gtaagcagat
    28201 aactaatctg acgtttttta taacaaaaaa acaaaggcgg atgatacgat aagtattctc
    28261 cgcctctatt ttttttattc gtacaatctc ttttttacag tttataaaag cagtcttctc
    28321 accctttttt taattgttag atgccagtgt atgtaaatca ccaatgtgtc atgacacatt
    28381 ggtgatttta tggtatcctt ttattattaa ctgacaaagg agagaatatt ttgaacactt
    28441 tgattgcaaa agacgttttt agattgcccg aaatgaatgc aagattgttg tcgacctctg
    28501 ctcttttatt agcccttgat gtcatcttat acaagatagc gattggtcct gcttattttc
    28561 aaatcacatt cggattttta agtatggcgc ttatgggtta tctatatgga ccgatctggg
    28621 ctggtttgct tgaaatgttg tcgaacactt taaatttcac gatatttggt tcaggaacgt
    28681 ttcaagctgc ttttcttatc acggctttcc ttggtggatt tattaatggc ttatttttat
    28741 atcataaaaa gataaacctg ccttccgtca tcgctgccca attattgatt atgatcccag
    28801 tcagcctttt tatcaattct tggctgatgt ctgttttgtt tggtgcagat ttcaaagtaa
    28861 tttttctagg tagattcatc aggaatatga tccaaatccc tattcaaata atggttgttt
    28921 attcatttat caaaacttta gaggctagaa aagtgtttaa acgagctgtt tgattttacg
    28981 tactaaggta gtaaactgat tttggattaa acaataaagc gtgcagtctc aacagactgc
    29041 acgctttatt gtttctcact ggatccattt tatttcatca agtggataat agacaaatct
    29101 tgctttacct agaatatcag ttttattgat cgtgccaaac gaacgactat cttttgaaac
    29161 tcttcgatta tctccaagta caaaataatt atcttccgga agcttggatt gtcccgttag
    29221 ctcttgtagt gtaaagtccg tcgtatagga tgcatttgca tgatctgatt tcaggttttt
    29281 agttagataa ggttccttaa ctactttccc attgatcttc aattgatcat tttggtagct
    29341 gacattttct cctggaagac cgattactcg tttgatataa atcgtgccat ctgctaattg
    29401 aaaaacaaca atatcaaatc gtctgatctc cgaaaatttt tccatcacaa ccatatcacc
    29461 ttgcttcaac acgttttcca ttgaattgcc ttccactggt accgggatga ataaaaatcc
    29521 ccgcaacatg aacgcaagga atacagaaac aagtaagtat ttgaatacaa gccagaagcg
    29581 attaatatag cgttgttttt ttgtcaatct ctatcaatcc atccctcttt ttttcattct
    29641 ttctttatta taagaaaaat atcaaaaaaa tgcgactaga tagctgaaat aaaaataatt
    29701 ctttcaatta ggctattgga caaaaaactt ttttgatcat aaagtacaaa actatctctt
    29761 atatgtcatg attattttct aaatattttt cccatgcttc gtcaaagata tccatggacg
    29821 gcaaatagtc gacattcagt tccagataat tagaaatttc ttcgtagcta gagctgtgct
    29881 taggaaattg gatgtcttta ccggcttctg tagcgaaaac agataaagca tcatgtgtgc
    29941 caccgcctcg cagtgtcatt aaataatgaa aaaagcttcg attcaccctt cgttcctcca
    30001 attttcttcg ataaaggcag cccggcgtgc atggtttctt ttaaaatttt caggatgctt
    30061 tttataatac tcttgatggt cttcttctgc tagataaaat ggttgggctg gttcgatttt
    30121 tgtaacgatt ggctgttcga aacgtccact ttcagctaac tgtttcttac ttttttcagc
    30181 aatgatccgc tgttcttctg tcaaataaaa gatcaccggg cgataattgt caccgcgatc
    30241 ctcgaattgt ccgaaagcgt cagttggatc agtttgctgc cagtaaagtt ctactaattg
    30301 ttcatatgaa atcaatgttg gatcatagat aatctccact gcttctgtat gtccagtcgt
    30361 ttggcttttt acttgttcat aagtcggatc tggtacatgc cctccagtat atcctgacat
    30421 cactgtatgg ataccaggaa gcgtgtcgaa aggctggatc atacaccaga agcagcctcc
    30481 tgcaaatatt gctcgttcca ttatgattcc ccctctaata ctactcatcc gatgattttt
    30541 tattttcttt tgtcttgctt tcagtactgc tggtgctagt tttgttggta tttgagttgt
    30601 ttgaagcatc agaggtatct tttggcaaat atagactaaa ccgaatgtca tcatttacta
    30661 agtcgatttt atctgcttta atatacatcc cgttttgcat tcggaattga tccaacctaa
    30721 gcatgatagt cagatcatct gtattgactt cgatccaatc tggtaattta tagtttctct
    30781 tgatcatatt catcacatct ttgattggca aactcaatgt tccgatagaa agacttctag
    30841 cttttagttg gacatttccg ttttccatca catacggatc aaaatataaa tagaaatcga
    30901 taggaaaacc tagtacttcg aaagtaccat tgagtagtgc ttcattttcc aaataaaatt
    30961 tatattcaat atcactatct ttttgatact cactaaggaa aaagctgatg atttgattga
    31021 ctttttcttt atttgaattg atcgttaaga caggatctcc ttgtctttcc gtaagagctg
    31081 gtatttctgg gagttctggt tcccgattag caaaaatacg gctgcctaaa aatacggtcg
    31141 aaccaagtac taccccaaga aggataagga aggcgaattt ccagtaattt gttttctttg
    31201 ttttgtttga agatacgtca gatcttgttt tcatgtttat tgatcatcct ccttctctaa
    31261 ccattggttt tgtgtctcga tcagctgatc tctcactgca tttgccatca attgataacc
    31321 taaattattt ggatggaaat gatcttcttc atagagcaca ttatttttga tgccatttag
    31381 attttctgtc gtgctagttg tttgatctgt tccttcttct gtgacaccta cttcctgatt
    31441 caatccttga taaagcaaat cattgattgg gataaagtag cagttcggca ttttttttgt
    31501 caccgtttct gttcctttgt tccaattgtt aacaattgtc tgcatgtcag tgatctcagg
    31561 aaagttcaaa taaaatggat tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag
    31621 ggatcgaaat tcttccaata gttctgtgac attttcctga tagtttttca gtggcttttt
    31681 aaaggaattg accgttaacc cgaataaatt attttggata actttcatca aatcattacc
    31741 gccaacagtc agtgtgataa aatcagctgt tttgatattg tttcgaattt cttcttttgt
    31801 ttttactcgc tttaagatct ggtcacttcg ttcaccattt acaccatagt tttctgtttc
    31861 aaccgcggtc aaattataac ggtcttttag atcatttgcg acaaggggaa cgaatcctcc
    31921 ttgcttggtt tgatctccta ctccttcagt cagcgaatct ccgatagcta tgtagtgaag
    31981 tttttctttt ttatttggat cttctctcac agtagttact ggtttcatta gtgggccagc
    32041 tttcggtata gaagcagcta aacctgcata gatgatgatc gcaattaata atgggaacaa
    32101 aacgaggatc aaatgtcgtt tcgttaattt cattttttca cctcttgtat tcgattggtt
    32161 ctaatgttat caaaaatttt atcaaaaagg tagtctttat gaaaaaagaa aacaattatt
    32221 ttcctaattt tataaagaaa aagagaataa gctgcaagcc tttcaggctg agggcttatc
    32281 ctcttttcga ctaatctaca taatacatga ttgcaaacgc gtctttacca gtatgcgtag
    32341 cgataaccgg attcgtatgc agtacaggga tatgcatgtc cgggaataat tcttgcaaac
    32401 cctctttgaa ttcttgcgcc aattcttttg catccgcatg tgaaataccg atttgtcgca
    32461 catttggcaa ttctttcagt tcatttttca aattattaaa ccatttatcg aacgttttct
    32521 ttcctcgccc cttagttaca gggattaatt cagaatgggc gaaattcatc accacgcgta
    32581 tgttcaaaac actagacagc aaaccagttg ttcggctgat tcttccacct tttaccaagt
    32641 tatctagtgt ggatattccg atgtataatt ttgtattgtc ttgtactttt ttgacttctt
    32701 caaggatttt ctctactgtt ttaccttctt ttgctaattc agctgctcga attacctgaa
    32761 aagataatcc ttggtcagtg gtatcactgt cgataaccgt tactttcgtt tttgttagat
    32821 tactggcttg tcttgctgct tctactgttc cactcaagcc ttttgtcata tggatcgaaa
    32881 tcacttcact gccgtcttct cctagctcat catacatcga gatgaattct ccaattggtg
    32941 gttggcttgt tttgggcaga ttttttgctt tcgccatcat ttccataaat ctttcgccgt
    33001 ctagttggtc atcatcggga tatactgttc cgtcgatcat gatggatagc ggcatgacac
    33061 gaatgcctaa agcatcgcgt acattttttt ccatcgtaca tgaagaatct gttacgattt
    33121 ttacatttgt cattattttc tctctctctt tctttaacaa gatgccttat tcatgttaaa
    33181 ctacatgtaa gacaatttct atttttagta taacagacaa ataatgattt ttcattaatt
    33241 acacctagta aaggagccgt tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata
    33301 ttaaatgaag tgtttaatgc agtcacccat ggaattggga ctggattgag tatcgcggga
    33361 ctagtggttc ttttagtaaa aggtgcccac ttgcattcct ccctccatat tgtctcttat
    33421 gctatctatg gatcgatgat gattctttta tttttaacgt ctactttgtt tcattcattg
    33481 atttttacaa aagcaaaaaa ggtgttccaa gtattcgatc atagttcgat ttttcttttg
    33541 attgcaggaa gttatactcc tttttgtttg ctgagtatcc gaggatggct aggttggact
    33601 ttatttgtat taatctggtt gtttgcgatt cttggcatta tatataaagc gttgacgttg
    33661 cataaacggg agagagtatc aaaagtatct acgatcatct atatttttat ggggtggctc
    33721 tgtgtcgtgg ctgctgttcc tctttatcac tctcttggaa tgactggtgt tattttgatg
    33781 gtagcaggcg ggctttctta cacgataggc gcattctttt atagcttgaa ttctatccgc
    33841 tatatgcatg ttgtttggca tttgtttgtt atgttaggtg caggttttat gtacttctct
    33901 gttttattct ttacttaata aaaaaaataa agctatggat gggacagctc taaataacag
    33961 ctagtctatc catagctttt ttatatcgtc aattcgcctg tcccgctttt tacaggattc
    34021 tcattacgct gatacgtttc aaattgatgc ggataacgat ttttttcatc catttctcct
    34081 tgactagtat tgatcagtga ccaatcagac cagtcaacag gtggaaaata agcatctcca
    34141 tcgaatacat gatggatgac tgtacggtat aatatcgtac aaaatggcag aaattcttga
    34201 taaacagctg accctccagc gatgaaaaag ataccgtctg tttctttttc cagagcaagc
    34261 acgtcttcca cactatgcat gacatgcacg ccttctgctt tataggaaga atctctcgtc
    34321 aaaacaatcg tctgacgatt cggcaacgga cggctaccca tcccttcgaa tgtttttcgt
    34381 cccatcacta gtgtatttgc ttctgtcatt tgtttgaaga atttcagatc atttggcaaa
    34441 cgccaaggaa gacggtcttc ttttccaatc aatccgtttt catcctgtgc ccaaattgca
    34501 gctaacattt tctcatcctt tctgattcta aacagcgata ggagctttga tcgccggatg
    34561 tgggtcatat ccttctaatt ggatatcttc catctcaaaa tcaaatactg actgcttttc
    34621 ctgattcaaa actaaagtcg gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg
    34681 ttcgatatga ttgatataaa gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc
    34741 taaacctgtt tcatgtgcaa ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg
    34801 aacgcctaga aacacatccc cactgcgttg atacaattgg cagcttagcc gtccctcttg
    34861 aacataaaat tgaaacatcg tatgacaagg aggcaaagcc atgctaggaa catcttccgg
    34921 attccatgca gaaacgatca atcttctaga atcaggagtc tttttgattg cttcgatcac
    34981 ttgtttgatt tgatcgataa agccaccgtc ttttgtttcc cagtgacgcc attgatagcc
    35041 gtagatgtgt ccaagatcgc cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc
    35101 gcaaaaaagc ttgctctgct ttttatattc ttcattgaat tcaggatctg tcaaacatct
    35161 atggccaaaa tcagtcatat ctggtccatt atagtctgga cttttgatgt agcgttcaaa
    35221 tgcccattca tcccaaatat ggtttttatt ttgtaataga tagcggatat tcgtatctcc
    35281 ttttaaaaac caaagcaatt cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa
    35341 caaaggaaac cctttttgca gatcaaaccg catttggtat ccgaataagc ttaatgtgcc
    35401 tgtgcctgtt ctgtcttctt tacgatgccc ttcttccaac aattttcttc ctaagtcaag
    35461 atacgcttgt tccatcct
//
LOCUS       DAYYNQ010000016        34962 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000016 DAYYNQ010000000
VERSION     DAYYNQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.016"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHBJ1_000747"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHBJ1_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM9429714.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHBJ1_000748"
     CDS             complement(2020..2922)
                     /locus_tag="ACHBJ1_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9429715.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHBJ1_000749"
     CDS             complement(2919..3611)
                     /locus_tag="ACHBJ1_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM9429716.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHBJ1_000750"
     CDS             complement(3654..4295)
                     /locus_tag="ACHBJ1_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM9429717.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHBJ1_000751"
     CDS             complement(4333..4647)
                     /locus_tag="ACHBJ1_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429718.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBJ1_000752"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHBJ1_000752"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM9429719.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBJ1_000753"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHBJ1_000753"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM9429720.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHBJ1_000754"
     CDS             complement(5984..6916)
                     /locus_tag="ACHBJ1_000754"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM9429721.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHBJ1_000755"
     CDS             complement(6913..7689)
                     /locus_tag="ACHBJ1_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM9429722.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBJ1_000756"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHBJ1_000756"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM9429723.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHBJ1_000757"
     CDS             complement(10933..12009)
                     /locus_tag="ACHBJ1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM9429724.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHBJ1_000758"
     CDS             complement(12014..13294)
                     /locus_tag="ACHBJ1_000758"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM9429725.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHBJ1_000759"
     CDS             complement(13313..14239)
                     /locus_tag="ACHBJ1_000759"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM9429726.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHBJ1_000760"
     CDS             complement(14252..15535)
                     /locus_tag="ACHBJ1_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM9429727.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBJ1_000761"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHBJ1_000761"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM9429728.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHBJ1_000762"
     CDS             complement(16323..17234)
                     /locus_tag="ACHBJ1_000762"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9429729.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBJ1_000763"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHBJ1_000763"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM9429730.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHBJ1_000764"
     CDS             complement(17700..18398)
                     /locus_tag="ACHBJ1_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM9429731.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHBJ1_000765"
     CDS             complement(18385..18771)
                     /locus_tag="ACHBJ1_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429732.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHBJ1_000766"
     CDS             complement(18918..20588)
                     /locus_tag="ACHBJ1_000766"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM9429733.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHBJ1_000767"
     CDS             20771..21004
                     /locus_tag="ACHBJ1_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9429734.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHBJ1_000768"
     CDS             complement(21103..21534)
                     /locus_tag="ACHBJ1_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM9429735.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHBJ1_000769"
     CDS             21613..22026
                     /locus_tag="ACHBJ1_000769"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM9429736.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHBJ1_000770"
     CDS             complement(22064..23212)
                     /locus_tag="ACHBJ1_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9429737.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHBJ1_000771"
     CDS             complement(23334..23930)
                     /locus_tag="ACHBJ1_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9429738.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBJ1_000772"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHBJ1_000772"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM9429739.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHBJ1_000773"
     CDS             27160..27591
                     /locus_tag="ACHBJ1_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429740.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHBJ1_000774"
     CDS             complement(27636..28985)
                     /locus_tag="ACHBJ1_000774"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM9429741.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHBJ1_000775"
     CDS             complement(29055..30218)
                     /locus_tag="ACHBJ1_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9429742.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHBJ1_000776"
     CDS             30347..30670
                     /locus_tag="ACHBJ1_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429743.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHBJ1_000777"
     CDS             complement(30853..32202)
                     /locus_tag="ACHBJ1_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9429744.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHBJ1_000778"
     CDS             complement(32271..33224)
                     /locus_tag="ACHBJ1_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM9429745.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHBJ1_000779"
     CDS             complement(33326..34516)
                     /locus_tag="ACHBJ1_000779"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM9429746.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACHBJ1_000780"
     CDS             complement(34716..>34962)
                     /locus_tag="ACHBJ1_000780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429747.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAYYNQ010000017        34510 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000017 DAYYNQ010000000
VERSION     DAYYNQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.017"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACHBJ1_000781"
     CDS             212..604
                     /locus_tag="ACHBJ1_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9429748.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACHBJ1_000782"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACHBJ1_000782"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM9429749.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACHBJ1_000783"
     CDS             2698..2943
                     /locus_tag="ACHBJ1_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429750.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACHBJ1_000784"
     CDS             3165..4775
                     /locus_tag="ACHBJ1_000784"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM9429751.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACHBJ1_000785"
     CDS             complement(4752..4964)
                     /locus_tag="ACHBJ1_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429752.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACHBJ1_000786"
     CDS             5067..5936
                     /locus_tag="ACHBJ1_000786"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM9429753.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACHBJ1_000787"
     CDS             6101..7372
                     /locus_tag="ACHBJ1_000787"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9429754.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACHBJ1_000788"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACHBJ1_000788"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM9429755.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACHBJ1_000789"
     CDS             8763..9026
                     /locus_tag="ACHBJ1_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM9429756.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACHBJ1_000790"
     CDS             9471..9920
                     /locus_tag="ACHBJ1_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9429757.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACHBJ1_000791"
     CDS             9977..11395
                     /locus_tag="ACHBJ1_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM9429758.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACHBJ1_000792"
     CDS             11395..12255
                     /locus_tag="ACHBJ1_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM9429759.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACHBJ1_000793"
     CDS             12429..13202
                     /locus_tag="ACHBJ1_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9429760.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACHBJ1_000794"
     CDS             13349..13714
                     /locus_tag="ACHBJ1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM9429761.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACHBJ1_000795"
     CDS             complement(13862..15070)
                     /locus_tag="ACHBJ1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429762.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACHBJ1_000796"
     CDS             complement(15089..15367)
                     /locus_tag="ACHBJ1_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429763.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACHBJ1_000797"
     CDS             15560..16216
                     /locus_tag="ACHBJ1_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9429764.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACHBJ1_000798"
     CDS             complement(16658..17455)
                     /locus_tag="ACHBJ1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM9429765.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACHBJ1_000799"
     CDS             complement(17452..18636)
                     /locus_tag="ACHBJ1_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM9429766.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACHBJ1_000800"
     CDS             complement(18661..19908)
                     /locus_tag="ACHBJ1_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9429767.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHBJ1_000801"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACHBJ1_000801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM9429768.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACHBJ1_000802"
     CDS             21888..22202
                     /locus_tag="ACHBJ1_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM9429769.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACHBJ1_000803"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACHBJ1_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM9429770.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACHBJ1_000804"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACHBJ1_000804"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM9429771.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACHBJ1_000805"
     CDS             23546..23875
                     /locus_tag="ACHBJ1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM9429772.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACHBJ1_000806"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACHBJ1_000806"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM9429773.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACHBJ1_000807"
     CDS             24863..25687
                     /locus_tag="ACHBJ1_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM9429774.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACHBJ1_000808"
     CDS             25680..26027
                     /locus_tag="ACHBJ1_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM9429775.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHBJ1_000809"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACHBJ1_000809"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM9429776.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACHBJ1_000810"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACHBJ1_000810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM9429777.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHBJ1_000811"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACHBJ1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM9429778.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHBJ1_000812"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACHBJ1_000812"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM9429779.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="ACHBJ1_000813"
     CDS             31684..32451
                     /locus_tag="ACHBJ1_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429780.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="ACHBJ1_000814"
     CDS             32441..34483
                     /locus_tag="ACHBJ1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429781.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11095 a         6120 c         7248 g        10047 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat
//
LOCUS       DAYYNQ010000018        34506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000018 DAYYNQ010000000
VERSION     DAYYNQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.018"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACHBJ1_000815"
     CDS             352..1041
                     /locus_tag="ACHBJ1_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9429782.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACHBJ1_000816"
     CDS             1028..2236
                     /locus_tag="ACHBJ1_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9429783.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACHBJ1_000817"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACHBJ1_000817"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM9429784.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHBJ1_000818"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACHBJ1_000818"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM9429785.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACHBJ1_000819"
     CDS             complement(6339..7034)
                     /locus_tag="ACHBJ1_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM9429786.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHBJ1_000820"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACHBJ1_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM9429787.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACHBJ1_000821"
     CDS             complement(8233..8424)
                     /locus_tag="ACHBJ1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM9429788.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACHBJ1_000822"
     CDS             complement(8445..9335)
                     /locus_tag="ACHBJ1_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM9429789.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACHBJ1_000823"
     CDS             complement(9322..10089)
                     /locus_tag="ACHBJ1_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9429790.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHBJ1_000824"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACHBJ1_000824"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM9429791.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHBJ1_000825"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACHBJ1_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM9429792.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHBJ1_000826"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACHBJ1_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM9429793.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACHBJ1_000827"
     CDS             14660..15043
                     /locus_tag="ACHBJ1_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429794.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHBJ1_000828"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACHBJ1_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM9429795.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACHBJ1_000829"
     CDS             complement(15987..16805)
                     /locus_tag="ACHBJ1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM9429796.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHBJ1_000830"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACHBJ1_000830"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9429797.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHBJ1_000831"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACHBJ1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM9429798.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHBJ1_000832"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACHBJ1_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM9429799.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHBJ1_000833"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACHBJ1_000833"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM9429800.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHBJ1_000834"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACHBJ1_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM9429801.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACHBJ1_000835"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACHBJ1_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM9429802.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHBJ1_000836"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACHBJ1_000836"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM9429803.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHBJ1_000837"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACHBJ1_000837"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM9429804.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHBJ1_000838"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACHBJ1_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM9429805.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACHBJ1_000839"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACHBJ1_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9429806.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHBJ1_000840"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACHBJ1_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM9429807.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACHBJ1_000841"
     CDS             28113..30086
                     /locus_tag="ACHBJ1_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM9429808.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACHBJ1_000842"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACHBJ1_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM9429809.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHBJ1_000843"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACHBJ1_000843"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM9429810.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACHBJ1_000844"
     CDS             32965..34257
                     /locus_tag="ACHBJ1_000844"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM9429811.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10531 a         6709 c         6833 g        10433 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tactgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga tcaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgtaaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatca aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggtgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgg caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacat tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatggggaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 cgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaatcc ttgaaaacta
    25201 tatcgaacat caacgcgaag tcatcacacg acggacggtc tttgaaaaag aaaaagcaga
    25261 agcaagagca cacatcttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaggcaaaa gcaacaatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgtacag ccaaaggaat cccaatcatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggtcagag ggatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaaaatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata accatcaaga ctaagtgtgt aagctagcaa
    26641 gaataaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatta
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattagatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa tgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatattgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaagag gtagtgaaag agacgtttag ccttattgcc gataggctgt
    34381 ctcttgaaca ctgtttattc agaataaggg actgtgacaa aaaccggctc tttgtcaata
    34441 aggactgatg agttgtgcaa aatcaaatct gagtcagaat gaaccacatt ctggctcaga
    34501 tttttt
//
LOCUS       DAYYNQ010000019        34221 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000019 DAYYNQ010000000
VERSION     DAYYNQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.019"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(259..996)
                     /locus_tag="ACHBJ1_000845"
     CDS             complement(259..996)
                     /locus_tag="ACHBJ1_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9429812.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(998..2500)
                     /locus_tag="ACHBJ1_000846"
     CDS             complement(998..2500)
                     /locus_tag="ACHBJ1_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9429813.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHBJ1_000847"
     CDS             2759..4753
                     /gene="uvrB"
                     /locus_tag="ACHBJ1_000847"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM9429814.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHBJ1_000848"
     CDS             4763..7579
                     /gene="uvrA"
                     /locus_tag="ACHBJ1_000848"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM9429815.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            7842..9107
                     /locus_tag="ACHBJ1_000849"
     CDS             7842..9107
                     /locus_tag="ACHBJ1_000849"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM9429816.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            9111..9842
                     /locus_tag="ACHBJ1_000850"
     CDS             9111..9842
                     /locus_tag="ACHBJ1_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM9429817.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHBJ1_000851"
     CDS             9977..10861
                     /gene="rapZ"
                     /locus_tag="ACHBJ1_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM9429818.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHBJ1_000852"
     CDS             10858..11856
                     /gene="yvcK"
                     /locus_tag="ACHBJ1_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM9429819.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            11890..12825
                     /gene="whiA"
                     /locus_tag="ACHBJ1_000853"
     CDS             11890..12825
                     /gene="whiA"
                     /locus_tag="ACHBJ1_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM9429820.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            13393..14049
                     /locus_tag="ACHBJ1_000854"
     CDS             13393..14049
                     /locus_tag="ACHBJ1_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429821.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            14228..14793
                     /locus_tag="ACHBJ1_000855"
                     /pseudo
     CDS             14228..14793
                     /locus_tag="ACHBJ1_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15492..16013)
                     /locus_tag="ACHBJ1_000856"
     CDS             complement(15492..16013)
                     /locus_tag="ACHBJ1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM9429822.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHBJ1_000857"
     CDS             16211..19891
                     /gene="nifJ"
                     /locus_tag="ACHBJ1_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM9429823.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHBJ1_000858"
     CDS             20080..20967
                     /gene="cdaA"
                     /locus_tag="ACHBJ1_000858"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM9429824.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            20964..22133
                     /locus_tag="ACHBJ1_000859"
     CDS             20964..22133
                     /locus_tag="ACHBJ1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM9429825.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            22157..23512
                     /gene="glmM"
                     /locus_tag="ACHBJ1_000860"
     CDS             22157..23512
                     /gene="glmM"
                     /locus_tag="ACHBJ1_000860"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM9429826.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHBJ1_000861"
     CDS             complement(23584..25389)
                     /gene="glmS"
                     /locus_tag="ACHBJ1_000861"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM9429827.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            25799..26089
                     /locus_tag="ACHBJ1_000862"
     CDS             25799..26089
                     /locus_tag="ACHBJ1_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429828.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26102..27127
                     /gene="sppA"
                     /locus_tag="ACHBJ1_000863"
     CDS             26102..27127
                     /gene="sppA"
                     /locus_tag="ACHBJ1_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM9429829.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            27130..27789
                     /locus_tag="ACHBJ1_000864"
     CDS             27130..27789
                     /locus_tag="ACHBJ1_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM9429830.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            27946..28878
                     /locus_tag="ACHBJ1_000865"
     CDS             27946..28878
                     /locus_tag="ACHBJ1_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429831.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            29032..30321
                     /gene="tig"
                     /locus_tag="ACHBJ1_000866"
     CDS             29032..30321
                     /gene="tig"
                     /locus_tag="ACHBJ1_000866"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM9429832.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            30568..31818
                     /gene="clpX"
                     /locus_tag="ACHBJ1_000867"
     CDS             30568..31818
                     /gene="clpX"
                     /locus_tag="ACHBJ1_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM9429833.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            31901..32488
                     /gene="yihA"
                     /locus_tag="ACHBJ1_000868"
     CDS             31901..32488
                     /gene="yihA"
                     /locus_tag="ACHBJ1_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM9429834.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(32562..32717)
                     /locus_tag="ACHBJ1_000869"
     CDS             complement(32562..32717)
                     /locus_tag="ACHBJ1_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM9429835.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            32853..34181
                     /locus_tag="ACHBJ1_000870"
     CDS             32853..34181
                     /locus_tag="ACHBJ1_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429836.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        11183 a         5916 c         7405 g         9717 t
ORIGIN      
        1 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata agataatcat
       61 catagtttct gaaggtggta tcgactatgt acttgatatc gtcaatgaac cgatggtact
      121 ttcttatgcg atggaacagg tagagcgatt gcttgtttgt gcaggagaac aagcgatgcg
      181 gctggtacag ttaagtgaaa aaacaagaaa ctaaaaatgc ccggtcgtca tgaagcgacc
      241 gggcattttt ggtttttatt aaatatttaa tactttattc aagaaatctt ttgtccgttc
      301 attttgcgga ttttcaaaga tttgttgagg cgttccgtct tctaaaaagt ttccgtcgtc
      361 aatgaacatg acacgattgg ccacttcttt agcgaagccc atttcgtgcg taacgataac
      421 catcgtcata ccttgttctg ctaacttttt catgacgttc aagacatcac cgaccatttc
      481 ggggtcgagt gcagatgtag gctcatcaaa aagcatgatg tcagggttca tcgctagagc
      541 acgagcaata gccacacgct gcttttgtcc accggaaagc atttcaggat acatatcttt
      601 tttatcttct agaccaactg tctgcagcaa tttgattgct tttttctctg cttcttctct
      661 agattctttc ttcaggtcca tcggtgctaa aatgatgttt tccaaaatag aaagatgagg
      721 gaataggtta aagtgctgga ataccattcc gatatgttgt ctcaccaagt tgatatctgt
      781 gcttttatcc atcaagtggg cgccgtcaat aataatttca ccgtcagaag gttcttctaa
      841 acgattcaag caacgcaaaa atgtactttt tcctgaacct gaagggccaa tgatacagac
      901 gacatctcct tcgttgatgg acacattgat gtcgtttaat actgtgttat cgccgtattt
      961 tttgactaaa tgctcaacta agattttttc tgccatctta cttcaccttc ttttctaaaa
     1021 cttttgctaa ttttgtcaat gctgtgatca aaatcaagta catgatcgca atgatcaagt
     1081 aaacatacgt actttgtgtt gtacgtgcaa caatgatctt accagtttgc agcaattcga
     1141 ctacaccgat tgcggaaatg atcgttgtgt cttttaatga gataacgaac tgattgacga
     1201 atgatgggat catgatgcgt atcgcttgcg gcaaaatgat tttttgcatt gtccgattat
     1261 aagaaagacc taaacttctg gaagcttcca tttgtccgac aggaacggcg ttgatcccgc
     1321 ctcgaacaat ctctgcgata taagcacttg cgttaagagt caaggtgata acaccagcaa
     1381 ggaagtcagg aatcgtaaat ccaacgatac caggcaaccc aaagaaaata aagaacgcaa
     1441 gaaccatcat tggaattcct cggatgatat cgacataaat actagcaatt gtgcgaagag
     1501 cgcgtattgg tgctacactg aataatccaa aaatcacacc gattactata gccaaagcaa
     1561 aggagatcaa agttaggaga attgttctcc aaagtccttt caacaatgct tgatagttgt
     1621 tttggataag tccaagaaat gttgattcat cagcagtgga agactcactg gaagtttctg
     1681 aaccatccgc tacatattct ttaataatct tatcgtattc gcctgttctt tttaattctt
     1741 tcaatccttc attgaacata ttaagaagct caggattttg tcctttttta acggcaaatg
     1801 catattttcc gccagattct tttttgatcg gtgtttccaa ttcttgtcct tgtttgatcg
     1861 cataaccgat taccggataa tcatccatca ttgcatcgat tgcgccaact tttaacccgt
     1921 cgtacaattg atcagcagca tcaaaatatt tgatagaata gccatattca gattcatggt
     1981 cgcttaaaaa ttcagcactt tcggtaccaa cttttgctcc gactgttttt cctttcaaat
     2041 cttcataaga cttgatcgaa gtatctcctt ttttgacagc taattggatc ccgctatcaa
     2101 agtaagaatc agaaaaatca tagcttttct ttcgttcatc tgttacagtc attccggcaa
     2161 tcattccgtc tgcttgtcct gattccaaag cttgcaaagc agcagagaaa ccacggtagt
     2221 ccattcggat gttaaatcct tgcagtttgg cgatgttttc catcaatttt acatcgatgc
     2281 cttcgtactc gccctttgcg ttttgaaatt caaatggagc aaaagcagag tcactggcaa
     2341 ttacgtatac gtcttttttg ggctctattt tttgcattgt ctcgcttgtg cttgactctg
     2401 ccgttgtagt gttgtcagct tgtgcaacat gtccaaacgc aagtgtcaca agtcctgtca
     2461 ttaagaaaac aaatgagtga aataaatgct ttcttttcat aatcaacctc ctaaatttcc
     2521 aatattttat tattataggc gaaaagtgct gaatcagaca agactgtaag ctcgaaaaaa
     2581 tgaaaaaaat gcgagtttct aacattttat gttataaaac gtaacatgca taataagata
     2641 aaaaagtggc gaaagtatgt tcggtgtggt ataatttttt agtaaggaat gcctttgctt
     2701 tccttttctt tttattggtc gtaaaatgaa aaggaatgaa tgaaaaggag gacgaagaat
     2761 gattgagaga gatacctccc gacactttga gcttgtctcg aaataccaac cggctggtga
     2821 tcagcctgaa gcgattaacc aattagtgga tggcgtagtc ggcgggaaaa aagcacaaat
     2881 cttattaggt gcgactggaa cagggaaaac atatacgatt tccaatctta ttgagaaagt
     2941 gaataaacca acactaatta ttgcccataa caaaacattg gctggtcagc tgtacgggga
     3001 attcaaggaa tttttcccta acaatgctgt tgaatatttt gtttcttatt atgattatta
     3061 tcagcctgaa gcttatgtgc cttcaagcga tacgtatatc gaaaaagatt caagtgtaaa
     3121 tgatgagatc gacaaattac gacattcggc tactagttca ttattagaaa gaaatgatgt
     3181 gatcgtcatt gcatccgttt catgtatttt tggattaggt tcgccttttg agtaccaaaa
     3241 acaagtagtt tcgattcgac agggagcaga actagatcgc aatcagttga ttcgtgactt
     3301 agtcagtatc caattcgaaa gaaatgatat agattttcag cgcggtcgtt ttcgagtgcg
     3361 cggtgatgtc gtggagattt ttccggcttc aagagatgaa cgtgccttac gtgtggagtt
     3421 ttttggagat gaggtcgaac gtatccgaga agtcaatgca ctgacaggtg aagttttagg
     3481 agaaacggaa cacgtggcaa tcttcccggc gactcacttt gtgacgaatg atgaacatat
     3541 ggagcatgcc gtagcgaata tcaaagcgga attagaacag cgtttaactg tcttgcgaaa
     3601 tgaaaacaaa cttctggaag ctcaacgttt agaacagcga accaattacg atattgaaat
     3661 gatgttagaa atgggctaca cgtcgggaat tgaaaattat tctcggcata tggacggacg
     3721 taaagaaggt gaaccgcctt atacgctatt ggatttcttt cctgaagatt ttttgatcgt
     3781 ggcggacgaa tcgcacgtaa ccatgcctca agtcagagga atgtataatg gtgaccgagc
     3841 gagaaaacag atgctagtag attacggttt ccgtttgcca tctgctttag acaatcggcc
     3901 acttcggtta gaagagtttg aaaaacatgt caatcagatc atttatgtct ctgcgacgcc
     3961 aggaccatat gaacatgaac aaacagatac cgttatccaa caaatcatcc gtccgacagg
     4021 gctgcttgac ccagtgatcg aagttcgtcc aatcatggga caaatcgatg atctagtagg
     4081 cgaaataaat gaacgagtcg aaaaagacca gcgggtgttt gtaacgacat tgacgaaaaa
     4141 aatggcagaa gacctcacag attatttcaa agaattaggg atcaaagtca aatatttgca
     4201 tagtgatatc aaaacgctgg aacgaacaga aatcattcga gatcttcgat taggtgaatt
     4261 tgatgtgtta gttggaatca atttgcttcg tgaaggatta gacgtacctg aagtttccct
     4321 tgttgctatc ttggatgcgg ataaagaagg ctttttacgt agtgaacgtt ccttagtcca
     4381 aacgattgga cgagctgctc ggaatgaaga aggtaaagta atcatgtacg cagacaaagt
     4441 aacagattct atgcgtctag cgatggatga aacatcaaga cgccgtacga ttcagcagaa
     4501 gtacaatgaa gaacatggga tcgttccaaa aacgatcatt aaagaaatcc gagatttgat
     4561 ttctattacg aaagaatcgg aagatgatac aaaagaagcg gtacaagtct cgtatgaaga
     4621 aatgacgaaa gaagaaaaag atacgttatt gatgaaatta gaaaaagaaa tgaaaaatgc
     4681 ggcgaaagca ttggattttg aaacagccgc taatgtgcga gatatgattt tagaattaaa
     4741 agcttcaaag taggaggaca acatggctaa cgataaaatt acgattcacg gtgcacgtgc
     4801 ccataacttg aaaaatatag atgtaacgat tccacgagat aaattcgtgg tcgttacagg
     4861 tttgtccggt tctggaaaaa gttccctagc atttgatacg ctgtacgctg aagggcagcg
     4921 gagatacgta gagagccttt ctgcttatgc tcgtcagttt ttaggacaaa tggataagcc
     4981 ggacgtagat agcatcgacg gattgagtcc ggcgatttct attgatcaga aaacaacaag
     5041 taaaaatcct cggtcgacag taggtactgt gacggaaatc aatgactatt tacgcttgtt
     5101 gtttgcacgt gtaggacatc cgatttgtcc caacgatcat atcgagatta ccagtcaatc
     5161 agtagatcaa atggcagatc aagtaatggc tttgcctgag cgcacaaaga tccaagtgtt
     5221 ggcaccgatc gtggtaaaga aaaaaggaca acataaaaaa atattcgaac gaatccaaaa
     5281 agagggatac gtccgcgttc gagtcgatgg tgagatctat gatttaagtg aagtgcctga
     5341 gctggagaaa aacaaaaaac atgatatcgc gatcgtcatc gaccggatca ttgtgaaaga
     5401 agggattcgt tctcgtctat ttgattcttt agaggcggct ctgcgattgg cagacggcta
     5461 tgctctggtt gacgtgatcg gtgaagaaga aatgttgttt agcgaacact atgcctgtcc
     5521 ttattgcgga tttactgttg gcgaactaga accaagactg ttctcattca atgcgccttt
     5581 tggagcttgt ccagactgtg atggtttagg tgtcaaatta gaagtcgatc aagatttagt
     5641 gattccagat agaacgaaga cacttagtca aggtgctatc gttccatgga atccaatcag
     5701 ctctcagtat tatcctcaaa tgttagcgca ggcttgtgaa agtttcggta tcgatttaga
     5761 tacaccattc gaagaattac cggaagagca tcaggatatc atcttgaatg ggacagatga
     5821 tcttttccat ttccattatg aaaatgattt tggtggcgtt cgcgatgtag aaacgacttt
     5881 cgaaggggta ttgaagaata tcgagcgtcg ttatcatgat acgaacagtg atttcacgcg
     5941 tgagcaaatg cgtctttata tgacagaatt aacttgtcag acatgtaaag gttatcgatt
     6001 gaatccccaa gctctatctg ttcaaatcaa tggaacgaat atcgggcaaa caaatgagtt
     6061 gtctattcaa aaagcagtag atttcttctc aaacttaacg ttatctgaac aagagaaagt
     6121 aattgccaaa ccgattttaa aagaagtaaa tgacagactt tcattcttgg aaaatgtggg
     6181 actagactat ctaactttga gtcgcgcttc cggaacatta tctgggggag aagcgcagcg
     6241 aatcagattg gctacacaaa ttggatcgaa tctttctgga gttttgtata ttttagatga
     6301 accatcgatc ggattgcatc aacgcgacaa cgatcgactg attggctcat taaagaaaat
     6361 gcgtgatctt ggcaacaccc tcgttgtagt cgaacatgat gaagatacga tgtgggcagc
     6421 agattatctg attgacgtcg gacctggggc cggagaacaa ggcggacaaa tcgtggctgc
     6481 aggcactcct gaagaagtag ctaatgatga aaattctttg acagggaaat atctttcagg
     6541 gaaaaaatca atccctgtac caaaagaacg ccgtaaaggt aatgggaaag caatcaagat
     6601 cactggtgct tcagaaaaca atctgaaaaa tattagtgtt gaatttccat taggagaatt
     6661 tgtagcagtc acaggtgtgt ctggttctgg aaagagtaca ttggtcaata gtatcttaaa
     6721 gaaatcgtta gcgcaaaaat taaataagaa ttctgctaag ccaggtaaat tcaagacaat
     6781 ttccggctac gaaagtatcg aaaagatcat cgatatcgat caaagcccaa tcggccggac
     6841 gccgagaagt aatccagcga cttatacaag tgtatttgat gatatccgtg ggttatttgc
     6901 tcaaacgaac gaggcaaaaa tgcggggtta taagaaaggg cgttttagtt tcaacgtaaa
     6961 aggcggtcgt tgtgaagctt gtcgcgggga tggaattatt aagatcgaaa tgcacttttt
     7021 gcctgatgtc tatgttcctt gtgaagtatg tcatggcaaa cgatataact ctgaaacatt
     7081 agaagtgcat tacaaaggaa aaagcattgc tgatattttg gaaatgacag tagaagatgc
     7141 tgtagaattc ttcaagcaca ttccaaagat tcatcgcaaa ctgcaaacga ttgttgatgt
     7201 tggcttaggt tatgtgacga tggggcaacc agcaacgaca ttgtccggtg gtgaggcaca
     7261 acggatgaaa cttgccagtg aattgcacaa aatctctaat ggaaagaatt tctatatact
     7321 agatgaacca acgacaggac ttcatagcga tgacatcgcc cgcttgttgc atgtattaca
     7381 aagattagta gatgctggta acacagtttt agtgattgaa cacaatctag atgtaatcaa
     7441 aacagcagat tatatcattg atttaggacc agaaggtgga gaaggtggag gaacgatcct
     7501 tgcgactgga acaccagaag aaatcattaa cgtaaaagaa agttatacag gtcactattt
     7561 gaaaaaaata atggtataaa gaggatagat agctgagata gttaaaaaaa cataaaaaaa
     7621 tgaatttttg cttgttttat taatatcagt gttctagaat cattacatgg tagttctttc
     7681 tacactgatt ggtatagaca aaaacttcct ataagttcaa tccgttcaaa aaagaattga
     7741 gcaatcggag gagtttcctt tgattgcttt tttctttatg gtatgatatt ccttgtgtag
     7801 ttggaaaaga aaaacaagag attatgagta agtgagagat aatgatgaac agtattgaaa
     7861 atgaagaatt tatccctata ttattaggaa gcgacatgaa cgtttatggt atggcacgtt
     7921 cttttaatga agcatacggc aaaatctgcc aagcatatgc ttcggatcag ttagccccta
     7981 cccgctacag taaaattgtc aatgtagaag ttattccagg atttgataaa gaccctgtgt
     8041 ttatcgagac gatgcttcgt ttggcaaaag aaagatattc tgataagagt aaaaaatatc
     8101 ttttgattgc ttgtggtgat ggatatgcag aattgatctc ccaacataaa caagagctgt
     8161 cagaatattt tatctgtcca tatattgatt attctttatt tgaaagactg atcaataaag
     8221 taagctttta tgaagtttgt gaagaatatg atcttccgta tcctaaaaca ttgatcgtca
     8281 gagaagaaat gttagtaaat ggacatttgg aacaggaatt gccttttgaa ttcccagttg
     8341 ctttgaaacc tgcaaacagt gtggagtatc tttctgtcca atttgaagga agaaaaaaag
     8401 cttttatttt agagactaga gaagagttcg atttaatttt aggccggata tacgaagccg
     8461 gttataaaag tgaaatgatt gtacaagatt ttatcccggg tgatgatagt aatatgcgtg
     8521 tgctaaatgc gtatgtagat gaagatcatc aagttcggat gatgtgtctt ggtcatccac
     8581 tgttagaaga tcctacacca gcatcgattg gtaattacgt tgtgatcatg ccagattata
     8641 acgaaaaaat ctatcaaacc attaaggcat ttttggaaaa aatcgaatat acaggctttg
     8701 ccaatttcga tatgaagtat gatcctagag atggagaata taaattattt gaaatcaatt
     8761 tgagacaagg tcgtagcagt ttctttgtga cattgaacgg cttgaatctg gctcgtttcg
     8821 tcacagaaga tcgagtgttt aacaaaccct ttgttgaaac gacctatgga acgaatcaat
     8881 cagataaagc aagattatgg atgggtgtgc ccaaaaaaat ctttttagaa tatgctcggg
     8941 aaaatgaaga caaaaaacta gctgagcaaa tgatcaaaga aaatcgttac ggtactactg
     9001 tgttttatga aaaagaccgg tcaatcaagc gctggttgtt gatgaaatat atgttccata
     9061 actatattcc aagattcaaa aagtattttc atgtgaaaga aggctagttc atggagaatt
     9121 ttttcagtat tttaggcgga atgggcacga tggcgacaga aagttttgtt cgcttgatca
     9181 accaccgaac gaaagctaca aaagatcaag aatatttgaa ttatgtctta ttcaatcatg
     9241 caacagttcc tgatcgtaca gcttatattt tagatcgatc agaagaaaat ccaatgccgt
     9301 ttctgctgga tgatattgag aaacaaaatt tattgcagcc gaacttcatt gtgttgactt
     9361 gtaacaccgc acactacttt tttgaagaat tacaagcggc aacggatatt cctatccttc
     9421 atatgccaag agaagcagca aatgaacttg tccgtcagca tacgacaggg agagtagcga
     9481 ttctaggaac agaaggaagt atgaaagctg gaatctatga acgcgaagtc aaaaatcttg
     9541 gattcgaaac agtgattcct gatactgttc ttcaagaaaa aatcaattat ctgatctatc
     9601 atgagatcaa ggaatcggat tatttgaatc aagagctata ttacgaaatc ttagaagaag
     9661 cggtagagcg cctaaactgt gaaaaagtta tcttaggttg cacagaattg tcattgatgc
     9721 atgaatttgc tgaagataac cattatccag tgatcgatgc ccaatctatt ttagcagatc
     9781 ggacgattga acgagcgcta gctgaaagaa gcaaggcatt ggatacagca tcggaaaagt
     9841 aaaaaaattg gatttggggc aaatatccta aaagatattt ggtccaaatc tttttgtaaa
     9901 agaaaaagaa aactggtaat gatacgcccg gaatgatata attttactat gcacaatgtg
     9961 agaggagttc gtaattatga cagataattt gcaattagtc atcataacag gtatgagcgg
    10021 tgcagggaaa acagtagcta tccaaagttt tgaagatatg ggctattttt gtatcgataa
    10081 tatgccgcct cgattgattc caaaattctg ggaattaatc aaagaatcag gcaaagtcac
    10141 aaaaatggct ttaggagtgg atttacgttc tcgttccttc tttgaagaaa tccaaaacat
    10201 gctgatcgaa atcgaaaata cgaacttcat cgacacttct atcttatttt tagatgcttc
    10261 agacgaagaa ctagtggctc gctacaaaga aacaagacga gcacatccaa tggcgatgga
    10321 cggtttgatc actgaaggga tacgtaaaga acgggcgata ctagacgact tgaaagccca
    10381 agcttccgtg atcattgata cgacgacatt gacaccacga caattacgtg aaaaaatcaa
    10441 tgagtcattc aaatcaaagg gagaccaagg atttcggatc gaatttgtat catttggttt
    10501 taaatacggg ttgccgatcg attcggatat tgtgatggat gtgcgttttt tgcccaaccc
    10561 tcattatatc ccggaactaa gaccattgac cggaaaagat cagccagtct atgattatgt
    10621 gatgtccttt gatgaaacgc aatcattcta ccataaattt ttggatcttc tggaaactgt
    10681 attgcctgga tacattaaag aaggaaaaag cagcttgaca gttgcaatcg gctgtacagg
    10741 cggtcaacac cgttctgtgg cattgactga acgtttggca aatgctttag ggaaaaacta
    10801 taaagtcaat gtgacacatc gggacaagga taaaaggaaa gagacggtga accggtcgtg
    10861 aaaatgaaaa cctaccggat aagaaaacca aagatcgtag tggtcggcgg tggcactggt
    10921 ctgccagtta tcctgaaaag tttgcgaaat caaggagcag atatcacagc agtagtgact
    10981 gttgcagatg atgggggaag cagcggtgca atcagagaat cgatcgctat ggctcctcct
    11041 ggtgacttga gaaatgtctt agtggcgtta tcggatatgc cacaattcta tgaagatatt
    11101 ttccaatacc gtttcaaaaa ggaagatcag tttttagcca atcatacgat cggtaatctg
    11161 atcattgccg cagtttccga gatgcgggga agcacttatg aggcgattca attattagca
    11221 aaaatgatgc atgtagatgg acatgtttat ccttcttcag aaactccttt gaccttgcat
    11281 gctgtcttta aagacggaac gactgcagta ggagaatcta aaatcgcgat cgaccgtaaa
    11341 accattgacc gtgtgtatgt ctgcaacaca aatgataaaa gtgaagccaa agcggctcga
    11401 aaagtcgttt cagcaattat ggacgccgac atggtcgttt tgggacccgg aagtttattc
    11461 accagtatcc ttccaaattt agtgatacca gaaatcggcg aagcaatgct caagacaact
    11521 gctgaaaccg tctatatctg taatatcatg acccaaaaag gggaaacgga acatttcaca
    11581 gatgccgatc atatacgtgt tttacacaag catttgaatc agccttttat cgatacagta
    11641 ttggtcaata cggaaaaagt gcctgaagat tatatggacc cagagatcta tgatgagtat
    11701 ctagtacaag tcgcacacga cttcaacgga ttgagaaatg aaggatgtcg agtgatttcc
    11761 accgactttt tggaattgaa agatggtggt gtcttccacg atggagacaa agtagtagaa
    11821 gaattgttcc gtttagtttt cggttcaaaa tattaagcaa tttattgttg gagaaaggag
    11881 gagaatagga tgtcatttgc tgcagatgtg aagaaagaac taactggttt ggaagttcat
    11941 cgagaacatg cgaaagcgga attagctgct ttgctgcgaa tgaacggctc tttaggtatt
    12001 gtgaaccagc agtttatttt gaacgtccaa acagaaaatg ctgcaattgc cagacgtatc
    12061 tattctcttt tgaaagatca ttatcaggta cgaagtgagc tactcgttcg taaaaaaatg
    12121 aaattaaaaa agaacaatgt ctatatcgtc cggttgaagc aaggcacaaa agatgtgttg
    12181 atggatcttg atatcatgga tggtatgatg ttcaatggac acgtttcgga cgagattatg
    12241 ggaaattcgc aaaaaatgcg ttcttactta cgtggtgctt ttatggcctc aggttcagtc
    12301 aataatccgg aaacgagccg ttatcatttg gaaatatttt ctatttatga acagcataac
    12361 caagacattt gcgatatgtt aaattattac ggtctacatg ctcgaacgct tgaacggcgc
    12421 aatggctata tttcttattt aaaaggagca gaaaaaattg ctgatttctt aacattgatt
    12481 ggtgcaacca actcaatgct ccgatttgaa gatgttcgaa tcgtacggga tatgagaaat
    12541 tcggtcaatc gtctagtcaa ttgtgagaca gcaaatctga acaagacgat tgatgcggct
    12601 tctaaacaaa tcgaaaacat cgaattgatc gaagcaaagg tcggtctaca tgcccttcct
    12661 gaaaaacttc aggaaatcgc agaacttcgg cttcaacatc cagaggtcag cttgaaagaa
    12721 ttaggggaaa tgattccctc aggtgcgata tcaaaatcgg gaattaatca ccgaattcgc
    12781 aaaataaatg aatttgcgga aaaactgcgt gaagaagcag tttaatccaa agaaaaaact
    12841 gatacatgaa gcaggagatg cgaatgtatc agtttttttt aaaagttata cttgcttttc
    12901 ttccgtcaaa ctataattcc atctttactg gagtaacata atttggaaag gcttctattg
    12961 tttttaatac aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttgtac
    13021 ataaattttt gcttaatata gaagtcctga tatagataag atcaagtatt tatctataac
    13081 tggcctgatt aattaaaaaa ctggaaggaa ctgctcgatc atccttgaga actgctcttc
    13141 ttacttctgg tcaagattct cgtggttggt ggataaggat ggttgataag ttcattggat
    13201 tagctagtcg aaaacaacca ggtgtaatta atgtagaagt tttaacaagg ggatggagtg
    13261 atttattcag tgataatcat aagttcataa atagtaattg agatgtatgg ttggaatacg
    13321 ttatacctga acttgaacta ccaagatttg gtttagaatt tgagaatcga tgtttataaa
    13381 gggagggcaa aaatgcgttt ttttacaact tttactatca ttatgattgc agtacttttt
    13441 atttttctag atatagcaaa gcgaaataca gcatttttat tatatcgggt tttacttcga
    13501 gcaggtctca taacatttat atcgatcgtt ggattttttc tattcacagt tattgtattt
    13561 atttggagaa ctccagcacc accattgcca gagattacat atggagaatt tccttttaga
    13621 cttgagtatg aattgaatga agaattacat gtgatagaag acacgttaat agttgaattt
    13681 gatggatttg gaatgaacga aggcataggg agatatcgta gatggacaag tcgtttagcc
    13741 agcggagaag atttagtgtt gctgttagaa gttagtgata ataagcaaat attttatttt
    13801 ccaggacctg ctaattatta catgggagat aggttgaatg gttataatca tacttttcct
    13861 agtgcttctt tcattgaaag ggaaagaggc attactagaa gggatatttt acatgacaaa
    13921 gaattattag aacaatttgg tccgcttgat caaaatacaa taaatgaaga agaattgtta
    13981 aatcaatata atataaggct agtgaattgg gaaatatctg aaccgattgt taacaatttt
    14041 ggtgattaga aaacaattac gataaataga aacaagccaa atcatcagca agtaaaagct
    14101 gatgatttgg cctttcttat acgagtagta ggaattcata gcgaatatta ttacgatcag
    14161 tagatatttt gatattaaag gagtattacg aactagtatg tgagaatgta gaggtgattt
    14221 gcgaataatg agaaatttat caaatagaaa caaaatatta atcattattg ttgttatagc
    14281 tgtgtttcat ctgggaacga atgcggtttt aagtagaata atattgggtc ctaagccacc
    14341 aagacctgcg attacccgag gggagtttga ttttagatta gaatatgaag tagatgggga
    14401 aaggattgtg atagaagata caattgttgc attatttgat ggattttctg cagatgcagg
    14461 tagcatggca tggtatcgca cgtggcgttt gcatttggcg agtgacaaga gggcacaaca
    14521 attattgtta catgagttag atgatggtcg aaaaatatat tatcattttg gaggagctgg
    14581 attttatatg gggaagttag tagcagtgta actgtaacaa ggaaagattt gccaggtctt
    14641 ggcattcgaa actccatatt ttatatcgag cgtgggagag atacatctat tgggaacagg
    14701 aaagggttaa ctcttgaaga gttatataat gactttggaa tccgtcttgt tagttgggaa
    14761 cacaatccac caatagaaaa tcgctttgaa taatcgaaat ctattaatcc atttcatcag
    14821 ccagtgaatg ttgtaatgga acaatttggt ttagtgttca aaaccattga aatactaggc
    14881 tgtgatcagc aaaaatgtat ctgaataaaa aagagagcaa ctattaaatg agaggagtta
    14941 ggagttgtta gctttatacg aatcaaagtg gcaaatacag atgcgtatgc atttgtgttt
    15001 gccatcttgc gtttggtaga cgagataggt ataagctaaa gaaacaactt gtttaaaata
    15061 aacgacctag ctcaacaaac gaccatctaa tcaactaaca gcatacataa aagcaactat
    15121 ttttgaagta gaattgaaat cgggataact taaaaagtga ttgggatact agtgtgaatc
    15181 accgtacata aatattactc gttaagggaa tttgagaaat tttgtatgaa aatttaagaa
    15241 ttagttagta tgcgcaagtc aaaaatatga ataaattatt tggtggagat aatttgctca
    15301 gcttgtcata agaaatgaga taatctggca gatgcaagct tttggacacc atttattcgg
    15361 aataaggagg ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct
    15421 tccgccgttt ataggtgttt agagcgtgag acaaaagtgt tctttacttt tgtcctacgc
    15481 tccttatttt tttattttgt ttggatcaat tcttttaatt cctctattga aatagattca
    15541 tcaaagattt ctttacccaa gatgacagtc ggaacaaatc gaatatgtgc ttgctcagct
    15601 tcgttgatga cagcttggct cagctgttcg tctttttgtt ctgtcagctt tagtttatct
    15661 actgcaaaat ccgctacttc ttggagagaa agatgtttcc actcgtcttg agtatcgaat
    15721 atttgtttga tctctttgat cgctttttga ccatcgctga ttgtgagata ccggtgcatc
    15781 acgtttcctc gctgcaagct ttctttttct ttatcgaaca atttgatcac tcgctgcaat
    15841 tgcccgcttt ggactgcttc ttctaatagc tcataagatt cttcaaacca ttgtcggcaa
    15901 tacggacaag ataggttgat gaattcgacc atttttttag gagcattcga tgaaccataa
    15961 aggattcctt tttgtgtatt tgttttcgtt gcatcaatca ctgatatatc catgttaaaa
    16021 cacctctttc gcttatagtt tatacatgtc tgacaaaaaa aggcaacgat ttagactgtt
    16081 tgtctaaaaa ttagatgacc aattcaaata gacatatgtg aaatattttt ataaataaaa
    16141 gaaaaaatgg aatgttttac gttataatgg aacagataat aagagagaat atgaacaagg
    16201 atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat acggcagccg catatatttc
    16261 ttatgcattt accgagattg ctgcaatata tccaataaca cccagttcga ccatggctga
    16321 attagtagat caatggtcag cagcaggaaa agaaaatgta ttcggtcaac cagtaaaagt
    16381 agtagaaatg caatccgaag caggagcagc tggtgtggtc catggatcat tgaaaactgg
    16441 tgcactgaca actacatata ccgcttctca agggctgctt ttgatgatac cgaatatgta
    16501 caagattgct ggggaactac tgccaagtgt tttccatgtt gctagtcggg cagtgacgac
    16561 aaatgcgttg aatattttcg gagatcacgg agacgtgatg gctgcaagac aaacaggatt
    16621 tgctatgtta gctgaaagca gtgtacaaga agtgatggat ttagcacctg ttgcccatct
    16681 tgcttctatt gaaggagaag tacctttttt gaatttcttt gatggattcc gtacgagtca
    16741 cgagattcaa aaaatcgaag ttttggaata tgaagaatta gctcctctga tcaatcagga
    16801 gaaattagct tcatttcgta aacgagggat gaatcccaat catccgactg ttagtggtac
    16861 gaaccagaat ccagacatcc attttcaaca acgggaaacg atcaacccct attatgataa
    16921 attacctgaa atcgttcaaa aatatatgaa agagatcaac cgattacgag gaacttctta
    16981 tgacttggtg acttattacg gagcagacga tgcggaagaa gtcattgttt ctatgggatc
    17041 agttgcacag acgattgaac aaacgattga ttacttgaat gcaaacggac ggaaaacagg
    17101 ttttttgaat atccatcttt atcgtccttt tccagtggaa acattcgtgc aaaagttgcc
    17161 tcatacagta aaaacaattg ctgttcttga tcgaagtaaa gaaccagggg caggtggaga
    17221 acctctctta ttagacgttc aaagtgcgtt atatgattct gaacttcgcc cggctgttat
    17281 tggtggtcgc tacggattgg ggtctaaaga tgtgactccc gaccaaatct cagcagtttt
    17341 tgatgaattg aaaaaagacc caagcatacg caaaaaacgg tttaccatag ggatcgtgga
    17401 tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg gatttgacag aaccgcaaac
    17461 attccaggcc aagttttggg gatttggatc tgacggaaca gttggagcaa ataaatcagc
    17521 tatcaagatt attggagatc acaccgataa atatgcgcaa ggctattttt attatgattc
    17581 gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt ggagatacgc cgatacgttc
    17641 agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac acaccagctt acctgcatag
    17701 ctatgacttg gtcaaaggat tgaagccagg tggcattttt ttattgaata ctctttggag
    17761 cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa cgttacttgg ctgaaaataa
    17821 cattagattc tatacgatca atgccatgag attggcccaa gaagtcggtt tgggacgccg
    17881 gatcaatacg gcaatggaaa cggctttttt taaattagct gacatcattc cttttgatga
    17941 ggtgcttccg ctgctaaaag aagaagccct gaaaagctat ggacataaat caatggctat
    18001 cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa ctgcttcatc aagtcgcgat
    18061 cccagaccat tggagaacgt tggagatgcc tgaaaaaatc aataaaagga acactggtta
    18121 tatacaagaa atacttgaac cagtgaatgc acaggaagga aatcaactct ctgttggtac
    18181 acttgtagca aatggaatga caactggcga aatgccttta ggaacggctt ctatggaaaa
    18241 gcggggcgtc gctttagaag tacctgagtg gattagtgat cgctgtacga tgtgtaatga
    18301 atgtgccttt gtttgtcctc atgctgccat ccgtccattt ttagcagatg aagaggaaat
    18361 ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga ggcgcagacg gcgtaaaata
    18421 ccgtatccaa gtttcagtgg aagactgtac aggttgtggt ctgtgtgtag aggcttgtcc
    18481 cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa cagaaagaac aagctgtgaa
    18541 ttgggctttt gcaatgacat taagacaaaa agcaaatcca gtcaaaggaa agaatactgt
    18601 cttgtctaca caattcaatc aaccgcttct tgaattctcg ggcgcttgct caggctgtgg
    18661 cgaaacacct tatgtaaaat tattgacaca aatgttcggt gatcgaatga tgatcgcgaa
    18721 tgcgacaggc tgttcttcta tttggggcgg tgcttctcct gcttctcctt atacgaccaa
    18781 tgaatgtggt caagggcctg catggtcaaa ttctttattg gaagataatg ctgaatttgg
    18841 ttatggtatg cttctagcgg cacagactcg tcgcgaggct ttggcaagtt caatcaaaga
    18901 agtattgcca aaagtatcag aagaactacg agaattgatg gaagactggt tggtacacat
    18961 gtttgagagc gaaggaacgc agcaaagagc agcaaaactg caagctgcga tcgaaagcga
    19021 acgatcaatt gtccctgaac tggaggatat atacaagaga aaggatctat tcgtaaaaaa
    19081 cagccaatgg atgattggtg gagacggatg ggcttacgac attggttttg gcgggatcga
    19141 tcatgtactt gccagcggag cagacgtgaa tattcttgtt ttggataacg aagtctattc
    19201 caatactggc ggtcaaacct caaaagcaac accagcttcg gcaatcgcta aattttcggc
    19261 tagtggaaag tatgcttcaa agaaagatct aggaatgatg gcgatgacat atggaaatgt
    19321 ttatgtggcg caaattgctt caggtgccaa tcaaatgcag acgataaagg cttttgaaga
    19381 agcagaaaag tatcctggac cttctttaat cattgcctat acgccttgta tcacacatgg
    19441 tttgcttggt ggaatgaaag aatcgcttaa agaagcaaaa gaagctgttt cttctggcta
    19501 ttggtcatta tatcgatata accctgcaat ggtcgaaaaa aacaagaacc caatgacttt
    19561 ggactacaaa aaacctgatt tctctgctat gcctgatttc atgaggaaac aagttcgctt
    19621 ttcctcgcta gagaacgcaa atccaaatct tgcaaaagct ttatttgaaa agactatcca
    19681 agacgctaaa acacgttttt acaactatgc tgcactgacg ggagaattag aaaaaatcaa
    19741 gaaacgactt gaaccagagg aatctatcga tgtacccgtt aagactagaa aagaaaaaac
    19801 gacggacctt gaagcagaag aaagaagagc tgccagagca gaacgacgta aaaaaatgga
    19861 acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat aattctctga
    19921 agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt tcctgaaaat
    19981 tatgcgtttt tctcaagaaa aatacgagca gatcagcctc taaaattaaa acatatgata
    20041 tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca aatcggggaa
    20101 ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc tctgaatttt
    20161 ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact gatccaactg
    20221 ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat tgttatccga
    20281 atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca agtcattacg
    20341 tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg actggaacat
    20401 cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga tgagaagatg
    20461 atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg tgcgctaatt
    20521 actatcgaac ggcatactgg attagatgag tatattgaga caggaattgc attagatgca
    20581 gatatcactg gagagctgct gatcaatatc tttattccta atacaccgct gcatgacggt
    20641 gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc tctatcagaa
    20701 agtatgttga ttccaaaaga attcgggact cgacaccggg cagctgtcgg tataagcgaa
    20761 gtcagcgacg cgattaccat cgttgtctct gaagaaactg gggatgtcag cattacatta
    20821 gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatttt gcgtagagaa
    20881 ctgattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt cctagacggt
    20941 atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc attctttaca
    21001 gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc cagtccgttc
    21061 caaatacgct ttctggaaat gaagcgtata atcaaacagt tactggggtg ccaatcaaga
    21121 tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg actgtcaaac
    21181 tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca cggatgttcc
    21241 gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaggttccg ctaaaaattc
    21301 aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca gtaacggttg
    21361 agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg ttcacaccat
    21421 ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc actacaggtg
    21481 acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg atggtcacag
    21541 atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg gaagtattaa
    21601 gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag ccaagcaagt
    21661 cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa agctatgatt
    21721 tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt gcatcgatcg
    21781 gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca actcgtaaaa
    21841 ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt gtggagatta
    21901 cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg acacaagaaa
    21961 atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca tcgacaacaa
    22021 ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca gaagaaacat
    22081 cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat taagttaaag
    22141 gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg gtgaagccaa
    22201 caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt atgtcctaag
    22261 ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata caagaatctc
    22321 aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta tcgaagtgtt
    22381 ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc aaaaagcaag
    22441 cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa tcaaattttt
    22501 cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag cattattaga
    22561 tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg tagaagaatt
    22621 tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg gtgacctttc
    22681 cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg ttaatcgttt
    22741 gtttgccgat cttgaaacag atttttatac catgggcaca aatccagacg ggttaaatat
    22801 caatgatggc gtaggctcta cccatccaga acgtttagct gagatggtcg ttgaaaaagg
    22861 agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg tggatgaact
    22921 tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctagatact tggcagaaaa
    22981 aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag gattccacaa
    23041 agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc gttacgttgt
    23101 agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc acatgatttt
    23161 cttagatttt aacacaactg gagatgggat gctttcagga attcagttac tgaatatcat
    23221 gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct atccgcaaaa
    23281 attagtgaat atccgagtaa gtgataaaaa cggtgcgatg gatgttccgg caatcaaaaa
    23341 agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag ttcgtccttc
    23401 cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga aagttaatta
    23461 ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt aagagatatt
    23521 ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt ccttgttctt
    23581 ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt cgaatccccg
    23641 taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta gagtagttag
    23701 ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc cctctgagac
    23761 aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc gagtatgcgc
    23821 accagtcact tcttcactga tgattccaat cactggtgtt ccttcttcga tcaaagcaat
    23881 cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac tgatttcttt
    23941 caatttcaaa gcagcttcaa gagaaacgtt gtagtcagcg ccacgtccaa tgtaaaaggc
    24001 atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt tttgggcgat
    24061 gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga atacaagaga
    24121 agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta cagctatttg
    24181 tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca atagcagtgt
    24241 aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa gtgaagggta
    24301 gccgagacgg ttgattttta ccagaacttg gcggctatct gctgtttcgc cactttgact
    24361 caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa attcactggc
    24421 taaatgaacc tcaacaggga tctgtgtcaa tttttccaga gtctgtttac ctgctaaccc
    24481 cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag acaatgtatc
    24541 gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt gagcgatacg
    24601 acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat aggtaccttt
    24661 ctcgatatct gaagaatcta cttgtgcttc gaatgaagaa cgttctacga tgtcacctgc
    24721 aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat cttcaatctc
    24781 aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca cattgaatcc
    24841 atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa catcaggtgc
    24901 tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag cttttaaaaa
    24961 cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga taatctcagt
    25021 atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt cttcaaagtt
    25081 ttcgattacg ccattgtgaa cgagcacgaa gcgtccactt tgggaagtat gtggatgggc
    25141 gttttcttca gaaggttgcc cgtgagttgc ccagcgtgta tgaccaatac ctatatttcc
    25201 tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg ctttgaccag
    25261 ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac ggtattctaa
    25321 tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca ttccaacaat
    25381 tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct tccagctgca
    25441 ggaaaatcct tttgttttat ctttgcagat attttttgtg gattttctgt agagtgcagt
    25501 ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg tttacacaga
    25561 cctggcgcta tcctaaaagg tataactatc ctcctctttt gttttttatc tggaaacatt
    25621 taaaatcata accttttgcg aatgttcttg tcaacaaaaa aagaataaaa aataaattgg
    25681 tatatatcaa atgctttgtc aaatgctctt tttatgtcaa aaaataatgg gtttttatct
    25741 atcatctttt tcaggagttc ggtacaatag aaagtaatcg gatagaggag gagtaaaaat
    25801 ggattcaaaa attgagatca tgacactggg aatgttaaaa aaacaattgt ccaaattcga
    25861 agcatcagct ggtgtatcag atgatacgaa aatattttta gatactggct gggatagcat
    25921 ccaagaaatc gcacctgatg ccttggaagt tgctgaagct cgtgagttta cggtagaaga
    25981 tgaattgaca aaagagtcgt tttccgggta tgcgcgggaa gaaaaagcag agcggttcga
    26041 cacttctgag cagtcagaaa ctgtgatcgt cataaaaaac ttgtattaga aaggagaaag
    26101 aatgaataag agacgttggg cagcagttct gattgctgca ggattatttg tggtttcgtt
    26161 agtttcagct agtttgacta agcctcagga aaacgatcaa attgagggat tgaacagttg
    26221 gttatatggg gatgaagaac tagcttcaaa tgtgttggaa gagggagaca gtacgcaaaa
    26281 aatcgtaaaa ttatcagtag atggaacgat tgctgcggga gggtcttcag gtttatttgc
    26341 ttctgaaggt tacaatcata aaaattttat ggaacaactg aaggcgattg aagaagatcc
    26401 aacagtcaaa ggtatctttt tagaagtaaa ttctcctggt ggcggtgtct acgaaagtgc
    26461 ggaaatcgct aagaaattgg atacgattcg taaagaacat gatatcccaa tgtatgtcag
    26521 catgaaaaac atggcagcta gcggtggcta ctatatttct gctcaagcag ataaaatctt
    26581 tgcaacagaa gaaacagtga ccggctcaat tggtgtgatc atgtcaggat tgaattattc
    26641 aggacttcta gaaaaactgg gtgtggaaga tacaacagtt aaaagcggag cgttgaaaga
    26701 tatgggttca ggaacgcgac cagagacaaa agaagaagaa gcggtgcttc aagcatatat
    26761 cgacagcgca tatagtcgtt ttgtagcaat cgtcagcgag ggacggaata aatctgaaga
    26821 agaagtcaaa aaaattgctg atggaaggat ttatgatggt gtccaagcaa aagaagtagg
    26881 tttagtcgat gaattgggat ttcctgaaga tgctttaaaa gctatgcgaa aggaacaaca
    26941 gcttgaagat gcggaactag tagaatattc ttccacgcca tctgggtttg ctagtacatg
    27001 gttaggaagt aaactagcag aattccaggg attgaagcct tctgagacta gtcagatctt
    27061 atccgttctt gagaatctag ggacagcgga atcaccaaaa gcaatgtatt attacggagg
    27121 tgagtaagca tgtcagagct ttcaaatgaa aataaggaac aagaaaaaga taagcaacag
    27181 cagcctgctg taggacctga ctccaacttt tcccaaaagg tactgagctc ttttgaaaga
    27241 gagcctttga cgcccgaaga aatcaaaaag aaacaacgta aatggaaaaa gtatacggac
    27301 acaagaaatg aaccgccaaa agcaaatgat tttcctaagt atttctatgc aggttttttt
    27361 atccgaatgt ttgctttctt ggtcgatctt ttgtgtatcg gagctattac gcgtttaaca
    27421 cttggtattg ccaaaaatct aggttggctt aatttttcag attcttatct gagtatctac
    27481 ggattcacag ccttacttat ttatctagcg tattttattt tattgacgaa attcaatcat
    27541 ggtcaaacga ttgggaaaat gatctttggt attcgagtca tcagctttga tgaagaagaa
    27601 cttagctggt caaccgtttt agttagagaa ggtgcttgcc ggttcattct aaaatttcct
    27661 ttgctgatgt tagggtatct tccagcaatc tttaatcaga aaaagcaaca tctaggcgat
    27721 tttttcacta atacaagtgt tgttacgatc aatctgatca aagcattcaa ccaggaagtc
    27781 aacagctgat gagcaataaa aagcagtaac agttgaaagc accttctaaa agtcagatct
    27841 aaaatctagc ttttagaggg tgcttcagtt atttttaatt gtcatgcttt ttttgttttg
    27901 ctactattga acatgagctg aacatgagca agaggaggac actgcgtgaa gccgattgat
    27961 tttccagaaa aatatgagaa tttaatgcga gtagcacaac aagcactagc aaaccagcaa
    28021 tatcagcagg caaaagaatt atttcaacga gcttacgagt tgaaagaaag ctttgaggct
    28081 aatagcctgc ttgttttttg tctttacgag ttggacgaga aaaaagaagc attgaagcaa
    28141 gcactcttgc atgaaaaaca gtatcttcaa aatgaggagt ttgctgaatt ttattttgac
    28201 ttgttaatca gtacacaaga ttttttatat gctagaaaat tgatagcaag tacagatttt
    28261 tatgaagctt tcgagcagcg ggtcattgaa aaaatccaat ttgcagaaga attatccgga
    28321 caaatggaga ggcagaagat caaggcactc cacaataaat cggaggaact tccttcgttg
    28381 gaacctgcac gtcagcttgc gtctattgat gagatcgaac agcttcctta tcatgagttt
    28441 atccaaacag cgagtaaact tattgtatcg ccagaggtcc atattttagc aagggcaaaa
    28501 ttactggaga cacttaggca attgaacgaa tgccatcctg ttttctattt gacgattgaa
    28561 gaaaagttag tcaaagtcat accaaaagat ctgccaaagc cgcagcagca ggcaagttac
    28621 cagcaattat gtgtattttc agatcgttac ggtaatgaag atgcgctgct tagttccgtg
    28681 ttaaatgaag aattcacgtt gcaatctgca attgtttatc ctgtctatga tacatacatc
    28741 gaagacccga agcgctggtt tcaactgact gtagaggcgt gtacaggaaa aacgatgtat
    28801 gaaggtacag aagatgaaaa acaagatttt ttaaaaaaaa gagaaaaaat tttgcagcaa
    28861 atgatcttct tccattagat tttatcagtg aaaaagataa aaatgttatg aaaaacaagg
    28921 attctatcca ttcaatatgt ttacaatttg aaagggttag tgtactattt aaaggtatgc
    28981 atgaaacgtt tttgatgtat aaaatgaatt tctaaactcg gagggaatta aatgactgca
    29041 acatttgaaa aaaaaggcac gaatgatggt gttttaaaat ttacgattgc gcaagacgaa
    29101 atccaaaaag gattaacaac tgcgtttaat aaagtgaaaa aaagcttgaa cgtacctgga
    29161 ttccgtaaag ggaaagtttc tcgtcaagta ttcaaccgta tgtacggtga agaagcttta
    29221 tatgaagatg cattgaatgc tgtattacca gaagcttatg aagctgctgt caaagaagct
    29281 ggcatcgatc ctgtttctca accaaaaatc gatgttgaaa gcatggaaaa aggtcaagac
    29341 tgggtaatca ctgctgaagt aacagtgaaa ccagaagtaa aattaggaca atataaagat
    29401 ctagttgttg aaaaacaaga ccgtgaagta acagatgctg acgtggatgc acgtatcaaa
    29461 cgtgaacaag aagcacaagc tgaattagtt atcaaagaag atgctgcagc tgaaaatggc
    29521 gatacagtag taatcgactt cgaaggcttt gtagatggcg aagcgtttga tggcggaaaa
    29581 ggcgaaaatt actcacttga attaggttca ggctcattca ttccaggttt tgaagatcaa
    29641 ttagttggtc ataaatcagg tgaaagtcta gatgtaaatg taactttccc agaagattat
    29701 caagcagaag atcttgctgg aaaagaagca gtcttcaaag tgacaatcca tgaagtaaaa
    29761 gcaaaagaag tgcctgaatt agatgacgaa ttcgcaaaag atgttgatga cgaagtatca
    29821 acattagacg aattgaaaga aaaatatcgc aaagaattag tagaaacaaa agaaaaagca
    29881 gcggacgatg caaaagatga agcagcgatc cgtatggctg ttgaaaacgc agaaatcgtt
    29941 gaattgccac acgtgatggt tcatgatgaa gtacatcgtt caatggacga attcttgaac
    30001 aacatgcaac gtcaaggtat ctctcctgaa atgtactatc aattaactgg ttctacagaa
    30061 gaagacttgc ataaacaatt cgaaggtgaa gcagaaatgc gcacaagaac aaatcttgtg
    30121 atcgaagcaa ttgcagctgc tgaaaatctt caagcaacag aagaagaaat caatgcagaa
    30181 atccaagaat tatcagaaac atacaacatg ccaatcgaac aaatcaaacg cgtgttgaca
    30241 gaagatatgc tggaacatga tgtgacaatg aaaaaagcaa tcgaattgat cacaggtaca
    30301 gcgacagaag cgaaagaata gaaatcaagt aataaatcat ttctgaagaa gctacttcta
    30361 gaaaattgtt tattcgaaat aagggactgt gacacgattt tgtcacagtt ccttattttt
    30421 tgttaagagt aaaaaatgca aaggtatagg tcagttgatc ctttgttaaa cactttttct
    30481 ttttactaaa ctatgttatg atagtaaaag actgtaaagt ctttgatagt ttcttgcata
    30541 ctttttgcaa gttaaggggt gaagaccatg tatgacaatc caagtagcaa tgagaccgta
    30601 cgctgttctt tttgcgggaa aacccaagaa gaagtgaaaa agatcgtagc tggtccaggg
    30661 gtctacatct gtaatgaatg tatcgaccta tgtaaagaaa tcattgatga agaattttat
    30721 gaagaagctg tacgcgaatt cactgaagtc cctaaaccac tagaaattct tgaagtattg
    30781 aataactacg tgattggaca agatcgcgca aaacgctcgt tagctgttgc cgtatacaat
    30841 cactataaac gtgtcaatca gcaagcacaa gaaaacacag atgatgttga actgcaaaaa
    30901 agcaatatct gcttgatcgg acctacaggg tctggtaaaa catttttagc acaaacatta
    30961 gcaaaaacat tgaatgtacc ttttgcaatc gcagatgcga caagtttgac agaagctggt
    31021 tatgtggggg aagatgtaga aaacattttg cttaaattgc ttcaatctgc tgattacaat
    31081 gtggaacgtg cagaaaaagg gatcatctat attgatgaga tcgataaaat tgcgcgtaaa
    31141 agcgaaaatg tatctattac tcgtgacgtt tccggcgaag gtgtccaaca agccttgctg
    31201 aaaattctag aaggaactgt tgcaagcgtg ccacctcagg gaggacgtaa acatccacat
    31261 caagaattta ttcagattga tacgacgaac atattgttca tcgtaggtgg tgcatttgat
    31321 ggcatcgaaa cgattgtgaa gaatcgtatg ggcgaaaaga cgattggatt tggtacacaa
    31381 aaccaaaaac tttctgaaga cgaaagtgtc atgcagcata tcattcctga agatttacta
    31441 aaattcggat tgatccctga gtttatcgga cgattacctg ttatggcagc tttagagaaa
    31501 ttgacaacag atgatctggt acggatcttg actgagccga aaaatgcgct agtcaaacaa
    31561 tatcaaaaac ttctttctct agataatacc gaacttgaat tcgaacctga agcgttgaga
    31621 gccattgcga aaaaagcaat cgaacgcaat accggtgctc gaggacttcg ttccatcatt
    31681 gaagaaatca tgatggatgt gatgtttgat atcccttcaa atgaaaacat cgaaaaagtg
    31741 attatcacaa aagaagcagc tgaattgtct ggtgagccaa cagtaatcta taaagattca
    31801 gagaaaaaag cgggttgatg aaaagaggct gccgtaaaag tcattttggc ttttgcgaca
    31861 gccgcttgct ttattcatca ccttgttaag gagagagacc atgaaagtcc atcaagctga
    31921 aattatcata agtgcagtgg caccaaaaca atatccagat acacaattgc cagaaatagc
    31981 tctggcagga cgatcaaatg tagggaaatc ttcttttatc aacacattga ttgatcgaaa
    32041 gaatttagcc cgtacttccg gtaagccagg aaagacccag acattgaatt tttatttgat
    32101 cgagaacgca ttacattttg tcgatgttcc gggatatggg tatgcaaaag tatccaaaac
    32161 tgagcgagca aaatggggaa agatgatcga gacatattta acgactagag accagctacg
    32221 tgcggtcgtt tctttagtag atttgcgtca taagccttct caagaagata tccagatgta
    32281 tcaatttttg aagtattatg atatccctgt aattgttgtt gccactaagg cagataagat
    32341 ccctcgtggt aaatggaaca agcatgagag cgaaatcaaa aaagcgttgg attttgatcg
    32401 caatgacacg tttattctct tttcttccgt tacaaaacag ggaaaagaag aagcgtgggc
    32461 agcaatcgaa caagcaattg aatcataaaa agaataaaaa agacttacca gtcaaactct
    32521 ttcataaaag tttggctggt aagtcttttt ttgagcttaa cttacttttg tttcttgtct
    32581 tttttctttt cattttgtgg ctgtggtttt agaaatcgat cgatctcttc ttttggtgca
    32641 actgtttttt ccggatcgac agcaaatgat tcgaaaagtt tatctaaatt atctgtacgt
    32701 tcaaatttca accccattgt aaacacctct ttgtttcttt ttacttagtc tatcatgtaa
    32761 tcgtccaaat tggtttctaa gacataaagt tttagttttt ctttatcgtt ggttataatt
    32821 aaattataaa aagattttaa agaggtgaga aaatgattgg cgaagataaa ttattgatga
    32881 ctatccagct cacagactta aatgaagaag aaaaaaaaca ggaaataagc gattgggcta
    32941 ttttgacacg tctgttgatc cagccaacat ttatccgctc tttcacacaa caagcggatc
    33001 cgtatgatct tcgaaagctt caagaaaaaa taatgctagc atcggtaaga gacgaaagtt
    33061 ggttagttgt aggaaatgac gagaacgaat gtagttttcg cttggaagat aatcaattat
    33121 tgataaaaaa tgtattgtct atctctgttt tcaaagaaaa gcaatttttg attcgagact
    33181 tcattcagaa aaaaatgatt gctcatgggg tgttcgccta tatgcgagct tatagcgagt
    33241 ttatctatca caatacaaaa cagatctctc aacggctatt gtttgagaaa aaggatgaaa
    33301 tcgaacattt acctaaaatg aaacaacaaa acggggaagt agtagtcgat tgcaaccaat
    33361 ttccaggata cgatcttttt taccaaggtt tatgtttcac ttcgtgttgg gaaatgtatt
    33421 atagtcgcta ttatcatcaa atcatcccta aaccgatttt tctcgacatc caacaagtag
    33481 aaaaagtgaa ggaattagaa aacgaagtca tatgtatcca gctttatcga gaccccttta
    33541 actggcaaaa gccaaacaat caaatgtttc aatcctattt tagggaccag cttgggtttg
    33601 atcatcttgc ttgggacaat ggtgtcggtt tattgaaacc gccattcgtt gaatacattt
    33661 acactgacca tacgatccaa tctgtccagt atcagaatgc acaaatgcaa cctgttccta
    33721 aaaagaatgc ttcatttttt gtaacaaaaa gttatgatat tcaacaagga gattataaag
    33781 aaaggcgtgt tcgcggaaca ctaaatgctc aggcgtattt tccttgggtt gacgagaatc
    33841 gctcgcgcat gatgtgctat aaagttatcg atccgactgt tgctttggat aatggaatcg
    33901 aagcttattg ttattatatt agggaatatc tagaagtaga ggtaacagat gaaaaatatc
    33961 aagagtatct tttatcctta agaatctatg ttccttcaga atccctgtca gaattaccgt
    34021 taaaggaaat caaacataga ctctctgatg ttaccttcaa acgaataaag aaaaagcggc
    34081 gaagtatcca attcgatgtc aagaaaggtg agcagcatct gcgtgtgcaa tttttagatt
    34141 accgagaatt agaacaattg attactttac aaaaaatatg aggtgaagaa aatggataaa
    34201 caactctatt atcgaataat g
//
LOCUS       DAYYNQ010000020        33915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000020 DAYYNQ010000000
VERSION     DAYYNQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.020"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..221)
                     /locus_tag="ACHBJ1_000871"
     CDS             complement(102..221)
                     /locus_tag="ACHBJ1_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM9429837.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            400..2061
                     /locus_tag="ACHBJ1_000872"
     CDS             400..2061
                     /locus_tag="ACHBJ1_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9429838.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2131..2493)
                     /locus_tag="ACHBJ1_000873"
     CDS             complement(2131..2493)
                     /locus_tag="ACHBJ1_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM9429839.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2561..3502
                     /gene="opp3b"
                     /locus_tag="ACHBJ1_000874"
     CDS             2561..3502
                     /gene="opp3b"
                     /locus_tag="ACHBJ1_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9429840.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3503..4546
                     /locus_tag="ACHBJ1_000875"
     CDS             3503..4546
                     /locus_tag="ACHBJ1_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429841.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4552..5622
                     /locus_tag="ACHBJ1_000876"
     CDS             4552..5622
                     /locus_tag="ACHBJ1_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429842.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5612..6565
                     /locus_tag="ACHBJ1_000877"
     CDS             5612..6565
                     /locus_tag="ACHBJ1_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429843.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6577..7587
                     /locus_tag="ACHBJ1_000878"
     CDS             6577..7587
                     /locus_tag="ACHBJ1_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM9429844.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7872..8693)
                     /locus_tag="ACHBJ1_000879"
     CDS             complement(7872..8693)
                     /locus_tag="ACHBJ1_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM9429845.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8790..9068)
                     /locus_tag="ACHBJ1_000880"
     CDS             complement(8790..9068)
                     /locus_tag="ACHBJ1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429846.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9336..11345
                     /gene="metG"
                     /locus_tag="ACHBJ1_000881"
     CDS             9336..11345
                     /gene="metG"
                     /locus_tag="ACHBJ1_000881"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM9429847.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11824..12411)
                     /locus_tag="ACHBJ1_000882"
     CDS             complement(11824..12411)
                     /locus_tag="ACHBJ1_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429848.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12586..13908
                     /locus_tag="ACHBJ1_000883"
     CDS             12586..13908
                     /locus_tag="ACHBJ1_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9429849.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14063..14839
                     /locus_tag="ACHBJ1_000884"
     CDS             14063..14839
                     /locus_tag="ACHBJ1_000884"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9429850.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14832..15401
                     /gene="rnmV"
                     /locus_tag="ACHBJ1_000885"
     CDS             14832..15401
                     /gene="rnmV"
                     /locus_tag="ACHBJ1_000885"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM9429851.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15394..16278
                     /gene="rsmA"
                     /locus_tag="ACHBJ1_000886"
     CDS             15394..16278
                     /gene="rsmA"
                     /locus_tag="ACHBJ1_000886"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM9429852.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16729..17358
                     /locus_tag="ACHBJ1_000887"
     CDS             16729..17358
                     /locus_tag="ACHBJ1_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429853.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17482..18591)
                     /locus_tag="ACHBJ1_000888"
     CDS             complement(17482..18591)
                     /locus_tag="ACHBJ1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429854.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18800..19222
                     /gene="mgsA"
                     /locus_tag="ACHBJ1_000889"
     CDS             18800..19222
                     /gene="mgsA"
                     /locus_tag="ACHBJ1_000889"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM9429855.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19409..19507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19545..20081
                     /locus_tag="ACHBJ1_000890"
     CDS             19545..20081
                     /locus_tag="ACHBJ1_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM9429856.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20121..21884)
                     /locus_tag="ACHBJ1_000891"
     CDS             complement(20121..21884)
                     /locus_tag="ACHBJ1_000891"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429857.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21887..23602)
                     /locus_tag="ACHBJ1_000892"
     CDS             complement(21887..23602)
                     /locus_tag="ACHBJ1_000892"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429858.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24381..24605)
                     /locus_tag="ACHBJ1_000893"
     CDS             complement(24381..24605)
                     /locus_tag="ACHBJ1_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429859.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24796..27573
                     /locus_tag="ACHBJ1_000894"
     CDS             24796..27573
                     /locus_tag="ACHBJ1_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9429860.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27654..28073
                     /locus_tag="ACHBJ1_000895"
     CDS             27654..28073
                     /locus_tag="ACHBJ1_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9429861.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28085..28555
                     /locus_tag="ACHBJ1_000896"
     CDS             28085..28555
                     /locus_tag="ACHBJ1_000896"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9429862.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28572..29351
                     /locus_tag="ACHBJ1_000897"
     CDS             28572..29351
                     /locus_tag="ACHBJ1_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9429863.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29338..30159
                     /locus_tag="ACHBJ1_000898"
     CDS             29338..30159
                     /locus_tag="ACHBJ1_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9429864.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30176..31225
                     /locus_tag="ACHBJ1_000899"
     CDS             30176..31225
                     /locus_tag="ACHBJ1_000899"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9429865.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31238..32242
                     /locus_tag="ACHBJ1_000900"
     CDS             31238..32242
                     /locus_tag="ACHBJ1_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9429866.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32875..33894
                     /locus_tag="ACHBJ1_000901"
     CDS             32875..33894
                     /locus_tag="ACHBJ1_000901"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9429867.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11072 a         6006 c         6925 g         9912 t
ORIGIN      
        1 cttagtttcg agtagtaaga aaaattttgt gaaaatggct ttctacattt ttgcataaat
       61 ttggcttaat accgagaaac tggcttttga acaccgttta ttcagataag ggactgtgac
      121 aagacttttg tcacagtttc tttttgttta ttctttttta tctttgtatg gcaataatag
      181 acaatataac gaaaattatt taatggcttt tggaagacca tggaaaacct tgtacaaagc
      241 tattttttct gaaaaattcc ttgaaacctc acgaaaaaat cattatttta tgaaaaagac
      301 aaaaaaaagt gttgattttt taataattat tcattaaact cttaaacaac ttcagaaaat
      361 attaattatt ctgaaaattc caataaaggg gtacgtaaaa tgaaaaagaa atttacattt
      421 ggtttggttg cggtatgcgg ccttgtactt gctggatgtt atggcggcag ttcagccaca
      481 agcagtagtt cagcaaaaag cggttcagca gatggaggag gcgtatttaa cttagtcgtg
      541 cctcaagaaa tgcctacagc tgacttatca gttgcaacag atacaatcag ttttacagca
      601 ttgaataacg tttatgaagg aatttaccgt cttgatgaag acagcaaacc acagccggca
      661 ggagctagcg agttggcaga agtcagtgaa gacggattaa cttacaaaat caaattacga
      721 gaagacgcaa aatggtcaaa tggtgatcct gttacagcag cggattacgt ttatggctgg
      781 caacgaacag tagacccagc tacagcttct gaatatgctt acctgtatgc gccagttgaa
      841 aatgcagaag acattacagc tggaaagaaa gataaatcag aactagggat caaagcagtt
      901 ggtgactatg aattagaaat caaattgacg aaacaaacac catatttcca atatctgctt
      961 gctttcccat catttttccc acaaaaacaa tccgtagtgg aagaaaatgg ggatgcctat
     1021 gcatctgcaa gtgataaagc tgtttataat ggaccattca cattagaaga ttttgatgga
     1081 ccaggaacag atacagaatg gacttataag aaaaatgatc attactggga caaagataca
     1141 gtaaaacttt ctgaaatcaa agtcagtgta gtaaaagaat cttctactgc attgaactta
     1201 ttcaaagatg gccaagcaga tgacgtgatc ttgtctggtg agttagcaca gcaaaacgca
     1261 aacgatccag cttatacttc tgttaaagaa gcgcggacaa gctatatcga attcaatcaa
     1321 cgtgaaaaga attcaccatt caacaatgtg aacctaagaa aagctatttc ttattctatc
     1381 gatcgtgaag ctttagtaaa acaagtacta ggtgacggat cagttgtctc tacaggattg
     1441 atcccatcag atatggtaaa aagtccaaca acgaacgaag actttgcaaa agaagctgga
     1501 aaattagttt cttatgacaa agataaagca aaagaatatt gggaaaaagc gaaaaaagaa
     1561 ttgggcgttg attctttaga atttgatttg atggcttctg atgatgattc atcgaaaaaa
     1621 gtaatcgaat atgttcaaaa ctcaatccaa gaaaacttgg atggcgtaac agtcaaacca
     1681 acacctgttc cattctctgt acgtcttgac cgctcaactt cgggtgattt tgacgcagta
     1741 ctaggcggat gggctgctga ctatgcagac ccaagcagct ttactgattt gtttgtcaca
     1801 ggtaattcat acaaccgtgg tcgctggagc aatgcagaat atgacgaagc agttaaagca
     1861 gcttcatcaa cagacgctgg cgatgaacaa gcccgttggg atgatttcca aaaagcaatc
     1921 aagatcatct ctgatgatat gggtgtcgtt ccggtgtacc aaaaagcaga aggtcaccta
     1981 gtgaatgaaa aaatcaaagg tatcgttcac catgcggcag gcgcttcatg ggattacaaa
     2041 tggacatacg ttgaagaata atcgttaata ccgtgagcat gagaaaaaac gaggctgcgg
     2101 caaatgtcgc aacctcgttc tcatattcac ttatcctaac aaagaaaaaa gcatgaaaat
     2161 agcagaaaga gcaattagta ttccggcaat gatcagccaa aagctgtaca taccgtagct
     2221 ggcagacgag agcgtggaaa attctggttt tttctttcgg ttccttgttc gtgcaagatg
     2281 gaccgatcgc tgaaaatgat cgaagaaacc cgaagaaaag acccataaaa aaccgccgat
     2341 aatcagaaac ggcaatgacc ataagaatag atcattagat aattgaatga gtgtaaagga
     2401 atggttgatg agatttttta ttaaaataac cagaatcagt agaaaagcta ctgcgtaggg
     2461 gatagatttt ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc
     2521 gaaacatctg tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata
     2581 tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt
     2641 tttcttgatg caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga
     2701 aacaatcgag atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg
     2761 gatttatctt tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca
     2821 gccagtcgca catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc
     2881 aattatcttt ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa
     2941 tacatgggca gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt
     3001 tgtatttgct gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc
     3061 aaaatgggat ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct
     3121 tgctgactct gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt
     3181 ggaattagca aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg
     3241 aaatagtttg attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg
     3301 atcactggtg gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc
     3361 aatcacaaca aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct
     3421 gatttttgtt attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt
     3481 ttcagaaggg agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga
     3541 tatccctgcg gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat
     3601 tactgcacct tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc
     3661 tgccgttatt tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt
     3721 tgtttctcct catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat
     3781 tccgggaatc aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga
     3841 taaatatgct caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt
     3901 gggacgtgat gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt
     3961 tattgctgca ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt
     4021 aggcggacgt gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa
     4081 cctcgttgtc atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc
     4141 tgctatggcc atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa
     4201 attaaaagat caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat
     4261 tgcattcaaa catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag
     4321 tattccatct gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc
     4381 tccgtcagct tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc
     4441 ttatctatta tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact
     4501 tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa
     4561 attttagaag tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg
     4621 attcgcggtg tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct
     4681 ggaagtggaa aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat
     4741 atagatgctg gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag
     4801 atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta
     4861 gatccgacga tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt
     4921 tcaaaaaaag agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa
     4981 gctgagaaac gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc
     5041 gtgatcgcga ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca
     5101 gcgcttgatg tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa
     5161 atcgatacat caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat
     5221 cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc
     5281 tacaacccgc aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt
     5341 gcgaacgatc gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa
     5401 ggagacgcat tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca
     5461 ccattttttg aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca
     5521 cccaaagtga caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa
     5581 aaacatactt acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg
     5641 attgaaacag tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag
     5701 ctttcacatt tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc
     5761 cactactggc cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga
     5821 cggtcaagat ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt
     5881 tcagatgatc tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat
     5941 cattgccgaa ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa
     6001 agtcgatgat ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca
     6061 tgaattttca ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc
     6121 gcgttttatc atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt
     6181 ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca
     6241 tgatttgtcg atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt
     6301 attagagatg ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag
     6361 tttgttatct gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa
     6421 atatcaacct gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca
     6481 ttttgtctat gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa
     6541 ggaatctctt ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt
     6601 atgtatccga cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt
     6661 acctataacc atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat
     6721 ttttcgctca cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt
     6781 cttgctttag caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga
     6841 cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac
     6901 aaccaaaccc gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa
     6961 ctaatcaaga taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct
     7021 gctcgatata atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa
     7081 gaatctttag ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct
     7141 cgacctctag aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca
     7201 cagcggaaat tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag
     7261 gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac
     7321 cagcgagaag attatcttga acaattatct gggaaattgg tcaaacaata tgactactta
     7381 tttgtagaat cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat
     7441 aaattaataa caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc
     7501 aataccgacg gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg
     7561 ggcagacatt cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt
     7621 agccttgtta ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa
     7681 agctcactaa ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt
     7741 ctgctattgt taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa
     7801 aacgtaactc acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt
     7861 tgctcacaac cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg
     7921 attcctacag gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg
     7981 gaatgatcga tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc
     8041 gtaatcgtga aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg
     8101 actacacggt tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg
     8161 ccagaagact ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg
     8221 atcttgttac tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg
     8281 attagcggtt cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga
     8341 tctcgattag gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca
     8401 ccagtttctt cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca
     8461 aagccaccag gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa
     8521 actttcagct gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag
     8581 ggcttgtcat attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt
     8641 gctttatgtt cataatataa tttttcttct tctttagaag caaaacggtt catctgacca
     8701 cctccattga cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa
     8761 ggatttactt gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga
     8821 ttgaataaaa agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca
     8881 aagaagccac aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc
     8941 ataaaactgt tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag
     9001 ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc
     9061 ttctgcatga ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga
     9121 ggggagaaat aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat
     9181 tcaacctatt catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc
     9241 tttttcattt tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac
     9301 gaattcgcat catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata
     9361 tcactacgcc aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga
     9421 ttgcatgtga cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca
     9481 ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac
     9541 cgcaagaata tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata
     9601 tcagttatga taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa
     9661 tatttgatcg tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact
     9721 ctgtttctga cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg
     9781 aaggtaacgt gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag
     9841 aatcatactt cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac
     9901 atcccgaatt catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac
     9961 caggattaga ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga
    10021 gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac
    10081 taggatatgg ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga
    10141 tggtcggtaa agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc
    10201 ttgatttgcc tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa
    10261 aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt
    10321 tagatgcact ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta
    10381 cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat
    10441 tgaatcgaac gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct
    10501 cacttgtcac tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt
    10561 accatgatgc aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga
    10621 tttctcgtgc aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag
    10681 aaaagaaagc tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag
    10741 cgatattact tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat
    10801 tggactctga aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa
    10861 aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt
    10921 ttattcaaga aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc
    10981 cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa
    11041 aagtagaact aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt
    11101 tgctgcaatt ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg
    11161 ctgaatttta tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc
    11221 tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac
    11281 cagatggctc tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg
    11341 catagaaatt caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg
    11401 agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca
    11461 gagaactatc caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat
    11521 ttagaataat actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg
    11581 gcagatttta attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg
    11641 aatgcaatga ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta
    11701 taagttctat cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata
    11761 gataaaaaaa caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc
    11821 ctatcacttg attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc
    11881 agtaagcagt gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata
    11941 atgttgatag agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc
    12001 agctataaac caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg
    12061 atagaaataa aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat
    12121 attcaataaa aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa
    12181 aaaggaatac aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc
    12241 gatgaacaaa gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa
    12301 taaaattaat gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac
    12361 ccggttggat aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc
    12421 ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt
    12481 aagagggagt ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt
    12541 tatactgaaa attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta
    12601 aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca
    12661 ggcgtcttct tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg
    12721 ctagcttggc ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta
    12781 gctacagcaa tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa
    12841 ttgcccagct ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct
    12901 gcattatcga ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat
    12961 cttttgttga ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca
    13021 aaagtcggtg cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg
    13081 attgtcatat ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa
    13141 gctggaaaag atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt
    13201 gcttatgacg gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa
    13261 gatttgccta aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc
    13321 aattttgttt ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct
    13381 tctgaagcat cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc
    13441 ttgatctcgg tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat
    13501 gcgatggctt tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag
    13561 tttacggttc cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc
    13621 cttggttctt tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta
    13681 ctgattttta tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat
    13741 aaagtaccac tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta
    13801 ggtatgacga tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata
    13861 ggtatcccag tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca
    13921 acagacagaa aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa
    13981 aacaagttct tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta
    14041 agaatgaaaa ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca
    14101 gttcaatgaa gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat
    14161 ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta
    14221 tccaaatata tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga
    14281 aatcgaaaat aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat
    14341 tggcttggat tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag
    14401 acagattgct atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact
    14461 tgaagacacg tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca
    14521 tagtttcagt ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc
    14581 atttagcggt gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt
    14641 accaatcgaa cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag
    14701 aggaaagcga aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg
    14761 gaatcttccg acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg
    14821 tctaagcgaa catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg
    14881 atacaagaag gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa
    14941 tcaatgaaga gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt
    15001 ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc
    15061 aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg
    15121 gtgtcgagca tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag
    15181 acatttccca aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa
    15241 tagctggggc acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt
    15301 acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac
    15361 tcgcccaagc aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca
    15421 ccctctagaa caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga
    15481 caaaattttc tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat
    15541 gaacatacga acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc
    15601 aagcatgcaa aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct
    15661 gatacgatgc aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat
    15721 ttgtcaacag ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat
    15781 ttaccttact atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg
    15841 gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga
    15901 acgaaagctt atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca
    15961 ttcatcgtac caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa
    16021 ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg
    16081 acaaaagcgg catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat
    16141 ggcaaagatg accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat
    16201 ccaaaacgtc gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg
    16261 tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca
    16321 tcaagtaata agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt
    16381 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt
    16441 aactctaagt cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg
    16501 tcttattact tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa
    16561 attttgtcac atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac
    16621 actcattttg tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt
    16681 atactaagtc gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat
    16741 tcagttcgaa aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg
    16801 gtcatgccta aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta
    16861 tttattgcga tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt
    16921 ggtttctttt taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt
    16981 gcgttgattg gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag
    17041 tttacttttg ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct
    17101 ttagcagaaa tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat
    17161 tattctcaag gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac
    17221 agtttagttg attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat
    17281 atccccgtat ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg
    17341 aagaaaacga catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata
    17401 ataaatcctc taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc
    17461 acagctctaa cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa
    17521 gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc
    17581 tcttgcatct acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc
    17641 agctccaagt aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc
    17701 aagggctact tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc
    17761 ttcataacct ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc
    17821 atgtccatct gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga
    17881 tccaataaag ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc
    17941 gatttgttgg ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc
    18001 tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat
    18061 ctcagcacgc atggctacac gtaatttagc atctaaattt gataatggct catccattaa
    18121 gaagactttt gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc
    18181 agataaagca gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt
    18241 gtccacacgt tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat
    18301 attatcaaaa acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc
    18361 acggtcttta ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga
    18421 gatatcttcc agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc
    18481 aacaaaaacg ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt
    18541 ttctgcattg tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc
    18601 cacactttct tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact
    18661 tcaatgtcat gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt
    18721 taataaatat ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat
    18781 atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat
    18841 gatggtgaaa ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac
    18901 cgggacgact gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc
    18961 cggaccgtta ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt
    19021 ggtgattttt cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact
    19081 tatccgttta tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt
    19141 gctactaaga ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga
    19201 caagcatcca ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc
    19261 tatatgtagc tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg
    19321 ataatataaa caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag
    19381 tttctgtggg aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa
    19441 gaggatggga tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt
    19501 cgctgcatga tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt
    19561 tagacgcacg tatgattgct attatgggct tattgattgc attgatggtc actttgtccc
    19621 gactggtagc aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga
    19681 tcatgggcat tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag
    19741 taggaatggc cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta
    19801 ttgaaggagc aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca
    19861 ttctggctac gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg
    19921 cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa
    19981 cagtaatatg gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt
    20041 acaaaaaaat tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca
    20101 tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa
    20161 taaccatctt tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc
    20221 atcactaata tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg
    20281 gtttttcctt ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg
    20341 actgagctgg tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca
    20401 atcgtcaaca gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta
    20461 tctaattttt ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca
    20521 ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg
    20581 tcaaatagcc aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg
    20641 atagagcgga tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc
    20701 atcagcaaat taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt
    20761 tgacctggat aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa
    20821 aaagaaacat gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca
    20881 tctggcaaat caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca
    20941 gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac
    21001 tgacttgagt agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc
    21061 aatgccaata ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga
    21121 gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca
    21181 gcatttagca gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt
    21241 ttctggttac cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt
    21301 ctatcgaaat ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt
    21361 gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa
    21421 agaatgatga ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa
    21481 atattgtcca tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa
    21541 taacgaagtg gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat
    21601 aagtaagcta accgattgct taacacttgg atgagccatt gactgactac gttgattaaa
    21661 acgataccac cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct
    21721 ccaatcattt gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca
    21781 ttgatgattc ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga
    21841 atgaatcggc ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct
    21901 gtgatgcgac gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct
    21961 gagcagcaat cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga
    22021 cacgttggga gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat
    22081 ccaagtctgt ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct
    22141 tggcaatcaa agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc
    22201 cattttccat gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct
    22261 gagcgatttt cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa
    22321 ggttttcacg aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct
    22381 gacggagatg atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg
    22441 aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg
    22501 gaccaatgat tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa
    22561 gaccagaatc aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag
    22621 aaaaatcggg agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct
    22681 ctttcacccg atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa
    22741 cgatcagtgc caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt
    22801 gcaagctacc aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat
    22861 tcatgatcaa ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat
    22921 tgttcgctag atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa
    22981 atgcgcggat gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta
    23041 gtttttcttg cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga
    23101 ttgagaaaat aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag
    23161 ccatgaccac tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg
    23221 ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc
    23281 caaaatgatt caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta
    23341 actctgtacc aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa
    23401 tcagaccaat cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt
    23461 cattcatttt tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga
    23521 agacagcttc aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg
    23581 cataacgtaa taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa
    23641 aacaagtact accacattgt agcttaggaa atttagaatg aaaagagaac aagccttttc
    23701 ataaatgaaa gttttattat gaagaagctt tagaaattga atagactatt ttttttaaaa
    23761 aaaagaaaat gagaaaacat gccagtctct tcattcatag gaacgtctat tttacttaat
    23821 ttttctataa atgaattctg cataaaaaat atatcaagta aatgtttata gatgaaactc
    23881 tatttacttc ttttttaatt tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa
    23941 agtgggttga gagaaatcta attgattttc aaagaaggag tatgaaaatg agaaaaatat
    24001 ttattcgttt attgtgggta ctatcttaga ggaataaata atagtggaga aaactatggt
    24061 ttggcgcaga ttctatcagg tttgatggaa cgaagacaac ctcccgacat caagtcaaaa
    24121 gccacttgag attttgatct gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt
    24181 ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa
    24241 ggatggtgac aacagtacct ctttactctc tgaaagcatc ataactcgtt tttcctcatt
    24301 atgccaataa attgttgtta taccacatat aaggatttgt aatagctaaa aacagacagg
    24361 aaagttgaaa tctgtgaatc ttattttgta tggaaggatt tatagaacgc aaaaatcaag
    24421 ataaagatga ataaaaatcc tgtttcggct atatattgcg tatctacgcc aataaaacga
    24481 gtttgttctg tcatagccat ataaagcaaa ggcaaaaata caactacagc gagataagat
    24541 ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc tgtttttata tttatccgat
    24601 ttcattgaat agtcatagcc tccttattct ttcatatgtt gaaattccag tataaaaact
    24661 ggaaggaact gtacgttttt tagaataaaa attataaggg ataagtaggc tgctgtctat
    24721 aataagatat atttttcaat tctttattaa agattttatt gacaaaatga atccgttttc
    24781 tatgatggag gaggggtgaa taagttgttg aagaaggaaa ttttatgtta cttagagaac
    24841 cagacagcct ttttcgattt agaacatatg aacgaggtct ttacagcaca aaggttagct
    24901 gatcattttg gggtaaaaag aaacacagtc agtcattatt tgaaccagtt aaatgatgag
    24961 caactactag tcaaaatcaa ttctcgacca gtgatttatt tccacaaaga agcttttgaa
    25021 aaacaatttt ttaagttacg cacgaatttt tatttttcta tcgatgatct caaagaggaa
    25081 cagccttttt tcgcgcaacc aaaagactta ttctctttta tgatcggata cgacgccagt
    25141 ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact atccagatgg cggattacca
    25201 cttttgatta caggcgaaag tggcacaggt aaaagttatt tagttaattt ggtctatcag
    25261 tattgtctgc ttcatgatct gctagaagat tctgcaccat tcatcacttt taattgtgca
    25321 caatatgcag ataatcctga acttttgact agcaatttgt ttggacatgt taagggagcg
    25381 tacactggtg cagaagaaaa taaaaaaggg gcttttgaag cagcagatgg tgggatcttg
    25441 tttttagacg aggtacatcg cttaacacca gaaggtcagg aaaaactgtt cacctatctt
    25501 gatcaaggtg tgatttatcg tatgggagaa cctaatttgt cacgccggat caaaacacgt
    25561 ctcttttttg ctacgactga agagattact agtcatttct taacaacttt cattcgaaga
    25621 atacctatcc gaatcgaact acctgcactt aatcagcgta gtcgtaatga acggctagaa
    25681 ctgatttact ctttttttct cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt
    25741 ggaccagctt tgtcactttt aacaagtcag aaattatccg gaaacatagg agagctgaaa
    25801 aatatcgtca aagtttcagc agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc
    25861 catgtcacga tccatcattt gcaaaaagaa ctacttgcgc agcctattac taaaaataca
    25921 gcacagcaag gaatctatat tacgcaagac cagaccttag agcaattaat ggagaagcaa
    25981 cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa
    26041 aataactgtc tgctatattc ttgcgaagga aaattaaagc aagaagtgat tcaattattt
    26101 gattttttgc tgtttgagac cagtagacaa gaaaaacacg agctgctggt ttattggaca
    26161 caatctatcc gagagacctt tcgacaaatg gaaactgcct atcaaatcaa attcgatgga
    26221 aacagtgtct atgccttgag ttactatctc tatcagcgaa gcactatcaa atggttaccg
    26281 gaagaacatg atattatcca actaatcaaa gaattagaac aacaagtttc tcagtcttat
    26341 ccatctagtt atcactatgt ccaacgaatt ctggaattaa gtaagatcaa attagatatt
    26401 gaagacacaa gtatggatcg gattttactg acgatctatc taaaaaaagc gaaatggtca
    26461 aaagaaaaac gactgccaaa agcgatgatt gctgctcacg gttatgcaac tgctagcagt
    26521 atggcaaatg tagtaaatcg tttattacaa gaggatctat ttgaatcatt tgatatgcca
    26581 ctagatgtca caccacagca gattgcttca gaaatccttg attattgcga aaacagcgat
    26641 gtttcaaatg gattgatcat attagtagac atgggatctc tgaaagaaat ccatcagttt
    26701 tttaaaaaac agttatccgt accgcttttg attttgaata atgtgacgac acctttagca
    26761 atcacagtag gggaatgcct gcaaaaaaat gtatcattag aagagatagc agaagagact
    26821 gttcgacaga tccagccaga atggcgactg ctctatccag aggaaaacaa acccaaagca
    26881 ctcattacta cttgcttcac tggaatcggt acggcggtcc acttaagtga actgctagaa
    26941 aaaagcttac ctacggcttg tcagttgaaa atcatcccat atgaatatca gcaattaaaa
    27001 gataagaaaa acagtgaccc attattttcc atatacgaag tcgtaggaat gattggcacc
    27061 acagatcctg atatcaccaa tatcgactat ctttcattag aagacctcat ctcgggggaa
    27121 aagatgtctg tactagctga atggttggac actactatga atgcttcaga gaaggaaata
    27181 ttcaatcagc gcatcattcg taatttttca ttggaaaaag tactggattc cgtgactatt
    27241 ctagatacag agaaggtgat gggagaaatc gatcatttta tgagagaatt agaggtgcag
    27301 ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc
    27361 attgaacgat tgatccgaca aatgccaatc gaaacctatc aaggattaga gaaatggaag
    27421 cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt ttagtgtcat tgaagagcat
    27481 tatagtgtca tgatccccaa ttctgagatt gcctatatct atgatatttt gagtgaagat
    27541 acagagtttc tctcgataga agaagaattt tgaacataag caagttgttg ccactaattt
    27601 tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag aggagatgaa aaagtggata
    27661 gagaaattat cttagcttcc catggaaaat ttgcttcagg gatattagat tcattagagc
    27721 tgatctatgg gaaaaatcat tctattactg tattcgattg ctatactgat gagaattttg
    27781 atttagcgga aacagtaagg cagcttttta tgaggtacaa agataaagaa atcattgttc
    27841 tgacggatct ttttggcgga agtgtcaaca acgaattctt acaatatatc aatcgggatc
    27901 atgtctatct cgttgccgga ttgaatttgc cactattgat tgagctagta tctcggttgg
    27961 acgctgaagt agagacaact gttttgatcc agcaggtgct aactgattca aaaaaaatga
    28021 tccagttttg taacgagcgg ctcaatcaga aaatagaaga agacgaattt taggaggaag
    28081 aaaaatgatt gtattgacaa gagtcgacca tcggttatta cacggacaag tagctttttc
    28141 atggacacaa acaataggcg cagactgtat tttgattgct aatgatgatg tacctacgaa
    28201 tgagatacga aagacaacga tcaaattagc caagccacaa ggagtcaaac tagtgatcaa
    28261 atcaattgat gactcgattg ccgcgttgaa aagcggggtg acagataaat ataagttatt
    28321 catcgtagtg gaatcgatcg aggatgcata taagctggca gaagcgtatc cagaaatcaa
    28381 aaccatcaac ttaggaggga tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt
    28441 aaatatttta ccagaagagg aggaacgctt gaaacgatta attgacaaac aaatagaagt
    28501 agaaatccgt caagtaccag cagataaaaa agtggatgta agagaagttt tgtaagaaag
    28561 gaagaacctc aatgattgta caagcagtct tattaggaat tgttgcattt gtcgcacaat
    28621 cagaatatgc actagggaca tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag
    28681 gaatcgtgtt aggtgatgta aaaactggga tcattatggg cgcaacttta gagttagcat
    28741 ttatcggatc gttctcagta ggtgcttcga ttccacctga tgttgtgacc ggtgggattt
    28801 tagggacggc ttttgctatt acagctggag ccggtacaga aaccgcgctt ttactaggtt
    28861 tgccgattgc tacgttgaca ttgatattga aaaatattta cttagggctt ttgattccaa
    28921 ttatgaacca taaagcagac aattatgcag aagaaggaaa ttataaagga gtcgagcata
    28981 tgcacttgct tgccggtatc ggtctttcct tgatgcttgg tgttgtggta atggtttctt
    29041 atatggtggg aagcaatacg atcggaaatc tattaaatat gattcctgac ttcgttcaaa
    29101 aggggttgtc agtagctaca gggttgattc cagctcttgg ttttgcgatg cttgctcgtt
    29161 tgctgatcaa caaacaagtt gcaccatatt ttttcttagg ttttgctata gcttcttact
    29221 tagagattcc tgtgaccgga gtagctattt ttggtgcgat tttagcagtt gtggtagtca
    29281 atattatgaa tgtccgtcaa ctaccacttc aaacaactgc aggggaggta gaagaagatg
    29341 aagaattctg agatcaaaaa cagcaaaata acgaaaaaag agctaaatca cgtattttgg
    29401 cgttcttttc aaatggagtt ttcttggaat tatgaacgtc agatgaatat ggcttatgtt
    29461 tatgcaatga ttccgatttt gaaaaagtta tatcagtcga aagaagaaat ggcagcagct
    29521 ttgaaacggc atctagagtt ttttaacacg accccacata tcgttacact gattttagga
    29581 atcacggctg caatggagga agaaaataag gatgatcctg aatttgatgt gactgcgatc
    29641 gatagtatca aaacatcttt gatgggacct cttgcagggt taggagattc tttcttttgg
    29701 ggaactttgc gattgattgc cacaggtgtg ggtacctctt tagccatgca aggaaatatt
    29761 ttaggcccta tcttgttcat tttgattttc aatattccgc acattctgtt tcgctatcta
    29821 gcaacaggtt ggggatatcg cttaggaacg ggttttttga aaaagatcca ggcaaacggt
    29881 atgatggaaa gtttgacatt aggcgcgtct attattggac tgatggtcgt tggagcaatg
    29941 actgccacga tgatcaccat caatattcct ttaaaaatcg gttcaggaga aaatgctgtg
    30001 actgttcaaa gtatttttga tgatatcgtt ccaaatatct tatgtcttgg agcatttggc
    30061 atagtgtttt atctattgaa gaaagaagtg aaaccattaa cgatcttgat cggactagct
    30121 ttctttggaa tttttggttc gtggataggg attttttaaa aaaggagcag agaaaatgtt
    30181 tacgatgcaa gattatatct atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa
    30241 tgatttttcg acaagagaac atatgaaaaa aacagtgaat ttactgatct tagcaacggg
    30301 ttcatcttat aacgcttgtt tagcagcgaa gccagcattg gaatcttatg gtgatcttac
    30361 tgtcgatatt caagaacctt tttatttttt caattatgga aaactttctc cttcaatcga
    30421 tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct actgttgaag cagtaaaaat
    30481 gatacaaaaa caagggttac ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc
    30541 tttagaagct gcacaaatta ttgatttagg tgccggggta gaatcagtag gcttcgttac
    30601 aaaaggctat tctgcgactg ttttacagtt actactctta gggataggaa ttggaataag
    30661 taaaaataaa atcagcaaaa aaatagaaca agactacatg caacagttgc gcaaaataat
    30721 caaccattta ccagcgatta ttcaaaaaac ggaagaattt tttgatggat atcaaagttt
    30781 atttcgttta gcacagcgtt tcgtcaccat tggatatggt ccaaactggg gaacagctaa
    30841 agaagcagag acgaaattga ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga
    30901 agcttatatg cacggcccat acctggaggc agatgcttcg catttgctat tctttattga
    30961 aggggaaagt gtaaataagg aacgttcgca aaagttgcaa agatatatga gtcggtacgt
    31021 tggagagact ctcaccatta ccacaaaaaa agcaagaaac gagaaaacac ttggattagc
    31081 aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc gttccgtttc aactattcgc
    31141 ttataaaacc gcagtagcta aaggtattga cttgaacaaa aagatttttg aagactttga
    31201 tacagtcttg aaaagtaaat tataagaggt ggaaatcatg ctgaaattca atgaagaaga
    31261 acaaatcaaa gcaataaaag gcgcactagc tttacgtccg caagtagaag aaatcatcga
    31321 taaactttat gcggaaaaat ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc
    31381 ttcctcgatg caagctgtca cttatatgaa tgggaaaagc aacttgcctg tctttgtaca
    31441 gcatgctgca gaatattaca ctactggaaa caaaagatta acaaaagatt ctatcgttat
    31501 tttgtcctct gttaccggta caactcaaga agtagtgaaa gcagtagaac aaatcaagaa
    31561 agtaggagcg acactgattg gatttatcga taaagcgaac agtaagttgt ctcagctatg
    31621 cgattttgtg gtcacttatc cagctcctgg aacagaacaa atcaaatttt ttatggcagc
    31681 agatcgattg atgtatttga atggagaatt cgaagcttat tcagaatact atgaacagct
    31741 cgaacgttat ttaccaacag ggttagtgga agcagagaaa aaagcagatg catttggctt
    31801 atcatttgca gaaaaacatc gacgtgatag tatgcattat tttattggag cagggaatca
    31861 atggggagct gtttattcat atgccatgtg ttactgggaa gaacaaagct ggcttccatc
    31921 gaaatctatc catgctgccg agtttcttca cgggacgtta gagatcgtag aagaaacaac
    31981 cccagtcaca ttatttttag gcgaagatga acagagagtg ttatctgaac gtgtggctaa
    32041 acttttgcca aaaatttgca gcaattatac attgatagac acaaaagact atccagtaga
    32101 aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc ttattgatgc atgtagtgac
    32161 acaacgcatc gatgctcatg tggagcagtt gaactgccat ccattagaga tacgccgcta
    32221 ctatcgtcag tttgattatt aggatagact ggataaaaag aatataaaaa ataaagagag
    32281 cagcgataac tgctcccttt atttttttaa aatattggca tcaattacgt tataaaacac
    32341 attttttgca ttatctacag ttcaacttat aatctgttta atgggacatg tgggtgccaa
    32401 ccattttttg ttatgcaata gtactattta gttgttgcat ggatccgtag taaaagtcca
    32461 agtacaacga gttgcgccag tttacatagc gttttgtatc cagaatgttg actaaaacaa
    32521 aagttcctat gtcaaccgtc aacagagaca atctgtccgt tgcaggacct gacagattta
    32581 gaaatgttca atctgtctct tatgtatttt aaatcaatac tcactaggag aagtttccta
    32641 acctcctaat agtaatgctg gtagtaacat tgtcaaaact gcttcctctt aggaacaagc
    32701 aaagcataac gagtgtaaaa ataaaatgta gactattgta acatatcatt gattatttca
    32761 agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt
    32821 taagaaatgt atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa
    32881 caaaaaatcg cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca
    32941 gaaaacgggc atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat
    33001 aagtatcata caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga
    33061 tataaagaat tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca
    33121 aaagcgattc gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt
    33181 atacatgcaa gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct
    33241 gaagaaattc cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct
    33301 gaggaagtgg cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca
    33361 gcagcctatg tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat
    33421 gtgattggcg tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct
    33481 atccatggat taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca
    33541 gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt
    33601 ggtgtggggg acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt
    33661 aacttgttag gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt
    33721 gagggtgtgg caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg
    33781 ccaattactc aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca
    33841 aaagaaatca tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat
    33901 gatcaatagt taatc
//
LOCUS       DAYYNQ010000021        32345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000021 DAYYNQ010000000
VERSION     DAYYNQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.021"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1161
                     /locus_tag="ACHBJ1_000902"
     CDS             <1..1161
                     /locus_tag="ACHBJ1_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM9429868.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYK
                     LTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1544..2272)
                     /locus_tag="ACHBJ1_000903"
     CDS             complement(1544..2272)
                     /locus_tag="ACHBJ1_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429869.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2250..3824)
                     /locus_tag="ACHBJ1_000904"
     CDS             complement(2250..3824)
                     /locus_tag="ACHBJ1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9429870.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4053..4991
                     /locus_tag="ACHBJ1_000905"
     CDS             4053..4991
                     /locus_tag="ACHBJ1_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429871.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5003..5914
                     /locus_tag="ACHBJ1_000906"
     CDS             5003..5914
                     /locus_tag="ACHBJ1_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429872.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            5938..7410
                     /locus_tag="ACHBJ1_000907"
     CDS             5938..7410
                     /locus_tag="ACHBJ1_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9429873.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7461..8072
                     /locus_tag="ACHBJ1_000908"
     CDS             7461..8072
                     /locus_tag="ACHBJ1_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM9429874.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8102..10015
                     /locus_tag="ACHBJ1_000909"
     CDS             8102..10015
                     /locus_tag="ACHBJ1_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM9429875.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10143..13124
                     /locus_tag="ACHBJ1_000910"
     CDS             10143..13124
                     /locus_tag="ACHBJ1_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM9429876.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13121..14782
                     /locus_tag="ACHBJ1_000911"
     CDS             13121..14782
                     /locus_tag="ACHBJ1_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM9429877.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14822..15754
                     /locus_tag="ACHBJ1_000912"
     CDS             14822..15754
                     /locus_tag="ACHBJ1_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM9429878.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(16146..17693)
                     /locus_tag="ACHBJ1_000913"
     CDS             complement(16146..17693)
                     /locus_tag="ACHBJ1_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM9429879.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(17795..18148)
                     /gene="tnpB"
                     /locus_tag="ACHBJ1_000914"
     CDS             complement(17795..18148)
                     /gene="tnpB"
                     /locus_tag="ACHBJ1_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM9429880.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(18138..18332)
                     /locus_tag="ACHBJ1_000915"
     CDS             complement(18138..18332)
                     /locus_tag="ACHBJ1_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429881.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(18558..19760)
                     /locus_tag="ACHBJ1_000916"
     CDS             complement(18558..19760)
                     /locus_tag="ACHBJ1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429882.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(19763..19957)
                     /locus_tag="ACHBJ1_000917"
     CDS             complement(19763..19957)
                     /locus_tag="ACHBJ1_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9429883.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(20004..20852)
                     /locus_tag="ACHBJ1_000918"
     CDS             complement(20004..20852)
                     /locus_tag="ACHBJ1_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM9429884.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(21057..21407)
                     /locus_tag="ACHBJ1_000919"
     CDS             complement(21057..21407)
                     /locus_tag="ACHBJ1_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429885.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            21585..22448
                     /locus_tag="ACHBJ1_000920"
     CDS             21585..22448
                     /locus_tag="ACHBJ1_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9429886.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            23072..24064
                     /locus_tag="ACHBJ1_000921"
     CDS             23072..24064
                     /locus_tag="ACHBJ1_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429887.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            24080..24964
                     /locus_tag="ACHBJ1_000922"
     CDS             24080..24964
                     /locus_tag="ACHBJ1_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9429888.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            25013..26620
                     /locus_tag="ACHBJ1_000923"
     CDS             25013..26620
                     /locus_tag="ACHBJ1_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9429889.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            26621..28105
                     /locus_tag="ACHBJ1_000924"
     CDS             26621..28105
                     /locus_tag="ACHBJ1_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM9429890.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            28282..29274
                     /locus_tag="ACHBJ1_000925"
     CDS             28282..29274
                     /locus_tag="ACHBJ1_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9429891.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            29326..30195
                     /locus_tag="ACHBJ1_000926"
     CDS             29326..30195
                     /locus_tag="ACHBJ1_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9429892.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(30615..32228)
                     /gene="guaA"
                     /locus_tag="ACHBJ1_000927"
     CDS             complement(30615..32228)
                     /gene="guaA"
                     /locus_tag="ACHBJ1_000927"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9429893.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(32235..>32345)
                     /locus_tag="ACHBJ1_000928"
     CDS             complement(32235..>32345)
                     /locus_tag="ACHBJ1_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9429894.1"
                     /translation="GLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT        10131 a         5455 c         6512 g        10247 t
ORIGIN      
        1 aaattggatg actctccttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatattg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcaattct catggaactg
      241 tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg
      301 acacggatct tgcgtgaagc aacgaagcat taccagtcac gtatgaactt ccttccgacg
      361 gtgttgtgta tggatgagtt caaatcaatg aagtctgttt ctggttccat gagttttatt
      421 tgtgttgatg ggacaaccca tcagctattt acgatccttg aagatcgcag actttacaaa
      481 ctaactcaat acttcctgca ctttcctaga aaagcccgtt taaaggtcaa gtacctagtg
      541 atggacatga atgccagcta ttgtcagttg cttaaaacag tctttccaaa tgcggagatc
      601 gtaactgacc gctttcatat tgtccaacac atcaatcgca gcttcaatca attacgtgtc
      661 cagatcatga atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc
      721 aagcgatatt ggaaactctt actcaaagat tccacgacat gggagccact caaacggcat
      781 tatcatcgac tgtttaaacg accaattagt caaacagaaa tcgttgatga gctgttaagc
      841 tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat tattacgcta ttatttcgtt
      901 aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag
      961 tcgtttaaaa agaaattcac gatcttccat tgctaccaat cgggtatttc aaatgccttt
     1021 aaggtgtccc actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa
     1081 cgaattgcgt ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa
     1141 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
     1201 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagcctat ttaaataact
     1261 acatccattt attctatctt ctaaaatagc ctttttcatt tcagttaaat agttcagcgc
     1321 catttttgca ctcgacttgc atagtagttt tttccatatt cctcaacctc agcgggggtt
     1381 gaagacataa ggaaaatcct atgcgttatg ctcaactgga tctgattaaa tattctaatg
     1441 tatttttctc taccatcatt aaatggttaa tagccaaaaa tcaccacttt tcaatggctc
     1501 cttgttgtat aagtataact agaagatgaa cacgtctgag aaattataga agctttttac
     1561 ggtaattact ggggctgtgt ccagtagttt ttttgaagag aacagaaaaa tagctcacgt
     1621 gatctattcc tactttttga ctaatttcat caatagataa gaatgtgtct ttcagtagag
     1681 cacttgctct tttgattcga aagtctagca agtaatcatg aaaggaataa cctgtttctt
     1741 ttttgaaggc ccgtcctaaa taagcgggat ggtaatggaa ttgtgtagct acatctttga
     1801 gcgtaagctt ttcactataa ttgttttgaa cgtaatcaat cacttgttga attgactcat
     1861 taaaagaatc atcagttaga gagttttgat tagctgctga tttacgcatt ttttcttctt
     1921 gtactaactt aactaaataa ggaaaatcaa tgggtttcgt tatgtaactt ttaatagata
     1981 aatcaatccc cttttttgca tactgaaaat tttggtgggc actaatgaca atactagtaa
     2041 agtagcgatt ggatgtttgt tgcaaagatt cgattaattc gagtccagaa attttcccca
     2101 tcgtaatatc tgtgataagt aaatcaaagc gctggttttg caggttttta aatgcttcgt
     2161 ctccgtctgt gtagcagtga tcgataatga tgtcggggac tttcttcaaa agataatgcc
     2221 ttatgccttc tacaattgta caatcatcgt caacaattgc aattctcaac atgcatattc
     2281 cccccttttt gtattttgat agtaatcgtt gttccgacgt gtagttggct ttctataaca
     2341 aaacgtgtat ctccaggata gagatgcttt atacgtcgaa aagaatttga gatgccgata
     2401 ttctcactat aaggaatatc atcattaagt ttttgttgaa tatgctccag cttgtcttgg
     2461 tccattcctt tcccattttc aacgattttt aacacataat ggttggttgt ttcagttgca
     2521 tagacagtca aacgattttc cttttttgat gaatcgaagc cgtgtttaat ataattttca
     2581 acaaatggct gtaaagaaag ttttggtaac attaattgat caatatgatc aatcagctga
     2641 tagtcaaagt tatgtcgata acgacgttga tgtagttgca aggagaggtc gatattaatt
     2701 aattcctctt tcaaatcaat tatgtcgggc tgatatgtat tattgcgata caacttgctc
     2761 aataaatata tactgtcgct gatttcttcg tcatcattca atatggcagt cattcgaata
     2821 aattccaaat tattaaatag aaaatgaggc tcgatttgat attgaagtgc tgcatacttt
     2881 gcttcttgct tcaaccaatt attttcataa agtgtttttt gatatgtagc attatctaaa
     2941 taaaactgat tgatatgcat agcgatttct ttcaaatcac cgactttgtt ttcgatattg
     3001 attgacttgt agtctcctgt agatgaatga tttaaggaag taatgatttc ctgatactgc
     3061 tggatatagt tacgaaaact tctatttaat aattgatgaa tagcaagaat aaacaaactc
     3121 attaataaga aaatcatgat tgccagacct aataaggttt gtgacgaata atttggattt
     3181 tcatatactt gtaatgtgtg gttaccgata gctatctgtt gcgttttttt ccatttagaa
     3241 ggcgagatgt cttcagataa attgacggaa tcaataccat tagacaaaga ataattgtaa
     3301 tgtgttggaa agagactctt ttttagcgcg ttgatcgaaa cgtctagaat caatgtagca
     3361 tcgggcatgt cctcactaat gaaacttaat gaacgaataa aagaaatttt ttcgtttttt
     3421 gtaggtgcgt aggcacttaa gtatttttcg taatctaatg agatagatat atttttaact
     3481 tcatcatctt gcacaagttt ttttaagtta tctactaaat aggtagggga gcgatccttt
     3541 gacaaaatat cttccatgta ttcagcaggt gatgagaggt agtagtcatt aaggttttct
     3601 tggaaatcat aattttgata gatctctgtc acgataccgg agagaatacg attatatgtt
     3661 tccatatagt gggtgatttg ctgaatgttt tgcaggtttt gatttttttc tttttcaatc
     3721 acattgatat aggaaaagaa caatgtgaaa caggcaagta aagtaataaa aaaaatcaat
     3781 atgtatgtaa aacgcataat aacttttgat aaatcgtttt tcattcaaag tcctcatttt
     3841 gataatctct aattttatga atcattatag cacatattac aagtgatgta aacgctttgc
     3901 aaacagggct acttatgatt gtgaatacag tttccgttta aaaactattc tattgtagag
     3961 gtttaataaa ctaaaaataa tgtataaaaa attaatgtaa gcgattaacc aagtgggtat
     4021 aatcaaggaa acgctgagag aggagagaca aaatgaagct aaagaaaggg ttattaggaa
     4081 atctaaaaaa aaaccgagca ttgtttatta tgcttttacc aggtgcgtta tggttattta
     4141 tattttgcta tataccaatg tttggagttg taatcgcttt taaagatttc agaatttcat
     4201 caaggggatt ctttacaagt ttgttcaaca gtccttgggt tggctgggaa aatttcaaat
     4261 atctctttgc ttcgcaagat gcttggatca ttactcgcaa tacggttgtt tataacgttg
     4321 taggtatttt gctaagtact ttttgtacag tgtcattagc aatcattctg agccaaatgg
     4381 cgaacaaacg gttattgaaa aagatgcaga catttatgat ttttcctaat tttttatcat
     4441 gggtagtcat cagttacttt gtttacgcat ttttgagtac ggataaagga ttaattaata
     4501 ttttactaag caattttggt gtggaaccca taaattggta tagtgaaccg aagtactggc
     4561 cttttattat tgtttttacg agtttatgga aaggcgtagg ctatggatcc atcatttatt
     4621 acgcgtcaat tatggggatc gatactagtt attatgaagc tgctttagtt gatggtgcca
     4681 gtaaatggca acagataagg aatgtagtct tgccacattt aagatcgatt attgtcatta
     4741 tgttgatttt gtctgttggt agtatttttc gagctgattt tggattgttt tatcaattgc
     4801 caagaaattc gggggctctt tatgatgtga cgaatgtatt agatacttat gtctaccgtg
     4861 gattaatgaa tagcggagat atcggaatgt ctacggcagc gggattatat cagtcatcag
     4921 ttggctttgt cttgattttg attactaata agatagtcaa caagttagat cctagttcag
     4981 cactatttta ggaggaatca aaatgatatc aaaattaaaa cggcagatgt acttttcgct
     5041 gccagtcgaa cggtttttca caatattact aatcttattt actcttagct gtatttttcc
     5101 ctttattttc gtaatcgtca tttcattaac agatgaaaaa gcattacaac tcaacggtta
     5161 cgaattattg ccggctcaat ggagcactga tgcatatcaa tacttaatac aagatggcgg
     5221 acagttactt cggtcattag gcgttacaat tatgattaca gtgattggaa cgttgattac
     5281 tgtttttatg acgggaacct atgcttatgt tttatcaaga gcctctttcc cttaccggaa
     5341 gttctttact ttttatcttt tttttaccat gttatttgcg ggagggatgg ttccttctta
     5401 cttagtgatg acgaagatgc ttggcttaaa aaatacaatt tgggctttga ttctacctct
     5461 tgcttttagc ccatacaatg tcattatttt aaggacattc tttaagaagt cgattccaga
     5521 atcgattatc gaatccgcta aaatggatgg ctgtagtgag tttcgagttt ttttccaaat
     5581 cgttttgcct ttagcgatcc caggggttgc aacaataggt ttattttcaa gcttaggtta
     5641 ttggaatgac tggttcaacg cattgctgta tattgacacc aacaaattga ttccattgca
     5701 atatttgtta atgaagattc aaaattcaat ggaattctta gcaaataata atgacattac
     5761 attagcgcag caacaagcaa ttcaaaatag ccttcctcaa gaatcaacac gaatggcaat
     5821 ggtcgtagta gctactttac cgattgcaat tgtttatccg ttcttccaaa aatactttgt
     5881 tcaaggattg acgatcggtg gagtgaaaga ataaaaaaga ataggagatg ttaaaagatg
     5941 aaaaagaaca tgcagaaata tttgattggt ggtgcttctg tactaaccct tggcctatta
     6001 ggtgcttgtg gtagcagtaa tggcgaagca aataatgaaa caggaaccac aaaagttact
     6061 tggtatcagg ttggagaaga acccaaagat tttgatgagg tcatgacaaa agtcaacgaa
     6121 aaattaaagg aagaaatcaa tgtagagttg gatatgcggc tgattccgga cggggattat
     6181 cagcaaaagc ttggtgtgat gatcaattca ggagaagaat atgatatttg tttcgtcaat
     6241 ggcacagatt atgtgaatta tgggaacaaa ggtgctttca ttagtctcaa cgatcatttg
     6301 gataaagagt tgaaagatta tgcagctgaa ttgaatgaag gatttattga aggtggggca
     6361 attaatggcg aaacttatgg attgccagtc aataacgata ttgccacaac ggaatattgg
     6421 acctttaata aagatttggt agatcaatac caattggatg tagagaatgt tgaaggtgta
     6481 gaaacattgg ctccagtatt agaaaagttt gcagaaaaaa atactgatac gaatattacg
     6541 ccaatcggta tgacccgtac cagtgtctgt attccttatg acaatattat cggtggtggt
     6601 gtaccgctag cggttgcatt tgaaggcgat acacaaacag ttgttaatac ttatgagact
     6661 ccagaaatga tgacttattt gaaggctatg catgagtttt ataaaaaagg atatattttg
     6721 aaagatgcag ccactgttac gacagatcca tttcgaattg atagtaaaaa ctggttttta
     6781 ggtcgtaact caagtggtta tcaaccatta gcacaaaaaa aattagaagc tctagcggga
     6841 catggattaa ttttaaaacc agctggaaaa gcttttaaaa cgactggatt tgcaagagtg
     6901 gcaatgcatg cggtatctgc ttcaagtaaa catccggaag cagccttgaa attgttgaat
     6961 tatgtgaata ccaatcaaga agtagcggat ttaatggttt atggaattga aggaaaacac
     7021 tatgatcgca atgaagatgg ttcgattact ttaacaaact ctgatgatta ttatcaaggc
     7081 gcttgggcat tggtaaatac ttatctagtt tcaaaaactt caccgattac agaagaaagt
     7141 aaaaaactag aagaagaaag taaaaagttt caaaatgaag caccgatgtc tcctatttta
     7201 ggtttctctt ttgacgcttc tgattttcga acagaaattg caagtgtaca aaatatcgtg
     7261 tcacaatatg aagtactttt aagctcggga acagttgacc ctgaacaata tgtaccagag
     7321 ttgttagaaa aactagaaaa agctggattg gataaaattc aggcatctgc tcaagaacaa
     7381 tacgatgaat ggcgaaaatc taacaaataa ttttttacta aatgaaaaga atagactaag
     7441 cagttagtgg ggggattcaa atgaacgaaa acaaatggac caaaggattt aattgggttt
     7501 ttcaattgtt gttactaaac tttacttatt ttatactaat ttgtttagga tttggaattt
     7561 taggtttttt ccctgctacg atagctttga ttgctggcgt tgtgaatcga aaaaaatatg
     7621 gcttgcaaga gtctgtgaag ttcatgagta aaatctataa gaaaaatttt gttagatata
     7681 atctatattg ctggtcagca actttgttgt tggccggcaa tattactacg atctggcttt
     7741 gttggcagca aagcgcacca ttgttttatt tattaagtac attattatcg gtaattacac
     7801 tgttcctagt ctttgcaatg ttgaattggg tcgtgatcaa aagtcttggg ttgtggatga
     7861 tgggagaccg actgattgac tatttgcttt tcacagcaac gatgttgcca acggtagtca
     7921 tgcaaggctt ggtgcttgga atttatttcc gaatattgat gattttgcct gtgatttttc
     7981 tgtttggcgg aacagctgta acggtaatat tgattagtaa attaactgaa agagcgttag
     8041 caaagttgcc ttgtcagata caatcgcact aattttttca caaaaaggag tatttctttc
     8101 tatgaagtta actgataaac aagtagctaa tatagccttt atccagcaac ttgagattcc
     8161 attgacacaa aaagatgaag aacaaataga cttgattttt actgacacaa aggctaatgt
     8221 gcgacaaatc aagattacta aaaatgagca gcaacgaatt tgcttgtctt attcacatga
     8281 agacttgctg tatcgaggat tgtttatctg ccttccacgt tttttagctc aaggaggaga
     8341 gatgccatac attgaagaaa cctcgatcat tgagaccagt tattcattag atgcttctcg
     8401 taatggcgtg atgaataaag agactctaaa aaaattcatt tcttacttag cgttgatggg
     8461 gtacaacaca ttgtatattt acacagaaga tacctatgaa gtagaagacc ttccatattt
     8521 tggttacctt cgcggagcgt atacaaaaga agaagtgaat gaaattgttg aatttgcttc
     8581 tggttttggc atagaagtag ttccatcgat ccagaccctt gctcacttga cccaattttt
     8641 gaagtggtat gctcaacaag aactgatgga tgatcaaaat actctactga tcggtgaaga
     8701 aaaggtttat gcggcaatcg aaaagatgat tattgcctgc aaagaaatgt atcagacaga
     8761 gcgtattcat ttgggaatgg atgaagccta tcaagcaggt ctgggacgat atttaagcct
     8821 gtatgggtat tgtgatcgga caactttaat gattcaacat atggaccgtg tgttatcaat
     8881 tgttgataaa tataagatga aaccaatgat ttggagtgat ttcatttata agctattaga
     8941 tgaaaccggt aaagataggt attacaatcc tgattttaaa ctcaaagcat tagcagctaa
     9001 aggattacca gcaggattaa cctatgttca ttgggactat ggttcagatg aagtttcctc
     9061 ctatgagaaa gtaattgata accatttgaa attttgtgac attgatcatt acgttttagc
     9121 tagtggtgcc catatctggg ggagactagc cccgaatcat gggaaaacag aagcaattgt
     9181 ttctgcagct gtaaaagctt ctaaaaataa aggtgtacgt tctgtgatgt taactacttg
     9241 gggagatgaa gggcaggaaa cagagcattg gcattcctta attggggcat taaaattagc
     9301 agattatgtc tattctgaag aagagtggaa tgattttgag gaaatctacg atcgtttatt
     9361 aggaacaggt agttatcaat taatgtccga attacgatat ttcgatgaag tggataccat
     9421 tacaaataac aacttacaaa tggcttccgt gagtaaactg ctactttggc aagatccgtt
     9481 agttggtata tatgactttc atgtagccgc tcataacaaa attgccacta gaacaattgg
     9541 tacttattat catgagttag ctcagaaatt agatatgaat aaaaattgga atccaaaaca
     9601 accaatttta acaactattc gtcaacgata tatactttta gctgaaattt tagcacaaaa
     9661 ggcagaacta ggcatctatt tacaacactg ctgtctggta gaagaccttg caggattaat
     9721 ggaattagcg aatcggtgtg aagcactagc caatcgagta aatgaattgg catctattca
     9781 tgaaataatt tggtatcaaa catacaaaac ctatggttgg gaagtattag aacaacgata
     9841 tgctgcatta gctcatcgtc tgaaaaccgt tgcgcgtcgt attaaaaatc atcttcaggg
     9901 gcaagatgcc ctagaggagc ttttggaaga acgattaccg ttttgtcaat atcaagtgcc
     9961 gcttgaaatt tctggcttta attatcggca aagctcggtg tcaggctaca attaacgaag
    10021 gaatcttccc gttgaacgga ccacttgctc atctaatggg aaagtggttc gttttctatt
    10081 cataagtagc ttaccactaa ctcttttatt aagaagagaa aaaaagtagg aagtgagaaa
    10141 atatgttatc ggactattat caaaatctgt cagttataag tgtcaataca gaaccattac
    10201 gtaattatta tattccttat gcaagtaaag aagaagcaaa acaagagtta gaaagggaaa
    10261 aaacgagcca gtttatcgat ctcaatggta catgggattt tcagtattat ccttctgtcc
    10321 aacagttacc ggaaaatttt tttcaagagg cctttactga aagctatgat agaatttctg
    10381 ttcctggagt ttggcaaaca gagggatttg acttacctca gtatatcaat gtatcttatc
    10441 caattccttt tgatccgcct tatgtaccta atgatacccc ttgtggtgta tatcggcgtc
    10501 agtttcaaat tgagcaaaca gatgcacgct tttacttaaa tctagaagga gcagatagta
    10561 attgttttgt ttggataaat agaaactttg taggctatga ccaagtgagt catagtacat
    10621 ctgaatttga tgtgaccaag tatttacagg ttggggttaa tgaaattcaa attctagtga
    10681 tgaagtggtg tgatggtacc tactttgaag atcaggataa gtggcgaatg agcggtctat
    10741 ttcgtccagt gtatcttctt caacgaccaa ctatgcatct gacaagttat caactacgga
    10801 cgttgactga tcctaaaaaa ggaagcggaa cattaatggt ttcttttcaa cgatttactc
    10861 ctattgaagt atcgttacct atttcttacg aattattcca tcctgatgga cgtctcatag
    10921 cgaaagggga gtgttactcg gaacaacaaa taaatttgga tgttgaagat tgcctttttt
    10981 ggacagcaga aacgcctaaa ttatatacat tacttttaac atgtaatggt gaatatttta
    11041 aagaaaaagt tggctttcga gcactgacga tcaagaatcg taagttattg ctgaacggaa
    11101 cacctattaa attatatgga gtcaactatc atgacgtgaa tcagcaaggc acgccagtga
    11161 tgacccgaga gcagttacgt gcagatttgt tattaatgaa aaaattaaat gtgaatactg
    11221 tacgaacggc acattaccct aaatcaccag atttttatga attggcggat gagattggtc
    11281 tgtatgtaat caatgaagca gatttggagt gtcatggtgt ggttaatcta tatgatactg
    11341 aagcgaatta tcaactaatt gctaatgatc cgacattcga aaagccgatt atggaccgag
    11401 tgatccgttc tattcttcct tatcaaaatc gaagctctat cattatgtgg tctatgggga
    11461 atgaatctgg ttatggagta aattttgaaa aagctcaagc tttagcacgg aaattggatg
    11521 attcacggtt gattcataat gaacgctcga ttgaaccagt tattgaccaa gtgaatgatt
    11581 ttagtaactt ggatgttatt agtcaaatgt atcccccact ggaagatgtt catcgtcatt
    11641 gtcaaagaag tgatactgaa aaaccttttt ttatgtgtga atatagccac gctatgggca
    11701 acggtcctgg agatcttaaa acgtacttcg atttgattca acagtatgat tcctttcttg
    11761 gcggttgtgt ctgggagtgg actgatcatg caatcaatag aggaacttta gaaacgccat
    11821 cttatttata cggtggtgat tttggtgaaa aatatcacga cggcaatttt tgcgtagatg
    11881 gtttattgac cccagatcgg caattaacag taggtgcatt ggagtaccgt aatattcatt
    11941 cgcctattca actgattgct gtagatgagg agctgcaaga agtaaccttg agcaatataa
    12001 ctgcttttac agccggttca gcatttaacc tttctcttga atggtatata gatggaaaaa
    12061 aaacagttta tgaagtaatg aatcctttca tacttgagcc tggcaaaagt tgttcattgc
    12121 ccatcacagt acctacaacg attcatggtg ctcatacggt tgctgtttcg ctatctgttt
    12181 ataaaaatga tgagctttat ggacagcaac agctctttct taaaaaagaa aaaatgagat
    12241 tgccaagaac tgctcatcat aatgatgcac cacaaatttc agtaaaaggc acagagctga
    12301 taatcaaagg agaatccttt atttatatat atcaattagc aaaagctgcg ttcacttctg
    12361 taaagatcaa tcaaaaggaa cttttggaac gtcctactca attttcaatt tggcgtgcac
    12421 caacggataa tgatcgtaac ttagtaaaca aatggaaagc agctggatat gctgaaatgt
    12481 ctttagaaat cagaaaaaaa gactggaaat tcgtagatga tcatctcctg atcgaaacgt
    12541 cttatcgagg agttattcca ggattacagc caatttttac aatgcacata cagttgaaaa
    12601 taaatggtgc aggcgtaatc aaccaaaaag tatcagtgga gaaaaatccg atttttccag
    12661 atttccctcg ctttgggatg gactggccgt tgaatgatac tttttcgaag gtttcttatt
    12721 gtggctatgg gccgtatgaa agttatcctg ataagcacta tgctgcgatg tttggtattt
    12781 atgagatgga gattgaggaa tttgatcagc catatatcaa accacaagaa tatggtaacc
    12841 gctggcaatg tgattgggta tcaatcaaaa atcatcaagg atctaccatg acggtttatg
    12901 gaaactctaa tttttctgct ctgccttata gtcaagctca attaaccgaa aagtcacacc
    12961 attatgaatt aataaaagaa aatagaactt ggttcaattt aagttataaa caaaatggta
    13021 tcggctcaca cagttgcggt ccttatttag aagatcgttt ccgttttatg gaagatactt
    13081 ttgactggaa tatagtgatt actttcaaag gagaaaatag atgaaagaaa caaagatcaa
    13141 tcaagtcaaa aaaaaattat ctcgttatca agaagttgtt tatcgaaacc attgcttaaa
    13201 atggtcaaaa aataagctga atgttcgttt taatggatta ttttatcgtt tcgaacagtt
    13261 agaagaagtc tggaaaccgg taacgaattt ccaatcatgt cctgtacagc tcataaatga
    13321 tccaaaagaa acatacgatt tatgtataac acacctttat aaccagcatt tgaaaaatga
    13381 cggattttat ttaaaggtta ctaatgacaa tcaacttacg attgaatcag caaaccttcg
    13441 agggtttcaa tatgctatgg aagccttatt agagagtttt ttttcggtag gagatcaatt
    13501 attattaccg gaattgattc tgaaacatga accatcagtg aaaattcgag gaatcattga
    13561 agggttttat ggtatcccct ggacacatga aatgcgcctc tctctttttt catatatgaa
    13621 agataaccaa ttgaatactt ttatgtatgc ccctaaagat gatgaattat tacgtaaaaa
    13681 atggcgtgaa ctctacgatg cgcaagaatt agacaaattt aaggagcttt tatctgctgc
    13741 tgaggctagc aatatcgatt tttggtactt aattagtcct ggaaatgata ttgatattac
    13801 gtgtgaagaa gacattcaag ttcttctgaa aaagttagaa caactgattg agcttggcgt
    13861 atttcaattt ggtctattaa tggatgatat tgactatcaa ttaaaaggtg ctgctaaacg
    13921 ccgttttcga gagccagcat tcgctcatgc ctatttagtg aatcgagtag aggaatatct
    13981 tagcactcgg ttgtccaatc atgaattagt catttgtcct acagaatatg ataatcgtca
    14041 tggttctcgc tatttagcaa cgttaagtca agaaatacca gaacaattac cgttcttttg
    14101 gactggccca agcacgttag cggcgtcaat ttctacagaa gagctgcagg aaatggcttc
    14161 tgtctatcaa cggccgatgc tgatttggga taatattccg gtaaatgatt atttagagga
    14221 taaagaacta ttgttcatga gtccatatga gaatcgaaca ccgaatcttt ctaaggagag
    14281 ataccaggtt acaggagtag tttcgaatcc aatggctcaa ttagaagctt caaagtttac
    14341 gatcaatagt atggctaatt acttatggaa ctgtgagcgt tttgatcctt tggaaacttg
    14401 gacgtcagtc gttgaaaaag tagttggaag taaactacaa ccagcttatc ttaccttaac
    14461 caatgctttt ccaaatcatt acacaagcag tctgctttca gatgaagaat tactgctagc
    14521 caaagaccca gaatgggtca caaaagaaat ggctgcttta gtccaagcag taaaatggat
    14581 ccagcaagaa caacatacat ctagattgag aacagaattg gagccttggt tacaagcaat
    14641 cacagaaagt tggactttct ggcaagaatg gcaattaaaa aaagatccaa aagaagcaga
    14701 agaatggttg gctaagaaaa aaacacaccg cataggacgt gatttagtaa ctgcccattt
    14761 agagcaaatt atcaaaagtt agatatctta aaaaataaaa agcaagaaaa aggagaacaa
    14821 aatggtcaag agattaatta gtgcaaacta cagtgatgtc aagaaaatga cgtcaaaaga
    14881 gttgaagcaa tccatcaaag cgagtgaagg acgggtgatt gtttgtgaaa cggttgctaa
    14941 tactcttcca caatctggag aaattacgaa tgcagaagtt gcgtcagcat ttggtgcaga
    15001 tttgattctt ttaaatggtt tcgattgtta tcgtcctgca gtttttggac taccaaatgt
    15061 gacgattgag aaggtttttg atctacagca aacgattgaa aacccgattc ctaaattaaa
    15121 agaactagtc ggtcgtcctg tgggaattaa tttagaaccc attgccccta aaacaaatat
    15181 gatgagtgag aaagtagaaa tcagccaagg aagagcaagc tctgttgaaa cgatacaaaa
    15241 agcagaagaa ctaggagtcg attttatttg ctttaccggt aatccaggca ctggagtaac
    15301 caatttagaa attactaaat ccgtaaaaat ggcaaaaaag tatttttcgg gattgattat
    15361 tgctgggaaa atgcatagcg ccggctcaga agagccagtt gtgaataaag aggcgattat
    15421 ttcttatgcg gaagcaggcg cagatatttt actagttcct tcagtagggt ctatcccagg
    15481 ctttttagag caagacctaa ttgaagcggt tcggttggca aaaaaatatg atttgttaac
    15541 gatgtctgca atcggtacta gtcaggaaag tgcccgacca gaaacattgc ggcaattagc
    15601 cattatgaat aaaagttgtg gtgttgattt acaacatatt ggagatgctg gatacggggg
    15661 attggcacca gcagaaaata ttttcgaaac ctcggttgca attagaggaa tgcgacatac
    15721 catcaactgc gttagccgct cgatcaatcg ctaacaaaat taaataaaaa atggttaaat
    15781 tgattattta agtgggaaca tacttgagat atcagctaac ttctgtcact ttaaaaaagg
    15841 gactgtgaca gaagtgaaaa tagacaaaaa atatccgaac aagagactag gatagcagaa
    15901 aaactatcgt tttgcaacga ggaccaactg gaccgtagga gcaattctct tattgttcgg
    15961 atatttcgct tgttcaagga cttatgtctg tagtgccctt ttttggtagg aaaaatggta
    16021 gggaaccata tcatttttaa tgaaaatcaa gcatttcaaa agttgaaaag taagcgcccc
    16081 atagaacagt acctgtacgt caaataaccc gacctataga atagaatcgg gaatgtttgg
    16141 cgtttttagt tacgagaaaa atgttgttgt acatccttgt tccaaggcaa cagttgatct
    16201 aaagtttctg ctgaaagtac gggaagattt ggtatcttat caaatagaaa ctctagatac
    16261 tttacaggat tcaatccgtt aagttctgct gttttatata cactcagaat gattcctgaa
    16321 gttcgtgctc cttcaagact tgtggaatgc aaccaatttt tccgtcctat caccagctct
    16381 ttgatatttc gctccgcacg attatttgtg agttctaaac gaccatcttc aaaaataggt
    16441 tcaaaaccac ttcgtagttt acacgcatat tgaacggctg catccaactt ggattttgca
    16501 acaggatcta tcgttgccat ccactcataa aaagcaataa ataatggacg cagttcttgt
    16561 tgccgacagc ttaatcgctt ctcaggagaa aagtctttcc atttcttttc taggacaaac
    16621 catttatcca attgtttgac tgcttgagct gcgggaatat ccgtgttttt ctttcctttt
    16681 ggaattgctt cgaaaaattt ccgccgaaca tgcgcgaaac aatagacaag tcgtacactt
    16741 tccacggcat tatacccgct atagccgtca cagtgtaaat accctgtaaa ctccttgaga
    16801 aaagttttcg gaacatctcc agcacgactt tcgtcgtggt gataataaac gatgggatgc
    16861 tgactgtctt ttcctgtaca aaactgccag taataggtat ctgttttggc actttcaatc
    16921 acacgataac ttgtttcatc cccgtgtaga atctcttctt gattaagata gactcttaat
    16981 cgttctccta aagggtctaa cgcgttttgc actgctttaa tatgccaatt ggtgatatta
    17041 tctctcgaaa tatccaaccc atgtgtttgt ttccaatagt tctcttgccg ataggcagga
    17101 actttctgac caaatttcat ccggatcgtt tccgccacta aactattgga acctaagcta
    17161 ttagaaatca aaggtttagg aacgatcgct tttttaaaag aagaaagtcc ttctttttcg
    17221 cagcttttac aacaataggt atgttgataa tgggtgagac aatccaacat agcctgatgg
    17281 aaacgaattt cttctcgtac ttttgtccgt ccgatatctt tcatctgtga accgcaatgt
    17341 tcacaaaatt gttcttccaa aggaaggaga tgttctttct ctgttatagg taagtgtttg
    17401 attttgtctg ctttcgttcc tacaactcgt tgatgtcctt ttactggaat aatggtggca
    17461 gctgcttctg gaacacttac agaaatttct tcatcactaa aaagattcaa ttgcccattc
    17521 gtttcctcaa agagagtctc tttagagcgt ccaaataatt tgttggttaa ataattgacc
    17581 ttttcattca atagttggat ttcagttgta agattctgaa tggtttgttg ttgtgcttga
    17641 ttggttttat gggcttcttg aagcaactga agcaacgtat ctgtagtttc caaaattttt
    17701 caccaccttg aatcgaactg attttgttac agaataccac aagaaacgca caagaaatag
    17761 gggttcaaca cattttttgt gttaaccccc cagcttaaaa aactcctttt tttcctggca
    17821 aaatagctcg aggttgatcg atacttaatc cttctaacaa ccatcgaacc tgttggttcg
    17881 ttaaggttcg aacttcgtct ttcgtccgag gccattttaa cttgccgttt tctatccgct
    17941 tataacagag aagaaaacca tcaccgtccc aatagagaag tttgaaacgg tcttgacggt
    18001 tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag ttgatactct tgttgaataa
    18061 gactggcaag accatcaatt ccttttcgca aatctgtttt tccgcacaca atataaatat
    18121 gtttggtttg aagtaaatca taaagcattg gacaacagtt ctttcaaaat agcttgaaga
    18181 atataattgt taataccgtt atagatcaaa acttcagaac cattctctct tttgatgcgc
    18241 atcgctaact tggcagccgc gggtttacct ttttctggtg cactcttttc gtttcctaat
    18301 tggacaggaa caatctcatt taatttatac atagaaaacc tccagacttc ttgatagctc
    18361 aagtatatct tgaggtgcat ttgttagtaa ggtactgttc tatggggcgc ttacgttgaa
    18421 aaacgttgat ttatcggcat tccccatcca tattatattt acgtcttatt cccactcaat
    18481 atgagtagcc tcgtaaagaa gctattatat cagtgctttt tcagcctatt cctcattttg
    18541 gtagggaaat ggtagggtta gatttttact gcttgatttc cattaaagtt tgcaacttta
    18601 gattttgttg cacaggtaaa tttaatgttt cctgtcattt gttctgcaat tccttcatca
    18661 ttacggctcc atagatgagc gtagtgcttt aaagtgattt cagggctaga atggcctaac
    18721 cgttgcgaca cgaccagaat gtctgcgtta aactcattga ttaggtagct aacatgagaa
    18781 tgacgtaatc cttttccttg tattaggggt accttagcta gtttggcata acgctgaata
    18841 attcgttgaa cagttgaacg atagagtggg aggtcagtgt agctaagaat aaattgttct
    18901 acaccatgat ttttttgaat aactttccac ttttttagca cttcaattgt atcatcgtcc
    18961 agagaaatga tccgcaagcc gctttcagtt tttgtgtaag gtttccgaat aaaatctttt
    19021 tgatttgtca tttccaaggt gtgatgaacc ctcaactttt tacgtttgaa gtccacgtca
    19081 ttccaattaa gtgctaatcc ttcgcttaca cgaacaccag tcatgtaata tagccagatc
    19141 attaaaaaac acatatgttc gtagaaatca tctttataaa tttttgccaa aacttcttca
    19201 aattcttctt ttgtccagta aggaacgatt gattttcctt ttggaattgc ttttgttctt
    19261 ttggaaatat tctcattcag aaattgcagg gtgaccgcat agtctaatga tttacgaaac
    19321 ataccgtaca taagtgaact gtatgcttgt gagtatccgc tatcgttcaa taaccaaatt
    19381 cgataatttt cacaatctcg cacattgatt tcacgaagct tcttatcacc gaaaaattca
    19441 gtaatctgaa tcaatccaca ctcacgagaa ctccaagtac ttttttgtac gctagctttg
    19501 tagtgtggaa cgtaagactg ctccataaat tctttgtaag ttaaatcata attcgcaaat
    19561 ccatttacaa ccatgtattc attttttata cgcatgactt ctttgtgtgc ttcacgagct
    19621 gatgagaatc gttttcctag agaatctttt gttcctttct tctggatcct ctttccagtc
    19681 acttgatctg ttcctaactc aactagataa gaaaagcgac catttttatc ttgatacaca
    19741 caagggtatt ttgttttcat agttattcct cctgtgaaag aggaacacct ataatttctt
    19801 caattacttg tcggggtact accccaactc ttttattatt ataatatgga tatccctgat
    19861 ttaccattct tgtttttgct tgtcggatta ttgtggtagc ttgatgtgtt ttaaatccta
    19921 gagcctctaa atcttttcga caaattgtat ttttcattgt ataaactcac tttcaaagaa
    19981 aacactatca ttgggtaata gtgttatttc tttatttttg tataatgaac ggtactttct
    20041 tgaaaatcca tcacttgttc aggacttccc aactcattta aagctaaagc attgtaaaaa
    20101 aatggacgtc taactatata ttcgcttgta tatagtatat cttgattcgt ggaatcatag
    20161 ggtttcttta ccatcttttt ggtgacagtt ggtagtttga gattccggga cttgaaaaaa
    20221 gcttttttct tatgctcttt caattccagc tctttatcga aatatttgct gatatagcgc
    20281 ccgcgatttt cgatactgtc tacatcgatc ttgttgatct taacgaaacc ctgaccccaa
    20341 atcgtagtta accggttaaa tgagataaag gggaaatcaa acaagataat atggtagtgg
    20401 attgcaccgc gttgctgctt ttcccacgtg gcgagatact ttaaatttgc ttttttagtt
    20461 ttatagaggt cataattcag tcgtttgata aattttttaa attcattatt tgcatactca
    20521 atgtcttgaa tgttttcttt gaaagtgaga gttaaaaatt tcgtttgatt gtcaaaattc
    20581 gtgtctacaa ggcgggcaat ctcaaatcgc atactttgat aatgtttctg tttgcgtttt
    20641 aaactgtcat attgtccttg agcggataat tcatcatagt tttttctttt ttcaagagta
    20701 atatcatttt cataaatcca agctaatttt tgttttcgtt ccagatttac ttcgttttcg
    20761 atcgttttac gtttgggagt aatgattgct tcataatcat aaatttcaat atacgtggga
    20821 gtttcaatga ttttctgatt ataagcttgc aaaaatcatc actccttgtt gcgtattcga
    20881 ttgtcaaaga accagtcatt ttctcctgat gttgatctat aaatcaagtt aagggggaag
    20941 gcctgcgtag cagcccttct agcctatggg gtgttgggcg gtcttaacgg acagaaacgg
    21001 ctctgtgggc gctgccccgc cgtttcgtcc taaccaccca acactccata gtatagttag
    21061 ttttgtgtag tggtaccttg ttttctggga atcaaagttg ccgtatcagc ggttgccgat
    21121 aacccataac tgacaaattg tccattcgag cgtccccaag gtacaatcac caaattggta
    21181 aatgtaatta tggctaaacc ttgttgggta agttcttccg gcgtgatcac aggatgttca
    21241 gatttgactt tgacactgat tttttcgtag cgaagttttg gtaggataca ttcgtagctc
    21301 caacctagaa tttcgccctc cttttgccag cgagtgacat tgacgacttg gtattctttt
    21361 tcgagtgttt cttttggtaa actgagatcg gtaattttta ttggcatcac aattctcctt
    21421 ttttatacga aagtttatac gatttgatta aaaaatatag atggccatct acgacttgta
    21481 ttatatacga gaacttatac gtagtcaaga gattttctaa aaaaatatat acgatttttt
    21541 atacgagtgt tataatgaaa aaaatgataa ggaggaattt gaatatgttt ggtttagtac
    21601 aacctaataa agtgaaggtg ggccaacgaa tcaaagaaat aaaagacgga atgtctctct
    21661 cttttacaga attagggaat cgattaggtt taaaaaagcc gacgatcagt tcctacgttc
    21721 aaggctatgc gttggctcct aagagtgtga tcaaccaatt atctagtatc agcggaaaac
    21781 ctgtcggctg gttttatttt gggcatgtgg aagaatatat ccgagagtat ttgtttctca
    21841 ttggtcagaa aaaaatagtg gacgattatc ctgaaatcat tgagaaaata aagcaagaat
    21901 tttatacggg cgagttcaaa aatcctgctt gggagaatga gctaggctat cctttggagg
    21961 aatttatcca agaatgtttt gtagaatatt cgagtgaaat catgaaggac tatgtcgtac
    22021 agattacgaa agaggtactt gaagaatcca aaacgctaaa caaactatca tcaaaagcaa
    22081 aagaagaagc tgtcacgatt ctttcatctg ggatcattga ctatatcgag atgagtggtg
    22141 agatcaagta tggagagaaa gatcgcattg cgaaaatggt gaaagaatca ataaaaacct
    22201 ttgatgtgaa acaagacctg acatttaatg attcctattt gattggaaag ttgatcaata
    22261 ttcttggtac agatgaagat acggagctgt ttctttcttc tttatcaatg atatttactg
    22321 ataagcattt ttccactcga tttggtgggg aagaattagt cgaaatattt caatcgatgc
    22381 gaccagcgtt aatcaaactc tatcgggaga aaacaccaga tgaattttat gattggtttg
    22441 agaagtaaaa taagaaaagt ggcttagatt acaagttggt gaaaaaattt ttttcagcat
    22501 aacatcagta atctataaag gggttgttga ttatactgta gaagttaagt actcaagaga
    22561 ttttttgtac gtaaacaaag caaaaaaggg tgcagtttca ctagaaaatg tactcttttt
    22621 tgtgattttt cttagttata gagttttttt gaaactttaa aaatgagaaa acatattgat
    22681 tttaaagagt gtagacatag cttcttttgc taaatagatt aaacttactg gtgagtggtc
    22741 ttgaacatac ttttaaatca cattttactg agttttattg gattttataa ttcattggaa
    22801 acctcgtact taatctgttt ttatctatcg actagaatct gtatatattc cagctatcaa
    22861 tgaggccgca gaaaagggtg cttaaaaata atctgttgtt taaaatatta ttatcagaat
    22921 acttagatac aaaattctgg cacaaagtaa aaaaaatatt gacttaaaaa aatggatatg
    22981 gtaatctata aacgaaaata gtggtaacgt ttccaaaatg catcttcgtt aaatttttat
    23041 ttttttaaac gaaaatggaa acgtttctaa aatgtattgg gaggagattg aattggaaaa
    23101 gacattggga ccaggtggtg agataagggc ggtacaacgg gataaaaaaa atatttcgaa
    23161 gtgggaaaaa ttaaagaaac agttgcggcg tgataagtgg atgtacttgt tgctcttacc
    23221 aggtatcatc atgacgttta tctttaagta tgtgccaatg tacggtgctg tgattgcatt
    23281 taaagactat aatcctatgc taggaatttt ggaaagtcct tgggtaggac tagatcactt
    23341 tagggaattt atcagttcac caaattttac gcttttgctg agcaacacat taaaaattag
    23401 tatttatggt ttattgttag gtttttttcc accaattatt ttagcactga gcttgaatca
    23461 aattatcaat aaaaaaataa aacagaaaat ccagttgatt ctatatgcgc cgaattttat
    23521 ctcagtagtt attattgtgg ggatgctgtt tttgttccta tccgcaaatg gacctattaa
    23581 tgggttagta gaaaaggtta cggggcaacc aattatgttc atgtctaatc cagcttattt
    23641 cagaacgatt tatattttat cgggaatttg gcaaggaatc ggctggtcat ccattcttta
    23701 caccgcaacg ctggcgggag tcagtaccga tttatatgag gcagcaaata tcgatggggc
    23761 aaatatttta caaaagattt ggcatatcga tttgcctgca ttgaggcctg tgatggtgat
    23821 cagcttcatc ttggccgccg gcggtattat gaatgtagga tatgaaaaag cctacttgat
    23881 gcaaacttca atgaacatcc ctgcctcaga aatcatttcc acttatgtat acaaagtcgg
    23941 cttgcaatca ggtgattatg cctactctgc tgctgttggt ctgttcaata tgattattaa
    24001 tgtcgtttta ttactgattg tcaacaatat aaccaagcgg ctaaacgatg gtcaaggctt
    24061 gtaatgaaag gagaaaatta tgaatagtgc tttatcaaaa atcgaccgtc aaatcttatg
    24121 gttgaatcga gtgattgtaa cttttttagt cgtcatcacc attgttccgc tgatttatgt
    24181 actggtagct tcttttatgg acccagctac tttgatcaat aaaggtataa gcttcaatcc
    24241 gaaagattgg acaatacagg gatatcaacg ggtatttgca gatgattcca ttttacgcgg
    24301 ttttgtaaat tcgttgtttt actcttttag cttcagctta ttgactgtat ttattacgat
    24361 gatcactgcg tatcctttgt ctcgacctaa cttggcgggg aaaaagatta ttatgacttt
    24421 ttttgtcatt acaatgttcg tgggtggtgg cttgatacct acctatttat tggtaaagaa
    24481 cttgggcatg ttagatagtg tatgggcgat tatcgtacca ggtgctataa atgtgtggaa
    24541 tattatctta gcccgtacct attttagtgc tttgccaaaa gaactttctg aagctgctac
    24601 tatcgatggc gccaatgact tgcaaatact gttcaaagtc atgcttccat tagcgaaacc
    24661 aattatgttc gtattgtttt tatatgcctt tgttgggcaa tggaactcat actttgatgc
    24721 catgatttac atcaaagatc ccgacttaca gccgttgcaa ctcgtattaa gaaatatttt
    24781 gatccaaaac caaccttcac aagacatggt gggagccaat actgccatgg cagaaatgaa
    24841 acaaattgct gagttgatca aatacgcaac gatcgtcatt tctagtctgc cattaatcat
    24901 aatgtatcct ttcttccaaa aatattttga taaaggtgtt ttagttggtt ctgttaaagg
    24961 gtaagtattg ttcactattt gtatgaataa aaattataaa ggagatttta agatgaaagc
    25021 aaaatggatg ctgccaatcg tttttacttc cgtactaatt ttgggggctt gtggaggctc
    25081 aaaagaaaag agtaaatcaa gccctgatta tgaactagaa aatgtctctt ttccattgaa
    25141 agaaaaagta tctttaaaaa tgatgtctca aagtgcccct ttggctccca atgatcctaa
    25201 tgacaaattg attttccaac gattagaaga agagaccaat ctccatattg attggacaaa
    25261 ctacagtagt gattttgccg aaaaaagaaa tttggatatc gctagtggtg atttaccaga
    25321 tgccatcttc aacgccgctg caggtgacta tgatttatta aactgggcgg aaagtggcgt
    25381 tattgtaccg attgaagatt tgatcgaaga ttatatgcca aatctgaaga aaatttttga
    25441 tgaaaacccc gaataccgac aaatgtctac tgcacctgat ggtcacattt actctatgcc
    25501 ttggattgaa gaattaggcg aaggaaaaga gtcgattcat acagtaaatg ggatggcttg
    25561 gatcaacaag gagtggttgg ataatttggg tcttgaaatg ccgcaaacca ctgatcagtt
    25621 gatgactgtg ttggaggctt tcaagacgca agatccaaat ggtaatggtg aagcagatga
    25681 aattcctttt agctttatta acaatggagg aaacgaagac ttaaaatttt taatgggggc
    25741 attcggtcta ggtgataatg atgaccactt agtcgttgat aacgatggtc aggtacaatt
    25801 tactgctaac aaagaagggt ataaagaagc catcaaatat ttcaatgaaa tgaataataa
    25861 aggtcttatt gataaagagg ctttcgaaca agattggaat acgtatgttg ccaaagggaa
    25921 agaacataag tatggcgtgt actttacttg ggataaagca aatatcacgg gtgcagatga
    25981 tagttatgac gtcttgccag tcttagcggg accagatgga acgaaacatg ttactcgtac
    26041 gaatggcatg ggtttctcaa gagatcgttt cgtaattact agtgcgaata aaaacttgga
    26101 acttacagct aggtggatag ataaaatgta tgaaccgtta cagtctgtcc aaaataactg
    26161 gggaacctac ggtgatgaag cacaggcgaa tatttttgaa ttaaaggatg gtatgttaaa
    26221 acatttgcct ttagagggga cagcaccggg ggaactaaga caaaaaaccg aagctggagg
    26281 accattagca gttcttgatt cttattacgg cacagtcact actatgccag atgatgcgaa
    26341 gtggcgttta gatttgatgc atgacacgta tttgccatat attacagaag acaatatcta
    26401 tccacgtgtc tttttagaaa aagaagaaac agatcgatta gcaaaaatcg aaacggatat
    26461 gtgggattac atctatcgca aaagagcaga gtggatcacc ggcggtaaaa tcgatgaaga
    26521 atgggaagaa taccttgctg aattggaacg tgtaggtttg gaagaatgga ttcaaataaa
    26581 acaaactggc tatgataatt ttttgaaggg agcacaataa gtgttagtag aagaaacggc
    26641 gattttggat aaagcaaatc gatttattga aatgatgcga ggaaaagtta accctcagta
    26701 tcgacaaaac tatcattttt ccgcacccat cgggtggata aatgacccca atgggtttat
    26761 atattacaaa gggcattacc atctcttcta tcaatactat ccttacgata gtcgatgggg
    26821 gcccatgcat tgggggcatg cgcgcagtag tgatttggtc cattgggaag atctaccagt
    26881 agcgttagcc ccagatcaac cttatgacag cggcgggtgt ttctcaggaa gtgcaattga
    26941 aagagacgga aaactatatt tattttatac aggctgtcaa gaaattgatg gtattagcca
    27001 tcagcatcaa tgtttggcgg tctctgaaga tggtattcat tttagcaaat acagtggtaa
    27061 tcctgtggta gcagaaaaac agctaggtga aaatgggcta atacaagaat ttcgtgatcc
    27121 taaagtaatt gttcacaacg atggttacta tatggtagta gctactaaat ctacacagtc
    27181 tactggtaga atagtgatgt ttcaatcgtc agatttgtat acttggcact tttactctgt
    27241 gctgctagaa gggacttcag agcaaggaat aatgtgggaa tgtcctgatt tattccattt
    27301 agacggacga gatgtcttaa tcatgtctcc gatacaaatc aagaagaacg gactggaata
    27361 tcataatatc agttctacca tggcttgtat tggtgaggta aactgggaaa caggtcagat
    27421 gatagttgat aatttccatg aaatggattt tggaatggat ttttatgcac cacaaacaac
    27481 aagagatcct aatggtggac gggtgatgat tgcttggatg cagatgtggg atcgtacatt
    27541 gcctacacat gagttaggcc atctttgggc aggctctatg agtttgcccc gagagttaag
    27601 agtgaaaaaa aatcgattgg tacaaaaacc catctcaact gtatatagtc atttagttta
    27661 tcaacaaggc agtgaaaata ttgcagtgac tgacacatca gtcgtatttc gaaacgcgct
    27721 gtctgattct tcatatttac atctagtcgc agatttggag aaagctgaag tttttacaat
    27781 tgaattactg cggtctaaga cggattgtct caaactagcc tatgacactg ctacacaggt
    27841 ttttgaattg gatcgtcaga aactaggaag agagcttaca ggggcggaag aagaccctct
    27901 gaatgtacga aaagttgaag tacctttgat tgataatcag ctcgttcttg agatttttag
    27961 agatacctca tcaattgaag tctttattaa tgggttagaa gttatgacgg ctactttcta
    28021 tgagctggaa aaagctcgag atatcttgtt tcattcgatc ggagaaagtc gtattcaagc
    28081 ttttgaaaca gcgagtgtga aataaatgtt tgattgctta ggcgatgaat caattattta
    28141 ataggttttg aaggataatg tttttagaaa agaacagata gctagatttc ccatggattt
    28201 gctattagta ttttgaaaaa attagtaatt tatgacaaaa ttcattatac taaacttgtg
    28261 cagaaatgtg agggatcaac tatgaaagca acaatgaaag atgtcgcaaa attagcaggt
    28321 gttggagtag gaactgtttc ccgggtattg aataacggtt ctgttaaagc ttctactaga
    28381 gaaaaagttg aagcagcaat gacagagtta aactatcaac cagattttta tgcccggggg
    28441 ttaaagatga agcatacgaa cacggttgct ttgattctac ccacggtatg gcatccattt
    28501 tttggcgaat ttgcctacca tgtagaaaca gcattagaaa gacaaaacta caagctttat
    28561 ttgtgtaact ccagtggtaa tagtgaaaaa gaacaagaat atatccagat ggtcagccaa
    28621 aataaagtag acggcatcat tggaattact tattcagata tcgatgaata catttcgagt
    28681 aatctgcctt ttgttagtat tgaccgttat ttcactgaag aggtgattta tgtgaccgct
    28741 gacaatgcgg ccggtggacg tttggctgca aatgaacttt atcgccgagg atgtcagaat
    28801 gtagcatata tcggtgggta ccaagaaaca ccgaatgaaa cgaagagtcg tcgaaaatat
    28861 tttgaaatcg agtgccgtca actggggatt tcctataaag cgttagatat gttagaacct
    28921 ttagaccatc tctcagaacg tgtaacaaat tttttgttag agaatcccga tatcgatggg
    28981 atttttacga tcaacgatcc aatggcatta gctgtcattg atatgcttga aaaaataggg
    29041 aaaaacgtac cagaggatgt ccaagttatc ggatttgatg gtcaaaagat gacagaagga
    29101 gatccttatc tagtctcaac gattgcccaa cctacaaagc aaatggcgga agtggcagtg
    29161 gcaaaattaa ttgctgtcat taatggagag acagtagaaa aacgtaccgt attgccgatt
    29221 gaatttggcg aaggtgacac aacgaaacct ttagtgagaa acgattttgc ttagtgcaaa
    29281 tacgtttctt tttttgaaaa tgagtgaatg aaaggtgtag ataaaatgtt atatggtgga
    29341 atagaagccg gtggaacaaa gtttgtctgt gggattggta atgaacaatt acaagtgata
    29401 gaacggaaaa gttttcctac cacaacacca gaagaaacga tggagcaagt gtttgatttc
    29461 tttgataagt acaaaggaaa tatcgcatca attggtgtag gagcatttgg gcctattgat
    29521 attcaaatcg aatcaagaac ttacggatat atcaccaaca caccgaagtt agcttggcaa
    29581 aattatcatt ttgtaggagc tttggaaaac gccttgtcgg ttcccatcta ttggactacc
    29641 gatgtaaatg ctgcttgtta tggagaatat atggcagggt atggaaaagg taaaaatagt
    29701 gttctgtatt acaccgttgg tacaggtgtt ggtggtggtg ctgtagtgga tgggaaaata
    29761 gtcgcaggtt ttagtcatcc tgaaatggga catatgctct tacaacgtca taatgaagat
    29821 caatttgaag gaatctgtcc ttttcatcat gattgtttgg aagggttagc atcaggacct
    29881 gcgattgaaa aacgctttgg aagaaaagcc cagactatct catttgatca tccttattgg
    29941 acgattgagg cagattatct tgctcagtgt gtgtataaca caacgttact atatgcaccg
    30001 gaagtaataa ttttaggcgg gggtgtaatg aaacagccgc aactactcga gttgattcgc
    30061 caatcgttta aagaaaaaat gggttcctac gtggatacgc ctactctcga agaatatcta
    30121 ctaactccaa ggcttgctga caatgcgggc ttgattggct gcctagcttt agcaataaag
    30181 aaactcaatc attagaatag tatttattag aactattcat aacataccgg aagtttaaaa
    30241 gggtattctg aaacttaaat agtctcaatt cattcttctt tcttgagaat ctgagtagag
    30301 acttttattc tgtttcaaat taatataatt acgaaatgat attgagtaca agaaagttct
    30361 ttttaccaga tcttttaaat cgaaaaatct gatatatata tttatagtac taaaaatctg
    30421 attagataaa aagctaattc aataggaaaa taaaggtagt gaataaaagc aaagcccttg
    30481 ttagtagtta atctaacaag ggctttggta gggaaaatgg taggaaacca tcttattttt
    30541 tacgaaaatc aggaacttta gtaattgaat aatgttgatt tataggtatt cctcatccat
    30601 attatgttgg ctctttattc ccactccact gttgcaggtg gtttgctggt aatatcatac
    30661 acgatacggt taacgtgtgc gacttcattt acgatacgga cactgatttt ttgcaagact
    30721 tcccaaggga tacgggcaaa gtcagcggtc atgccgtcaa tagaagtgac agcacggata
    30781 cctacagtgt agtcatacgt acggccatct cccataaccc caacagagcg aatgcctggt
    30841 aggacggtga agtattgcca gatgtcgcga tctaaaccgg cattggcgat ttcttcgcgc
    30901 aagatagcgt ctgattcacg gacgatttct aatttttctt ctgtcaattc accaaggaca
    30961 cgaataccta agcctggtcc agggaatggt tggcgccaaa cgatgctgtc aggcatgcct
    31021 aattgggtac ctaattcacg tacttcgtct ttgaacaatg tgttcaatgg ctcgatcagc
    31081 ttgaattgca tgtcttcagg taatccacct acattgtgat gtgatttgat ggtttgggca
    31141 gtttcagttc ctgattcgat cacatcggta taaagggttc cttgtgcaag gaaagaaacg
    31201 ccttcttcac cggcaagttt ggttgcttca tcatcgaaga catatacaaa ttcatttcca
    31261 atgattttac gtttttcttc tggatcagac acgccagcaa gttttgataa gaaacgttct
    31321 ttggcatcga cttttataat gttcaagcca aattttccgc caagactttc cataacttgt
    31381 tctgcttcac ctttacgcaa caatacgtga tcaacgaaga tacatgttaa ttgatcgccg
    31441 atcgcttttt gtaaaagaac accaacaaca gaggaatcta ctccgcctga aaggcctaat
    31501 aaaacttttt tgtcaccgac ttgttcacga atttttgcga tctccatgtc gatgaagtta
    31561 tccattgtcc agtcaccagt acattggcag acatctagtg taaaatggcg caacaagtca
    31621 tttccatatt cggaatggcg aacttctgca tggaattgga ttccgtacat tttgcgttct
    31681 gcattttgga tcgatgcgat ggggcaatct ttgctggtag caacgacatc gaaatcagtc
    31741 ggtgcttggg taactaggtc accatgactc atccaaacgg tttgtttttc aggagtgttt
    31801 tggaaaaggg ccgcttctgc agaagtgatt tccaattctg cttttccgta ttcacggttt
    31861 ttggctggtt taacttttcc gcctaattta taagtaatca actgcatacc gtaacagatt
    31921 cctaaaactg ggatccccaa atcaaaaatc ttttcatcaa taccaaaggc cttctcatca
    31981 taaacactgt ttggtccgcc agaaaggatg attcctttag ggttcattgc ctttacttct
    32041 tctgcggtaa tgcggtggct catcaactct gagaaaacac cgaactcacg aatacggcga
    32101 gtgatcagtt ggttatactg actaccgtaa tcaagtacga taatttttgg ctcttcgtca
    32161 aatcgtgttg gtgaagacaa atttgaataa aacttagtcc gatatgcggc gcgttctgtg
    32221 cgccgcatat ttttttagtt ttcaaagatc agaccttgtt gtaaatagat ccgtgcccga
    32281 aagttataaa agttgcgata accaaacgca attcgtttaa tcagcttgat cttgttattc
    32341 agccc
//
LOCUS       DAYYNQ010000022        30580 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000022 DAYYNQ010000000
VERSION     DAYYNQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.022"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACHBJ1_000929"
     CDS             147..1016
                     /locus_tag="ACHBJ1_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9429895.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACHBJ1_000930"
     CDS             1225..2250
                     /locus_tag="ACHBJ1_000930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9429896.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACHBJ1_000931"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACHBJ1_000931"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM9429897.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="ACHBJ1_000932"
     CDS             3333..5036
                     /locus_tag="ACHBJ1_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9429898.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACHBJ1_000933"
     CDS             5038..7071
                     /locus_tag="ACHBJ1_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM9429899.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACHBJ1_000934"
     CDS             7082..7522
                     /locus_tag="ACHBJ1_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9429900.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACHBJ1_000935"
     CDS             complement(7722..8849)
                     /locus_tag="ACHBJ1_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429901.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACHBJ1_000936"
     CDS             complement(8852..9529)
                     /locus_tag="ACHBJ1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9429902.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACHBJ1_000937"
     CDS             9793..11559
                     /locus_tag="ACHBJ1_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM9429903.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACHBJ1_000938"
     CDS             11574..12011
                     /locus_tag="ACHBJ1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9429904.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACHBJ1_000939"
     CDS             12024..13175
                     /locus_tag="ACHBJ1_000939"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM9429905.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="ACHBJ1_000940"
     CDS             complement(13237..13935)
                     /locus_tag="ACHBJ1_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM9429906.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="ACHBJ1_000941"
     CDS             complement(13928..15280)
                     /locus_tag="ACHBJ1_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM9429907.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="ACHBJ1_000942"
     CDS             complement(15503..16903)
                     /locus_tag="ACHBJ1_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM9429908.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="ACHBJ1_000943"
     CDS             17064..17801
                     /locus_tag="ACHBJ1_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM9429909.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="ACHBJ1_000944"
     CDS             17926..18405
                     /locus_tag="ACHBJ1_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM9429910.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="ACHBJ1_000945"
     CDS             18402..19169
                     /locus_tag="ACHBJ1_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM9429911.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="ACHBJ1_000946"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="ACHBJ1_000946"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9429912.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="ACHBJ1_000947"
     CDS             complement(20072..20674)
                     /locus_tag="ACHBJ1_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9429913.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21010
                     /locus_tag="ACHBJ1_000948"
     CDS             20780..21010
                     /locus_tag="ACHBJ1_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429914.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21001..21249
                     /locus_tag="ACHBJ1_000949"
     CDS             21001..21249
                     /locus_tag="ACHBJ1_000949"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9429915.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21355..21939
                     /locus_tag="ACHBJ1_000950"
     CDS             21355..21939
                     /locus_tag="ACHBJ1_000950"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9429916.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="ACHBJ1_000951"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="ACHBJ1_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM9429917.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="ACHBJ1_000952"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="ACHBJ1_000952"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM9429918.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="ACHBJ1_000953"
     CDS             23840..24019
                     /locus_tag="ACHBJ1_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429919.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="ACHBJ1_000954"
     CDS             24041..25066
                     /locus_tag="ACHBJ1_000954"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM9429920.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25257..26501
                     /gene="glyA"
                     /locus_tag="ACHBJ1_000955"
     CDS             25257..26501
                     /gene="glyA"
                     /locus_tag="ACHBJ1_000955"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM9429921.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26654..27601
                     /locus_tag="ACHBJ1_000956"
     CDS             26654..27601
                     /locus_tag="ACHBJ1_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM9429922.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27735..28364
                     /gene="upp"
                     /locus_tag="ACHBJ1_000957"
     CDS             27735..28364
                     /gene="upp"
                     /locus_tag="ACHBJ1_000957"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM9429923.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28503..28745)
                     /locus_tag="ACHBJ1_000958"
     CDS             complement(28503..28745)
                     /locus_tag="ACHBJ1_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429924.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28866..28938
                     /locus_tag="ACHBJ1_000959"
     tRNA            28866..28938
                     /locus_tag="ACHBJ1_000959"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28899..28901,aa:Thr,seq:cgt)
     gene            complement(29057..29695)
                     /locus_tag="ACHBJ1_000960"
     CDS             complement(29057..29695)
                     /locus_tag="ACHBJ1_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM9429925.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29818..30450)
                     /locus_tag="ACHBJ1_000961"
     CDS             complement(29818..30450)
                     /locus_tag="ACHBJ1_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM9429926.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        10412 a         5373 c         6173 g         8622 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatcca gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaattgt aggaaaataa aaaggagctg ttgtttgtgg
    25261 tagattacaa aacgtttgac cctgatcttt gggctgcgat cgccaaagaa gaagaaagac
    25321 aagaacataa tttggagctg attgcctctg aaaactttgt ctccgaagca gtgatggctg
    25381 cacaaggaag tatcttgaca aataaatacg cagaaggtta tccagggcat cgctattatg
    25441 gtgggtgtga atttgtcgat atcgttgaaa atttagcgat tgaccgtgca aaagaattat
    25501 tcggagcgaa atttgcgaac gtccagcctc actccggctc tcaagccaac acggctgctt
    25561 atctcgcttt ggtagaacca ggggacacga tcttaggaat ggatctttcc gcaggcggac
    25621 atctaacaca tggatcacca gtcaacttca gtggcaaaac ctatcacttt gttgcttacg
    25681 gtgttgatcc gacaacagaa gttattgatt ataatgtggt acgtatttta gctagaaaac
    25741 atcaaccaaa attgatcgta gcaggtgcca gtgcttacgg acgaacgatc gactttgcga
    25801 aattcagaga aatcgcagat gaagtaggcg caaaactgat ggtagatatg gcacatatcg
    25861 caggattagt agcagcagga cttcatccta atccagttcc ttatgcagat atcacaacga
    25921 ctaccactca taaaacattg cgtggccctc gaggcggcat gattttgaca aacgatgaag
    25981 cattagcaaa aaaaataaat agtgcggtat ttcctggtat tcaaggcggc cctctagagc
    26041 atgtgattgc gggtaaagca gtagcattca aagaagcatt agatcctgca tttaaagaat
    26101 atagtgaaca aatcatcgcc aatgcaaaag caatggtcaa agttttcaat caagcaattg
    26161 gaaccagagt catttcaggg gcgacagata atcatctgat gttgatcgat gttcgcgagt
    26221 taggtataaa tggaaaagaa gcagagagca ttttggacag tgtgaatatc actgtaaata
    26281 aaaactcgat tccttttgaa acactaagtc cattcaaaac aagcggtatt cgaatcggca
    26341 cgccagcaat cactactcgc gggttcaaag aagaagatgc tgtaaaagtg gctgaactag
    26401 ttgtcaaagc attacaagca aaagatgata acgctcaact agacgaagtc aaaacaggtg
    26461 tacgcgaatt aactgaaaaa tttccgttgc ataaaaaata aaaatttaaa ttccattagt
    26521 aaacagaaaa gttcaatgtt gggcttttct gtttattttt tgggaaaacg gattctttga
    26581 caatatcggt gatgctctct agtatatgag gtgtaatttt agtcttcaca ctaaatctca
    26641 gggaggaaaa gtcatgactg atcgtatctc gttagaaaag gaagcagtag cattcagtga
    26701 tgcaaatgcg cctcatccgc gtatctatga actagcaccg gaagaaggaa gagttttact
    26761 agaagaagta caagaatcac ctgtgaacaa atattccgtc gatattgaag acttgatggt
    26821 ggatactggc gaatggggaa aactcaatat tcgtctcgta cgaccagaag ggaatatgga
    26881 taaattacca gttatttttt atattcatgg agcaggctgg gtttttggca gtgctcaaac
    26941 ccatgataaa ttgatcagag aacttgcggt tcgtacgaat tccgtcgttg tctttccaga
    27001 atatagtcga tcaccagaag caaaatatcc aacagcaatt gaacaaaatt atgctgtgtt
    27061 gcaacagctg aataaattag ccaagtcaaa aaatctggat ctcgagaatc taatcgttgc
    27121 aggagattca gtaggtggta acatggctac agtcatgacg atcttaacaa aagaacgaaa
    27181 gggcctccca atatccaagc aattgctgta ttatccagtc acaaacgctg attttgatac
    27241 agattcctat catgaattcg gagaaaacta ttttttgaca agagaaggaa tgatgtggtt
    27301 ttgggaccaa tatacgacga atgaagaaga gcgtgctgaa atcacagctt cgcctttgag
    27361 agcaaatata aatgaactaa atgatcttcc ttcagcaatg attttaacgg gagaagcgga
    27421 tgtattaaga gatgaaggtg aggcttatgc gcgcaaactt cgtgatgcag gggttcctgt
    27481 tacacaagta cgcttccaag gaatcatcca tgattttgtc atggtaaatg ctatggatca
    27541 gacaaaggca acgcgtgcag caatggcatt atctacttct tggatcaaga aaaaagaata
    27601 agctttgctt gtccgcagga aaacttcttt tcaaaaacgg attttcttag gaaaaatggc
    27661 gagtttgact agtcaatcac ttgattttgt tttacaatta aaaagaaacg acagaacaaa
    27721 ggagagctta aaaaatgggc aaatttcaag tgatcgatca tccattgatt caacacaaac
    27781 taactattat tcgggacaaa aactgcggaa caaaggtttt tcgtgaagta gtcgatgaaa
    27841 tcgctatgtt gatggcatat gaagtatcac gggatatgcc attggaagac atcgtgatcg
    27901 aaacaccgat cactgaaaca acacaaaaaa cgttgtctgg taaaaaagta gcgattattc
    27961 caatcttgcg cgcaggtatt ggaatggttg acggtatctt ggaattgatt ccagctgcta
    28021 aagtgggaca tatcggtctt tatcgagatg aagaaacatt ggaaccacat gaatatttcg
    28081 tcaaacttcc agaagacatt gatgcacgtc agttgtttgt agtagatcca atgttggcaa
    28141 caggcggatc agcaatcatg gcaatcgatg cattgaaaga acgtggcgcg tcgaacatta
    28201 aatttgtttg tttggtagca gcacctgagg gagtaaaagc tttgcaagaa gcgcatcctg
    28261 atattgacat ctatacagct tctctagatg aaaaattgaa tgaacatggt tatatcgttc
    28321 ctggactagg ggatgcgggt gaccgtttgt ttggtacaaa ataagtaaat acgaaaaagg
    28381 gtgtgacaca acttttgtca catccttttt tggtcatttt taacgtttga tgcagaacag
    28441 ctttttcaca gctttcgttt gcatataaaa aaaccatccc aaaaatgcag agatggtcaa
    28501 attcaaatct tttttagttc tttgtagtta acgattacta cacccttact ttgcgtcatc
    28561 aagttcgcat tttctgcagt ttcatcaatc tcaacaacag cggaattttt caattgcttt
    28621 gaaatcgttc cagtttgcga cttgccgtga attgtgaaca ttacgtgagt attgatcttg
    28681 aatttttcac caatttccga tggtacgata gttgtatcaa attttccaaa agttgccatt
    28741 ttcataaaac ctgcctttca catcaattat accacgtttt atgaaaaata tcatgaaatt
    28801 aaactaattt tccatcggac tctttacaat cagccaaata gagtatataa tttcttttgg
    28861 ataaggccag cttagctcag ttggtagagc aacgcactcg taacgcgtag gtcgtaggtt
    28921 cgatccctat agctggcatt aaaagagaag acagatatat caaggatttt taatccattg
    28981 atacatctgt ctttttgttt aaaggaaata aagtatgctt tcttagcaat agagcatgta
    29041 ttatttctat aaaaatttag gagaataaaa cgactagcaa tatagctgtg aaatcaaata
    29101 tcacatgaac tgcccaactt gtaatgatcg agttgctttt aatggcagct tggttgaata
    29161 acaatcttgc caccccttgt atcaaaagga tgtgtgctac agtgtgtaaa atatttccat
    29221 tatcataagt actgtaatgg ataaatccaa agatgattgc tgacaagaaa ctagcgacag
    29281 gaatgctgaa cttactagaa aaaatagcga gaaaatacac tgagaacaat tcttctccaa
    29341 gtatcatgat aggcaaaata ataaataata acgggctgaa agcctcattt attggattgc
    29401 ctgttaaacc ccatttcaaa atttgttgca aaaataccga gactgcggca ctgattacta
    29461 cattgataaa aaaatatttg gctatatttt tgacaggagc gacaggtttc gagaacattt
    29521 ttttcgtatc ttttttacca aaaacaagtg taaatgctat aaatccagtg atagttaaag
    29581 aaaaaatgct aataatggat ggcgcaaata gcaaccctaa caaaccgagt acaacgacaa
    29641 tagtgcccaa tatccctttt ttaagttcta ttttttttcc taaaatgtta ttcatgataa
    29701 taactcctac tattcagttt ttgaagatag agtcagtata catgaagagt taaaataggc
    29761 aatgtttttt tatggatatt tgtattgcta aaaatttatc ttatatattc attttgatta
    29821 tactactttt atttgtaaaa attttctagc aaaaccaagt gcttgcaact gattaagcgt
    29881 acaatttttt agatgttccg agcgtacttc aatcatttca aaggagttgc tacttaaatc
    29941 gatatttttt aaatcagtat caaaaaagct agcattttct aaatcattag tagaaaacat
    30001 tattttagtg agggaacagt ctacaaaatc tgcgcttgat aagacagagt gctcaaaggt
    30061 actttctttt atgtttgaac cagataaatt tacatagtcc attttacaat tctcgaataa
    30121 cgtgttccca atataacttt ccatcaattt agtacctgtt aatttacaat tgacaaattt
    30181 cacgcgatgc aacatgcttt ttgtcaatat acagcctgaa aaatcacagt tttcgaatat
    30241 acagtcggta atctccattc tttgaaaatc attggaagca aaaaatgcat acttgaaaat
    30301 agatttgtaa ataattggat ggcagattat ttggttttcg atgacctctt tttgtgaaag
    30361 gatattatac agttcaaaat cttcgttttc atagatatga aaaaaggatc ctttattcaa
    30421 gcttgcttca ttaactttag gaaaaatcat atttaattac ctccattttc ataaagtcat
    30481 attaacaaaa gctaaaccaa cttacattag cagaaaatga gataggtttt cgcctttttc
    30541 gataacgata gtgtcgctat ctcttgaaac gttcttagaa
//
LOCUS       DAYYNQ010000023        30306 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000023 DAYYNQ010000000
VERSION     DAYYNQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.023"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(352..1296)
                     /locus_tag="ACHBJ1_000962"
     CDS             complement(352..1296)
                     /locus_tag="ACHBJ1_000962"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9429927.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1592..2536)
                     /locus_tag="ACHBJ1_000963"
     CDS             complement(1592..2536)
                     /locus_tag="ACHBJ1_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM9429928.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2757..3971
                     /locus_tag="ACHBJ1_000964"
     CDS             2757..3971
                     /locus_tag="ACHBJ1_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9429929.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3993..6008
                     /locus_tag="ACHBJ1_000965"
     CDS             3993..6008
                     /locus_tag="ACHBJ1_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM9429930.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6091..7464)
                     /locus_tag="ACHBJ1_000966"
     CDS             complement(6091..7464)
                     /locus_tag="ACHBJ1_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9429931.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7490..7774)
                     /locus_tag="ACHBJ1_000967"
     CDS             complement(7490..7774)
                     /locus_tag="ACHBJ1_000967"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9429932.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7776..9791)
                     /locus_tag="ACHBJ1_000968"
     CDS             complement(7776..9791)
                     /locus_tag="ACHBJ1_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9429933.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9970..10275)
                     /locus_tag="ACHBJ1_000969"
     CDS             complement(9970..10275)
                     /locus_tag="ACHBJ1_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM9429934.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10364..11395)
                     /locus_tag="ACHBJ1_000970"
     CDS             complement(10364..11395)
                     /locus_tag="ACHBJ1_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9429935.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11602..12477
                     /locus_tag="ACHBJ1_000971"
     CDS             11602..12477
                     /locus_tag="ACHBJ1_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9429936.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12629..13471)
                     /locus_tag="ACHBJ1_000972"
     CDS             complement(12629..13471)
                     /locus_tag="ACHBJ1_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9429937.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13563..15440)
                     /locus_tag="ACHBJ1_000973"
     CDS             complement(13563..15440)
                     /locus_tag="ACHBJ1_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9429938.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15437..16858)
                     /locus_tag="ACHBJ1_000974"
     CDS             complement(15437..16858)
                     /locus_tag="ACHBJ1_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM9429939.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16861..20250)
                     /locus_tag="ACHBJ1_000975"
     CDS             complement(16861..20250)
                     /locus_tag="ACHBJ1_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9429940.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20507..21898
                     /locus_tag="ACHBJ1_000976"
     CDS             20507..21898
                     /locus_tag="ACHBJ1_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429941.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21971..22618)
                     /locus_tag="ACHBJ1_000977"
     CDS             complement(21971..22618)
                     /locus_tag="ACHBJ1_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9429942.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22626..23312)
                     /locus_tag="ACHBJ1_000978"
     CDS             complement(22626..23312)
                     /locus_tag="ACHBJ1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9429943.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23318..24391)
                     /locus_tag="ACHBJ1_000979"
     CDS             complement(23318..24391)
                     /locus_tag="ACHBJ1_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9429944.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24613..25287
                     /locus_tag="ACHBJ1_000980"
     CDS             24613..25287
                     /locus_tag="ACHBJ1_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9429945.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25292..26632
                     /locus_tag="ACHBJ1_000981"
     CDS             25292..26632
                     /locus_tag="ACHBJ1_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM9429946.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26781..27767)
                     /locus_tag="ACHBJ1_000982"
     CDS             complement(26781..27767)
                     /locus_tag="ACHBJ1_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM9429947.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28491..29522
                     /locus_tag="ACHBJ1_000983"
     CDS             28491..29522
                     /locus_tag="ACHBJ1_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9429948.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29631..30056
                     /locus_tag="ACHBJ1_000984"
                     /pseudo
     CDS             29631..30056
                     /locus_tag="ACHBJ1_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            30169..>30306
                     /locus_tag="ACHBJ1_000985"
     CDS             30169..>30306
                     /locus_tag="ACHBJ1_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM9429949.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT         9015 a         5666 c         5435 g        10190 t
ORIGIN      
        1 aaattataaa aaaattttgc agtttttctt actttttttg tcgtttaatt gaatgataaa
       61 aaccagtttc tttattcagt taatcaaata tgaacgaatt agatatgaat cattgtattt
      121 gccgttgcgg aaggattttt taattaacca cagataaaca ttgataacga ttcgcttatt
      181 gtaatcaaca gattaaattt catatgttca tatgtacgga tacattttcg gaccgattaa
      241 ttttaaccat tctatttatt tgcttaccta ttagatatca ataaaaaact gcttaacagc
      301 tatttcagct gttaagcagt tttaggattt ttagttcata ttcattgttt cttataatac
      361 agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt tttcttcttc
      421 gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg atggcacacc
      481 tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag gcaatacagc
      541 ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa taccatagta
      601 agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat cagcaatcac
      661 atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca ctggtttacc
      721 accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac ctaagatata
      781 gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac ggaaacgagt
      841 tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag attcgttcca
      901 agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac cgtcgaatcc
      961 tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt taactaaatc
     1021 taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga tatcagcatc
     1081 tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc aagccatacc
     1141 atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga tcaatactaa
     1201 ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa caaaacctgt
     1261 accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc ttttcataac
     1321 tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac atgttaatta
     1381 taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat aacagataaa
     1441 tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata
     1501 aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt
     1561 ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag
     1621 tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac
     1681 gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga
     1741 attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac
     1801 taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa
     1861 aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg
     1921 atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga
     1981 gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa
     2041 tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc
     2101 ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat
     2161 gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct
     2221 cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt
     2281 cttccatctc tatcctttct ccattaataa tttttaaaca tgtaccgcta tacatataat
     2341 tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga
     2401 tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat
     2461 ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg
     2521 attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt
     2581 tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt
     2641 tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga
     2701 gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc
     2761 ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg
     2821 ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat
     2881 ctgctgttga attattaacg actgctccct cttttatggt cactattttt gttttgatta
     2941 gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg
     3001 catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa
     3061 gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg
     3121 ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat
     3181 ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata
     3241 cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta
     3301 cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa
     3361 cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg
     3421 ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg
     3481 cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct
     3541 ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg
     3601 gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa
     3661 caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc
     3721 ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt
     3781 tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg
     3841 ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag
     3901 tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga
     3961 caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat
     4021 ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg
     4081 aagaacgagg ttttgactgg ccagcgtact ggtacatgga tgattgccat cggaacgtca
     4141 agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag
     4201 ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag
     4261 ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta
     4321 tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat
     4381 atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt
     4441 atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg
     4501 attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat
     4561 ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact
     4621 ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt
     4681 ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc
     4741 tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat
     4801 cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc
     4861 gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg
     4921 acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc
     4981 atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata
     5041 tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga
     5101 gatacttgat ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga
     5161 tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat
     5221 tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag
     5281 agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc
     5341 tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga
     5401 gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt gctaacaaag
     5461 accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta
     5521 ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg
     5581 tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct
     5641 gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt
     5701 atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt
     5761 ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag
     5821 attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa
     5881 aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg
     5941 gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa
     6001 atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat
     6061 tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt
     6121 tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc
     6181 aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag
     6241 ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat
     6301 aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct
     6361 tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac
     6421 taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc
     6481 tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat
     6541 aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc
     6601 tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt
     6661 gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct
     6721 ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag
     6781 catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc
     6841 tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat
     6901 atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg
     6961 acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat
     7021 ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata
     7081 aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat
     7141 attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc
     7201 aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa
     7261 catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag
     7321 gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag
     7381 aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac
     7441 aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg
     7501 acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta
     7561 cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat
     7621 aatcagccat atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca
     7681 catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc
     7741 ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc
     7801 gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa
     7861 agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa
     7921 cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt
     7981 ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg
     8041 atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg
     8101 atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct
     8161 tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa taattcagat
     8221 agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact
     8281 tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac
     8341 aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga
     8401 ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt
     8461 ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca
     8521 acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc
     8581 tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct
     8641 cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata
     8701 gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt
     8761 aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc
     8821 acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag
     8881 acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga
     8941 tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct
     9001 tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa
     9061 tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt
     9121 tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa
     9181 taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt
     9241 ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac
     9301 gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct
     9361 cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc
     9421 tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct
     9481 ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa
     9541 accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta
     9601 atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta
     9661 tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg
     9721 atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt
     9781 ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat
     9841 cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc
     9901 tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat
     9961 ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt
    10021 gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt
    10081 ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga
    10141 gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa
    10201 agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc
    10261 gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag
    10321 acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg
    10381 attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat
    10441 ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc
    10501 tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat
    10561 aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt
    10621 aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt
    10681 ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa
    10741 ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta
    10801 aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat
    10861 ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat
    10921 tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta
    10981 attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt
    11041 ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct
    11101 ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt
    11161 ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat
    11221 catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg
    11281 gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag
    11341 gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg
    11401 gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat
    11461 agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa
    11521 tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag
    11581 atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt
    11641 accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac
    11701 atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta
    11761 tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt
    11821 catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa
    11881 gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa
    11941 gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca
    12001 cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat
    12061 aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg
    12121 aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc
    12181 catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt
    12241 accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag
    12301 gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta
    12361 ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg
    12421 aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa
    12481 aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat
    12541 aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga
    12601 atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac
    12661 gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga
    12721 aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt gcatgataag
    12781 gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta
    12841 ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta
    12901 ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag
    12961 aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat
    13021 ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata
    13081 atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat
    13141 tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt
    13201 tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta
    13261 attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt
    13321 tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt
    13381 cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca
    13441 gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat
    13501 aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa
    13561 gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact
    13621 actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg
    13681 tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca
    13741 tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct
    13801 tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt
    13861 gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg
    13921 atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca
    13981 agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg
    14041 acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta
    14101 gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat
    14161 gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct
    14221 gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc atataaggca
    14281 taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata
    14341 agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata
    14401 ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc attcaattga
    14461 ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg
    14521 ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca
    14581 ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg
    14641 cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta
    14701 atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt
    14761 tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca
    14821 tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg
    14881 actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag
    14941 ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct
    15001 gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt
    15061 ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta
    15121 ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt
    15181 tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg
    15241 tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca
    15301 gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt
    15361 gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt
    15421 atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg
    15481 caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt
    15541 ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct
    15601 ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat
    15661 aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg
    15721 aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact
    15781 tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg
    15841 cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg
    15901 tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt
    15961 tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt
    16021 tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt
    16081 tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat
    16141 taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa
    16201 ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat
    16261 catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat
    16321 ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa
    16381 caactaccat attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa
    16441 gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat
    16501 cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt
    16561 tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa
    16621 aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta
    16681 ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa
    16741 gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa
    16801 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    16861 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    16921 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    16981 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    17041 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    17101 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    17161 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    17221 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    17281 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    17341 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    17401 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    17461 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    17521 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    17581 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    17641 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    17701 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    17761 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    17821 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    17881 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    17941 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    18001 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    18061 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    18121 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    18181 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    18241 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    18301 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    18361 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    18421 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    18481 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    18541 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    18601 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    18661 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    18721 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    18781 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    18841 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    18901 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt
    18961 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    19021 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    19081 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    19141 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    19201 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    19261 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    19321 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    19381 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    19441 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    19501 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    19561 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    19621 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    19681 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    19741 agaattttcg ttttcttttg atgattcttc ttctatttcg tctggctttg gtactgacac
    19801 atctgtatct ttcttatttt ctgtctcatc aataatgtca tattctttat tatcgacgat
    19861 cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt ggaaaactca gatcgatagt
    19921 ttgtttagtg tttgatgaga tttccaacgt aattttcccg ctatttacct gataagcata
    19981 gccatctcct tcttttcttt ccacatcact taagtaattt tcgactagac caatttcttt
    20041 ttctatcttt tcagcactgg tgttattcgc atttagcttg atgttcagtg ctttttttcc
    20101 agactgatcc gaaatgactt cagctgaaat tgtttctaaa actgttggat catctgtagt
    20161 gtctgctatt gcagataatg gcacaaagtt tgataaactc aatattatca gcatgaaaat
    20221 aactttcagt ttcttccctg tggtcgtcat ttttcccctc ctttttcttt ttttctgttt
    20281 tgacgtgaat agtgaaaatt attaaaataa acaggcgtta cataatttta tttattttaa
    20341 tatgataatt attatacgaa gggcgctaaa gggaatcaca ccaattttca taccaattgg
    20401 tttcatttat gaagttgatt ccttcgatta atgagcgttt cattgaaaga aaagggttga
    20461 gggagaaaaa catatgatca ggttgttgga aggaaactat agaaagatgg aaaaaatcat
    20521 tcgaattctg tccatttctg acaagtggat gactaaaaaa gaacttgcca aacgaattgg
    20581 tagttcagaa tctacatttg taaggtatat cgaagagatc aaaatccgct ggggaacgga
    20641 tgtaacgatt caatcttcac aaaaattggg gtatcgtctt gaacatttga atgtaccagc
    20701 ttatttaaat gttctgatgg aaatgtcgca aagttctaca aacagtcgtt tattaaatga
    20761 gattattgtc aatccgggta aaacgattga gtattattgt gaaaaaattt ccatcagccg
    20821 gtcttctttt acacgtaagt tgaaacaatg caatcaaatt ttagaaagct atttattaaa
    20881 agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa agtgagctcc agttgcgtat
    20941 ctttgttacc tttttctttt taagttacta cggaagacgc acgttgccat ttccaatcga
    21001 aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt cagatgaatt tgtttcagga
    21061 agaaagccca tatgagcaat ctttctttat tatgtatttt gttgtgcatc tcatgagaga
    21121 atcccaagga actccatttg aatggaaagc tggagactct tatgtacagt tgataaatat
    21181 cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga aaattgaagt acagcagcta
    21241 tataaaagta gtgaactatt ttgtcaatac gattttttca gaattttcgg agaaagaaaa
    21301 aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac tgttcgtttt catccgctga
    21361 ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta acgcaaattt attttttctc
    21421 ccgtattttc ccatacgata cgactcattt gacgatccgt gcaacttact ttgcacagca
    21481 gatgcaacac cgccagatac gtctatatgc tggatttcgt gcagaacttc aattttattc
    21541 cactctactg aatcagaatc tgatttatta tttatctagc tatatctttt ggatgcttat
    21601 cggtaatcct gaaattcacc attatacttc tgataaaaaa atactgatta tcagcgactt
    21661 aagtcttcga catagtcaat atattgaaga atatatcagt gatattcttg ctgtgcataa
    21721 aattcactct gagacaactg ctattactga agatcagctg tcgaaataca atcttctcga
    21781 gtatgatctg atcgttacga atcaacccat tttgaatgcc catgttcctc atatattgat
    21841 tgatgatagt gtgtcattcg caaatgaaga agaactcatc cgcttattcg agttatgaat
    21901 aatcaaaaaa cagctatgta taaaccaccc tgataaaaat ttcaggacaa atacacatag
    21961 ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga tttatagcga aagtctttta
    22021 acaactcttc ttttgtccaa ttatttgcaa tgagatccgt caaagtgttc attacgaggg
    22081 tcatggaaaa gtataggaaa ctaactgctt cttccgacga ttgaatggag aaatgattga
    22141 ttcttaataa ttccagccat ttttcgttca ttggagaatc agccgatagt tctctacgtt
    22201 tggttgtctg ctgatcattt cgctgggtta acaagtataa cctttgccaa tagatacttt
    22261 cgtgatctaa gtcgcagcac cactctaaat atttttcgat ccccaaaaag aaatgttgtt
    22321 tgttttcgct aatgaatttt aagatatctt gatggactat gtgagaactg cttttgatcg
    22381 tatacaaata agcatcttcc aaatcttgaa aatatttata aaaagcaccg cgcgacatcc
    22441 ccatcttgtc aacgatttct gagactttga cttggctgat gtgctgtgag caaaatatct
    22501 ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga taaattgaaa aaagtttctt
    22561 ttggcatcca catctctcct ttatctctga ttatacgtgg atagcccttc attgtcatga
    22621 aggattcatt ccttcttttt ctcttcttct gtcaagtacc catcttccat tcgataaacg
    22681 cgatcactcc attgaatcat acggacatca tgagtaacca taatagtcgc tttttgtttc
    22741 tcatgagctt ctttaactag taatttgacg acttcatatg catgatctgt atccaaacta
    22801 gctgttggtt cgtctgctaa gatcaagctt ggctcattat ataaagcgcg agcaattgcc
    22861 actctctgtc tttctccacc agaaaggtct ttaggataac tatttttaag tgactggata
    22921 tctaaagatt gcagcaagga atcgattttt gttgaatctt ctttttttga agtaacttgg
    22981 tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa tcaaattaga gctttggaga
    23041 ataaatccta tttctttaaa gcgtaaatct gctcgttttt tttctggaag ctcagttagt
    23101 gagtgaccgt tgatcactat ttctccggaa gtcggtgttt gcaatcctcc tgaaatggta
    23161 aggaaagtac ttttacctga accagaagga ccaatgatgc tgacgaattc tccttccttt
    23221 acataaaaat caattgcttt caaagctgag acctttgtat gaccagaacc aaatgttttg
    23281 gtaacttttt tcatcgttaa tatttttttc atttttatta acctcctatt gctgtaattg
    23341 ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc tagcattgcg acaatgatca
    23401 aaatcccact atatagcaac cattgtgacc aaaaaacagc aaaaggcatc gcgcttggta
    23461 aaatcagact agtcaaataa gcaaacaaaa gagcaagcag aacgcctacc accccaacaa
    23521 taaaggactg tgctactaaa gatttagcga tgaagaaatt tttgatccct tgtgctttca
    23581 ttactccaaa aatagcagtc ttctgaagtg tcatcacgta catgaaaata ccgaccacgg
    23641 cagcaaccac taagaacaaa aagtatatca tcgcatttaa ggtcatattt tgggcagaat
    23701 agccaggaat actttcaatg aatgtcccaa tagttaataa ctgtagaccc ttcgatgctt
    23761 tttctttcga aattttagct gaatccttta ttacgatccc attgatcggc tgttcttctt
    23821 cagaagcaaa cggctgttgt ccatacttca aagcagtcca cgtatctagg gacgcataga
    23881 tgacgggaga aactgtgtaa taagtttcag gaaagatacc gacaatcgtc aaaggatcgt
    23941 cgtaactgcc aattttgatc gtatctccaa ttttatagcc tgaatcagcc agattttgag
    24001 agatgatgat ttcattcttt gttttaaatt catgcccttt cgtgagtttt ggtaataaaa
    24061 atgcttcttt cgttgtgccg aaaactgtaa cattttcttt gttttttcct ttgattgctc
    24121 cgctgtacaa tccgattggt gctttctctc ctccttcaat gtatttcaga tctcctcttg
    24181 ttaattgtga agctgcaaat acttgatttg catcctccga caaaacaatc tcttgtgcgt
    24241 cccaatcatc gattgctttt ttaaactctt ctgaaagtcc attcgctagt cctgacaaca
    24301 tgaatacgac atacgcaatc aagaacataa taccgacgat caagccatat cgtaatttag
    24361 aatagcgaat ttctttgata gctagaaaca tttgtttttc ctccctatga tagattgata
    24421 gtgacaaaat gtcacttgtt gaatagtgta actctaaagt gacaatttgt cacttgtgtt
    24481 gtttttttct tatcaataaa ttgtctaaga catctttcga taaaacctta acatgagtca
    24541 taaaaaattt atttaagcct tatttatgct ttccatgata aactaaagtg accaaatcaa
    24601 tgaggtggga aaatgattaa actattagta gtagaagacg ataaagtatt atccaataat
    24661 ataaaagaaa tactcgctgc aatcggcgag ataacacaag tttatgatgg tagtgaagga
    24721 ctgttcgagt tggaaagtgg agtttatgat cttgcaatct tggatttgat gatgccggaa
    24781 atgaatggat atgaagtgtt agcaaaaata cggaaagaaa aaatacagac tccagtattg
    24841 attttgactg caaaagatag tttggaagat aagattgaag gatttgaacg aggtgcagat
    24901 gattatctaa caaaaccttt ctatcgagaa gaactgatca tgcgcgtgaa agcattattg
    24961 aaacgctcgc taggtctctt tgatgaaaat gttctgaagt ataaggatat cagttgtgat
    25021 ttaaaaagca atgaagtctt gtataaagaa caagttttgc ccatacaagg aaaagagttt
    25081 gaattgctcg tttatttttt acaaaataag ggaatcattt tgacaaaaga gcagatattt
    25141 gaccggatct ggggatttga ttctgatacg acgattacag ttattgaagt ttatatgagc
    25201 aacttacgaa aacatctgcg aagtactgga ttggataaag aaatcaaaac cttacgtaat
    25261 gtcggttata tactgcaaag ggtgtgatgc aatgaatgga gatattacac gaaagcaacg
    25321 tagtcgcttt tttgcgacaa atgtagcagc atttgctttt atcttttttc ttttgggatt
    25381 cattacttta cagatattaa accaatctgc atacagagaa acagatctgt cattagagaa
    25441 aataaatttt gattctcgaa cagtacaatt agagatacaa agatatcagc aaggcgatcc
    25501 ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac atggaacagc cattaatgga
    25561 accatcaaat gaccgtttta acactcaagt gatcttgtgg tctaaaaatg gtgagatatt
    25621 gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa gaattaaaaa tggatacgaa
    25681 aaatctgaat accatccaaa atattgaatt aaatgaagac agtggctcat ctgccctctc
    25741 tttccgttcg attacgaaaa aagcggagac gaatcaagat ggcgttgcct acatccaaat
    25801 tttagaaaat acaaatcaaa tcaagaattc attagcgact tttcgcacga ttatgatttt
    25861 gtgtatgatt gttttttgga ttctctctat tggtatcagt tattacttgt ctagtctaag
    25921 catgcgccct attattcttt cttggagaag acaaaaagaa tttgttgaga atgcttctca
    25981 tgaattgcgc acaccgttaa cgattattca aaatagtttg gaacatcttt ttaccaagcc
    26041 taatcatact attattgaag aatccgaaag catcgctcaa gcattgtcag agacgaggcg
    26101 cctcactgga ttgacttcgg atttgctgac aatcgctcgg aatgattctg atcagcagct
    26161 gctttcaaag caaatgatca atacacaaga atatatcgaa caacttgtta aaccatttca
    26221 agagatggcc atcatagatg gaaaagagtt tattctccat aacgatgcaa cattagaagt
    26281 accaattgat gaaaaaaaaa tccatcaagt tttggttatt ttgctggata atgcattaaa
    26341 atatacgaat ccagaagata agatcatttt ggattcatct gttaaccata aagaatggct
    26401 gattcacgta aagaatactg gcccttctat cagtgaagaa gatcaagcaa ggatttttga
    26461 tcgattttat cgggaagatc aatctcgggc gaaagagact ggcggatacg ggcttggctt
    26521 ggcaattgca aaacaggtaa tcgaacagca tcatggacag atttctgtat ccaattatct
    26581 tccacaagga gtcgatttta aagtccaatt gccgttgaag cctaaaaaat aatgcttatc
    26641 cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt ttttgttgcg aataaacggt
    26701 gttccagaag tagcttcttc ggaaataagc cgaaatttct ttaagctgac cacttctgtc
    26761 acaacctctt tagcttacgc ttacgatttt ttattgcggt cttcaatgtg tacatcaccg
    26821 cttgtgtcaa tatccaattc ttctcggcta acatcttctg tcactttttc aacatcttca
    26881 tgttcttctt tatgaatccc aacttcttca cgaataactg tatgtttgct tacatctact
    26941 tgctcttctt tgattgggat agtaatcgtg tcatcttcca atttttcgtc tgttccctgt
    27001 gaagattgat ctgttactgg tttgcgttcg atgacgattt cttcacgttt gaccggtact
    27061 tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt cgacttctcc agttgtcaca
    27121 tcatgtgtat ttacatctaa tttttcttct tttagttgaa ttgtatcatc atctagattt
    27181 ggtttgcgat cgatgtcttc agtcttgctt gtacccattg tatcagtctc gccaggtacg
    27241 tccatcggat cagttcctgg tgtcatattc aatggatccg ttgtattttg aaagcctgtc
    27301 gtgccaccta cgtccattgg atctgttcca gtgccagttt ctaatccagt ccccattgtg
    27361 cttgctgggc taacagtatc ttgtgtatcc attgtatctt ctgttgtttc tggctgacga
    27421 tagcctttta ctgtgatgac aaattttcca tcagaaatat ctcctctata ttgatataaa
    27481 ggatcatttt ctggtgtagc cgtctcagaa tcataggtat caaaagaaaa agtatctttt
    27541 attttttccc ataaagaacg gtcttcttca ccatccatcc gattttcatc tgtttcaaca
    27601 tctataccag acaatccgtc aaatcctagt ctagatgctg cttcatcagt agtataaata
    27661 acgatatcat ctcgttcata cccttcgtta cgcaaccgtt caattactga taatgcttct
    27721 tccctcgtac tatagcttcc gatgaccgtt aaatttttct cttccatgta aattcctcct
    27781 agcaaataga tttatgattg gtatattgtt acttataaat ttattctata ggagacgatt
    27841 gttttctaca aatatttaga ttggaatttg attaaaacaa tttaattgaa atttgaaaac
    27901 atttatgtta atgaaagtca tattctaata aaatgtcatt tgtttctata tgaattttta
    27961 tgaaaaattt catataattt ccactcgttg tgatggttta atttatccaa tccatcaata
    28021 agcaaacaac ttatataaaa aaggattaat tctcaatata attgctccta ccatagaata
    28081 aatcttcatg taaagtaatg aagtaccaac tatttctaat acttctttaa gaattatccc
    28141 aacaatatcg ttagacaaac cattattgaa taaatggaga gccaatcgat tctgaaaaaa
    28201 tgttttctaa tttttcggaa ataaaagaaa gagcctcctg ccattagaac gaaccaaatc
    28261 catttcattg ctcatgaagt aaccacatag ctaaaaagag gataaatcaa ttaaaagatc
    28321 aaaagaatga ctgcactaat aaaataacta aaaatgtatc ttcttacttc atatctttta
    28381 gctagtactt agttaaaaat ttgcttccta agatcaagac aaactgtcta tcttcatttg
    28441 atgaaatgcg gtaaaatata gaaaatgaac actattggag ggaatttgta atggacagca
    28501 aaactagaaa acaactgcaa gaaaagttaa agcaacgtac acacttgctc ccttccgtct
    28561 ttcaacagtt tttagaagaa aatcgttctt ccctttcatt aggcagtcgc tatgaatacg
    28621 caaaagattt tcatctgttt actgattatt tgagaaaagt attttctgaa gaagtggatg
    28681 atcagctgat tatccattta gaaaaacaag attatcagaa tttcttagct aaaatctatc
    28741 gacatactcg ttcgttccaa actacagcga accaagaaac ggagcaattg tttgaaaata
    28801 gctattacgg gatttcaagg aagcaatatt cattaaatca ttttttacga tttttgtatc
    28861 aaaaaggact gattgaagaa gagatttctc ttttgggtac aagtttaccg gaaacaacca
    28921 aagcagctcc gcgatatatc gatgcagaac ttttcaaaga ttttgagcat ttgtttatcc
    28981 cgattgaagg ctttcaaaca agcagagaag ctagctttga agaaaagaat agaccgagga
    29041 accttgcaat tactgccatt cttctgtatt gcggattaaa gatccatgaa gtagtagaac
    29101 ttaaacgtaa agatgtatct ttgaccaagc agattcttaa tctgaataga aaagaaaaca
    29161 gactgaataa agtaccaata ccaaaagaag cgatgccatt tctggagaac tattttaagt
    29221 taacagcaca aaatacggac gaaaattctt ttgtttttcg ttcctcacat gatcagcaaa
    29281 tttctcctcg gactattcgc caaattttga gcaaaattac agtatataaa aacgtatcgc
    29341 cagaactatg cagaaaaaca tatcgttatt atgcagaact atatttagat gatgctgacg
    29401 cggtaaatta tttatgtggt aatcgatacc tctcatccca ttcttttcaa gatatctggg
    29461 aaaagatggt tgatttttct tatcacgaat tagcggcatt cgttcgggtt tcttccatat
    29521 aaaacaaatg tatgatcttt ttttcacaat aaactatgtt attatataaa ggattatgtt
    29581 acgattatct tgaatttgta tattacttaa caaaagataa tggagtgtga atgatggaga
    29641 ccataacgta ttcccctttg aacttattta cgaattttca agaagctgca gctaactttt
    29701 ccgagacccc gattatcttt gatgagccac tttcagcttt cccagaatta ggacttgaaa
    29761 caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc ttatcaattg gctcatcttg
    29821 gagtgaaatc cggagacaaa gttattattt ataagagttc cgcttttgac acataccttc
    29881 tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat gacttcttat catctaccaa
    29941 caacaacaat ggaagtattt atcgatcgtt tagaagaccc tttcatttta tttgatgatg
    30001 agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa atcaaaacag attcctaacc
    30061 cgtggtgcta gttaattttt tcctagagga aaatgtctaa acatgttggt aaatcaacaa
    30121 aaaaacgcat atagtagtct caaaactccc aagaaatgag gactacatat gcgttcatca
    30181 aaatatactg aacattatac agatactttt ataacattca aggaaattat gagaactgtt
    30241 tctaacatat accacaattg tgtaccagat aagattaaaa accgaagaaa tactgatcaa
    30301 ctgaag
//
LOCUS       DAYYNQ010000024        30285 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000024 DAYYNQ010000000
VERSION     DAYYNQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.024"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            933..1247
                     /locus_tag="ACHBJ1_000986"
     CDS             933..1247
                     /locus_tag="ACHBJ1_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429950.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(1300..2073)
                     /locus_tag="ACHBJ1_000987"
     CDS             complement(1300..2073)
                     /locus_tag="ACHBJ1_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429951.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            2178..2423
                     /locus_tag="ACHBJ1_000988"
     CDS             2178..2423
                     /locus_tag="ACHBJ1_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9429952.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            2579..3430
                     /locus_tag="ACHBJ1_000989"
     CDS             2579..3430
                     /locus_tag="ACHBJ1_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM9429953.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            3463..4140
                     /locus_tag="ACHBJ1_000990"
     CDS             3463..4140
                     /locus_tag="ACHBJ1_000990"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM9429954.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            4628..5593
                     /locus_tag="ACHBJ1_000991"
     CDS             4628..5593
                     /locus_tag="ACHBJ1_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM9429955.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            5615..5827
                     /locus_tag="ACHBJ1_000992"
     CDS             5615..5827
                     /locus_tag="ACHBJ1_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9429956.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            5902..6378
                     /locus_tag="ACHBJ1_000993"
     CDS             5902..6378
                     /locus_tag="ACHBJ1_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM9429957.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            6375..8183
                     /locus_tag="ACHBJ1_000994"
     CDS             6375..8183
                     /locus_tag="ACHBJ1_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM9429958.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            8260..8475
                     /locus_tag="ACHBJ1_000995"
     CDS             8260..8475
                     /locus_tag="ACHBJ1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM9429959.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            8567..9430
                     /locus_tag="ACHBJ1_000996"
     CDS             8567..9430
                     /locus_tag="ACHBJ1_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM9429960.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            9467..10003
                     /locus_tag="ACHBJ1_000997"
     CDS             9467..10003
                     /locus_tag="ACHBJ1_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM9429961.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            10017..10439
                     /locus_tag="ACHBJ1_000998"
     CDS             10017..10439
                     /locus_tag="ACHBJ1_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM9429962.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            10369..12768
                     /locus_tag="ACHBJ1_000999"
     CDS             10369..12768
                     /locus_tag="ACHBJ1_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM9429963.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            12800..14791
                     /locus_tag="ACHBJ1_001000"
     CDS             12800..14791
                     /locus_tag="ACHBJ1_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM9429964.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            14814..15065
                     /locus_tag="ACHBJ1_001001"
     CDS             14814..15065
                     /locus_tag="ACHBJ1_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM9429965.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            15055..16284
                     /locus_tag="ACHBJ1_001002"
     CDS             15055..16284
                     /locus_tag="ACHBJ1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM9429966.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            16281..18362
                     /locus_tag="ACHBJ1_001003"
     CDS             16281..18362
                     /locus_tag="ACHBJ1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM9429967.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            18511..22431
                     /locus_tag="ACHBJ1_001004"
     CDS             18511..22431
                     /locus_tag="ACHBJ1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM9429968.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            22432..23376
                     /locus_tag="ACHBJ1_001005"
     CDS             22432..23376
                     /locus_tag="ACHBJ1_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429969.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            23380..23583
                     /locus_tag="ACHBJ1_001006"
     CDS             23380..23583
                     /locus_tag="ACHBJ1_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM9429970.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(23883..24119)
                     /locus_tag="ACHBJ1_001007"
     CDS             complement(23883..24119)
                     /locus_tag="ACHBJ1_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9429971.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(24302..24673)
                     /locus_tag="ACHBJ1_001008"
     CDS             complement(24302..24673)
                     /locus_tag="ACHBJ1_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM9429972.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(24670..25998)
                     /locus_tag="ACHBJ1_001009"
     CDS             complement(24670..25998)
                     /locus_tag="ACHBJ1_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9429973.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(25959..26288)
                     /gene="mobC"
                     /locus_tag="ACHBJ1_001010"
     CDS             complement(25959..26288)
                     /gene="mobC"
                     /locus_tag="ACHBJ1_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM9429974.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(26546..26916)
                     /locus_tag="ACHBJ1_001011"
                     /pseudo
     CDS             complement(26546..26916)
                     /locus_tag="ACHBJ1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            27061..27246
                     /locus_tag="ACHBJ1_001012"
     CDS             27061..27246
                     /locus_tag="ACHBJ1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9429975.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            27404..27571
                     /locus_tag="ACHBJ1_001013"
     CDS             27404..27571
                     /locus_tag="ACHBJ1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9429976.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            28189..28848
                     /gene="vanR-B"
                     /locus_tag="ACHBJ1_001014"
     CDS             28189..28848
                     /gene="vanR-B"
                     /locus_tag="ACHBJ1_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9429977.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            28851..30212
                     /gene="vanS-B"
                     /locus_tag="ACHBJ1_001015"
     CDS             28851..30212
                     /gene="vanS-B"
                     /locus_tag="ACHBJ1_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9429978.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         8007 a         7511 c         7873 g         6894 t
ORIGIN      
        1 tctattatca ctattaaaga gaaagttgga gcggaatata aagaaaagag aaaagtaatt
       61 aaacgcaaaa aaatgtagaa cttaaaaaat ataaagagga acaaccgtta aaattaggaa
      121 tgatgttgtg taaacattat aattgctatg atataaatga agtgttaaag aaagcaaatt
      181 taaaccaatt ttgtgaacag aaaagcctta gtcactatga ccaaaactac ggacgaaact
      241 atgaccaaaa gtaggtacta aattatgacc gaaaataggt ccaaaatgga tttttttatg
      301 atatcaaaaa ttatgaaatg attttgaaag aaaaccagtt ggcacagcca acttagccac
      361 cttcctgtag gaaggtgcta tcagttttcc cttatgctct tttctttctt ttttgtttca
      421 tatgtcaaat gccattttga aatgtaccat cttaagaaat ttatccttaa aaacaattgc
      481 gttattggat aaaaagatgc tacattatct taaaaaagga atcaaatatg atgaatttag
      541 tggctagcat atcgtgtgat taagatactt tatagtgtaa gttgaaggag aaaaaatatt
      601 taaaatatac ttcccatttc agtgggaagt aaaattaatc ttgttgtttc tactagtaat
      661 gtatcttttt ggaaactatg taacgatgta aacaagattt ttttcagaag aaaaaacgat
      721 aaaagttgtg ataaacgatt taaaaaatag gagaaaatat atcaagcgca tggtatgaaa
      781 cgcacattta aaaagattcg actaactaaa aaaataagtt tacaaagcat tgttgtgtat
      841 tacaagtgat ctatacactg aaaaatgata gagaaaatct gatacgtccg caacaatgga
      901 caattgaaaa tgaaagtgag gaaattggaa gaatggctga tgaaaataaa gttgttccgc
      961 ttgaaacact agtgataaat aaggaagagt ttgagcaact ttttcttgat ttttttgttc
     1021 aatatgacag gatttatgat tttagttcgt cactagattt atttgaatta gaggcagaaa
     1081 taatagaaaa cagtcgagag gagaatggta aaatcatcat ttcagtagaa aaattcaatg
     1141 aattattctc agaaacattt gaaatagata cagattatta tcaaccacaa gaagttttcc
     1201 aatcatttag aaatcagatt attcgctcag gtaagaccgt aaactgaaaa acaataaaag
     1261 aaacgttgaa agaataatta ttccttcaac gtttcttttt tagaatgttt taatgaccaa
     1321 agcagtttca ttgtcaggct taggtaattc atgaaatccc gtatttatag tgctcaattg
     1381 atcaactaac catgttttac tttttgaatt gtagataaat ctaaaattaa tatcgttatt
     1441 atcgtgttga aggggcgtct ccaaataatc tgctgtatat tcatagtcaa cgtaacctgc
     1501 taatacaaaa gagagtatcc ctttatttaa tgttagattt aatggataat catacgactt
     1561 tatttttttc aacttcatat tgtacatatc ttcagccata aaagttgagt tcgtgatttc
     1621 atcttgggtc tcattaaaaa agttatccga ataatcgctt atttttgaaa agtctttatt
     1681 ggcaccagcc tcaaaaaatt gttcaacttt ttcagtataa agttgtgcta acgctccttt
     1741 ttcaatatta actttaatat tttcatgtct atcaatttgt gtgctgttca gtaagggaag
     1801 atcagctttt agttttatat cgtcttgatt tctttcaatt tcaattggca aaatcttttc
     1861 agtagattca gtttctgata aaggaacatc tagtttttta tcatgataat aagcttcaaa
     1921 tccatctgga accggaaaaa aatgtattgg gtaattcttt tctgaggttg ttgtactagt
     1981 ttccgtactt gtagaacttg tgtcagaagt ggtacacccg cttatagttc cgatgagtaa
     2041 agcgcaagtt agaatgattg caaatttctt caaaaaaatt cctccttaaa attgaatatg
     2101 gactaatact agtcccattg tagcataccg aatctttaag attaataaga aaaaggacta
     2161 attttagtcc aaaaggtgtg ctattcttga aagaggaaaa aattattgat aaagaaaatg
     2221 taatcggtag caatattaga cgagtccgat tagaaaaagg aattggtcaa accgagctga
     2281 ttagagacct acagcttcgt aaaattgcta taactaggga aacgttggtt aagattgaag
     2341 gtggccgcca acacattaag ttggatcaat tgaaagccat caaagatatt ctacaggtgt
     2401 cttatgaaga tttgcttcaa tgactacttg tagtcatttc gtttgaaatt attatgttac
     2461 acccttatgt gtcacgaaga aatactggta taataatgaa tgggatatgt aacgataggg
     2521 tatactgtta tgttgcctaa taaattgtgg atcaaaatac aagaatggag ggatatcctt
     2581 gtataaggca tatgaattgg attttacaaa tgctgactta aatgcattct cctcaagctc
     2641 gcaatcatat acacatgtag taaatcaaat agatcacaac caaaaatcta ttaacaagag
     2701 aatagaaaat ctatttgaaa gtgaaaatga tttagttgat tattattcta aggacagcaa
     2761 aattctagat gcagacgaaa ttattaaaga ttggtttcca actataaaag cagatatttt
     2821 tatatctcac tctcatgctg atgaaaaatt agctatacgt tttgcatcat ggctatttga
     2881 aaattttggt cttactgcat ttattgattc ttcggtttgg ggatacagtt ctgatttatt
     2941 aaaaaaaata gaccaaaaat attgctataa ggaacaaaca aaaacatatg attatgataa
     3001 aagaaatgta acaactagtc atgttcatat gatgctttct acagctttaa ataatatgat
     3061 tgatagtaca gaatgtctgt tttttcttaa tacacctaat tcaattagtt tgagcaatga
     3121 aatcactaat gaacagaaat ttacgtattc gccatggcta tatagcgaac tgacgacagc
     3181 ttcaatagtg gaaaaaaaga atccacgatt ggaaagtaat cctcaaatga gcaccgagga
     3241 tgttagaagc attataaagc actattcttc gcaagaaaat ctgcaaataa aatattcttt
     3301 aaatttaagt gaattaactt atttacgaaa agctgatttg aaaaaatggg ctttagatgc
     3361 tgtggaatgc actaaaggaa gtaatacatt agatatttta tataaaaaca ccattttcac
     3421 taatctctaa tcaatttact gaatcgaaag gaaccgggta gaatggataa gaaagtttta
     3481 ataaagaggt tctctgaagc tattgaaaaa ggaaatgcag caatatttgc aggtgcagga
     3541 ttatcgatgt cacaaggata tgtaagttgg ccggaactac ttaatgatcc tgcaacagaa
     3601 atcgggttgg attctaaaaa agaaacagac ttagttactc tggctcaata ttataaaaat
     3661 gaaaatggtg gatcaagagg aattttgaat cagatactta tggataattt tggtgaagaa
     3721 ttagaaatat ctgaaaatca tcgaatacta gcaagtttac ctattgaaac ttattggaca
     3781 actaattatg atcatctaat agaaaaatca atccgagaag cttataaaaa ccctcaggtt
     3841 aagaaaaatt atactcagtt agcaacaact aatccaaatg tggatacaat tgtttataaa
     3901 atgcatggtg atatagatga tgtcagttcg acagtaataa caagagatga ttatgaaaaa
     3961 tatgatgatt cgtacgcgtt atttaaagaa actttaaaag gagatttgtt aacaaaaact
     4021 tttctctttt taggatttag ttttacagac cctaatttag aaagaatttt atcagatata
     4081 agatgggttt tgagagaaaa ccaaagacct cattattgta taatgagaaa aatattatag
     4141 aaaaaatttt aggttatcgc tgaaagcccc ggaaatacgg gcatttcgca ggatatgaga
     4201 actcaattta ctaccaattt actactttcg gattgagccg catatatgct gaaccatact
     4261 gctaataccc gccaccccaa atacggcaca tcataaattg aatacgacac cgtaaacgcg
     4321 gcgttcctct ttcccgtcat gggagaacag gacgccgctt ttttatgccc aaatagagaa
     4381 aggaggcgac gccatgctat ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca
     4441 ggaaaagccc ggtttcgatc actacgacgg cggcggggcg gacgcgccgg aggattgcgg
     4501 cacctgccga ttttaccgcc cggattggaa atatcaattc tgccagtttg cagagtgtcc
     4561 ctatcagccg ggcaagctga ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa
     4621 caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca
     4681 ccacctgcgg aataagaacc tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc
     4741 cttgccggag gattgggact ataccctaaa aggactgtcc tatatcaacc gggaaagcat
     4801 cgacgcgatc aggacagcgg tatgggagct tgaaaaagct ggatatatcc ggcgtgagca
     4861 gggccgtgac gcaaaaggca aaatggccga tatggtctat accatctacg aacagccggt
     4921 attggaaaac ccggtattgg aaaatccaac atcggataac ccggtgttgg aaaatccgac
     4981 atcggataat ccggcgtcgg aaaatccaac gcaattaaat aaagaagtac aaagaactga
     5041 cttatcaaaa aaagaaaaaa taattacaga tggacaaagt acccattcca ttcctatccc
     5101 atccccgacc cctgccccta tggagggcga ggcagcggct acgccgccgg aacggaaaag
     5161 gacaggaaag gatgaggctg tacggattta tcgggaaatc attttggaga acatcgagta
     5221 tgcctatctc attcaggaca ggagcattga ccgtgaacag cttgacgaaa tcgtagacct
     5281 gatgctggaa accgtctgca ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc
     5341 tgccgagctg gtgaaatcca agtacatgaa gctgaccggc gaacatatcc gctttgtcct
     5401 tgactgtctg cgggagaaca caacgaaagt ccgcaatatc aaacaatacc tgcgggctat
     5461 gctgttcaac gcccccagca ccatttccaa ctactacacg gcccttgtcg cgcacgacat
     5521 ggcacagccc gattggggaa agcctaaatc cggcctgccg gactattcct gcggcgaggg
     5581 cgaaagcctg taacgagaaa aggaggacga ccctatgaag cagggcgcat tgatttttga
     5641 cgagcgaaca gaccgttacg acatccgctt tgatctggcc gactattacg gcggcctgca
     5701 ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat
     5761 ggccgaaaac tggtatcttg tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg
     5821 gatttgacct atatctacct gcggcgtctg gtggttccgc cttagacgcc gcttttgact
     5881 ttctgacaag gagggatgcg attgcaggaa gaagttactc aaaagaccat tgccctgtcc
     5941 atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa
     6001 caccgggcca agggcaaaaa cacgctgcac cacggccagc agagcctaaa gcagcttaaa
     6061 aagcatgggg ctgatctctc caacattgag atcaccgagg ccaacattgg agcgttcaag
     6121 ccatgcgcca agaaatacgg cgtggatttt accttacgaa aagataagac cacccagccg
     6181 ccccactacg ttgtgatttt caaggccaag gatgcagata accttgatct ggcgtttcgg
     6241 gagtttaccg ccaagacgct ttccaaggag cagcggccct ctatccgcaa ggttttgtcc
     6301 gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa
     6361 agggggctgg aactgtgaag cctgacgtaa agaaattgct gatcctgaac ctcccgtatc
     6421 tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg
     6481 acctgtcggg gaagctcctg cattttgcgg atggttttgc ggcggctttt gccaaccctc
     6541 tgccaagcct gcacctgttt gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc
     6601 tggtggtgta ctgcaagagc aagaacgtga agaaataccg caaaggcatg gagtatggca
     6661 gcgcccgctg ggggacggcc aaagacatag cgccttacat cgaccccaag tttgaaaata
     6721 atatcctgct cacccaaacg gaacggctta ccatgaccgg caggcccaaa gaccccaaga
     6781 cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt
     6841 tcgtgaaacc caacctgatg caatgctttc cgaccacgga ctatccgact tcgtttgtgg
     6901 tgacagatcc gaaaggcact ttggttctgg aaaccggcag gatgttccag caggcgggct
     6961 accgcattaa aatcctgaat accattaact tttccaagtc catgaagtac aacccctttg
     7021 tctacatcca ttcggaaaag gacgtgctaa agctggtaaa taccctgatt tccaacacca
     7081 agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt
     7141 cggccctgat cggctatatc tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc
     7201 tgctggaaat gataaacgcc agtgaagccc gcgaggacga cccggagttt caaagccccg
     7261 tcgatctcat gtttgagcgt ttggagcaga aagaccccga ccattttgcg gtgcgccagt
     7321 acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc catcctgatt tcatgcggtg
     7381 ccagacttgc gccctttgac atccgggagg tacgcgagct gatggaggac gacgaaatgg
     7441 agctggacac catcggggac gaaaagacgg tgctgtatct cattatgagc gatacggaca
     7501 cgacctttaa tttcattctg gctatgctcc aaagccagct aatcaacctg ctttgtgacc
     7561 gggctgatga tgtgtacggc gggcggctgc ctgtccatgt acgcctgatt ttggatgaat
     7621 ttgccaacat tggccaaata cccaatttcg ataaactcat tgccaccatt cgaagccgtg
     7681 aaatatcggc ctctattatt ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg
     7741 ccgccgagat catttcggat aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa
     7801 atgccaagga gatttccgat gcgctgggga aagaaacgat tgacagtttc aacaccagcg
     7861 agaaccgggg aagccaaacg tcccacggat tgaactatca aaaattagga aaggagttga
     7921 tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg tatcttgcag ctacggggtg
     7981 tgcgcccgtt tttatcggag aaattcgata ttaccaagca tccccggtac aaataccttg
     8041 ccgacgccga caagaagaat acctttgacg tggacagatt tttgactatc acgcgccgga
     8101 aacggcagca agtggtaacg caggatgaag tattcgacct gtatgaaatc gacctgtcgg
     8161 acgaggacgc cgccgctgaa taaacagcgg cttttttcat tccaacaaca aaatcaaaaa
     8221 atcattatga gccggaaacc ggcagaaaga ggtaaattta tggcattttt taattccgca
     8281 gttactactt tgcagactat cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc
     8341 atcaatctgt tggagggata cgggcaggac aatccggggg caaaatcgca gggtgtcaag
     8401 cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca ctctggttcc cctgctgtcc
     8461 gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc
     8521 gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat
     8581 cattgaagaa tggataaaag gcatcctcat ggacggtatc acgggcaacc tgtccgggct
     8641 gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc gacgtgggac gaaccccgca
     8701 ggcatggaac agcggtattt tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc
     8761 catagccggg gccatactcg cccttgtcat gtgctatgag cttatccaga tgataacgga
     8821 aaagaacaat atgcacgact ttgacagctc catgttcttc cgctggatat tcaaatccgc
     8881 gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc atgggcgtat tcgacgccac
     8941 ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg cagacatcca ttgattttaa
     9001 caccctgata ccagaccttg aaagccaatt ggaggctatg agcgtgggcg ctcttttggg
     9061 gctgtggttc caaacccttg ttgtgggcct caccatgaat atcctttcca tctgcatttt
     9121 ccttgtgacc tatggccgca tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc
     9181 ccttgccaca atgggcaacg cggaatggcg cggcatgggc caagggtatc tgaaatccct
     9241 gctggcgctg ggcttccaag cctttttaat catggttgtg gtgggtatct acgcggtgct
     9301 gatacagaat atcggttccg cttccgacat atccggcgca atctggggct gtatgggcta
     9361 tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg
     9421 ggcgcattga tcccaaaatc caagcataca caggaggaac ttgtttattt caacgtacaa
     9481 catcatttac gctgacccgc cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga
     9541 aaatcactat tccaccatga gcattgacga gctgtgcgcc ctgccggtgg aacggctggc
     9601 gtccaaagac tgcctgctgt ttttgtgggc cacattcccc atgctgccgg aagctctgcg
     9661 gttaatcaaa gcgtggggct tttcattcaa gaccgtggct tttgtatggc tgaaacagaa
     9721 ccgtaaaagc cttacatggt tttacgggct gggccgctgg acaaggggaa acgcggaaat
     9781 ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat
     9841 tatcagcccg attgaacaac acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga
     9901 gctggcgggc gatttgcccc gcgtggagct gtttgccaga cagaaaaccc ccggctggga
     9961 cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg
    10021 cttatgtaac cgtccccaag gacttaaccc atgtgaaaag caaggttgtg tttgggctta
    10081 ccaaacggca actgatctgt ttttccgggg cgctggtgat cggcgtcccc ttatattttc
    10141 tcacccgtga ctatcttacc aacagcgcgg cggccctgct catggtattt gccatgctgc
    10201 cgggcttttt gcttgccatg tacgagcgtc acggccagcc ccttgaagtg gtgattatgc
    10261 agatcatcca gtgctgcttc atccagccaa aggagcgtcc ctatcagacc gacaacacct
    10321 acaccgccct tgtgcggcaa taccagatgg aacaggaggt aaaggccatt gtccaaaaag
    10381 gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac
    10441 gcggtaatcc gtaaatataa gggcgacggc aggccccaca cggcgcagca gagcatcccc
    10501 tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc
    10561 attgagtttg cggacatcag ctatcagctt gcccagccgg acacgcagac ggctatcttt
    10621 gaaaaactgt gcgacctgta taactatgtg gacgcctcca ttcatatcca gttttctttc
    10681 ctcaatcgga aagttgaccc ggtgcagtac gccaagagct ttgagatcgc gccgcagggg
    10741 gacgacttcg acgacatccg ggccgagtac accggcattc tgcaaaagca gcttgcaaac
    10801 ggcaacaacg gcatggtaaa gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg
    10861 aaagcggcgc gagccagatt gaagcgcatc ggctttgacc tgttaggcta tttcaagagc
    10921 atgggggccg tagcccatgt gatggacggc tgggaacggc tgaacctgct gcatggcgtg
    10981 taccacccgg acggcgagat attcaatttt gactggaagt ggctggcccc gtccggcctc
    11041 tccaccaagg attttattgc cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc
    11101 atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac
    11161 gatatgctgg ccgacttcct caatacggaa agcggtgttt tagtcaatct ccatgtgcag
    11221 gccattgaac agaccaaggc gatcaagacc atcaagcgca aaattaccga ccttgacgct
    11281 atgaagattg cggagcagaa aaaggccgtc cgcagcggtt acgatatgga cattcttccc
    11341 agcgaccttg ccacctacgg cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc
    11401 aatgaacggc tgttccagtt gacgttcctt gtcctcaacg tggccgacac caagcaaaag
    11461 ctgaataacg acgtgttcca agccgcaggc gtggcccaaa agcataactg tccccttgtc
    11521 cggctggact accagcagga acagggcctt gcaagcagcc tgccgctggg cgtcaatcag
    11581 atcaagatac agcgcagcct tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg
    11641 caggagcttt ttcagggcgg cgcggccatg tattacggca tcaacgccaa gtcccgcaac
    11701 atgattatgc tggacaggaa gcaggcccgt tgccccaatg ccttgaagct cggcaccccc
    11761 ggcagcggca aatccatgag ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct
    11821 gacgatattt ttatttcaga cccggaggcc gaatattacc cccttgtcaa acggctccac
    11881 ggacaggtaa tccggctgtc gcctaccagc aaggactttg tgaacccgct ggacatcaac
    11941 ctgaattatt cggaggacga taacccgctg gccctgaaat ccgactttgt gctgtcgttc
    12001 tgtgagcttg tcatgggcgg caagaacggg ctggaagcca ttgaaaagac cgtgatagac
    12061 cgggctgtgc gcgtgatcta ccggccctat ctggcagacc cccgcccgga gaatatgccg
    12121 atcctgtccg acctccacaa agcgttgtta gatcagcatg tcccggaggc tgaccgggtg
    12181 gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg tatttaacca ccgtaccaac
    12241 gtggacatcg gcaaccggct tgtgtccttt gacattaagg agctgggcaa gcagctcaaa
    12301 aagctgggga tgctcattgt ccaagaccag atatgggggc gcgtcacggc caaccgaagc
    12361 caaggaaagg ccacatggta ttttgccgac gagttccatt tgcttttgaa agaggaacag
    12421 acggcggcct attctgcgga gatttggaag cggtttagga aatggggcgg cattccaaca
    12481 ggtgccaccc agaacgtcaa agacctgctt tcctccccgg aaattgaaaa tatccttgaa
    12541 aacagcgact tcattacgct gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa
    12601 cgcttgaatt tgtccaccga acagcagaag tacatcgaca attccgaacc cggcgagggc
    12661 cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc ccatcccgca caacacccag
    12721 ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc
    12781 acaaaggagg tgaaccacca tgaaagagct gaaagccaag gcgaaagtga cgcaggccat
    12841 gacccgcgac gggctgacgc tggaaaatca ggccaccggc gaggtggaga acatttccag
    12901 ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct
    12961 ggaacgggcc gacgccatcc atgacggcca taaggccaag aaagccgcga agcaggccgg
    13021 ggctgtggcc gccgaggggg acgctgccct gcaccggcca tccgcccggc tccaactgtc
    13081 ggaggaagaa cgggccgacc cggatttaca gccctatatc cgaaaatcgg acaaacgggc
    13141 cgataagctg gataaggccc gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac
    13201 cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa
    13261 ggggcctccc tccctgaaac ccaatccggc aggccgtcct gtttccgagg tttttctatt
    13321 cggccacggg aaaatccatg aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa
    13381 gggggaggaa cttgccgagc ggcaggccgg aaaagccatc cgcagcacca tccggcacca
    13441 caggctgaaa ccctactggg acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc
    13501 ggagtatttc taccagaaat ctttgcggga taacccggag atcgcacagg cggccagcaa
    13561 ccccatttcc cggttctggc agaaacagca gatcaagcgc cagtacgcca aggccgcccg
    13621 gacagcgggc cagaccgcag gcgcggcggc acagggcgcg gccaagacag ccggggcctc
    13681 cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg
    13741 ccactggaaa ggggcgctgg ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg
    13801 cggcttacag tcctgcaccg ccatgttcgg cagcgccgga agcggcatca cggcgtcctc
    13861 ttacttttcc gaggatagcg atatgttggc cgccgaggac gcctacgcgg agctggaagc
    13921 cgagctgcaa gactatctgg acaactacga ggccacccat gactatgacg aataccattt
    13981 tgaccttgac gagatcagcc atgaccccta tgtgctgatt tccattctgt cggcgctcca
    14041 tgacgggata tttaccatcg gggaggtaca gggtgatttg tccatgctct ttgacaagca
    14101 atatatcctg acagaaaacg tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg
    14161 gacggacgcg gagggcaaca cccatacaga cacctaccaa gtgccgtatg actattacat
    14221 ctgctacgtc acgctggaaa acttcgacct gtcccatgtg ccagtctata tcatggacga
    14281 ggaaacgcta agtttctatt ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt
    14341 ttccggcagc cagtacccca acgcctccac cctgacgccg ccgacctact acgacgtacc
    14401 cccggaggct ttggaggatg ccacctttgc cgccatgctc aaagaggcgg aaaaatatat
    14461 cggttatccc tatgtgtggg gcggcagctc tcccagcacc agctttgatt gcagcggctt
    14521 catttcgtgg gtaatcaatc atagtggatg gaacgtggga cggctggggg cgcagggcct
    14581 atataacatc tgcacccctg tttccagttc gcaggccaag cccggcgacc ttgtattttt
    14641 caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt
    14701 gatgctgcat tgcgggaacc cgatttccta cacgaacctt acttcaaact actggcaatc
    14761 ccatttttac agttacgggc gtttacctta acgccagaaa ggagctttca attatggcaa
    14821 ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa gtctaagatc gcggagtttc
    14881 agaaacagct tcgggagctg gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc
    14941 agcttgtgcg cggcatgaac atgaagccgg aggaatttgc cgcgttctta cgcagcggcg
    15001 caatgcaggc cgcccctgcc gcaacccctt accatgaaca ggaggacaat gcccatgaag
    15061 aataagacca gacggatttc ccgtattctc cccgcacttc tggccgtgat gctctgcgtc
    15121 acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg
    15181 gaggctacaa ccgaacccgc tacgggcggt ttggagcctg aaacagacga aaccggcacc
    15241 gccacggacg ggacagaaac cgaggccgaa aaagataccg gccctctgac cggcaaggac
    15301 atcaccgacc tgttttccgc gctgttcggt tccaaggtga gcattgccgc cacggacgac
    15361 ggcatccaga tcaccaccgg caccgacaaa aaagaacctg aacagaccgg caccgtcacc
    15421 accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc
    15481 cagcttccca atgggacgac ggttgaggta gtcggcacag acggcgactg gattaaaatt
    15541 ctcttgccgg agcgcatcgg ctatgtccat tccgactata tgaccgtcag cgaaaaggaa
    15601 gtagcggcta ccggcgaggg cggtttttcc ctctccattg acccggagga aattgcttct
    15661 ctgctggaac tgttcaatgg cggcggggcc agcgccgccc tgtccccgga cgggaacctg
    15721 tcactgattg acgacatcgg cagcgccgcg aaaaatggca agcagtttat caccgtggaa
    15781 accaaaaacg gcaatgtgtt ttatctgatt attgaccggg acgacgaggg aaaagaaact
    15841 gtccatttct taaatcaggt ggatgaagcc gatctgctga cgctgatgga ggacggggaa
    15901 accgtcaccc ccgctgctgt ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac
    15961 acgaattgcc cggtatgtaa aaccaacctg accgagtgca gcggcccgga gccgcaggaa
    16021 ccccagccgg aggaacccga agccccggag gaagaaccca agggcggcgc gggcggcctg
    16081 atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg ccctttacta tttcaagttc
    16141 aaaaagccca aggctgaaac caccggcagc gacgatttgg gcgagtatga ttttggggag
    16201 gacgaggatt tggaggacga ggaacccgaa accgagtttg acccggaagc tgattttgtg
    16261 agtgaacagg aggacgagga atgacatata aacttgtaat cgcggaaaaa cccagcgtgg
    16321 cccgcagcat tgcgggcgtg atcggggcca cggagaaaca cgacggctat ttggagggga
    16381 acggctatct ggtgagctgg tgcatcggac atctggtgtc ctttgccgac gccggacgct
    16441 atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc cattctcccg gaaacatggc
    16501 agtacatcgt ccccgacgac aagaaacagc agtttgacgt tctctgttcg ctgatgcagc
    16561 gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt
    16621 tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt tgagcgcctt tggatttcct
    16681 ctatggagga cagtgctatt aaggacggct ttgcccacct gaaaccgggc ggcgactatg
    16741 atccgctgta tcaatcggcc ctgtgccggg ccaaggccga ctggctgatc ggcattaacg
    16801 ccacccggct tttttccgtc ctgtaccaca agaacctgac tgtgggccgc gtccagacgc
    16861 cgaccttaaa aatgctggtg gacagggacg ccaagattac cgatttcaag aaagagaaat
    16921 atcacatcgt ccatatcacg gcgggcggcg cggacgcggt aagcagccgc ttttccgacg
    16981 ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg
    17041 tgacgcggga gaaaaagacg gaacagccgc ccaagctcta tgaccttacc accttgcagc
    17101 gggaggctaa ccggcttttc ggctataccg ccaagcagac ccttgactat gcccaggcac
    17161 tgtatctaaa gaagctgctg acctatccga gaacggatag tcaatatctt accgacgata
    17221 tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga gctggtgccg tttgccaagg
    17281 gcctgaacct ttccccgcag tttgaccgca tcctgaacag caaaaaggtg tccgaccacc
    17341 acgccattat ccccaccgct gaatttgtga agcagggctt tggcactctg gccgagagtg
    17401 aaaaaaagct gctgtccctg atctgctgca agctcttatg tgccgtggcc gggccgcata
    17461 tctatgaggc cgtcaccgcc acatttacct gtgccgggca ggagtttacc gccaagggca
    17521 agaccgtcct ttcccccggc tggaaagaga ttgaacgccg gttccgttct tccttaaaga
    17581 ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc ggagctttgc gagggccaga
    17641 cctttgcaga cgtggccgcc tctgtcaccg agcatttcac cacgccgccc aagccctaca
    17701 ccgaagatac gcttttgtcc gctatggaac gggccggggc cgaggatatg ccggaggatg
    17761 ccgagcgcaa gggattaggc acaccggcca cccgcgccgc cattctggaa aagctggtgc
    17821 agatgggctt tgtccagaga aaaggcaagc agctcattcc caccaaggac ggcatcaatc
    17881 tggctgtggt gctgccggag gcgcttactt ctccccagct tactgccgaa tgggaaaacc
    17941 ggctgaccga gatcgccaag gggcaggccg atcctgacga atttatggac ggcatcgaga
    18001 cgcaggcgcg ggagctggtg cagacctatt cctgtatcag tgaggaaaag cagaaattgt
    18061 ttcaggccga gcgtgttcct atcgggacgt gtccccgctg tggtgaggct gtctatgagg
    18121 gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc
    18181 gattttttga ggaacgcaag aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa
    18241 ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac cggcaaaacc tacgacggga
    18301 cggtgctttt ggctgatacc gggggcaagt acgtcaacta ccgcattgaa cacaggagct
    18361 aaaggacagt aacacagcaa gcatagggag tgtggctaca ctcccggtaa aatcccccgg
    18421 ccgcgtccct gccgggccgg ggatttttgc ttgttgggga agtccccaac cccctatttt
    18481 ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag tattttctat ccaagtccat
    18541 aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc tggatttgcc caccacaacc
    18601 gaaacgctgc aagccgccct gcgggatgtt ggcattactg ccgacaatcc gcaggatttt
    18661 tttatcaacg ggtattcctg cccggaggac agacaccttg ccattcccta tgacatggtg
    18721 ctggccgccg acgtggacga gctgaatttc cttgccgccc ggctgggcca gcttgacgcc
    18781 gccgagatcg cagagctgaa cgccgccctg caaaatcccc gtggcggctt tgagaatatc
    18841 ggccagatca tcgactacac cgaaaatgtg gattactatg tgcatcttcc cgacgttagg
    18901 ggggatggac agttgggcga ctactacctg aaccgttccg gcatggtgga tatgccggag
    18961 gaatggaaag ccgggattga tacggcgcag tttggacgcc acattgccca gcaggaacaa
    19021 ggcgttttta cccagtacgg ctatctggtg agaagcggcg acgaatggca gcgcgtccat
    19081 gagggccagc cggtgccgga ggaataccgg gtgatgcact acccccagcc ggaagctgac
    19141 cgggaggaac cgcaggccca gcagccggag gcatccgaaa gccccgacaa agcgccgccc
    19201 ccggttatcc cgatcatgct ggacggcatg aacagcaaag aacgcatgaa agtgattact
    19261 gaccgtctgg aaaccggcgt acaggagctt tttgaaagta gccgctatat ggcttactta
    19321 tccgctatgt ccaagatgca cgattacagt ctgaacaata cgctgctgat cgccatgcag
    19381 ggcggttcga ttgtggcggg ctataacagg tggcaggatg aatttgaccg ccatgtgaag
    19441 cggggcgaga aaggcattaa gatactggcc ccggccccgt tcaaggttaa gcagcagatg
    19501 gagaaaaccg acccggccac ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc
    19561 gaagaacagg aagtgacggt tccggccttt aaggtggtga cagtctttga catcagccag
    19621 accgagggca aggattttcc catgccctat atcagtgaac ttaccggcga cgtgcagcag
    19681 tatcaggatt tctttgccgc gctggaacgg acatcaccct atcccataag cattgagccg
    19741 atttcagacg gggccaaggg ccgctgcttc tacgcaaaac agcgcattgc catcaacgag
    19801 ggcatgagcg aattgcagac cctaaagacc gccatccatg agattgccca tgccaagctc
    19861 cacgccattg accgggaggc ccccgccgag gaacaggcgg cccggccaga ccgcagcacc
    19921 cgcgaggtac aggccgaaag cgtggcctat accgtctgcc agtattacgg gctggacacg
    19981 tcggactatt cttttggcta tgtggccgat tggagcagcg gcagggagtt atccgagctg
    20041 aaagcctccc ttgaaaccat ccgggccacg gccagcgaat tgataaccga gatcgacggc
    20101 cattttgccc agcttcagca ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt
    20161 gacgttaccc cggaggcagc ggccccggcg caggccgagg aagaacaggc cccggccctt
    20221 gatccggcag ccgagccgct tgtgactgtg ctatggagcg aaagccccca gctaaaggac
    20281 ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg cccgtctgga tgcagcatat
    20341 ccggcagagg caggctatga caaaaccgcg ttccgcattg actgtatctt tcagggcgag
    20401 ccggacagct acgaggggcg gcaggatttt ggagataggg acggttccct gatcgaacat
    20461 atccagagct accaccaatt ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct
    20521 acaaccggca tagagggctg ggagcaggac aaggcccatc gggatatgct gttaggcgag
    20581 tttgtcccct atatgaagct gcattgcagc cttgcccaaa tggaacagac ggcgcagggc
    20641 cgcttacagt ccggggaaac cctgacgccg gaggaaacgg cctatttggg ggcgcttctc
    20701 tcttatgtgc aggagtgccg ccccatgctc aatcagggcc agtatcagtt gccggagccg
    20761 ccgaaactgg ctgacttcga ccagagcttg caggactaca aagcacaggt acaggccgag
    20821 atcgcgcagg aggccgccgc agccggtatg accgtggagg aatacgcggc ggctggctat
    20881 gaagcacccc aacaggacac cttttccatc taccagcttc gaggcgggga ggaaacccgc
    20941 gactatcggt ttgagccata cgagcgttta caggccgcag ggctggccgt ggacagtgcc
    21001 aactatgacc ttgtttatac ggcaccgctg gataaaagca cgtctttgga ggatatttac
    21061 cgcacattca acatcgacca cccggcagat tttaggggac acagcctttc cgtttccgac
    21121 gtggtggtgc tgcaccagaa tggacaggac accgcccact atgtagacag cttcggctat
    21181 atggaagtgc cggagttttt gcgggaaaat cccctgcgta ccgccgaact gtctacggag
    21241 cagaacgaaa acatgattga cggcattatc aacaatacgc cgcccgaacc gcagccggag
    21301 gaacccgaac agctatcttt tgaggatgtg gcagttgctt ccggccagac acaggaggaa
    21361 cccccggagg ctgcgccccc gcaaacggct gtccgctatt atcccatcaa tgagggagcc
    21421 gcccgccgcg ccaaggaagc catgagcttt tctgactacc ggcccggcag cgccacggcg
    21481 gaataccggc actatgtcga tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg
    21541 gagccagagt atcacgaaaa gatagacagc ctccttgaca cctacgcccg caagctggcc
    21601 gagaacatga ataagggcta tgagattacc gcccgcgtcc cctctgtcct gattgccggg
    21661 ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg
    21721 gaggaatttc agtacattca gggcttgctt gacaaaatcc gcagcaccgg catgggtggt
    21781 atcagcgccg acgatccgca ggccgtttcc aagctggaaa agaaactgga aaagctggaa
    21841 gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc gcaagcatgg gacgctggac
    21901 ggatgccccc atctgatcga aagaggcatt gaaaacctga aagcggatat ggcgtcgggc
    21961 tggcactatg agaaaaaacc gttccagtcc tggcagctct ccaacaataa cgccgagata
    22021 cgccggttaa agggccgcat tgaggaattg acccgccaaa aggaggcggc ctatgtggga
    22081 tgggagtttg acggcggcac tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc
    22141 tttgagggga agccggatgc tgctgtacgc gacgagctga aaagcaacgg tttccgctgg
    22201 tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac
    22261 cgtatcaagt gcatccagcc cctttccggc gaaaagccca gcgccttaca gcttgcggcc
    22321 cgcagggaga aagaaaagcc ctctatccgg gcgcagctca acgcggccaa gaccgaacag
    22381 gccaagcagc caccggcgcg ggaaaaatca aagggattgg aggtagagta aatgcagaag
    22441 tttgaaaatg tggacgttct ggccgcgctg gaacagatca tgcggcagaa caccgcgttt
    22501 tatcaaagtg actttggcat tgacaaagac attatcaggc gggatgccgc cagcgatcag
    22561 gccgcagaca agaccctgct ctggatgtcc cggccctccg ggacgtactg tttccgggaa
    22621 cgtgatgttt tcctgaaaga cacccgacag tacaacacat ggaaatttta cggggagcag
    22681 acccgtgaca agatactggc ctacgcggtg gagctgaccg gcacacaggg cggcactatc
    22741 cggggcaatc tctatgagct ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc
    22801 cagccggtga gtgtgaaccg gctcttttat gagcatggca cccgtgacat cccggagagc
    22861 cagcgttttg acggaagccc tgaccgggta ttgggaaact ttttgtacta tgaggcccag
    22921 ccccatgacc cggctgtgct gcaggaggcg ctacggacgg agcagcacag ccgggggcgg
    22981 gccgtccccg gcgatttcaa agtccacgtc accgctttgc atgacagccg gattgaaacc
    23041 gaggcccggc gtattgtgga cagcctgaaa gatctgtccg cgcctaacag ccccaataaa
    23101 acgcatttca tggtggagat ctccccgcac tttgcggcga ttgcttccac caaggataat
    23161 gaccggctct ttgccatgct gccctataaa tccctgtgtt ttaccgggat gaaaggcagg
    23221 catggcgtgt atgccgtgat taaccgggac gagcgccggg atgtgagtat tcgcaggccc
    23281 cggccctcta tccgcaagca gctatccgac agcaaacagg ccgccagccc aaaaaaggcg
    23341 gcggccagaa ccaaaaagaa tgaattggag gtataaggaa tgatgcactt taccgtggag
    23401 gaagaaaacc tgatctgtat gtaccacaat gcagaccggc gcagaacgat aaacaggctc
    23461 accgccgtca tgccggttat ggatggggat atgcgggcgc aggcccagca gaccctttcc
    23521 aagctggaac gcatgacgga tgccgactat gacggccaga agttccactt cacagacgaa
    23581 taacgaacag cagacgccgg gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc
    23641 tttttgccta ttcctacgca tttagaaccc ggtattgtcg gggataggat gaagtattcg
    23701 cttgcccctg caaacatgcg ggagaagcct tgcagcgggc ggttttcaat cccttaaatg
    23761 cgtaggttgc agctataatt agacccctga aacagatata gccgcgcagc gggtgtatct
    23821 gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc
    23881 gtctatctct gttccagcgg gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc
    23941 tgccgtatat cttcgtcaat gactttcttc tttttgccgt ttgcctgtct gatctccacc
    24001 gtggaaagct cgttgatgta gtcagcataa tgggccaaca ctttttccgt ggcccacctc
    24061 tcaccggcaa cggcggcttt gatcgtttca tagtccagca gcttttcttg tccgtccatc
    24121 ctgatacccc cgtcagactg attttttcta tgataccata ttcggaagca tggaaccaga
    24181 gaaaacggcg cttttctgct ggtaaattct acgcatttag aacagcactt cggcggggat
    24241 aggataaaac acccacatac cccgctgacc atgccggaaa agccttgaaa tataagggct
    24301 tttacagctc taagtgcgta ggtttccagt atcttttccg ctggcgctcg gcctctttgc
    24361 ggcggcgcat ccgtttggag caggccgcgc agtatttggc ccggttagac ttcggcgtga
    24421 acgtggcccc gcactcacag cagcgtttca cttcgtcccg gtctttggtg atctcggcat
    24481 acagggccgc atccagcggg aggacagcgg ccttaaacca gcggcacaga agcgaatagg
    24541 aaatgctctg tacgcaaata cattcttcgc cgtcgtccag caacaggcag ttgccgtcac
    24601 aatagttgca gcactcatgc gtcagcttcc gggccttgcg atattgaggg taattcattc
    24661 gtggaatact catagctctt gcgtccattc ctttcccggc gtcatacgca aaatgccgtc
    24721 cacattctgc ttgatgatgc cgtattcctg catctgcttt ttcgcttcgc cgtacttctc
    24781 atacagccgt tccttttcct cggccaatcg cttgtactcg gctttcaggg cgtacagatc
    24841 ggggagcttt ttcacaccct gtccctttag ggcctttgcc gcagcttcat acaaaatcaa
    24901 cgtgctttca tgctcacgcc ggaattttgc cttatccgct gcattgcggt attccatagc
    24961 cacgggccag agctgcttgt aggtagaaat attctttatc agcaccgcca tatccgcaag
    25021 ccggtgttcc atagctttga gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc
    25081 cactttgctt tccaaatccg ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt
    25141 ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc tcatagcctg ccgactgctg
    25201 ggccttaatg ttgttttcca gatcaatccg caaagaaatc cccttgcgct ctttgggcgc
    25261 tctggtgata acgcggcctt tgatacgctc tttgatggct tcctcggtgt aggcttcccc
    25321 caatgtcttg cagcgggtaa agcgttcctg tccgggagcg cggaaagaaa taaactttcc
    25381 cggcttgatc tcatagccct gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc
    25441 ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct ttccagctcg tccctttccg
    25501 ctgggcgtcc cactcggcat agctcttgcc cttactctgg ccgggcttca ccacggacaa
    25561 tccgttttcc ttgcacagcc tgtcgctggt gcgccggata taggcatagc tctgtttgtt
    25621 ggaaatgtac ttgcgctgat ttaccatatc cacggcacag aaaataatgt gattgtggac
    25681 gtggccctta tcaatatggg tagtcagcac atagggatat ttcccttgca gcacttcctc
    25741 ggcaagctgc ttcccgatct catgggcctg ttccggcgtg gcttcgccgg gggcaaagga
    25801 ttgtatcagg tggtggccca gattattgcc cttttggtat gcctgcgcca gcagcatttc
    25861 aaattcaatg tccgctgttt cataagagca ggcaaaggag gccaccagca gcttgccgtc
    25921 agttttgtcc gggttctcga tgtagtcaag ggcttttttc agcgttgact tgataggatg
    25981 tatctttgta actgccatat ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg
    26041 tacaccggcc ccataccgtt cagcctcttt gcaatctgat tgacgttgac gccgatcttc
    26101 tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca tgttgacgat atagccgtca
    26161 atgagcatct tccgggcgta ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt
    26221 tcaatcatag cccgttcctc cggcgtcaca gggacgcgca gcacgatttt tcgctttctg
    26281 tttgccatgc ttcaccgtcc tttccattca ggggtttggg gatttcccca acaagcaaaa
    26341 cggacgcggc ccggcaggga cggggcaggc cgtttttccg ggagtggcta cactccctat
    26401 gcttgctacg ttactgtttc tccactaaca agacgtttcc aaagccccaa actacccgca
    26461 aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg
    26521 ctaaaagtaa aacagaggat gccagttaca catcctccgt ttcattcttc ttcggctttt
    26581 caattagtat ggcgctgtca atcgcgccct cgatgatcgg caggtattct tcggggcaga
    26641 gtttcagctt gtggctgacc ctttgccgca gttcgctttc ctcccgcatg atctccgggt
    26701 taaagtagcg ttccacagga agcccgcagg ctttaataag ctgtatgaca accggcagac
    26761 tgggaatggc ccgtcgtttt cgatgttggc aagatagcgc cagtcaatat ttattttctc
    26821 tgccattgct ttgcgcgaaa cgcgctttgc ttgccgggcc gctttcacat ccgcgccgaa
    26881 agtttcaaag ccgggacaat cttcaatatt cgccatatcg cacctcccac ttacattgta
    26941 tatttcatac tctgcccgtg gaatgttggt atatgcacct ttcttaaagt ttatatttca
    27001 cattttgttt cttttattct tcgagcaaaa gaattataat gtaccctgtg gaggtgtact
    27061 atggactaca tgacgttgaa agaggcaggc gagaaatggg gtgtgccccc acgatggata
    27121 aactactatt gtgctgcggg acgcatcccc ggcgctgtaa agatggcgac gatctggctc
    27181 attcccaaaa ctgcggaaaa gccgattgac aggcgtacaa agaaaggaaa ggcggttact
    27241 gaatagtata atgaaaccgg cgcattttgg atatggtttt gaagagagag aaagcatggt
    27301 attttgtcac ggaaaaacta taagatgata gatggcaggc tgctccagac cgataaaaag
    27361 tattctcatc tgaaacagaa agaaaagatt tacacatgga tgtatgaaga agcgaaactt
    27421 tatcatgaca gaaatggaaa atgcccgaaa caaagacgag gaagctgctg ctgtatcagc
    27481 cgtatatggt agaattgaaa aggccggcat ctggattctt tatggtgaag tctccagaca
    27541 ttggataact gctggtctaa tgcgcctttg agtatggggt gattgatttt aataccgggg
    27601 aggtacggct ttttgaggaa aatattctgg gatttacatt tggcgattat tacatttcct
    27661 gtaactgcaa ggacacggat gatgaagata tgaggaatat agacttgctg attttctact
    27721 gtcttgaacg gtattaggac tgaatctgct gcgcggagtg cggcaagagc gaccggaggg
    27781 agtacacaca ggccctgcct gtgtgaaaag ccataccgca aggtgcattc gcaaaaaaga
    27841 ggccgcgcat tttgctgagg cttggggtgt ccccaacaag caaaacgaga acgccgcagg
    27901 cgggcaagtg gtggatcaga atatccgtaa aggatattct gatccattgc ttgccagtat
    27961 tatcgggatg ttttgacaac cgctgtaatc gaaacatccc aaacattaag aaggtgttaa
    28021 ttattattat cttgatttaa ttcgtttaaa ccaataatat agatacatac accatgaaaa
    28081 gaagcgagta tcccaatgac ggatgattaa aatccgaaaa gaaatcgctt tgaaactaca
    28141 gggaaactac agactgttat gttatcttct taaaatggag ggatttttat gtcgatacga
    28201 attctacttg tcgaggatga tgatcatatc tgtaatacag taagggcgtt tctggctgag
    28261 gcaggatatc aggtggatgc ctgcacagat ggaaatgagg catacaccaa gttttacgaa
    28321 aacacttatc aactggttat tcttgatatt atgctgcccg gtatgaacgg gcatgaactt
    28381 ttgcgtgaat ttcgtgcgaa aaatgatact cccattctga tgatgacagc cctgtcggat
    28441 gacgaaaacc aaatccgggc gtttgatgca gaggcagacg actatgtaac aaagccattc
    28501 aagatgcaga ttttactaaa gcgggtggaa gccctgctgc ggcgcagcgg tgcgctggca
    28561 aaggaaatcc gtgtcggcag gctgacactt ctgccggagg atttcacggt actttgtgac
    28621 ggtacagagc tgcccctgac acgaaaagaa tttgaaatcc ttttgctgct ggtgcagaac
    28681 aaaggcagaa ccttaacgca tgaaatcatt ttgtcacgta tatggggata tgactttgag
    28741 ggtgatggca gcacagtcca cactcatatc aaaaatttgc gggccaagct gccggaaaat
    28801 atcatcaaaa ccattcgcgg tgtaggatac cgattggagg aatcataata atggaaagaa
    28861 aagggatttt cattaaggtt ttttcctata cgatcattgt cctgctactg cttgtcggtg
    28921 taacagcaac actgtttgca cagcaatttg tgtcttattt cagagtgatg gaattacagc
    28981 aaacagtaaa atcctatcag ccattggtgg aactgattca gaatagcgat aggcttgata
    29041 ttcaagaggt ggcagggctg tttcactaca ataaccaatc ctttgagttt tatattgaag
    29101 ataaagaggg aagcgtactc tatgccacac caaatgccaa tacatcaaat agttttagac
    29161 ccaactttct ttatgtggta catagagatg ataatatttc gattgttgct caaagcaagg
    29221 caggtgtggg attgctttat caagggctga caattcgggg aattgttatg attgcgataa
    29281 tggttgtatt cagcctttta tgcgcgtata tctttgcgcg gcaaatgaca acgccgatca
    29341 aagccttagc ggacagtgcg aataaaatgg caaacctgaa agatgtaccg ccgccgctgg
    29401 agcgaaagga tgagcttggc gcactggctc atgatatgca ttccatgtat gtcaggctga
    29461 aagaaaccat cgcaaggctg gaggatgaaa tcgcaaggga acatgagttg gaggaaacac
    29521 agcgatattt ctttgcggca gcttctcatg agctaaaaac gcccatcgcg gctacaagcg
    29581 ttctgttgga gggaatgctt gaaaatatcg gtgactacaa agatcattct aagtatctgc
    29641 gcgaatgcat caaaatgatg gataggcagg gcaaaatcat ttccgaaata ctggagcttg
    29701 tcagcctgaa tgatgggaga atcgtaccca tagctgaacc gttggacata gggcgcacgg
    29761 ttgccgagtt gctgcccgat tttcaaacct tggcagaggc aaacaaccag cggttcgtca
    29821 cagatattcc agccgggcaa attgtcctgt ccgatccgag gctgctccaa aaggcactat
    29881 ccaatgtcat attgaatgca gttcagaaca cgccgcaggg aggcgaggta cggatatgga
    29941 gtgagcctgg tgctgaaaaa tgccgccttt ttgttttgaa catgggcgtt cacattgatg
    30001 atactgcgct tccaaggctg ttcaccccat tctatcgcat tgatcaggcg cgaagcagaa
    30061 aaagtgggcg aagcagaaaa agtgggcgaa gcggtttagg acttgccatc gtacaaaaaa
    30121 cgctggatgc catgagcctc cagtatgcgc tggaaaacac ctcggatggc gttttgttct
    30181 ggctggattt accgctcaca tcaacattgt aaatatttaa aacttaaatg attttgggct
    30241 ctttgtcaaa taggactgat agagtgagat aagaacatct aaggt
//
LOCUS       DAYYNQ010000025        30120 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000025 DAYYNQ010000000
VERSION     DAYYNQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.025"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..2414)
                     /locus_tag="ACHBJ1_001016"
     CDS             complement(51..2414)
                     /locus_tag="ACHBJ1_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM9429979.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2579..3083
                     /locus_tag="ACHBJ1_001017"
                     /pseudo
     CDS             2579..3083
                     /locus_tag="ACHBJ1_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3191..3538)
                     /gene="rbfA"
                     /locus_tag="ACHBJ1_001018"
     CDS             complement(3191..3538)
                     /gene="rbfA"
                     /locus_tag="ACHBJ1_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM9429980.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3565..5871)
                     /gene="infB"
                     /locus_tag="ACHBJ1_001019"
     CDS             complement(3565..5871)
                     /gene="infB"
                     /locus_tag="ACHBJ1_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM9429981.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5884..6195)
                     /locus_tag="ACHBJ1_001020"
     CDS             complement(5884..6195)
                     /locus_tag="ACHBJ1_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM9429982.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6192..6485)
                     /locus_tag="ACHBJ1_001021"
     CDS             complement(6192..6485)
                     /locus_tag="ACHBJ1_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM9429983.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6507..7682)
                     /gene="nusA"
                     /locus_tag="ACHBJ1_001022"
     CDS             complement(6507..7682)
                     /gene="nusA"
                     /locus_tag="ACHBJ1_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM9429984.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7706..8179)
                     /gene="rimP"
                     /locus_tag="ACHBJ1_001023"
     CDS             complement(7706..8179)
                     /gene="rimP"
                     /locus_tag="ACHBJ1_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM9429985.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8323..12675)
                     /locus_tag="ACHBJ1_001024"
     CDS             complement(8323..12675)
                     /locus_tag="ACHBJ1_001024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM9429986.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12887..14596)
                     /locus_tag="ACHBJ1_001025"
     CDS             complement(12887..14596)
                     /locus_tag="ACHBJ1_001025"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM9429987.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14664..15932)
                     /gene="rseP"
                     /locus_tag="ACHBJ1_001026"
     CDS             complement(14664..15932)
                     /gene="rseP"
                     /locus_tag="ACHBJ1_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM9429988.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16093..16893)
                     /locus_tag="ACHBJ1_001027"
     CDS             complement(16093..16893)
                     /locus_tag="ACHBJ1_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM9429989.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16890..17702)
                     /locus_tag="ACHBJ1_001028"
     CDS             complement(16890..17702)
                     /locus_tag="ACHBJ1_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM9429990.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17897..18454)
                     /gene="frr"
                     /locus_tag="ACHBJ1_001029"
     CDS             complement(17897..18454)
                     /gene="frr"
                     /locus_tag="ACHBJ1_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM9429991.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18457..19179)
                     /gene="pyrH"
                     /locus_tag="ACHBJ1_001030"
     CDS             complement(18457..19179)
                     /gene="pyrH"
                     /locus_tag="ACHBJ1_001030"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM9429992.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19315..20196)
                     /gene="tsf"
                     /locus_tag="ACHBJ1_001031"
     CDS             complement(19315..20196)
                     /gene="tsf"
                     /locus_tag="ACHBJ1_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM9429993.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20295..21077)
                     /gene="rpsB"
                     /locus_tag="ACHBJ1_001032"
     CDS             complement(20295..21077)
                     /gene="rpsB"
                     /locus_tag="ACHBJ1_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM9429994.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21436..21915
                     /locus_tag="ACHBJ1_001033"
     CDS             21436..21915
                     /locus_tag="ACHBJ1_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM9429995.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22132..23223
                     /gene="phnW"
                     /locus_tag="ACHBJ1_001034"
                     /pseudo
     CDS             22132..23223
                     /gene="phnW"
                     /locus_tag="ACHBJ1_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23216..24001
                     /gene="phnX"
                     /locus_tag="ACHBJ1_001035"
     CDS             23216..24001
                     /gene="phnX"
                     /locus_tag="ACHBJ1_001035"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM9429996.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24347..26038)
                     /gene="argS"
                     /locus_tag="ACHBJ1_001036"
     CDS             complement(24347..26038)
                     /gene="argS"
                     /locus_tag="ACHBJ1_001036"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM9429997.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26460..27407)
                     /gene="arcC"
                     /locus_tag="ACHBJ1_001037"
     CDS             complement(26460..27407)
                     /gene="arcC"
                     /locus_tag="ACHBJ1_001037"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM9429998.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27522..28541)
                     /gene="argF"
                     /locus_tag="ACHBJ1_001038"
     CDS             complement(27522..28541)
                     /gene="argF"
                     /locus_tag="ACHBJ1_001038"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM9429999.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28632..29861)
                     /gene="arcA"
                     /locus_tag="ACHBJ1_001039"
     CDS             complement(28632..29861)
                     /gene="arcA"
                     /locus_tag="ACHBJ1_001039"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM9430000.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8051 a         6463 c         5351 g        10255 t
ORIGIN      
        1 caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt ttattggtca
       61 agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc acgaatatag
      121 ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc ttgacttcct
      181 ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt tactacacgc
      241 atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat taaatgatgg
      301 ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa agcaaataag
      361 atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc acgcggatct
      421 tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat ttttagtttt
      481 gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga ttccgttcct
      541 gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca atcaatgatt
      601 ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg agcacgcgga
      661 tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt atccatgact
      721 gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc cttcttttct
      781 gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc ttgctggaag
      841 actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga aaggtcatgt
      901 gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg caagaatgat
      961 tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa gaacccttca
     1021 tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag ccattcatca
     1081 ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac ttgattcaat
     1141 cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt tttctcaatg
     1201 atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga gcctggtttt
     1261 tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg atcgattcca
     1321 ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat ttctctgatt
     1381 tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga tttcagccaa
     1441 cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg tacttggtgt
     1501 gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt ccatcctttt
     1561 ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc tgtcttcagg
     1621 cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt ttcagccata
     1681 acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca gccaagtccg
     1741 ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg gttggctatt
     1801 tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg attgataaag
     1861 acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc accaatcacg
     1921 acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga aagagagtat
     1981 ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg agaagcaatg
     2041 cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc ttccgttact
     2101 tctggataga tctctgtgta acttccatct aaataagcat tcgataccat gacttgtccg
     2161 ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat gactcggttc
     2221 aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat tggatgtttt
     2281 ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag ataaagttgc
     2341 ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa gtattcttta
     2401 ctagaataat ccatacataa cactcctttt cattattact cttattctag cgcagatact
     2461 cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat ctagccaaat
     2521 gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac attgatccaa
     2581 aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca tcatgcataa
     2641 tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct ggatcgagca
     2701 attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt caatatgtga
     2761 attggtatcc cagctactat gctaatttct acagggatta tccaaaattt ggatcgtgac
     2821 caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt cttttaactg
     2881 tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt ctagttactt
     2941 ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc ggtatcttga
     3001 tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa ttttttactt
     3061 ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg gaactcttag
     3121 gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca ggatttgcgg
     3181 gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg ttcccataga
     3241 caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt agattgtgcc
     3301 ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct tttttcttat
     3361 ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag tcccccgtaa
     3421 ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt ttgcgcaaaa
     3481 tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag ttagccataa
     3541 tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga taaatccttc
     3601 aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac attcataacc
     3661 cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt ggccttcata
     3721 gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat atccttctgt
     3781 tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac gaacgatcat
     3841 ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg cagtttcgat
     3901 ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt cagtatcagc
     3961 ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag cgttgctggc
     4021 tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga cgatttttac
     4081 acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg ctgaaccttg
     4141 tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca ggctttcaaa
     4201 taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg cacgttttgc
     4261 gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga aacggtcacc
     4321 tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc ctgcttcttt
     4381 atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat taccgacaac
     4441 gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca caggaccttt
     4501 acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg gatctgcttt
     4561 taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga tgttttggcc
     4621 gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag gaattaattc
     4681 atactcactt aattcttgca taacatgctg aggatttgca cctggtttat caattttatt
     4741 tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt caactgtttg
     4801 tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga tacttgctcc
     4861 acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag tgattggttt
     4921 accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg cttcgccgct
     4981 tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt ctacgtgtcc
     5041 cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt cttctgcctc
     5101 gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt ccatgccata
     5161 gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat tgaccatgac
     5221 accaagcata aataattttt taatgatttc tgctggctca cgatgaattt ttttcgcaat
     5281 atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac ggaatttgcg
     5341 tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt ttttattata
     5401 acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt tgttgttatt
     5461 gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc tgctggtatt
     5521 tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac cgctcccttg
     5581 accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg ttgtttcttt
     5641 tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt gtgcagcttg
     5701 ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac tgctagtaat
     5761 cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca cgacgtcttt
     5821 actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca tgcaatcacc
     5881 ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca ttattccgaa
     5941 taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata gctcaaagca
     6001 aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct cacttgcatc
     6061 ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg tcagttcttc
     6121 acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg ctttttgccg
     6181 attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt gacatagtca
     6241 agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc gagaattttt
     6301 ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc tcgtccaggc
     6361 atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat tcggaccatc
     6421 tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat tttcctttgt
     6481 ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac cgtttcattg
     6541 ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat tgcttcatca
     6601 tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt catgtcagat
     6661 tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc attttgtccg
     6721 cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca ggcctttgga
     6781 tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt tgcgatgaat
     6841 acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa ttcattgacg
     6901 attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc gatatttgga
     6961 tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat gctgacaatt
     7021 tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg caataggtct
     7081 ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac tcgggaaaca
     7141 taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc ttgtttagaa
     7201 agaactgcct caattttacc aagattgaca taaatataac gtttatcctg gcgttcgacg
     7261 attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat cgttctttct
     7321 gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat ccgtccaaaa
     7381 tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg attgattaaa
     7441 agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt tacttctttt
     7501 accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac gttttgtgct
     7561 tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc gatcacgatt
     7621 tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat ttctttactc
     7681 atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct tttgcaatat
     7741 tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc aatgtaagct
     7801 gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct actggttggt
     7861 ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct tttttcaatg
     7921 gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc ggatctggat
     7981 ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct tcgatatcga
     8041 taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct tttacaaatt
     8101 cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt tctgtgactg
     8161 tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta attccattaa
     8221 aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata agaactatac
     8281 catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca tatcgaataa
     8341 cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca tatattcgat
     8401 caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa atggaccgtc
     8461 ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga cagctctaaa
     8521 tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt tatacaaatc
     8581 aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca gcactgtcag
     8641 ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt tcattgcttg
     8701 tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg cacgtacaga
     8761 aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga cacgaagagc
     8821 catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt ttgaacaaga
     8881 atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg tttctcgcaa
     8941 ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt taaatgccat
     9001 cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac agccgattac
     9061 ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta accatacgtc
     9121 tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag gatgcgtttc
     9181 ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg tccctgtttt
     9241 ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata tccgcatcac
     9301 ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt gaagcatccg
     9361 aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat cgtgaatcga
     9421 gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg gatattggat
     9481 cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc ctggatgctg
     9541 gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt ctgcacttcg
     9601 aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg ttttgtcggc
     9661 gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata ctttcgtata
     9721 gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg gcactttgtc
     9781 tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt ttaagcgtgc
     9841 accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg taccatcttc
     9901 aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg gattaacttc
     9961 agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg aacctactag
    10021 atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa taaccgagaa
    10081 gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga tctcaggtaa
    10141 cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt catcttctga
    10201 acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt cgcagatatc
    10261 tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc ctaaaaagtg
    10321 aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac tatgacgatt
    10381 caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg catcttcttc
    10441 gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt ttccgatttc
    10501 taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt cttgctcgat
    10561 caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt ttgctcggtt
    10621 tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct tatcacaagc
    10681 tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta ttcgtgggac
    10741 gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt tcagtcctgc
    10801 ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag aatctccttc
    10861 tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca ttgcctcttt
    10921 cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc ggtgatgctg
    10981 ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt tgaattgagg
    11041 atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt caggaatacc
    11101 ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt gggctaccaa
    11161 aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg actttgaccc
    11221 tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca atggatgtcc
    11281 tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt tgactgcggc
    11341 tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt caaatacgac
    11401 gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga caccatcatc
    11461 tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc cagcactatg
    11521 tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc ggtgtcccca
    11581 ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg tactcatatt
    11641 gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt tgcgaggtgt
    11701 atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa acgtatcttc
    11761 ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa atacttgttc
    11821 atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat ctgtgatttt
    11881 caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga atacgtaccc
    11941 ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg ttggttcatc
    12001 ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt gctcttttct
    12061 ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca taaaggcttc
    12121 tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt gctgctcatc
    12181 cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact cttctactaa
    12241 tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg gcaagatgac
    12301 tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc
    12361 gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt catcgaatgt
    12421 ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag cgatagtcag
    12481 atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat gtagcgtaaa
    12541 ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag cagtaagcag
    12601 tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt caaataaatc
    12661 tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc
    12721 ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt ttacattaaa
    12781 aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat cagattgtca
    12841 aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat tcgccttggt
    12901 tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc atttcgccag
    12961 ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca gtgattctga
    13021 ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca tttctgtcat
    13081 caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc gtcaggcttg
    13141 tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc gcaattcctt
    13201 ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat aataaacgac
    13261 ttacaccgat accataacag cccataatca caaatttttc tcgtccattt tcgtctagta
    13321 cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt ccgatttcga
    13381 ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct tcttgtacaa
    13441 aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca tttttatagt
    13501 ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca taacggtctg
    13561 catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac tcagcaccta
    13621 gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt
    13681 tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca ggttgttcat
    13741 ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag aaatctgcta
    13801 cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc tgtgtttcat
    13861 gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca taatcacttt
    13921 cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat tcctttgagt
    13981 ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag ccacaacgct
    14041 tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg
    14101 ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc aaaccgaagc
    14161 gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa ggtaatttct
    14221 tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa
    14281 ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca tatgtttcgt
    14341 aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc atttctaccg
    14401 ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat acacgatttg
    14461 ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca gctcttagca
    14521 agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca
    14581 tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat cgtttgttat
    14641 tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact
    14701 agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc ttctttttct
    14761 tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt cccgccatct
    14821 aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag cgccatcaaa
    14881 ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta catcatgaca
    14941 ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc tttgaaaatt
    15001 tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat aaaacctgta
    15061 ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc tacagatttc
    15121 ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc taatgtttta
    15181 cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc ttttccgtcc
    15241 acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc attaggcatg
    15301 atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa tgccagaatg
    15361 atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt ggtcagcata
    15421 cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct gatttctgtt
    15481 ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac ttctgtttcg
    15541 tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg gctcaattcc
    15601 atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc tacgatgccg
    15661 tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc atcatcccca
    15721 ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata tgttgtcccg
    15781 tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc gcggacgagg
    15841 attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac taatatacca
    15901 aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac ttgttttttg
    15961 tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc aaagacacca
    16021 atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca caaagaaacg
    16081 caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa acagcaaact
    16141 gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt ctttcacatc
    16201 ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat
    16261 aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg ctgggtatag
    16321 caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg caccttcaat
    16381 cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc gaccaaccat
    16441 atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa cagccaagct
    16501 ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc tgacaagcac
    16561 aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg ttcctaaaag
    16621 aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa gaaagaaaaa
    16681 ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa ctccttcaaa
    16741 acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta ccgctaagat
    16801 tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa acaagactag
    16861 cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc atcttctgtt
    16921 ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc ttcttcaaga
    16981 tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact atatgcgatc
    17041 tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat cagctcagga
    17101 tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg
    17161 atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg gacagcagtc
    17221 acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc agtattgttt
    17281 tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa tacgttttca
    17341 taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa cgtatcaaag
    17401 aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt ccagttttca
    17461 gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt ttttgtgact
    17521 ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg acggttttgc
    17581 gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg tccatcggta
    17641 ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac
    17701 attctttttc ctcctatact taaaagattc ccactatctt aagtattata gcaatatcta
    17761 actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt ttttcttttt
    17821 ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga tccgcccctc
    17881 tgtttttatg attttattaa acttccaata attctttttc tttatcagca gcgatcttat
    17941 ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat
    18001 catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc gcatcacgac
    18061 ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct ttagctaatt
    18121 cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt ccatcattgg
    18181 ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga agaatgctct
    18241 tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat gccatttggt
    18301 tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg ctagcgttcg
    18361 cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct tttttcattt
    18421 tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta cagttgttcc
    18481 gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat tgaagacaag
    18541 cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga cttgtaatcc
    18601 tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg catctacttt
    18661 cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa catctgcgcc
    18721 gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc
    18781 gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac ggcgaatata
    18841 tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta cacccacgtt
    18901 ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac ccatgtaatc
    18961 agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc
    19021 acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt cttggatgat
    19081 ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag cttctccact
    19141 caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc caatattttc
    19201 tattgccatt ttaccatact tagatgattt cgacactcta agactggttt ttcttgtttt
    19261 gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac agaattattt
    19321 tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga taccttcgcc
    19381 aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt atttttcaac
    19441 agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg ctttgaattt
    19501 cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt cgttcaatgc
    19561 ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt gtgattcgtt
    19621 tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc
    19681 agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca agtatccacc
    19741 aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga ttttttctcc
    19801 gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg ttttgatttt
    19861 cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa tttcttttac
    19921 taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta cttcaacgat
    19981 tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa tgcggtcatt
    20041 tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt tttccatgtc
    20101 gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag tcatttcgcg
    20161 tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt
    20221 ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct ttgaaacagc
    20281 tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc tacgatttct
    20341 tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg atcttcacct
    20401 tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac ggcacgaatt
    20461 gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt tgtatcaacc
    20521 atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg ttctttacgt
    20581 gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc gcctaagaat
    20641 ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg tagaacttca
    20701 aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg tttttggata
    20761 gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta ttgtcctgca
    20821 cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac gaataatgct
    20881 acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac taatttaact
    20941 gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata tttcttcatt
    21001 tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag taattgtttc
    21061 atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct cttcttatct
    21121 tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga atgtggattt
    21181 gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc gcaagagcaa
    21241 atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa
    21301 acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt ttaggtataa
    21361 aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg aaatctttcg
    21421 taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca agtcattgaa
    21481 gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta tggcgttacc
    21541 gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat
    21601 gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga gactaggact
    21661 gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt cgaacgagtc
    21721 cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc atctgtttta
    21781 gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac gatcatggtc
    21841 atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca tccaactgaa
    21901 gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt ttttattttc
    21961 aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca ttttttattc
    22021 aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga tgttaacaag
    22081 agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca tatgtataaa
    22141 ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat gctgatcgat
    22201 cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa aaaattattg
    22261 gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg tagcggctcc
    22321 tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt actgatttgt
    22381 tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa actgaactat
    22441 accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac acagctgatt
    22501 gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac ttccggtatt
    22561 ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt gtttatagtg
    22621 gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg gattgatttt
    22681 ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt tgtacttgct
    22741 aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact tgatctatat
    22801 gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc ccctacccat
    22861 acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg aatcgaaact
    22921 cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac agagatgggg
    22981 ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct ttaccctaag
    23041 gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg atttgtgatc
    23101 tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg cgagatctat
    23161 ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa cgttaatgat
    23221 taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta tggctcctgt
    23281 ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca aagaaatccg
    23341 cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc tcccttctgt
    23401 caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg tggaggaatt
    23461 gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta ccttgaaacc
    23521 cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg gttccacgac
    23581 tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag aagggtattc
    23641 accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt ttccttatat
    23701 gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg
    23761 agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag taggtgtaat
    23821 tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag agatggggaa
    23881 aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg ctgatatttg
    23941 tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga atccagaatg
    24001 acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa taagaacagc
    24061 caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc agatgcaagc
    24121 ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc
    24181 ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg tgttcattca
    24241 gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt tctcttttgt
    24301 ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac atcttatctg
    24361 gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact gcataaacta
    24421 gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga gcatagtatt
    24481 tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt tcgtattttt
    24541 ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact tcccagctgg
    24601 tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct ttttccaaca
    24661 ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct tcaaatcgaa
    24721 caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa
    24781 tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa tcagggtttt
    24841 tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct tctaataaga
    24901 cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa ccaaatggaa
    24961 tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct ttcaactgtt
    25021 taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag tcgtaagtac
    25081 gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact
    25141 tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct gcacctttat
    25201 cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt
    25261 agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag attttattga
    25321 attctttcat cgattcatca cggaaccatt gccataattg aatagcctct tcatcgcctt
    25381 cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct gtttcagcta
    25441 cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct ttaacagctt
    25501 cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt ccccagtcac
    25561 ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa ccgatcgaat
    25621 taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata ttaggagaag
    25681 acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa tgttcttttt
    25741 ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg aaaaagttca
    25801 agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt tctgctaaat
    25861 ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag gctggaaatg
    25921 caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact tgttccagtg
    25981 ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta ttattcatct
    26041 ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag cgacggcata
    26101 cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt gtcctaaaaa
    26161 aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa ttctccaagt
    26221 gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct agccaagaat
    26281 gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt gaattatatc
    26341 agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat ggcctaaagg
    26401 agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga cttaacaaat
    26461 taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat attttctaaa
    26521 gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc tgcctctact
    26581 ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt catttctgaa
    26641 acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata cacattgtct
    26701 actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt ttctgaagca
    26761 aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc tacaacagga
    26821 atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt ttcgatgaca
    26881 cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca tccacgtcca
    26941 gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc ttcttcttcc
    27001 gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt ttcatccaca
    27061 ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt cagtacttcg
    27121 cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac acatgtatct
    27181 aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa taggttaccg
    27241 acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat aaatttcact
    27301 aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc atccgtagac
    27361 aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc attcgtcctt
    27421 tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt gattttccct
    27481 caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg agggataaac
    27541 aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt ttctgcttgg
    27601 tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat ttcagtgatg
    27661 ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa agctggcaag
    27721 cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc catattgatt
    27781 tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc cattgatacc
    27841 catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc gtcggtaatc
    27901 atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat ttcttcttca
    27961 ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc acctgtgact
    28021 aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt gataccttct
    28081 actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt tggatgccat
    28141 tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc ctctaccatt
    28201 ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac tttagctgta
    28261 tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata ttcaggatgt
    28321 gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga tgttttttca
    28381 aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg ttttttcaag
    28441 tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt aaagtctttt
    28501 tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat tctcctttag
    28561 attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta agaccgccgt
    28621 caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca gcgtggacca
    28681 ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc atagctacga
    28741 agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg agcgattgcc
    28801 aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc tccattacca
    28861 gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc gtgtaagtta
    28921 tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac atccatctta
    28981 ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac cattgtaaat
    29041 acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa cactttttca
    29101 aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt cgcattagtt
    29161 cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc tccgccttcg
    29221 atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc aaatcttgga
    29281 tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc
    29341 ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt
    29401 ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta tagactgcta
    29461 gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac catttcttgc
    29521 gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga tgcaatatga
    29581 gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc aatcgcttct
    29641 gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt ctttgcaaag
    29701 ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa cagacgatcc
    29761 ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa aactgtcttt
    29821 aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg cctccttctt
    29881 tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt tgtctcattc
    29941 aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt ttttctattt
    30001 ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata tttattcatt
    30061 gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt agcgtttact
//
LOCUS       DAYYNQ010000026        29470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000026 DAYYNQ010000000
VERSION     DAYYNQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.026"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1148)
                     /locus_tag="ACHBJ1_001040"
     CDS             complement(<1..1148)
                     /locus_tag="ACHBJ1_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9430001.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYH"
     gene            complement(1165..2211)
                     /locus_tag="ACHBJ1_001041"
     CDS             complement(1165..2211)
                     /locus_tag="ACHBJ1_001041"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM9430002.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(2375..4102)
                     /gene="ptsP"
                     /locus_tag="ACHBJ1_001042"
     CDS             complement(2375..4102)
                     /gene="ptsP"
                     /locus_tag="ACHBJ1_001042"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM9430003.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(4102..4368)
                     /locus_tag="ACHBJ1_001043"
     CDS             complement(4102..4368)
                     /locus_tag="ACHBJ1_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM9430004.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            4847..7081
                     /locus_tag="ACHBJ1_001044"
     CDS             4847..7081
                     /locus_tag="ACHBJ1_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9430005.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            7229..7549
                     /locus_tag="ACHBJ1_001045"
     CDS             7229..7549
                     /locus_tag="ACHBJ1_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM9430006.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(7754..7978)
                     /locus_tag="ACHBJ1_001046"
     CDS             complement(7754..7978)
                     /locus_tag="ACHBJ1_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM9430007.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(8176..9756)
                     /locus_tag="ACHBJ1_001047"
     CDS             complement(8176..9756)
                     /locus_tag="ACHBJ1_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM9430008.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(10157..11533)
                     /locus_tag="ACHBJ1_001048"
     CDS             complement(10157..11533)
                     /locus_tag="ACHBJ1_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9430009.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(11661..12749)
                     /locus_tag="ACHBJ1_001049"
     CDS             complement(11661..12749)
                     /locus_tag="ACHBJ1_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9430010.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            12921..13355
                     /locus_tag="ACHBJ1_001050"
     CDS             12921..13355
                     /locus_tag="ACHBJ1_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430011.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(13433..13966)
                     /locus_tag="ACHBJ1_001051"
     CDS             complement(13433..13966)
                     /locus_tag="ACHBJ1_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM9430012.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(14060..15238)
                     /gene="mutY"
                     /locus_tag="ACHBJ1_001052"
     CDS             complement(14060..15238)
                     /gene="mutY"
                     /locus_tag="ACHBJ1_001052"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM9430013.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(15231..16028)
                     /gene="recX"
                     /locus_tag="ACHBJ1_001053"
     CDS             complement(15231..16028)
                     /gene="recX"
                     /locus_tag="ACHBJ1_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM9430014.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            16135..17517
                     /gene="rlmD"
                     /locus_tag="ACHBJ1_001054"
     CDS             16135..17517
                     /gene="rlmD"
                     /locus_tag="ACHBJ1_001054"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM9430015.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(17691..18269)
                     /locus_tag="ACHBJ1_001055"
     CDS             complement(17691..18269)
                     /locus_tag="ACHBJ1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM9430016.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            18591..19061
                     /locus_tag="ACHBJ1_001056"
     CDS             18591..19061
                     /locus_tag="ACHBJ1_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM9430017.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(19169..20395)
                     /locus_tag="ACHBJ1_001057"
     CDS             complement(19169..20395)
                     /locus_tag="ACHBJ1_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9430018.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(21149..21349)
                     /locus_tag="ACHBJ1_001058"
     CDS             complement(21149..21349)
                     /locus_tag="ACHBJ1_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9430019.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(21483..22091)
                     /locus_tag="ACHBJ1_001059"
     CDS             complement(21483..22091)
                     /locus_tag="ACHBJ1_001059"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM9430020.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(22176..22646)
                     /locus_tag="ACHBJ1_001060"
     CDS             complement(22176..22646)
                     /locus_tag="ACHBJ1_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM9430021.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(22694..23608)
                     /locus_tag="ACHBJ1_001061"
     CDS             complement(22694..23608)
                     /locus_tag="ACHBJ1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM9430022.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            23754..24557
                     /locus_tag="ACHBJ1_001062"
     CDS             23754..24557
                     /locus_tag="ACHBJ1_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM9430023.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            24735..25682
                     /locus_tag="ACHBJ1_001063"
     CDS             24735..25682
                     /locus_tag="ACHBJ1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM9430024.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(25750..26043)
                     /locus_tag="ACHBJ1_001064"
     CDS             complement(25750..26043)
                     /locus_tag="ACHBJ1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM9430025.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(26071..26736)
                     /locus_tag="ACHBJ1_001065"
     CDS             complement(26071..26736)
                     /locus_tag="ACHBJ1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM9430026.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(26876..27508)
                     /locus_tag="ACHBJ1_001066"
     CDS             complement(26876..27508)
                     /locus_tag="ACHBJ1_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9430027.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(27515..28582)
                     /gene="liaS"
                     /locus_tag="ACHBJ1_001067"
     CDS             complement(27515..28582)
                     /gene="liaS"
                     /locus_tag="ACHBJ1_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM9430028.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(28579..29310)
                     /gene="liaF"
                     /locus_tag="ACHBJ1_001068"
     CDS             complement(28579..29310)
                     /gene="liaF"
                     /locus_tag="ACHBJ1_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM9430029.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
BASE COUNT         8839 a         5984 c         4914 g         9733 t
ORIGIN      
        1 tcatgataaa caaccatgtc ttgataaaat tcgatcatag cttcgccgaa atgtgtagat
       61 gaaatatcat aaagtttagc ttctaaaact tttggatctt taggaacttc actggaaata
      121 taatcaataa gtgctggtgc aaagtcttcg tcggtttcaa aagttttccc taatgaagga
      181 tgatcgaaca atcggttcaa ataagcattt ccttccacga cacaaggtgt tccggctgcc
      241 atcgcttctg tataagtaag cccttgtgtt tctgaagtag aagcactgac aaaataatct
      301 gccgctttat aataaagtgc tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat
      361 tcttctaact gcagttctga gatcaattcc tctaactctt ctacataagg tccttttcct
      421 acgataacta atctggcgtt aggataagcg gagatgacct gaggaaaccc attaatcaag
      481 gcttggatat ttttctcata agaaatcctg cttaaagaaa gaatcatcaa atggttttcc
      541 tgaagaccta gtgactccct taatttgctg atatcctgct gagtgatatc agaacgtttg
      601 aatttttcaa tgtcgatacc agtaggaatc acgcgaagcg gtgctattac accatagctg
      661 cgtaatgtat caataacacg ttcacttgga caaactacac ctgtcgtatg atttgcaaat
      721 aatctagaca agtattttac atgggattgc ctgatcactt ttcctttacc gatataatga
      781 aggtagtctt catacatcgt atggtaagta tggatcagag gtattttcaa cttcttagct
      841 accatcttcc caagtatccc tgctccgaat tctgtatggg tatgaataag atctagttct
      901 agttctttgg caaccaaata agcataccac atgcctctaa caacaactct tcgatccttg
      961 aaagaaataa atggtacact tggcattcga atcacatctt tttccaactt atccgcatta
     1021 ggatcggtcg ttgtaaaaat atagacttca tgcccatgtc tttctaattc ttgttttaat
     1081 gttttgatcg atgtagcaac tccgctgact tgcggaaagt aggtatcggt aaaaaagccg
     1141 attttcatgt ttttcgcctc caaactagtc cttgacctga tttacttttt ttaatttgct
     1201 agagactttt tcattcagta cactttcgta tacattttta agttcttcac cgattttatt
     1261 gatacttcgc tcttttgctg tttgataacc ggcttgtgtc gtactcggta gttcttgatt
     1321 ggtaatctgt tggatcagtc ggataaattc ttcgtttgtc ttccccatat agcagttctt
     1381 gctatcttct aaccagccat tatacactgg aatatctcga acaagtacgt tttgtttgct
     1441 tgctaaagct tctaaaacaa cgatcccttc tgtctcttca taagaaggga aaaagaatag
     1501 atctgcacct gaatacgcac cttctatgat ctctccacga atgtaaccag gaaacaggac
     1561 attttcagga tgatcttctt ttacaatttt ccggatgttc cgaggaatcg agtacatggg
     1621 aacatgacca aaccaaataa atgtgtattc gggcatcttc ttagcaattt ctacgaaatc
     1681 aatgattcct ttacgttcga aaaacaaacc gacacaaatg attacttttt gatccggaga
     1741 cagtttgaaa tattctctga acttttgttc tttcattgga tcagggtgat atttctctaa
     1801 gtcgatccca ttggaaatag cttgaatcgg cacacggata ccatagcttt ccaacaactg
     1861 ttttgaataa ggtgttggcg tgatcagata gtccgctttt tggtacaatc ctaccagata
     1921 tttttttacg ataggagaaa gctggttcga tctgataaag gaatttcgga aatcttcctc
     1981 tgtagaatgc gcatgataga caactttttt tccttctctt ctggctttat tgaccatatt
     2041 atgactattg attccgtatg tattgatgtg caggatgtca taatcttctt tttcatctag
     2101 tgtataagag ataccgactt ccgtcagagc acgtttttga tgatcaagcg cacgcccgat
     2161 tccagatttt gcaagcatct tttcactttc aaaatataat aataccttca ccttacgtcc
     2221 cccttcactt atatagtata acatagccta caaactgtca ctcaaactta agccctattt
     2281 ttgtctattc ttactgacaa aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct
     2341 cttttacaag ccacaccagc ttttgatacg ttgtttattt tacatattca tgaacaagtt
     2401 caacgacttc ttccatcata tcgcattctt tcaatgcacg atcagcaagt tcagccattt
     2461 tcgctgtgtc caagcgtttc attaaactac gcgttttaag aatagatgtc gcactcattg
     2521 agaactcatc tagacccata cctactagca atggaacagc tgtttgatct ccagccattt
     2581 ctccacacat acctgcccat tttccttcag cgtgtgctgc atcaattaca tttttgatca
     2641 aacgtaaaat tgatgggtta tatggttggt acaagtaaga aacgcgttcg ttcatacggt
     2701 ctgctgccat tgtgtattga atcaaatcgt tcgtacctac agagaagaaa tctacttctt
     2761 ttgcaaactt gtctgctaag actgctgctg ctggaatctc gatcatgatg ccgacttgga
     2821 tcgtatcaga tacttccttg ccttcactta tcaatttttg tttttcttct tcaaagatcg
     2881 cttttgctgc gcggaattct ttgagtgtag ctaccatagg gaacatgata cgtaaattac
     2941 catgaacaga tgcacgaagc aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt
     3001 cagataaact gatacgcaat gcacgatatc ctaagaatgg attcatttca tgaggtaatt
     3061 gcagatatgg tagttcttta tctccaccga tgtccattgt acgaacaact acaggttttc
     3121 cttccatacc ttctaataca gctttgtaag cttcatattg gtcatcttct gttgggaagt
     3181 ctggagaatc catatacagg aattcagtac ggtacagacc aactgcttct ccaccattat
     3241 tatggacacc aactagatct tttggtgttc cgatgtttgc agctaattca aaatgcttgc
     3301 cgtcagctgt gacagtttct gcatttttta atttttccca ttctgctttt tgtgctgcgt
     3361 aatctgcacc tgctttttca aattcagctt tttgttcatc tgtaggatcg atgattacat
     3421 cgccttcgat cccgttgaca gccaaaatat cgccttcttt gactttagca gtaatttctt
     3481 ttgtcccaac gattgctgga atttctaaag aacgtgccat gatagcagaa tgtgaagtac
     3541 gtccaccaat gtctgttaca aacgctttta caaaattgcg atctaattgt gctgtatcac
     3601 ttggagtcaa gtcatgtgca acaacgacca cttcttcgtt gatcattgat ggatttggta
     3661 aagtaacgcc taataagtgc gctaaaatac gtttagcaac gtcacgaata tctgcagcac
     3721 gctcttgcat ataagcatta tcttccattg cttcgaacat accgatatac atatccgtta
     3781 cttcttttag cgctgattca gcatttacgc tgttgtcttt aatattttgc ttgatttgac
     3841 ctaccatttc tggatctgaa agaaccatca aatgggcatc aaatacttgc gcttctgctt
     3901 cacctaagct ttgcgctgct ttttcacgaa tttgctgcaa ttcttcagta gatttagcta
     3961 atgcagcatc caaacgagct tcttcagccg cagtatcttc tactgttgcc ttactgaatg
     4021 ttaaatccgg ttgaactagc aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa
     4081 tcccttttag catttcaacc attattcaga taagccttct ttcttcattg tttcaacgat
     4141 tgctgccatt gcatcagctt cgtcagcacc ttctgcagta atagtaacat cagaaccttg
     4201 acctacacct aaagacataa cgcccatgat tgatttcaag tttactgatt tacctttata
     4261 ctctaagttt acatcagagt taaatttgct tgctgtttgt actaatagtg tagctggacg
     4321 agcgtggatc ccagtttctg ctactacgtg aaattctttc ttttccatat taatcggtct
     4381 cctttaagtg agtgttgttt ttatgtgagg gttttctttt ttagtaattc agagaatcct
     4441 aacaaatgcc accctttcat ctgtaaagat taacattttt tttcgttaga tacaacagtt
     4501 ttcattcaat ttcagataaa agtattcaaa ttattccgga ttctttttca tccttttctt
     4561 cacattttat aacaaacgtg tttatttcct ttttcttgtc gctttctctt ctcctgacgt
     4621 ttatcattct ttcctttgac cctctttgtt ttcatatgaa acgaattact tgcttccctt
     4681 tttattttta gtataataag agtcaaaggt caggaatggt caatagagat ttcacctttt
     4741 ctacctcata aatctcttaa aaaactaggg gttattcttt tacctcatga aaaacgagat
     4801 gtaaaatatg gatgtaccca tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa
     4861 ctgcggcaag aatgaggcaa cgattcactt gtatgccact gtcaatggtc agcggacaca
     4921 actcgattac tgtcagagct gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac
     4981 taatatggcc caaaatgatc catttggatt tggcagtttg gacgatcttt tccgttccat
     5041 gtctcgccaa atgcaacagc aaggaaatta tgagcaaaca ccaccaacac aatttggcgg
     5101 aaacaacaac ttcaatggtg gtcagccacc tcaaggcaat acagaaggtt tattgggaga
     5161 atacggaatc aatatcacag aacaagcacg acaaggtgat atcgatcctg tgatcggacg
     5221 cgatgacgaa atcaaacgag tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt
     5281 cttgattgga gaaccaggtg ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat
     5341 cgtagatggt gatgtaccgc aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt
     5401 atctttagtc caaggtactg gtatacgagg acaatttgaa gaacgtatgc aaaaattgat
     5461 tgaagagatc cgtcaagcag aaaatgtgat cctattcata gatgaagttc acgaaatcgt
     5521 cggtgctggt tctgctggtg atggaaatat ggatgctggt aatattttaa aaccagcttt
     5581 agctcgagga gaattgcaaa tggttggtgc tactacctta aatgagtacc gcatcatcga
     5641 aaaagacgct gctttagaac gtcgtatgca acctgtacgg gtagatgaac caactgtcga
     5701 agaaacgatc agtattttga aaggtttgca aaaacgttat gaagactatc atcatgtaaa
     5761 atatactgat gaggccatcg aagcagccgc aactttatcc aatcgttacg tccaagatcg
     5821 cttcttgccg gataaagcca tcgatttgct agatgaaaca ggttctaaga aaaacttgac
     5881 gatccaaatc gttgatccta aaacaatcga gaaaaaactg caagaagcag aagaacaaaa
     5941 agttttagct tctcgagaag aagatttcga aaaagcagct tattatcgcg atcagatcaa
     6001 taaacttcaa aaaatgaaag aacgtcagct cactgaagag gaaacacctg tgattaccga
     6061 aaaagatatg gaaaaaatcg ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga
     6121 aaaagaacag acacaactga aaaacttagc agatgacttg aaagctcatg tcatcggaca
     6181 agataacgct gtagatcgag tagcgaaagc gattcgccga aaccgtgttg gattaaacaa
     6241 acagaatcgt ccaatcggct ccttcttatt tgttggacca actggtgtag gtaaaacaga
     6301 gcttgcgaaa caacttgcct acgaactatt cggttcacaa gattcaatga ttcgtttcga
     6361 tatgtctgaa tatatggaaa aacatagtgt ctctaaattg attggttctc ctccaggtta
     6421 tgtcggatac gaagaagctg gtcaattaac agaaaaagta cggagaaatc cttacagtct
     6481 tgtcttgtta gatgaagtag aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat
     6541 cttagatgat ggtcgattga cagatgcaca aggaagaacg gtcagcttta aagatacgat
     6601 catcattatg acaagtaatg ccggaactgg taaagtagaa gcaaacgttg gttttggtgc
     6661 ggcacgcgaa ggtgttactc gttctgtatt gaaccaatta aacaactact ttacaccaga
     6721 atttctgaac cgttttgacg ggatcatcga attcagtgca ttatcaaaag aaaacttgat
     6781 gacaatcgtc acactaatgc tagacgatgt caaccaaatg ctggcagcac agcaacttca
     6841 catcgaagtt cctacgaatg tcaaagaaaa attagttgac ttaggctatg atccatctat
     6901 gggtgctcgt cctcttcgac gcacgatcca agaacaaatc gaagatggta tcgctgaatt
     6961 ttatcttgat cacccttcta tccatgagtt gaaagcaaaa ctggataaag atggaaaaat
     7021 catcgttact tctaaaccag aacgtttggc taaagaatca gccgaagaaa cggctgaata
     7081 aatccagttt tatagcgtga gacggtattt ctttgagaaa tgccgtctca ttcttttttc
     7141 agaaaagtca aaatactgtt cattttataa aaaagaaatt ataataagag aaacaagcaa
     7201 ttaaaaatac ataaaagagg tgagcgccat gaaattggta aatgtaacaa acagccattc
     7261 ccgtcttgta ttaaaccagc ttgaaaacac tgatgcccat ctagtcaagg tctacacagc
     7321 cggaaatacc actattgtct acactgaagc ccctgaacat aatgaaattc tgctgatcaa
     7381 tgacaaacgt aaaatccagc cgaaagaaat cgaagatgct aaaaattatt tcttagataa
     7441 aatcaaagac gccgtttacc acgaggatga aatcaaagta atcgaattgg caggccttgt
     7501 tgaaatctcg attccaaaac atgaattagg cgctacatta gccgtgtaaa caaattttga
     7561 acataataat taaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     7621 acgtataaac ggcgaaagca gaagcaactc cttcggaaat aagctgaaat tcacgaaaat
     7681 tgcttttcaa atttttgtga attccagctt atttcttgga gttaaacgct tctgtctcag
     7741 cctctttttc tttctagctt tttgattttt ctcttttctt ttgtactttt ctcttttcat
     7801 tttcagttct acgcgtgtct ttatttttaa ctttttcctc tttatggatg attgatcgta
     7861 ctttttgtgc aatttgctcg tttttgtccc agtcttttac atctaataat cgacataatc
     7921 gtaacatctc ttcttttttc tgtttttttc tttcacgtct ttgccattct gtttccatgt
     7981 gtcacactct cctttcttta atcatatgta cgcaaaataa tttttatatt tttttgatta
     8041 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
     8101 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
     8161 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
     8221 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
     8281 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
     8341 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
     8401 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
     8461 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
     8521 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
     8581 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
     8641 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
     8701 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
     8761 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
     8821 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
     8881 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
     8941 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
     9001 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     9061 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     9121 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     9181 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     9241 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     9301 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     9361 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     9421 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     9481 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     9541 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     9601 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     9661 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     9721 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     9781 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     9841 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     9901 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     9961 tgctcttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
    10021 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
    10081 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
    10141 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
    10201 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
    10261 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
    10321 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
    10381 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
    10441 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
    10501 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
    10561 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
    10621 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
    10681 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
    10741 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
    10801 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
    10861 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
    10921 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
    10981 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
    11041 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
    11101 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
    11161 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
    11221 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
    11281 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
    11341 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
    11401 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
    11461 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
    11521 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
    11581 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
    11641 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
    11701 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
    11761 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
    11821 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
    11881 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
    11941 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
    12001 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
    12061 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
    12121 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
    12181 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
    12241 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
    12301 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
    12361 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
    12421 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
    12481 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
    12541 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
    12601 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
    12661 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
    12721 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
    12781 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
    12841 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
    12901 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
    12961 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
    13021 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
    13081 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
    13141 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
    13201 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
    13261 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
    13321 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
    13381 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
    13441 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
    13501 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
    13561 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
    13621 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
    13681 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
    13741 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
    13801 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
    13861 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
    13921 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
    13981 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
    14041 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
    14101 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
    14161 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
    14221 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
    14281 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
    14341 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
    14401 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
    14461 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
    14521 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
    14581 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
    14641 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
    14701 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
    14761 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
    14821 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
    14881 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
    14941 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
    15001 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
    15061 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
    15121 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
    15181 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
    15241 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
    15301 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
    15361 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
    15421 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
    15481 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
    15541 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
    15601 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
    15661 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
    15721 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
    15781 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
    15841 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
    15901 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
    15961 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
    16021 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
    16081 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
    16141 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
    16201 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
    16261 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
    16321 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
    16381 ggtggctgtc aattacaaca cttagcttat caagctcaat tggattttaa aaaggatctg
    16441 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
    16501 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
    16561 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
    16621 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
    16681 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
    16741 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
    16801 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
    16861 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
    16921 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
    16981 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
    17041 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
    17101 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
    17161 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
    17221 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
    17281 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
    17341 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
    17401 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
    17461 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
    17521 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
    17581 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
    17641 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
    17701 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
    17761 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
    17821 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
    17881 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
    17941 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
    18001 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    18061 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    18121 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    18181 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    18241 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    18301 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    18361 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    18421 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    18481 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    18541 ttgctgtgag ccttcatggt aaactaatat tatagaaagg agttagtaaa atgaagatga
    18601 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    18661 ggttgcttag cggaaatccc ggaaaatatt actattcttt gcaacttcca ccatttgcac
    18721 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    18781 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    18841 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    18901 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    18961 tgtggctgca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    19021 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    19081 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    19141 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    19201 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    19261 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    19321 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    19381 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    19441 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    19501 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    19561 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt
    19621 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    19681 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    19741 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    19801 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    19861 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    19921 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    19981 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcgtaggca tgatttaatt
    20041 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    20101 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    20161 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    20221 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    20281 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    20341 tcagcaaaac gaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    20401 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    20461 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattatta ttttttaaaa
    20521 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    20581 gcagttcgca atcgttttat ctttttttat gtattttttc cagaaaaagc gaacaacaag
    20641 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    20701 gtctttgcct accgttcaga ttatcattcg atttcacgac aatttcttcc tatgactggt
    20761 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    20821 tgactcgaac tagtgattgc attaccatca tcttttaatg gatagatatg ctcctcttca
    20881 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    20941 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    21001 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    21061 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    21121 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    21181 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    21241 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    21301 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatatc
    21361 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    21421 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    21481 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    21541 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    21601 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    21661 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    21721 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    21781 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    21841 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    21901 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    21961 tcaattgctg aatttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    22021 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    22081 gtgtaagtca taaaaaaatt cctcctcaat gttttcttaa taatgtctac attacaaaga
    22141 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    22201 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    22261 gcaaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    22321 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    22381 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    22441 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    22501 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    22561 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    22621 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    22681 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    22741 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    22801 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    22861 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacatcct
    22921 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcgata gcaggtgcac
    22981 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    23041 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    23101 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    23161 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    23221 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    23281 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    23341 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    23401 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    23461 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    23521 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    23581 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    23641 taacattttt ttgatatttc accaatcatt cagctgtttc cttaaagcac ttgtatttca
    23701 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    23761 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag gcgagaaatg
    23821 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    23881 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    23941 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    24001 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    24061 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    24121 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    24181 aacttcccta caccaatgaa tctttaaaaa acttaacagg taagcagctc cgttcttttt
    24241 taagtgaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    24301 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    24361 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    24421 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacta
    24481 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    24541 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    24601 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    24661 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    24721 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    24781 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    24841 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    24901 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    24961 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    25021 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    25081 aaaaggcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    25141 ggaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    25201 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    25261 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    25321 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    25381 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    25441 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    25501 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    25561 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    25621 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    25681 aatagctgaa aacaatattt tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc
    25741 tctctttttt tattgttttt taaagtgata ggttggctcc tccaggttgg gattataatc
    25801 aacaagtaaa tcatatcctt taaagaacca ttcatctgct tcctcgataa aaaaaagtgt
    25861 cccatcaatt tccactttct ttacaggatg ttctggctgt tcaactgaca ttccaatcga
    25921 aaaaccatca tgaacctctg ttttgccgta gacttttcca aaaaaacgta ttccgtaagt
    25981 ttctggcaga tcaatttctg agtcaaacca cttttgtgct tttggcgtta ccgttaattt
    26041 catcccaaca acctactttc tcttttcttt ctattgattc atcttatcgt ctagaatatc
    26101 cacatcgcct gtctctccaa taaccagtag attatcattt tcacgaacat attcatctgc
    26161 tgcaggagaa gcgataaccg tttgatctac tcttcgtatg gcgactactg ttaagtcaaa
    26221 acgttgcctg aaatttaatt cagctaatgt tttgttgaaa aatttaggat tggagactcg
    26281 gatttctgct aaagaatatt tatctgacaa ctctaggtaa tctaaaatat tctttgacac
    26341 taaattatgc gcaatcctta ttcccatgtc tcgctcggga tggacgacgc gatcagctcc
    26401 gatcttttca agtactctgg catgatactc attttgggct tttgctaaaa tatttggtac
    26461 gcccatttcc tttaccatca gagtaactaa gatactggct tgaatatctt ctccaatagc
    26521 gacaattaca tgatcgaaat ttcttatgcc taatgatcga agtgtcattt catcctgtgc
    26581 atttgccaca acagcatgag tggctatatt catatattca ttgacccgat cttcattact
    26641 atcaatagcc agtacttctt gaccagcttc gatcagtgtt cggcagatac ttccgccaaa
    26701 tcgtccaaga cctacaattg caaaattttg tttcaccgtt ttcctccttt agtatcttct
    26761 tttctcagta taacaaagcc tttacaaaaa caaaagcaag catcattatt gtaaaaaagc
    26821 caagaaaaat aatagttatt ttctcagctt ttgattatcc tctttatcat cgtttttatt
    26881 tcaccaaacc atgtttgaat gcatagatag cggcttgtgt acggtcttcc acttctagct
    26941 tagccaatat attggaaaca tgtgttttta ctgttttcag tgtaataaac aattcgtccg
    27001 ctatttcctg attacttttt ccttcagaaa tcagcattaa aatttcattt tcccgattcg
    27061 ttagttcttc atgtaataca ggctcatttc gccgactcat tttttccatc atttttgttg
    27121 tcacttctgg ttctaatact cgctcgccac gttgcgttgc ccttatagca tcagcaattt
    27181 catgtgcggt agaggtcttc aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa
    27241 ctttttcgtc atcaataaaa ctggttacga taatgatttt agcttctggc caatctttta
    27301 agattgcttt tgttgattcg ataccatcca tttcttccat gactaagtcc attaaaatga
    27361 cgtccggtcg aagtgtcatc gccttctcat agccttgtcg tccattttca gcttctccaa
    27421 ttacttcaat gtcttcttga atagataaat aaaaagagac gcctaagcgc accatttcat
    27481 ggtcatctac taataaaact tttatcatcc ttatctatgc ctccttcatc aaagggacct
    27541 tgatttctac acttgtacct tgacctttaa aactaatgat ttttactgtt ccaccaatac
    27601 cagctactcg ttctttgata ttgttcaatc cataacttcc cgttttcgtt tcattcatat
    27661 taaaccctgt tccgtcgtca ataatacgta acaaaagatt attgtctatt ttgtgcaaat
    27721 atacctctaa ttcattggct ttagcatgtc ttaatgtatt tgataacaat tcttgaacga
    27781 ttcggaacaa gtgatcctca atggaactag atagttttac atcttctaca tcccatttca
    27841 gcgaaatctg gattttattt tgcaattcct tcaataactg ctcgatacct tgctttaaac
    27901 ttttctcttc taaattgact ggacgtagat gaagaagcag cgcacgcatc tcagactggg
    27961 aagcgttgat gatctctgct accattttca actgcttgcg aaacatttct ggtgtctcgc
    28021 ttttttctgc ttgctccgtc aatgccgaca tcatcatcat tgctgcaaac aattgttgcg
    28081 aaactgaatc atgcagctca cgggctaatc gatgtctttc taattctaaa atttcttctt
    28141 tggtttgtcc atctacatat tggggacggc tcgttacaat ttgtaattca ctagatactt
    28201 caatcatttt ttcttttatc ttagtgattt ccttatcaat atctttgatg tataaatcgt
    28261 ccgatgcact tgggatcgga agaagcaaca atttggattc ataattgcca gaagaaagtg
    28321 cacgcagctt ttcttcgatt ttcccgtatt ttgtcttttg aataagtgat aataatagaa
    28381 agacaattaa tcctacacct aaggaaatgg ctaacaaatg gaagataagc ggaacatata
    28441 ataaacgtgc acgcagtaat tctccccacc aatgactttg attgctggca taaaaataag
    28501 tgaaaagtga aaatagaata ataaggaaag ccgcaatccc cgaataaaca gctagcatcg
    28561 ctctggatat ttttcccatc atattcgtat cacctcgaca tctccaatca aggtattagt
    28621 aataatcttc aatctacgag ggttttcatc ataatcactg ctgtagattt tgatttgttc
    28681 atttttcaaa gaaaattgct cttcttcaaa caagacattt cctactaatg tcgcatgctc
    28741 taatcggatg gctacgccca aaggtactaa gatccgtgtt ctgcctatcc cttttcttac
    28801 aatcacaata ttatcatctt ttggcaaaag cgtattgccc aaatctatga tagtatcacc
    28861 agaaatgacg gcgatgttga tatcgtccca ttcatagacg tcgtttccaa tacgttggtt
    28921 accgaataat tgctgtcttt tccgctcatt attatgtgtt tttaattgct ctgtttggat
    28981 catcatgatc tgtttctttc gccaaaaagc atttttggtt aaatctatcc ctgaaatctc
    29041 tacacctttc agaccaataa ataaaacggc aaatacagcc atcatccata aagcaggact
    29101 gttgactaag ctaaaaaaaa tgaccaaact tcctaatatc aattgaaaat tttgaaattt
    29161 agtacgagga tgtttgcgca tagctagata tatattgaaa ataccgaata tcactaaaag
    29221 caaaagtcca gtattgttta cgatttgcca aacagcaaat aacagcagta gtgcttcaac
    29281 taccacaaaa aaacgccaag aactattcat cgctctttct ccctcctgac acattctcat
    29341 ttcatcatac ctattttttt aactgttggc tataggactt tagatgtaag atcgtttgtt
    29401 gaaatctaaa atacacaaag agaaaaagat ctagagtgta tcatcagtaa gatgaactct
    29461 ctagatcttt
//
LOCUS       DAYYNQ010000027        29128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000027 DAYYNQ010000000
VERSION     DAYYNQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.027"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1031)
                     /locus_tag="ACHBJ1_001069"
     CDS             complement(<1..1031)
                     /locus_tag="ACHBJ1_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9430030.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="ACHBJ1_001070"
     CDS             complement(1113..1802)
                     /locus_tag="ACHBJ1_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9430031.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBJ1_001071"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="ACHBJ1_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM9430032.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBJ1_001072"
     CDS             complement(2329..2832)
                     /gene="rplJ"
                     /locus_tag="ACHBJ1_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM9430033.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBJ1_001073"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="ACHBJ1_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM9430034.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBJ1_001074"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="ACHBJ1_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM9430035.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHBJ1_001075"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="ACHBJ1_001075"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM9430036.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHBJ1_001076"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="ACHBJ1_001076"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM9430037.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7544)
                     /locus_tag="ACHBJ1_001077"
                     /pseudo
     CDS             complement(6444..7544)
                     /locus_tag="ACHBJ1_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7636..8523)
                     /locus_tag="ACHBJ1_001078"
     CDS             complement(7636..8523)
                     /locus_tag="ACHBJ1_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9430038.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHBJ1_001079"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="ACHBJ1_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM9430039.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHBJ1_001080"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="ACHBJ1_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM9430040.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_001081"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9430041.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHBJ1_001082"
     CDS             9754..10398
                     /gene="cbpA"
                     /locus_tag="ACHBJ1_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM9430042.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="ACHBJ1_001083"
     CDS             complement(10454..10957)
                     /locus_tag="ACHBJ1_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9430043.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="ACHBJ1_001084"
     CDS             11554..12519
                     /locus_tag="ACHBJ1_001084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9430044.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="ACHBJ1_001085"
     CDS             complement(12618..13790)
                     /locus_tag="ACHBJ1_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430045.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="ACHBJ1_001086"
     CDS             complement(13777..14922)
                     /locus_tag="ACHBJ1_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430046.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="ACHBJ1_001087"
     CDS             complement(14936..15868)
                     /locus_tag="ACHBJ1_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430047.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="ACHBJ1_001088"
     CDS             15985..17064
                     /locus_tag="ACHBJ1_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9430048.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="ACHBJ1_001089"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="ACHBJ1_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17789..18016)
                     /locus_tag="ACHBJ1_001090"
     CDS             complement(17789..18016)
                     /locus_tag="ACHBJ1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9430049.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="ACHBJ1_001091"
     CDS             complement(18119..20707)
                     /locus_tag="ACHBJ1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM9430050.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="ACHBJ1_001092"
     CDS             complement(20727..21386)
                     /locus_tag="ACHBJ1_001092"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9430051.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHBJ1_001093"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="ACHBJ1_001093"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM9430052.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="ACHBJ1_001094"
     CDS             complement(22159..23025)
                     /locus_tag="ACHBJ1_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM9430053.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="ACHBJ1_001095"
     CDS             23134..23775
                     /locus_tag="ACHBJ1_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM9430054.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHBJ1_001096"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="ACHBJ1_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM9430055.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="ACHBJ1_001097"
     CDS             complement(25216..26115)
                     /locus_tag="ACHBJ1_001097"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM9430056.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="ACHBJ1_001098"
     CDS             complement(26117..26914)
                     /locus_tag="ACHBJ1_001098"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM9430057.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="ACHBJ1_001099"
     CDS             complement(26892..27596)
                     /locus_tag="ACHBJ1_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM9430058.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="ACHBJ1_001100"
     CDS             27727..28302
                     /locus_tag="ACHBJ1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM9430059.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="ACHBJ1_001101"
     CDS             28420..29097
                     /locus_tag="ACHBJ1_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM9430060.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8572 a         6170 c         5237 g         9149 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaa
//
LOCUS       DAYYNQ010000028        28599 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000028 DAYYNQ010000000
VERSION     DAYYNQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.028"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHBJ1_001102"
     CDS             complement(<1..1835)
                     /locus_tag="ACHBJ1_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9430061.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHBJ1_001103"
     CDS             complement(1832..2536)
                     /locus_tag="ACHBJ1_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9430062.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHBJ1_001104"
     CDS             complement(2548..4857)
                     /locus_tag="ACHBJ1_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9430063.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHBJ1_001105"
     CDS             complement(5052..5516)
                     /locus_tag="ACHBJ1_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430064.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHBJ1_001106"
     CDS             complement(5516..6142)
                     /locus_tag="ACHBJ1_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9430065.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHBJ1_001107"
     CDS             complement(6149..6514)
                     /locus_tag="ACHBJ1_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9430066.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHBJ1_001108"
     CDS             complement(6504..6842)
                     /locus_tag="ACHBJ1_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430067.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHBJ1_001109"
     CDS             complement(6832..7158)
                     /locus_tag="ACHBJ1_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9430068.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHBJ1_001110"
     CDS             complement(7139..7417)
                     /locus_tag="ACHBJ1_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9430069.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHBJ1_001111"
     CDS             complement(7419..8783)
                     /locus_tag="ACHBJ1_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9430070.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHBJ1_001112"
     CDS             complement(8796..9365)
                     /locus_tag="ACHBJ1_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM9430071.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHBJ1_001113"
     CDS             complement(9337..10524)
                     /locus_tag="ACHBJ1_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9430072.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHBJ1_001114"
     CDS             complement(10588..12315)
                     /locus_tag="ACHBJ1_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM9430073.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHBJ1_001115"
     CDS             complement(12312..12764)
                     /locus_tag="ACHBJ1_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM9430074.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHBJ1_001116"
     CDS             complement(12876..13256)
                     /locus_tag="ACHBJ1_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM9430075.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHBJ1_001117"
     CDS             complement(13253..13639)
                     /locus_tag="ACHBJ1_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM9430076.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHBJ1_001118"
     CDS             complement(13641..13919)
                     /locus_tag="ACHBJ1_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430077.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHBJ1_001119"
     CDS             13967..14695
                     /locus_tag="ACHBJ1_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM9430078.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACHBJ1_001120"
     CDS             complement(14826..15302)
                     /locus_tag="ACHBJ1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM9430079.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACHBJ1_001121"
     CDS             complement(15262..15618)
                     /locus_tag="ACHBJ1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430080.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACHBJ1_001122"
     CDS             complement(15596..15781)
                     /locus_tag="ACHBJ1_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430081.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACHBJ1_001123"
     CDS             complement(15782..16054)
                     /locus_tag="ACHBJ1_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM9430082.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACHBJ1_001124"
     CDS             complement(16051..16251)
                     /locus_tag="ACHBJ1_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430083.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="ACHBJ1_001125"
     CDS             16379..16591
                     /locus_tag="ACHBJ1_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430084.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="ACHBJ1_001126"
     CDS             complement(16624..16818)
                     /locus_tag="ACHBJ1_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430085.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="ACHBJ1_001127"
     CDS             complement(16831..17013)
                     /locus_tag="ACHBJ1_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430086.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="ACHBJ1_001128"
     CDS             complement(17010..17150)
                     /locus_tag="ACHBJ1_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430087.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="ACHBJ1_001129"
     CDS             complement(17640..18005)
                     /locus_tag="ACHBJ1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430088.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="ACHBJ1_001130"
     CDS             complement(18045..18347)
                     /locus_tag="ACHBJ1_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9430089.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="ACHBJ1_001131"
     CDS             complement(18347..18646)
                     /locus_tag="ACHBJ1_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430090.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="ACHBJ1_001132"
     CDS             complement(18639..18803)
                     /locus_tag="ACHBJ1_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430091.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="ACHBJ1_001133"
     CDS             complement(18809..19078)
                     /locus_tag="ACHBJ1_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430092.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="ACHBJ1_001134"
     CDS             complement(19090..19839)
                     /locus_tag="ACHBJ1_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430093.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="ACHBJ1_001135"
     CDS             complement(19836..20528)
                     /locus_tag="ACHBJ1_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM9430094.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="ACHBJ1_001136"
     CDS             complement(20534..21205)
                     /locus_tag="ACHBJ1_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM9430095.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="ACHBJ1_001137"
     CDS             complement(21198..21539)
                     /locus_tag="ACHBJ1_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430096.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="ACHBJ1_001138"
     CDS             complement(21717..22415)
                     /locus_tag="ACHBJ1_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM9430097.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="ACHBJ1_001139"
     CDS             22502..22972
                     /locus_tag="ACHBJ1_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430098.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="ACHBJ1_001140"
     CDS             complement(23015..23194)
                     /locus_tag="ACHBJ1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430099.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="ACHBJ1_001141"
     CDS             complement(23181..23519)
                     /locus_tag="ACHBJ1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM9430100.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="ACHBJ1_001142"
     CDS             complement(23535..23738)
                     /locus_tag="ACHBJ1_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430101.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="ACHBJ1_001143"
     CDS             complement(23753..24529)
                     /locus_tag="ACHBJ1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM9430102.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24828..25148
                     /locus_tag="ACHBJ1_001144"
     CDS             24828..25148
                     /locus_tag="ACHBJ1_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430103.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25166..25597
                     /locus_tag="ACHBJ1_001145"
     CDS             25166..25597
                     /locus_tag="ACHBJ1_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9430104.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            25727..26188
                     /locus_tag="ACHBJ1_001146"
     CDS             25727..26188
                     /locus_tag="ACHBJ1_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFM9430105.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            26222..26857
                     /locus_tag="ACHBJ1_001147"
     CDS             26222..26857
                     /locus_tag="ACHBJ1_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM9430106.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            26970..27605
                     /locus_tag="ACHBJ1_001148"
     CDS             26970..27605
                     /locus_tag="ACHBJ1_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430107.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            27904..>28599
                     /locus_tag="ACHBJ1_001149"
     CDS             27904..>28599
                     /locus_tag="ACHBJ1_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFM9430108.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNY"
BASE COUNT         8376 a         5730 c         4478 g        10015 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    24781 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    24841 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    24901 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    24961 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    25021 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    25081 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    25141 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    25201 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    25261 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    25321 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    25381 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    25441 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    25501 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    25561 taagaaaaaa tattcttacg caaggcaata tgtatgaata catcaagaat tattaaaaaa
    25621 accgatttca gcaaaacatt aaaaaagccc gtgctgcaac acgggctctt tcctcatata
    25681 tgagcttcta caaaaaaaat catatcacag aaatgaggaa gaaaaaatgg aaaaagagat
    25741 tttaataaaa tctccaggaa aaaccttgat taaagtaact gatgattcta tttcaattat
    25801 tagaaaagga tttataaatt tagttaatca aggtattaaa ggagaaaaaa ctatcccctt
    25861 taaaaatata tcagccgtac aattaaaaaa accaggtatg agtaatggtt atatacaatt
    25921 taccttgcta ggcggaaacg aaagtcgcgg aggtatatta gcggccacga aagatgaaaa
    25981 caccgttatg tttacaaaaa aatattggaa tgaaatggaa aatttaaaaa aatatataga
    26041 gaagcaacaa tctatattag ataataattc caaagaaaca caaatttcaa gtgcagatga
    26101 aattaaaaag tataaagagt tgttagatga agggattatt aatcaagaag aattcgattt
    26161 taagaaaaaa gaattgcttg gtttataata ttcaatatca aaagagaaag aggatctatt
    26221 aatggacggt atatttggtt ttactggtat tgttttattt attataggtt taatcatgtt
    26281 aattgtaaga tttatcaaaa aaacaaataa gaaaacacct atgattctcc ttattatcgg
    26341 gattattttt actaccgtag gtttctcgct ttcctcatca aacgaggcaa atgacaataa
    26401 caaacaggaa agctcatcat ctgatactca aactactaca tctaacagca gcaataaaag
    26461 cctaactcaa tttgaagaat ttttagagac aaataatcat gactggggtg ctttcttaga
    26521 tagttattat tctattactc cagctactga gcaaaataca gcttttacta aatatattag
    26581 tggtaaaaca tatacttttg aaggaactgt tatcgaatca atgactacta gaatagccat
    26641 tatagcagac aaagagtacg atgataaaag ttggaatgat atttctgcaa cacctaaagt
    26701 ttcatatgta atttttgcga aggacttaaa caatgctgat acctttacaa agggcgataa
    26761 agtaactttt acaggagaaa ttagttcaag aggatcgaac atagaaaaat cctatgctca
    26821 atgggatatg attaatagta aagtaagtaa aaaataaatg aaccacgccc cactaagagt
    26881 tagattgtgc acaataataa aacctataca aaagagtgcc atcaggagtc acaatgcttg
    26941 taaaggaaat gacaaaatag gaggtaacta tggataatag caatgaaaaa ctaaccaaaa
    27001 aagaatatct tgaattaata gttgaaggcg gtattggagc aattccttat attggtggcg
    27061 ttgtgcagtc actttatttt gggtctaaaa acgagaagcg tttcaaacgc atcgagaaat
    27121 tctacaaaaa tttaaacgaa tcattagaaa acattcgcga tcaaattcca gaagatgttt
    27181 tcaacttaga gaacaaggac caactcattg gaatattcga agggataaat gacgaggtag
    27241 aaaaatcaaa ggcacaaaat aaaatagata tgtataaaaa tctttataag aattgtttac
    27301 ttcgaattaa caacgcttca tgggataacg aggagtatta tcttcttgta ctgaatcaat
    27361 taacatcgat tgagattcaa ttgttagcat gcttaatgag tcgtggaaac gaaaatttca
    27421 ctggaaatat cagcatgtct ggctattcac aagagctaat agatggtagc ttaaatagat
    27481 tatctgattt tggtctgtta gaaaaaacga ttagctccat agtattaggt gatgtcggca
    27541 aacaaaatat gggctacaaa atatctcatt taggaattca atttattaac tcaactctta
    27601 cttaagtttt ctttctaata tgacatctag acctatcagc aatgcaacaa taaaaaacca
    27661 gacataccaa taatcaacaa ataatctaaa ctctggaagt tcagttattc ttttaaaaat
    27721 cactttaatc ataataaaca cctctgattg gatgatctaa tacacatcca attataccat
    27781 taaaacgaca aatagcacat ccttgcgccg gcaagcaaaa aggatgtgca aaaaataata
    27841 aaacccaaac aatgggcttc tttatagttc ctattgtatc agagaaagag agctgattca
    27901 attatggcaa gattagtcaa acgtggaaat agttggcaat acgaaatttc atacaaaaaa
    27961 gatgacggaa aatacacgaa gataagaaaa tcaggattta agacaaaagg agaagcaaaa
    28021 gatgccgcca acgaattaga atataacttg aacaaaggcc ttaaagggga tcgcaaaaat
    28081 ctattattat cagattactt tgaggattgg atgcaacttt ataaagaagg aacagtatct
    28141 cctatcactt atagaaaata cgaagatacg ttaatgaaca taaagaaata tatgccagcg
    28201 gtattgattt ctgatttaga tagagtggga tatcaacgct ttttaaataa gtatgctaaa
    28261 gaccatgtaa aatctaccgt tattaagttt aataaccata ttagagcgtc gttgaaagat
    28321 gccgtagaag aaggattaat tccgtttgat ccaactagaa aagcagtaat caaaggaaaa
    28381 gattcattga agccaaaaga agataaatat ttagattatg atcaatttaa atctttaatg
    28441 aaactcgtag aagaaaacct ttctgcgcag tactcttctc ctatgctcgt gttagttgct
    28501 ggtgccactg gaatgcgatt tgctgaactt ctaggattaa catgggaaga tatcgatttc
    28561 gaagatcaaa tcatcactat taataaaaca tggaattat
//
LOCUS       DAYYNQ010000029        28554 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000029 DAYYNQ010000000
VERSION     DAYYNQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.029"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHBJ1_001150"
     CDS             complement(27..1259)
                     /locus_tag="ACHBJ1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM9430109.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHBJ1_001151"
     CDS             1514..2083
                     /locus_tag="ACHBJ1_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430110.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHBJ1_001152"
     CDS             complement(2261..2701)
                     /locus_tag="ACHBJ1_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM9430111.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHBJ1_001153"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHBJ1_001153"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM9430112.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHBJ1_001154"
     CDS             3655..4578
                     /locus_tag="ACHBJ1_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM9430113.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHBJ1_001155"
     CDS             complement(4803..5042)
                     /locus_tag="ACHBJ1_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430114.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHBJ1_001156"
     CDS             complement(5047..5466)
                     /locus_tag="ACHBJ1_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9430115.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHBJ1_001157"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHBJ1_001157"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9430116.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACHBJ1_001158"
     CDS             complement(7038..7673)
                     /locus_tag="ACHBJ1_001158"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430117.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACHBJ1_001159"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACHBJ1_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACHBJ1_001160"
     CDS             complement(8015..8650)
                     /locus_tag="ACHBJ1_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430118.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACHBJ1_001161"
     CDS             9162..10304
                     /locus_tag="ACHBJ1_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9430119.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACHBJ1_001162"
     CDS             10297..10503
                     /locus_tag="ACHBJ1_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9430120.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACHBJ1_001163"
     CDS             10582..11775
                     /locus_tag="ACHBJ1_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430121.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACHBJ1_001164"
     CDS             11980..13119
                     /locus_tag="ACHBJ1_001164"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM9430122.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACHBJ1_001165"
     CDS             13112..13912
                     /locus_tag="ACHBJ1_001165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9430123.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACHBJ1_001166"
     CDS             13912..14739
                     /locus_tag="ACHBJ1_001166"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9430124.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACHBJ1_001167"
     CDS             14744..15451
                     /locus_tag="ACHBJ1_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9430125.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHBJ1_001168"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHBJ1_001168"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM9430126.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHBJ1_001169"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHBJ1_001169"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM9430127.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHBJ1_001170"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHBJ1_001170"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM9430128.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHBJ1_001171"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHBJ1_001171"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM9430129.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACHBJ1_001172"
     CDS             19036..21069
                     /locus_tag="ACHBJ1_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430130.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACHBJ1_001173"
     CDS             21113..22393
                     /locus_tag="ACHBJ1_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM9430131.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACHBJ1_001174"
     CDS             22603..23409
                     /locus_tag="ACHBJ1_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430132.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACHBJ1_001175"
     CDS             23421..24641
                     /locus_tag="ACHBJ1_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430133.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACHBJ1_001176"
     CDS             24631..26217
                     /locus_tag="ACHBJ1_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430134.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACHBJ1_001177"
     CDS             26256..28394
                     /locus_tag="ACHBJ1_001177"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9430135.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         9144 a         4997 c         5745 g         8668 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgc
//
LOCUS       DAYYNQ010000030        27445 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000030 DAYYNQ010000000
VERSION     DAYYNQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.030"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBJ1_001178"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACHBJ1_001178"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9430136.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACHBJ1_001179"
     CDS             complement(937..1518)
                     /locus_tag="ACHBJ1_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9430137.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBJ1_001180"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACHBJ1_001180"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM9430138.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBJ1_001181"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACHBJ1_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM9430139.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBJ1_001182"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACHBJ1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM9430140.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBJ1_001183"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACHBJ1_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM9430141.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBJ1_001184"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACHBJ1_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM9430142.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBJ1_001185"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACHBJ1_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM9430143.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBJ1_001186"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACHBJ1_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM9430144.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBJ1_001187"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACHBJ1_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM9430145.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBJ1_001188"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACHBJ1_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM9430146.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBJ1_001189"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACHBJ1_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM9430147.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBJ1_001190"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACHBJ1_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM9430148.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBJ1_001191"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACHBJ1_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM9430149.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBJ1_001192"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACHBJ1_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM9430150.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBJ1_001193"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACHBJ1_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM9430151.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBJ1_001194"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACHBJ1_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM9430152.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBJ1_001195"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACHBJ1_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM9430153.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBJ1_001196"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACHBJ1_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM9430154.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBJ1_001197"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACHBJ1_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM9430155.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBJ1_001198"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACHBJ1_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM9430156.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACHBJ1_001199"
     CDS             13972..14157
                     /locus_tag="ACHBJ1_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM9430157.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBJ1_001200"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACHBJ1_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM9430158.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBJ1_001201"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACHBJ1_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM9430159.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBJ1_001202"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACHBJ1_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM9430160.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBJ1_001203"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACHBJ1_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM9430161.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBJ1_001204"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACHBJ1_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM9430162.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBJ1_001205"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACHBJ1_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM9430163.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACHBJ1_001206"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACHBJ1_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM9430164.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACHBJ1_001207"
     CDS             18232..18879
                     /locus_tag="ACHBJ1_001207"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM9430165.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACHBJ1_001208"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACHBJ1_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM9430166.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBJ1_001209"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACHBJ1_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM9430167.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBJ1_001210"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACHBJ1_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM9430168.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBJ1_001211"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACHBJ1_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM9430169.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACHBJ1_001212"
     CDS             20317..21255
                     /locus_tag="ACHBJ1_001212"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM9430170.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBJ1_001213"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACHBJ1_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM9430171.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACHBJ1_001214"
     CDS             21935..22774
                     /locus_tag="ACHBJ1_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9430172.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACHBJ1_001215"
     CDS             22750..23619
                     /locus_tag="ACHBJ1_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9430173.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACHBJ1_001216"
     CDS             23609..24406
                     /locus_tag="ACHBJ1_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9430174.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACHBJ1_001217"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACHBJ1_001217"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9430175.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26019..26213
                     /locus_tag="ACHBJ1_001218"
                     /pseudo
     CDS             26019..26213
                     /locus_tag="ACHBJ1_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="ACHBJ1_001219"
     CDS             complement(26401..27177)
                     /locus_tag="ACHBJ1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9430176.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            27300..>27445
                     /locus_tag="ACHBJ1_001220"
     CDS             27300..>27445
                     /locus_tag="ACHBJ1_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFM9430177.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVI"
BASE COUNT         8910 a         5038 c         5811 g         7686 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttttagtata attcaattcc atacaataaa
    27241 taaaccgaac aaaaaaagtc ttttactatt tttacttaat ttatgaaagg ttgtttacca
    27301 tgttagacca aacagatata aatatcattg aggaactatc aaaaaatagt cgaatcacaa
    27361 tgaaagaact tggagaaaaa gtacatttga ctggacccgc tgtctcggcg agagtgacaa
    27421 aactagagga aagtggagtt attga
//
LOCUS       DAYYNQ010000031        26974 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000031 DAYYNQ010000000
VERSION     DAYYNQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.031"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..258
                     /locus_tag="ACHBJ1_001221"
     CDS             <1..258
                     /locus_tag="ACHBJ1_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HFM9430178.1"
                     /translation="IRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFS
                     LAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            370..1821
                     /locus_tag="ACHBJ1_001222"
     CDS             370..1821
                     /locus_tag="ACHBJ1_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9430179.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2170..2802
                     /locus_tag="ACHBJ1_001223"
     CDS             2170..2802
                     /locus_tag="ACHBJ1_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM9430180.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3390..4829
                     /locus_tag="ACHBJ1_001224"
     CDS             3390..4829
                     /locus_tag="ACHBJ1_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9430181.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5124..5342
                     /locus_tag="ACHBJ1_001225"
                     /pseudo
     CDS             5124..5342
                     /locus_tag="ACHBJ1_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5591..5806
                     /locus_tag="ACHBJ1_001226"
     CDS             5591..5806
                     /locus_tag="ACHBJ1_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430182.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6023..6279)
                     /locus_tag="ACHBJ1_001227"
                     /pseudo
     CDS             complement(6023..6279)
                     /locus_tag="ACHBJ1_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6466..7377)
                     /locus_tag="ACHBJ1_001228"
     CDS             complement(6466..7377)
                     /locus_tag="ACHBJ1_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9430183.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7396..8400)
                     /locus_tag="ACHBJ1_001229"
     CDS             complement(7396..8400)
                     /locus_tag="ACHBJ1_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM9430184.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8397..10520)
                     /locus_tag="ACHBJ1_001230"
     CDS             complement(8397..10520)
                     /locus_tag="ACHBJ1_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM9430185.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10525..12972)
                     /locus_tag="ACHBJ1_001231"
     CDS             complement(10525..12972)
                     /locus_tag="ACHBJ1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430186.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12959..13348)
                     /locus_tag="ACHBJ1_001232"
     CDS             complement(12959..13348)
                     /locus_tag="ACHBJ1_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM9430187.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13408..13911)
                     /locus_tag="ACHBJ1_001233"
     CDS             complement(13408..13911)
                     /locus_tag="ACHBJ1_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM9430188.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13924..14148)
                     /locus_tag="ACHBJ1_001234"
     CDS             complement(13924..14148)
                     /locus_tag="ACHBJ1_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430189.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14252..16162)
                     /gene="ltrA"
                     /locus_tag="ACHBJ1_001235"
     CDS             complement(14252..16162)
                     /gene="ltrA"
                     /locus_tag="ACHBJ1_001235"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9430190.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16908..17045)
                     /locus_tag="ACHBJ1_001236"
     CDS             complement(16908..17045)
                     /locus_tag="ACHBJ1_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM9430191.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17042..18226)
                     /gene="mobT"
                     /locus_tag="ACHBJ1_001237"
     CDS             complement(17042..18226)
                     /gene="mobT"
                     /locus_tag="ACHBJ1_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM9430192.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18485..18775)
                     /locus_tag="ACHBJ1_001238"
     CDS             complement(18485..18775)
                     /locus_tag="ACHBJ1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430193.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18765..19907)
                     /gene="dcm"
                     /locus_tag="ACHBJ1_001239"
                     /pseudo
     CDS             complement(18765..19907)
                     /gene="dcm"
                     /locus_tag="ACHBJ1_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19983..20147)
                     /locus_tag="ACHBJ1_001240"
     CDS             complement(19983..20147)
                     /locus_tag="ACHBJ1_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430194.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20140..20814)
                     /locus_tag="ACHBJ1_001241"
     CDS             complement(20140..20814)
                     /locus_tag="ACHBJ1_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9430195.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20892..22232)
                     /locus_tag="ACHBJ1_001242"
     CDS             complement(20892..22232)
                     /locus_tag="ACHBJ1_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM9430196.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22315..22650)
                     /locus_tag="ACHBJ1_001243"
     CDS             complement(22315..22650)
                     /locus_tag="ACHBJ1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM9430197.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22660..23034)
                     /locus_tag="ACHBJ1_001244"
     CDS             complement(22660..23034)
                     /locus_tag="ACHBJ1_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9430198.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23047..23361)
                     /locus_tag="ACHBJ1_001245"
     CDS             complement(23047..23361)
                     /locus_tag="ACHBJ1_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM9430199.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23474..26701)
                     /gene="fss3"
                     /locus_tag="ACHBJ1_001246"
     CDS             complement(23474..26701)
                     /gene="fss3"
                     /locus_tag="ACHBJ1_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM9430200.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7871 a         5585 c         4684 g         8834 t
ORIGIN      
        1 attagacctg cttttttgag aaaaaatagt cgtgtaaaat taagaattag ctttgaaaaa
       61 aatacaaaga gaatagtagg tgtacaactt tgtagtcaag aagatttatc tggactatta
      121 tcaatgtttt ctcttgctat agaagaacaa attacattag acaaattgaa aatgttagat
      181 tatttatttc atcctagttt tagccaaccc tataatttta ttttaaaggc agtaacagga
      241 ttaaacattg aaacataatc gttactaaat gaaatcaaga aatatactgt attactcaat
      301 tcattgaaaa tatttatcat aatttttaat agaaaacact attaaagaga aaaaagagag
      361 gtaaatcaaa tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc
      421 cttatagcaa cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa
      481 gaaagaaccg agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt
      541 ttgacaactt catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca
      601 tcaagtgaaa gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt
      661 agtacgactt caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca
      721 agtaccgaat cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca
      781 aaaccaaacc atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa
      841 actttttcga caccacagca aatagaatca acagaatctt tccaatcaca accaatacta
      901 tctggtaatg aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa
      961 attggtgagt ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt
     1021 gcacaagcga tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac
     1081 aatttatttg ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa
     1141 gatttgggta atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat
     1201 gaggaaagct ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat
     1261 ttttatgcag gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt
     1321 acaggacggt atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc
     1381 tacgatttaa ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac
     1441 caagtgccta ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt
     1501 caccgaggac ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt
     1561 aaggttgtta aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa
     1621 gatggatcaa ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat
     1681 gttttgcaag gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac
     1741 cttcatttgg aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa
     1801 accttattat tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt
     1861 ggattcttag acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg
     1921 tttgacaaat aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct
     1981 catattttga gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta
     2041 cgtatcgttc agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa
     2101 tatttttttc tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg
     2161 gtaggtgcag tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat
     2221 ggatataaaa aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca
     2281 aacttactat caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa
     2341 tataaaaatt tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg
     2401 cgaagtgaag gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta
     2461 tcggcaaagc gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag
     2521 catcacattg aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac
     2581 tgttcatgga aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt
     2641 tatagtaact tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca
     2701 aggacatgtg ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat
     2761 acaagtggtg ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta
     2821 aaaaaaaaga ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga
     2881 aaagatgctg aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa
     2941 ttattgtgga taataattaa cagtgatata taaacatcta taatatatta ttcatactat
     3001 tgatatttag tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt
     3061 ggaaaacaaa taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa
     3121 tttgcttacg tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt
     3181 ttagaaattg ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa
     3241 ataaagcatc tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt
     3301 cctatggcta gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc
     3361 tacacgatca ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc
     3421 aaaaaaatcg ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg
     3481 atggcagtat tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga
     3541 atcaagcgga atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt
     3601 ttggtaaaat tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct
     3661 ttgtcttggg atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac
     3721 gggtggtaca agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg
     3781 aagtctttcc catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat
     3841 taggctcgat tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt
     3901 acattttttg gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct
     3961 taccaaaaga tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg
     4021 cggctacgat tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc
     4081 atccagtccc ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag
     4141 ttgaaaagaa agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta
     4201 ccatgagctt gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga
     4261 ttccttttta tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga
     4321 tggtctttcc tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg
     4381 ttggcccgga gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt
     4441 atatgttttt gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg
     4501 gtgccggcaa tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta
     4561 tcgaaaactt gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca
     4621 gcgggatttc gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc
     4681 aagtaactaa ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct
     4741 ttttaggcag ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa
     4801 aacaaggagg tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc
     4861 tgcgtatggg tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc
     4921 gaaaaactca agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac
     4981 aagaagactg cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt
     5041 ttagaggcta ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac
     5101 ccagattatg taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat
     5161 ttccgtctag tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa
     5221 ttcgagaatg gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta
     5281 tcaatatttt gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt
     5341 aggaacaacg gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac
     5401 tcttttatca agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct
     5461 ttagcatttt tgatttaggt tccttttcta attataataa gatgaccact acagtaacct
     5521 actgaaatat ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat
     5581 cttcattctt atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga
     5641 ttttacttcc aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct
     5701 gaaaagtggt gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga
     5761 atttgctatg aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga
     5821 attacttgcc acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg
     5881 aatagagcat acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc
     5941 tatccccgta gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat
     6001 ttttgaccct tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt
     6061 taattcggtt ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca
     6121 ttcatctaag gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc
     6181 tgacattaca tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc
     6241 aagaagaaaa actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat
     6301 atagactgat tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc
     6361 gagttaccga atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata
     6421 agttacacta cttataactt ctaataaaat tagattgagt cattattatt taacatcaac
     6481 gattttccag tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt
     6541 tgatacatta tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag
     6601 ataaactgga ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc
     6661 attttcaaca taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt
     6721 ttctaaaaaa tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac
     6781 ttgttttgct tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac
     6841 taaatgattc ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg
     6901 cgtctttttt ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga
     6961 gtgtttttca acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc
     7021 tcgaaccata tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact
     7081 cattctcttt tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc
     7141 ctctgtaaaa ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc
     7201 atgagttgta tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca
     7261 agacaataaa agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg
     7321 ttcttttttc ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct
     7381 ttttcctctt ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt
     7441 ttccgttaga ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc
     7501 gtaaatgccc acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa
     7561 atcacctggt ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc
     7621 cacccgagga agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc
     7681 aaaccccgtt tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc
     7741 ctcatcaaaa atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt
     7801 cactaataaa cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat
     7861 cgcgattggg ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc
     7921 agctaaagca aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc
     7981 atagttatag gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac
     8041 caaagacgca aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg
     8101 tggtaatccg gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac
     8161 ttgaataatc gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt
     8221 ttccaccatt gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt
     8281 gccagaacta ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat
     8341 acccataaaa gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat
     8401 tttttctcac ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg
     8461 gtctgctttg gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct
     8521 gattggcttt tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga
     8581 ttcagatttg ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga
     8641 ggttgagctg ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct
     8701 tcactcagta tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct
     8761 cgcaaatcca ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct
     8821 tttttcgact tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt
     8881 acctgatcct ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg
     8941 ccgatttttc tcccagccaa attcattcgg gattgtttct tattatttga aactggattc
     9001 tccttagtta agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga
     9061 gaagaaccgg atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact
     9121 gctgcaccac caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt
     9181 cgattcatta acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta
     9241 ctctggaggt tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc
     9301 acaatttgca gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc
     9361 gtggagatgc taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta
     9421 ttgaataaac gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat
     9481 aagaaactaa ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata
     9541 atccctgtta gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc
     9601 acggtcccta aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca
     9661 atctctgcct tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat
     9721 agaatcttgt tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt
     9781 agtaaccaag gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta
     9841 tcttttcctt ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct
     9901 tcactaacat ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg
     9961 aacgaacccg atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg
    10021 gtttctcgtt ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat
    10081 aatagaaacc caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat
    10141 gtttggatat tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc
    10201 tcttgaacca cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta
    10261 gtaatggcat aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc
    10321 aaccaatccc aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg
    10381 gaatatagat ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta
    10441 ccaactaaac tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc
    10501 cgaaaaagtt tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc
    10561 aaaggcatgg aagagttctt caaagactgg atgtacctga accacgccta cccgaccata
    10621 caaatcctgc atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc
    10681 atcttctcga tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact
    10741 gcgaaacgca aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact
    10801 attttgcgtg acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt
    10861 cgataatgtt ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat
    10921 tttgaagatt tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag
    10981 catggaaaca ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg
    11041 taaaaccaaa tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt
    11101 aatcgaacca tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg
    11161 atccgcgata ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa
    11221 taatccccgt tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt
    11281 ctccccatct cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc
    11341 agaatccttc tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt
    11401 actagttaag ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc
    11461 accccgttcc ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata
    11521 aactaagaga ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc
    11581 tagcgcattg gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac
    11641 attgcgcccc gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg
    11701 agtggcgcca aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt
    11761 catgtatcgc ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg
    11821 ccggactagt tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt
    11881 tagttcatct actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc
    11941 tttggactga tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt
    12001 ggaatttgtt tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt
    12061 cttattgcga acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt
    12121 gtcaattggg aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc
    12181 catttccccc acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg
    12241 ttccatgcat aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc
    12301 gtaacgtttc accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc
    12361 ttcaaatggt gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg
    12421 tgtgactcgt ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt
    12481 ggtatatcgg cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt
    12541 cactccatag acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt
    12601 gacctcttca tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact
    12661 atcgccaatc atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc
    12721 tgttaatcgc ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc
    12781 tgtggcaatt tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa
    12841 gttatcgtgg acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa
    12901 ctcatagtag gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt
    12961 aatcgggtac atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct
    13021 gttctttgac tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag
    13081 atcgtaaaaa tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa
    13141 ctgccaccgg tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca
    13201 catgggcaaa tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag
    13261 tcacaggaaa aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa
    13321 tactggtgta gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta
    13381 gaaatcaact acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt
    13441 taccaaataa ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt
    13501 cttcaagtgt tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac
    13561 atcactcata tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc
    13621 ctcaaaagaa ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc
    13681 ttcaagttcc tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc
    13741 aatctcaaag ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc
    13801 aattcgttct ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc
    13861 accttcgtta tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc
    13921 cccttacgca ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc
    13981 attaaagacc aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa
    14041 ctcccgctta aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
    14101 ggcattggtt aaaaaccatt gatatatgga taggtaagtt cctgatatta taaatatctc
    14161 aaacttttcc ccccattcac acggagcgtg cgactttcac cgcactccgc gttccatcgt
    14221 gtttatattg aaaagtattt atgtagtaga cctatatcaa gtttaattct tgattgccag
    14281 ctaatactct atatttattt agtcttatga gctggacttt atttagcttc aatagagcta
    14341 tatatttttg tattgtttct tctgttttag cgtgtattag acggtgtacc gcctctttta
    14401 ccaaaataag atttttataa tcgtcatttc caccagctcc agttggtaca atatgatgac
    14461 aatgtatttc attagcttgt tgaaattctt ccagagttat agcacatttt ccatgttgag
    14521 cagaaaatag cgaaattcga ttgtctgcgt attctattgt gcgattttcc aaagataatt
    14581 tcattaactg gtacatcagt aatttatcaa cacttagatt ggtgtggata aattttcttc
    14641 cctctagagt gtagcaattc gctaaataca cctttgccat tggttttctg tgttggatat
    14701 atccaacagg gaaaataggt tcgccctcgc cagcaacata ccgaagcatt ttagatttac
    14761 catatcttgt tgcctccttt gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt
    14821 tgtttgtacc taatctgtta gtgaagatag ccattacagc tctattcagt ttcatacaat
    14881 ccagatttac attcgttgca atctgataat aattttgcat acctaatacc attgaattat
    14941 aaagtcttgt ttcttctaat tccgttttcc cttttgaggg agaagcaatt ttctttgcct
    15001 gtgataccag cttgtctttt tggtttttga attgtttatc acttatatgt gatttaacaa
    15061 ctaacttctt tcttctcggt atcattttaa tcttgaaacc aagaaagtcg gagtatcttt
    15121 ttctaacatt tacaattctt gttttttctt gtgatacttc caattttaat cgttgttcaa
    15181 tccattgagt gattgctatt ttggcttttt cagcactttc cttgtagcgg cagaaaactc
    15241 taaaatcatc tgcataacga accatataca tttcctttaa ctttgttttc ttcatttctt
    15301 tgtaagcgtt gcttttacat gggctaccgc ttttattgat atgaaccaca tagttttttg
    15361 taacaggatt ttcctgccat tggctttcta cccaatggtc taactcatta agaacaatgt
    15421 ttgctagtag tggggatatt attccccctt gcggagttcc tttctctgga tatgttattg
    15481 taccgttagt cattttgatt ggagctttta atatcctttt taaaatgaat atcaagtgct
    15541 tgtcccgtat tcccatagcc catatctgtt ttatcagctt ggaatggttc acgttgtcaa
    15601 agaaaccctt aatatcaaat tcaatcacat aatgtagctg gctgatttgt aggcggttat
    15661 aggttgcttt tattgcattt tcaactgacc tattaggtct aaaaccatag ctgttttcac
    15721 taaatttagc ttcgcagatt ggttctaata cttgttttat acattgttgt ataagcctgt
    15781 cccacataca aggaatacct agaggtctgg ttttaccatt tggttttggt atttcttttc
    15841 gcctcactgg cttaggtcga tacccatgtg gactgcctgc aacaatgtat cttacttttt
    15901 tgacaactgt ttctgctggc aatttgccaa tatctttaat tattgtaccg tctgttcctg
    15961 ctgttttact gcctgcattt gctttgatat ttcgatatgc cagtaatata ttttctctcg
    16021 ataggataat atccataaga tgagtgaaaa tatctccatt cttacttttg gcatataaat
    16081 catcaaaggt gttttgcatt tcgtaatatt cagcgtgtct taaatcttct acacacaatg
    16141 tttcattttt atttttaggc ataaggcttc actcctttac ttgaaattga gtgagctttt
    16201 ttatcttact cgtaatcctt attgaaacaa cataatactt ttctaatata tacacgactt
    16261 gagggcattc ctccattctc attacaagaa tttcttaggt tcgccctctg cttttcagca
    16321 ttagttcaac tgcttattgt tcttcgtcag tttattctct tgatgagaac ccaatacctt
    16381 tcctcgttcc gataatctta tctatacata tatatcctta ggtcactagc tataagcctg
    16441 ctggcttgat accgcctgta acgttatatg gcatttcata acctatattc ttactttgcc
    16501 acaccagcta cgatttctcg tacctttgca tttctacaaa gttcaaattt agacccgtac
    16561 attcgctgtt tcgtcagttc cttatagaac attctaacca tagatatttt atagaccccc
    16621 gacctgttgg atacgctacc cacctctgga tagttttcga tagaagatat atgttcatca
    16681 actattacgg tatctttcag ccgtctttac cgagcttcaa acattctgag tttcctcata
    16741 caatgcttgt cggaggctca gggcaggcgt ttcagagcgt tacttcatca ttccacctgt
    16801 cagattatca gttctcctaa taaccaaact ttttcgttct tttattagtg ccttagcttt
    16861 tcactaagga acgagtcgca cataaatttt gtccgaaatt catcttgtca ctttcctttc
    16921 ttttccattg ctcgatccgc tgctgcactt gcattcatca agcacatcac agtaacacca
    16981 agcatactgc caccaaatac caaaaaaata tcttttacaa ttcccatgac gattcctctt
    17041 ttcatataat caaattttca attgccatac tttgttgctc caaaatcttt ttatgtcggt
    17101 ctgataagcg tgcattctgt accatatcct ggatataggt tgtccgattc aatcgatcca
    17161 actccttagc cattttccac gtaggggcga cttgtcggcc tagccaattc aaggttcgtt
    17221 caatggtata cggttttggc tcggaagtta attgaatctc tttccgattc cgtccaataa
    17281 aacgccccca ttgttcattc aagggccagt tggttcgcct tttaccttct actttatccg
    17341 caaatcgcaa gtaacgattg ataatggaaa acgtggtgct ctcaatgcta cgacctttta
    17401 acaaatcttg aatcgcatga aaagcacggt catttttcag tcgaatctca aaacgatttt
    17461 tcgtctcagt ttggtctaat ggaatcccta acttgatata ttgttcatag tctttttcat
    17521 acaaacagaa ataaacctca ctcttgagac tgccgatata taaggtattg cccatgccgt
    17581 ccttttcatc agcctttaaa agttccccgg aacgataact tttaaaggta cgaaacaagg
    17641 agatacattc ttccttctga cattttttcg ttagctcagg tatatccaat agccccactc
    17701 gatcattgat tgccaagtcc aagcgtttca tcacgccacc ggcctttaga caatcctcga
    17761 aaaagtcgta ccagctacgc ttttgagcga gtaaaaaagt ttcaaattgt cggcaacctc
    17821 gcccctttaa ttctaaaagc acacctttat cttcttcatg agacaacatc accacaatat
    17881 cacccatgac atactgttct tgataggagt aaaaggcata atcttcatgg agcatatact
    17941 caaatttcaa atggagaatt tcttgaataa tttttcgtgc atccgttgtg ggaaagcgaa
    18001 tccttacata gtcaaacagt aacgtcaaag gcaaatcggg attaaagctt tccagttggt
    18061 caaaaatttt cctcatggtt tttatagatg cggttacgcc accattttct atctgattta
    18121 agtacggccg actaagtcca caagccgtag ccaatttatt ttgtgaaacg ccgtattcgt
    18181 tgcgtcggtc ttttaatagc cgataatcta aattccgttg tgccaagttc tcacctccaa
    18241 attcaaggtg ttaacttttt caaatgagtt aacacctata caaattactt actatctttg
    18301 atacaacaag cattcttaat ggttgcttac ttattttttg tgtttttgta cccccctgtt
    18361 agatacgggg gtgagacatc atggtcggct aacgccgtcc atgaacaggc acagcaccac
    18421 ggaggatttc tgcttcgcga atcctccgcg gtcagcgcct aacagtcagt tgtgttggtt
    18481 taggttactc atctgtaata ccatttactt ccaactctgc tttcatttct aacaagtatt
    18541 gacgaatatc tggttctttt gcttggtccg caatccgctc aaattctgca attacttgat
    18601 ttatttcaat tcgagcatcc gtctcctcat tcattaaata tggatcgcca gcgactttga
    18661 caccattctt gcgaatatca aattttaacc gtcgcaattg ttcttctaat tctgggtctg
    18721 actgaatctt tccaatcacc caataaattt ctccaatcaa ctcatcataa accatgtaca
    18781 tccaactctt ttaatcttct ggcaatatca aaaatgaccg gaactgttac agagtttccg
    18841 gcttgcttat acaactgact atctgaattg acttgagaat ctcgatcaaa agcccaatcg
    18901 ggaaagccct gaagtcgcca acactctcta ggtgttagct tccgaatttg atactgatcc
    18961 gtgacgatcg cttgttggtc tcctgttagt aaggtatgaa cactctgttt tcccagacga
    19021 cctcggcgac ttgtggaatt aggaaaagcc agattcacac tgtcttctgg cttcacctct
    19081 tgatattctg tttcattttt tcgaatcatg atttttgtac caccttgttt cgttgacaag
    19141 gtgggggcgg ttccagtaac ttcataaact cgattacctt gttcaaactt tcccggtaat
    19201 agtcctgcta cagagattcc atcccttgct ggcagtgcga ttttgattcc ttcatcacct
    19261 gttaaggttg gcgctacgcc atctgaacca tagacttgtc cacccattcc tcgattggaa
    19321 ggattgatat ttaccaaatt tctgatgttt tgatcagatg ttgaagacgg tccaatgata
    19381 ggaaatactt gttcggtaca tgcgtctcga gaatgtccga taaggaatat ccgctcccgg
    19441 ctttggggaa tgtaggcctt tgagttaagc acctaccact ccacgtcata ccccaatcca
    19501 tccagcgttc ggaggatcgt ctcgaacgta gcccctcctt cgtgattgag caatcctcgg
    19561 acgttctcaa ggaataaaag cttaggtcgg agaagagtgg tgaaccgagc gatttcaaag
    19621 aataaagtac ctcgagtatc ggaaaatctt tgccgctttc ccgcaattga aaaagcttgg
    19681 cacggaaatc cgccgcaaag gatgtccact ggtccgaggg attgaataaa ctcatctgat
    19741 acacttgtga tgtcatgcat ttccacctcc ttacttgtat catgaattgc tttataacta
    19801 cgtctagcaa atttatcaat ttcacaaaaa ccgatacaat gatggctcgc ctgttccata
    19861 cctaaacgaa agccaccaat accggcgaat aaatctaaaa atctcataca ttacctcatt
    19921 cattgcaaag aaaaaagcca agaacatttt gctcttggct catacattat taatttgtcc
    19981 tactattttt tatttaatcc aactaatttc cgttgttttt cattggcctg atcggctaca
    20041 tatttaatca ccgttttgaa ctcttttgca gtagcatttt ccgataaatc cgcaatgctc
    20101 ttattgtatt tctttacaat tttcgttact tgttctttct cagtcatgta attgaacctc
    20161 caaatctttc ctactatacc ctagtggtaa aaataattct agattatccg tagcataatg
    20221 aacttcggaa atattattcg tataatcttt tattatgata tgaagcaata catctggatg
    20281 tttactcttt acttttgcaa tgttctctct tgatttgaaa aaagcgttaa taaatgcctt
    20341 tttcggcaca tgacgtccct ctgcaatttc acgttcttgt gtaaatttcc aagcgatttt
    20401 agggtcttga taaacataga aaatcgtcac tcgaaaatgt tttttcaacg cccgcgagat
    20461 atttttttca gcacttaata ttgcaaaagt tgcatccaga ataaaagaat agccatgatc
    20521 taagacatat ctaaatgcct gctctaccaa ccaagaagat gctttttgaa agagactaga
    20581 gttttttcca ttataatcag gaaactgggt gcgaaagtaa tccgcatcaa taatcacatg
    20641 gttatcataa ttctcagcta atccaatggc aacctctgat tttccagccc caggactccc
    20701 tgccataaaa actgcgtctc gtccaacttc gggggatttc cctttgataa tataagaaat
    20761 aaacttatcc ttatttgctt ttgcatactc taaataagaa tcattacttt ccatattaat
    20821 tatccccttt cttcctacta tataaaagta ttatacgtta cttgaagtat aaagtcagta
    20881 ttgaagatca ctcaatcatt catcttttct aatttactaa ttgaatctaa gaaatcatac
    20941 ccttttggta caagtggcgt ataaaactca ctaatcacac tgccacctgt atccacataa
    21001 cctcgacctt tgatgtgctt cataaagaag tttttttcga cctccccaaa catcatggaa
    21061 tatccgagtt cactcatacg acccaaagcc acgcgaaagt tgaattgatc gcggattccg
    21121 tctcctaaat atttcgcatc cggcctttgg caagccaaaa tcagaaagaa accagattga
    21181 cgacccaaca tcacgatttg tttgagctta ttcaaaatca ccgcactttc tttcgtcgtt
    21241 aacatctcca tataagccac gtattcatca aagattaaaa agttgggtgg aagtccaaga
    21301 taggcataat tctctcctgt tttgtagtta gacatttctt tcattgcctt gctacgagcc
    21361 atcatacgtt catagaaatc ttccacacaa gcagaaattt cttccttttg tgaataaaca
    21421 tgaggcatca ccgtacctaa atcagctaaa tctgcatttt tgggatcgag gataaacaat
    21481 tctgcatctg acttcaatag tgcttcgata atggtgagga ggaaataggt cttccctcca
    21541 cccgtgccac cagcgattaa catatggggt aaagaatcat aagcccatac ttgatttttc
    21601 atcaatcgca gagtcccatt ttctgccact acttcgtcta tcccaattcg attggcgatc
    21661 atatcataca gtaaagtata ctccacatag gagtctttga gttccttttc tactaactca
    21721 caatacagtc cgctttctaa cttcttttcc aattttaaaa gctgatcttg gtatttccca
    21781 agtgaaattt gaacgcgaat agacagtaat ccatctttca ttcgataaca gattttgggg
    21841 aagtaactga tagtctctct ggttcggcta ctattcaaat ctttgaaaaa gccttcactt
    21901 tgagtttgtt tcacttcata ccaatggttc tctagaatca tccgagctag cttttgtcga
    21961 tgccacaagc ttcgccaatg atcaagaaaa aagtgaatat aaagcaatat catagctcct
    22021 gaaaaagcga gacttccaag cagacatact aaatctttta tagaccaaac gatttcaata
    22081 gtagaaagtg atagcttaga aatatcaaat tgtctaaact cttgaaaata aaagacgctc
    22141 atccaaccga tgaacagagc gagtagccag ccaagaacaa agtgataaac caagtgctga
    22201 tctcctgcgc gtatgcgatg tcctttgtac attttgatgg tttccacctc caatcgaaaa
    22261 agcccaatag gatttcatta ggcaatttca aaaagttatg ctagtcattt actgctacga
    22321 ggaggggagt tgtttcgttt caaccaattg atttaaaaag ccttcaatag catccggcat
    22381 ctccacacca atttcttcca aaatggattc tgcctgccga cataagtttt catttacttc
    22441 gacaactata aagaaagact cttcttctcc taaccagtct agtacatcag agtcttcttc
    22501 actgtcgcta ttttctccaa gtaaaccttg atgtataatc agaaacattt tttcatcaga
    22561 ggttaatgct ttaacgagtt gttcctcagt cagctcgcct tttccatatt gatcgagaca
    22621 atctaaaata tgttcaacat taatttccat aatcttcacc tatttctttc tttcattttg
    22681 gttcgtagga tttccttgat tcggctgccg cttcaagact aaatcatccg ccttaatgaa
    22741 ccaattaacg gtagttcctt ctcgaaaaac ataattggca acggtatcca cgattggatt
    22801 gaccaactct acctctgcat cataagcaaa ctcgcgtaag ggtacattag ctgggatact
    22861 aacttgaatc attcgccctt gtgcagagga ttttaaatta taggttcgtt cttttacctc
    22921 attaccaatt gttccatcct cattttgctt ccgtacttct cggcgtaatg cggaaaaacg
    22981 caacttccca aatgttaaac ctgaatctac gacaatacct tctgttaatc tcatcttctt
    23041 ttcctcctat gctttcacta aatcatcaac atacaacacg taatcagtaa acccacgatt
    23101 ctcaatctca tagcctttgg caaccaaatg gggattcacg gcttttagcg gttggtcttg
    23161 atcaaaatcc ttttcaccgg cttccgcagc tactaccacc tcaatatcat cggcccgttg
    23221 cttactggaa tacaaatgat aactgcgacc aatcaccgta gtattccgtc catatccttc
    23281 tgttataatt tctccttcac cggcataact taactttcca aaggtttcag ccatatctgg
    23341 cacgacaaat ttcaattcca tcttcttttc ctcttttcat tgttagtttt ggatacaaaa
    23401 atagcgcgac tactttttta ggaggcggtc gcgcacgttg gttcttagga tttacttgta
    23461 tcatctcatc accttagttc cggcgattcc agaaataata gcccgccgtc gcaaaaatca
    23521 aagtaaaacc aatgaacagt aaaacattgg aatttttctc cccggtttga gggaaggtct
    23581 tcactgtggg actagattct tgagagccac ttggaacagt tggtgtttcc ggttgtttcg
    23641 gcgtacttag tatggttgga acttcttttg gtgttaaggt ttgagatttt tctttcaaat
    23701 cttcattgtg ttttccattg atattcccat ctttatcgta attgatttcc gcaaaagtaa
    23761 acttggttcc ttctggatat tttccggtat ctactttttt cgcaagcaca gttttggtga
    23821 attctttatc atttacttca taatcaatct tgccagattt ccaaatttct ttgtttgtac
    23881 catccggtaa taaagcatac agaattgttt cgaaagcttc ttttgagcca tccagtacat
    23941 catgagtaat cgacacatca tcaaacatat ccaccacatc accatgagta aaggtctgag
    24001 aaccgttttc taggtgggcc tttgtttgga tagaaacatg gtgttccact gtacaactga
    24061 cagtttgggc ttggttattc aagctaacat ccttcgctac tggcttatcg cctgcttcaa
    24121 aagcttcctt attttcatac acatagttga ataagacaat agttttattt aaaacttgtt
    24181 cggcggttaa tttatgctga attttccaag ttcctgtttt ctctttgttc gccaacgttg
    24241 cgcttgtttc tgcaatcacc actggtttgg ctttttcatc tttttcttgg gcagcttttg
    24301 tcgcatccac atcaatggct tgcgctacca cataccagtc ttctttgatt tcatgtagat
    24361 tgtaactcaa tttatcaact agctcggtag aatccagaga agttaattct ttgtttccgt
    24421 ctttccaagt cgcaagagaa gttaaactat cttcttcctc aggcaaatca tagagcatta
    24481 aacggttcaa actaacagaa aatgctttgt tcgtcagctt ctcgtaagga acggtcaccg
    24541 ttttaattgg ttgtttttgg tcttgttcgg taatggtaaa gacatactca ctcttcacaa
    24601 agtcttcttt gttttcctta aaagtagaac ggatttctaa tggcgtaatc ttttgaaaac
    24661 cttctggggc ttctacttct tcaagtaaat aatccccata aggaagattt tcaaacttgc
    24721 cataaccatc aaaccctaat tgctcgttat aagctgtggt cgcttcatct tcggcacctg
    24781 tgatttcatt ggtcccttcc aatggcgaca ctttaaaggt cagatcattg aagccagttt
    24841 cggtagtgcc cgcagctgat ccagcaaatt taaagaaatc aaagccaaag cgaatcactt
    24901 gttcttttgc cgtaacattt cgggtaatca cggcattttt ttcgctatca tccacctttt
    24961 tgatggatac aggatacttt gtttcatcaa ggctatatcc ttcgggcgct tttgtttctt
    25021 gccaataata ctcgttgata gctaaatgtt taacggcaac ttggttcttt tcgtctaaag
    25081 tcaaagtgac cgtttcatca gaacctttcg ttcccttcgc taactctggt ttaaaagcct
    25141 cactccattt aacagctttt ccatccttag cagtaaacaa ggtgtattca gcaccctcaa
    25201 agactgcctt accttgtgct ttatccccag tatctttgtc ttctttcgtc aaagtggttt
    25261 cgccagtgac ttcttgattt tgccctttta cgttactggt aacaggggct acggtttgat
    25321 tggcatactt caattcgact tttacaggtt tgaacgtatt cacgaaccca tgactagctt
    25381 tagtttcggt cacataatag gttcccaatt ccaacgcatt agcaatctct tttggtgttt
    25441 ccgcatgacc gttttcatcc gtggtcattt cttgaacgat ttcaccggtt ggactgtctt
    25501 tgcgaatggc aaacgtatta ccagctaagg aataattgtc attccaaaga tcactccctg
    25561 tttcaactcc gcttttatcc agaatgattt gccctttttc ccgtgcgttt tttgaagtca
    25621 cgtaaatggt ttcgcctgct ttaatggtgg tagtcatggg ggtggtatca atcgtataag
    25681 gcgccggtac tgatttttcg gtaatggtta cttttgtacc atgaggaatc tcatccaatg
    25741 tagcaatgcc ttctttgtca gtagtcacgt cttttgcagg taaagttttt ccaaagtcta
    25801 aatggaaaac cgttcctggt acaacatcac ccgattcttt atcgactttt ttgatcttta
    25861 aagaaccctc cgtttccaca tcaattttga ttgtgtaggt attaggttta tcaatcgccc
    25921 cggccattaa agtctgttgg ccagcctttt tataggcgac aggtgttccg gtaccagcag
    25981 atttcttaag cgtcagcaca ccactttctt ttgaattggc atttggggtg attatcaact
    26041 gattaccatt cacacgataa tcaatgtttg cggcattttc cacaacctca tcgaactctg
    26101 acaaattcag attattcgta tccgtcacag tagtcgattg ccctaatact gttttagccg
    26161 tactattatg gaaggttggt ttcttctgat aatcgtcaat ggcttgattt atttttgcct
    26221 caatggctgc gatatttacg gcactaccat ctgatcgttt tattgagtga agggtatacc
    26281 cattaacttc ttgccagatc atcttctgtg ccacaatatg agtatctgca tctgtaccta
    26341 cttttttcca taaaacggaa actaatttgg ctttatcgga catatctggt aacgggtttt
    26401 tctcatagcc aggagtgaac tcattgtaaa tcgggactcc tggttcaata cagaaaatat
    26461 cttgtttaaa gccgttgtac actgcataat gcggaacagt gacttctgac attggacgac
    26521 catcactaaa agttccttca acagcatatt gatgtgctaa atctagttct acggttaccg
    26581 tttgtggatg ggtaatttca tcagcaaaag taatcgtagg agcaattgct ccaacaattg
    26641 cttgaacagc caaactcaaa atagttaata cacataattt ctttttttgt tttttataca
    26701 tgaaatgttc tcctttcaaa ttaagcacaa aaaaataaga tgctcgcttg aacatctttg
    26761 tttataaagt ctttttaaaa tttttattga ctgaatagaa tggatttgag aatgaatgag
    26821 cctaaaaatt taatctaaaa tacgaaatat actgccttta aattagaaca cattttagaa
    26881 cacaaacatt tcaaatttaa tcaaatataa taaaatatat ttacgggaaa acctttataa
    26941 atcaaggttt ttcaaactta ttcaatgata cgaa
//
LOCUS       DAYYNQ010000032        26141 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000032 DAYYNQ010000000
VERSION     DAYYNQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.032"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACHBJ1_001247"
     CDS             <1..318
                     /locus_tag="ACHBJ1_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9430201.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
     gene            791..1345
                     /locus_tag="ACHBJ1_001248"
     CDS             791..1345
                     /locus_tag="ACHBJ1_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430202.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1518..1991)
                     /locus_tag="ACHBJ1_001249"
     CDS             complement(1518..1991)
                     /locus_tag="ACHBJ1_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430203.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            2003..2950
                     /locus_tag="ACHBJ1_001250"
                     /pseudo
     CDS             2003..2950
                     /locus_tag="ACHBJ1_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3221..3656
                     /locus_tag="ACHBJ1_001251"
                     /pseudo
     CDS             3221..3656
                     /locus_tag="ACHBJ1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3672..4505
                     /locus_tag="ACHBJ1_001252"
     CDS             3672..4505
                     /locus_tag="ACHBJ1_001252"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430204.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4750..5775
                     /locus_tag="ACHBJ1_001253"
     CDS             4750..5775
                     /locus_tag="ACHBJ1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430205.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5913..6038
                     /locus_tag="ACHBJ1_001254"
     CDS             5913..6038
                     /locus_tag="ACHBJ1_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430206.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            6063..7178
                     /locus_tag="ACHBJ1_001255"
                     /pseudo
     CDS             6063..7178
                     /locus_tag="ACHBJ1_001255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            7261..8157
                     /locus_tag="ACHBJ1_001256"
                     /pseudo
     CDS             7261..8157
                     /locus_tag="ACHBJ1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            8173..8997
                     /locus_tag="ACHBJ1_001257"
     CDS             8173..8997
                     /locus_tag="ACHBJ1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430207.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            9029..10339
                     /locus_tag="ACHBJ1_001258"
     CDS             9029..10339
                     /locus_tag="ACHBJ1_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM9430208.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10487..11746
                     /locus_tag="ACHBJ1_001259"
     CDS             10487..11746
                     /locus_tag="ACHBJ1_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9430209.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11756..12628
                     /locus_tag="ACHBJ1_001260"
     CDS             11756..12628
                     /locus_tag="ACHBJ1_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430210.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12640..13470
                     /locus_tag="ACHBJ1_001261"
     CDS             12640..13470
                     /locus_tag="ACHBJ1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430211.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13510..15693
                     /locus_tag="ACHBJ1_001262"
     CDS             13510..15693
                     /locus_tag="ACHBJ1_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9430212.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15754..16974
                     /locus_tag="ACHBJ1_001263"
     CDS             15754..16974
                     /locus_tag="ACHBJ1_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9430213.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16971..18431
                     /gene="gtfA"
                     /locus_tag="ACHBJ1_001264"
     CDS             16971..18431
                     /gene="gtfA"
                     /locus_tag="ACHBJ1_001264"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM9430214.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18508..19614
                     /locus_tag="ACHBJ1_001265"
                     /pseudo
     CDS             18508..19614
                     /locus_tag="ACHBJ1_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19936..20145
                     /locus_tag="ACHBJ1_001266"
     CDS             19936..20145
                     /locus_tag="ACHBJ1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9430215.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            20138..20287
                     /locus_tag="ACHBJ1_001267"
     CDS             20138..20287
                     /locus_tag="ACHBJ1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM9430216.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20441..21106
                     /locus_tag="ACHBJ1_001268"
     CDS             20441..21106
                     /locus_tag="ACHBJ1_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430217.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21390..22475)
                     /gene="xerS"
                     /locus_tag="ACHBJ1_001269"
     CDS             complement(21390..22475)
                     /gene="xerS"
                     /locus_tag="ACHBJ1_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM9430218.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22809..23042
                     /locus_tag="ACHBJ1_001270"
     CDS             22809..23042
                     /locus_tag="ACHBJ1_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM9430219.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            23039..23446
                     /locus_tag="ACHBJ1_001271"
     CDS             23039..23446
                     /locus_tag="ACHBJ1_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9430220.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24583..25101)
                     /locus_tag="ACHBJ1_001272"
     CDS             complement(24583..25101)
                     /locus_tag="ACHBJ1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM9430221.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            25468..25716
                     /locus_tag="ACHBJ1_001273"
     CDS             25468..25716
                     /locus_tag="ACHBJ1_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430222.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8926 a         3984 c         4844 g         8387 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt
      361 gacgctcccg acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt
      421 attaatgaaa agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa
      481 ggggagttct tagtatattg ttcaaactta aatttcatat aatttaatta tgtaaattag
      541 ctattctaag aacttctacg gataactgcc cgtctaaaat ttaaataaga gcttagaatc
      601 ttcatataag taaggttaat ttttttgtac aaaaaagtcg gaatattctg agttactgaa
      661 atgatataaa atcataaact aaatcccttg attttattga ctttcaaaag tttatatata
      721 taagttaatg attatggtaa aatatataca taatcattaa ctcagaatat ttttaagaag
      781 ggaaacaata gtgagctata atgtacaacc gttaaggaca aatgaagaga tacaagattt
      841 tcagttttgg ctaagaagaa caagtaatcc ggaaagagat agttttttat ttttgtttgg
      901 aatcaataat gggttacgta tgtcagatat tataggatta aaagttggag atatacgagg
      961 caaaagtaag cctattattg ttgaacataa aacaggtaag cgaaaaccga tttttataga
     1021 taatttgaga gaagaaattt tattatatac tgaaggtaaa gaagaaaatg attggttatt
     1081 tccgagtcgg caacaaggtc gacatataac aagggatcgt gtctatcaga tatatgcaga
     1141 tatagcagaa aaattaggac gagatgatat aggaacacat acattaagaa aaacttttgg
     1201 gtatcactac tacaagaaaa ctagagacat tgctacctta atgtttattt ttaatcatag
     1261 ctcgcaagca attacaaaaa ggtacattgg tataactgaa gatgaaatag gagcatcact
     1321 tcgtggattt aaactaggtg tataatttaa aaatttttat aaacagtaaa gacaaagatt
     1381 aaaagctatt tttatctaga acgattttat ttcataaagt tcgcggaata aaagctgggg
     1441 ttactggtac ttggtatagg ttcgtgttat ataaactaag taattaaata acatttttag
     1501 gttggaaaag gatgaaatta aaaaaagaga atcaatacgg tctcaaaaac gcaaacgctg
     1561 cggaataaga aaaatactga taactcaacg tttagcagac tatctaatag ggatagtctg
     1621 cttttttcgt ttttgaccat acttttgatc ttctgcaaac ttccttgctt ttcctctgta
     1681 gaactcatga aatctgcaaa acgaattgcc gtttcctctg tctgcttttt cgcgatatgg
     1741 gtgtatatat catccatagt ggttttaata tctgtatgtc caagtcgaac atacacttct
     1801 ttgacagacg caccagattc aaagagcaga ctacaatggg tatgacgaaa accatgaagt
     1861 gtgatatgtt ttaagttatt ctctttaatg atgattttca aattgtgatt tacatagtct
     1921 aaataaaggg gcttattttc ttctgatgaa aacaaaagtt gttcaccatc ttttataggt
     1981 ttgattctca tttttagata ttatgcccgc acgtattcat cacatttcta ttgtgaatcg
     2041 ctttattcga ccgacatttg acttttatca taatatactc ggtttaaaat tactaatgaa
     2101 aacaattaat caagacgatc ataccatgta tcacctgttt ttttcagata atcaccaccg
     2161 tgtgggaact gaactgactt tttttgaagt acaagaaggg aacaaccagt tttttggaac
     2221 caatacgatt gaacgaacga tactaaaagt tccttccgaa gcatcattgc atttttggga
     2281 aatatatttt gaaacacaag gcgtttgcca ttatggaatc gaatcatttt ctggacgacc
     2341 tatcttacgt tttgaagggc cagatgcaac acgattagct ttagttccct tacgcgaatt
     2401 tgaagatatt gatgattatt tcccgtatgt cacagaccag atccctacag agcatgccat
     2461 tttaggaatt gatgccattc aactacgcgt acaatattcc gatgcgactg aacgagagtt
     2521 attgtcggta ggctggtagc acaaagaata cgtcagtttc ttttcgggtc atcaacgagt
     2581 gacaatctta gaaaatcaag acactcagtt ctatcaagaa gttcagatta ttcaagatcg
     2641 tgagaatcca gttgccattg aagggattgg tagcgtccat catgttgcgt ttggcgtaga
     2701 aaataaatct gatttacaac gcatcgacaa acaattacaa gagcgcaatt ttataaattc
     2761 aggaataaaa gatcgtgaat tttttgtatc actttattat cgtgacccaa atcaattatt
     2821 gattgaaatt gctacagggg aaggaaacct agatgcaaaa gcgtatgaaa atcaaagtcc
     2881 acgctttgaa gaaattcctt tatttttacc gcagtactta aatttcattc gtgaacaagt
     2941 ggagcaataa ttagctaatc aaatccatga cgaagagtaa gaggttgact cataagtcaa
     3001 aaaataaaga aaaacgcatc aaatccattc taaagaatgt tgatttgatg cggttcctat
     3061 ttttataaac tatcattttc gactaataaa actacttatg agtcagcctc tctttttttc
     3121 ttgattttta tacctctttc taggtaaact aaacatctgc caaaaatacc gtgtctcatt
     3181 gaaacattct caactatttc aaaataaagg ggagtaccaa atgaacgagt atattgaaat
     3241 tatccacgca agagaaacaa cttaaaaatg tcagtctcaa aattcccaaa aataaaatta
     3301 gtatttttac cagcgtatct ggttcaggaa aatcatcgat tgtttttgat actattaccc
     3361 aagaagctgg tcgtcaactg aatgacacct atgatagttt tacccgttta tttttaccta
     3421 aatacagtcg tccagaggtt gatgagattg ataatctttc tacggcgatt gtggtcaatc
     3481 aaaagaagat tggtggcagt gcgcgctcaa cgttagggac gattactaac atcaatgcgt
     3541 tatttcgtgt gttgttttca tgttttgcaa cacctagttt aggatatgca aatgcttatt
     3601 catttaatga tcctagtgga atgtgttctg tctgtcaggg aatcggtaag acgattgcat
     3661 ttttaatagg attgctttat ttttcggcta ttgataatgc tgattttgta ttttctgatc
     3721 catttaccgc agaagttttg tctggaattg aggaacaggc aaagaatcat ggttattttg
     3781 tattggtgca taatgttaat tcagcagaag atgttaaaac tattcagaga aattggcgtt
     3841 ttgaggggtt cattgttgtt ggtgtacgtg taaaagaatt tttggaatta aataacgttt
     3901 ttgatggtcc tgttgtttat attgatacgc atctttcaaa agagacatta agagaacttg
     3961 aacaacaaat gaatcgttta tttgtaaata caaatgatta ccaagctagt acattggcag
     4021 tggaacattt gattcattat ggtcatgaga aaatagcgtt tctttctttt gactacattg
     4081 taggtgaacc aagtgttatt caagaacgac atcaagctta tatcgataca ttaaagaaat
     4141 atcatttaac ctttaaatcg cattggatat ataacaactc tgaatttgag cgaatttatc
     4201 aagaattagc tgaatttact gcaattgttg ttactggaga ttatttagcg gctaaattag
     4261 taaaattttt aaaaaataag aataaacgag tgatggaaca attatcgatt attggatttg
     4321 atgatattac ttttgcagaa ttaatggatc ctgcgttgac aacaatacga ctaaatccag
     4381 gtaacaaggg ggcgattgct tttcaccaac tgattggact ggccaaacaa gaagaaataa
     4441 aaaatcaagt actactatta gagggagaat tgatttctcg agactctgtt cacaaaatca
     4501 aataaaaaag ctctgtgaaa taagtatagt taacaataaa atatccgaac gatagagaga
     4561 atgaactgtg atctagcagt tcctcgtcgc aaattcacta gaattagcat tatttactct
     4621 aaattgttcg gatatttttt cttgttttta attatgatta cagtgcctat ttttaacgaa
     4681 atttgcttat tttcaaaatg tatgattata tgttagtaaa cactttcaaa tatgaaaaga
     4741 ggagctatta tggtaggaat tagggatgtt gcaaaacggg cgggtgtgtc cattgcaaca
     4801 gtatcgcgcg tgctaaatga ggatccgaca ctttcagtaa ccgatgaaac caaacgaaag
     4861 attgcagaat cggttaattt ttatcattat gtcaaaaaaa gtcccgttgc aaaaaattta
     4921 acaaatattg cattaataac gacagtttct gaagtagatg agttggaaga tccctatttt
     4981 cgaacgattc gcagagggat tcaagtcgaa gcagaacaaa gcaaagtcaa tttgaagaaa
     5041 attattcgtt tgtcagaaac accgttggag caagacgatt tgctaaaatg tcaaggtgtg
     5101 ttgattattg ggcaggtgct atcttctgtg attgaagaag tatcagcaat taattcgaat
     5161 gtcgtagtga ttgatgaccc tagtattgaa gctgaagtag atgcagttta tactgattta
     5221 acaaaagcaa cgaatcagca tttagatcga ttgtataaaa aaggacaccg aaacattgcg
     5281 tttatcggag gaggacgtgt tcgtttagat agccaaggaa ataaacacgt gtcagatgat
     5341 gattatcgaa aaatagccta tgagacgtgg atgaaacaaa aaggattaga aaaatatagt
     5401 caaatttatt tagaaggctg gaccacactt gacggaatgc aggcagcaga tttacttttg
     5461 aaaaaacatg ctaaagataa gttgccgact gctatcatgg taggaaatga tcctattgct
     5521 gtgggggttt atcgttcact tcaaaaaaat ggtctacata ttccagaaga tatttcgatt
     5581 gtgagttttg ataatattga agttgctgaa tttttaacac ctgcattaaa tacggtgaat
     5641 gtgaatacag aagaaattgg aaaactagca gttcgcatgg ttaatgaacg aataaaagaa
     5701 gttagaaatg tttcgatcaa agtgattgtt tctaataata ttattgttag agaaagtgaa
     5761 aaaaatatcg attaaaaaag taaacatcca aaaaagggtg tttacttttt tgtttactaa
     5821 tatttttagt aaaaaatctc gacaaaacat taagtaaaca gttatggtgt tgttaacaaa
     5881 aagaaaacgc attcaaaaca ggaggaatta agatgaaaca tttcaaaaaa atagctttaa
     5941 cagggatagc tgttttaact gggacattac tcgcagcatg tggtggggga gatagtaatt
     6001 ctaaagacgg gcaagtaact ttaagctatg cgagttgaga tactgaccaa gctccaggac
     6061 taagaaaaat gttagatgaa tttgaaaaac aaaatcctga tatcaaagta gaaatggaga
     6121 caacgccttg ggatcaatat tgggttaaat tagaagctgc aacaacggga ggtaatatgc
     6181 cagatgtagt aaccatgcac tctagcgaat cctataaata tatgtcacag aatgctttga
     6241 tgggacttga tgatgttatc aaagaaaatg atattgacat gggaaacttt actgaaggaa
     6301 ttgctgattt ctatacgctt gaagacgaac tatacgcaat ccctaaagat gcttctgtag
     6361 tggggctatg gtacaacaaa gaattatttg ataatgcagg tgtcgattat ccagatgaaa
     6421 catggacatg ggaaacactc agacaagctg caattgattt aacagatgaa tcaaaaggca
     6481 tctatggttt tgcatcacat attggaacag agactggcta ctggccattt atttatcaaa
     6541 atgggggaga agtatttgcc gatgacaata caaaatcagg tttgaatacc ccagaagcac
     6601 aagaagcttt agctttttat cgagatattg cacttgttga tcatgcttca ccaacaatta
     6661 cagaattaca agatcctgat aagttaagtc gttttcaatc aggtagatta gcaatgatga
     6721 ttgaaggaaa ctggtttgca ccgggatttt tgaaaaatga ctatctagtt gaaaatgttg
     6781 cttctgccgt gttacctaag ggaaaagtta atgcaacaat tacaaatgga cttggttggg
     6841 ctgccagtgc gagtacaaaa tacccagaag aagtgaaaaa actaatggca tttttagcta
     6901 gtgaggacgc caataaaatc caagcggaaa cgggggcatc aatcccagca ctaaaaggct
     6961 ttgaagacga gtgggctgct tcttcagata caattgattt gagtgctttt ggaaaaatgt
     7021 tggagtatgg agtccctcgt ccatacaata aagatggatt acgagctgaa caagcagaag
     7081 ttcaaattat taaccgtatt ctgtctggtg aagtaactgt tgaagatgga accgctcaaa
     7141 ttgaaaaaga agtcaatgcg atattgagcg gaaattaaat tagttaatta tacaatatac
     7201 aggcgaaagg attctatttc gtctgtatat taatggatga ataccaaaaa gggggatatt
     7261 atgaaaaatc gaatttttgg aaaatatcgt aaagatcaag tgatatgggg ctatattttt
     7321 atttttccaa cggcattagg attaatttta ttaaatatat ggccagcaat tcaaacagca
     7381 attttaagtt ttcaaaaaac ggtagggttc ggtgatactg aatgggtcgg attaagaaat
     7441 taccaaaaaa tgtttgcaga cggtgaagtt tttcagtctt tattaaacac attaatttat
     7501 gcagtggttt cgatacctag tattgttgta ttatccttgt tggtggcagt tttaatgaat
     7561 agaaaagtta aaggattgtc gatttatcgg acaatttatt ttctaccagt cgttgctgca
     7621 ccatccgcag tcgcaatgat ttggcgctgg atgttcaata atgattatgg actgattaat
     7681 aacatgttgg ctaaaatagg tctcgatgga ccagcatggt taacggatcc atcgattgcg
     7741 atttattcta ttatcattgt aggagtttgg agtgctattg gttataacat ggtgttatta
     7801 ctagccggct tataagaaat tcctaaagat tattatgaag cagcggaaat tgatggtgct
     7861 agtccaatcc agcagttttt caacattacg ttaccattag ttacaccaac attatttttt
     7921 gtagttgtaa caacagttat caatgctttc caagtatttg atgttatttt tatgatgatt
     7981 actcctaata gtaccgccat gtttaaatca caatcattag cctatttatt ctataaacat
     8041 tcatttattt taaatgacaa gggatatggt tcggcaatcg ttatgttcct tttagtgatt
     8101 atattaatta ttactgcaat tcaaatgaag ttgcagaaaa agtgggtaaa ctattaagga
     8161 ggctatcaaa ttatgaaaaa taaatttttt tcaaatgcgc caatccatat catgcttatt
     8221 ttaggtggta ttgcaatggc attacctttt atttggatga ttttaacttc agggaaaacg
     8281 ttggttgaat ctacccaaat tccacctaca attttccctg agaaatttca gctaagtaac
     8341 tatcaacaag tacttgattt attaccattc atggcttttt atatcaatac atttttaatg
     8401 gttgttggac gagtgattgg ctcggtttta ttttcagcaa tggctgcata tgcttgtgcg
     8461 cgtctaagtt ttccagggaa aaatatcctt ttcagcttag tacttattca aatgatgatt
     8521 cccggttcca tttttattac tccacagtat ttattagcac aaaaattggg tattttaaat
     8581 acaattccag cattaatatt accaggggta gttagtgcgt ttggtacgtt tttattaaga
     8641 caatttttct taggtttacc tgatgaattg gaggaagcgg cctatttaga aggtgccaat
     8701 atttggacga tctttttcaa aattatgttg ccgcttgcta aatcaggatt ggttgcactt
     8761 ggaatattca cagcgttgtt tgcatttaaa gatttaatgt ggccattgat tgtcaatact
     8821 tcaattgata agatgccttt agctccggga ttagcaaact tacaaggaca atatgccacg
     8881 aacttcccac aattaatggc aggttcgtta ttagcaattt ggccaatagt tgtgatattc
     8941 atcgttttcc aaagaaaatt cattgaagga attgctacaa caggtagtaa attataaaac
     9001 tattaattag tgattggaga gtaagtacat gacaaaaatt acatttattg gtgcaggaag
     9061 tactattttt gcaaaaaacg tattaggcga tgcgatgtta actccaagtc tacaagatgc
     9121 acatattgca ttgtacgata ttgatgaaaa acgactaaaa gaatcaaatt taatgcttaa
     9181 aaatattaat aaaaattcga atgaaaatcg tgctgatatt gtttcttatt atgatcgcaa
     9241 agaagcgtta agagaggctg attttgttat caatgctatt caagtgggtg gttataaacc
     9301 ttcaacagtg attgattttg atattcctaa aaaatatggg ttgcgacaaa ctattggtga
     9361 caccatgggg attggtggga tttttagaac cctacggaca ctaccagtaa tgttagactt
     9421 tgctaaagaa atggaagaag tttgtccaaa tgcttggtta ttgaactata caaatccgat
     9481 ggcgatgtta accatgggtg ttttaaaagc gacgagtatt aagactgttg gtttatgtca
     9541 tagcgttcaa gtatgcgtac cagaattgtt tgagcattta ggaatagccg atcaatatga
     9601 cttaaatgac tttcaatgga aaattgcagg cattaatcat atggcattct tgttggaaat
     9661 tacaaaaaat ggggaagatt tctatccaac aattcgtcag ttagctgcag aaaaaccaaa
     9721 tccacatcca gattctgtac gttttgaatt gttgaaacgt tttggttact atattacaga
     9781 atcaagtgaa cacaatgcag aataccatcc atactttatt aaatcaaaat acccagaatt
     9841 gattgacgaa ttagggattc cgttggatga atacttacgt cgttgtgaac aacaaattaa
     9901 tgattgggaa agcatgcgag aggacattgt aaataatggg gagttaacac atacgagaag
     9961 ccgtgaatat gcttcatata tcatggattc tattacgaca ggaactccgt cagtgattgc
    10021 aggaaatgtc ttaaataaag gcttaatcac aaacttaccg acagatgttt gtgtggaagt
    10081 accttgttta gtcgataaaa atggcgtgca accaacctat gtcggtgaat tgccaacaca
    10141 attagctgct ttaaatcgga cgaatatcaa tgttcaagaa ctgacagtag aagccgcttt
    10201 aacactagat aaatcaaaaa tttatcaagc agcgtattta gatccacatc tatcatctga
    10261 attgtcttta acagatatca aaaatttagt ggatgattta atcgaagcac atggggacta
    10321 tttacctgaa tataattaaa aagaagctga aagagtaagg aattttcaat tccttactct
    10381 tttcttagtt aaatttagta aaaatattga caaaacactt agtaaacagt tacattagtg
    10441 ttaacaaaaa gaaaacgcat tcaaattgta taaggaggag cataaaatga agtggtctaa
    10501 aaaaattatt ttagcttgta tagcactagg agcaacgatc tctttgactg cttgtggaaa
    10561 aaatcaggaa gatgcaagcg gtaaggtaga aatagagttt tttaatcaaa aaaaagaaat
    10621 gactcaaacg attcaggaga ttgctaaaga ttttgaagca aaaaaccctg atattcatgt
    10681 aaaagttgtg gatgtaccga atgctggtga agtaattaaa acacgtatgc ttgcaggcga
    10741 tgtaccagat gtcattaatc tttatcctca aagtatagaa ttgaaagaat gggcgaaagc
    10801 tggttattta gaggacttaa ccgaagaacc gtatttagag aatatcaaaa atggatatgc
    10861 acaacgtttt gctattgagg atcgtgtata tagcatacca ttaaccgcta atgtctatgg
    10921 tttttactat aacaaaactg cttttgagga aatggggatt caagcaccag aaacttgggc
    10981 agaatttgaa aaaatagtag ctgagattaa agatcaaaac aaagtaccgt ttgcgattgc
    11041 aggagcagaa ggctggactt taaatggtta tcaccaacta gctatagcga ctgctgctgg
    11101 tggtgaagag gaagcgaaca atgtttggcg tttctcagaa gttaatggaa ttaatgctga
    11161 aagcaaagaa atgcaaaaag attttaatcg attagatttg ttaagagaac caaaagcttt
    11221 gcaaaacaac tggcaaggtg ccggttataa cgatacagta gttactttta caaaaggtga
    11281 tgcgttgatt atgccgaatg gttcatgggc aatgccgatg attcattcac aaaatccaga
    11341 ttttgaagta ggaacttttc cttttccagc agatgaagca ggtcaaagtc taacgattgg
    11401 cgcaggagac ctagccttat cagtttctgc aacttctcaa cataaagaag aagccaaacg
    11461 ttttgtagaa tatatgacaa cacctgaagc gatgcagaaa tattatgatg tagatggtgc
    11521 accgtgtgct gttgagggcg ttatcgagaa tactcaggat tcaccattaa gtggtttaac
    11581 tgaacttgct tttactgatc gtcatttagt ttggttagcc aaagattgga atagtgaaaa
    11641 tgatttttat accttaacaa caaattattt acacaatggc aatcaaaaag ccatgattaa
    11701 cgcattaaat gctttcttta atccaatgaa agctgacgga aggtaggaaa ataaaatgaa
    11761 aataaaagaa attttaaatc gttactgggc aatcattttt gtagcaattc caattatttt
    11821 gcagatgatt tttttctact ttccaatggt tcaaggtgct ttttacagtt taactaattg
    11881 gactggtctg acttataatt ttgattttgt gggtttgaac aattataaga ttcttttttc
    11941 tgatccgaag tttatcaaat cgattggctt cactttaatt gtgacgcttt gcatgattgc
    12001 tggacaaatt ggttttggta ttttgattgc gcgggcgttg aacgcaaata ttaaagggaa
    12061 aacattcttt agagcatggt tcttttttcc agcggtctta tcgggcgtgg ttgtttcttt
    12121 agtatttaaa caaattttta actatggttt accaaaaatt ggtagtatgt taggcattga
    12181 atttttacaa accagtcttt taggaacaac atggggagcg atttttgcag taatttttgt
    12241 tttactttgg caaggaattg ccatgccagt catcattttc ttagcgggtt ttcaaagtat
    12301 tccacaagaa attattgaag ctgctagaat ggatggggcg aatgataagc aaattttttg
    12361 gaatattgaa atcacctact tattaccaag tatttcaatg gtatttatta tggcattaaa
    12421 aggtggctta acagcatttg atcaaatttt tgctttaact ggtggtggac caagcgatgc
    12481 cacaacatct ttggggttac ttgtttataa ttatgctttt aaaaacaatc agtttggtta
    12541 tgcgaatgct attgctttag tcttatttat cttaattggg attgtttcac tggcacaatt
    12601 aaaattatcg aaaaaatttg aaatctagga ggtttcccaa tgaaaacatc aaaagaaaaa
    12661 aatatttcta aatacttgct tttattatta gggggcttca tgattttagt tcccttagtt
    12721 tcaacgctat tcggttcatt taaaacaaca cccgatatta tgaataattt ctttcgtttt
    12781 cctaatccag cgactttgga aaatttccga agattgttag cggatggaat tggtcattac
    12841 ttttggaatt cggcaatcat cacaatcatt gcagtagttg ctgtgacagc aattattcct
    12901 atggctgctt attcactagc aagaaaaatg actcgtggga aagcatacgg attgatgtat
    12961 ttatttttaa ttttaggaat ttttgttcct ttccaagtaa ttatgattcc gattaccgtt
    13021 atgatgagta aaattggttt agcaaatatg tggggattaa tcttactcta tctcacttat
    13081 gccgtaccac agacactatt tttatatgtt ggttttatta aactaaatgt gcctatcagt
    13141 ctggatgagg ctgctgaaat tgatggggct agtcacttta caacttattt caaaattgtt
    13201 ttcccaatgt taaaaccaat gcatgcgacg actattattc ttaatgctct atggttttgg
    13261 aatgatttca tgctaccatt attaatttta aatcgtgatt ctaatatgtg gacactacca
    13321 ttgtttcaat ataattatac gggacaatat tttaacgact atggtccaag ttttgcttcc
    13381 tatacagtag gaattgtcac gattacacta gtttatcttg ctttccaaag aagtattata
    13441 gctggaatga gcaatggagc agtgaaataa ccaataaatt attctcatta taaaggagtt
    13501 cataaactta tgattatatt tgacaatcac acacaaacat ttcatttaca aaataaaaaa
    13561 attagctatc tattaggaat cgaagaaaat aattatttaa cgcatcttta ttttggaaaa
    13621 aagattacgc actattctgg tgggtatcgc tacccccgaa cagatcgttc cttctcaccc
    13681 aacaatgcaa atgcgcaaga tcgtttatta tctttagaca cattaccatt agaatttcca
    13741 ggatatggac atggtgattt tagagaaccg gcgcataata ttaaattagg aaacggtagt
    13801 cgtgtaaatg attttcgtta tgatagctac gaaattcaaa aaggaaagcc cagcttagcg
    13861 ggattgccag ccttatatac ggaagatgat gatgaagctg aaacgctgat tattcaattg
    13921 aaagacgcga ttagtggatt gactttacag ttgttctaca caatttatca aaatcacagt
    13981 gtaattattc gttctacgca attagtaaat aatagtgatg aaacagtgga gattaaccgc
    14041 ttagctagtc aatcaattga ttttccaaat cgtccactcg agttaattca tttaaatggt
    14101 acctgggcaa gagaacgtca aatgactaga gaaaaaattc atacagggat aaaagtatta
    14161 gatagtaaac gcggatcaag cagccatcaa caaaatccgt ttgttgcttt agtagatgcc
    14221 aaaacagatg aatttcaagg agaggcatat ggatttaatt tagtttatag cggtaatcac
    14281 gaaacggtga ttcaaaaaga tccatttgac caaacacggg tcattatggg aattaattca
    14341 tttaattttg gttggcaact aaaaactggc gactcatttc aaacgccaga agtcgtgatg
    14401 gtttatagta atgaaggtct gaatacgtta tcacagacgt atcatgaatt gtataatcac
    14461 cgtttaattc gtggaaacta tcgtttaaaa gaacgtccca cgttaatcaa caactgggaa
    14521 gcaacgtatt ttgattttaa cgaagaaaaa atcatgaata ttgtcgatga tgcgaagaac
    14581 ttgggcattg aaatgtttgt tttagatgat ggttggtttg gcggtcgtga aaatgacttc
    14641 acttcattag gtgattgggt tgaaacagaa ggcaaacttg cttgtggttt ggaaaattta
    14701 gccaagcaag tgcatgataa cgggatgaaa tttgggattt ggtttgaacc ggaaatgatt
    14761 tctgaagata gtgaactatt tcgagcacat ccagattggg cattgggtat tccaggtcgt
    14821 gaccgctcaa ttagtcgaaa tcaatatgtt cttgattttt caagacaaga tgttcgtgat
    14881 aatatctatc aacaaatgac ggcgattttt gatcgtgtac cgattgatta tgtgaagtgg
    14941 gatatgaatc gaaatatgac agaagtctat tctgtcttgt tggactcaac gatgcaaggt
    15001 gaagtggctc atcgttatat gttagggtta tatgaatttt tagaaaagtt aacaacgaat
    15061 tatccaacta ttttatttga aagttgttcg ggtggaggcg gtcgttttga tgcaggtttc
    15121 ttgtattaca tgccacaaac ttggacgagc gacaataccg atgccgtggc tcgtctgaaa
    15181 attcaatatg gaaccagctt agtgtatcca atttcaagta tgggtgcgca tgtttcagca
    15241 gtcccaaatc atcaaacagg aagagaaacg agtttaacta ttcgtggcaa tgctgcgatg
    15301 gctggtgttt ttggttatga gctagatcta acgcaattaa cttctgaaga aaaagcagaa
    15361 attaaagaac aagttgcctt ttataaaaag catcgccaac ttttgcaata tggaactttt
    15421 tatcgcttgg aaagtccgtt tgaaggcaat gatactgcat ggatgtttgt ctcaaaagac
    15481 caaacagaag ccatggtctt ttatttccga gtattagcag aagctgcgca tccattagtc
    15541 acattgaaat tagcaggatt agcagaagaa gcaacttatc aaatggaaga tgtaacattt
    15601 gctggcgatg aattgatgaa tctcggtttt tatattgact cagaactaca tggtgattat
    15661 gcgacacaac gcttttattt gaaacagata tagtagcttg aatttacaat ataagtgcaa
    15721 caccaaaaga gactcattcc aaacgaagaa aatatgtttt atttacacgc atttactaaa
    15781 aagcagcctg atttgaattg ggaaaatgaa gaagtacgtg aagaaatcta tgcaatgatt
    15841 aactattggt tagataaagg actaggcgga tttagaatcg atgcgatatt aaacatcaaa
    15901 aaaaaactag agtatggcat ctttgaatct gatggagaag atgggttagc cttcattggg
    15961 ccctggattc ttaatcagtc gggcattgaa gtgtggttaa aagaaatgag agagcggaca
    16021 tttaaaccac ataatagtat gacggtagca gaagccgatg ttcctaatga acgtttagat
    16081 gaatatattg gtgaagatgg ctattattcg atggtttttg attttagtta tacggatatt
    16141 gatgtacctg aaactggtga atggtttaaa gatagccaat ggacatggac ggatatgcgt
    16201 accaatattt ttacgaatca attagttacg caagataaag gttggggcgc cttatattta
    16261 gaaaaccacg atcaaccacg ttcaattaat aaatatattc cagaagaatg gatcaatgat
    16321 tatagtaaaa aaatgttggc gacgcttttt atgttactaa gaggtacccc ttttatttat
    16381 caaggacaag aactaggaat gaccaacata gaaatggcat cattagaaga ttttgatgat
    16441 gtagctacgc attctcagta taaacgggca ttagaatatg gattggcacc agagcaagca
    16501 ttgaaagctg tcaataaacg aagtcgggat aattctcgca cacctatgca atggaatacg
    16561 caaaaaaatg ccagattctc aacttctgat aatgtatggt taaaagtaaa tcctaattat
    16621 ccagaactca atgcagaagc tcagttaacc aatcaacagt cggtattcaa ttattatcat
    16681 caattaatta aattaagaaa aaatagtgta tacaaagacg tgctaatcta cggacaattt
    16741 ctaccagtga aagataaaaa tgaacatatt ttgctttatg aacgccaatt agaagataaa
    16801 atcgtattag ttatcttaaa cgttacagca acagaacaag aatattatgt agatgaacag
    16861 taccaacaag tattaatcaa taattatgaa gatgttttat tagatcaaag tcgaaaattg
    16921 cgtttacgac cgtttgaaag tattgtatta gctaattatg gaggacaatt atgaaaatta
    16981 aaaacgaagc aatgctcatc acctatgcag atagtttagg aaaaaacatg ggcgaattaa
    17041 aacaagtctt agacaaacat ttacaaggtg ttattggtgg tgtacatctg ttgccttttt
    17101 ttccatcgac aggtgatcga ggctttgcgc cgagtgatta tacgacagtt gatccagctc
    17161 ttggaacatg ggaagatgtg gaagcattag gtgaagatta ttatttgatg tttgatttta
    17221 tgattaatca catttctcgt gaatcaaaat ttttccaaga tttcaaaaaa aaccatgagc
    17281 agtcaccata caaagaaatg tttatccgta ttcatgaatt tttcccagaa aatcgaccaa
    17341 cacaagaaga tattgattta atctacaagc gtaaagacaa agcgccgttt caaacggtag
    17401 aatttgcgga tggtacgaca gaagaggttt ggaatacctt tggggaagaa caaattgatt
    17461 tagatgtgac caaagaagtt gtgaaagaat ttattcgaga aaccatcaaa gatatggcga
    17521 accatggctg ttctttaatt cgattagatg cgtttgctta tgcgattaaa aaattagata
    17581 ccaatgattt cttcgtggaa ccagatattt gggaactttt agacgaagtg cgtcaagaag
    17641 cagcgaaata tgacgtcgaa ttattaccag aaatccatga gcattattcc attcaaatga
    17701 aaattgccaa tcatgattat tatatctatg acttcgcctt gccaatggtc actctgtatt
    17761 cactttatag tggaaaatca gaacgtttag ccaattggtt gaaaatgagt ccgatgaagc
    17821 agtttacaac attggatacg catgatggaa ttggtgttgt ggacgctcgt gatttattaa
    17881 cagatgaaga attggattac acctctgagg aattgtataa agtcggggcg aatgtgaaga
    17941 aggtctattc aagtgccaat tacaataatt tagatattta tcaaattaac agcacctatt
    18001 atagtgcatt aggcgataat gaccgtagct atctgttagc acgtgcgatt caagtatttg
    18061 cgccagggat tccacaaatt tattacgttg ggttattagc aggaaaaaat gatattgaac
    18121 tgttggaagc aacgaaagaa gggcgtaata ttaatcgtca ttactatgat ttagccgaaa
    18181 ttgaagaaga agtgcaacgg ccagtgattc aacaattatt tgcactttta aaatttagaa
    18241 atgaatctgc ggcgtttgat ttggatggaa cgattgaagt agcaacgcca tcagaatcag
    18301 aaatagtgat tacacgtaaa aataaaaacg gtagtgatag tgcaacatta acggctaact
    18361 tggatacgaa agaatttatc attgagtcta aagatataaa aattttcact agtgttgcat
    18421 taaaatttta acctattcca tagaacaaaa aatcctttat actagcactt ggatgactta
    18481 tccaagacca ataaaaagga ctcatacatg gataagtata aaacaaatac agcatttaat
    18541 aaatggtttt ctgccataaa attaggaaaa ctttctgtca actcccaaaa atgtattttt
    18601 gattacaatt cgtatcgtaa aaaactgtct tttgaagctg tgctcaagct ctttctctac
    18661 gcagttaatg aagaaaaaga aagtttgcgt gatttgagta cagctcttat cgaccgatcg
    18721 ctccaactag aaaccgatgt gtcatcgatt agtcataccc aattgtctcg agcatttaat
    18781 gcacttgaac ctaaagtgtt agaagaaatt tttcaacaac tgttagaaaa agttcaacat
    18841 caaatcaaac caaccaagcg aaatcatctg tatctgattg attccagtac gttttcgctt
    18901 agtttaaaac gtcataagtg ggcgactttc cgtcaaacaa aatcaggtgt caagttacac
    18961 ttaaactatt gttatatgga cgatactacg atgtatccaa ctgattttac cttaacaaat
    19021 gcccatgaac atgatctcaa tcaactacca gtattggtga atcaaccaga ggcaacttac
    19081 gtgtttgatc gtgggtatta ggactttgaa aagatggatc agatgcattg ggatggctat
    19141 ttcttcgtca ctcggattaa aaaaaacacc aaagttcacg tgatagatac tctggaaaca
    19201 tcgccagaaa cagaaattct acgagacgag ctggttcgtt taggatctaa aacctatcta
    19261 accgccaatt tccgactagt cactgttcaa gataagaacg gacgtgtgtt tcaatttatc
    19321 accaatcgaa tggacgtctc ttcaaaagaa atctctgata tgtatcacgc tcgttggcaa
    19381 atcgaattgt ttttcaagca catcaaacaa cacatgacca ttaaaacctt cttttctcaa
    19441 agtgaaaaag gcgtgcagaa tcaattgatt ttaacgatga tttccgctct cctcacgttt
    19501 ctcatcaaat tggagacgaa aacggaaaaa tcagtgttcc aaatcaaacg attctttcgt
    19561 tatttactgt ttcagccctt tgaatgttgt ctagaaaaat taattcccac atgagaaaaa
    19621 agaaatttgg ctcgttactt ttggttttca gttgaataac agataaaaaa ttttctggaa
    19681 aaagtcatcc tctgcgtcag cacagttttt tttgacttat tttcagagga aatagttttc
    19741 agaatattct aaaatgtttt atgcaacact agtgatattt tttagaatta gatttctaat
    19801 gtgcattttt tgagtattaa ggagcaagta tacaaagatt tataaagctg attaaatttg
    19861 aataaaaata tgcaggaacg ctactatttt tagtagtgtt cttttttata tttgaataaa
    19921 aaatgtggtg gttaaatggt ggttataaca aaaaatgaat taatgaaatt aggttatggg
    19981 aagtatcagt ctgagagaat catacgacag gcaaaagcaa ttatggcttc aaaaagctat
    20041 acctattacg aaaatagaag attaggacta gtacctgttg aatctgtaga agaagtttta
    20101 ggtatgaaga tagaggtaga ggaggatttt cagaatcatg gttaatttta aaaaagataa
    20161 aaatgggaaa tatcatttta ctgctagttt gggattcgac aaacgaacag ggaaaagagt
    20221 acaaaaaaac gtagaggggt taaaacagtt aaagaagaaa aaaagcgtat accgaacttg
    20281 tttctaatta tggaaaagaa gcagaaactt ctcaaagtac tatgttgttt gaggagtttt
    20341 tttataaatt attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc
    20401 ggttatctac aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg
    20461 tccaattgac attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt
    20521 aagaaacatg tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact
    20581 tgaaaaaaat cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt
    20641 ttggacaaaa gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca
    20701 acattttggt tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc
    20761 cactgcatta acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac
    20821 actctattat aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat
    20881 tcgtacaatc gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca
    20941 agtttcaata ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata
    21001 tacaatccgt catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat
    21061 acacggatta aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg
    21121 aattaaagaa aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt
    21181 gtatctaaat tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt
    21241 ttctaaaatc aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag
    21301 gatcacacca aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt
    21361 tcttatttcc gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc
    21421 gcattgtcta tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg
    21481 gcagttaata ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt
    21541 ttgtgagggc tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt
    21601 gcaataccgg atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg
    21661 ttttctgtta ctttatatcg ttctttccga atagctagat aattagctaa atattccatt
    21721 gcgaatgctg ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta
    21781 ttcttatcta aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca
    21841 gatcctaaaa tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc
    21901 aaagctcgtt tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt
    21961 gtatccttat ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt
    22021 ttatctgtga tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga
    22081 ggattattgg aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta
    22141 ttgatagtag aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc
    22201 tcttttttta cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca
    22261 taggcagtta actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga
    22321 agccattcat aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga
    22381 tctttatcct cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt
    22441 gtatctattt tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg
    22501 ggagaagagt atctctataa ttggactata ccaattatag aatgttttgt caattggtat
    22561 gctctaaaat ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat
    22621 ttaaaataaa tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt
    22681 gaattttctt aattaattat atagaaagaa caatctttct atagagttag acatgagagg
    22741 gactatgctt gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg
    22801 agaagactat ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa
    22861 ctatttcaaa ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag
    22921 atgaagaaaa acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt
    22981 tagcaatatc tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat
    23041 gagtattcat tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa
    23101 atactctcct aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg
    23161 gcaacttcga gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc
    23221 tatcctactt atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc
    23281 tttcttagct acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa
    23341 agatgctgta aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa
    23401 atatgaagtg atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg
    23461 tgtctttata gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta
    23521 taattcaaat ccaactatat agaaaacgta aattttctat atagttggat taagataacg
    23581 actatttatt ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat
    23641 aatcgcaaat ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt
    23701 tatttgttct tttattaaat acacctacaa cttataaatt gatggtattt agataacacg
    23761 aaagaataaa aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt
    23821 gagttagtgc gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt
    23881 gcagaaatcg ttactgggat taaactaagc aataaaaact aatgttcccc tcattctttg
    23941 aacttctact atttgattta tttattctga taaatgacga tttcaccttc aggatcttca
    24001 aatcccgtat agaatttatt taagatctct tcagtatcag taataggata ggcgataggc
    24061 cacataccct ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag
    24121 catattaaca aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat
    24181 ttcaattctt cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat
    24241 gtgggttgtt gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa
    24301 catcccaaaa ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata
    24361 tggcaccata atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat
    24421 tgatacaatc taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc
    24481 aataaaaacc ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac
    24541 aagaggtctc ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt
    24601 ccattttctt atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa
    24661 tttatatact tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc
    24721 agtgaataga tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg
    24781 attgagaatt tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt
    24841 atataactct cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc
    24901 atttctttca tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa
    24961 attttcagca ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat
    25021 agtttcaaat tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc
    25081 tttattttta tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt
    25141 catctataat ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt
    25201 gactgtctaa ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat
    25261 gttgatcgtc agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac
    25321 tggtttgcat atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta
    25381 ataaaaacta ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca
    25441 caaaatccat tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta
    25501 cagcatcgct ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac
    25561 gatgacacgt tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc
    25621 gaagaaaatt taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt
    25681 aaaggtcaag atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca
    25741 caatgcattt ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta
    25801 atgaaacaga tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat
    25861 tatgttaatc ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg
    25921 tttaaggttg gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt
    25981 aagttggttg acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga
    26041 cagtacctaa aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc
    26101 tcatttcaac ttatatgttt tagtgaacta cccccactta g
//
LOCUS       DAYYNQ010000033        26104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000033 DAYYNQ010000000
VERSION     DAYYNQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.033"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..1082
                     /locus_tag="ACHBJ1_001274"
     CDS             78..1082
                     /locus_tag="ACHBJ1_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430223.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1083..2027
                     /locus_tag="ACHBJ1_001275"
     CDS             1083..2027
                     /locus_tag="ACHBJ1_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430224.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2027..2989
                     /gene="opp4B"
                     /locus_tag="ACHBJ1_001276"
     CDS             2027..2989
                     /gene="opp4B"
                     /locus_tag="ACHBJ1_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM9430225.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3016..3930
                     /locus_tag="ACHBJ1_001277"
     CDS             3016..3930
                     /locus_tag="ACHBJ1_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430226.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3956..5737
                     /locus_tag="ACHBJ1_001278"
     CDS             3956..5737
                     /locus_tag="ACHBJ1_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9430227.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6085..6771
                     /gene="rnc"
                     /locus_tag="ACHBJ1_001279"
     CDS             6085..6771
                     /gene="rnc"
                     /locus_tag="ACHBJ1_001279"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM9430228.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6791..10372
                     /gene="smc"
                     /locus_tag="ACHBJ1_001280"
     CDS             6791..10372
                     /gene="smc"
                     /locus_tag="ACHBJ1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM9430229.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10381..11190
                     /locus_tag="ACHBJ1_001281"
     CDS             10381..11190
                     /locus_tag="ACHBJ1_001281"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9430230.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11201..12199
                     /gene="ftsY"
                     /locus_tag="ACHBJ1_001282"
     CDS             11201..12199
                     /gene="ftsY"
                     /locus_tag="ACHBJ1_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM9430231.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12386..14653
                     /gene="gshAB"
                     /locus_tag="ACHBJ1_001283"
     CDS             12386..14653
                     /gene="gshAB"
                     /locus_tag="ACHBJ1_001283"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM9430232.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14742..15194)
                     /locus_tag="ACHBJ1_001284"
     CDS             complement(14742..15194)
                     /locus_tag="ACHBJ1_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM9430233.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15344..16294
                     /locus_tag="ACHBJ1_001285"
     CDS             15344..16294
                     /locus_tag="ACHBJ1_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9430234.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16287..17246
                     /locus_tag="ACHBJ1_001286"
     CDS             16287..17246
                     /locus_tag="ACHBJ1_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9430235.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17243..17998
                     /locus_tag="ACHBJ1_001287"
     CDS             17243..17998
                     /locus_tag="ACHBJ1_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430236.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18021..18974
                     /locus_tag="ACHBJ1_001288"
     CDS             18021..18974
                     /locus_tag="ACHBJ1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9430237.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19618..19914)
                     /locus_tag="ACHBJ1_001289"
     CDS             complement(19618..19914)
                     /locus_tag="ACHBJ1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM9430238.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20269..20625
                     /locus_tag="ACHBJ1_001290"
     CDS             20269..20625
                     /locus_tag="ACHBJ1_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9430239.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20635..21534
                     /locus_tag="ACHBJ1_001291"
     CDS             20635..21534
                     /locus_tag="ACHBJ1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9430240.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22054..22782
                     /locus_tag="ACHBJ1_001292"
     CDS             22054..22782
                     /locus_tag="ACHBJ1_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9430241.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22927..24954
                     /locus_tag="ACHBJ1_001293"
     CDS             22927..24954
                     /locus_tag="ACHBJ1_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM9430242.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            25062..>26104
                     /locus_tag="ACHBJ1_001294"
     CDS             25062..>26104
                     /locus_tag="ACHBJ1_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM9430243.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLF"
BASE COUNT         8703 a         4563 c         5544 g         7294 t
ORIGIN      
        1 ataaaacgct ttattctgtg taatgtcctt ttttattaag aaaaacttcc atttaggaag
       61 cagagagggg tattcgattg tccgataacc aattattaga agttcagaac ctacatacag
      121 gattccgact caaagatgac tattacgatg cagtagatga cgtttcattt actttagaca
      181 aaaacgagat tttagcaatc gttggggaat ctgggtgcgg gaaaagtacc ttggctacta
      241 cgattatggg attacttgat cctaataaca caaaaattac aggtgagatt ttatataacg
      301 atctgaattt aatcgacctg aacgaaacgc tctataataa aatccgcgga aatgatatcg
      361 gaatgatttt ccaagatccg ttgtctgcat taaatcctct catgagaatc gaagaccaga
      421 tcaaagaagg actttcctat cacacaaaga tgaatgcaga acagcgtcaa gctcgtgcac
      481 tagaattgct agaacaagta gggattccta atcctgcgag agttggacgc caatatcccc
      541 atgaactatc aggcggtatg agacaacgtg tcatcatcgc aatcgcaatc gcctgcaaac
      601 ctccaatcgt cattgcagat gaaccgacga cagccctaga tgtaaccatc caagcacaga
      661 ttcttgattt actgaaagac cttcaggaag aaacaaaaac agggatcatc ttgatcactc
      721 atgacttagg agtcgtagca gaaatggctg atcgagtggc agtgatgtat ggcggacagt
      781 tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc taagcatcct tatacacgct
      841 ctttactgaa ctctatacca caagaaggaa atcatgatgc agaactccat gtgatcgaag
      901 gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg tcggtttgca ccacgtattc
      961 catggatacc agcaagcgat catgaagaag agcctatcct tcatgaagtc gcaccgaacc
     1021 acttggtacg ttgctcctgc tataagcatt tccactttag agacgaaaaa ggagaggtgt
     1081 agatgacaga attaatcaca atcaaagacc tgaaagttca ttaccctatt cgcagtggat
     1141 tcttcaatcg agtaacggat catgtatatg ctgtagatgg tgtggatttt attatcgaaa
     1201 aaggaaagac atatggttta gtgggagagt ctggctctgg aaaatctact acgggaaaag
     1261 ccgttgtggg attagaaaag gtaacagccg gacaaatcat gtatgaagga aaagatgtaa
     1321 cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga tgtgcagatg atcttccaag
     1381 attcaatgtc cagtttgaat cctaaaaagc gagtgttgga tatcattgct gaacccatcc
     1441 gaaattttga acggttaagc gatcaagaag aaaagaaaaa ggtaaaagga ctgctagata
     1501 tcgttgggat gccggaagat gcgctatata aatatcctca tgaattttca ggcggacaac
     1561 gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc aaaactgatc gtagccgatg
     1621 aacctgtatc tgctctggat ctatctgttc aagcgcaagt attgaatttc atgaaacgaa
     1681 tccagcaaga atacggcctc agctatttat tcatttcaca tgatttaggt gtagtaaaac
     1741 acatgtgcga caatatcgca attatgtaca aaggtcgatt tgtagaaatc ggtacaagag
     1801 aagacattta taatgacccg cgacatatct atacgaaacg tctgctttca gctattccgc
     1861 aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg acgaagagta gaacaagagt
     1921 atatccaaaa ccaaaaagaa tactatgatg cgactggtcg agtctacgat ttgcgtacga
     1981 tcacaactac acacaaagtg gcgttgaaag atggaggtgc aagctaatgt ggaaaacgat
     2041 tctgcgacgt gttttattga tgatcccgca agttattatt ttaagtgttc tgatcttctt
     2101 attagcccaa gcgatgccgg gggatccatt taccggtttg atcaatccaa atcaggatcc
     2161 ggcagtgatc gaacagatgc gacaagcagc tggcttgaat gacccatggt atgtccagta
     2221 cttccgttgg atcggaaatg ctttgcaagg cgattttggt caaagtttct tatacaaacg
     2281 ctctgttgct gacttgatcg gcgaacggat catcaacact ttatatctat cgatcttgac
     2341 agttgtcctt acttatttga ttgctgtgcc tctggggatg cttgctggcc ggtatcaaaa
     2401 ttcaatcttg gataagattg tcgtaatcta caacttcttt agttttgctg ttcctttatt
     2461 tatcttcggc ttgatcatgc tatttgtttt tggttatcgt ttaggctggt tcccaacaag
     2521 tggatcacaa acactaggtt tgcaaggtgg atttatcact caatggatag accgtttgaa
     2581 atatattctg ttaccatcaa ttacccaagc atttttggcg actgccgtca caatccaata
     2641 tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt gtcagaacag cccgttccaa
     2701 aggggtaccg acgaataaag tattcagtcg acatattttc cgaaatgcag cgttgccgat
     2761 ggcttctcaa atggggtatg aaatcacttc gttgattgct ggttctgtag ttatcgaaaa
     2821 gatttttggt tatccgggag tcggaaaact atttattgat tccatcggcc aacgggacta
     2881 taccgtgatc acatccttag tattgatttt aggaatcgcg acgctagtgg ggacattact
     2941 ttcagatatc atcatgagta tcgttgaccc gcgtattcgg attcaataaa accaatcaga
     3001 aaggagaatg gatcgatgag tgaaaaaaac aatacagcag ttcaagaaag tattcctcct
     3061 atggggatac gaatgattgc tcgagaattt aaaaaagata aaattgctat tttttcatta
     3121 ggtttgctag tggttttatt attagtgatc tttatcggtg cgatgctgac cgatcaagac
     3181 aaagtgatgt atgttagtat cttcgacaaa tacgctgcac caggaacagt ttcgacacaa
     3241 ggacaaaagt tcattctggg agcagatgaa ggcggacgtg atgtattagg acaattgatc
     3301 ataggtgcta gaaactctgt tacgatcgga tttgcgatca cgatccttac ttcgatcatc
     3361 ggggttggat tagggatcat ctctggctac tacggaggga tcattgacaa catcttgatg
     3421 cggatcgttg attttatcat gattctaccg atcatgctga tcattatcgt cttcgtatcg
     3481 attatttcca gatacaatgt ctggtccttt atattcatca tgagtgcatt ttattgggta
     3541 gctaaagccc gattattccg aagcaagaca ttatctgaag caagaagaga ttatgtcagt
     3601 gcctcaaaaa caatgggaac caatgatttt aaaatcatgt tccgcgaaat catgccgaat
     3661 ctaagttcat taatcattac taatttgacg atgaactttg cggcaaacat cggtatcgag
     3721 acaactttga ctttcttagg tttcggcttg ccggcaaatg tacctagttt aggtacattg
     3781 atcggttatg caagtaacgg agaagtctta gtgaatcggc aatggatctg gttacccgca
     3841 tcaatcctta ttttagtttt gatgttgagt atcaattatg ttggacaagc attcaagcgt
     3901 tctgcagatg cgcgccaacg gttaggctag tatagaagcg aagggggaaa taggaatgaa
     3961 gaaaacgatt tttggatttg cagcgctgat gtctgctgtc gttcttgcag catgcggggg
     4021 cggaggtaca gatacctctg gaaacaactc tggaggcaac ggcaataaca aagaggaatt
     4081 aacttttcca attgcaacaa aaaataatga tgatgcaatc gaagacggcg aattagaagc
     4141 tgcggtcgca acggacagtc agtttaaagg attgttccag tgggagtttt accaagattc
     4201 atatgatgca gcttttatgg ctccttctca tgaaccgcta tttgcaaacg atgacgattt
     4261 cagaatcaca gacgacggag ctgcaacgct tgatctagat gaagatacga aaaaagcgac
     4321 aatcaaagta aaagacaacg tcaaatggtc agatggtgaa gatgtaacgg cggacgatgt
     4381 gattttccct tacgaaatca ttgggaataa agattacact ggtatccgtt atgacgacac
     4441 tttccgtaac atcgtaggta tggaagaata tcatgatgga agtgccgaca caatctccgg
     4501 aatcaaaaaa atagacgaca aaacagttga aatcgaatat aaagaagtaa acccaagcat
     4561 gcttcaatcg ggtggtggta tctggtcata tgcagcacca aaacatacgt tagaaggtgt
     4621 agcaatcaaa gatatggaat caagcgatca agtacgtaaa aatccagtga cttttggccc
     4681 ttattacatg agtaatatcg ttgcaggaga atcagtagaa tacttgccaa acgagcacta
     4741 ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa tctgtaccga tggcaagtgc
     4801 aactgaagca ttgaactcta aattatatga tatgattttc caaatgccaa cagatactta
     4861 cgatacctat aaagaaattg ctggctatac aaacttaggc cgtgaacaaa cttcttatac
     4921 ttatctagga ttcaagttag gtaaatggaa tgcagataca aagagtgtgg aatacaatcc
     4981 agattcaaaa atggctgata aatcattacg tcaagcaatg ggctatgcat tagataataa
     5041 tgcagtaggt gaacgtttct atgcaggttt gagaagcaat gcgacttcat tgattcctcc
     5101 agtattccag tcataccatg atgatgagat gaaaggattc acacaagatc tagacaaagc
     5161 gaaaaaacta ttagatgatg ctggctacaa agatgtggat gacgatggtt taagagaaga
     5221 caaagatgga aataaactaa caatcaactt tgcttcgatg tccggtgggg aaacagcgca
     5281 accattggca gattactata tccaacaatg gaaagaaatt ggtttggatg tccaatacac
     5341 aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa ttagaaaacg atgatcctga
     5401 aattgatgtt taccaagctg cttggaatac aggaactgat ccaaatccaa gtggactatg
     5461 gggaccaaat gctgcgttca actacactcg ttatgaatca gaagaaaata caaaattgat
     5521 caacgatatc agttcaagca aagcatttga tgaagagtac caaaaagaag catatcaaaa
     5581 atggcaacaa tatgcatttg aagaagcatt tgcgattcca acgttgttcc gtaatgaagt
     5641 attgccagtc aacgaccgtg tcaaagactg ggattggtct tatgatgtac caaacccatg
     5701 ggcaaatgtt tctgttacag cagattcacg ttcataatca ctaaaaatga ccgaaaaagg
     5761 gcgatgtcct ttttcggttt tttgatattt ttctgtttat ttctctaata ttcaggtcaa
     5821 ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa gtccagtttc tcggtattaa
     5881 gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa attttttctt catactcgaa
     5941 actaaacgaa tttttcacaa cttctcgtca atcactagga ttattaaagc tttacttacc
     6001 ttttgaccga accatttcaa atttgacaaa aaaccagtat aataaggaag tatcgaaaaa
     6061 atatccataa aaggaatgtg gaaaatggac aaccagctga caaaagaact aaaagaaaac
     6121 tataacatcg tttttcacga tctaaatttg cttgaacaag cttttaccca ttcatcctat
     6181 gtgaatgagc atcgcaatct gcaattatcg gataatgagc gacttgaatt tttaggagat
     6241 gccgttttag aactgatggt ttctcagtat ttattccgtt tatatcctca tttgccagaa
     6301 ggaaaattga caaaaacacg agctgcgatc gttcgcgaag acagcttgtc gaaatttgcc
     6361 aaagaatgtc actttgatca atatgttttg ttgggcaagg gagaagagaa ttcaggagga
     6421 cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt ttttaggcgc gttatatctt
     6481 gaccagggat tcgacacgac ccatgcattc atcgaaaaag tgatttttcc taaagtcaaa
     6541 gcaggtgctt tttcacatga gatggatcat aaaacaaaac tacaagaagt cttgcaaaaa
     6601 tcaggtgatg tatcaatcga ataccgattg atcaatgaag aaggtccagc tcatgaacga
     6661 gtattttgga tcgaagttta tgtagatgat caattgattg gcactggaca agggaaatcg
     6721 aaaaaattag cagaacaagc tgcggcggaa aatgcattgg cagcactgta aaagtaagtg
     6781 agggaaagct ttgtatttga aacgaattga aatagccggt tttaaatcat ttgcagacaa
     6841 gacagtcatt gattttgaaa atagcgtcac agcagtggtt ggaccaaatg gcagcggaaa
     6901 aagcaatatt acagaggcta tccgctgggt attaggagaa caatctgcta aaagtctgcg
     6961 aggcggaaaa atgccggata tcatctttgc cggttccgat acccgtaagc agctgaatat
     7021 tgcagaagtc acagtgattt tagacaatag tgatcattat ctgccattgg agtacaatga
     7081 gatcagtgta acccgccgtt atcgacggac tggggaaagc gagtttttca tcaataaaca
     7141 aagctgcaga ttaaaagata tccaagaatt gttcttagat tctggattag gaaaagaatc
     7201 gttctcaatc atttctcaag gaaaagtaga agcgattttc agtagtaaac cggaagaccg
     7261 tcgtgggatc tttgaagaag cagctggtgt cttgaaatac aagcagcgga aaaagaaagc
     7321 agaacagaag ctttttgaaa cagaagacaa cctcagccgc gtccaagaca tcatccacga
     7381 attggaagaa cagttaactc ctttggccgc ccagagtgag gcagcaaagg agtttttacg
     7441 gctaaaagaa acactgactc aaaccgatgt ctctttgatg gttgctgaaa tcaagacagc
     7501 aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa ttcaatctag aattaggcaa
     7561 actatctgag tcgatccaag aacaagaatc cattttggcg aagcagcgta aagaaaatgc
     7621 gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta ttagatcttt cggaaaaact
     7681 gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg acaaagcata cacaaaaaag
     7741 ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa aaggttaaac attttgaaaa
     7801 gcttcaagaa agcttgatga aagcagcagc agaaaaagaa acagagatcc aaaaagctga
     7861 agcaaatctc ataaaaacac agcaagaatt agaaaaatac caaaaatcaa cgaaagaatt
     7921 attggcagaa ttaagagatc aatatgttga tttgatgcaa gaacaagcag ctgtaggaaa
     7981 cgaactgaag tatctagaac gtcaatatat acaagaaaca gcaaaaagca agcagacgtt
     8041 agccaagcaa tcggaagtag aagcatcagt agaccgattg atcttacaaa aagaagaatt
     8101 gacacaaaag caggcgcagc taaaatcatc tttaactgaa acaaaagaga agttagaaat
     8161 gatccaacaa aacgggaaaa agttccaaga agcattagca aaagaacagc ctaaaatgta
     8221 tcaattaatg aatcaagtcc agcagctacg tgctcgtaag aaaagccttc aagaaatcca
     8281 agaaaactat tttggttttt accaaggggt tcgattggtc ttgcagcata aacagcagct
     8341 ttcgggtatt gtcggagcag tggctgaact gatcgatgtt cctgcagatt tcacgttagc
     8401 tatcgaagca gctttaggtg gggcagccca gcatgtcatc gttgaaaatg aaaaagatgc
     8461 tcggcaagcc atcacgtatt tgaaacagca aagaggagga agagcaacat tccttccact
     8521 gacgacaatc aaaccaagac aattaccggc tcatatattg acacaagcag cagctgtaga
     8581 agggtttata gggattgcca gtgaacaagt cagttatccc gatcaaatcc aaactgtggt
     8641 tcaaaacctg ctaggaacga tccttttagc caaagactta actagtgcca atgcaatcgc
     8701 gcaaacgatc cgctatcaat atcgagtggt ttccttagaa ggtgatgtga tgaatgccgg
     8761 aggatccatg acaggtggag cgaataaacg aggaaaccaa ggcagtttgt ttgtacaaaa
     8821 tcaagaattg aaacagttga ctagtgaatt tgaagaagca gataaacaac tccaagcaca
     8881 agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt ttagccgaag aacaagaagt
     8941 tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa gaagcgacaa atcagctaca
     9001 aaacatcata aatgaacttg aacgttttga aaaagaaaag caaatctcaa cttttgagac
     9061 acgtgaactg cagcagttta tcgaagatta cgaaaagcaa acgaacgaat tgaaagacaa
     9121 acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa gaaatcaaat cgttaagtca
     9181 agaaagcgac cagatggaag ccagaagagc acaagtacaa agtcaaaaag cacaagaaca
     9241 agcagatctt gctgtcttga aagagcagtt taatcatctg cagatccagc tccgcggtgc
     9301 ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg atagaaaagc agttggcgac
     9361 attgacagca gacttttctg atcatgaagt cacagaagaa agtttggaaa aacaaattaa
     9421 cgaattatct gctcagcgag aaacattaaa agccgagttg gcaaaagcca aagagcagcg
     9481 agatagaagg caaaaggaga tcgataagct tgaagcagtc ttggcagagc gtaatcgtga
     9541 gcaaaaagca cgactgtcgg aacaatccaa attagaagtc caaaaagacc gagctgaaat
     9601 gattttagat aatcatttga cctatctgca gtcagaatat cagctgacat ttgagaaagc
     9661 ttcccaagac tatcaagaaa caaccgatat cgaagacagt agaacaaagg tttcatcctt
     9721 aaaagaacag attgaaaaac tgggaccagt aaacttaaat tcaatcgaac aatacgaaca
     9781 agtcagtgaa agacatacat tcttagcaac acagcgagat gatctgttag cagcgaaaaa
     9841 ccaattgttt gaaacgatgg atgagatgga tgatgaagtg cggacaagat tcaaagaagt
     9901 atttgaagct atccgtcaag aattcaaaat tgtctttcca aatatgtttg gcggcggtaa
     9961 agctgaatta gtactgacgg aaccaactga tttgctgaaa acaggtattg agattgaagt
    10021 acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt tctggaggag aacgagcact
    10081 tacagccatc gcgttgctct tttccattat ccgtgtctgt cctgttccgt tttgtatctt
    10141 agatgaggta gaagcagcgt tagatgaagc caacgtgaaa cgttttggtc gttacttgag
    10201 cgatttccaa gatgacacac aatttatcgt agtcacacac cgcaaaggaa cgatggtcgc
    10261 agcagatgta ctgtacggag tgacgatgca ggaatccggt gtatcaaaaa tcgtttctgt
    10321 acggatggaa gatatcaatg aagaggcgaa aatcaacgaa acgaaagtgt aggaacgaaa
    10381 atgatcaaat tagtagcaat cgatttggac ggtacattat taaatgaaca aaagctgatt
    10441 tcagatgaga ataaacaagc attagcacaa gcaaaacagc aaggggtcaa gatcgttcta
    10501 tgtacaggac gccccttagc tgcgatggcg cattatctgc aagaattagg actggtagat
    10561 gaaggtgact acagcatcac tttcaacggc ggtttggtac aaaaaaatga tactggtgaa
    10621 atcatggaaa agaaagtgat ggaagtatca gatatccaac gcttatacaa actggctcag
    10681 caattggatc tgccactaga tgttttgtct gacaatgtgg ttttacagct gcctagtgcc
    10741 ccaaggaagc aatccttgta caatgtgttg aataagctgc tgcaattcca accagcggat
    10801 ataagcgata ttacagatga atttgtgctt aataaggcag taatcgctta tgaccaagaa
    10861 gaattagatc ctaaaatcaa agaaatacca gcagaattcc atgaaatgta tgagatcatc
    10921 aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg ttacaaaagc ttatggtatt
    10981 tctttattgg caaaagatct tggtttgaaa caagaagaaa tcatggcgat cggtgatgag
    11041 gaaaatgatt taccgatgat ccaatatgct ggactaggcg tagcgatgga gaatgctgtg
    11101 cctttcgtca aagaagcagc agatttcgtg acagccagca atgaagaaaa tggcgtagcc
    11161 aaagtgatcc aaaaatttgt attagagtag ggggagtcaa atgggatttt ttgataaaat
    11221 caagcgtgct tttactggcg aagagaaaac agaagaagtt gaagtacagg agaagtacga
    11281 caagggttta gaaaagacgc gtaaaacatt tggtgaacgg atgaatgagt tgtttgccaa
    11341 ttttcgaaca gtcgatgaag acttttttga agaattagaa gaaacactta tcggtgcaga
    11401 tgtaggtttt gaaacagcga tacgaatcac cgaagcatta cgtcaagaag tgaaactcag
    11461 aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc gagaaacttg tggatctcta
    11521 tgaagcggaa ggaatcaatg aagtaaatga actaaatatc caagaagacg gactaacagt
    11581 tattttgttt gtcggtgtta atggtgtagg gaaaacaaca agtattggta aacttgccca
    11641 tcagttcaaa aacgaaggta aaaaagtatt gatggcagca gcagatacct tccgcgctgg
    11701 agcaatcgac cagttagttg tctggggcga acgcgctggt gttgaagttg ttcgtggcaa
    11761 tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg gaaagagcaa agacagagca
    11821 ggcagatgtt ttactagttg atactgcagg ccgactgcaa aataaagtga acttgatgaa
    11881 cgaattagaa aaaataaaac gagtaatcca gcgggaatta ccagaagcac cccaagaaac
    11941 acttcttgta ttagatgcaa ccactggtca aaacgcgatg gttcaagcaa aacaattcaa
    12001 agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta gatggtacag caaagggcgg
    12061 tatcgtactt gctatccgta atgaattgca tttaccagtg aaattggtcg gtcttggtga
    12121 aggaatcgat gacttggagc cgtttgatcc aaatgatttt gtcgttggat tgttcaaagg
    12181 attactaaaa gaagaataac ccatgtctaa aaagcaggag ctgtgacaaa agttgcagct
    12241 ccttgtttga atgattgttt ttaacatagc tgtgtttttg atgccttcat ttttatactt
    12301 atttgtaagc ggttctcaaa caatttcgtt ctttttctta gactttttga ttataattag
    12361 attcatatga attggaggtc acttaatgat gaattttaag caattattat tgcatgtcaa
    12421 tgcgcgccct tttatcgacc aagctcgatt tggtatagaa cgggaaggac agcgggttga
    12481 tcttgcagga aatctagcaa aaaccgatca tccagcaatc tttggcgatc gatcctatca
    12541 cccctatatc caaacagatt ttagcgaaac acaaacagag atgatcaccc ctgttaccga
    12601 ttctattccc gaattatttc agtatctcgc tgctgtttat gatgtgactg ctcgttctat
    12661 accgaaagaa gagatgatct ggccattaag tatgccacct gccttaccgg aaaaagacga
    12721 agagatcatt attgcaaaat taaaaaattt cgaagatgtc ttgtatcgac gttatttagc
    12781 aaaaaaatac gggaagcgaa aacagatggt aagcggtatt catttcaatt ttgaatttgg
    12841 cgatgagttg ctaagaacat tgttcagcca tcaggaagaa tttcaagatt tttccgaatt
    12901 caaaacagaa ctttatttga aaacagcgag aaactttatg cgctatcgct ggatgatcac
    12961 ttatttattc ggtgcttcac caatgagtga gaagaactac tttttagatg aatcacatcc
    13021 gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc tatacgaatc atccaaatgt
    13081 gaaagtttcc tatgcttcta tgaaacagta tttagcagat atcgagcgaa tgatcgaaga
    13141 agggaaactt tcggaggaga aagaatttta tacaccgctt cgtttccgag gcggaaagaa
    13201 agtcgcagat ttagcaacaa caggtgttcg ttatattgaa ttgcggaata tcgatttgaa
    13261 tccttatgca agattgggta tcaatccaga gcaagttcgg ttcttacaat tattcctgat
    13321 gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa tgggtagcag aaggtacgac
    13381 tcgaaataac aaagtagcac tagaacaacc atcagatcaa acagaatttc atcaagaagg
    13441 tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa ttggattggt tggattctct
    13501 ttatttagta gaggaagcat tgactcaaat ggatcatcct gaacaaacgt tagcagcgaa
    13561 actctatcaa gaagcacagc tatcgagtca gcaagaagtt gctgtggcat tgggacatca
    13621 atattataaa gaaagccatg aacgtcccta tcagttggcc ggttttcgcg aaatggaact
    13681 ttctactcag atatttatgt tcgatgccat ccaaaaaggt gtccaagtca aagtattaga
    13741 tgagtcggat caatttttgc gtctgcaatt ccaagaccat gtagaatatg tgaaaaatgc
    13801 aaacatgaca agtaaggaca gctatatcgt cccacttatc atggaaaaca aaacagtaac
    13861 gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga ggcgcagaat tttcatccat
    13921 ggaagaagcg gtaaaagctt atccgagatt tgccgagcag gcatttgtga tcaaacctaa
    13981 atcaaccaat tacgggttag gtatcacgat tttcaaggaa ggagctagtc tggaagatta
    14041 tcaagcaggt ttagccattg cttttcgtga agacagttcg gttttagtag aagaatttat
    14101 gccgggaacg gaatatcgtt tctttgtgat cgacggagaa gtccaagcca tcatgttgcg
    14161 agtccctgct aatgttattg gtgacagtat ccgaacagtg aaagaacttg ttgaagaaaa
    14221 aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct ttagaattga tccaattagg
    14281 tgaattagaa caattgatgt tgaaagaaca agggttgacg atcgaatcag ttccccaagc
    14341 caatcaaatt gtttatctaa gggagaactc aaatatcagt acaggtggtg attcgatcga
    14401 tatgactgat gaattttctg aagcttacaa aaaaatcgcc gtttctgcgg tagaagcact
    14461 aggagctaag attagtggta tcgatttgat tataccagat aaagaaatcg atcctacaac
    14521 tgataaaaaa gcatatggga tcatcgaagc aaatttcaat ccagccatgc atatgcatgt
    14581 ttatcctttt gcaggaaaag gcagaagatt gacgatgaat gtattgaaat tgttatatcc
    14641 tgaagtattt taaaagaaaa agcttttgac agattgtcct atttgatgtc aatctgatca
    14701 aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt tttaaaataa acgatcccag
    14761 aagctttttt tcttttttgg tttttctttt tcagctggcg aagagttttc ttctgggtat
    14821 tttttttcga tatcaatcac aggttcattt actgcttctt ttgctgttaa gagcaatccg
    14881 aattcctcag gatttgcagt gacgtaatcg ttgactacag tgaaacggct attgtttttc
    14941 gttgctagct ggatatattg gttttggaca gaaaggggta gagccccatt caagagtatg
    15001 gaagcatctg gatgtttttt gagttctttt aaaaagtttt ctttgttttt tggttggatc
    15061 atttcagcaa cagtcatact aagatagcag cgttccctga atgtccctaa atatttatgc
    15121 tgttcttcag ggttcactaa aggtgtacca taaagtccct tatccaaatg gctttttaca
    15181 tcatcttgtg ccataaaaaa taccctcctt cttctctttg tctttcttac gattatagca
    15241 tattcccaat aatacctaaa acatatgaaa aagcggttgc ttttgttcaa cgattcactt
    15301 aaaataagaa tgaaaatcat tctcaattag aaaggtggat tggatgcgct acggacgact
    15361 agtagcatta ttagttattt taagtagtat ttcattgttc atcggagtat cgaatgtaca
    15421 attgtcagaa ctgctccagt tagatgggca aagcttacaa atcttgctag tgactcgttt
    15481 gccacgaacg atttgtttga tccttgtggg agcgaccagt agtatttgtg ggttgatcat
    15541 gcaacatttg acccaaaata aatttgtttc accaacgact gcgggcacga tggatagtgc
    15601 gcgtttaggt attttagtgg ctatgatttt cctacctggt gcatccttga ttgtccgatc
    15661 cttgacagca ttttgctttg cttttgcagg aacattgctt tttttatccc ttacgcgatt
    15721 gttcctccag aaaaatcaat tgatgcttcc gttgattgga gtcatgttag ggaatatcat
    15781 tggttcagtt gctacttttt ttgcttatca attccagttg gtacaaaata tgacttcatg
    15841 gcttcaaggg aattttgcga ctgtgatacg aggaaattat gagttattgt atgctgttgt
    15901 tccattgttg ctcttggttt ggctatttgc ttaccgattt acgatcattg gtctgggcga
    15961 atcgtttgca gtaaatctgg gaatagatta ccagaagatg cagtttttag gcattggctt
    16021 agtatcattg gctagtgcag tcatgttgat catggtagga agcattcctt ttatcggagt
    16081 gatcattccg aatcttgtct ctagaatata tggagatcaa gtgcacaaaa caattggcgt
    16141 aactgcttta gcaggtagtt tgttcttagt attttgcgat cttgtcgctc gcgtgttgat
    16201 ttttccttat gaaataccag tcagtgtgat cgttggaatc atcggtgggg ctatcttcct
    16261 ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa gccatctatt tttattggta
    16321 tgcttgttct tgttttattt gttacaggaa tgtacttgac gatccataca tatggaaact
    16381 ggcattttgc atgtgcattg aggagtaaga aaatttttgc atttatcctt gtctcgctag
    16441 ctgtcagcag tgccacaatc agctttcaga caattacgca taaccagttt ttgactcctt
    16501 cagtattggg acttgagtcg ttatatgtcc ttgtccaaac ggtccttttc ttctttatcg
    16561 gaggagtgac gatgcttcag cagacaggta cagttttttt cttattgaac gttgggctga
    16621 tggtcgggct gagtctattg ttatttttct ttcttttgaa aaaaatgaga gcgaatcttt
    16681 ttttattact gatgtcaggg atgattttag gtacgctttt tggtagtgtc agtacctttt
    16741 tacaagtcat catggatccg aatgaatatg atttgcttca aggcagatta tttgcaagct
    16801 ttggcaatat ccagagtcag tatcttttgc ctgcttcttt gatcattatc ggctgctggt
    16861 acctgttatt tcgatatcga catgtgctag acgttttcca tctcggaaat gaacaagcag
    16921 taaacctcgg gatagacaca gatcgttttg aatttttgat tttatgttgt gtcagtcttt
    16981 tgacagggac atcaacggct cttgtgggtc cagttacttt tttagggttt attgtcgcaa
    17041 atatcagtta tcgtgtcttt tctacttatc gtcatggtca gctatttatc ggaagctttc
    17101 tggtagctat gctgtttctg acaggaggac agctcgcagt agagcagatt ttcggctgga
    17161 atacaacagt gagtgtcgtc gttgaatttg tcggaggaat ttattttgtc acaaaattga
    17221 ttcgcgaaag gaagagtatc ctatgattga tttacaaaag gtcaataaac tgtaccatgg
    17281 aaaatcagtg gttaaagatg tacagttaac gattcctgaa aagaagttga ctgcttttat
    17341 cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg atcagtcgtt tgataccaaa
    17401 agatacaggt gaaatctatt tagaccataa cgaagtaaag gcttggaaat ccaatgaatt
    17461 agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata aatgtccaaa tcaccgtgaa
    17521 agaattagta gaatttggac gatttcctta tacgaaagga aaattgacga caaaagacga
    17581 ggaaatcgtt caggaatcac tggaacacct tgggttagaa gaaatagcgg atcaatcgat
    17641 tgacactctt tcaggcggtc aactccaacg tgcgtatatt gcaatggtgc tagcgcaaga
    17701 cacggattat attttactgg atgaaccttt gaacaatcta gatatgaatt ttgcggttca
    17761 aatcatgcag attttaaaaa atttagtaca agaaatcggg aaaacaattg ttattgttct
    17821 tcatgatatc aactttgctg caagttatgc agacgaaatt gttgcaatga aagatggaca
    17881 gttatttacg caaggaacga cccaagaaat gataaaaaaa gatgtattgg atgaattata
    17941 cgaaatggac atacggattt gtgagattga aggccgcaga ttctgtcttt atttctaaaa
    18001 ctaacattag gagagaagag atgaaaaaat tagtagtagg tatcttgatt ctagcatcat
    18061 ttggtttagc agcatgtgga aatagcaaca acaccagtca ggcagacaca aaaaaaagca
    18121 gcacacaaac tgaaactaca ttgacgataa cagatagtaa tggagaccaa atcgaggtgc
    18181 caaataatcc agaaaaagtc gtcgtattcg acaatggttc tttagatacg atggatgctc
    18241 ttggtgttgg agataaggta gttggagcag ctacaagcag ccttcctgag tatctttcat
    18301 cctataaaaa agtagaatca gcaggtggaa taaaagaacc agacttagaa aaaattaatc
    18361 agcttcagcc agatctaatc atcatttctg gccgacaaag agattttcaa aaagatctaa
    18421 gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa aaatccatgg gaatctttcc
    18481 agcaaaatgt cactgcttta ggggagatct ttggtaagca agaagaagct aaaactcaat
    18541 tagaggaatt atctagtgca atcgatcaga cgaaaaagaa agcagaagct actgacaaaa
    18601 aagcactagt cacacttgtg aatgaagggc aattatcagc ctacggatct ggttctagat
    18661 tcggtttcat ccatgattta tttggctttg aacaagcaga tgatcaaatc gaagcatcca
    18721 ctcacggtca aagtgtttct tatgaatatg tgctggaaaa aaatccggat atcttatttg
    18781 tagtagacag aacaaaagct attggtggag acgacagcaa agatgatatt tctgccaatg
    18841 aactggtagc acaaacgaat gctggtaaaa atcagcagat catctccctt gaaccagatg
    18901 tctggtattt aagcgggggc ggtttagaat caatgaaact gatgatcgaa gatgtgaatc
    18961 aagcatttaa atagcataaa taacagagga ttaaactctc atactatgta acaataatgt
    19021 ttcttacatt cttaaatagc aagagcctat cctcaaagaa gttatcaaaa accatccgta
    19081 aaatcgtatt gatgatttta cggatggttt ttatttattt gagaaatctt ttttcaacgt
    19141 gaattcatta tctacatcat acgggttgat tctagaatgg ggagtggcaa aaaatagtgt
    19201 gatccatttt ttcagcatca agatcgcctc ctttgatttg aatcaaaggt acaaagcgaa
    19261 acttagataa aacttaaatt tagatatttt tattttgtac cgatctcttc tttaaacgat
    19321 gttccatctg ctgcttctcg gcaataagtc cactagacta gaaaatatga gaagctattt
    19381 tctgtccagc gttcttatta ctcaaaacaa atcgcagttg tcacaatctc ttctaaacga
    19441 tgttccatct gctgcttctc ggcaataagc ccactagact agaaaatatg agaagctatt
    19501 ttctgtccag cgttcttatt actcaaagca aatcgcagtt gtcacaatct cttctaaaca
    19561 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
    19621 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
    19681 gggaagctta ctccaccgtt ttagtaattg atagaaataa ctctcttctg ctttgatttt
    19741 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
    19801 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
    19861 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
    19921 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
    19981 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
    20041 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
    20101 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
    20161 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
    20221 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
    20281 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
    20341 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
    20401 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
    20461 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
    20521 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
    20581 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
    20641 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
    20701 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
    20761 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
    20821 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
    20881 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
    20941 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
    21001 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
    21061 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
    21121 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
    21181 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
    21241 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
    21301 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
    21361 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
    21421 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
    21481 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
    21541 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
    21601 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
    21661 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
    21721 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
    21781 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
    21841 tatgacagct gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
    21901 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
    21961 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
    22021 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
    22081 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
    22141 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
    22201 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
    22261 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
    22321 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
    22381 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
    22441 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
    22501 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
    22561 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
    22621 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
    22681 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
    22741 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
    22801 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
    22861 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
    22921 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
    22981 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
    23041 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
    23101 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
    23161 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
    23221 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
    23281 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
    23341 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
    23401 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
    23461 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
    23521 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
    23581 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
    23641 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
    23701 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
    23761 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcgctc ttctcggatt
    23821 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
    23881 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
    23941 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
    24001 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
    24061 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
    24121 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
    24181 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
    24241 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
    24301 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
    24361 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
    24421 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
    24481 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
    24541 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
    24601 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
    24661 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
    24721 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
    24781 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
    24841 ttagattcta cagctcatcg tggaatcaga ataaatattc ctgtagaaaa ccaaagaatc
    24901 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
    24961 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
    25021 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
    25081 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
    25141 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
    25201 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
    25261 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
    25321 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
    25381 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
    25441 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
    25501 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
    25561 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
    25621 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
    25681 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
    25741 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
    25801 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
    25861 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
    25921 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
    25981 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
    26041 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
    26101 ttat
//
LOCUS       DAYYNQ010000034        25785 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000034 DAYYNQ010000000
VERSION     DAYYNQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.034"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..429)
                     /locus_tag="ACHBJ1_001295"
     CDS             complement(<1..429)
                     /locus_tag="ACHBJ1_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430244.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLS"
     gene            complement(440..1108)
                     /locus_tag="ACHBJ1_001296"
     CDS             complement(440..1108)
                     /locus_tag="ACHBJ1_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM9430245.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1238..1822)
                     /locus_tag="ACHBJ1_001297"
     CDS             complement(1238..1822)
                     /locus_tag="ACHBJ1_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430246.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2153..2386)
                     /locus_tag="ACHBJ1_001298"
     CDS             complement(2153..2386)
                     /locus_tag="ACHBJ1_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM9430247.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2401..3351)
                     /locus_tag="ACHBJ1_001299"
     CDS             complement(2401..3351)
                     /locus_tag="ACHBJ1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM9430248.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3348..4190)
                     /locus_tag="ACHBJ1_001300"
     CDS             complement(3348..4190)
                     /locus_tag="ACHBJ1_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFM9430249.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4192..4911)
                     /locus_tag="ACHBJ1_001301"
     CDS             complement(4192..4911)
                     /locus_tag="ACHBJ1_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9430250.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            5459..5761
                     /locus_tag="ACHBJ1_001302"
     CDS             5459..5761
                     /locus_tag="ACHBJ1_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430251.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            5833..7059
                     /locus_tag="ACHBJ1_001303"
     CDS             5833..7059
                     /locus_tag="ACHBJ1_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430252.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(7147..7539)
                     /gene="rpsI"
                     /locus_tag="ACHBJ1_001304"
     CDS             complement(7147..7539)
                     /gene="rpsI"
                     /locus_tag="ACHBJ1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9430253.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(7555..7983)
                     /gene="rplM"
                     /locus_tag="ACHBJ1_001305"
     CDS             complement(7555..7983)
                     /gene="rplM"
                     /locus_tag="ACHBJ1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM9430254.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(8517..8984)
                     /locus_tag="ACHBJ1_001306"
     CDS             complement(8517..8984)
                     /locus_tag="ACHBJ1_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM9430255.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(9104..10642)
                     /locus_tag="ACHBJ1_001307"
     CDS             complement(9104..10642)
                     /locus_tag="ACHBJ1_001307"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM9430256.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            10722..11408
                     /locus_tag="ACHBJ1_001308"
     CDS             10722..11408
                     /locus_tag="ACHBJ1_001308"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM9430257.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(11609..12136)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_001309"
     CDS             complement(11609..12136)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_001309"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9430258.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12220..12897)
                     /gene="pgmB"
                     /locus_tag="ACHBJ1_001310"
     CDS             complement(12220..12897)
                     /gene="pgmB"
                     /locus_tag="ACHBJ1_001310"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9430259.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(12894..14024)
                     /locus_tag="ACHBJ1_001311"
     CDS             complement(12894..14024)
                     /locus_tag="ACHBJ1_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9430260.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(14024..16207)
                     /locus_tag="ACHBJ1_001312"
     CDS             complement(14024..16207)
                     /locus_tag="ACHBJ1_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM9430261.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(16204..17229)
                     /locus_tag="ACHBJ1_001313"
     CDS             complement(16204..17229)
                     /locus_tag="ACHBJ1_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9430262.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(17274..18101)
                     /locus_tag="ACHBJ1_001314"
     CDS             complement(17274..18101)
                     /locus_tag="ACHBJ1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM9430263.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(18098..19165)
                     /locus_tag="ACHBJ1_001315"
     CDS             complement(18098..19165)
                     /locus_tag="ACHBJ1_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM9430264.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(19191..20027)
                     /locus_tag="ACHBJ1_001316"
     CDS             complement(19191..20027)
                     /locus_tag="ACHBJ1_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430265.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(20024..20893)
                     /locus_tag="ACHBJ1_001317"
     CDS             complement(20024..20893)
                     /locus_tag="ACHBJ1_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430266.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(20963..22246)
                     /locus_tag="ACHBJ1_001318"
     CDS             complement(20963..22246)
                     /locus_tag="ACHBJ1_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430267.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(22260..23945)
                     /locus_tag="ACHBJ1_001319"
     CDS             complement(22260..23945)
                     /locus_tag="ACHBJ1_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM9430268.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            24220..25245
                     /locus_tag="ACHBJ1_001320"
     CDS             24220..25245
                     /locus_tag="ACHBJ1_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430269.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         7719 a         5222 c         4351 g         8493 t
ORIGIN      
        1 tgatagcgaa aagatgtttt tatctaatat atttgaaaga tctaataatt cagctaattc
       61 attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg aaaatactaa
      121 ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta cgtagccaat
      181 attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa ataaaatttc
      241 cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact ttaatagtgt
      301 ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt ttattgaaaa
      361 attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa cattgttaaa
      421 ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta acggaaaaca
      481 ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt ttcacaggag
      541 attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata gttgcacgta
      601 taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa gacaagcgaa
      661 tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa gtcggaaaga
      721 atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt ttttctaaag
      781 aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc attacgaccg
      841 gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa aactctatct
      901 tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag atgaaataaa
      961 tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg aaacacccca
     1021 cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata tttaaagtaa
     1081 tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag atcgcactgt
     1141 tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt atcaaaaagc
     1201 ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat tcaatccacc
     1261 caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct tttgaatagc
     1321 tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag tggatttctt
     1381 cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta tctagacaac
     1441 agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga tatttggttc
     1501 cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata taattaataa
     1561 atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata ttttctaagt
     1621 ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta atatgcggct
     1681 gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa gcacccgtag
     1741 ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct tctttttcaa
     1801 aatggcttag tccactttct attaactttt ggtgagttat aatataatct ctggtttcta
     1861 tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga cttgaagttt
     1921 caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga gaaagagcag
     1981 aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa tcattcacct
     2041 tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag gataagtcct
     2101 atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc ttttacttaa
     2161 aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga aatcctatat
     2221 ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct ggactaacac
     2281 ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat aattcctgaa
     2341 ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct accctccaaa
     2401 ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg catagtaact
     2461 atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat agataggaat
     2521 attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt ctacgaataa
     2581 ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat ctggtgttcc
     2641 ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg ctttcgagaa
     2701 atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa ttgaagcaaa
     2761 tttagattta gcttctttgt ctagagaatc caatttttca atatacttat ctagattttc
     2821 tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa tatttttagc
     2881 gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc ctttctcact
     2941 ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt ctttgttttc
     3001 ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag tttccaagtt
     3061 aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt caggcaaagg
     3121 ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa ttttatcttt
     3181 tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt ttaatttttc
     3241 gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga atataaaaag
     3301 agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca taacgatctc
     3361 tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac aaataagaat
     3421 gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc aataaacaat
     3481 ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt tttcaagcta
     3541 tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat taatatagtt
     3601 cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa tagataatga
     3661 taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga agtgattaat
     3721 aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc aattgtaatc
     3781 cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa taaatccgtt
     3841 gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact gaaagtaata
     3901 ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat aatgatagaa
     3961 gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat ataagaaatt
     4021 gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat tcctcttaaa
     4081 ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt aattagagca
     4141 ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat attattcccc
     4201 ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat taaatacaga
     4261 ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat caaaatactt
     4321 ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat gtttcaattt
     4381 ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa atggttcatc
     4441 taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac gttgaaattg
     4501 tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga ccttttccaa
     4561 agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt tcattccggc
     4621 atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat caatatcagc
     4681 tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat tattaatgga
     4741 gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg attttccagc
     4801 accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag aaatctcgtt
     4861 caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca taactttctc
     4921 cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat tttagtcaac
     4981 cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac aaaactattt
     5041 gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt tctcaaatga
     5101 gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga agtggatagc
     5161 gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga ctatgtctgt
     5221 tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag tgtgtctttt
     5281 tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga tcattgacaa
     5341 ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca agtacgccat
     5401 gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac aaaatgccat
     5461 gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg gagaggtgaa
     5521 attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt taggagatga
     5581 ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc taggaatcga
     5641 taataatact ctagataagt ggattcgtat atataatttc ccagtaataa gagttggtgg
     5701 tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta aaataacttg
     5761 aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta ctttttttaa
     5821 ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa tctgagatat
     5881 ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa aacatttcca
     5941 aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga aaaagttttg
     6001 agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa aaaattttac
     6061 gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa caggcctcct
     6121 agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt acctatgttt
     6181 ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga tgaattaacc
     6241 aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg ttccatgttc
     6301 gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat tattcaaagt
     6361 atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg taagcaagca
     6421 ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt taataatcat
     6481 ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg tgatagaaaa
     6541 agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac ggtccgtctt
     6601 aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa agataccgtc
     6661 attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt acaagctgat
     6721 caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac ttatactaaa
     6781 ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg aatcaattca
     6841 attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg tcatacttat
     6901 gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc tttaacacac
     6961 tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga taaggctgtt
     7021 tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt aaatgtgttc
     7081 ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt tgatatataa
     7141 aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac ctggtttttt
     7201 acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag ctaaacggaa
     7261 gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc ctgattgtcc
     7321 agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg tttcagtaac
     7381 agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt cttcaacatc
     7441 ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag caactgcgtt
     7501 tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct cctattagat
     7561 taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt ctgctccacc
     7621 gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa gcataccttt
     7681 tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag cagtgattga
     7741 tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg cttttttacc
     7801 tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat ccaaatgagg
     7861 tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg aaagacgtcc
     7921 caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc ctggtttggc
     7981 catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt gacatacact
     8041 tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta cccgctaatc
     8101 tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt tacttctttt
     8161 ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag aggctgtgac
     8221 aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca actcctcggt
     8281 cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt tcttgtgact
     8341 cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag ctgaccctcg
     8401 gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt tctctaatac
     8461 tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa cagtttttac
     8521 gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg tttttctgtg
     8581 gctgttttga attcgataaa gatatcttgt gttggatcgt cgccttcttc tccagaaact
     8641 tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag tgtttccaaa
     8701 cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt gttatctgca
     8761 aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc ggatacttca
     8821 tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa tgtaaggaaa
     8881 cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg gcttagatct
     8941 gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct tgcctcctaa
     9001 cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca aaatcattta
     9061 cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt tcagaatcag
     9121 ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc ttttgtgtcg
     9181 aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt actccaactt
     9241 gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt ccgatgcttc
     9301 tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg aaaacaagcg
     9361 ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca aacccgcctg
     9421 tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc cgataaaggt
     9481 tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt tgccgctgta
     9541 aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca ccaagtaaat
     9601 agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc gaaagcagtg
     9661 catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt aaaaatgtat
     9721 gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact acccaatgtt
     9781 tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc tgagtagtaa
     9841 taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca ccaaaagcac
     9901 ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat atatcatttg
     9961 gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag gtggaaggaa
    10021 agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct tctgcccatt
    10081 ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg ctgatcagtt
    10141 cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca gtttttttga
    10201 ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc attggatgaa
    10261 tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc agtttttctg
    10321 cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt gttttctctt
    10381 catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga atgattgtgt
    10441 ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga aagatcaatt
    10501 cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga aaataagcgg
    10561 atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg gttccgatat
    10621 ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac aaaaatttag
    10681 tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt tattttatca
    10741 ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg ttaggtcact
    10801 ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct tggtatgaat
    10861 tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc aggtgtcggt
    10921 tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc tggttcttta
    10981 ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct ttgctatttt
    11041 cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt tcttgggcaa
    11101 ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg ctgttaacgt
    11161 ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct ggcgatactc
    11221 tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag atcttgtttc
    11281 ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat aaaaaaaagt
    11341 tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc cttcatctta
    11401 ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat agtgttctct
    11461 taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca ctaaatggtg
    11521 ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct ctgatatacg
    11581 gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt cgacgaagga
    11641 aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt ttcttgtaat
    11701 aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt tttgtcacga
    11761 ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct gcaatatttc
    11821 gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga tcagcgaact
    11881 gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac tgatcataag
    11941 aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt gtgcctgttg
    12001 ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat gtcacagaaa
    12061 tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg cctgcgaaaa
    12121 ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat ttccaaaagg
    12181 aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg atacggcttc
    12241 tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa tcacatattc
    12301 agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac cagccaaagc
    12361 tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg gtacattcaa
    12421 ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata cttgcgtgcc
    12481 gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga ggcgtgcatt
    12541 tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact caagaacagc
    12601 tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa gatatagatt
    12661 gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt ccatttttcc
    12721 aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt gctcgttttc
    12781 tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt cgtaatgata
    12841 acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga ttttcatcga
    12901 ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc tgtgcgatga
    12961 agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg aaggatcgga
    13021 aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac acttccgcaa
    13081 atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg cgcttttcct
    13141 tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc ttgctgtacc
    13201 cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat tttccctgaa
    13261 gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga tgcctcgatt
    13321 acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac tgctaattca
    13381 ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt tccgcataaa
    13441 agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat aggcacgttc
    13501 gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac tttttgatta
    13561 tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc atcgttggta
    13621 ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa ttgtcgtatc
    13681 agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag tgggacatct
    13741 ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga agcgtactgg
    13801 atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt caaccttaaa
    13861 ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag cagatcatat
    13921 tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc ctctaaggct
    13981 gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt cccctccgtt
    14041 ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata ggtaatgttt
    14101 ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc aacctgtgat
    14161 ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt ggagaaatag
    14221 aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct agccatagtg
    14281 ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt cctaaatcaa
    14341 tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata cggcaagcct
    14401 ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt tcatagtaat
    14461 gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat aacagcatga
    14521 caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca taatcaagta
    14581 aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact ggcttactga
    14641 gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa tataacttat
    14701 ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat ttaacagcta
    14761 aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata tattctgtgt
    14821 attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt acagctctgc
    14881 tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct aaaaacgatt
    14941 gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca ctgatatgat
    15001 gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc aatgcagcaa
    15061 atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt gcaccttgat
    15121 agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg tacagcagca
    15181 aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa atttccgtat
    15241 cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct acactaacac
    15301 ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc atcgctaact
    15361 gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa tctgtccaat
    15421 aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct tgggcaatac
    15481 ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc ttctccagcg
    15541 taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg cgattttttg
    15601 cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt ttactttctg
    15661 ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct ttttcaacca
    15721 actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca tctacttggc
    15781 agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga ttattttgat
    15841 cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca atgagcgcat
    15901 agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga actttcatcg
    15961 tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca tctgggagat
    16021 tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct gcaaacattc
    16081 cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa aatccatttc
    16141 caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt ttttcttcta
    16201 ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt ccaccagatt
    16261 ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac cgagcgcccg
    16321 attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg tcttttgtaa
    16381 ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat tagcacctgc
    16441 ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag caaacgaagt
    16501 attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg catcttctac
    16561 tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg taccaatttt
    16621 ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat acatcgctgt
    16681 atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt ctttgtcgat
    16741 aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta cgtaataaat
    16801 atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa aacgatgctg
    16861 gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct ttcttgcttg
    16921 atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt cgagggcagc
    16981 caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat cgggagaaat
    17041 ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac gtcggcaaaa
    17101 gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga catctgatct
    17161 tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta tgattgctac
    17221 tttcttcatt tatttctccc tcctttgctt atttacttat tgttattctt tctctattct
    17281 ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta ttgcaatgat
    17341 tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac catataacct
    17401 tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc gccagatcct
    17461 ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt ttgcgtagtc
    17521 aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc gatgatctgc
    17581 acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa attgatcata
    17641 tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc ttcggctact
    17701 ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt gcttcggggt
    17761 gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat aacgattcct
    17821 ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac accattaaga
    17881 cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc tgtcactact
    17941 gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat taacaactta
    18001 ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt ttctaaaatg
    18061 tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct cctcggactt
    18121 gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg taagcttctg
    18181 cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag taaccgttca
    18241 tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg taatctgggt
    18301 ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt gcttcgcgac
    18361 caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg atcccgccat
    18421 agccgatccc tcttaaagaa gcttgtaatg cggcagcact gccactcgtt tcaataactg
    18481 aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct gctttcatag
    18541 gatctagcgt gtaatcggca ccgtattttt ctgctaattc tctacgctgc gctatgggat
    18601 cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca atttgcccga
    18661 tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct tcccggatac
    18721 cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga tccaaccgat
    18781 caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca cgaatgggtc
    18841 cataagtgca gactaactct cctttttgat actctgtcac caaactgccc gtttcttcga
    18901 tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg cgttggcttc
    18961 cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg ataaatggac
    19021 ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa attactcgga
    19081 tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg gctattcttg
    19141 gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt ttatcctttt
    19201 acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata cataatgatg
    19261 actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta gtctggtgta
    19321 ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga caggaaaatc
    19381 gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac agaaatgatt
    19441 ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct aagaccatcg
    19501 atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt tagcatgaat
    19561 atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt ggtatcatac
    19621 aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc ggaaaacata
    19681 taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag ttttgacaaa
    19741 gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa ggagatcact
    19801 aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat atcctgataa
    19861 tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc ttcttgtgaa
    19921 tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc taatagcaac
    19981 aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc aagtccacct
    20041 tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc gatatagctg
    20101 ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg cctaagatat
    20161 tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag atagatcgta
    20221 aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc ttgaacttct
    20281 gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg actgtttgca
    20341 aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca aacaaaacga
    20401 ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg aaaagctgca
    20461 acctatctac taacagatca ttgacgattc cataactatt attgaacata tatttccaag
    20521 caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa cgcaccgttt
    20581 tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa gctgtactgc
    20641 ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa aattcactat
    20701 cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag ttaggattca
    20761 gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc actaaaacag
    20821 ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca gaatgtttcc
    20881 ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta cttttttcga
    20941 acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct aaagcttgat
    21001 cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca cctgagcttg
    21061 tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact tgtacttcat
    21121 caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact tcattttctt
    21181 tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca ggagacatca
    21241 agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct tcagcagcct
    21301 ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg ccatagacag
    21361 cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc gcttcataaa
    21421 cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc atgaaagcat
    21481 ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag ttttgataaa
    21541 acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca tcatccaaaa
    21601 catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt gttccttcag
    21661 cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt tgagcaattt
    21721 ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat ttttctttgt
    21781 cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt tttccatctt
    21841 ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct tcctcgataa
    21901 cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta gcaaaatcct
    21961 gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg atttttgtat
    22021 tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt tctttttcaa
    22081 aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa gagtgcatga
    22141 attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa gcagtaagaa
    22201 gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt ttccacctct
    22261 tattttatta ttttcagcca catatattca tacggctgga atatcaactt tcgttcttga
    22321 ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg taaaagataa
    22381 cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt cgcttcgcga
    22441 ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt cggatggaag
    22501 gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac tgctgttctt
    22561 aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg ccgattgatt
    22621 tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc cagcacactt
    22681 tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc agctaaaaag
    22741 cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag atacgtagcg
    22801 tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac tcgggtaccc
    22861 tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat acctcgtacc
    22921 ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt ttttttcgat
    22981 gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga acgaatcgct
    23041 tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt ctcgccattc
    23101 ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa taccgttcct
    23161 ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt ttctggcagg
    23221 tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa gcgaataaat
    23281 gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa aacttctgga
    23341 tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg tttttctttc
    23401 ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt cacgctgctt
    23461 gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt caagtttaaa
    23521 tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc gaacattaac
    23581 cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc gttgattgcc
    23641 tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg caagaaatgt
    23701 acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt acgcaaagtt
    23761 ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc ttcttcgtcc
    23821 caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt ttgttcaatc
    23881 atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa aagttttgta
    23941 tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca tattatggaa
    24001 aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa atacaaataa
    24061 gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct catgatcatg
    24121 caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg tactatggaa
    24181 ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac tatttatgac
    24241 attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa taaccagcca
    24301 aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt gaattatgaa
    24361 ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct agcattatct
    24421 catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat tcaatccatt
    24481 tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa aacaccagaa
    24541 gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg aattattatt
    24601 ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatacaa tattcctatt
    24661 gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga cacggataat
    24721 ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg tcatatcggc
    24781 tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg ttaccgtcaa
    24841 tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg cggctttcgc
    24901 ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag accaactgcg
    24961 attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga acaatctgga
    25021 aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt gtcctcccta
    25081 ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat caaagccaca
    25141 gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat cattctttca
    25201 accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag aattataaaa
    25261 gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac tcatccctcg
    25321 ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt agatagctgt
    25381 ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa actattttta
    25441 accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc cttatttcaa
    25501 tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga catgtctact
    25561 ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa aaataaccgc
    25621 tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac ttttgttgat
    25681 tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc gatgttgtta
    25741 agatcctttc ccacttttct tctagttaga caaattattt tagac
//
LOCUS       DAYYNQ010000035        25358 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000035 DAYYNQ010000000
VERSION     DAYYNQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.035"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHBJ1_001321"
     CDS             complement(132..509)
                     /locus_tag="ACHBJ1_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430270.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHBJ1_001322"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHBJ1_001322"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM9430271.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHBJ1_001323"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHBJ1_001323"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM9430272.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHBJ1_001324"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHBJ1_001324"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM9430273.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHBJ1_001325"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHBJ1_001325"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM9430274.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHBJ1_001326"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHBJ1_001326"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM9430275.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHBJ1_001327"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHBJ1_001327"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM9430276.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHBJ1_001328"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHBJ1_001328"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM9430277.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHBJ1_001329"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHBJ1_001329"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM9430278.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHBJ1_001330"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHBJ1_001330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM9430279.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHBJ1_001331"
     CDS             10661..11575
                     /locus_tag="ACHBJ1_001331"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM9430280.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHBJ1_001332"
     CDS             complement(11708..13273)
                     /locus_tag="ACHBJ1_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430281.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHBJ1_001333"
     CDS             complement(13266..14717)
                     /locus_tag="ACHBJ1_001333"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9430282.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHBJ1_001334"
     CDS             complement(14731..15747)
                     /locus_tag="ACHBJ1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430283.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHBJ1_001335"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHBJ1_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9430284.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHBJ1_001336"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHBJ1_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9430285.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHBJ1_001337"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHBJ1_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM9430286.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHBJ1_001338"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHBJ1_001338"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM9430287.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHBJ1_001339"
     CDS             21340..22764
                     /locus_tag="ACHBJ1_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM9430288.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHBJ1_001340"
     CDS             complement(22806..23063)
                     /locus_tag="ACHBJ1_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430289.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHBJ1_001341"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHBJ1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM9430290.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHBJ1_001342"
     CDS             24337..25314
                     /locus_tag="ACHBJ1_001342"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM9430291.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8202 a         4317 c         5695 g         7144 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAYYNQ010000036        25116 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000036 DAYYNQ010000000
VERSION     DAYYNQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.036"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..900)
                     /gene="deoD"
                     /locus_tag="ACHBJ1_001343"
     CDS             complement(193..900)
                     /gene="deoD"
                     /locus_tag="ACHBJ1_001343"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9430292.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(917..1735)
                     /locus_tag="ACHBJ1_001344"
     CDS             complement(917..1735)
                     /locus_tag="ACHBJ1_001344"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM9430293.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1759..2925)
                     /gene="deoB"
                     /locus_tag="ACHBJ1_001345"
     CDS             complement(1759..2925)
                     /gene="deoB"
                     /locus_tag="ACHBJ1_001345"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM9430294.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3024..3980)
                     /locus_tag="ACHBJ1_001346"
     CDS             complement(3024..3980)
                     /locus_tag="ACHBJ1_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430295.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3980..5113)
                     /locus_tag="ACHBJ1_001347"
     CDS             complement(3980..5113)
                     /locus_tag="ACHBJ1_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430296.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5106..6668)
                     /locus_tag="ACHBJ1_001348"
     CDS             complement(5106..6668)
                     /locus_tag="ACHBJ1_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430297.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6880..7962)
                     /locus_tag="ACHBJ1_001349"
     CDS             complement(6880..7962)
                     /locus_tag="ACHBJ1_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9430298.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8028..8426)
                     /locus_tag="ACHBJ1_001350"
     CDS             complement(8028..8426)
                     /locus_tag="ACHBJ1_001350"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM9430299.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8447..9109)
                     /gene="deoC"
                     /locus_tag="ACHBJ1_001351"
     CDS             complement(8447..9109)
                     /gene="deoC"
                     /locus_tag="ACHBJ1_001351"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM9430300.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9155..10456)
                     /locus_tag="ACHBJ1_001352"
     CDS             complement(9155..10456)
                     /locus_tag="ACHBJ1_001352"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM9430301.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10597..11613)
                     /locus_tag="ACHBJ1_001353"
     CDS             complement(10597..11613)
                     /locus_tag="ACHBJ1_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430302.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11921..12526)
                     /locus_tag="ACHBJ1_001354"
     CDS             complement(11921..12526)
                     /locus_tag="ACHBJ1_001354"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430303.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12722..13645
                     /gene="coaA"
                     /locus_tag="ACHBJ1_001355"
     CDS             12722..13645
                     /gene="coaA"
                     /locus_tag="ACHBJ1_001355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM9430304.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13704..15269
                     /gene="guaA"
                     /locus_tag="ACHBJ1_001356"
     CDS             13704..15269
                     /gene="guaA"
                     /locus_tag="ACHBJ1_001356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM9430305.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15760..17391)
                     /locus_tag="ACHBJ1_001357"
     CDS             complement(15760..17391)
                     /locus_tag="ACHBJ1_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM9430306.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17405..19078)
                     /locus_tag="ACHBJ1_001358"
     CDS             complement(17405..19078)
                     /locus_tag="ACHBJ1_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM9430307.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19091..19408)
                     /locus_tag="ACHBJ1_001359"
     CDS             complement(19091..19408)
                     /locus_tag="ACHBJ1_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9430308.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19433..20833)
                     /gene="lacG"
                     /locus_tag="ACHBJ1_001360"
     CDS             complement(19433..20833)
                     /gene="lacG"
                     /locus_tag="ACHBJ1_001360"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM9430309.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21070..21855
                     /locus_tag="ACHBJ1_001361"
     CDS             21070..21855
                     /locus_tag="ACHBJ1_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9430310.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21858..22550
                     /locus_tag="ACHBJ1_001362"
     CDS             21858..22550
                     /locus_tag="ACHBJ1_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9430311.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22824..23195)
                     /locus_tag="ACHBJ1_001363"
     CDS             complement(22824..23195)
                     /locus_tag="ACHBJ1_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM9430312.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23595..23825)
                     /locus_tag="ACHBJ1_001364"
     CDS             complement(23595..23825)
                     /locus_tag="ACHBJ1_001364"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM9430313.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23919..24257)
                     /locus_tag="ACHBJ1_001365"
     CDS             complement(23919..24257)
                     /locus_tag="ACHBJ1_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430314.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT         7325 a         5527 c         4440 g         7824 t
ORIGIN      
        1 aaacatgaaa agctgttttt tgaaattgtt ccttgtgact cagagttaaa cggcagattc
       61 acaacctctt ggtaaacggt gttcaaaagc ttacatttgc aataataagt ccagccaagt
      121 caaaaacatg aggagctatt tttgcttgga tgttcttatt acttaatgca gggcagcttt
      181 ttcatttcct tcttattctg ctatcgctgt ttccaatcct acttcgatca tttcattgaa
      241 cgtagattga cgttctgcag ctgttgtttc ttcaccggtg atcaaatggt cgctaacggt
      301 catcaaactt aatgtctgca catggtattt cgcgcctaag taataaagag ccgctgcttc
      361 catttccact cctaaaacgc caagttcagc taattggaac gtctctgtca aatcatcttt
      421 gtagaaagaa tcttccgaaa gaacatttcc tacatgaacc gtatagccct tttctttagc
      481 tatctcatat gctttcagta atagattgaa atcactgatt ggaggaaaat gaaaggcttg
      541 gaaatttttt tcgatcatcg aagaactggt cgctgcacct tgtgctagta ctaaatcacg
      601 aacatggacg tcttttgaca atgcaccgca tgtacctact cgaatcaatt tctttacacc
      661 atacgactgg atcaattcat gggcatagat ggtggctgat ggcattccca ttcctgttcc
      721 ttgaacagaa atacgttctc ctttatattt gccggtaaag cctaacatac cgcgtacctg
      781 attgtaacaa actggatctt ccaaaaaagt ctccgcaata tatttagctc gcaaaggatc
      841 tcccggaagt aaaatcttat cggcgatctc gccttctttc gcttcaatat gcacactcat
      901 tttattgttc ctcctattac aataatgtca atgtttcttt aaccaatgct ttaaaagatt
      961 gtttgactct ctgagttgtt tctacaactt cttcatggct cagattcgct tgcatacctg
     1021 ctgctaagtt ggtaatacaa gagattccta acacttttaa tccgctgtga gctgctacga
     1081 tgacttctgg aacagtcgac atccctactg catcggctcc tattgtccga gacattcgga
     1141 tctccgcagg cgtttcataa gttggtccag aatagcccat atatacgcct tctttaagat
     1201 ccaaacctag tttttctgct gctgcttttg ctgttgcttg atagtcaggc gtataagctt
     1261 gactcatatc tggaaatcta gggcccattg catcttcatt cggaccgatc aaaggatttt
     1321 caccagtaaa attgatatga tcggtaatca gcatcaaatc tcctggtaca aatgattcgt
     1381 tgacaccacc acatgcatta gtcacaatga ctgaatgggc ccctagtccg gccatcacac
     1441 gtactggata ggtcactgtt tgcatggaat gaccttcata gtagtggaat ctcccttgca
     1501 tggctaacac tttcttgcct gccagagacc cataaactag ctttcctgca tgcccaacta
     1561 ctgtcgaaac aggaaagaac gggatctcat cataagaaat cattacagca tcttcgattt
     1621 catctgctaa ttcacctaat cctgaaccta gaatcaaacc aaaatcaatt tcttttactc
     1681 ctttatcaag gataaactgg gtcgtttctt gtaacatctc gcttaacttg gtcatttgct
     1741 ttctcctatt taaaaagatt attttaataa tttcaagaag ctttcgccat tttcagtcga
     1801 agaaacacta aagttctcag caatcgttgc tgaaatatct gaataatatc cttgaggcaa
     1861 tgccccttgt tctttcattt ttttactgta aaccaataac ggtacatatt ctcgcgtatg
     1921 atcggtacca gtaaacgtcg gatcgtttcc gtgatcggct gtaatcatta gcaagtcgtc
     1981 ttcttccatc gcttcaagca attctggaat tcgtaagtca aattcttcaa tcgcatgcgc
     2041 atagccgact acatcccgac gatgaccgta taatgcatca aaatctacta aattggtaaa
     2101 gctgattcct gaaaaatcgt ttttcataac tttcagcagc tggtcaacgc catccatatt
     2161 gcttttcgtg cggacagatt cagtgattcc ttgtccgttg aaaatatcgt tgatctttcc
     2221 aacagcaatc acgtcctttc ctgcttcttt caaagaatca aggactgtgt gtccgaatgg
     2281 attcaacgca taatcatggc gattgcttgt tcgtgtgaag tctcctggtt cacccacgta
     2341 cggacgtgca atgattcggc caatcatata cggatcatct tttgttatat cacgaacgaa
     2401 ttggcaaatc cgatataatt cttctaaagg aatgatttct tcatgtgccg cgatctgaag
     2461 gacaggatcg gcagaagtat atacaattaa atcgcctgtt ttcatttggt gttcaccgta
     2521 atcgtcaata acagctgttc cgctataagg tttgttacat acgactttac ggccagaaaa
     2581 ttcttcgatt tgtttgatca gatcatcagg gaatccatta ggaaacacac gaaatggagt
     2641 tttgatattc aatcccatga tttcccagtg gcctgtcatc gtatctttcc ctacagaaac
     2701 ttcttctaat ttcgtcgcat agccttgatg atctttcact gattccacac cttcaagagg
     2761 acggatcgtt cctaaaccaa gctgctctaa atgtggaacg ttcaatcctg cttctttcgc
     2821 aatgtgtcct aatgtatcag aaccaacatc accgaactta tcggcatctg gtgcttctcc
     2881 aattcctact gaatccatga cgatcagatg tacgcgttta aacatgatta ttccttcttt
     2941 ctttttattt ttattgcctt taaaacaaaa agggcgagac gaaaaacact tcccgtaacc
     3001 cacccttttt attcgctaaa agattatttt gatttgatat agttcttccc gtttgctttt
     3061 ggaccagaag ctttccctaa gaaaacaacc agcacaatga ttgtcagcaa atatggagca
     3121 atttgcagca atactggcgg aatcgaagaa ataactggta attgtgcgcc aatgatactc
     3181 aagctttgag caaaaccaaa gaacaatgag gcacccattg cccctaaagg attccactta
     3241 ccaaagatca tagcagccat ggagatgaac ccttgtccaa cgatcgtacc agctgaaaag
     3301 ttaccggaaa tcgactgagc aaaaacagca ccgcccatac cgcctaacaa gccagaaatc
     3361 agtacgcctg aataacgcat agcatataca ttgataccta atgtgtcggc agcttgagga
     3421 ttttcaccaa ctgaacggag gcgcaagccg aatttcgtct tgaagatgat cacccaagct
     3481 aggacagcaa tcaaaatagc acaataagca ggtaagaatg tatttttaaa gaaaattggt
     3541 ccaatgaccg gaatatctgc taatcctggg aaagaaaaat accccagatt ctgcgtgatg
     3601 ttatcagttt gacctttacc ataccatgct ttgatcaaga aaacacctaa agcaggtgcc
     3661 atcagattaa ttactgtccc gctgataata tggtcagcac gcaaactgac tgttgctact
     3721 gcgtgaatca gagaaaatag gatacctacg acgccaccca ctaaaatagc gatccatggt
     3781 gtcatagaac caagtgatgg actcatcgtc aagttgaaaa cgatggaact aaaagcaccc
     3841 atgaccatga ttccctctag ccctacgttt acgatcccgc ttcgttcaga aaaagttccg
     3901 cctagtgacg ttagaattag cggtgttgaa taaaccagtg tagaagtaag gatcaaggca
     3961 attaattcga tagtcgacat tatagttcgc ccccttcatg gttttgattc gttggtttcg
     4021 attctattgt cgcaacgatc tcttcttgtt ttttcccttg gaagacttta gccatgacga
     4081 aacgaatcag gaagttgatc ccgacaaaga agataatcaa agcaatcgat acattgacga
     4141 tctcaaacgg aacacctgct cctgtctgca tgccaagtcc accgatcttc aaaatactga
     4201 acaagatcgc tgataaaaga ataccgattg ctgatccaga accaagcaag gaaacagcca
     4261 ttccgtcaaa tccaatagat agcgaagtcg tttgaacaaa gaagttttga tacgttccta
     4321 atccttcaac cacaccgcca agccctgcca aagcgccaga aatgaccatc gacaaaacga
     4381 tcgtacgttt actactcatt cctgcatact cagaagcaaa aggattcaat ccaacagaac
     4441 ggatttcaaa tcctaaagtc gttttcttca tcagcagcca aacgaaaata agcgcgatga
     4501 ttgctaaaaa taatcctgag tttacgcgag agccgccaaa agcttgtgtt aaccatggta
     4561 accgcagtga ggcattttga ctgatcatat tggaagagtc caaacttgag cgatagcttt
     4621 caggcatcac ttcatgtaaa gcaaatgtgc ttacgtataa taagatatag ttcatcatga
     4681 ttgttacgat cacttcactt gttccaaaaa atgctcgaag taaacctgga acagccgctg
     4741 ccatcgcacc agctaaggca cctaagatca cgcataacgg aagcaaaaca acttttggca
     4801 gatctggaaa cgcaagtgcc acccagacac tgacgatcca cccgcaaagt gcttgtccag
     4861 aaagcccaat attgaaaaac ccagctgagt tagctacaga aaaacctaat gctgtaaaaa
     4921 tcaacggcgt tgcttgcctt aacgtttcac ctatccctct ttgcgaacca agggctgcat
     4981 ctagcataga agaataacct tggatgggct gaaaaccaaa agcagccatc aatatcgcgc
     5041 ctaagatcaa tcctaaaaca actgaaagta caggcactaa taaattacgg aatttttcgc
     5101 tgcgatcatt catgtgcgcc agcccccttt tctaactctt ttcgtgcttc ttctaaggta
     5161 tagcccgcca tcaatagacc taattcattt tcagatgttt ctttcggatc aacgataccg
     5221 acaatttctc cttcatggat aacagcaata cgatccgaaa catttaaaat ttcttctaat
     5281 tcgaaactga tcagtaacac tgctttgtat ttgtcccttt gttcgatcag acgtttatga
     5341 ataaattcga tcgcacctac atccagtcca cgagttggat ttgcaacaat caacagatca
     5401 ggatctcggt cgacttcacg agcgataatt gctttttgct gatttccgcc tgataaagct
     5461 tttgcgggaa caagttcatt cgttgttcga acatcatatt cttcgatcaa ttctctggca
     5521 tgttcattca ttactgaata attcagcatg ccgtttttac tgtaaggttt ttgatgatag
     5581 gtttgtagtg cgatattttc agataatgtc atatctagga ctaacccgta tttatgacgg
     5641 tcttctggca cgtgtcctac accatgttca gtgatttttc ggggtttttt attagtgatg
     5701 tcttcccctt ttagcttgat atgtccgctt tctgcctttc gcaaaccagt caaagcttgg
     5761 atcaactccg actgcccgtt tccatcgatt ccagcgatac caagtacttc gccagcacga
     5821 acctctaagt tcaagttttt cacggcttct aatccacgat tttctttcac cactagattt
     5881 tcaatagaca aaacgacttc ttgaggcttc gcttcttttt tcatcgtttt gaatgaaacc
     5941 gttcttccaa ccatcatatc ggctaattgt tgtgaggtta cgtctttaac gttgactgta
     6001 ccgattcctt ttccacggcg gataactgta cagcggtcag ctactgcttt gatttcatct
     6061 aacttatgcg taatcaaaat gatcgacttg ccttctttga ctaattcctt catgatcacg
     6121 attaattcat caatttcttg aggggtcaat acagctgtcg gctcatcaaa aatcagtaca
     6181 tctgctcctc ggtaaagtgt ttttaaaatt tctacccgtt gttccattcc aactgaaata
     6241 tcacgaacgt aagcatctgg gttgactgac aatccatact gttcagaaac tttttggatc
     6301 tcttttcgtg cttttttatg gtcaagcatc cctgcgcgac taggctcgct tcccaacacg
     6361 atgttttctg ttacagtaaa ggcatcaaca agcataaagt gctgatggac catcccaatc
     6421 cctaattggt tggctttggt cggacttgtg atctttactt ctttcccacg catcaaaatt
     6481 ttccctgatg tcggctctaa taatccggaa agcacgttca tcaatgtaga tttgccagca
     6541 ccgttttctc caagcaacgc atgaatttct cctgccttta cttgtaaatt gatgttgtca
     6601 tttgccttga atgttccaaa ttctttggta atattctgca tttctatgac aatattttct
     6661 tgaaccacct ttttttcaca tcctaacgat ttctatctag ggcaacgatt gactaagatt
     6721 cacgcttagt gagcgttctt cttttgaaac aaacattact tcagggagtt gttcccttat
     6781 ccaaactgtc acaaggcagc ttggataaat gaacaattct tgttctaatc aaaccatagc
     6841 cttgtcaaac atgtgtctaa caaggctata gaaatggttt tagttttctt caggagtttc
     6901 tggaactttt acgtcacctg aaatgatttt ttcttttgct tcatcaactg cttttttcgc
     6961 ttcgtcagaa agttgtcctt ctgttagacc aacaccgtct tctttcaatc cataaacagt
     7021 gtgttcgccg ccagggaatt taccgtccgc tgatttttgt gccaaatctt ctactactgt
     7081 tccaactgct ttcagtgtag atgtcaatgt gaagtttttc ttttcgccgt ttaatgtgta
     7141 ttcgccttcg tctgattggt cgcggtcaac accgataacc caaacttttt tgtcgcctga
     7201 ttcgttcaat gattttgctt cttggaatac accgttacct gtaccgcctg aagcatggaa
     7261 gatgatatct gcgttttgtg catacatacc ttgagcaatt gaacgtcctt tatctggagc
     7321 agagaagtca ccagcatatt gattcaatac tttgatttct tttcctaatt ctttcgcacc
     7381 tgcatcaaca ccggctttga atccagcatc aaaacgatcg attacttcac cttttacacc
     7441 gccgataaag ccgactacgt ttgtttcagt cgtataagct gctgcaacac ctgcaagata
     7501 agaagcttcg ttgtctttga aagttgcaga tacaacgtta tctaatccat cgatgacatc
     7561 atcgataata acaaagttcg tacctgtatt gtttgttgct tgttcttgga tcgctggttt
     7621 tagtttataa ccaataccaa aaatcgtttt gaatccagca ttcaatgctt ggtcaatatt
     7681 tgggatgtaa tctgattcat ttgaagattg gaaatattgg aagccatcat ttcctcgtga
     7741 aagtccttga tctttccccc atttttctag tccttcccaa gctgactggt tgaatgaacg
     7801 gtcgtctaca ccacctgtgt ctgtgatcaa tgctgcagtt gttgtgtctg atgagctttt
     7861 gccaccggat gaatcagtag agccgttgtt gccgcatgca cctaaaaata accctgctgc
     7921 tagtgcgaca gcacctaaac caaataattt tgcttttttc atttctgtaa agcctcctaa
     7981 aataattgtt ttatttttca acagtcagct gactgaatga acatgagtta tagatctttt
     8041 tcagtaaatg cataaggcag cagtgcacga acggtcgttg ccttcgtcac accattgtct
     8101 cctactagcg taaccggcat atcaggggaa cagaattctg ccattacttg tcgacatgca
     8161 ccacaaggag agataggatc gggggtgtgc cccgctacga ctagatgcgt aaatgttttt
     8221 tctccttcag atactgcttt gaaaaaagct gtgcgctcgg cacaattcgt taatccaaat
     8281 gatgcattct caatgttgat gccttgataa atcttgcctg attccgttac gagacaagcg
     8341 cctaccggaa aatgggaata aggaacatag gctttatcta acgcttcaat agctgtgttg
     8401 atccattctt gtttgagttc tgccatgagc ttctcctttg aaaaaattaa tagccttcac
     8461 cagttgaacc agatacgatt gctactcctg cgcttgttcc gattcttgta gcaccagctt
     8521 cgatcattgc cattgcttct tcttcgttat ggataccgcc ggaagctttg acacccattt
     8581 ctggtcctac agtttcacgc atcaaacgga catcctctac ctttgctccg cctgttgaga
     8641 aaccagttga tgttttgaca aaatcagcgc ctgcagcttt tgctatttcg caagctcgga
     8701 tgatttcttc tctatctaat aacgatgttt caataataac tttgacaagc gcacgatctt
     8761 ttgctgcttc aacaactgct tcgatatctt tttggacttg ttgatcatcc tttgatttca
     8821 aagcaccgat attgatgacc atatcgactt catctgcacc gttattgatg gcatctgttg
     8881 tttcaaatgc ttttgtctcg ggtgtgtttg ctcctagagg aaacccgatt actgtacata
     8941 cggctactgg ctcaccttgt aattgttctt tcgctagcgc aacccatgct ggattgatgc
     9001 agacagaata aaaatggtat ttttttgctt cttcgataat ttgcatcaca gcttctttag
     9061 atgcgtctgc tttcaaaata gtatggtcga tcattcggtt tagttccatt tcttttcccc
     9121 ttttctttaa aacggtaaag tccgcttttt tcctttattc tgtgatgatg tcatgaatca
     9181 acgttggctt cattgccgat tcaccaattt caatattttt atataataat tcaatgacag
     9241 aagtgatttc ttcgtcgtta ctatagatcg tcaatagtga ctcacctttt gtcacagtgt
     9301 caccaatttt tttatgcagt ttcaatccaa cagcgtgatc aatatcttct tctttggttt
     9361 tacgcccagc acctagcatc attgcagcaa ttcctaattc atttgccacg atttttgaca
     9421 caactccgct ttgtttagca ggaagctcca ttgtgtagcg tgcagtcaaa attcgttctg
     9481 gatgttccac gatcgctggg tctccccctt gattttggat catttctttg aattttgcta
     9541 acgctttacc tgattccaga gcttcttgaa gcaacgttct tgcttcatcg gttgtcttag
     9601 ctttgccagc aagaacaacc atttggcttc ctaaggcgta gcacatttct actagatctt
     9661 ctggaccatt tccttgtaat gtttcgattg cctcgacaac ttctagacta tttccgatag
     9721 cttcgccaag aggttctgac atatctgaga taaccgccac tgtttctcgg ttagccagtt
     9781 tcccaatgct cgtcatggtc tttgccagac gtcttgcatc ttcgatgttc ttcatgaaag
     9841 caccgtctcc agtggtcaca tccaaaacga ttgcatctgc tcctgccgcg atttttttac
     9901 tcataatcga actggcaatc agcggaagag aatcaacggt cgctgtgacg tcacgcaagg
     9961 catacaattt tttgtccgcc ggtgccaagt ttcctgattg accaattacc gctactttac
    10021 tttcattaac aatgcgtaca aattcttcat ccgaaagctc gatctgaaat ccgggaattg
    10081 cttctagctt gtctagtgta ccacctgtgt aacctagacc gcgtccagac attttagcca
    10141 ctgtcactcc gacactagct actagggggg ctaaaatcaa tgtggtcgtg tcaccaacgc
    10201 cacctgtcga atgcttatct acctttattc cttcaagaga actcaaatcg atgacatcac
    10261 cagaattagc aatcgccaat gttaagttcg tgatttcttc atttgtcatg tcttgataaa
    10321 aaattgccat caataatgca ctgacttgat aatctggaat ctttccattc gtatagttcg
    10381 tgacgatgta ctcgatttcc tctttcgaca gctcgtttcc atccctcttt ttttcaatca
    10441 gatccaccat tcgcattgcc tgatcctcct tgtcttcatt cggtattata cattattttt
    10501 aggaaaggta aaccacttta gtaaactggt ttactaaaaa taaaaaaagc actaactagc
    10561 ctttgtaaat gcttacataa gcaatatacc gccgagttag ccctttgtca atacttttac
    10621 gctctcacga ataattaatt ttgtttcgaa cactttgttc ggaacttttc tttgaggaaa
    10681 ttcgatcgta tcgatcagga aacgtgcagc ataaaatcca atatcaaaac ttggctgctg
    10741 cactgtcgtc aattgcggaa tcatgtattc tgacagcttt aatccatcga atcctatgac
    10801 tgagatatct tctgggattt ttttgcccaa ttcatcgatc gcctgatagc atccgatcgc
    10861 catggcatca ttcccgcaaa aaatagccgt gatatcttta tgtttgagta atttttttga
    10921 tgcagtatat ccaccttgga cagtcaactc tccatgttca acaaatttcc cattaaaagg
    10981 caggccatga tctttcagcg catgacgata tccgttgaaa cgttcttcta attgatagta
    11041 tccagtggtt tccttcagca tgccgatctt cgtatggcca ttttcaataa acagcgaaac
    11101 agcttgatag gcgccctcat attcctttac gataagccgt ccgttatccc gacgattcaa
    11161 tccgcgatcg attaaaatga ttggcatttt ttgataaaaa ccgctgtcta atgcatattt
    11221 ctcaggtaaa atatttggtg tggccagcaa gattccgtcc actgagcggt gggaaagctc
    11281 ttgtaaatac ttgatctcat tttcttggct atgacgagaa ttgcataaca tgatcacata
    11341 gcccaaagga ttcaaatagc tctctacacc ttcgatcagt ttggagaaga agaaatcagt
    11401 gacatcaggg acgatcattc cgatggtctt tgaatgtcga ttgatcacat ttgaagcgaa
    11461 ataatcgggc ttataatgat gttcattcac gatcgacaac actttttttc ttgtcttatc
    11521 actaaatctg cttcctttgt tattcaaaat ctgtgagaca gttgttactg atacgcctgc
    11581 catttctgcg atatctctga tggtcagctt catcctattg gctcccttct atctaaattc
    11641 cctatcagtg taccaaaacc cccaagaaaa gaatagagaa aaaaggaaag tatgtttttt
    11701 gataaaaacc tcctttatct accgacttgg cagccttcta tacaagttct gtatcttatt
    11761 tattttatcg taaaaaacaa cgactctgtt cctactattt ctttttgaag aaaaaatagt
    11821 tctttgtttt taaaaacttc tcaacaaaaa aagaactgat acgtacccgt acatgcacgc
    11881 ccagttccac tctttcgtcg ctccactatt tttcagctag ttattctttc acacttttga
    11941 tgatataata tcctttatct tttgtgacga tttctgcatt accaaatgtt tcttccattt
    12001 tcttttgcgc actcggcgct ccttgtttct tctggatcac aattgtcagt gtaccgccct
    12061 tcttcaaccg aggatatgcg cctgtcaaaa tttcatgtac aacttttttt ccagcacgga
    12121 tcggcggatt gcttacgata gctgcatagg tttcttcatg aactgcttca tagatattcg
    12181 attgatggat atcaacatgt tcgatttggt tacgctttgc gtttccttgc gctagactta
    12241 ccgctcgttc attaatgtcg accatttcaa caaaccgctg tgtcgcaaat gccaaagcta
    12301 acccaatcgg tccatacccg catcccacat ctaagatctt tccttcatca ggtaactctt
    12361 cccaatcgaa agcatcaatc aggacacgtg aaccataatc cactgtttca cgtgaaaata
    12421 caccagaatc tgttacaaat tgaaaggttc tgccttttaa ctcaaaagcc cattgttcaa
    12481 aatcgtgcgc cgtatctggg cgttcagaat agtaatgatt tgtcatgtct tccatccttc
    12541 tcatttcttt catgacaaca gtctatcctt ttatgtatcc gtgcgcaagt caagaataag
    12601 actccgtttc tttctattat aaagattata caaaaagatc ggcaatctct cttaagtggt
    12661 ggggtttgcg tttattttgt gttaaaatag ttccattgat gggagaacgc aggaggctaa
    12721 tatgaatgat ccaatgaact actaccgtgt ggcaagagaa gaatggcgag aattttatcg
    12781 taatgggcaa gcacctttga cacaagatga actagataat atcaaaagtt tgaatgaccg
    12841 catttccatg caggatgttc gagatatcta tgtacctctt gctcatctga tttatcttta
    12901 tatgaaagaa tttgaatctt tgttcttgag taaaggttta tttcttcata aatacgtaga
    12961 agtaccgcct tttattatcg ggatcgccgg aagtgtagcc gttggaaaaa gtacaacagc
    13021 cagactgctg cagacgattt tgtctcgtac cttcaaacga agaaacgttc agctgatcac
    13081 gactgacgga tttctttatc caaatcgtat attaaaagag cgtggaatca tggaccgtaa
    13141 aggatttcct gaaagttatg atatggagat gctgatcgat ttcttgaacc gtgtaaaatc
    13201 tggtcaggaa gagataaaag tacctgttta ctcacatgat gtgtatgata tcattccaga
    13261 caagtacgaa ttgatccagc agcctgatat cttgatcgtt gaggggatca atacgcttca
    13321 attacctgcg aatcagcaaa tctacgtcag cgactttttt gatttttctg tctatgtcga
    13381 tgccgatccc gtgctgatcg aaaaatggta tttggatcgt tttggtgcgt tactggatac
    13441 ggctttccaa aatccagaga attattatta tcgttttgct atcggagatc gtgaagaagc
    13501 ctttaaaatg gcaaaagatg tatggaaaaa tgtgaatttg aaaaatttac aggaatatat
    13561 cttgcctaca cgaggaagag ccgatataat tctccataaa actgaaaatc atatcatcga
    13621 ttcgattttc atgcgaaaat attagcattt taaaatagtt tgactatttt gagataaaat
    13681 ttaaagggat aggggtaata aatttgacaa acgttgccga tatgacgaac gttgaaaaga
    13741 ttatcgtatt agactacggt agtcagtata accaattgat cacaagacgt attcgtgatc
    13801 ttggagtctt ttccgaatta ttgagccacc gcattactgc agaagaagta aaagcgatga
    13861 atccaaaggg aatcattctt tctggcggac caaacagtgt atatgatgag aatgcatttg
    13921 ggatcgatcc tgagatcttc aatttaggta ttcctgtatt agggatttgc tatggtatgc
    13981 aattgatcac gtacaaactt ggcggttccg tagagcctgc taaaaaccgc gaatatggga
    14041 aagccttgtt agaagtaaca agcgatcaag ctgtcttgtt taaagaaaca ccaaaacaac
    14101 aaacagtatg gatgagccat ggtgacttgg taactaaagt tcctgaaggc tttgaaacag
    14161 tcggaacaag caaagattgt ccgattgctt ctatccaaga cacaacacgt aatttttatg
    14221 gtatccaatt ccatgcagaa gtacgtcatt ccgaatacgg aatcgaattg ctgcgccact
    14281 ttgcctttga tgtttgtcac tgtgaaggta actggacaat ggataacttt atcgatatgc
    14341 aagtagaaaa aatccgtgaa caagttggtg acaaaaaagt cttgcttggt ctttcaggtg
    14401 gcgttgattc tagtgtcgtt ggggttcttt tacaaaaagc gatcggtgac caattgacat
    14461 ctatctttgt tgaccacggt ctgctacgca aaggcgaagc ggaacaagta atggaaagtc
    14521 taggcggaaa attcggcttg aacatcatca aagtcaatgc aagagatcgc ttcttagaca
    14581 agttgaaagg tgtatctgac ccagaacaaa aacgtaaaat cattgggaat gaattcgtat
    14641 atgtttttga tgacgaagca acaaaacttg ctggtgaaga aggcgtctct ttcctagcac
    14701 aaggaacgtt gtatactgac gttatcgaat ctggtacaga aacagcacag acgatcaaat
    14761 ctcaccataa tgtaggtgga ctgccagaag atatgcaatt tgaattgatc gaaccattaa
    14821 acacattatt caaagacgaa gtccgtgaat tagggactca gttaggcatg cctgattcaa
    14881 tcgtttggcg tcagccattc cctggtcctg gtcttggtat tcgtgttctt ggcgaaatca
    14941 ctgaagacaa gctagaaatc gtacgcgaat ctgatgctat cttgcgtgaa gaaatcgcag
    15001 ctgctggact tgaccgtgat atctggcaat atttcactgt tcttccaggg atccgctctg
    15061 tcggtgttat gggtgacgga cgtacttacg actacacagt cggtatccgt gctgtaactt
    15121 caatcgacgg aatgaccgct gactttgccc gtatcccatg ggaagtcttg caaaaaatct
    15181 ctgtacgtat cgtaaacgaa gtagcccacg tcaaccgtat cgtgtatgac atcacttcta
    15241 aaccaccagc aacagtggag tgggaataaa gagccaacat aatatggagg gggaatccct
    15301 ataaatcaac attcttcaat agctaaaatg cttgattttc attaaaaata agatggttcc
    15361 ctaccaaaag agactataat ataagttaat ttgtttcatt cttatcataa aaagaagttc
    15421 tcgaaaaacg aactgttttc cgagaacttt tttctatcca tctaagtgct caaatcatat
    15481 tcacgacgga attctaaggt taccctttct taaacaaact ttatgttatc tgcacaactt
    15541 tttgcaattt tttcattgct tcttacgaaa tcgttgaact ctctttagca taatacaaag
    15601 tgtagatgaa gtctggtgct ttactcgttt tggtaaaaat gactggaaat cgtttctggt
    15661 tatttttggc aaaggcgagg tgcaggttcg attcctgcct tctacgctaa cgatgtggct
    15721 gaagggaacc tccgatcttg aatcgaaacg atccgcagtc tattgtttca ccaatgaaac
    15781 aaaatcaacg ttcgtccaag catttcctga agccttgcta taaaccccaa tttcacaaac
    15841 acctgttgta attggaatgt ctttgattgt aatttttgtc cattcacttg ttttaacaac
    15901 cgcagtattt agttccgctc caccataatt tttgatatat aactgattag tatcaaactc
    15961 tcccccagac attacatagg cactcaatgt gtaagttcct ttgcttatgc cactaacatt
    16021 ttgataagct gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt agtccccaaa
    16081 aaaagtgccc gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa accaggtttg
    16141 ccaattggaa ggactggtcg ttggtccatc gctttcgaaa cttgcatttt taactagatt
    16201 tatttgcgcc cctgcttgtt taaacgcacg caagcttggc aaggcgtttc cagcaaaatc
    16261 aaacatcgct tggttgtccc attcgtttcc catagtggat tgaacactga gataattcat
    16321 acctgcttgt gttgcccaac tcacatttcc gttgtaccaa agaggttccc aatagaagaa
    16381 gcccgccccg cgattattcg gtactgcctg aatccgatcg gcaagatcca gcaaatactc
    16441 tgcttgtcct gtcggtgttg gttcatatcc ccccgcagct gcttcggctt caccaaaagc
    16501 atttggcata gtatcggcat tcgctgttgt atgcccataa gctgtttcaa ctacgatcac
    16561 ttctttacca taccgttggc tgatcgcatt catattggtt tgaagttcat tcattgaacc
    16621 gtgccaatac ggataatacg acactccaat taaatcaaat gacacacctc gactcgtgat
    16681 ttcatcaaac caccaagtaa atgctcctgt atcgccacca tcagctaagt ggagcattac
    16741 tggagttgtt ttaggttgag tgtctcgaac agcttggata ccagacttca aaaaagcagc
    16801 caatcgatca aattctcccc cgccttctcc ccaagacttg ccataaggcc agagcattcc
    16861 gctattcagt tcattgccga tctgaaccat atcaggataa acgtccagct gcttcatttc
    16921 cgacagaacg ttctttgtat agtcgtacaa tgccgtattc atttcttcaa aggtcagacc
    16981 ttgccatgct ttaggaagat tctgtttccc aggatcaacc caaaaatcac tgtagtgaaa
    17041 atccagcagc accttaagtc ccagattttt tgctcgtttt gcaaggtcaa tcgtcgtttc
    17101 cagatcattt gtccctgctc cataactttc tccattacta ttataaggat cctgccacaa
    17161 acgcagtcga acatagttga caccattatt ttttaaaatc tctaaggcat cttttgaaat
    17221 atcgtcttca taatattttg caccagattt ttccatatct gacaggatcg aaatatctgc
    17281 tccgcgaata aattcgctgg cagatgcttc aacagttttt gaaacagccc ccaaagcccc
    17341 taacacagct gcaaataaaa cagctttacg caaccaaatt cccattttca ttatggctcc
    17401 tcctttattg aaattggttc agaataaact gaacagcttt ttcaggatct ctcgttaatc
    17461 cgatatattc tgctcctttt gtcgcaacca gcttgattcc taagcgatcg gtgtcttctt
    17521 tgatatcatc atagtaagaa ttgacttgcg gtgctaagac caccatatca aaatcctgca
    17581 gcatttcgta atgagaacca taagcacccg ccattgcact gatcggtgct ttcgattcag
    17641 atgcaccttt ttttaaagca ttagctaaca tcccacttgt ccctgcgcca gcacaaagta
    17701 ccaacacatt gatttcttta tttgcatcgc tgataacttg actcgctgcc acaggtactt
    17761 cctcacgtac atcggcagaa gcaccttcct cggttgcgat cacctctgct ttttgtgctt
    17821 ctttctctac taagacagca tcgtatgctt tgcaaaatgg tagatagatc aggaagtcaa
    17881 cgacgattag aaccgctgct agaacaaaag ctaagatacc catgcccgtt cctagaacca
    17941 aaccgatcgg tgctggagtt gcccaaggca aaacgtacat aaagctgttc atgcccaata
    18001 catcgacaaa aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa aatggaataa
    18061 aaaaataagg gttcaaaatc atgggtgccg caaatagcaa tggttcgttg acagcgaaac
    18121 ttactggaat aaaggaagct ttcccaactg ccttcaactg cttagactta gcaaagagta
    18181 ggaagataaa aggaacaacc aatgttgccc cagttcctcc catagttcct acaaaattcc
    18241 ctaaacccac cgtcagtact ttagaagctt gttcaccgga attgaacaat tgcagattgg
    18301 tttccacatt ggcatagata attgccgcaa ttgccggctc cacgatcgac ggtccatgaa
    18361 caccgacaaa ccaaaacagc gccatcgctc cccagataat agcgattcct aagtacccgt
    18421 cagctgctgt aaatagcggc tgcagcaccg taatgattgc ttctgcaaat gagatttgga
    18481 agatttgtcg aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa gagaaaggaa
    18541 aaatatctcg gaaggtttgc gaaatcgttc caggaacttc tttaggcatt ttaatggtaa
    18601 tatctttcag cacacaaaac ttatataaat taactgttaa aaatgcagcg acaaaggaag
    18661 ccagtagtcc ctttgtcccc atatattccg ttgaaaatcc accttcaatc ggagagacgc
    18721 ttaacaacag gaaacttacc acagaggcca gcatcacaga ggtggtgttg attactttgt
    18781 ttttcggtaa gcgacgattc atcgaatcag ccaagcagcg agcagtggtt gctgaaacga
    18841 gtaaccctac aacacccatc gaataattat agactttcca tagccatgtg gagacagatt
    18901 caggccattg gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa atgcttgaaa
    18961 ataaaatagc tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa taactattac
    19021 gtgagatttt ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt ccatccatag
    19081 cgttcttctc ctatccttga ttctgtctgt acagtacaat aaagtcttga atcagatctc
    19141 tcagcaataa agtggtcatt aaatgatcct gcccatggat aaaaatgaat cccatctcca
    19201 tctctttccc agatgcttct tccgctaaaa tttttgtttg cgaattatgt gcaagggtta
    19261 aattttcgtc ggcttctttc aacgccgaat caacatgttc aaagtttcct tgtcttactt
    19321 ctttcaataa tttcaataat gtactgcgag cgtcgcctga atatgcaacg atctcaaaac
    19381 caagcatttg taattcctct ttattcatct tttcttcctc cattatcact tattaaataa
    19441 tcgttttggt ttcactgacc aacttgtacc agtaagctga ttcttttggg taacgcttct
    19501 gggtttcaaa atcaacataa aacaagccat accttttgtt atacccattt gtccatgaga
    19561 aaagatccat aagcgaccac aaaaaatacc ccttcacatt taccccggct gttatcgcct
    19621 tgcttaagga ttccaaatag actctcaaat agtcgattct cggctgatcc ataataatgc
    19681 catcttcaaa ctgatcttta taccccatcc cgttctcagt gatatagatt ttgttgtaat
    19741 gtgggtaatc gcttttaatt cgcagcaaca agtcatatag accttctgga taaatcagcc
    19801 agtcccaatc agttcgagga attccttctt tataaatacg ttctccgatt cccttcactt
    19861 tataaacaga ggtccctttt tcacccgtac cgttatgatg gattgcattt tctccatcgt
    19921 aagctttgac aaaatggcat tggtagtgat tgatccctaa gtagtcatta cgcgtggatg
    19981 cttttttcaa ttcaacgaaa tcttcttcag gaaaatgata gctggcctga tttgcttcac
    20041 atatctcatc aagagcagtc aaggtttcag ttgaataata gcctaaatag gtagcatcta
    20101 ataagaaacg gatcgacaat gcatcgtcta aaaaggcagc atgcttatct tccggcgcgt
    20161 ctgttgccgc atatttcgtc tctaatgagt gaactacacc aatttcaccc ggaagttcgt
    20221 tttctttgaa atagttcact acacgggcat gagcaaccat catattatga agacaggcga
    20281 cgatctttgt aaaatcatat ttgattcctg gtgggaaaac acctaacaaa tattgattgg
    20341 ttgcaactgg atagatttca ttaaaggtac tccacacctt gacttctttg aattcatgaa
    20401 aacaaaaaat ggcataggaa acaaatgctt ctattgtttc tcgattcaaa aaatctccat
    20461 ggtcaaacaa acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc gttacgtttc
    20521 ttttcttaca ttcttcgaac acacgatgat aaaaagcgat tccttctgga tttggtttcc
    20581 cagctccatc aggaaaaatg cggctccaag caatggataa tcttagacca tttacaccaa
    20641 aacgttcaca caattcaatg tcctttgcat attgatgata aaaatcactt gccggatcgg
    20701 gactgaatcg cccttgttcc tctaaaaagt catcccaagc aacagcgcct ttgccacctt
    20761 ccttagtggc tccttcaacc tgataggctg ctgtggctcc gccaaaaata aaatcttctg
    20821 gtaatttaag catgtacatc tctcctcaaa tctataatca gttttcgctc tttctaactg
    20881 attgcattgt aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa taatcataaa
    20941 aaccagaaaa cagcaataaa atctattgta acaaacataa aaaccaccct aaatgtttac
    21001 ttttgttcga attagattga ctgttgtccg tttatatgat agattatcct taaattagag
    21061 gtgaagacaa tgttgaaaaa agaaagattg cttactatcg tagaaatggt caataaaaaa
    21121 ggaatcctta ccgttaatga aatcatcaat caattagatg tttctgatat gaccgttcgc
    21181 agagatttag atgaattaga aaaatcgggc aagctgctgc gtgtccatgg aggggctcaa
    21241 agtctttcct acactttaga tcaagaactt tctcacactg aaaaagccac tctgcagatt
    21301 caagaaaaaa aagaaattgc tgccacagca gcctccttga ttcacgaggg agaaacgatt
    21361 ttcttaggac caggtacaac aatcgaacta ttggcgacgc atttactgaa caagaaaatt
    21421 cggatcatta caaataatta tccagttttt gatattcttg ctcaagcaga tggaccagaa
    21481 attctactga ttggcggtga tttccggaaa aataccggcg catttgtcgg accgatcacc
    21541 aacgacaatt taagaagact aaagtttaca aaagcattta tcagttcaaa tggcgttcac
    21601 aacgaagcga tctctactta cagtaccgaa gaaggagaag cacagcagat cgctctaaat
    21661 aattcccgta agaaatatct actcgttgat aataaaaaat tcaatcgaga tgatttttat
    21721 gtgttttata atttgcatga tttcgatctg ctcattacgg atcgccagat ctcgcgggag
    21781 gttcaagagc actatgcaca atatgtcgat ctgcaaattg ctgattcaac cgaaaatacc
    21841 actataggag cgtaactatg aaagggatta tatttgattt caacggaaca atgtttttgg
    21901 actctcatct gcatgaagct gcttggctgc atatgataca tgatcataca aaagatggtt
    21961 tatcagacga ggatatttta aaaaatattc atggtcggac gaatacggag attttgaaac
    22021 atttcatttc agagcatttg accgcctctg agatcgccgt attgtcagaa gaaaaagaaa
    22081 gctattatcg tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca aaagggcttg
    22141 aagcgactct tgaccagctg actgaccaac agattccatt aacgattgcc acggctactg
    22201 tcaaagagaa tgttgctttt tattttgata ttttcgattt agcccgctgg tttgactggg
    22261 acaagattgt ctatgacgac ggctctttcc ctgggaaacc gcaaccggat atttttctta
    22321 aagcggcaga caaactgagc ttagctccgg ctgattgtct tgttatcgaa gatgcttact
    22381 caggattact tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt gatccattcg
    22441 gtaaaaacaa aacagtattt gaacaggcac agctaaataa agatggaacc atcaaagatt
    22501 ttactggttt ttggacagag cacctgtctg ccacatccgt taattcttaa acgattcaac
    22561 taactaccaa agaaaagcaa ctgaaatgca cacctccttt tataaaaaac tgccagtgaa
    22621 acgacatgaa agatacactg ctatatcctt agtgaaatat agcagtgtat ctttgacttt
    22681 ttcattcttt taaccatatg acgcgttcat ctctatctat aattaacatt aaaaatagtg
    22741 ttttcacatt atttctcaaa ccaatcttaa aaatcatcag gagtactttt ttgatatact
    22801 tttattaatg ctgggcgtaa tggttaaaag atttccacta gttcctctcc accaagctga
    22861 gttgaaaaat gcttatctgt aaacaacata gacaaagagg aaataaacgt ttccgtatct
    22921 ttctctgtcc caaggatatt aattaatttt cctactaggt aagtatcatt aaacttaaga
    22981 tctgccttca catcaaaagt tttgataaaa gcttttatca tgcgtacaat tttgtcttta
    23041 tcgccataat tgatttcacc actcatatca atatagtcac taatccctga agaaataatg
    23101 gcaactgctt cttctttcgc cttatcagat aattcattta atgactggga ttgattgatg
    23161 acttcttgag ttttatctgt tacgtaatct ctcattatcc tatcggcata ctccgcaaag
    23221 taatcttgaa aaaattcttc tacagggtaa ccaacttcgt tttcccaacc aggagaaatt
    23281 tcattatcag tacttattaa atctattaaa aaccaacccg ttctctgagt gttgattcta
    23341 taatattttt aatttcctat ataaatcaaa aatattatag aatcaataag ctaattaaga
    23401 aacaaacttt agaaactcta gaagaacttc tgaatttata tagaagtgtg tagctactta
    23461 ctaaatatac aaaagttaaa ataaaatact ctggatactc tttatactgc attaaagtta
    23521 gtaatattaa cagcaaagat atcgaaaaaa caataacata atatgtgtca aaatttttca
    23581 taataagaac tcccttactg ttgcatacta acataatcca aaaactcttc tacttcagct
    23641 aaagacattc cactttcttt agtccaaaaa acagtttttt ctatcgatcc ttctctgtaa
    23701 actgtaaatg tcggagtcga gttaattgag tagcgactct tgaattcttt ccactcattt
    23761 tcatcttctc taacttttgc tacattcacg taataaatat tttcttttcc cttttctacc
    23821 agcatatcga atagcttcct gcttttgatg atcgatttct tgtgctacat ctagtaaatt
    23881 ttcatcaatt tctatatcat aaagtatttc actagtgtct acatccacaa tatatacttt
    23941 ttatcagtag caatttttga gtaatccgtt aaaatgatct cagtctcgtc actttcatca
    24001 gtataaatat acgttaacaa tgaactcacc ttttcaatcg gtgttagctc taagcctact
    24061 gtatttttta ctattgagtc attatttacc accataactt cgtactgtat ataccatcct
    24121 atttggtttc catcaatatc agtaattggc tcactagcta atggagtctc cggaacaata
    24181 ttttcctgat atttcttaaa ctcttctgta gcacgaaaaa ggttatcatg ttcctcactt
    24241 gaaactggtt gagtcataga aaatgttact tggctaaaca gagggaaaat aaacactaca
    24301 atcaacaaag tataaaaaaa agcccttctt tttcattttt tatcttccaa tcacaaattg
    24361 aaaaacttat tcctgcaact ttgagtctag tatcacaatc gactgactac aactattcat
    24421 ttacattcta ctttaagttc ctccttaacc attaatttta ttaaagatac ttgtaatagt
    24481 gattctcaca aaatttcatg aaaaatactt tattactgta tctaaaattc caatacacaa
    24541 cttatcgact attatctgaa aaccaaaact tattattcct tgatgttatt ctagcaatag
    24601 taactgcttt tttatttttt tgcatatttt ttcggcgtta ctttttaaag ttaacttttt
    24661 cgacaaatag aaagagatac gaatcactga tttttttgaa gaaaagaaat ggagtgagct
    24721 gaacgttaga gatattggga gctttcgtaa ctgcttatta aacgataatg gttatttgca
    24781 accgtattgc tcgcttctac atgaaatatt tcatgaaata tttcgcaaaa cctttgatta
    24841 tgtagttacg tttccattct tgactaaaaa tgtttcaaaa acgaacaaag gtgttaaaag
    24901 gtaaagtgat caatactact ggtcaaaata agaggttgaa agggaattga aaaataacta
    24961 cagataatac ttttataaac gtacgtgctt tgtggttctg tagttaacta tataactgga
    25021 attagggaat ggtatcaatt tgaaaagaaa gattctacca atttatcata tgttaaaaat
    25081 ggaaaatcaa cagcagttct ttcggaaacc ttacac
//
LOCUS       DAYYNQ010000037        24446 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000037 DAYYNQ010000000
VERSION     DAYYNQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.037"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    171..376
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            488..1744
                     /gene="tyrS"
                     /locus_tag="ACHBJ1_001366"
     CDS             488..1744
                     /gene="tyrS"
                     /locus_tag="ACHBJ1_001366"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM9430315.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            2035..3912
                     /gene="tdc"
                     /locus_tag="ACHBJ1_001367"
     CDS             2035..3912
                     /gene="tdc"
                     /locus_tag="ACHBJ1_001367"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9430316.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            4155..5576
                     /gene="tyrP"
                     /locus_tag="ACHBJ1_001368"
     CDS             4155..5576
                     /gene="tyrP"
                     /locus_tag="ACHBJ1_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM9430317.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            5685..7052
                     /gene="nhaC"
                     /locus_tag="ACHBJ1_001369"
     CDS             5685..7052
                     /gene="nhaC"
                     /locus_tag="ACHBJ1_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM9430318.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(7333..8352)
                     /locus_tag="ACHBJ1_001370"
     CDS             complement(7333..8352)
                     /locus_tag="ACHBJ1_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430319.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8374..9381)
                     /locus_tag="ACHBJ1_001371"
     CDS             complement(8374..9381)
                     /locus_tag="ACHBJ1_001371"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9430320.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(9374..10039)
                     /gene="pgmB"
                     /locus_tag="ACHBJ1_001372"
     CDS             complement(9374..10039)
                     /gene="pgmB"
                     /locus_tag="ACHBJ1_001372"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM9430321.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(10032..12326)
                     /locus_tag="ACHBJ1_001373"
     CDS             complement(10032..12326)
                     /locus_tag="ACHBJ1_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM9430322.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            12586..14751
                     /locus_tag="ACHBJ1_001374"
     CDS             12586..14751
                     /locus_tag="ACHBJ1_001374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM9430323.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            15055..15888
                     /locus_tag="ACHBJ1_001375"
     CDS             15055..15888
                     /locus_tag="ACHBJ1_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM9430324.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(15990..17690)
                     /locus_tag="ACHBJ1_001376"
     CDS             complement(15990..17690)
                     /locus_tag="ACHBJ1_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430325.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            18197..19357
                     /locus_tag="ACHBJ1_001377"
     CDS             18197..19357
                     /locus_tag="ACHBJ1_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM9430326.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            19373..19816
                     /locus_tag="ACHBJ1_001378"
     CDS             19373..19816
                     /locus_tag="ACHBJ1_001378"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430327.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(20299..20829)
                     /locus_tag="ACHBJ1_001379"
     CDS             complement(20299..20829)
                     /locus_tag="ACHBJ1_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM9430328.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            20934..21485
                     /locus_tag="ACHBJ1_001380"
     CDS             20934..21485
                     /locus_tag="ACHBJ1_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9430329.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(21638..22111)
                     /locus_tag="ACHBJ1_001381"
     CDS             complement(21638..22111)
                     /locus_tag="ACHBJ1_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9430330.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            22249..23721
                     /locus_tag="ACHBJ1_001382"
     CDS             22249..23721
                     /locus_tag="ACHBJ1_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9430331.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            23718..24428
                     /locus_tag="ACHBJ1_001383"
     CDS             23718..24428
                     /locus_tag="ACHBJ1_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM9430332.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         7616 a         4395 c         5063 g         7372 t
ORIGIN      
        1 taaattgcag aaaaaataca gaaaaagtta tttcttttaa caataattga aaataaaatg
       61 ttcggaatta gagcggattc aaaaaagaaa aaatgaggaa tttaaaaaaa atatcctttt
      121 tctgttgaca aagaaaaagt tgcgctctat gatagacaca tattaataat aacaagacga
      181 agatcgaaac gaagtagttg ttaaagccaa tgtttcagaa agctggtggt cgctgcaaac
      241 cagtacatgc tttaaagcga attacatttc aggagtcttt ggcagtgatt gccacgcgac
      301 agcgttaaat gttcgagagg tatgttggat atctagacat acaaattggg tggtaccgcg
      361 agatttcgtc ccttggctta tatgccaagg gacttttttt atttcctatc ctatcctgaa
      421 gaaataatta attacatatg ctagcaggtt cctgctttca taaaaaaact acacggaggt
      481 cagtaaaatg aatattattg atgaattaac ttggcgcgac gccattaatc agcaaacaaa
      541 cgaagaaaga cttcgcgagc tcgttgaaga aaagagcatt tcactttatt gcggcgttga
      601 tccaactggt gattcgatgc atatcggaca tttgattcca tttatgatga tgaaaagatt
      661 ccaattggca ggccatcatc catatattct gattggcggc ggaacaggaa cgatcggcga
      721 cccaagcggc cgcaaaacag aacgtgtgtt gcaaacgatg gaacaagtgc aacataacgt
      781 ggacgcactt tccaatcaaa tgagaaaatt gtttggtaaa gatgcaaata tcacatttgt
      841 gaacaactac gactggttgt caaaaatctc tttacttgaa tttttgagag actacggtaa
      901 aaacttcaac atcaacacga tgttagcaaa agatatcgtt gccagccgtt tggaagtagg
      961 aatctcattt acagagttta cttaccaaat cctgcaatct atcgactttt tgcatctgca
     1021 taaaacatac gatgtgcaat tgcaaatcgg tggagcagac caatggggaa atatcacagc
     1081 tggattagac ctaatccgaa aattagaagg accagaagca gaagcgttcg gtttaacgat
     1141 cccattgatg ctgaaggctg acggaacgaa attcggaaaa actgcaggcg gtgccgtttg
     1201 gcttgatccg aagaaaactt caccatttga gttctaccaa ttctggttga atcaagatga
     1261 tcgcgatgta gtgaaatact tgaaattctt tactttcttg tctcaagaag aaatcgaaga
     1321 cttagcgaaa aaagtagaga cagaacctga gaaacgtgaa gcacaacgcc gtttggcaga
     1381 agaagttaca agatttgtgc atagtgaaga agacttgaaa gaagcgcaaa aaataacgca
     1441 agcattgttc tctggaaata ttaaagaact aaatgcggaa gaaatcgcgc aaggattcgg
     1501 taaaatgccg aacgttgaaa tttcaagcac acctgaaaat atcgtggaac tgcttgtttc
     1561 cacaaaaatt gaaccatcta aacgtcaagc acgtgaagat gtttcaaacg gagctataag
     1621 tattaacggt gaccgcgtta ccgatttaaa ttttgtcata aatccatccg atgaattcga
     1681 tggtaagttt gtggttattc gaaaaggtaa gaaaaattac tttttggcca aagtaattga
     1741 ttagaattta gtaagttttt accttgaaaa agggaaatac tattacaaca ttccaatttt
     1801 gaaacccatc ctaaaataga acgctggttg tcgttaatac aatctttgcg agggagttta
     1861 catttgtaaa ttccctcgtt ttttttaatt atttcaaaag tgtgaacaat tcggataatt
     1921 gatacatatt aaaatatgat taaaaatcag gccaaaactt agtttacaga atagaagaac
     1981 aggtgtaatg tagggctcga caaaatttaa atattttttt aggagggttt cactatgagt
     2041 gaatcattgt cgaaagatct aaacctaaat gccctattca ttggggacaa agctgagaac
     2101 ggccaaatct ataaagcttt gttgaatgaa ttggtggatg aacatttagg ctggcgtcaa
     2161 aactacatgc ctcaagacat gcctattatt acgccagaag aaaaaagcag tgctagtttt
     2221 gaacacactg tgaataaaac aaaggatgtt ctatcagaaa tttcagcgcg gatgcgtacc
     2281 cattccgttc cttggcataa tgccggacgt tactggggtc atatgaactc tgaaacattg
     2341 atgccatctt tactagcgta caattttgct atgctatgga atggtaataa cgttgcctat
     2401 gaatcttctc ctgcaacaag ccaaatggaa gaagaagtag gaatggaatt tgcaaaatta
     2461 atgagctata aagatggctg gggccacatc gttgctgatg gttctttagc aaacttagaa
     2521 ggactttggt atgcccgcaa catcaaatca ttgccgcttg caatgaaaga agtgacacct
     2581 gaactagttg ctggaaaaag cgattgggaa ttaatgaact tgtcaacaga agaaatcatg
     2641 aatctgttag acagcgtccc agaaaaaatc gacgaaatca aagctcactc agcacgtagc
     2701 ggaaaacatt tagaaaaatt aggaaaatgg ttggttccac aaactaaaca ctattcatgg
     2761 ctaaaagctg ccgacatcat cggtatcggt ttagaccaag tgattccagt gccagttgac
     2821 cacaactatc gcatggacat caacgaactt gaaaaaatcg ttcgtggtct agcagctgaa
     2881 aaaacaccaa tcctaggtgt agtcggtgtt gtaggatcaa cagaagaagg agcaatcgac
     2941 ggtatcgata aaatcgttgc attgcgtcgc gtattagaaa aagacggtat ttacttctac
     3001 ttacacgtag atgctgctta tggcggatac ggccgtgcga ttttcttaga cgaagacaac
     3061 aacttcattc catttgaaga tttaaaagat gttcattaca aatacaatgt ctttacagaa
     3121 aacaaagatt atatcttaga agaagtacac agtgcatata aagcaatcga agaagcagaa
     3181 tctgtaacga ttgacccaca taaaatgggc tatgttccat attctgccgg tggtatcgtc
     3241 atcaaagata tccgcatgag agacgttatc tcttactttg cgacatatgt atttgaaaaa
     3301 ggcgcagata tcccagcatt acttggggct tatatcttgg aaggttcaaa agcaggggca
     3361 acagcagcca gcgtttgggc agcacaccat gtcttacctt tgaatgtcac aggatatggt
     3421 aaattaatgg gtgcatcaat cgaaggtgca catcgtttct acaacttctt gaatgacttg
     3481 tcatttaaag taggagacaa agaaatcgaa gttcatccat taacttatcc agatttcaat
     3541 atggtagact atgtattcaa agaaaaaggc aacgatgact tagttgcaat gaataaattg
     3601 aaccatgacg tatatgatta ttcttcatac gtgaaaggaa gcatttacgg taatgagttc
     3661 ttaacttcac atactgactt tgctatccca gactatggca acagcccatt acaatttgtg
     3721 aaccaactag gattctcaga tgaagaatgg aaccgtgccg gaaaagtaac tgtattgcgt
     3781 gccagcgtaa tgacaccata catgaacaaa gaagaacact ttgaagaata tgctgaaaaa
     3841 atcaaagcag ctcttcaaga aaaattggaa aaaatctacg cagaccaatt attggcaagc
     3901 gaagcaaaat aagcaaggta atcatgttag aaaacagctt gaatactaac tgtttctgac
     3961 gcatgtttgg atctgaagag caaggggaga cctaagctct tcgatccctt tgttttgagg
     4021 ttgtctttga agaaaccctt caagcaataa actcaaagaa tcccctcaaa tattcgctga
     4081 attgtttcgt ttattgctga aactacttca aagacaccgt tattttatcc aaaaaatcaa
     4141 ggagagtgtc aacggtgagt aaatctgaag taagtaaaat gggtcttggg acctttatcg
     4201 gtctgacaat ggcactgtgt gcaacggttc ggagtattcc tacgttagcc gcagtcggtt
     4261 ggacattgat tttttattca atttttgcag ttttgttttt tgccggtccg atatcaatgg
     4321 tttccggtga gctttccacg atgcttcctc aagaaggcgg tcctcagtta tgggtaaaaa
     4381 cagcactggg cagcaaatgg ggatttgtcg ttgcatggct attatgggtg caaatgttcc
     4441 caggtatggt aatggtcgct tctactttag gaccattgtt aggaaacaca tttggtaatg
     4501 tagaacttgg caataatcac tggttcgttt taggttgtat tttagttatc tactggatca
     4561 tcacgatctt gaacttgaaa ttcgatatgg caaaagtcgg cgggaacatc ggtgtatggc
     4621 taggggtata tatcccagta gtcatcatgt tcgttttagg tgttttggca gccttcaaag
     4681 tcggactagt ctcaaacggc tatctaggtg acttttcatg gtcaaaagct ttccctgact
     4741 tggaacatat tgattcattg aaatacttag ctggtatcac atttattttt gttgggatcg
     4801 aaatgagttc tgtttacatg cctcgtttga aagacgcaac aaaaaattat acaaaaggcg
     4861 tatttatcgc tttgatcgga ttagttttat tgaatgtcat caatgcgatg cttgtagcaa
     4921 atgtcgtacc agatggaaaa atggaattag caaatatcac gcagcctatc ttgattgact
     4981 gtcaaatcct aggcttacca gaagtgatcg gaaatatctt cagcttcatg gtatttattg
     5041 gtgtgctttt acaattatca gcttgggtta ctggaccatc aaaaacaatc atccaagtag
     5101 ctcgtgaagg attccttcca ccaaaattcg gattccataa agaaaacaaa tacggcgtat
     5161 ctagaaatgt cgttctaaca caatcaatcg ttatttcatt atttgctttg ctttatggtg
     5221 taatggatga cgtaagtgct gtattcttga cacttacaaa tgctacaact gttatttatt
     5281 gtatcgtgta cattttgatt gctgtttcat tgcttaaaat gcgtaagaaa catccagaat
     5341 tcgaacgtcc ttaccgaatt ggtaaaaacg gaaacggatt agcttgggta gtcagctgca
     5401 tgctgatctt ctctatcatc gttgtcgtat ttgctacatt aggtacagcg acattgagtg
     5461 atgcattatt agtagcagct atcacagtgg tcatgttcgt tattccattg atcatcaatc
     5521 acttcaagaa agattcttgg gggattgagg tagaaaaaag tttagaagaa aaataaaaaa
     5581 ataagtcttt cagccgacgt tttgtcgctg atttagtagg aggtcctgcc aagtgaatag
     5641 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
     5701 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
     5761 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
     5821 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
     5881 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
     5941 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
     6001 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
     6061 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
     6121 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
     6181 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
     6241 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
     6301 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
     6361 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
     6421 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
     6481 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
     6541 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
     6601 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     6661 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     6721 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     6781 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     6841 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     6901 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     6961 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     7021 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     7081 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa tttttgtgaa
     7141 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     7201 gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa caatgtgtta
     7261 gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca gcgtatttag
     7321 tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt tgatcaagcg
     7381 atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt cgcgcagctt
     7441 ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa ggtaagggtg
     7501 tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct ctggaatgac
     7561 atagccatac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt caatgaccac
     7621 caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat cctccggatg
     7681 ctctagtact acactggata acgcaggaag accagccatc atcattgctt tttgataacc
     7741 gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca ggatattctt
     7801 atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt tatcattatc
     7861 tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga aagggatgtg
     7921 gttttggtat aaataatcaa tgacagtatc ttgactatct gaatacacta gaataaaacc
     7981 atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt caaaattttt
     8041 agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga ttgcttccat
     8101 gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagagggg gtaagattac
     8161 gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag gaacataacc
     8221 tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac taggatgatc
     8281 gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca catcttttac
     8341 ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc gataaatcgt
     8401 ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg ggaaatgaac
     8461 gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca tctgttttcc
     8521 attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac ttttattaga
     8581 agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg aagttttgga
     8641 aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac atgcaaggat
     8701 ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt ttgtccaatt
     8761 tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga ccgttaactg
     8821 atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat ggttggtcgg
     8881 attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt cgttttttgt
     8941 taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt catcttttaa
     9001 gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc aaaattggaa
     9061 agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct ccaatctatg
     9121 ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg ctccaaaaaa
     9181 gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat tttctgattt
     9241 tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga gaagaacgac
     9301 ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa ctggtacgac
     9361 ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg atcgtaagaa
     9421 aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt cccacgccaa
     9481 ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct tcgatcccga
     9541 tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata tctggcgcag
     9601 gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt gtaagaccca
     9661 ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc ttgatttttt
     9721 cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt ggttcgatcg
     9781 tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca aattcttcct
     9841 ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag tcttctcggc
     9901 tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt tcctgaccaa
     9961 gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg tctaaatcaa
    10021 acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag acgatccgct
    10081 aaagaataag actgttcata gacgtaaatt tctagaggag aaccttctaa taactcgaaa
    10141 gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt gatatggaaa
    10201 gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc atgggcagtt
    10261 ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga agtgatgtgc
    10321 aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt tcgctggtac
    10381 atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg gatacttggc
    10441 gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt ttgcttttct
    10501 tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc ctgcttgatg
    10561 aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag atcgcttgtg
    10621 ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg gacaaagatt
    10681 cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca tttggctagt
    10741 tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt ttctcgcgtg
    10801 taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat attgttctca
    10861 tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt tgaaaaatgg
    10921 acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc atttttcaaa
    10981 tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat cgcgccattt
    11041 cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc agtgaaggtg
    11101 accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat tgcttgcggc
    11161 agttgattat gccgatattt cagcagattc ttagttactt caggtttggc taaagcaaga
    11221 taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta tttttctcct
    11281 gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt agagaatagt
    11341 tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat gaccacatcg
    11401 gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg gatataattg
    11461 tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc tggaatatct
    11521 ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg agataattct
    11581 gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat gactgatttt
    11641 ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc aaagttgttt
    11701 ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc aatctcattc
    11761 caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa ttttgatacg
    11821 agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata agctgattct
    11881 tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt agaagtagtg
    11941 attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta aaagtcttct
    12001 ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat cgcgataaag
    12061 ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca ccagccgaca
    12121 cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg gtcccctgag
    12181 taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact tgttaaagat
    12241 tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca tggatcaata
    12301 gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt tatacgttca
    12361 taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca tagaaaaaag
    12421 cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacgtat tataggtttt
    12481 atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga aaacgcacgc
    12541 tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa aaagatattt
    12601 agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc agttatgccg
    12661 gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc tgattggacg
    12721 ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat tggtaatctt
    12781 catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc aggcggagca
    12841 tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat ctttggtgtc
    12901 acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg tacgaagatc
    12961 atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat gggagtattc
    13021 gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta taattataga
    13081 aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt tgttgttatc
    13141 ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt acaggctgga
    13201 atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat tctagcaccg
    13261 tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca tatgctgacg
    13321 attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg cgcacaggcg
    13381 gggacacaag tatttgggca agatccgtta tggttggctt gggccactga tttagtgaat
    13441 cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg gacgccggct
    13501 cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat ggcttttgcg
    13561 atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta tttttcagca
    13621 gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt tatgtttgct
    13681 gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc gatggcagat
    13741 atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg gacaccgtta
    13801 gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt gatcgctttt
    13861 ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt tgctacaccg
    13921 ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa cagcggaaca
    13981 ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa agaaaatatc
    14041 aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg agaaaaagta
    14101 gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa agacaatgga
    14161 gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga agatctgctg
    14221 caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg acaagcaggt
    14281 agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg agaagtgatt
    14341 cctttatctg aagtaaaaga tccggtattc tcacaaaaaa tgatgggtga cggctttgct
    14401 gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac aagtattttc
    14461 cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt gatccatatg
    14521 ggaatcgata cagtccaaat gaatcagcca gcttttgaga ttgctgtcaa agaaggccaa
    14581 gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca aaacgaagaa
    14641 aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt agtgatcaag
    14701 caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta aggagaggtt
    14761 gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag ctcctcctat
    14821 ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt tagtggagag
    14881 gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa atagctcctg
    14941 gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg agaggttgtg
    15001 acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa accaatgaaa
    15061 ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa attgcaagaa
    15121 ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga aatcaaccaa
    15181 ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtcc ggtaaagaat
    15241 caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt aaaggaacac
    15301 ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa atatgaagaa
    15361 ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg tccttaccgt ctcccaattt
    15421 caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa gatagatgat
    15481 caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa aagtggcttt
    15541 tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc actttttttc
    15601 ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag agaaagttgc
    15661 cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc tactgtggaa
    15721 aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt tattttcgtc
    15781 cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga tagtccaatc
    15841 atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa aatataacat
    15901 ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt ttggagagtt
    15961 ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta tctatgataa
    16021 ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat gaaatagaaa
    16081 tagattggcg tttgataatg aagcgtgagc tcgttgattc ctttttgctg cttcacgatt
    16141 ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg ctgtagcaca
    16201 gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt tttccacttg
    16261 attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc tagtatttct
    16321 tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag atagtcaggt
    16381 gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa tgttttcctt
    16441 gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc attttcccac
    16501 ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag aatgccaaat
    16561 atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag ggcaaggtaa
    16621 agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat caaactaata
    16681 aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga aagaaaaaag
    16741 aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg ttgaaagaaa
    16801 ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta atggagccaa
    16861 taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa gggaggaaga
    16921 atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt cagaacaaaa
    16981 taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt ctggttaaaa
    17041 aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga cagtaaatgg
    17101 atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt tttatgaagg
    17161 acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca gctagtgaat
    17221 ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac ggctactggt
    17281 acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga aaatcctgaa
    17341 ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc tgcaagcaac
    17401 acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac gatcctgcca
    17461 ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt aatttgttgc
    17521 gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat tagtttatgt
    17581 gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa aaatgggcca
    17641 tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat atatcctatg
    17701 cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt acaagaaaaa
    17761 cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat ctccttgttt
    17821 tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc tctcggtaaa
    17881 cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat atgagaagct
    17941 attttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa cctcttcctt
    18001 aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa aaatatgagt
    18061 agctattttt ttgggctgta cttattactt gatgcaggac agctttttca caacctcatc
    18121 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
    18181 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
    18241 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
    18301 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
    18361 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
    18421 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
    18481 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
    18541 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
    18601 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
    18661 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
    18721 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
    18781 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
    18841 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
    18901 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
    18961 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
    19021 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
    19081 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
    19141 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
    19201 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
    19261 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
    19321 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
    19381 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
    19441 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
    19501 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
    19561 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
    19621 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
    19681 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
    19741 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
    19801 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
    19861 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
    19921 ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
    19981 tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg atttcacaac
    20041 ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta gcctagaaaa
    20101 tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc tgatttcaca
    20161 acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    20221 acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg ctgatttcac
    20281 aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt attttccgct
    20341 acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt cattgtctat
    20401 acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt ttggataagt
    20461 ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga aagtaacagt
    20521 gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa gtttcttttc
    20581 atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag tgattttgaa
    20641 tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac ggcctttgac
    20701 cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc attcatattc
    20761 ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca attctttaaa
    20821 ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta catgttttcg
    20881 attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa
    20941 gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag atgacaggat
    21001 atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca aaacatagtc
    21061 agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat caagtagaga
    21121 tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg aaggaacaat
    21181 tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac gaatttgttc
    21241 tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt attcagcagc
    21301 agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa acgatctttc
    21361 ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg catgcgatca
    21421 atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt aaccgaaaaa
    21481 aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga aacgcagatc
    21541 accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg tagcatctac
    21601 ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt aacaactgtt
    21661 cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct gtctcgttgt
    21721 catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt aaaatcgtat
    21781 agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta cggacatggt
    21841 gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta aatccgtcat
    21901 ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat tttctaggac
    21961 aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac cttaattgac
    22021 gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat tgatcatttt
    22081 ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc ctcttgcttc
    22141 ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc gaatttagga
    22201 ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac
    22261 caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt tgtcttatta
    22321 attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt tcctacattg
    22381 atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg tttcttgcta
    22441 gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag ttcaagaaat
    22501 ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt tacagcacct
    22561 aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg agtatcgatg
    22621 ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg atcagcaatg
    22681 gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt atctggttat
    22741 atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat cgttgttctt
    22801 gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa tcctagcatt
    22861 gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta cggattttct
    22921 agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt tgttggtgtg
    22981 attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt cttggaactt
    23041 cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt aacaaatatg
    23101 gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg tggagaatca
    23161 gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc tatgatgcct
    23221 gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac agggatgttc
    23281 attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc agttttatat
    23341 atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat gccggtaacg
    23401 acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc ggtaaataat
    23461 acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt gttgacgaat
    23521 gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt agcttataaa
    23581 gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt agcgatcatc
    23641 tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc tgttctaatg
    23701 aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt gcttttgctt
    23761 taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt ttcggactaa
    23821 tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa
    23881 aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa ggagcagata
    23941 tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac cgaacaaatg
    24001 aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa gtagaaaaga
    24061 cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt
    24121 ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga gtgaatacat
    24181 tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag ttagctgaat
    24241 tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt caagacggta
    24301 tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa caacgtacga
    24361 aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa gaagcaaaag
    24421 gcgaataata ttttgaacac catgta
//
LOCUS       DAYYNQ010000038        24426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000038 DAYYNQ010000000
VERSION     DAYYNQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.038"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHBJ1_001384"
     CDS             complement(53..787)
                     /locus_tag="ACHBJ1_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM9430333.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHBJ1_001385"
     CDS             1114..1524
                     /locus_tag="ACHBJ1_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM9430334.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHBJ1_001386"
     CDS             1517..2218
                     /locus_tag="ACHBJ1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM9430335.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHBJ1_001387"
     CDS             2218..3804
                     /locus_tag="ACHBJ1_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430336.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHBJ1_001388"
     CDS             3823..4044
                     /locus_tag="ACHBJ1_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM9430337.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHBJ1_001389"
     CDS             4041..4802
                     /locus_tag="ACHBJ1_001389"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM9430338.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHBJ1_001390"
     CDS             5182..5691
                     /locus_tag="ACHBJ1_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM9430339.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHBJ1_001391"
     CDS             complement(5766..6305)
                     /locus_tag="ACHBJ1_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM9430340.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBJ1_001392"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHBJ1_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM9430341.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHBJ1_001393"
     CDS             7318..8169
                     /locus_tag="ACHBJ1_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM9430342.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHBJ1_001394"
     CDS             8197..8832
                     /locus_tag="ACHBJ1_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM9430343.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHBJ1_001395"
     CDS             8852..9625
                     /locus_tag="ACHBJ1_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9430344.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHBJ1_001396"
     CDS             10033..10830
                     /locus_tag="ACHBJ1_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM9430345.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHBJ1_001397"
     CDS             10808..11221
                     /locus_tag="ACHBJ1_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9430346.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBJ1_001398"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHBJ1_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM9430347.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBJ1_001399"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHBJ1_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM9430348.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHBJ1_001400"
     CDS             15998..16558
                     /locus_tag="ACHBJ1_001400"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM9430349.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBJ1_001401"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHBJ1_001401"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM9430350.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHBJ1_001402"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHBJ1_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM9430351.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHBJ1_001403"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHBJ1_001403"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM9430352.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHBJ1_001404"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHBJ1_001404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM9430353.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHBJ1_001405"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHBJ1_001405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM9430354.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHBJ1_001406"
     CDS             21905..22483
                     /locus_tag="ACHBJ1_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430355.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHBJ1_001407"
     CDS             22535..23017
                     /locus_tag="ACHBJ1_001407"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM9430356.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHBJ1_001408"
     CDS             23134..23508
                     /locus_tag="ACHBJ1_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430357.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..>24426
                     /locus_tag="ACHBJ1_001409"
     CDS             23938..>24426
                     /locus_tag="ACHBJ1_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM9430358.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4390 c         5213 g         6816 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggac
//
LOCUS       DAYYNQ010000039        24414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000039 DAYYNQ010000000
VERSION     DAYYNQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.039"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..884)
                     /locus_tag="ACHBJ1_001410"
     CDS             complement(36..884)
                     /locus_tag="ACHBJ1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9430359.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="ACHBJ1_001411"
     CDS             complement(1108..3048)
                     /gene="thrS"
                     /locus_tag="ACHBJ1_001411"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM9430360.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3385..3564)
                     /locus_tag="ACHBJ1_001412"
     CDS             complement(3385..3564)
                     /locus_tag="ACHBJ1_001412"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM9430361.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3611..5434)
                     /locus_tag="ACHBJ1_001413"
     CDS             complement(3611..5434)
                     /locus_tag="ACHBJ1_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9430362.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5712..7991
                     /gene="helD"
                     /locus_tag="ACHBJ1_001414"
     CDS             5712..7991
                     /gene="helD"
                     /locus_tag="ACHBJ1_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM9430363.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8084..8731)
                     /locus_tag="ACHBJ1_001415"
     CDS             complement(8084..8731)
                     /locus_tag="ACHBJ1_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430364.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8910..9629)
                     /locus_tag="ACHBJ1_001416"
     CDS             complement(8910..9629)
                     /locus_tag="ACHBJ1_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430365.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9760..10911)
                     /locus_tag="ACHBJ1_001417"
     CDS             complement(9760..10911)
                     /locus_tag="ACHBJ1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM9430366.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11128..11868)
                     /locus_tag="ACHBJ1_001418"
     CDS             complement(11128..11868)
                     /locus_tag="ACHBJ1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430367.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11872..12195)
                     /gene="rsfS"
                     /locus_tag="ACHBJ1_001419"
     CDS             complement(11872..12195)
                     /gene="rsfS"
                     /locus_tag="ACHBJ1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM9430368.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12252..12845)
                     /gene="yqeK"
                     /locus_tag="ACHBJ1_001420"
     CDS             complement(12252..12845)
                     /gene="yqeK"
                     /locus_tag="ACHBJ1_001420"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM9430369.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12829..13479)
                     /locus_tag="ACHBJ1_001421"
     CDS             complement(12829..13479)
                     /locus_tag="ACHBJ1_001421"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM9430370.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13489..13800)
                     /gene="yhbY"
                     /locus_tag="ACHBJ1_001422"
     CDS             complement(13489..13800)
                     /gene="yhbY"
                     /locus_tag="ACHBJ1_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM9430371.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13816..14925)
                     /gene="yqeH"
                     /locus_tag="ACHBJ1_001423"
     CDS             complement(13816..14925)
                     /gene="yqeH"
                     /locus_tag="ACHBJ1_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM9430372.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14922..15449)
                     /locus_tag="ACHBJ1_001424"
     CDS             complement(14922..15449)
                     /locus_tag="ACHBJ1_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM9430373.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15629..16414)
                     /locus_tag="ACHBJ1_001425"
     CDS             complement(15629..16414)
                     /locus_tag="ACHBJ1_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM9430374.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16407..17276)
                     /gene="accD"
                     /locus_tag="ACHBJ1_001426"
     CDS             complement(16407..17276)
                     /gene="accD"
                     /locus_tag="ACHBJ1_001426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM9430375.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17277..18656)
                     /locus_tag="ACHBJ1_001427"
     CDS             complement(17277..18656)
                     /locus_tag="ACHBJ1_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM9430376.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18664..19083)
                     /gene="fabZ"
                     /locus_tag="ACHBJ1_001428"
     CDS             complement(18664..19083)
                     /gene="fabZ"
                     /locus_tag="ACHBJ1_001428"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9430377.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19108..19584)
                     /gene="accB"
                     /locus_tag="ACHBJ1_001429"
     CDS             complement(19108..19584)
                     /gene="accB"
                     /locus_tag="ACHBJ1_001429"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM9430378.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19587..20822)
                     /gene="fabF"
                     /locus_tag="ACHBJ1_001430"
     CDS             complement(19587..20822)
                     /gene="fabF"
                     /locus_tag="ACHBJ1_001430"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM9430379.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20841..21578)
                     /gene="fabG"
                     /locus_tag="ACHBJ1_001431"
     CDS             complement(20841..21578)
                     /gene="fabG"
                     /locus_tag="ACHBJ1_001431"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM9430380.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21590..22507)
                     /gene="fabD"
                     /locus_tag="ACHBJ1_001432"
     CDS             complement(21590..22507)
                     /gene="fabD"
                     /locus_tag="ACHBJ1_001432"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9430381.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22614..22838)
                     /locus_tag="ACHBJ1_001433"
     CDS             complement(22614..22838)
                     /locus_tag="ACHBJ1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9430382.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22891..23853)
                     /locus_tag="ACHBJ1_001434"
     CDS             complement(22891..23853)
                     /locus_tag="ACHBJ1_001434"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM9430383.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23863..24303)
                     /locus_tag="ACHBJ1_001435"
     CDS             complement(23863..24303)
                     /locus_tag="ACHBJ1_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9430384.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6684 a         5434 c         4249 g         8047 t
ORIGIN      
        1 attctaaaaa acttatataa aaaatagtaa ggtttttatt tcgaatccat aaaatattct
       61 tggattgctt ctcgtgtttt tttcactttt tcgctaagct tttcgttttt agccatatag
      121 aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa gtgaattagt tgccgcactt
      181 cgattcgtga tttcttttgg tctagatgtt tgtacgagta catcggctat cttgctaagc
      241 gggttatttc caaattgcgt caacgcaatg atttcgatat tcatgctttt cgccagctcg
      301 gctaatgcga ctacatctgc acttctacct gagtttgaaa caagccaaag cactttcttt
      361 ttctcattgc tgacaagttg cgggagcaag acgtggatat ctttttcaaa aatgattggc
      421 ttaccgacac gagaccattt ttgcagaata tcttctacta ctaaagaaga ggcgccaacc
      481 ccatataaaa aaacagtctc tgccgattcc aaacactcta cgactttttg gatcgtttct
      541 tcttctagaa tatctactgt ttcttttatc gctacttgtg cattgttacc aagtttgttt
      601 ttgatagagt aaaaggactc atttgcttct atatcactaa aaccttcatc gattccagtc
      661 tgtagttctg ttgaaatcgt gattttgaag tccgtcagac tttgaaaacc gatttttttg
      721 gaaaaacgta caactgtcgg cgcactcaac ccggaagctt ctgctaattc attagctgtc
      781 attgtaggga ccttctcaat attaccgata atgtagttgg ccaacttttt ttctgctgaa
      841 gaataatctt ccatcatact ttcaatacga ctaattacgc tcataagttc ctccaccctt
      901 ttaatgatag tctatgtaag tataccatat cagaccctta ttcacggtcg tatactggat
      961 aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc ccatcaatcc cacagattct
     1021 attgagcatc tgtgtctttg aggaatacct gttatctatt gttcggattg ctttctatct
     1081 actacttttt tacaggaaat ttacctatta gttttctctg ctgtaatttt tgacttctgc
     1141 ttccatagca tcaataaaga tattcaaatc ttctacagct gtttctttac ttccataacg
     1201 acgtacgttc actgttgcat cttccaattc cttgtctcca acaactaatt ggtaagggat
     1261 cttttgtgtt tgagaggcac gtatcttata gcccattttt tcattacgat catcaacttc
     1321 tacacgcaac cccttcattt gcaaacgttc tttgatttca taagcataat cgccatgtgc
     1381 atcaacagaa acagggatga ttgttgcttg tataggtgcc aaccatgttg gaaaagcgcc
     1441 tttgtatact tctgttaaat aagcgacaaa acgttccatt gtagaaacaa ttccgcggtg
     1501 gataactact ggacgatgcg tattttcccc gtcttctcca acatatgtca aatcaaagcg
     1561 ctctggcaat aagaagtcta attgtatagt tgataatgtt tcttctgtac caagagctgt
     1621 tttgacttga acatctagtt ttggtccgta aaaggctgcc tcgccttctg cttcaaagta
     1681 atctagttct aattcatcca tagcggcttt cagcattgct tgggcttttt cccacattgc
     1741 gtcatcatca aaatatttat ctgtattgtt tggatcgcga tagctcaagc ggaaacgata
     1801 atctgtaata ttgaagtcag catatactgc caccatcaat tcaagcgtac gtttaaattc
     1861 atctttgatt tgatcaggac gaacaaatgt atgaccatca ttcaacgtca tttctcgtac
     1921 acgttgtaaa cctgacaaag cacctgattt ttcatagcga tgcatcattc ctaattcagc
     1981 gatacgaatt ggtaattcac gataagaatg gatcgtgttt ttgtagacca tcatgtgatg
     2041 agggcagttc attgggcgaa gaacaagcat ttcgccatct cccatatcca ttggcgggaa
     2101 catgtcttct tgataatgat cccagtgtcc agatgttttg tacaactcaa catctcccat
     2161 tattggtgta tatacatgtt ggtaacctag actaacttct ttgtctacga tataacgttc
     2221 gatcgtgcgt cggattgttg ccccttttgg aagccagaat ggcaaaccag aacctacttc
     2281 tggggaaacc atgaacaagt caagttcttt tcctagttta cgatggtctc tttcttttgc
     2341 ttcttcacgg agacggatat attctttcaa agctttcttg tcaaaaaagg ctgtcccgta
     2401 tacacgttgc atcatttgat tgtttgagtt tccacgccaa taagcacctg ccactgataa
     2461 taatttaaat acttgtatac ggccagtgga tggaacatgt ggtccacgac acaaatcgat
     2521 gaattcgcct tgttgataag cagtaatcac ttcttcttct ggcaattcac taatcagttc
     2581 tactttgtac ggatcacttg caaaaatttc taatgcttcc tgttttgaaa gaacacggcg
     2641 ttcgatcggc agattttctt tgacgatttt catcatttct gcttcgattg ccggcaaatc
     2701 ttctgcagtt acttggtttt gtccattatc tgtatcgtag taaaaccctg aatcgatagc
     2761 tggtccaacg ccaaagtgga tatttggata gaggcgacgc atggcttggg ccatcaaatg
     2821 agctgctgaa tgacggacta agccaagtgc atcttcatgg tctggtgtga tgatctcaat
     2881 acttgcatct tcctcgattg gacgagtcaa atcaacgagt tttccgttca ctttaccagc
     2941 taaagctttt tttgctaagc tttttgaaat gctttcagca attgccaatg ttgttgttcc
     3001 agactcaaat tctttgactg caccatctgg aaaagtaatt tttatcataa tagtttcctc
     3061 cttgaaatgt tttgattgat cagatcatca agctgaaaaa caaaaaaatc ctagcatctc
     3121 ttgaaaagag atactaggac gaaaagttcg tggttccacc taatgttcaa acgcaaatgc
     3181 gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca acaagggttt cgaaagtggt
     3241 catcctttgg ttttctcctg gaaaagtttc agcctagctt ttcctctctt atcgaagact
     3301 ctcaaattca gttggctttc tcatcaatca attatgtgtt tatttaacca ctaataaata
     3361 gaaaaatcaa gtgggaaact aaacttattt tttccattcc ccatttacac cgtatgttcc
     3421 ttttttcatc tcattgatga tgacatggat gtgttctttt ggtgcctgcg tgtttttatg
     3481 aacagcttcc gtgatatctt tcatcatatt ttctaattgt tccggactgc gaccttctac
     3541 taattcgaca tgtacgaatg gcataatgct ctctcctctt tctgatcgtt cttttttcaa
     3601 ctcgattcta tcatactatg tttgtcttgg gtactgaatc aggaaaaatg gctggatctt
     3661 tttttgctct caaggcactc aaaatagaga ccgtatccac gacttcttgc aaaacagctc
     3721 cgaataatgc ggggattagt cccgtacttg caattaacat caaaaaaata caaataaaga
     3781 tgccaatcag tacagactgt ttcgctattc tcatcgtatc tcttgaaatc actactgctt
     3841 ttgctacacg caagagatca tctttcaaga tgaccgcatc tgcactttca ctagcagctg
     3901 tagaaccatg agcccccatt gcaataccga tatctgcagc agcaagtgct ggtgcgtcat
     3961 tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt agggatactt tctaaaatgg
     4021 tcagtttatc ttgaggcaag ctacgggcat gtacagaatc taattggact ttttctgcaa
     4081 tcgtttgagc aatggcagat ccatccccgg tgatcatcat caaatttttc acatcaagtt
     4141 ttcttagctc cttgattgtc tttgctgctt ctgggcgaat ctgatccgaa aataggatcg
     4201 ttcctgcaaa agatcgatca attgttacat agattgctgt attggacgtt tctatttctt
     4261 cagctgaaac aaaagaggca cgtccaatct ttacttcatg attattgact actgcttgga
     4321 ttccttgacc ggtcacttct ttcagttgac taacaggata cagttggctg tttttcacgt
     4381 tgttcactaa cgaacgagct aagatatggc tagattcctg ttctgcacta gctgcataca
     4441 gaagtagctc ttcttttttt attccattga ctggctgaat cgcctctact tccaatatcc
     4501 ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt tgtacgtgcg agcttttcaa
     4561 tcgttgttcc agtttttaca acgatcccgt ttttacttga acggctcatt cctgcaacta
     4621 aagcaatggg cgcagctaga atcagtggac aaggtgatgc cactaccagt acctcagcaa
     4681 atcgtaaagg gtcttttgag aaccaccaag aaataccacc gatcaaataa gcaatcaacg
     4741 tgaaagggac tgcataacga tcagctaaac ggacaaatcg ggcaggcttt tcttttgatt
     4801 cttcgactag tttaataatc atttgatact ggctgtctgc ggcacgtttt tcagcgatca
     4861 tttggatcga tccgtctcca tttaaagacc ctgacataat ctgatctcca ggtttcttgg
     4921 tttccgggcg ggactcacct gtcaaagaag attcatcgac cgaagattct cctttggtca
     4981 ctcgaccatc tgcgggtaca agttcgccag gcttgactag taactggtca tcaatattta
     5041 tttcatcgat tactatatcc tgcaattttc cttctttgaa tcgatgggca attcttgggg
     5101 tgttatctaa caaagactgc agttctttac ccgctcgttt attcgcgtaa tcctctagac
     5161 tatctccgcc cgtaagcatg atcaatatca tcaaactagc ccagtattct ttgacggcta
     5221 atgttgcaag gattgcggta atcgctaata catctacgcc atatcttcct gaacgtaacg
     5281 tcttgatcat ttccaaaaac atcaagaagg cgagaatccc tccagtaaat gcgacaatcg
     5341 caaatgcaag ggccggctgg tgaaaaacaa attcacttaa taaagctatc ccaccaataa
     5401 gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt ttctcctctt ttatgtgact
     5461 tagagaggtt agtaataaat ttccagctgc ctctttcatc ttaagcatag tccttgacta
     5521 gcacttttta aaacaatagg ccttaattga aaacaactat cactatcagc gtttccaaaa
     5581 ttcatcagtt ctacaaacaa aaatattcag caaggattac tttttcgatt catcagttaa
     5641 aaagtacctt ttttcgaaaa actatgctat acttttaatt cgttatttac ggcggaagga
     5701 gcaaacatgc gatgaatgag aaacaactag aacaaacgca cgtggatgaa acgatcgaac
     5761 taatccagat cgaacaaaat atgctggata caaaacaaca agaattgact caggaaatga
     5821 atcattcgac aaaagacaca gctaatcaca agattcgagc tggttccaat gaagcttttt
     5881 atgaatctgc tgttgaatat cgccaacatg agcaggaatt gcttttgaaa taccatactc
     5941 ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat gaaagatagt ccttattttg
     6001 cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt atatctaggt atcgcttctt
     6061 taagagatct ggcagaagaa acaatcgtta ttgactggcg cgcaccaatt gccaatcttt
     6121 attatgaagg tgagattggt cccgcatttt atgaaactga aacagataga atagatgtcg
     6181 atctgcagtt gaaacgacaa tttaaaatcc aagaaggacg tattttatct atggtagata
     6241 cgtcggaagt aatcaatgat gactttttgc tagagatatt ggatgaatca tcaagcaacc
     6301 atatgaaaaa catcgtctct acgatacaaa aagcacaaaa tgcaatcatt cgggacacaa
     6361 caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg aaagacatcc gctttgcttc
     6421 aacgtattgc ttttctgttg tatcataatc gcaagtggct cgaggctgaa aacgttcttc
     6481 tattttcacc caaccatttg ttttctgatt acatctcgac tgtattgcct tcattaggtg
     6541 aaagcggcgt gcctacacag acattcaaaa attatttggt gcagctgctg ccagagtttt
     6601 ctttactaga tgaacagcaa caagaaagtg gttttttgct aggggaaaaa gatccgatac
     6661 aagtgatgaa aagcggattg accttagtcg atcagattga tcgctatatc caaggcatca
     6721 caaactacgg tcccttgttc cgtgatatga aaataaatgg ccgtacgatc ctatcaaagg
     6781 aatcgattcg ccaatggtat aaagaaacaa atgaacaatt acctttacat catagacttt
     6841 ctctattaca aacgaagctc ctgaaaaaat taggcggtct gcaaaaagac gaaacacgtc
     6901 agcaatgggt caaagatctg gcggaagaac agctccaaga actatacgca acagatccaa
     6961 atcttgaata tacagaacaa aaggagcgtt cattacgtaa aaaactggcc aaccaaatcg
     7021 tacgtaaaag attccgtcga atccatcgag ggatcatgaa ttatcaattt gtcaatataa
     7081 cgaaacaata tctccacttt ctacaaactg tgccactttc tcttctaaca cgacataaaa
     7141 tcagcgagac caggtggcag gaacatttga caactgttcg tacacacttg cgtcaaagag
     7201 aattgcgtca agaggatgct gtactattct ttttactgat gcgccgaaca tatccagtga
     7261 cggttacaca caaagctcgc tatatcttta ttgatgaaat gcaggatttc gcaccatcac
     7321 aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt gactttctgt ggagatctaa
     7381 atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt ggaagacctt ttcgtggacc
     7441 gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc cacaaaagaa atcactgatt
     7501 ttgctaatca ttttattgtg aaagaacatc aagtagaaac aacagcaaga aaaggagacc
     7561 ttcctttgct catccaagga acaacagatg atatcaaatt gaattggtta gcagatacca
     7621 ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc aatcatagct aaaacacaag
     7681 aagaatgcga agctctttat gctcaactgc cagacgagtt gaaacaacaa gtccagctga
     7741 ttttaaatga gagtgacttt atgaagcgtt ccattatttt acttccagcc tttttagcaa
     7801 aaggtctgga gtttgaccga gttttcttgt gggatgtaga tgcagaacat ttttcttctc
     7861 agcaagacaa attgatcctt tacaccatgt gtacacgtgc gatgcatgaa ttgacccttt
     7921 ttacctccaa taatgaaagt cctttgttaa acaatatgga tatttcatct tatgagaaaa
     7981 aagtactgta agtaaaaaga caactgctat cattaatgtt agcagttgtc tttttattta
     8041 caataataat tgcagaaaaa gtaattcaat catttaaggt catttattct atatataaat
     8101 ccaaatctgt ttcaggatat tttaatttga caagcgaaat aatcattttc agttcttcca
     8161 tgttttctgg atattctggg tcaaatacct tgctatgacg gccaatcccg ccattgtcga
     8221 tcaatcggta aattcgattc cattcaatga attggtcaat tgattggaaa ttgctgattg
     8281 gatagccgcc acaatccaaa atctctgata cacgaaatgc taggtatgca ctatcgatca
     8341 ctttattttt tttcgtgtct atcttctcat ttctttcgat aagaattttt tcaggacgat
     8401 gtttcaagct ccactctaat agtagaaaga aagcctccat ccgatactct tcttcaaata
     8461 accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat cagataattt tgtctataaa
     8521 tcagcattgg tgcgcggtcc accatacact ctaaataacg gttcagatac cctgtaccga
     8581 catagtttcc taatcgatag ccttcaacgc cgaatcttgc gtatttttgc gctaattttg
     8641 aacaatcagt tacgttcaca gctaactctt ctactttgcg gtaaccgctt aagatacgtt
     8701 caaaaggttc taaagtgatt tttgcttcca tattcttccc cgtccatctt ccacatactt
     8761 catatcattt cacatgatac ttatgctctc atcataccac aggttgaatt agaaaagaaa
     8821 ataaatcaca caaaaaagca agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag
     8881 actgaatact tttcgttaaa actggttgct tagtcttcta atgccacatt atggaacaca
     8941 tgctgtacat cttctagatc ttccagtgca tcaatcattt tttcaaatgt ttcttgatct
     9001 tctggagata gtgttacctc gttttgaggg atcatttgga tctcagcgat tgaaaaatca
     9061 gtgattcctt tttcttttaa tgcttcttgt atactatgga aatccgctac ttcaccgtaa
     9121 acgatgattt gtccttcttc ttccattaca tcgcgaacat cgatgtcttt ttccattagg
     9181 tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa agattgctgt attgtcaaac
     9241 atgtaagaaa ccgctccgga aacacccatg ttcccgccat ttttcccaaa tgctgcccgt
     9301 acgtctgctg ctgttcgatt cacattgttc gtcaatgtat caacaatcac cattgagccg
     9361 cttggtccaa atccttcata gcgcaattca gagtatgttt catctcctgc accttttgct
     9421 ttttcgattg ctcggtcaat aatatgtttt ggtacattat aagttttagc gcgatcaatg
     9481 acgaaacgta gtttttggtt tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag
     9541 atttcaatcc caaattttgc ataaatccgg ctattattag catctttagc agttttcttt
     9601 gctacgatat ttgcccattt acgtcccata ttttcacttc gctttctgat ttgatagttt
     9661 cataacgcaa aaacgtcctg ttccattata cttgtgaatc acgattctgt gaagcttttt
     9721 ttctttcttc cttcattctt ctcattttta gtcaattatc tatactcgga ttgggatttg
     9781 cccgaaattc tgttccggaa tattgcctcc caattgatag atttgatcac ttctaactaa
     9841 cagttccgct tcttgtgcct ctttttttcc gattcgggaa taaatagaaa tctctttttt
     9901 ttcttttaag aatcttcttc cagcatcggt aaatccaagt agtcgaagat aatttttatt
     9961 ttgttggaca ggaatctctt gctttttgat attcagcaag acataacaag ataatcgttg
    10021 gatccgtgtc catgtatatc tttttgtttt tacctttgac acaaaatcct caaaactatc
    10081 agcagttgca gctgctgttt tcatgcgcga ttctattcct tcggtcatct gatagatatc
    10141 tgtcagttcc agcaaagtag aagataacaa ccgataacgc aaatacggcc aataagattc
    10201 ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc tgaggcggca caagtccagc
    10261 tattcttttt ccttcttcta acgcatggcg aatggctgtt gcactggcta tcctctgatc
    10321 tgtgatgatt ttttcatgat agcctgcact tttacgtcgg atcggatgta attccatagg
    10381 gtggacatac ttggcatttt cctttgcata acttaatccc aagatatgat ttggcgaaga
    10441 aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc tcagtcattt tttgcggata
    10501 actatacgtt tgatcagaca gttcttgaaa tgcctcttct atcctatctt gattctcttg
    10561 gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa
    10621 actgtcacag tccagtgttt gcaagatttt tactgcacct ttagcaaaat agtcagctga
    10681 ctgaaccgcc cattcgacgg gaagctccac tactaaatcc acaccatttt gtaaagcttc
    10741 attcgcgcga tgccatttat cgatgatagc cggttcgcct ctttgcaaaa aattcccgct
    10801 cattacagca atgacgacct ctgctcctgt tatttttcgt gccatttcca catgatagcg
    10861 atgtccattg tgaaaaggat tgtactctac gataatcccg cagcttttca tgcgcatcct
    10921 ccttcatagt tatcgataaa aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa
    10981 aaatacccga actatataaa ctattctcgc aaacacaata cttctgcgac gaaaagctgc
    11041 actgcaagag caatgcctat atcgttcgga tatataattt atgttgaaga tttattcttt
    11101 tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat gctacaaaga accagcgctt
    11161 tgatttctct gatgctgctt cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc
    11221 gctgttttcc agcattctta aataactttc caatggatag gtacgctctt gatgtagttc
    11281 atcttcacga ataaatcgac cagacccttc ttgttcttca acgaaaaagg taagaaaatg
    11341 ttcgatgctg tgtttctcgc ttcctggata actatcccac aaaaaagcaa aattatcccc
    11401 ttgataatga tagctatatt ctggaaaaac ctcatctact tgataggtgg agtgtacgtc
    11461 aaataaaaat cggccatttt cttctaacgc ttgatagact tcatcaaaca cttgctgtac
    11521 ctcttgtcta tttttcatat agcacagcga atcagaaaaa caggtaattg cctgatattg
    11581 tccgatttct gataaatcga gcatattgcc ttccacaaat tggatcgatg cgtcttcttc
    11641 atatgctcgc tggctggcaa tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt
    11701 atatccatct tttgcaaagg caaccgctaa tgctccagta ccgcatgcca gttccaaaat
    11761 attttttgtg tcttcttgta aatgacgttt cgaaaaagct aaccattgct gataaagtgt
    11821 ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca taagccatat gttaatccaa
    11881 ccattcacta agatcgacta gcggagcatc tgaccatagt ttttccagat tatagaaatt
    11941 tcgttctggc gcatggaaaa catgtacgat cacatctcca agatcgatca atacccattt
    12001 tccgccttct tttccttcaa tacgttttac ttcatatttg ttttcttctt ctttatcaat
    12061 gatttcttct gtaattgcgt tgatttgacg ttcgctattt gctgaacaga tcatgaaata
    12121 atctgccaac aaggaaacat ttcgaacgtc taaagcgata atgtcttctg cccgttttga
    12181 atctgctgct tcaatcgcaa tttttaattg ttctttacta tctatgatga tttcctccta
    12241 atatgagcag cttatttttt tgctacccag taattatatg tttcgattgt ttttggataa
    12301 atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct
    12361 tcgtctagat cagcccatgc aatggctcgt gcatcttgta ccccaggaaa atctcgacct
    12421 ggttcaatat agtctgctac ataaatgatt ttgtcaagga gactcatctt agcagcccca
    12481 gtcgtatgca cccggattgc atgaagaatc tctgcatcgg tgattcctaa ttcccgctct
    12541 acaaacgaag cgccaacaag accatgccag attgcattat tataattcaa taactccgga
    12601 tcaaaaccgt ctcttacgat taccattttg aactcctcat ctggtcgttc ttttgcataa
    12661 tcatgtgtca atgcagcaat actagctttt tcaggagaag caccgtattt tttggcaaga
    12721 gccacggctg tttcttctac tccaagcaca tgcttgaagc gttgttcgct cattctcatc
    12781 tgtatttttt gcatcaggtt ttctctagaa taagcagtat attcattact atagtccata
    12841 ttcatacaac ccttcatttt gaatataatc aattacttta tctggaacaa ggtagcggat
    12901 agaacagcct tctttgatct tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac
    12961 atctacccaa attacaggat atggagtatc cgttgtgtat cctggtcgac gtatacccac
    13021 aaagttgacc atagaagtca gctcatcgat tttataccat tttggcaaat attctaccat
    13081 atccccaccg ataataaagt aataatctgt atccggattg ttttgcgtca attccttcat
    13141 agtatcatag gtgtaacttt tcccaccacg agctaattca atcgtttcaa tctgcaaaaa
    13201 aggattgtct tccaccgcta attccaacat gttgagtcga tgttttgcat caatcgtctg
    13261 tttctcatca acatgaggtg gctgataaga aggcatcaaa aatacgcggt caagacctaa
    13321 attccggcca gcttgttcag ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc
    13381 gcccaagatc cctacttgct ttttcttctc taagaattga ggcatttctt caggaaataa
    13441 ttgtgaacga acaaatgtct tagcttgcgt attcatcatg gtcactcctt atattgcttt
    13501 tacttctttt gatagccttt gatacttttc ttttgtggaa ggtttgtata atacaataac
    13561 acgtccaatg atttggacga tttcgcaatc tatttctttt tctaatactt gagctgcttc
    13621 ttctgcaatc tcatctgtat tttgaagtaa cgatactttg atcagttctc ttttttctaa
    13681 agcttcttca atttgaacga tcattgctga attgatccca ccttttccga tttgaaaaat
    13741 cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat
    13801 aattttcctt cttccttaaa tcaatgcttt gcgtttcaat acatctacac cctttggcgc
    13861 ccagcccgca attactactg gcgctgttac agtgatccag cctagaccgg caaaaacgat
    13921 gtctgttttt tctttcacag aaaactcaaa acgaacaagc tccggaaatg cctcgacttc
    13981 atcaggacga ggaggctgta ataatccacc tacatgtttt tgataaaatt cgtctgcttt
    14041 ttccagtttc gttcgatgaa tatttacatc atttgacaca taagcaataa atgaagcacg
    14101 attgccgctg ataaaatcaa atcttgcaag tccgcccaag aaaagtgttt gcccttcatt
    14161 cagctgataa actttaggtt tgatttcttt taacggtgca atgatccgca aatctttttt
    14221 acctaagtag tgcgccattt gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg
    14281 cccgtcatct aaaggaatct cgattttatc caaggtcgtt cctggaaact gcgaagttgt
    14341 gattacatct tgtactcccg ctgtattttt gatgatttga ttgataagcg tcgattttcc
    14401 gacatttgtt actccgacga tatagacatc ccgtccatcg cggtattctt cgattttttc
    14461 taatagttcc gtcatctcat gagatttctt agcactagtt aataatacat ctactggacg
    14521 aattcctgct tcgtaagcac gttccttcat ccattgtgtt aatttagttt ttttcaatga
    14581 tttaggtaat atatccactt tgttcccaac taaaagtact ggattgtcac cgacaaaacg
    14641 gtgtagtcct gggattagtg aaccattgaa gtcaaaaata tccaccacat tgacgatcaa
    14701 tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac
    14761 atcttgggtc tcattataat ggcgtaatct gaaacaacgc tgacaataga cttccccgga
    14821 agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt
    14881 ttgtataacg gcaccacagc cgatacattg taattcttct gtcattcgat tccacctcgc
    14941 cagcccatat cagggtgttt tgctaataga taggccataa ttcttttttc ccaaaaacga
    15001 ttaaattgtg tcttccaact atctgttgcg ataatgggtt ttacaaggat gctacgtatc
    15061 cctgcacgat tggctccacg tatatcagtc ataatctgat cgcctaccat tactatctca
    15121 tcatctgcta aatccaattg tttctttgct atgttgatcc ctcttgctaa aggtttcatt
    15181 gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta
    15241 ttgtttgaaa caacaacaac agggattcct gcatttttca tttctaaaat ccaagtaagc
    15301 aactcttctg ttccatctgg attattccat gcaatcaacg tattgtctaa atcggtcaat
    15361 actgctttga tgcctagatt ctttaattgg gcaggtgtaa tctgatagat ggcttctacc
    15421 atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag
    15481 cacataaagt actcctcaac cccgtattat actgcatatc agcaaaaaaa actacctcta
    15541 tactcgttcg tctttcagaa aaagcatttc tttacaaaat gaccaaaaat aaagattgat
    15601 cacagaaaaa tttagaaaca aaaaacaact aatattggcg gaacctcttg tatcgttttt
    15661 ccagtaattt atgctgatct aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt
    15721 tcataatgtg tgcgacttta tatcgtggca actgctcgcc gttgaacgtt tcaggaataa
    15781 ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt aattttcatc aactcagctg
    15841 cttctgcggc gcgactaccg tctttccaca aaatcgacgc aaatccttct ggagaaagca
    15901 cagcataaat cgtttcttcc agcatccaga cttcatccgc taaacctaga gcaagggcac
    15961 cgccacttcc accttcacca atgatgatgg cgatgatcgg tacctttaaa tcagacattt
    16021 cgagcagatt tctggcaata gcctctcctt cgccgcgctc ttctgctcct acaccgcaat
    16081 aagcacctgc tgtattgatc aaggtaatca ccggtctatt gaacttttcc gcttgtttca
    16141 tcagtcgtaa agcttttcga tagccttcag gatggggaga accaaaattt ctgcggatat
    16201 tttcctgcag atcccgtcct ttttgaatac cgatcactgt taccggtttg ccatctaatc
    16261 gagcgattcc accaacaatc gcttcgtcat ctgcaaataa acgatcacca tggcattcct
    16321 cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg
    16381 ctaactgaac gacatcatgt gctgtcttat ccacgatcta tccatccttt ctgtgtatgc
    16441 aattcaagaa gcgtatggat tttctgtttc aattccatgc ggggaacaat ctgatcgaca
    16501 aaaccatgag ataataagaa ttcagccttt tggaaatctt ctggaagttc ctgcttgatc
    16561 gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc tttggggttc tgctagaata
    16621 atatcgcctt ccattgcaaa actggcagtt actcctccgg tcgtcgggtc cgttaaaaca
    16681 gttagataaa aaagaccttc attgctatgc cgtttgacag cagcagagat ttttgccatc
    16741 tgcattagag aaaagattcc ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg
    16801 actggaagac tttcctttgt tgctcgttcg aataatctgg tgatcttttc tccgacaatc
    16861 gttcccatac ttcccatgat aaaattagga tccatcactc caataatgaa gggaatcccc
    16921 tgaatggttg ctttaccagt gaggactgct tcatgaagtc ctgttttatc ttgcattttt
    16981 ttaatttttt ccgtatattc gggaaatgct agcggatctt tggttacaag atccgtatcc
    17041 cattcttcaa aacttttttc atcaataatc aaagctagac gctgccatgc accgattcga
    17101 aaagcgtatc cgcaatgagg gcatactttt tcttcaccga tgtctttcgt ataaaggata
    17161 tgtttgcaat ttggacactt tgcccacatg ttatcaggaa cagatggcga gttagcaggt
    17221 ttgctctctc tattaggatt gatccgaata tatttctttt tcttaaacag agccatctat
    17281 gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct
    17341 gtgctgtaat cacctgctat gactgcagga tgactaatca aatccatctg aaattctgcg
    17401 ttcgtgatga ttccttctgt gataaattca cttaaggctc gctgcatctt cattaatgct
    17461 tcaaaacgcg tatttccatg gacgattatt tttgcaatca ttgaatcata atatggcggg
    17521 atcgtatatc ccgggtaaac tgcactgtct actcgcagtc ccatcccacc tgctggaagc
    17581 agtaagtttt ggatcttccc tggagaagga gcaaaatgga aagcaggatt ttctgcatta
    17641 atccggcatt cgattgcgtg tccttggaat actacttctt cttgacacac attcaaaggc
    17701 tcaccggctg ctatctccac ctgttttttt actaaatcga ttcctgtgac catttctgtt
    17761 actggatgtt ccacttggat tcgagtattc atttccatga aataaaaatc gccttcttca
    17821 tccatcagaa actcgatcgt tccggcattt tcataattga ctgctttagc tgctcgaaca
    17881 gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca ctacagaagg agattcttcc
    17941 agtacttttt gattgttgcg ttgtaaagaa caatctcgct cgcctaaatg aatgacatga
    18001 ccaaatcgat cacctaagat ctgaacttct atatggcgag caggataaat gattttttcc
    18061 aaatacatat catcatttcc aaatgccgct gctgcttctt gctgagctga agaaaaatgc
    18121 tgtgggagct cttcttttga ttgaactttc cgaatgcctt tcccgccacc acctgcagct
    18181 gctttcagca tcactggata accaattctg tctgctattt ccaatgcttc ttctactgta
    18241 gataatacac cagtacttcc tggaatgact gggacatttg cttcttgcat caattgacgc
    18301 gcattgatct tatttcccat tgcatcgatc gttgcacttt taggaccaat aaatgtgata
    18361 ttgcactctt cacacatgga ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga
    18421 tgaattgctt cggcttttgt cacgattgca gcgctcagta cttcctgaac attcagatat
    18481 gaatcaacgg cttttgccgg gccaatgcag attgcttcat ctgctaactg tgtatgaagt
    18541 gcttcggcat ctgcttctga atatatcgct acggtttgga tccctagttc gcggcatgca
    18601 cgaatgatac gtacagcgat ttcaccgcgg ttagctatca atactttaga aaacatattt
    18661 cacctatcca atcataaagg ttaattccgc ttcggctact tttttaccgt ctactttagc
    18721 gattcctttg cctattccgg ctgaagcttt cactttcaat atctcaactt caaggtataa
    18781 ggtatctccc ggtgtaactt tcttacggaa ttttgctttg tccaatccgc cgaaataagc
    18841 cgtctttccc ttgaattgtt caagagaaag taatgcaact gctcctgctt gagccattgc
    18901 ttcaacaatc aatacccctg gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg
    18961 ctcattgatc gttacatttt tcttagcaac gatccgttct ccttcaacca tttcttccac
    19021 tcgatcaatc aaaagcattg gataacgatg tggaatgatt tcttttattt cttgtatgtt
    19081 cattgttttc cctctttcct gctttgctta tttttgctga atcctaaaga gtggctgtcc
    19141 atattcgaca actgactcgt tttctacaag gatttctgtg atcactccgt cggcctcact
    19201 agttatctca ttcatcagtt tcatcgcttc aataatgcag ataacttctc ctttggcaac
    19261 ttgatcacct acttttttga aagctggttt gtcgggcgct ggttgcagat aaacgattcc
    19321 cacgattggc gaaacaacct cgattccatc aaatactgcc gattcttttt ctttcattat
    19381 ttctggagca gttgttgctt gttcaggtat gacattgact ggctccgaaa ctggcaacgt
    19441 ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta tttttattca tatacaattc
    19501 aaattgacct tcacgcaaat cgaattccgt tagcgaggat tgatcaaatt gactgactaa
    19561 ttctctgatt tcattgatat ccaaaattat tcctcccagc gtttcatgca cagaaccgca
    19621 ttatgtccgc caaaccctaa tgaattactc attgcataag taaaacttgc aggtttgctt
    19681 tcgtttttga caactgtcaa gtcaatctca ggatcttgtt tttctacatt gatcgtaggt
    19741 gggataaatt gatgttctaa tgccagcaaa gttgccaagg cttcgatccc gccggctgct
    19801 cccaataaat gtccagtcat cgatttcgtg ctgctgacgt aagtatctct gaaattttca
    19861 cctaatgcga tttggattgc ttttgcttct gcctggtcat tggctggtgt acttgttccg
    19921 tgagcattga tatatccgac ttgttccggc tgtacaccag cttcttccat cgcaagcttc
    19981 attgctttag atgcaccaga accatctgga gtaggagctg tcatatggta agcgtcacag
    20041 tttgcaccgt atccaacgat ttctccatag attttggcac cgcgagcttg tgcatgttct
    20101 aaagattcaa gaactagaac gcctgctcct tcacccatca caaaaccatt ccgattttcg
    20161 tcaaacggaa tcgaaccacg attaggatca gttgattctg agagtgctgt taaagctgca
    20221 aatccagcaa tccccatctc acaaatcgtt gcttctgttc ctccagcaag aatgacatca
    20281 gaatagccat gtttgatgtt acggaatgct tccccaattg aattcgttcc gctagcacaa
    20341 gctgttacga tagctgtaca aatacctttt gcacctacac gcaaagcaat atttccagct
    20401 gccatgttgg caatcgccat tggtacgaac attggagaaa ctcgttttgc acctttttca
    20461 tgtagacgaa taacttggtt ttcgattgtt ggaagaccac cgatcccaga tccgatgatg
    20521 acgccaaacc ggtcttgatt cattttttct ttatctaaac cgctcatctc cattgcttct
    20581 aaagcagcgt agattccata gatcgaaaac atatccattc gtttgctatc ttttttaata
    20641 aaatatttat cgtaagggaa atctttgact tcacccgcta aggtaatccc tgtttctgaa
    20701 gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct cttttaagct attcaaaaag
    20761 ctgtctgcgt catttccaat aggtgaagtg actccatagc cagtaattac cactcgattc
    20821 atttgtttgc tctccttttc ttatccgtgc atgaccaatc cgccatcgac attgatgact
    20881 tgtccggtga tatatggatt ttgtgctaag aaaactgctg tttgtgcaat atcttctact
    20941 tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa
    21001 acttccgtca tatcagttgt gatgaatcct ggcgcaatgg cattacatgt aatgcctcta
    21061 gttgcagctt ctcgtgccac agattttgtt aatcctacta cgccagcttt actagctgca
    21121 taatttgctt gaccaatatt tccaatcaac ccagatacac tagagagatt aatgatggcc
    21181 ccttctcgct gcttcatcat ttttttcaat acgtgctgcg tcatattgaa ggttccaatc
    21241 aaattgatat ccagacattt tttgaaatct tctgcatcca ttcgcatcac aagtttgtca
    21301 tttgtgattc cggcattgtt tactaacaca tggatcgatc caagcttttc ttctgcttct
    21361 ttgatcattt gtcccgcttt ctcataatca gagatgtcac cggaaactcc tacgcatttt
    21421 acaccgaatg cttcgatttc ttctatcttt tcttttggaa tctctccgcg accattcaaa
    21481 atgatatttg ctccagcttt tgcaaaagct aaggccatcg ctttgccgat tcctcttgta
    21541 ctacctgtga taaatacatt ttttcctttt acatccataa cgttcctcct tatagtattg
    21601 ctattgtttc atcaaatgtc tgcttattct caacacgcag aactggtatt gttttatcga
    21661 tttttttcat aaatccgctt aatacttttc cggggccgac ttcgatgact tgttcgatgc
    21721 cgattgtttt taacttatgg atactctcat agaaacgaac aggttgcatg acttgctgtt
    21781 caagtagtcc cgcgattgtt tctttcttca ttatttccgt tgttgtgttg cttatgatag
    21841 gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt cgccagtttt tttgctgccg
    21901 gttctaaaat agctgtatga aaaggcccgc tgacatttag agggatcatt cttttgaacc
    21961 ctttttcttt taatagtaaa acagcttcgt ctactgcttt ttcttcgcca ccaatcacga
    22021 tctgctgagg tgtattataa tttgcaggag atacgatgcc gtacttgcta gcttcatgac
    22081 agctttcctc gatcgtttca atcggcgcat tcatcactgc aaccattttc ccgcttccag
    22141 ctggggcagc ttctgtcata taagcgcctc tttttgctac taacgcaact gcttcagtga
    22201 aggatagcgc attactagct actaatgccg aatattctcc taaactcagt cctaaagctg
    22261 catctgggat gattccatgc tccttcaata agcgataata cgcgatgctg actgttagaa
    22321 tagctggctg tgtatattgc gtctgatcca aacgttcatt ttctgtaaag caaagttcag
    22381 ccatatcgta gccgagaatc tcacttgctt catcaaatgt ctgctttaca acggcttctt
    22441 catataaatc ttttcccata ccttgatatt gtgcaccttg gccactaaat aagaatgctg
    22501 ttttcatgtt tttctccaat atatccagaa tatatgtttg attcttccta ttcaaaaagg
    22561 ccgagacatt cctatttcag gaaatgtccc agcccccaac ctaattttaa tgattatgca
    22621 gtttgttttt ctacgtaagt cactaaatct tgaaccgttt tgattccgtc ttctgtttcg
    22681 attttcacgt caaattcatc ttcaatttca ttgatgattt ggaataaatc caagctgtca
    22741 gcttctaagt cttcttggat gtcagtagtc aatttgattt cttcagtttc ttttcctaat
    22801 tcttctgcga tgatttcttg aattttttcg aataccataa taatatattc ctccgatatt
    22861 gtttgctttt tatttttata atttaacagt ctacaactcc atgagaatac ttccccatgt
    22921 taaacctccg ccatagcctg taaatattac tttctgctgt tttcctaagg tgatctttcc
    22981 actagaaact gcttcatcca ataagatagg gatgcttgca gcagatgtat tcccatattt
    23041 atccatattg gtcaggaact tttcttgcgg catctttact tttcgggcaa ttttctcaat
    23101 aattctgagg tttgcctgat gcaaaagcag ataatcaact ggtttgtctt tcatgacttc
    23161 ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg
    23221 caaataatac gaaccttctg aatcttctga atagaacgga tttttatttt gctggtagcc
    23281 agcggtcaat gagctacttc tggtaccgtc agctgctaat ttttctttca gcaatttttt
    23341 ctgtggattc gcttctaaga gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt
    23401 acgatcagac caatcgatta gcttagatag tgtttcacca ccaatcacca gtcccttagt
    23461 tgagccgcag cggatcattt tttcagccat gcttaacgca taaacaaatc ctgagcacgc
    23521 agcactgata tcaaaagcga tagcatgagt tgctcccaat ttcccttgta cttgtgctgc
    23581 tacagatggt gtcgtgtaat cgggcgacat tgttgctact aaaataaaat ccaaatcttt
    23641 agggtttaga tttttctgtt ttatcaattt ttccgccact aaaatgcata aatcagaagt
    23701 ctcctgattt gttacacatc ttcgttcacg aatacccgtc cttgacgaaa tccattcatc
    23761 agaggtatcc atcatcttgg aaagatcatt attagtaata atcttttcag gaaggacgct
    23821 ggctgttgca gtaatcattc caaattgttc cattaaatat cctcacttat agtcttgcaa
    23881 gaagtcgtgc aagtttttca gtgcatccag caaggcatgc tgttcctccg tagccattcc
    23941 gtctaagatg ttcttgacca tttcacgatg aaaatgctga tggacccgaa atagtaactt
    24001 tccttttttc gttagtccca actttacaac gcggcggtca tcttcactgc ggatacgttc
    24061 gacatagcct ttttttacta acttgttaat agcagtggtc aaggtcccaa cagtaattga
    24121 tagttctttg gctacttctg aagttgtttt tttcttgtac atcccgattg cctcgatcgt
    24181 gtgcatctct gtgattgaca gatctttgaa ttgagatttt ttcagctcag actcttcgat
    24241 tgtcaaaata tcattgaaga cactaacaag ataatcattt actgttttta aatctggttc
    24301 catgttccac cgtccactaa ttagtttgat tatcaaatct tttgatgttc aaactatatc
    24361 ttaacttttt tcaaaatgca accttaaaag atcatttttt tactatatga gctt
//
LOCUS       DAYYNQ010000040        24267 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000040 DAYYNQ010000000
VERSION     DAYYNQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.040"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..540
                     /locus_tag="ACHBJ1_001436"
     CDS             19..540
                     /locus_tag="ACHBJ1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430385.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            637..858
                     /locus_tag="ACHBJ1_001437"
     CDS             637..858
                     /locus_tag="ACHBJ1_001437"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9430386.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1015..1179)
                     /locus_tag="ACHBJ1_001438"
     CDS             complement(1015..1179)
                     /locus_tag="ACHBJ1_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFM9430387.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="ACHBJ1_001439"
     CDS             1282..1803
                     /locus_tag="ACHBJ1_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM9430388.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2230
                     /locus_tag="ACHBJ1_001440"
                     /pseudo
     CDS             1790..2230
                     /locus_tag="ACHBJ1_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(2270..2428)
                     /locus_tag="ACHBJ1_001441"
     CDS             complement(2270..2428)
                     /locus_tag="ACHBJ1_001441"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFM9430389.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="ACHBJ1_001442"
     CDS             2521..3282
                     /locus_tag="ACHBJ1_001442"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9430390.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3469..4821
                     /locus_tag="ACHBJ1_001443"
                     /pseudo
     CDS             3469..4821
                     /locus_tag="ACHBJ1_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            4837..6057
                     /locus_tag="ACHBJ1_001444"
     CDS             4837..6057
                     /locus_tag="ACHBJ1_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9430391.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="ACHBJ1_001445"
     CDS             6092..6769
                     /locus_tag="ACHBJ1_001445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM9430392.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="ACHBJ1_001446"
     CDS             6766..7962
                     /locus_tag="ACHBJ1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM9430393.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="ACHBJ1_001447"
     CDS             7977..8300
                     /locus_tag="ACHBJ1_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM9430394.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="ACHBJ1_001448"
     CDS             8304..8639
                     /locus_tag="ACHBJ1_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM9430395.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="ACHBJ1_001449"
     CDS             8636..9031
                     /locus_tag="ACHBJ1_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM9430396.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="ACHBJ1_001450"
     CDS             9028..9399
                     /locus_tag="ACHBJ1_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430397.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="ACHBJ1_001451"
     CDS             9415..9975
                     /locus_tag="ACHBJ1_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9430398.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="ACHBJ1_001452"
     CDS             10102..10413
                     /locus_tag="ACHBJ1_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430399.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="ACHBJ1_001453"
     CDS             10437..10628
                     /locus_tag="ACHBJ1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430400.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="ACHBJ1_001454"
     CDS             10628..14473
                     /locus_tag="ACHBJ1_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFM9430401.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="ACHBJ1_001455"
     CDS             14474..15346
                     /locus_tag="ACHBJ1_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFM9430402.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="ACHBJ1_001456"
     CDS             15343..16392
                     /locus_tag="ACHBJ1_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430403.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="ACHBJ1_001457"
     CDS             16396..17220
                     /locus_tag="ACHBJ1_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430404.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="ACHBJ1_001458"
     CDS             17238..19370
                     /locus_tag="ACHBJ1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM9430405.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="ACHBJ1_001459"
     CDS             19387..19536
                     /locus_tag="ACHBJ1_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430406.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="ACHBJ1_001460"
     CDS             19533..19979
                     /locus_tag="ACHBJ1_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430407.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="ACHBJ1_001461"
     CDS             19981..20118
                     /locus_tag="ACHBJ1_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9430408.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="ACHBJ1_001462"
     CDS             20155..20448
                     /locus_tag="ACHBJ1_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430409.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="ACHBJ1_001463"
     CDS             20471..20668
                     /locus_tag="ACHBJ1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9430410.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="ACHBJ1_001464"
     CDS             20679..21698
                     /locus_tag="ACHBJ1_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9430411.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="ACHBJ1_001465"
     CDS             21955..22602
                     /locus_tag="ACHBJ1_001465"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9430412.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="ACHBJ1_001466"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="ACHBJ1_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..24004
                     /locus_tag="ACHBJ1_001467"
     CDS             23312..24004
                     /locus_tag="ACHBJ1_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9430413.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
BASE COUNT         8545 a         4055 c         5273 g         6394 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa
    23701 tcaaaaaata gctgaaataa atcaattaaa cactgattgg aataatcgtt tacaaacaga
    23761 acaaaataaa tttaatgatg ctcaaaatac tgttaatagt ttaaatcaac aaattaatca
    23821 attgtcaaat gaaaaaaata ctattaaaaa ccaattgcaa agtactgaac aaaaactaga
    23881 aaaagcgcaa tcagaaaaca gtaatttgaa taattatatt gaaaaactgg aaaaagccaa
    23941 aagcgatgta gaagacacag caaacagatc acaagaaatc gtgactgaac atacaggaaa
    24001 atagatgcat tttttagttt aaatttcttt aacaaaatag tattttgttg ctaaataatt
    24061 tttatttgaa tattctacaa tgatatctcc cctcaaaatg gaggggaagt acatactgtg
    24121 ataatctaca ggtttattga ctgattagga tgttattaaa aaataaaata ctaaaatgca
    24181 tacccgaatt tcatacccga aaaataaaaa atgccaccac gtacactctt ttcgaaacta
    24241 cctaagtagc caagaatgta ctggtga
//
LOCUS       DAYYNQ010000041        24260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000041 DAYYNQ010000000
VERSION     DAYYNQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.041"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHBJ1_001468"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHBJ1_001468"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM9430414.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHBJ1_001469"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHBJ1_001469"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM9430415.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHBJ1_001470"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHBJ1_001470"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM9430416.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHBJ1_001471"
     CDS             complement(3373..4680)
                     /locus_tag="ACHBJ1_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM9430417.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHBJ1_001472"
     CDS             complement(4683..5264)
                     /locus_tag="ACHBJ1_001472"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM9430418.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHBJ1_001473"
     CDS             complement(5836..7128)
                     /locus_tag="ACHBJ1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9430419.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHBJ1_001474"
     CDS             complement(7125..7748)
                     /locus_tag="ACHBJ1_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430420.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHBJ1_001475"
     CDS             complement(7751..8587)
                     /locus_tag="ACHBJ1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430421.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHBJ1_001476"
     CDS             complement(8604..9491)
                     /locus_tag="ACHBJ1_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430422.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHBJ1_001477"
     CDS             complement(9507..10790)
                     /locus_tag="ACHBJ1_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430423.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHBJ1_001478"
     CDS             complement(11473..12156)
                     /locus_tag="ACHBJ1_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM9430424.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHBJ1_001479"
     CDS             complement(12174..12791)
                     /locus_tag="ACHBJ1_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9430425.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHBJ1_001480"
     CDS             complement(12801..13625)
                     /locus_tag="ACHBJ1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM9430426.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHBJ1_001481"
     CDS             complement(13618..14178)
                     /locus_tag="ACHBJ1_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430427.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHBJ1_001482"
     CDS             14352..15308
                     /locus_tag="ACHBJ1_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9430428.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHBJ1_001483"
     CDS             complement(15613..16569)
                     /locus_tag="ACHBJ1_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM9430429.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHBJ1_001484"
     CDS             16673..17275
                     /locus_tag="ACHBJ1_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9430430.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHBJ1_001485"
     CDS             complement(17363..18862)
                     /locus_tag="ACHBJ1_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM9430431.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHBJ1_001486"
     CDS             complement(18878..19402)
                     /locus_tag="ACHBJ1_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM9430432.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHBJ1_001487"
     CDS             19700..20449
                     /locus_tag="ACHBJ1_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM9430433.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHBJ1_001488"
     CDS             20440..21225
                     /locus_tag="ACHBJ1_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430434.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHBJ1_001489"
     CDS             21194..21910
                     /locus_tag="ACHBJ1_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9430435.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23031)
                     /locus_tag="ACHBJ1_001490"
     CDS             complement(22012..23031)
                     /locus_tag="ACHBJ1_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM9430436.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23050..24051)
                     /locus_tag="ACHBJ1_001491"
     CDS             complement(23050..24051)
                     /locus_tag="ACHBJ1_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM9430437.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5221 c         4225 g         7626 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DAYYNQ010000042        24005 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000042 DAYYNQ010000000
VERSION     DAYYNQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.042"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..448
                     /locus_tag="ACHBJ1_001492"
     CDS             131..448
                     /locus_tag="ACHBJ1_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9430438.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(539..904)
                     /locus_tag="ACHBJ1_001493"
     CDS             complement(539..904)
                     /locus_tag="ACHBJ1_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430439.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1112..1594
                     /locus_tag="ACHBJ1_001494"
     CDS             1112..1594
                     /locus_tag="ACHBJ1_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430440.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1791..2681
                     /locus_tag="ACHBJ1_001495"
     CDS             1791..2681
                     /locus_tag="ACHBJ1_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM9430441.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3007..5481
                     /locus_tag="ACHBJ1_001496"
     CDS             3007..5481
                     /locus_tag="ACHBJ1_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9430442.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5584..7320)
                     /locus_tag="ACHBJ1_001497"
     CDS             complement(5584..7320)
                     /locus_tag="ACHBJ1_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430443.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7317..8021)
                     /locus_tag="ACHBJ1_001498"
     CDS             complement(7317..8021)
                     /locus_tag="ACHBJ1_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9430444.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8152..8655
                     /locus_tag="ACHBJ1_001499"
     CDS             8152..8655
                     /locus_tag="ACHBJ1_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM9430445.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8615..8953
                     /locus_tag="ACHBJ1_001500"
     CDS             8615..8953
                     /locus_tag="ACHBJ1_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM9430446.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8974..10392
                     /gene="ffh"
                     /locus_tag="ACHBJ1_001501"
     CDS             8974..10392
                     /gene="ffh"
                     /locus_tag="ACHBJ1_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM9430447.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10504..11082
                     /locus_tag="ACHBJ1_001502"
     CDS             10504..11082
                     /locus_tag="ACHBJ1_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM9430448.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11086..11607
                     /locus_tag="ACHBJ1_001503"
     CDS             11086..11607
                     /locus_tag="ACHBJ1_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM9430449.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11686..12240)
                     /locus_tag="ACHBJ1_001504"
     CDS             complement(11686..12240)
                     /locus_tag="ACHBJ1_001504"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM9430450.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12493..12768
                     /gene="rpsP"
                     /locus_tag="ACHBJ1_001505"
     CDS             12493..12768
                     /gene="rpsP"
                     /locus_tag="ACHBJ1_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM9430451.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12780..13031
                     /locus_tag="ACHBJ1_001506"
     CDS             12780..13031
                     /locus_tag="ACHBJ1_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM9430452.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13155..13946
                     /locus_tag="ACHBJ1_001507"
     CDS             13155..13946
                     /locus_tag="ACHBJ1_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430453.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14116..14637
                     /gene="rimM"
                     /locus_tag="ACHBJ1_001508"
     CDS             14116..14637
                     /gene="rimM"
                     /locus_tag="ACHBJ1_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM9430454.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14627..15388
                     /gene="trmD"
                     /locus_tag="ACHBJ1_001509"
     CDS             14627..15388
                     /gene="trmD"
                     /locus_tag="ACHBJ1_001509"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM9430455.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15524..15871
                     /gene="rplS"
                     /locus_tag="ACHBJ1_001510"
     CDS             15524..15871
                     /gene="rplS"
                     /locus_tag="ACHBJ1_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM9430456.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16181..16423
                     /locus_tag="ACHBJ1_001511"
     CDS             16181..16423
                     /locus_tag="ACHBJ1_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM9430457.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16511..16657)
                     /locus_tag="ACHBJ1_001512"
                     /pseudo
     CDS             complement(16511..16657)
                     /locus_tag="ACHBJ1_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16714..17028
                     /locus_tag="ACHBJ1_001513"
                     /pseudo
     CDS             16714..17028
                     /locus_tag="ACHBJ1_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            17057..18316
                     /locus_tag="ACHBJ1_001514"
     CDS             17057..18316
                     /locus_tag="ACHBJ1_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430458.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18369..19265
                     /locus_tag="ACHBJ1_001515"
     CDS             18369..19265
                     /locus_tag="ACHBJ1_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430459.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19276..20145
                     /locus_tag="ACHBJ1_001516"
     CDS             19276..20145
                     /locus_tag="ACHBJ1_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430460.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20155..20830
                     /locus_tag="ACHBJ1_001517"
                     /pseudo
     CDS             20155..20830
                     /locus_tag="ACHBJ1_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21036..22700
                     /gene="alsS"
                     /locus_tag="ACHBJ1_001518"
     CDS             21036..22700
                     /gene="alsS"
                     /locus_tag="ACHBJ1_001518"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM9430461.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22713..23423
                     /gene="budA"
                     /locus_tag="ACHBJ1_001519"
     CDS             22713..23423
                     /gene="budA"
                     /locus_tag="ACHBJ1_001519"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM9430462.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23553..>24005
                     /locus_tag="ACHBJ1_001520"
     CDS             23553..>24005
                     /locus_tag="ACHBJ1_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM9430463.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLL"
BASE COUNT         7939 a         4112 c         4975 g         6979 t
ORIGIN      
        1 taactatgca ctgtaacgat aatgctgcta aaatatcttt ttacgaaaaa catcacaagg
       61 aaaaacttaa ctatggtatt atctaaacat aattttttcg gtatttaata tttaattagg
      121 aggtaatatt ttgctttttt atagtaattt tatcttaata gtagcgatat tattacttct
      181 gaatatatgg atttttgata ggagtagaaa tgcaagtata ggttttagaa ctaaacgaag
      241 tctgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag gaattgtttt
      301 aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt ccacctcaaa
      361 ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt ttttagtgtt
      421 tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt gatataagaa
      481 aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg gtttgttttt
      541 atggatgccg agctttttta taagataagg ctaccgccca taacgaccca aggatttgtg
      601 gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa ggatgataga
      661 agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta gatataatat
      721 tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca gcaaaaagag
      781 ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca agcagtaagg
      841 cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag caagttttta
      901 tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg taaattgaat
      961 cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac tctcatgcca
     1021 ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt gtatagtatg
     1081 atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa tcgatcggct
     1141 ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg taaatgctgt
     1201 tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggattagtgg tttctaggaa
     1261 ttttccagct ttgactgttt tctatttagt gataagtgta ggtctcttat taccaaaatc
     1321 tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag tatttacatc
     1381 tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg agagtggatt
     1441 gaaggattca gcttattcct ccatgtattt cttgacgatc aaaccatggt tctatttagt
     1501 aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca ggataaaaag
     1561 tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta aaaaagcata
     1621 tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta aagaatgcga
     1681 aaaacgttta catgattttt acataactta actatatttt tacagcaacc atacgaggga
     1741 tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa atgaaaaaag
     1801 tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca ggatcaggaa
     1861 atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa gtaaaaatcg
     1921 ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa agctttgtac
     1981 aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact ggtttgagcc
     2041 aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa gaaagagacg
     2101 gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg gcaccaatcg
     2161 tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat atctttacag
     2221 gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac gtcgtgaacc
     2281 gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat ggtgctaccc
     2341 ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta agtcaaacac
     2401 caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa gcactaagta
     2461 tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga atctggtcat
     2521 atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc ttagactaca
     2581 tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta ccaatcacaa
     2641 tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat aaacatgcca
     2701 cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc ccacatatac atttttcctt
     2761 taattgtgaa aaatagttcc tcatattttg ccgttttttg agctaatacc gagggtcagc
     2821 tgattgaaca ccgtttattc gaaaaaaaga gctacgacat agaactgtcg tagctttttt
     2881 tttgctatat gacaggaatg aaaggtgttt gcaagcttaa tgtgaggaac actttccata
     2941 tctgttctat actggatagg agaagtttgg aaatatccag aaaacgaaat caatcgaggt
     3001 agccacatga agaataaatg gataaatcga agtaaaaaaa agaaagtaac acaaagatca
     3061 tcagataaag tcatccgtct ttttccatcg tataaagaag gattaacggc agatcaagtc
     3121 aaggaacgaa tagaaaaagg tgcagcaaat aattcagtcg atcccacatt caaaacaaat
     3181 cagcaaatcg tattggaaaa catatttacg tatttcaatt tgatctttct tatcttagcc
     3241 attttgcttt gtttagtaga atcctataag aacttgactt ttttaccagt tatcatagcc
     3301 aatacaggaa tcgccatttt tcaggaaatt cgatccaaaa agatcttgga cgaactaaat
     3361 gttttaaata cagcaaaagt caatgtcata cgagacggga tagaacaaac aatagatata
     3421 gaagaccttg taatagatga cattgttatt ttagagactg gacatcaaat tccagcagat
     3481 gcagaggtcg tggaagggaa actgcaagtt aatgaggctt tattgacagg cgaagcagat
     3541 gaaataacca aagaaatagg cgatccatta atgtccggca gtttcattgt ttccggaaaa
     3601 gcttacgccc gattagacaa agttggagca gattcgtata tttcccagct gacagtcaaa
     3661 gccaagacaa tgggagaagg agaacagtcg gagatgattg cttctcttaa tcaattgatc
     3721 aaatggattg ggatcatcat tattcctatc ggtatcctgc ttttttctca aagttacttt
     3781 tttaatggaa acacgatcaa agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg
     3841 atacctgaag gactgtactt gctgacgacg attgcattag cattaagtgc gactaaacta
     3901 gcaaagcaaa gagttctttt acacaatatg aaaagcattg agacattggc aagggtaaat
     3961 gtcctttgtg tagataaaac aggaacgatc acagaaaata aaatgtctgt tcaaaaagtg
     4021 atcgtctcta aaaaacaaga aataatcagt cagtcagatc aattggaagc atgcattgct
     4081 gactatgcaa aagcgatggc aggagacaat gcaacaatgg aagctgttaa agcatatttt
     4141 acaaaaacca ctgatcaatc ctatcaccat gttattcctt tttcttctgt ccaaaaattc
     4201 agcagtgtag cgttggcaga caaggtgtat gttttaggag cgcccgaaat ggttttgcgc
     4261 gatcaattgc cggaatatgc ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg
     4321 ttggtctttg gtgtgtacga tgggattctt gaagaaccag tattgacaga agcagtcaca
     4381 ccattaggct atattctgat tgctaatcct atcagaaaag aagcaagagc cacatttgat
     4441 tacttcaaaa aacagcaagt agccatcaaa gtgatttctg gagataatcc gctcaccgta
     4501 tccaatgtag ctgtccaagc agggatttca catgctgatc agtacgttga tgcatctaaa
     4561 ttatcagaag aagaatatga agcagctgtc gaaaaatata cagtttttgg acgcgtcaag
     4621 ccagaacaaa agaaaatctt cgttcaattg ctaaagcgtc gtaacaatac agtagctatg
     4681 acaggtgatg gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg
     4741 gcttccggaa atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt
     4801 tcacgcatgc cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca
     4861 gccagcttgt ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc
     4921 tttgctctga cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc
     4981 ggacttccat ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc
     5041 atcatgaatg tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt
     5101 gcgttggtga tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct
     5161 accatgcttt tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat
     5221 aaattcagaa gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt
     5281 ttgcataagc tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt
     5341 ctatttttct cggcagattc gatttttagg catttgacca cattggtaga atatttgttt
     5401 gaacgtaaaa atgaaaaaga gcaaaagccg tttagtcgcg tagttaaaaa atttctatcg
     5461 atatttaaac gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac
     5521 tcgtgtatct ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct
     5581 tttttacatt ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac
     5641 agtaatcgta ccatgaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt
     5701 cccgcctgaa tgccggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc
     5761 ctgatcctca atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt
     5821 tgcctgatag cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat
     5881 cagatttttg ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc
     5941 aatcaatcga atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg
     6001 ctggaaataa ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga
     6061 cagctggatg atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc
     6121 aagaaaagaa gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa
     6181 accaatcagt gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt
     6241 ttgtagcttt tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc
     6301 ggtaccttct gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt
     6361 aagggtgatc tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat
     6421 gagttttggc tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt
     6481 taaatgtagc tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag
     6541 gaatacgcca atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtata
     6601 cgcccgatac atcttactca tttgttcgct caacgcattg atcgtctgat acaattcttc
     6661 ccattgatcg ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt
     6721 tagtacgggc agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat
     6781 tcctgcaaga acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc
     6841 agttctcggt aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc
     6901 actttttttg actggtaatg cgacatataa aagatcccca ttcaaagtag tacttttgcg
     6961 caaagcgcta cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg
     7021 ctgttcattc aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa
     7081 agtaatccgt tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc
     7141 ttggaggttt tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct
     7201 ttcttgctgt tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa
     7261 aaacaaacta actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat
     7321 cgtttgggtt cctgaaactt atagccaaat cctctaatag tcaccaagta ctttgggtgt
     7381 tttggatctc gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt
     7441 tgtcctgcga agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg
     7501 tcttttcgtt tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga
     7561 tggtcgtcta cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga
     7621 tattcaggct cagcttcatc ttttgattgc ttacgaggtt caattcgtct gaatattgct
     7681 ttcatacggg ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct
     7741 atttctaggc caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt
     7801 gtttcatttt tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc
     7861 atcacatcta aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct
     7921 ccatctgtgg cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt
     7981 acaatagaag gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag
     8041 gtttcattct gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa
     8101 taaatggtat aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga
     8161 atcagtgcat gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca
     8221 gaattgatgg aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc
     8281 ggattaccaa ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg
     8341 aaagatcaag caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat
     8401 ggcgcgatca aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag
     8461 gaaaaaagcc cttcatgcgg aacccattcg atttatgacg ggagtttttc cggaataaaa
     8521 aaaaatggtg ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct
     8581 gatgaagaat ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat
     8641 gaacgcgctc tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga
     8701 attatattat gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg
     8761 acaagcagta tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa
     8821 acttcatctt ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata
     8881 tattacagat aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc
     8941 aatcgaagaa taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt
     9001 ctccagcagg cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa
     9061 gaaatgatgc gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc
     9121 aaagatttta ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta
     9181 tcacctgcac aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct
     9241 gaaacagcag agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt
     9301 caaggggcag gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa
     9361 aatgctcgtc cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg
     9421 aaagtattag gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg
     9481 gtagagatcg tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg
     9541 atcgatacgg caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc
     9601 aaagaattga cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat
     9661 gccgtcaacg tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact
     9721 aaacttgatg gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca
     9781 ccaatcaaat tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat
     9841 cgtatggcta gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag
     9901 caagactatg acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc
     9961 gatttcaatg actttattga acagcttgat caagtgatgg gtatgggccc actggaagat
    10021 ctaatcaaaa tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat
    10081 ccaaaagatg ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca
    10141 aatcctgatc tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt
    10201 gttgttgaag tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa
    10261 atgtccaaag gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa
    10321 ggaaaactag gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa
    10381 aaacggaaat aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag
    10441 cttttctatc agcaattttt attctttact aatacaatta ctctaaacga agaggtggca
    10501 cagatgagat gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc
    10561 tggggtgttc ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat
    10621 caagccggat tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat
    10681 gatcgattcg atattaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg
    10741 gaaaacaaag gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa
    10801 gcagtcttga aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct
    10861 aatggaaaaa tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct
    10921 ctttcagatg aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca
    10981 acaatctatg cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc
    11041 cgcaaagaag aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata
    11101 ttttatggtg ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg
    11161 ctttttgaca ctccaacagg taagcaaacc acttatgaag ctttttatga aacaatcgat
    11221 acgaccatca tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca
    11281 atttatccag agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat
    11341 gctgaggtcg taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga
    11401 aaagatatct gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg
    11461 attgatgaat ggtatatcca gattacccct gtattattag gggacgggat tcctttattc
    11521 caaaaaggaa catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt
    11581 atcgaattgc attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca
    11641 gaaatgtcct gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt
    11701 gttagaaaaa tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac
    11761 gatattacca ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg
    11821 caaggttggc acattcaaaa agacaagtga ttgtcgtaga tggtggtttg cgccaaagcc
    11881 gccgatccca cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg
    11941 tccataaggt cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt
    12001 gtattcagga gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt
    12061 ccattcagca ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc
    12121 gatttttaca aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac
    12181 ttttttgttg taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat
    12241 acgatttttc ctccttcaaa ctatctataa caatcttaca aaaaataagt aactggagca
    12301 aataaaaaca cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa
    12361 agaagtctaa aatcttctat ttcctaaaaa aaatcgaatt taatcaagct gtaaagaaaa
    12421 aaagctttac agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg
    12481 tggaaataaa taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg
    12541 ttttaccgta tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact
    12601 gtaggtactt acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt
    12661 cttgactggt tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa
    12721 gaaggcgtta tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata
    12781 tgaaagattt aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc
    12841 aagtcaagtt agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc
    12901 ctgaagatat tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa
    12961 tcgtttacgg tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata
    13021 aggaaaagta aataaacgtg ctgtactcga attttcgagt gcagcttttt tgttttttta
    13081 ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag
    13141 ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg
    13201 cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa
    13261 ctgttaccag tccagaaaaa agtagtacca aaacggctac caccactgaa caaacaatgg
    13321 ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg
    13381 ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata
    13441 caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg
    13501 acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta
    13561 gtcaagaaac aatccaagtt ttgcagccct ttgagatcga cacaaatggt cagcaagtgg
    13621 acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat
    13681 ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct
    13741 tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac
    13801 aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct
    13861 ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga
    13921 tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact
    13981 ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat
    14041 accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag
    14101 aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt
    14161 atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa
    14221 ggaacggtgt taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt
    14281 catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat
    14341 gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct
    14401 gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag
    14461 ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt
    14521 ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta
    14581 gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt
    14641 attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc
    14701 tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa
    14761 acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca
    14821 gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt
    14881 aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca
    14941 agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac
    15001 attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc
    15061 gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc
    15121 agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc
    15181 agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga
    15241 agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga
    15301 aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca
    15361 agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tattctttac agtaggttga
    15421 taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctat
    15481 gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga
    15541 agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt
    15601 gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt
    15661 tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa
    15721 cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt
    15781 agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa
    15841 agctgctcgt atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct
    15901 tggtattcaa gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttt
    15961 ctttaattat tggtagggga gaataatgga ttagaataaa tctacctata agtagtagta
    16021 aaaaaaagag cactatgata atatctttct taagagtaac cgctttaata aaaaatccag
    16081 aatattctaa cataaatgga gtattcttag aatcaaaagg agaaagaaga tggtttatac
    16141 ttatacggag caagaaaaaa tgaaagtttg agacacattc atgaatgatg gaaaagtaaa
    16201 acaagtacca agttcaacaa aaaagaaaaa catcctattg aaagaagtct tgaaacgctt
    16261 tgaccatgga gtgacttata cagaaacaga ggttaattcg atactgctga acgtattttc
    16321 tagtggcgat tatgtcgaac aaagacgtta tctaatcaca tttggctttt tcaaaagaag
    16381 ttcagacggt cgggcatatc agatgatggg aattgaaaat tagaagtttt aatttatact
    16441 cattgaaaaa agttcaagta tgcgatattg gttctacacc gcatacttga actttttttg
    16501 tcatctgtat ctataataca aagccaagaa cgttattcaa aatatggata gctatgataa
    16561 aagctaatga gcgattcgca gttaaataag caatcgtcag taaaatcgtt ccaggagcgt
    16621 acatgaaaaa atcaattgga tgcgttacat gaataaagat aattctcttt tatttgtacg
    16681 aataaatacc aaaacgtacc tcctaaaatt agattcaaag aagatagcaa agaggaaaaa
    16741 gatagttctg cagcagctat agcggcatgt ggattgctag aactttccaa acaactgcct
    16801 gtttcagatc ctcgtcattt cgattacgaa gagatagcaa tcaaaatctt ggaagaatta
    16861 cagaaaaact atacaacgaa acgagttaac ggatcaaacg gtctgcttct ccatgccgtt
    16921 tatgataaaa acagcttaaa aggtgtagat gaatgtgtga tctggggcga ttacttttat
    16981 gtagaaggaa tcactcgtct agctaaaaca tggtactgtt actggtgatg tataagagaa
    17041 gaggaggatt catataatga agcgatggaa aaaaatgatg attggatcaa tagttttagg
    17101 aatgtcactc gtatttggtg cttgtggtaa tcaagaagac agtagtgaag aatcagcaaa
    17161 tgaaattacc gtttcgacat ggaattatga aacaacacct gaattcaaag cgctctttga
    17221 agcattcgaa aagaaaacgg gaatcaaagt gaaagcagta gatattgctt cagatgatta
    17281 tgatacgaaa ctcaccacga tgctttcttc tggagacacg acagatgtat tgacgatgaa
    17341 aaatcttttg tcgtattcaa attatgcttt acgagatcag ttagtcgatc aaacagaacg
    17401 aattagagaa ttagacactg atgctgctga agaaacatat gaaatgtatg atatcgatgg
    17461 aaaaacatat gcattgccat atcgaacgga cttttgggta ctatattaca ataagaaaat
    17521 gtttgatgat gcaggaatcg aatatccaaa aaatctaact tgggacgaat atgaagattt
    17581 agcaaaaaaa cttagtaaaa atgacggaca agtttacgga gcatatcaac atatctggcg
    17641 ttcgacaatc caagctattg cagctgctca aaacgaggcg aatcttgttg aaccagatta
    17701 tcaatatatg actgattatt atgatcgagc attgaggatg caaaaagaag gcgcgcagat
    17761 ggattttggc acggctaaat caacgaaagt aacctatcag tctcaatttg aagaacaaaa
    17821 agcagcaatg atgtacatgg gaacttggta tatggctggc atactcgcaa acaaagaagc
    17881 aagtaagaca gaagtagaat ggggaattac agctattcct cagaagaaaa aaggagaatc
    17941 ggtcacgacg tttggctcac caacagcatt tgctgtcaat aaaaactcaa agaaacaaaa
    18001 agcggcgcaa gaatttattg aatttgcagc cagtgaagaa ggagcaaaag tattagcagg
    18061 agtaggagta gttccttcat atcgtacgga tgagattgat cagctttatt ttaatctcgc
    18121 tggaatgcca acggatgaaa tttctaaaaa agctttttct cctgatgaaa tcaaacttga
    18181 atttccgatt gatacgtatg ggcctgcaat cgataagatt ttacaagaag aatatgactt
    18241 agttttggtt ggagatgaaa cgccggaaaa aggaacggta aatatggaga aacgtgtagc
    18301 tgcggaaaaa gaataatcat gaaaggagag aagccaagag ataaaacact cttggtttcg
    18361 tcatcgaaat ggagaatagt ataaataaga aagctacaaa tcatttgcgt agaaaacgaa
    18421 cgtggatcgc ttattcgttt atcgccccga atttcattgg ttttttcgtt tttacattaa
    18481 taccaatcct gttttcgctt gtactagcat ttatgaaatg ggattctttt tcaacgccag
    18541 aatttgctgg actagataac tttcgtaaaa tggccaacga tgagacattt tggatttctt
    18601 tgaaaaatac ttttttatat accgctgctg tagtgcctgg cacacttgtt tgttcgttag
    18661 ggctagcttt actactaaat cagaaaatta gagggatgaa gctttttcgt acgatatttt
    18721 tctttccgta tgttacttcc ttagtagcaa ttgctgttgt ttggaatatg ttgtttcatc
    18781 caacagtggg acctattaat atctttttaa gacgatttat tgaacatcct ccaggttgga
    18841 cttcaagttc aaattgggcg ctttgggcaa ttgtaattgt cagtatttgg cgagggatgg
    18901 gttattacat gattctatac cttgcaggac tacaaagtat ccaaaaagaa ctatatgaag
    18961 cgtcttcgct agatggagca aaccgttgga aacaattttt gaatgtgaca ttaccttcgt
    19021 tgagaccaac aaccttcttt gtaacgatca tgcttgtcat caattgtttc aaggtgtttg
    19081 accttgtgca agtgatgacg gatggcggcc ctggtcgagc gacaaatgtg ctggtttacc
    19141 aaatctacaa tgaagcattc aaaaaatttg attttggtta tgcttcagct atttcacttg
    19201 ttttgttcgt aattgttttg ggcgttacgg ttgttcaatt ttcttggaat aagcgtcgta
    19261 cttaggaggt gtgatatgga agagagtgtt cgaaatgaaa gaaaaaaagc aaaaattcgt
    19321 tcaaaaaaca tgccctttat tcaatggttg ggtatgattc tgctgattgt attagcttta
    19381 ttgacgatca ttcctttttt atggatggtt tctgcttcat tcaaagcaaa taatgaagtg
    19441 ttaacgattc cgattcaatg gatccctaaa acttggcatt tagagaatta tctggttatt
    19501 tggtctcgta ttcctttagt gacatttttt aagaatacag cttttttaag cgttgtgatc
    19561 acgttgattc agttgtttac ttctagtttt gctgtttacg ggttttctga actaaatttt
    19621 aaagggcgcg atattttatt tttagcatat atcggcacga ttgctgtccc ttggcagtct
    19681 tatatgatcc cgcagttcat tatgatgaga aaagtgggtt taacagatac gttgtggtcg
    19741 ttggtgctat tacaagcgtt caatgctttt ggtgtgttct tgttgaagca atattacagt
    19801 agtatcccag attctttatg tgagagtgcg cggatcgatg gcttatcaga gtgggaaatc
    19861 tatcgcaaga tcattttacc tcttacaaaa ccggcgattg ccagtttgac gatcatcaca
    19921 ttcgttaata catggaacga ttatatgggg ccattcattt atttgtcttc tacggaaaat
    19981 aaaacaatcc agttagggtt gaaaatgttt attgggctat ttgatgcaga gtacgcatta
    20041 atcatggctg cctcagttgt ttctgttctt cctgttgctt ttatattttt agcgatgcaa
    20101 aaatattttg tagaagggat cgcttcatca ggaatgaagg aataggaggt gattatgaaa
    20161 gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt attggttgta tcaatcattg
    20221 gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata tagcagctgc gttccttttg
    20281 tcgatagatg gaaggaatgt cttttccttt attttttctt tactttttat tggtccaggt
    20341 tgcatcgtga tgataggtat gacagaagaa ttagcaaaag aaaaaagttt ttcaccagta
    20401 cgtttgttca taaagacgtt tcgccaattt tggctaaagg gattttttta ttggctattt
    20461 gcttggatag ttagtgtgat aatgattttt gattgtttct tttttatacg tttttcttat
    20521 ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt ttacttgctt gtttatcagt
    20581 ttcctttagc atcaactgct ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    20641 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    20701 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    20761 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    20821 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt ttatgtaaaa
    20881 actgtcattc tttttcattt tttgaaacat atggctaaac aaatgaaata aaacatgcta
    20941 tacttttgaa tgaaacaggt ttagaaactg tatcacttat ttgtgattat ttctaactaa
    21001 taattaaaaa taaagcataa aaaggacgat gttcaatggc tgaaaaaatg aaacaaggtt
    21061 cgaaatctgt agtagaaagt ttgattaatc atcatgttga ttatgtattc ggtataccag
    21121 gtgcaaaaat cgatggtgta tttaatgaat tagaggatca aggtcctgaa ttaatcgtga
    21181 ctcgtcacga acaaaatgct gcatttatgg cacaagcaat cggacgtatt acaggagagc
    21241 caggagtagt tatcgcaaca agtggtcctg gtgctagtaa cttagctaca ggtttggtaa
    21301 cagcgacagc agaaggtgac ccagttttag caattgccgg acaagtaaaa cgaagcgatc
    21361 tattgaagtt gacccatcaa agcatggata atgcagcact gttccagcca atcacaaaat
    21421 acagtgcaga aatccaagat ccagaaacaa tttcagaagt gattgccaat gcttatcgta
    21481 tggctaagag ttcaaaaaaa ggcgcgagct ttatcagtat cccgcaagat gtagtggatg
    21541 cacctgtcca aggtaatgtc atcaagccat tgagcgatcc taaattaggt tctgcttctg
    21601 cagatgatat ccgttaccta gctgaacgca ttagagaagc aaaactaccg gtactattag
    21661 taggtatgcg aggatcaagt gaaaaagaaa cgttagccat caggcaatta gttgaaaaga
    21721 cagcattgcc tgttgtagag acattccaag cagctggagt gatctcacgt gaattagaag
    21781 cgcatttctt tggtcgtgtg ggtttattta gaaaccaacc aggagatatg cttttgaaac
    21841 gaagtgatct agttattgca attggctatg atccaatcga atatgaagca agaaactgga
    21901 atgcggaaaa agatgcgcgg atcattgtga tcgatgaagc gccagctgaa atcgatcctt
    21961 ttatgcagcc tgaaagagaa ttgatcggtg atatctcagc cactcttgat ttattgacag
    22021 gttcattaga gcctcaacaa gtgtcagaag atgcaaaaga atatttagca tctcttcaag
    22081 caaaattaac ggagcgggac attgttcaat caaaaggaga agcaggtatc ttgcatcctt
    22141 tagaagttat taacacgctt cagtcaaaag taacagatga tatgactgtt accgtagatg
    22201 taggtagtca ttatatttgg atggcacgcc actttagaag ttatgaacct cgtcatttgt
    22261 tattcagtaa tggtatgcaa acgcttggtg ttgcattgcc atgggcgatt tctgctgcat
    22321 tggtgcgtcc aaatacacag atcgtatcag tttccggcga tgggggattt ttattctctg
    22381 cccaggattt agaaacggcg gtacgtaaaa aattgaatat cgttcatttg atttggaatg
    22441 atggacacta caatatggta gagttccaag aaaaaatgaa atatcagcgg gcgtcaggtg
    22501 tagactttgg tccagttgat tttgtgaaat atgcagaagc ttttggcgct aaaggaatcc
    22561 gagctacaag tgtagaggaa ttagaaaaag ctttagaaga aggatttgcg acagaaggac
    22621 ccgtaattat tgatattccg attgattacc gtgataatga gaaattggga gaaacaatct
    22681 taccagatca attctattaa ggaagtgtcg acatgactga aaaaatctta taccagcacg
    22741 ggacattagg agctttgatg gccggcttga tggatggaac agaaacgatt gctcatatat
    22801 tagaaaaagg aactctggga ttgggaacat tgcatgggct tgatggtgaa gtcatctttt
    22861 tagatggcgt agcttatcaa ggaagatcag atggctcagt tgcccaatta gatggaagtg
    22921 aacttacccc ctatgcagca attacagatt ttacgccaga tactagtttt tctgtctcaa
    22981 aaactgcaga tggagaacaa ttgaaaaaag acatattagt aagagaagcc ggggaaaacc
    23041 tatttttggc agtgaagata actggattat tcaaaaatat gcacatccgt atcatgccga
    23101 agcaagaaaa accttacaga agactagcaa aaatttcaga gtctcaacca gaattccaac
    23161 aaagcaatgt ccaaggaaca ttagtcgggt tctttacgcc tgaattattc caaggagttg
    23221 ctgcagcggg atttcacctg cattttatcg atgatacaca tacatttggc ggacatgtaa
    23281 tggattttga agtggctgaa ggaaaagttg agattagccg catctcgtcc cttgtacagc
    23341 attttccagt tgatgatccg acttttgttc acgaaacgat tgattatgct gatttagcag
    23401 cagaaatcca acaagcagaa tagaaaaaga cagcgagatg cgttcaaggt gattaacctg
    23461 acgtatctcg tttttttgag gagaaaacct agcgtttaca aaaaaactga gctataatct
    23521 tctccataga tcaaaaagga gcggttttta tcatgtggac agaaaaacag aaaaaagatg
    23581 catacgaaat tttattatta cagcaacaag gattattaga agcagaagat aactggttag
    23641 caaatttagc aaattcatct gcattgttga atgagacttt gcctgagact gtctttgcag
    23701 gatattattt attcgatggt aatgaattga ttttaggacc gttccaaggg cgagtttctt
    23761 gcacacgtat caagatggga aaaggagttt gtggagaatc agctgaaaaa caagaaacat
    23821 tgatcgttga tgatgtcaaa acccatgcaa attacatttc ctgcgattca gccgcacaat
    23881 cagaaatcgt agtgccaatg gtaaaagaag gtaatttaat cggtgtttta gatatcgatt
    23941 gcggagtaac gaaagggtat gatgaaatcg atcaagcatt tctagaaaaa tatgtagatc
    24001 tttta
//
LOCUS       DAYYNQ010000043        22978 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000043 DAYYNQ010000000
VERSION     DAYYNQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.043"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..1272)
                     /gene="queG"
                     /locus_tag="ACHBJ1_001521"
     CDS             complement(121..1272)
                     /gene="queG"
                     /locus_tag="ACHBJ1_001521"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM9430464.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1291..2043)
                     /locus_tag="ACHBJ1_001522"
     CDS             complement(1291..2043)
                     /locus_tag="ACHBJ1_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430465.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(2184..3133)
                     /locus_tag="ACHBJ1_001523"
                     /pseudo
     CDS             complement(2184..3133)
                     /locus_tag="ACHBJ1_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(3134..3973)
                     /locus_tag="ACHBJ1_001524"
     CDS             complement(3134..3973)
                     /locus_tag="ACHBJ1_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM9430466.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(3970..4716)
                     /locus_tag="ACHBJ1_001525"
     CDS             complement(3970..4716)
                     /locus_tag="ACHBJ1_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM9430467.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            4954..6324
                     /locus_tag="ACHBJ1_001526"
     CDS             4954..6324
                     /locus_tag="ACHBJ1_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9430468.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6379..7275)
                     /locus_tag="ACHBJ1_001527"
     CDS             complement(6379..7275)
                     /locus_tag="ACHBJ1_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9430469.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7389..7559)
                     /locus_tag="ACHBJ1_001528"
     CDS             complement(7389..7559)
                     /locus_tag="ACHBJ1_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430470.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7556..8233)
                     /locus_tag="ACHBJ1_001529"
     CDS             complement(7556..8233)
                     /locus_tag="ACHBJ1_001529"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM9430471.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8324..9229)
                     /locus_tag="ACHBJ1_001530"
     CDS             complement(8324..9229)
                     /locus_tag="ACHBJ1_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM9430472.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9404..10828
                     /locus_tag="ACHBJ1_001531"
     CDS             9404..10828
                     /locus_tag="ACHBJ1_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM9430473.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            10874..11905
                     /locus_tag="ACHBJ1_001532"
     CDS             10874..11905
                     /locus_tag="ACHBJ1_001532"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9430474.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11912..13099
                     /locus_tag="ACHBJ1_001533"
     CDS             11912..13099
                     /locus_tag="ACHBJ1_001533"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM9430475.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13255..14637
                     /locus_tag="ACHBJ1_001534"
     CDS             13255..14637
                     /locus_tag="ACHBJ1_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9430476.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14609..14767
                     /locus_tag="ACHBJ1_001535"
     CDS             14609..14767
                     /locus_tag="ACHBJ1_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430477.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKMKKKHA
                     NSLFLNNK"
     gene            14764..14994
                     /locus_tag="ACHBJ1_001536"
     CDS             14764..14994
                     /locus_tag="ACHBJ1_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430478.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15194..15862)
                     /locus_tag="ACHBJ1_001537"
     CDS             complement(15194..15862)
                     /locus_tag="ACHBJ1_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM9430479.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(16051..17477)
                     /locus_tag="ACHBJ1_001538"
                     /pseudo
     CDS             complement(16051..17477)
                     /locus_tag="ACHBJ1_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            17720..18175
                     /gene="tnpA"
                     /locus_tag="ACHBJ1_001539"
     CDS             17720..18175
                     /gene="tnpA"
                     /locus_tag="ACHBJ1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM9430480.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            18440..18538
                     /locus_tag="ACHBJ1_001540"
                     /pseudo
     CDS             18440..18538
                     /locus_tag="ACHBJ1_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(18761..18991)
                     /locus_tag="ACHBJ1_001541"
     CDS             complement(18761..18991)
                     /locus_tag="ACHBJ1_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM9430481.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(19221..20039)
                     /locus_tag="ACHBJ1_001542"
     CDS             complement(19221..20039)
                     /locus_tag="ACHBJ1_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9430482.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            20200..20889
                     /locus_tag="ACHBJ1_001543"
     CDS             20200..20889
                     /locus_tag="ACHBJ1_001543"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9430483.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            20903..22405
                     /locus_tag="ACHBJ1_001544"
     CDS             20903..22405
                     /locus_tag="ACHBJ1_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9430484.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            22418..22894
                     /locus_tag="ACHBJ1_001545"
     CDS             22418..22894
                     /locus_tag="ACHBJ1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9430485.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         6846 a         4332 c         4256 g         7544 t
ORIGIN      
        1 aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa aaatacaaaa
       61 aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt aactattttt
      121 ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta taacgacttc
      181 ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg gttcttttgt
      241 tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga catctgttaa
      301 acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag caatcaatgc
      361 gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa attgctgttt
      421 gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatattt cgtctacttt
      481 tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc agactaactg
      541 gcagatatca catccataaa tgacatttcg cattttttta cgatattctt ccggcatcat
      601 cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc cgtcaccaag
      661 caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac caaattctcc
      721 tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga caaatgaacc
      781 gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag ctcgctgtgc
      841 tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc attcctcttc
      901 tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca aacggtctct
      961 gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc gtttttcatc
     1021 cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa tgatcgattt
     1081 cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat gttcaaagcc
     1141 agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat caaaaggttc
     1201 cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta tgatttcttc
     1261 ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg tataacccat aatatagttt
     1321 ttcttttttc agctggacaa actggatctg ctgatcggca atcttctttt cttccatcca
     1381 tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc ttttgttatc
     1441 taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat ggagccttac
     1501 ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt catagagatt
     1561 tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctagcaa tttctactag
     1621 acgattataa aacatattca ttaatttgct gttggcaata aattgacttt tataataggc
     1681 cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg caggccatgc
     1741 tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc cgactggaga
     1801 aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt catcaaacaa
     1861 ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat ttgctatgaa
     1921 taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg tattttcctg
     1981 ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac gtgtaaaatt
     2041 catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa aaaggtatct
     2101 gtacgacgaa aaacagcata attttttttc atatctctta taaaaaatgc aaggtctgct
     2161 cctcaagcta gaccttgcac ttttcagctt tcttttgttt gtgacaaacc ttcatggatc
     2221 gtttcaatat tccatttcat catctgatag taagaatctc cttgttctcc ttcttttgct
     2281 atggaatcag tgaacagttt atcatagatt ttcaaacccg tttcttttgc tacccgttcc
     2341 atgcttcttg agtctacact ggtttccacg aataaaaccg gtacttcttt tgctcttatc
     2401 tgatcaatga tcgctttcat ttgatcaggc gtaccttgac tttctgtatt gatctcccat
     2461 atataagctg ctggcaaatc atatgctcgt gaaaaatact tgaatgcccc ttcacttgtt
     2521 actaataatt ttttgttctc ttcgatagaa gcaaaacttt ccttcgcttg tgtatctaat
     2581 tcttttagtt tttccacata tgcttctgca tttttttata gagtgtcgca ttttctgcat
     2641 ctttttcaga gaatatacgt gcgatttcct ccacatattt tattccgtta gataggtcta
     2701 gccaagcatg gggatctgct tttgtatgtt cttcaccgct agttaaataa agaggttcta
     2761 catttttgct aactgcaaaa taatcttttc cttcttcttt tttagccgtt tccatcaagt
     2821 tatcgaacca gctgttacct gtttcaagat tcaaaccgtt gtataaaata acatctgcat
     2881 cgcttgcctt tttgatgtct tctggtagta cttcatattc atgcggatct gttccgactg
     2941 gtacgatact gtggatatct atttgatctg ttccgacctc ttttgccatg tccgccaaga
     3001 tcgaattggt agccacgact gacaactttt ctttacttcc ttcatccgct tggtcagaag
     3061 cctgctttcc gcaaccggct aataccaaga aactaactgc caagattaaa aagagtgatt
     3121 tttttgtttt catctatctg cttcctttct gaaataaaat tccttttgtt ggagaaaata
     3181 aaaaagcaag gataaaaaat gcggtacatg ctaacacgat cgtagctcct gatggccaat
     3241 tgtaactata actgaaaaac acaccgacaa ctgcacttag cataccgatt cctgcagctg
     3301 taatgatcat tttcagtaaa tggttcgtta gcaagtaagc cgtggcggct ggtgtgatca
     3361 acatggcaat caccaagatt gttcctactg tctgtaaact gactacagcc actaatgtca
     3421 aaaagaacat caaaagataa tggatcagcg atgtgttcag cccataagct tttgccattg
     3481 tcggatcgaa tgatgtgatc ttcaattctt tataaaagaa aaagacaaat atcaagacga
     3541 ttccactaac gagtgctgtc agcaacaaat cgctttccct aacagctaag acattcccaa
     3601 ataaaatatg atacaaatct gtcgcactct gggcatatga aatcaagata atccctaatg
     3661 caaaaaagga gctaaagaca attccaatcg ctgtatcatt tttcaatcgg ctccgctgag
     3721 tcacgaaacc aatcactgct gctgttagca tcccaaaagc agtggctcca aagatgtaac
     3781 taaaaccaaa catataagaa atggctacac ctggaagaac tgcgtgagag atagcgtctc
     3841 ccatcaaaga catccctcgt aaaataataa aagaaccgat gactccagaa gcaagtccta
     3901 caagcatgga agtgaccaaa gcgtattgca ggaattgata attcattaat ccatcaataa
     3961 aggattggat cattttttct ctcctccttt tactaaatgt ctgatgatct caccataggc
     4021 caattgaagg ttcttcgatg taaagacttc ttgtactgga ccggaagcga tcagctgttt
     4081 atttaaaaga ataacctcat cgaaatattc ttctacttta tgaagatcat gatggacgat
     4141 caaaatcgtt tttcctgaat tcttcaattc ctgcaagatg tcaaagatct ttcgttcact
     4201 taacgcatca atccctacga atggttcatc taaaaagatc cattctgctc cttgggctag
     4261 agctcttgca ataaaaactc tctgaagctg tccacccgat agttcgctga tctgtctttt
     4321 ggcatattct tccatcccta cttttttcaa agcttgtctt gcacgttctt tttctttttt
     4381 cccaggtctt tgtccaattc gtaaagatgg atatgttcct aaaagtacaa cgcctaatac
     4441 atcaattgga aaggaaagat ccaattcact tcgctgttcc acgtaggcta tttttttacg
     4501 gactgagttt accgactgtc cagaaaaggt gactttacca gtagtagctg gtatcaactc
     4561 tagcaaagat ttcataaatg ttgattttcc tgcaccgttt ggtccgataa taccggtaat
     4621 tttttgtgga tttatcgtca agttgatagt tttcaaaaca gttttccctt gataagcggc
     4681 tgaaaggtct tgaattacaa tggccgtacg ttccaaatct tacactcctt tttaggttta
     4741 cctaactttt tatttaaata tagtttaact aaaatggaaa gcattttcaa gtagaatgat
     4801 gaaaaagttt ttttcgaatt ttcctcgcat tttatgaaaa ttttctgtag aatcacatca
     4861 agagttcaat aaggctaacc taataatgaa aaacatgtgt tgaaacgttt gattattttt
     4921 tcttgttgac tcaacaacga aaggagtttt tgtatggaag aagaaaagtt aactcgaagt
     4981 cttagtgccc gtcatatcca aatgatcgca ctaggtggaa cgatcggtgt cggtctgttt
     5041 atgggtgcct cctcaaccat acgttggaca gggccctcag tgatgggagc ctatgcgatt
     5101 gcaggattct ttttgtattt gattatgcgt gctttaggcg aaatgcttta cgttgatcct
     5161 tcgactggtt cttttgcaaa ttacgcgagc gagtacatcc atcctgtggc tggctacttg
     5221 accgcttgga gtaatatttt tcaatatatt gtcgtaggaa taagtgaagt gattgctgta
     5281 ggatcttata tgaattattg gtggcctgat ttgccggcaa ttattcccgg aatcattgtc
     5341 gtcttgtttc ttatgctggc aaatcttatt tctgtcaagg cgtttggcga attagaattt
     5401 tggttttcga tcatcaaagt cataacgatc attttgatga tcattgcagg tcttggggta
     5461 atcttatttg gatttggtaa tcatggtcag gcagtaggga tttcaaatct ttggaaaaat
     5521 ggtggattct tcactggagg cgtcaaagga tttttctttg cattatcgat cgttgttgct
     5581 tcctatcaag gaatcgaact gatcggaatg actgctggtg aagcagagaa tcctaaaaag
     5641 acgatcattg aagctgtcca gtccactatc ggtcgaatct tgatttttta tattggtgct
     5701 atttttatca ttgtgagtat ctatccgtgg aatcaattga gtgagattgg ttcacctttt
     5761 gtacaaactt tttctaaagt cggcattaca tttgctgcag gactgattaa ttttgtcgtg
     5821 attacagcgg cattatcagg ttgcaattca gggattttca gcgcaagtcg tatggtctat
     5881 acacttgcca taaatggaaa gatgtctaaa aaatttctga aactatctcg tcacggtgcg
     5941 cctttttatc ctgtcgtagc aatctcaggt ggaatcttaa ttggtctgat cttaaattat
     6001 cttctaccaa tgttcatcaa ccagtcagaa aatattttcg ttttcgtata cagctcaagt
     6061 attttgcctg gtatggttcc ttgggtagtg attttgatca gcgagattcg cttcaaaaag
     6121 aagcacgcag ataaaatgca tgatcatcct tttaaaatgc cactatctcc ttatacgaat
     6181 tatgtgacac ttgctttttt agctatcgta ttgattttca tgtttatcaa tcctgaaacg
     6241 agaatctcat tgattattgg actcgttttc ttgctttaca tgacgatcca ttactttgta
     6301 cgcgaaaaaa gaaccggaaa ataaaaaaag aacgaagctt caccttagtg gaagcttcgt
     6361 tcttttttat tgcttttatc aattcaattt tagcgtctca ccgggagtca ggaaataatt
     6421 ctccatgctc atcccattca gcgacaagat cgtttctact ggtacgccag ttcgttcggc
     6481 tatttgattt ggaccttctc cttccaaaac tacggttgtc gcagcctgtg aagtcgcgct
     6541 gttggtatta gtttcagagg aatctgttgt gtcttgtgtt gttcgagctt gcatttcaga
     6601 agcttctacc gactctgctg tctcagaatc agaagatgtt gtgtcttgtg tgcttgttga
     6661 ctctgcttga agagaggcgc tagtctgcaa ataaatatcg gaaatcttag cgacatgatt
     6721 cgtataatta gccgataatt gattgatcaa agtatctaag tctacaccgt ctttattttc
     6781 tgctgcctga tacgctgcac gtaattttct gatcccttcg tcggagattg cttgaagctc
     6841 acgtgctttt tggttagcaa ctgctgataa accagctatt ccctcttgat ttccctgaat
     6901 ttctgtctgg tattcttcaa tcaatgcagg cgtcttttct tcgatctttt tcgaataatc
     6961 atttacgatt gcttgaagtt gctctggcaa accatttgct gttttttctt cagaagtttg
     7021 tgtctctttt gttttagttg atgactcact tgtttgtttc gtctctgttt tctttgtcgt
     7081 tgtttccgtt atggtcgtac ttttcgcttc tgacgaattt tttccatgaa aatatagacc
     7141 cgcaactcct cctgctatga tcaaaaacac ggcaactgct gccccaatca tgatcatccg
     7201 cttacgagct gcctgttttt gctgtcttct tcgggtcatc cgaacatttt ctccccactc
     7261 attttcatca tacatttttt tcaccaccaa tttttatgta taatagcgtt atcaaacgct
     7321 tcatattctt attgtagcaa ataattgtcg ctgtgcacat ttttatcttt gttatttata
     7381 agaaaatttc accagttgag atgattccaa gattgtccgt cgatttctcc ttgcagctgc
     7441 attgttcgtt cgtctatctc agccatcagt ttctttattt catataagag cgcacggtct
     7501 tgatattctg atttttcgct taattcatct aattgttgga tcagccaatc atttgtcata
     7561 atgaagttcc tttcatttct tgcagtgtaa aggtcaagtc ttcgatctgc tcttttgcct
     7621 cagtcagtgc ttctacgata tatgcttgtt tttcagattc ttcttcctgc attttctgat
     7681 tttcctgtac tctttgaata aaccagtcaa tccgctgagt cagcgatggt ttgccttcca
     7741 atgagagata ctgctgataa gcaattagct tctcccaacg tttttcctgg gataaataca
     7801 taaattgtgc taaaacgaat ggcggaagat aggtcatatg gaatttattt gcgattcgct
     7861 gatacggcct caaaaattcg gaaatcgtat attcttcttt tcctccagct tggtattctt
     7921 tttccgccac acctgttgtc aatacgattc ctagttcttt cccttttaat acatgtgctg
     7981 ctcgttccaa gacattatct tgccaaattt ttaaatgagc aggagaacta taccagtaaa
     8041 acggaaactg gaaaatgatc cgatcatgct gggccaagag ctgtagttca gtctctatat
     8101 ctatttttcc gtctggataa cttgattcca aatgatgcca agtagctggt aaaccagcag
     8161 aagcttcttt gaaaaattgc tgatttaaag attcttcgat cgttggatgg ctaatgatga
     8221 taagtgtacg catccctttt cctccttttt agtcttcgct ttacagtgta gcataaccgc
     8281 cacaagtttc ttttgccgat gcttgtcatt ttaaccagac taatcaggtt gtccaagaaa
     8341 ctgcggtttg tttttccaac tgttttttca aggtatccaa agcaatattc ttaaaatttt
     8401 cgtttttcat acgtttacga tagttcgtag ccgtttctcc ggtactcttt ttgaacattc
     8461 ggctaaatac gactagacta ttgaacccta cttgataggc aatactttcc acggattcat
     8521 ccgtcagaga aagaagaaac gcccctcgat tgatccgtac cccgatcaaa tattccttga
     8581 tactggtacc catatgattt ttgaatactc gcgacaaatg gctccgattg accgctaaat
     8641 gctgggcgat ttcctcaaca gacaattttt cagcataatg tgcctcgatg tatttgactg
     8701 tttcaacagc taaagaggaa tgaacctttt gtacaccacc gctgaccttt ccgccatctt
     8761 ttaaaaggac tgataaaaaa cggtaggtta atgctgttaa ctgtaattca ttttccaagg
     8821 tttcttccgt atctttcatg cattctaaaa taatgtcgat atatttttgt atgtcagaag
     8881 agaccatcgt gtattggtct tctctgaaaa gcgactgccg aatgatttct tctgcagcac
     8941 tcccaccaaa tgaaagccag caatacatcc acggatcttc tccatctgat aagtaaaacg
     9001 tcgagtctcc tggacgaatc aagaacagat cacctttttt caattggaat tgttgatttt
     9061 tcacatgata agatcctttg ccttccatca cgacatgcag gatgtaatca ttgcgaatag
     9121 caggaccaaa agaatgaaac ggcatcgttt tagagaaacc acaaaaatca aaatacaatt
     9181 ctttttctac ttgttgatgt tcctttttta aaaaaatcga ttgctccacg tctatccctc
     9241 cttactcctt tagtataatg caacaaaaag ttaaataaaa gtcacagaaa tatattaaaa
     9301 aggaagcgat ttcttgttaa gtttaagtca ttaaaagatc tttgttattc gttgtaaccg
     9361 gtttacttag tattatatat tgaacaggag aggaatacag attatgaaaa gcaaaatgat
     9421 ccaacgtgtg tcctacgctt taggcgcttt tggacacgac acattctatg ttacattaag
     9481 cacttatttt atggtattcg tgacaagcca gatgttcgca ggaacagata aggcaacgaa
     9541 tgcaaaaatg attgctacag ttacaagctt agttgtaggt atccgcttag ttgaaattat
     9601 ttttgatcct ttgattggtg gcatcatcga taatactcgt acaaaatacg gtaaattcaa
     9661 accatggtta gttatcggcg gcgttgttag ttccgtactc ttggtagcaa tctttaccaa
     9721 tttctttggc ttagctactc ataatacgac tttatttatc atattatttg caatcgtttt
     9781 catcttattg gatagttttt attcatttaa ggatatcgct ttttggtcaa tgattccagc
     9841 tttgagtacg gaaacaaagg aacgtgaaaa atttgctacg tttgctcgtt tcggctcttc
     9901 tctaggtgca aatggtacta ctctagtcgt tgtccctatc gttactttct ttacctatct
     9961 tgttactcat aaacatattg aaggcgcttc aggctggttc tggttcggtg tgattgctgc
    10021 tgttatttca ggcggtactg cgattatcac tgccttgggc acaaaagaag tcgatactgc
    10081 gatccgtcag caaaatgaaa aagctgggat caaagatgtg ttcaaagcag ttgctcaaaa
    10141 tgatcagttg atgtggcttg cccttgctta tatcgcttat gcgattgcca atgcagcaac
    10201 aactgccgta ttattctact tcttcaaata tgtacttggc caaccaactg aattttggat
    10261 cgttggtcta gttgctgcct tacttggttt agtagctgtt ccactattcc caatgcttac
    10321 acgcgtcatc tctcgtcgct acgtttacct tggagggatc gcaatgatga tttcttctta
    10381 ctttttcttt acgatcgcag gttcaagttt gccaatcgtt atcatcgggc tagtattgtt
    10441 ctacttccct caacaattga ttttcttatc cgctttgatg acgatcacag actctgttga
    10501 atatggtcaa tggaaaaatg gtatccgtaa cgaagctgtt actctatcaa ttcgcccttt
    10561 actagataaa atcgctggtg ctttgtctaa cgggatcgta gggtttgtcg ctatcgcttc
    10621 tgggatgact ggtaatgcta ctgctgctga tatcactgct catggcgtaa cgactttcaa
    10681 agcttatgca ttctatgctc ctgctgcttt gatgattttc tccgctatta tttttgcttg
    10741 gaagattaca ttgacagaga aaaaacatgc acaaatcgtt gagaaattgg aaaacaaatt
    10801 ggctccagct gatacaatgg aagattaact tcacgatgca gtagttcctt cccattaaga
    10861 aaggatcttg aaaatgaaga ttacagaaaa agattttggc caaggttatc acttgataac
    10921 cttggagaac aaaaacaagc tagcactttc tatctctgat ttaggtgcta gaatcgtcag
    10981 tttaaaatca aatgatcgag aattagtttt gggatttgac accgctgaag aatatatcga
    11041 aaaagatccg tatatcggtg cttctatcgg tcgcaccgca gggagaatcg aaaatggccg
    11101 cttttctctc aatggaaaaa cttaccaatt agccacagat ccaaaaactg gacataatct
    11161 gcatggtggt gctcctggat ttgaattaaa aaaatggtct tatgtcattt tgaatggcga
    11221 aaatgaagct tctgtcattt ttactactac aagtcccgac ggagaacatg gttttccagg
    11281 tacaatggat gtggaaatcc gctatacttt gacaaaagat aatatctggc gagtgacctc
    11341 tcgcggcacc agtgaccaag atactttatt caatccgaca aatcatgttt acttcaactt
    11401 gacgggagat gcttctcagt caatcgatca acatgagctt tggctcaata gcgaagctta
    11461 tgctcctttg cgaacagata gtattcctat aggggtaaaa gaaaatgctg caggaagtgc
    11521 tttcgatttt caaataccta aaaaattagc gtccgttttt gctagtgatt tggatcaaaa
    11581 aaatcttgtt gacggaatcg atcatccatt tttcttaaaa gaaaccggct tgggtaaaga
    11641 agccgctcgc ttgactagcc ccgataaaag aatccaagta gatattgcta cagatgcttc
    11701 tagtgtggtt attttcactg caaatttcgg cacagaaaca ccagaaatgc gcaaccgaaa
    11761 actggcacat cacggtggca tcacatttga gactcaaaca gctccaggtg cagagcgctt
    11821 ctcagcattt ggttcgattc atttaaaagc cggatcagta tttgaaacag taacagaatt
    11881 caaaatcaaa acaagaaagg aataactcat catggaaatc aaaagaagct taaacacaaa
    11941 atttgccgaa acattcggac ctaaaaaaac tacacaatac ttctcaccag gacgcatcaa
    12001 tttgattggt gaacacacag attacaatgg aggccatgtc ttccccgctt ccatcactta
    12061 tggaacgcaa ggtgtagcag cacctcgaga cgataataaa atattagttt attccacaaa
    12121 ctttgacgat atcggtgtca ttagttttac tttagatgag ctagaatatg ataaaaaagc
    12181 aaactgggca aattacgtaa aaggaatggt ccttaaatta aaagaagctg gacactcaat
    12241 cgatcatggg tttgagttat tagtggaagg aacaatccct aatggtgcag gactttccag
    12301 tagcgcttca ttagaattat tagtaggtgt tgtattagaa gatctgtttg atttaaaaac
    12361 agatcgcttg gaactagtcc aaacaggtaa aaaagtagaa aatgaattca tcggtgtcaa
    12421 ttcaggtatc atggatcagt ttgccatcgg cttcggtgag ctagatcatg caatcttatt
    12481 agatacaaat acgctgaaat acgaaatggt tccagtgaaa ctagatggtt atgcaatcgt
    12541 catcatgaat acgaataagc ggcgggaatt ggcagattca aaatacaatg aacgccgtag
    12601 cgaatgcgaa gaagccttgg cacgtttgca aaaaactttg cctatctctt ctttaggaga
    12661 cttagatgaa gaagaatttg aatcaactat tgaccaaatc ggcgatgata cattgatacg
    12721 cagagctcgc catgccgttt ctgaaaatca gcggactttg aaggcaaaag ctgaattaga
    12781 agctgggaac ttagaagcat ttggtcaact gttaaatgat tctcaccact ctcttcgcta
    12841 tgattatgaa gtaactggga tcgaattaga tacactagta gatgctgctc aaaaacaaga
    12901 aggtgtctta ggtgcacgaa tgacaggagc tggatttggt ggctgtgcga ttgctcttgt
    12961 caaagaagaa aatatacctg ccttcaaaaa caatgtctat gatgaataca tgaaagttgt
    13021 cggctatgct ccggaatttt atgttgcaca cattggaaat gggacaacaa agatcgatcc
    13081 agaagctgtt catgtttgat aaaaaaatca acgatctcag ctaaaaacaa atagataaaa
    13141 ataacctctc agcaatttcc aaatgtgagc cttttgctcg tatttgaaaa ttgctgtttt
    13201 ttattgacac ggaaagagaa cgtgtgttct aattgtgttg aagaggtgat tgttatgtgg
    13261 gcgaatcaaa atccggtatt tgtttatgaa aaagaacctt caagagatat tttatgtatt
    13321 gactgcaaat ctttttacgc ctccgtagag tgcgttgaaa aggatcttga tccccttaca
    13381 actaaattag tagtcatgtc ctatccgtcc gattcgacag aaacacgggg tagcggatta
    13441 atcttggctt ccagtcctgc agcaaaaaaa gcttacggca tcaccaatat cagccgtgcc
    13501 agagatcttc ccttccccta tccaagtgat ttatatattg ttgccccaag gatggcctat
    13561 tatatggaaa agaacagaga aatcaattct atctataaaa aatatgcaga cgaagcgaac
    13621 catcatgtct attccgtcga tgaaagcttc cttgatgtaa caaatagcat ggcactcttt
    13681 ggtaaaacaa atgcgtacga catggctaaa ctgatccata aagatgtgtt tgagcagacc
    13741 ggaatctaca cgactatagg aattggcgat aacccgctac ttgctaaact agccttagac
    13801 aatggctcaa aagacggaaa gcaaatgttt gctgaatggc gttatgaaga tgtaccagaa
    13861 aagttatgga aaatctcaga tatcactgac ttttggggaa tcggcaaacg aacagcaaaa
    13921 cgtctgaaac aaatgggtat acattctgtt tatgatttag ctcacgcgga ttattatcac
    13981 ttgaaaagca agctaggggt catcggtact cagctttatg cccatgcttg gggcatcgat
    14041 cgaagttttt tgggacaaga atatacacca aaatcaaaaa gtatcggaaa tagtcaaatc
    14101 ctagatagag attatacaag acgagaagaa atcgagatcg ttatcaaaga aatggccgac
    14161 caagtcggca ctcgcttgag acgatctgca gcaaaaacac aagtagtcag cttatgggta
    14221 ggatactctt taggctatct tgatccaaat ggcaagaccg ggtttcacca gcaaatgaaa
    14281 atcgaagcga caaatgctag cagagatctt gcagatgctt tattgaccat tttccaccag
    14341 cattaccact accaggatat tcggcgtgta ggcgtgaact gttccgacct cgtatacgcg
    14401 acaggtttac agcttaacct ttttgaagat cctgaaaagc agataaatga agcgaaagtt
    14461 gattttgttg tcgatacgat tagaaaaaaa tacggctttc aatcaatcat ccatgcgcat
    14521 agtctcttgc aaggaggacg cgccatcgca cgtagttctc tcgttggcgg gcatgcaggt
    14581 ggtatgagtg gattagaagg tggaaaagat gagacggaca gcaaaaaaat tcattgacta
    14641 taacgagtat cgagatcggc catttggatt aaaatggggg actgcttttg cgatggaaga
    14701 actagtcaac ggtatcaaaa aaatgaagaa gaagcacgca aacagcctct tcctaaacaa
    14761 caaatgacat tgcaagaaat cgatagtata ctgacagaag cctttttatc tcgtaaagca
    14821 gtaatcattc agctcaattt aaaagatgaa tttggaagac tcttggatga ggtcgaaggg
    14881 cacttcattg gtgaagccta tgaagattac tttatgattg accatcagcc gatattgtgg
    14941 gaagacgtac ggtatattga gttaaaggaa acaaaaaaat ggtttgtgac atgagaggtt
    15001 gtgacagaag tgatcaactt caaaaaataa gaaaggattt ttcgaaaatt gcttcttaaa
    15061 tttttgtgga attttggctt atttttcgaa gaagcaactt ctggaacaac gtatattaga
    15121 tatcagaggt tgtgaaaaag tcgcttagtt ttgagtaaca agccaaggcg ctgatttcac
    15181 aacctctgtc tatctatcca tctattcggt aagattcatg catgacgtct tctttattta
    15241 gtatgtagag ccctttttct tctgaccatt caacataaat cagctgtttt acatctgcat
    15301 agccttcttt cttcaagttc tccataagcc atgcttcagt caattgattg gactgaagca
    15361 ctttatgctg aatttgccct tcatctacaa gcaggatcga taaagctttg tcttcatctg
    15421 attttttcaa tacactcatt tgaccggcat tctctaggat aacatgtttg gcattttcaa
    15481 gaaaaaagca ttcttgctgt cttaagattg ctcgcaactg ctccatctcg atatggttat
    15541 ttgtcaactc tgtcatgttg atgacgccgt ctttgattag aacagatggc tcgcctttga
    15601 cccaacgatt gaccttttca tttttttgga cgatacgttc gataccataa atcattactg
    15661 cccaaagcgc aatggcaaat aggacatgcc ctacatggac attgtcatca taggttgatt
    15721 cttctaagat cccacctaaa acaagcgtat agactaaatc aaacggtgtg atttctgaca
    15781 tggatttctt cccaagcaaa cgtaccacta ataataaacc gatcaacccg aacaccagtt
    15841 tcattgtaat tgaaaaaatc attttttctc cccctaacta tctgttaatt aaattatata
    15901 tgctttctca cgaacacgca tggaatatac aaggaagaaa tgtatccttt tactaaaaaa
    15961 aggactgtga cactgtagtg acccccaaaa gttagacttt tttggagtgg agaaatccac
    16021 tccttttttt tatgctctaa aatgatattt ttacacaacc gttacggttg ttttacgaaa
    16081 ttggacgaga ctttgccagt ttaatttctg ttttatccgt tcattattgt agtaatagat
    16141 atagctatcg atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat
    16201 ctcttgtttt aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt
    16261 ccgagacatg ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg
    16321 atacgcccaa ccttgatctg agtgaaacgt acgacgatag ggacaatcgt tagtggtctg
    16381 gatagcttcc tccaaacctt ccattaccgc taatgcattg ggcttttcag agatacgata
    16441 ggataaaatc tcactattga acatatccat aaaaggatct agatacaatt tcttttgacg
    16501 gataatgcct ttggcatcca cttcatggta tttaaattca gttgtatccg tcgtgatttt
    16561 ctgatggcag atactggtgt agaaacgacg atggatccga tttttggcta ctgctccgac
    16621 ttgtcctcga taagaattgt agcgccgtga tttacgtgtg tatgtcgtta cttttattcc
    16681 tagcttcttg atcaggcgct gaactttctt tttattgata gagaaaccgc ggtttcttag
    16741 ttcattggtc atccgtaaac agccataatc cttgtgcttc tcccgaactt taagcatttc
    16801 cgcttcaatc tcttgatctg gatttttacg gtccaatcgt ttttgccagt acataaagat
    16861 tgccttaggg aactttaaag tttctagaag ctcaactaat ttaaacggtc ctcggaggct
    16921 tgcgatgatt cgtgcgattg cttcattcgt cgttgttggg cttcctgttt tcgcagcttc
    16981 ctcaattctt ttaaaaaggc attttcaatt tgaagggaac ggactcgttt ctccaactcc
    17041 tcgatgcgtt cacgttcttt agacgttgct ttagttgtag attttttctt atttttgggt
    17101 tcatttttct tagacaagat aggtggacat cctttcgttt ttgagagtcc atcgatccca
    17161 ccctcacgga attgacgcat ccaactagcg atcaaattag ggttgttcat gttaagtgtg
    17221 ttggcaactt cacgatagga cagctcactt gttagatata actctatcgc atccagctta
    17281 aattgaacag aatatttttt cttttgtcgc tttcttacta gaccctcttc accaaattct
    17341 tgataggcat tgatccagcg cttcacttgt gatggagcct taatggaata tttctttgcc
    17401 agataatcac ttccgccttt accatccaag taatcatgaa cgatctttaa tttaagttca
    17461 aaagtatact tagccataaa aaataccgac ctccaaaagt tagatttttt ggtctaactt
    17521 ttgggggtcg gtacaaaact taaaagtttt agaccctttt attttcttaa tgacttcagt
    17581 cagttttgtg cgtaaagcgt gcgaaacaag aaaaccgcgc gtttctacgc gtggaagaag
    17641 tatctttagc gataaaaact cctttcgata tactagtgag tggctaccaa ccgctcatca
    17701 gatcgaaagg agttttttca tgtcaaatga cgataaaagt ttagcatata ctcgttggaa
    17761 ttgtaagtat catattattt ttacaccaaa atatcaacga aaagtaatct atgggcaatt
    17821 acgaaaagat cttggaaaaa tacttaggaa gttgtgcgaa atgaaagatg tggaaatcat
    17881 agaagcacat gcaatgctag atcatattca catgcttgtt cgagtaccac caaaaatatg
    17941 tatatccagt tttatggggt atctgaaagg tcgtagtgcg gtactaatcc acgagcagca
    18001 cgcaaatctc aaatataaat acggaaacaa atccttttgg tcgaagagat actatgtgag
    18061 tacagtcgga ttgaatcaaa agacaaatga aaaatacatc cgtgaacaag aagctgatga
    18121 tcgcattcga tatattataa gtaaaagaga gtacgtagaa ccatttaaaa aataaagaga
    18181 agtaagtgag tgaagatgag cagttggcgg tcttttaaaa atcccctttt aaggggcagc
    18241 gagtgtcgac cccttctagg gggattaaaa atacacccgt cctcacgggt gtatgggtta
    18301 ctgatagact tgattttaac tggtgtcaga aacttatgtc cagaaagggt gatgaaattt
    18361 tggcaattta cggctattca agaattagca caagcaacca agattacaaa acacaaattc
    18421 aaacattaga aaatgctggc gcagaaataa tcttcctaga aaatcactgg attatcgaac
    18481 acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca
    18541 attaagagta tatttacttc agatggtaag gcaacaaaat aaaatacaat aggcagatga
    18601 actaataaat aaaatctgta ttaaatacag ataaattaat ataaaaatca aaaggatcat
    18661 aaacttttaa caagtgatga aatgggtgga aattgattct tatagacagt ttgtctcata
    18721 acattgaagt attttgcaat tttagcattg aaacactttt ttaatcttca acgttgttct
    18781 tataaacaag atgattggga atcttttgaa ttaacactgt atagcttaga acacccttta
    18841 tagaaaacat tcgcttagca atcttaataa ttccttgtgc ccgatgtcgt ttagattcag
    18901 taggttcctg ctcatcgatc atggaaaaaa tttgaaggat cagatcctta ataaatttat
    18961 ttaagagagg atgatccatt gcttcatcca tactcgataa actagtgata atgagctaca
    19021 cgtttttctt tcaagttgat caatagcttc cacgataaaa atatctttag ataaaatcaa
    19081 gaaccgtagg aactacaggg atatttggtc aataagagcc accgctatta gtaaaaaata
    19141 agctatcaag tatgctctca tcccaagaat tgcctacttc aatcgttaaa aaccgttagg
    19201 tttagctaag tgggggtagt tcactcaata cttttatcaa taatagagcg caaaactttt
    19261 tctcttaatt caactgaatc aatattattg ttttgttcat aaccatgaac taatagatca
    19321 caaatataaa gttgtgatat ttttccagct aaagatccac cattaagaaa ttcttcaatg
    19381 gctgtttgta aaactaagtc tgcagacttt cctataggtg aatgaatata gttagttata
    19441 gcaataattt ttgcgccatt ttttttagct attttcaatg aatcataagt atctttcgtt
    19501 ttcccagata aagaaaaaat gattactaaa tcattaactg ttaataaaga agcgacttgg
    19561 gcttgaaaat gagggtctag tacagctttt gcttgaactc ctacgcgaag aaacatattt
    19621 tctaaatcaa gactagtatt tccactagat cccactccaa atatatatat gttttttgat
    19681 tgagtgatca ttttaatggc ttcattcaat ttttcttcgt ctaaaagcct aatagtggaa
    19741 tagagagctt ctgttaaact attagcaatt tcatcatagt actttacact agtcggatgt
    19801 tctttttttt gactaaagtc ttcctttgca atttcaattt ttaaatcaga aaaaccagaa
    19861 aaaccaattt tttgacaaaa gcgaataata gtagcatcgc caacttttgt gtttttctta
    19921 atatcactca tcgtactata aataatagtt ttcccagaat ttaaaataaa atcagcgact
    19981 tttttttcag acttcgttaa aaatggataa ttttctttaa ttaaatagat ataactcata
    20041 caaactcctc tacaatttca acttttctcc attatactcc aaaataataa aaaaaactcc
    20101 taaatactcc atttttatct ttacttttat gaaagcggat gctatattgt ttttggagtt
    20161 gttatccaaa aataataaaa aattggagtg tttaggataa tggataaaaa gaggttttta
    20221 gatcttattt caaaaggttt gattgtttcc tgtcaagcat tacctggtga accattttac
    20281 aaagaggaag aaagtttaat gccttatttt gctttggctg ctcaaagggc tggagcagtt
    20341 ggtatacgtg caaattctgt tcgagatatt attgaaatta gaaagtgtgt atcattacca
    20401 atgattggaa ttattaaaag agattatcca ccagaatctc cattcattac tgccactatg
    20461 agagaagtag atgaattagt agaaaccggt gttgaggtaa tcgctcttga ttgcactctt
    20521 agaaatcgac atgatggact atctataaat tcttttatta ctaaaataaa agaaaaatat
    20581 cctcatcagt tatttatggc agatatttct acatttgaag aaggcaaaaa tgcatataaa
    20641 gcaggaatag attttgtagg aactacttta agtggatata ctgaggaaag taaaaaacaa
    20701 gatgggccag atatagattt gattaaaaaa ttggtagaag aagggatttg tgtaattgct
    20761 gagggaagga ttcatactcc agaacaagca aaagagataa aaaaaatagg agttgcaggt
    20821 attgttgtgg gtggtgctat tactcgacca caggaaattg catcaagatt tgttaaagca
    20881 ttaagttagg gggagaaata aaatgtttaa aaaattttca caattaggac gcgctttcat
    20941 gttaccaatt gctatattac cagtagccgg attactattg ggactgggag gagcgttaac
    21001 aaatgagagt gctataaatg cttatccatt tttagatcaa ccgtggttgc atacaatttt
    21061 aagtattatg agttatgctg gaaatgcagt ttttgcaaac ctagcactaa tttttgcaat
    21121 tggtattgct gtaggacttg caaatggaga taaaggaaca gctggtttag ctggtggagt
    21181 agcttatcta gtatatactg caactattag tgggtttctt gaactttttt cagcaaaaga
    21241 tgcaacacta gatacaggtg tagtgggatc aattgttgtt ggtggtactg ttgcattttt
    21301 acacaatcgt tatagaaaaa ttgaattacc acaattctta ggtttttttg gaggatcaag
    21361 atttattccg attgtttcta gtttagctgc aatcattatt ggttctttct tttatttaat
    21421 ttggccacca attcaaaatg gtttagtagt tgctggtgaa caaattgccc aaatgggtag
    21481 tttaggaact tttttatatg gttttttatt gcgtttaaca ggtgctgttg gactacacca
    21541 caccatttac cctttgttct ggtatacatc tttaggtggt accgaaactg tagctggatc
    21601 aacaattgct ggagcccaaa atattttctt tgcccaacta gctgatccaa accacactgg
    21661 tctgtttact tatggtacta gattttttgc aggaagattt gcaacaatga tgtttggatt
    21721 accagcagca tgttatgcaa tgtatcgtgc aattcctaag aaaaatcgta aaaagaatgg
    21781 tgggctttat tttagcgggg cccttacatc atttttaact ggaattactg aaccagttga
    21841 atatatgttt ttatttgttg ctccttggct ttatgtgatt catgcattct tagatggatt
    21901 atctttttat tttgctgata tcttaaatat tcgtgtaggg aattcctttt caggtggttt
    21961 gatcgacttt ttattattcg gtgtacttca agggaatgac aaaactaatt ggttaaaatt
    22021 aattcctttt ggtattgcat gggcatttat atatttcttt gtttttacat tctttatcaa
    22081 aaaatttaag gtagctattc ctggaatgga agaagaatca gaagttcatt ctgaaaaagt
    22141 aataaacaaa aatacatcat tacatgaaga gtctcttatc attattgaag ctcttggtgg
    22201 aaaagaaaat attgagtctg ttgaagcttg tgcaactcgt ttgcgtgttg ctgttacgaa
    22261 tggggagatt attcaaaaac aaacaataaa agacactggt gcaactgctg tcttcgaagt
    22321 gaagggtggc atccaagctg tttttggagg gaaagctgac ttattaagtc aagaaatcaa
    22381 tcaaatatta ggaaaagata attaggaggt ttttaatatg tttgaaattt tcaaaaagaa
    22441 aaaacaagaa actttttatg caccttgttc cggaaaaatc atttccatca cagaagtaaa
    22501 tgatcctgtc ttttcacaaa aattactagg tgatggtttt gccgtcatac ctgatggttc
    22561 aaaaatttat gctccattgg ctggtcgagt aacaaatata tttcctacta aacatgctat
    22621 taatataaaa agcaactcag gcattgatta tttgattcat attggaattg atacagttga
    22681 attacaagga gagccttttt cgatttgtgt tcaagaaaat gatgaggtta ctcctgaaac
    22741 ctcgttggtt gatgtggatt ttgacaaaat aataaaatct ggtaaagata cttctgttat
    22801 agttgttttt ccggagaaaa atcagttcaa tgatttaata atagataatg gtcgaattgc
    22861 tcatgggaat tcatgtggaa aaatcacttt ttaaaaaagt gtatataaaa agctataaaa
    22921 taagctaaat aggatatttg tcaaatgaag ttggtgaaca gaaagaactg ttttcttt
//
LOCUS       DAYYNQ010000044        22638 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000044 DAYYNQ010000000
VERSION     DAYYNQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.044"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHBJ1_001546"
     CDS             complement(227..1114)
                     /locus_tag="ACHBJ1_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430486.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHBJ1_001547"
     CDS             complement(1111..1980)
                     /locus_tag="ACHBJ1_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430487.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHBJ1_001548"
     CDS             complement(2117..3403)
                     /locus_tag="ACHBJ1_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430488.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKDYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGTVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHBJ1_001549"
     CDS             3676..4254
                     /locus_tag="ACHBJ1_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM9430489.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHBJ1_001550"
     CDS             4254..5183
                     /locus_tag="ACHBJ1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430490.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHBJ1_001551"
     CDS             5180..6916
                     /locus_tag="ACHBJ1_001551"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9430491.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHBJ1_001552"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHBJ1_001552"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM9430492.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHBJ1_001553"
     CDS             complement(7812..8321)
                     /locus_tag="ACHBJ1_001553"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM9430493.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHBJ1_001554"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHBJ1_001554"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM9430494.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHBJ1_001555"
     CDS             complement(9622..10713)
                     /locus_tag="ACHBJ1_001555"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM9430495.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHBJ1_001556"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHBJ1_001556"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM9430496.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHBJ1_001557"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHBJ1_001557"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM9430497.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHBJ1_001558"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHBJ1_001558"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM9430498.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="ACHBJ1_001559"
     CDS             complement(14676..15650)
                     /locus_tag="ACHBJ1_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM9430499.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="ACHBJ1_001560"
     CDS             complement(15631..17514)
                     /locus_tag="ACHBJ1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM9430500.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="ACHBJ1_001561"
     CDS             complement(17532..19757)
                     /locus_tag="ACHBJ1_001561"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9430501.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="ACHBJ1_001562"
     CDS             19872..20906
                     /locus_tag="ACHBJ1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430502.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="ACHBJ1_001563"
     CDS             complement(20973..21986)
                     /locus_tag="ACHBJ1_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9430503.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="ACHBJ1_001564"
     CDS             complement(22064..22252)
                     /locus_tag="ACHBJ1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM9430504.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6449 a         4862 c         4096 g         7231 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgttcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataatctt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aactaaaa
//
LOCUS       DAYYNQ010000045        22541 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000045 DAYYNQ010000000
VERSION     DAYYNQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.045"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHBJ1_001565"
     CDS             complement(<1..210)
                     /locus_tag="ACHBJ1_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430505.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(301..753)
                     /locus_tag="ACHBJ1_001566"
     CDS             complement(301..753)
                     /locus_tag="ACHBJ1_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM9430506.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(766..1176)
                     /locus_tag="ACHBJ1_001567"
     CDS             complement(766..1176)
                     /locus_tag="ACHBJ1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430507.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1451..1663
                     /locus_tag="ACHBJ1_001568"
     CDS             1451..1663
                     /locus_tag="ACHBJ1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM9430508.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1666..1878
                     /locus_tag="ACHBJ1_001569"
     CDS             1666..1878
                     /locus_tag="ACHBJ1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430509.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2018..2173
                     /locus_tag="ACHBJ1_001570"
     CDS             2018..2173
                     /locus_tag="ACHBJ1_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430510.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2170..2394)
                     /locus_tag="ACHBJ1_001571"
     CDS             complement(2170..2394)
                     /locus_tag="ACHBJ1_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9430511.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2472..2660
                     /locus_tag="ACHBJ1_001572"
     CDS             2472..2660
                     /locus_tag="ACHBJ1_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430512.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2799..2927
                     /locus_tag="ACHBJ1_001573"
     CDS             2799..2927
                     /locus_tag="ACHBJ1_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430513.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2986..3321
                     /locus_tag="ACHBJ1_001574"
     CDS             2986..3321
                     /locus_tag="ACHBJ1_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430514.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3554..4495
                     /locus_tag="ACHBJ1_001575"
     CDS             3554..4495
                     /locus_tag="ACHBJ1_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM9430515.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4497..5387
                     /locus_tag="ACHBJ1_001576"
     CDS             4497..5387
                     /locus_tag="ACHBJ1_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9430516.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5430..6230
                     /locus_tag="ACHBJ1_001577"
     CDS             5430..6230
                     /locus_tag="ACHBJ1_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM9430517.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6245..7096
                     /locus_tag="ACHBJ1_001578"
     CDS             6245..7096
                     /locus_tag="ACHBJ1_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430518.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7135..7296
                     /locus_tag="ACHBJ1_001579"
     CDS             7135..7296
                     /locus_tag="ACHBJ1_001579"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430519.1"
                     /translation="MLQIIGDRDMDELSQQESRMVRKISKLIIYRDTERFLTVLGNVY
                     ENPELLEVE"
     gene            7297..7758
                     /locus_tag="ACHBJ1_001580"
     CDS             7297..7758
                     /locus_tag="ACHBJ1_001580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430520.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7784..8236
                     /locus_tag="ACHBJ1_001581"
     CDS             7784..8236
                     /locus_tag="ACHBJ1_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430521.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8342..8536
                     /locus_tag="ACHBJ1_001582"
     CDS             8342..8536
                     /locus_tag="ACHBJ1_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430522.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8567..8953
                     /locus_tag="ACHBJ1_001583"
     CDS             8567..8953
                     /locus_tag="ACHBJ1_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430523.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8950..9153
                     /locus_tag="ACHBJ1_001584"
     CDS             8950..9153
                     /locus_tag="ACHBJ1_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9430524.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9150..9446
                     /locus_tag="ACHBJ1_001585"
     CDS             9150..9446
                     /locus_tag="ACHBJ1_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430525.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9522..9938
                     /locus_tag="ACHBJ1_001586"
     CDS             9522..9938
                     /locus_tag="ACHBJ1_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9430526.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10199..10336
                     /locus_tag="ACHBJ1_001587"
     CDS             10199..10336
                     /locus_tag="ACHBJ1_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430527.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10746..10976
                     /locus_tag="ACHBJ1_001588"
     CDS             10746..10976
                     /locus_tag="ACHBJ1_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430528.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11135..11968
                     /gene="terS"
                     /locus_tag="ACHBJ1_001589"
     CDS             11135..11968
                     /gene="terS"
                     /locus_tag="ACHBJ1_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM9430529.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11961..13376
                     /locus_tag="ACHBJ1_001590"
     CDS             11961..13376
                     /locus_tag="ACHBJ1_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM9430530.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13388..14917
                     /locus_tag="ACHBJ1_001591"
     CDS             13388..14917
                     /locus_tag="ACHBJ1_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM9430531.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14835..15887
                     /locus_tag="ACHBJ1_001592"
     CDS             14835..15887
                     /locus_tag="ACHBJ1_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM9430532.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15889..16206
                     /locus_tag="ACHBJ1_001593"
     CDS             15889..16206
                     /locus_tag="ACHBJ1_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430533.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16333..16956
                     /locus_tag="ACHBJ1_001594"
     CDS             16333..16956
                     /locus_tag="ACHBJ1_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM9430534.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16971..17861
                     /locus_tag="ACHBJ1_001595"
     CDS             16971..17861
                     /locus_tag="ACHBJ1_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM9430535.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17884..18099
                     /locus_tag="ACHBJ1_001596"
     CDS             17884..18099
                     /locus_tag="ACHBJ1_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430536.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18111..18452
                     /locus_tag="ACHBJ1_001597"
     CDS             18111..18452
                     /locus_tag="ACHBJ1_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430537.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18452..18823
                     /locus_tag="ACHBJ1_001598"
     CDS             18452..18823
                     /locus_tag="ACHBJ1_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430538.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18816..19211
                     /locus_tag="ACHBJ1_001599"
     CDS             18816..19211
                     /locus_tag="ACHBJ1_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM9430539.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19213..19590
                     /locus_tag="ACHBJ1_001600"
     CDS             19213..19590
                     /locus_tag="ACHBJ1_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM9430540.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19591..20199
                     /locus_tag="ACHBJ1_001601"
     CDS             19591..20199
                     /locus_tag="ACHBJ1_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM9430541.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20199..20540
                     /locus_tag="ACHBJ1_001602"
     CDS             20199..20540
                     /locus_tag="ACHBJ1_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430542.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20782..22541
                     /locus_tag="ACHBJ1_001603"
                     /pseudo
     CDS             20782..22541
                     /locus_tag="ACHBJ1_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8143 a         3291 c         4642 g         6465 t
ORIGIN      
        1 attaatatca ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt
       61 acttaatata aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc
      121 taattcataa gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat
      181 taataaaata agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta
      241 ttgtagagtt tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt
      301 ttaatgttgt aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg
      361 gaactgactc atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc
      421 aaacatatta gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa
      481 agctgctgtg caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa
      541 tcttttcata gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg
      601 tatcgtttgt cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata
      661 tttgattatt ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat
      721 ttcttctaat atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt
      781 tttaggagtg aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc
      841 atcgtataat ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc
      901 atctgaattc aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt
      961 ttctgttaaa tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac
     1021 attaaagtaa tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct
     1081 agacatgctt ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg
     1141 ttcttttaat aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata
     1201 ccgacaaaaa aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat
     1261 actagttttg taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt
     1321 ctccccagac atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag
     1381 catatttgca gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg
     1441 ggtatgaagc atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag
     1501 tcaaagagag ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat
     1561 cgaaaaaaga aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt
     1621 agaagtagat tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg
     1681 agcaaagcgg agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg
     1741 aaggaattta aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata
     1801 acaattgatg tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga
     1861 gttagagctg tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt
     1921 gctgctctcc tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct
     1981 tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg
     2041 gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt
     2101 agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc
     2161 taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat
     2221 ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc
     2281 gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc
     2341 tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt
     2401 cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa
     2461 ggagaagtga tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac
     2521 aacgcaggaa acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag
     2581 ataggcaagc tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
     2641 atagggagat tagattatga agaagacaga cacacttttt ataggattca ttttggggtt
     2701 attagtgatt gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt
     2761 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     2821 aaatcggtaa agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca
     2881 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag
     2941 caaagagtca catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt
     3001 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
     3061 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
     3121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
     3181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
     3241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
     3301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
     3361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
     3421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
     3481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc accaattgat
     3541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga
     3601 ccgtaagaaa ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa
     3661 atctccttat caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag
     3721 tgagcccgct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
     3781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
     3841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc
     3901 taatcaattt ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca
     3961 agttcaacat tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg
     4021 tggtcaaaaa tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac
     4081 tgaacaatta gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga
     4141 tggtagtcaa gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca
     4201 aacgacgtta ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac
     4261 taagaaagaa attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg
     4321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg
     4381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     4441 agagatttat aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg
     4501 caacaaatga atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag
     4561 gtcaattagg acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg
     4621 tgcttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
     4681 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
     4741 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
     4801 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
     4861 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
     4921 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
     4981 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
     5041 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
     5101 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
     5161 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
     5221 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
     5281 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
     5341 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
     5401 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
     5461 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt
     5521 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
     5581 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
     5641 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
     5701 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
     5761 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
     5821 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
     5881 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
     5941 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
     6001 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
     6061 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
     6121 tgcgagaaaa atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa
     6181 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
     6241 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
     6301 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
     6361 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
     6421 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
     6481 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
     6541 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
     6601 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
     6661 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
     6721 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
     6781 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
     6841 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
     6901 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
     6961 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
     7021 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
     7081 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
     7141 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
     7201 aagataagta agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac
     7261 gtctacgaga atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt
     7321 tgcggtagta gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt
     7381 cgtcagcagt atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc
     7441 gcagactttc gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg
     7501 ccacacttaa gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac
     7561 gaactttggc cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct
     7621 tcaggaacat taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag
     7681 gctattggag aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt
     7741 atgaaaaaat tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa
     7801 taaatgtttt ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa
     7861 aaatattgaa gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac
     7921 gagatacaca ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc
     7981 gaaaatcgac aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga
     8041 tgattccagt ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga
     8101 aaaactagga agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga
     8161 tggggctgag tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca
     8221 aacaggtagt atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga
     8281 tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt
     8341 catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt
     8401 cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat
     8461 tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt
     8521 agtgacttgc gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca
     8581 ccaactcata tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg
     8641 gtttccaatt gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga
     8701 agatttaaga caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga
     8761 gtggttgaaa agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt
     8821 ttctgataaa aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt
     8881 ggcttgtaaa caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga
     8941 ggaagcggaa tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat
     9001 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     9061 gcaagtcagt ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta
     9121 aatgatgatc gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta
     9181 gtcatggtat cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga
     9241 aatcaaaatt caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt
     9301 ttcgtggata tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga
     9361 tggaatgcga taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg
     9421 atatggaact aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9661 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9721 cggagagaga cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac
     9781 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9841 gaggatacgg agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag
     9901 aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga
     9961 tagtgtaggg tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat
    10021 aggtctgaga caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct
    10081 tcgatagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
    10141 gttcttatta tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat
    10201 ggataaagaa atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa
    10261 caaggctagt gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa
    10321 aatccaagtt tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta
    10381 tcagaaaaat ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt
    10441 taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac
    10501 aaaataaaaa atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa
    10561 gacgcaaagg tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga
    10621 aaggactagt acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta
    10681 ggttgctatt acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct
    10741 acataatgag aaactactgg tatatatcgc taactaatga atatcctcga accattgatg
    10801 attgttcagt gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa
    10861 tgaccagaga agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg
    10921 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10981 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    11041 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    11101 caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    11161 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11221 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11281 cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11341 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa
    11401 gagaaataag aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac
    11461 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11521 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11581 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11641 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11701 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11761 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11821 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11881 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11941 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    12001 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    12061 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    12121 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12181 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12241 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12301 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12361 cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12421 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12481 ttgataataa gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt
    12541 cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12601 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12661 ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg
    12721 aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg
    12781 ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12841 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa
    12901 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12961 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    13021 ttatagaaga caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    13081 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    13141 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13201 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13261 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13321 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13381 tgagtttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13441 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc
    13501 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13561 caatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13621 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13681 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13741 aaatgatata gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga
    13801 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13861 agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13921 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13981 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    14041 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    14101 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    14161 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14221 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14281 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc
    14341 tactgcaaaa gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat
    14401 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14461 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14521 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14581 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14641 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14701 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14761 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14821 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14881 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14941 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    15001 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    15061 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    15121 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15181 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15241 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15301 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15361 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15421 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15481 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15541 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15601 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15661 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15721 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15781 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15841 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15901 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15961 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    16021 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    16081 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    16141 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16201 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16261 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16321 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16381 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16441 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16501 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16561 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16621 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16681 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16741 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16801 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16861 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16921 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16981 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    17041 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    17101 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    17161 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17221 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17281 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17341 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17401 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17461 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17521 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17581 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17641 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17701 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17761 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17821 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17881 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17941 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    18001 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    18061 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    18121 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18181 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18241 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18301 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18361 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18421 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18481 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18541 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18601 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18661 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18721 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18781 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18841 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18901 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18961 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    19021 gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    19081 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    19141 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19201 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19261 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19321 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19381 ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta
    19441 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19501 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19561 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19621 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19681 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19741 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19801 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19861 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19921 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19981 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    20041 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    20101 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    20161 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20221 ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg
    20281 aatttctacc aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg
    20341 ctgtagatgg aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata
    20401 ttttgtttga aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa
    20461 ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata
    20521 cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac
    20581 ttatcgagaa ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20641 tgaacgtact gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga
    20701 gcatgaggat gttgagtact acgataactt cacccaattt taggaggtga tactttgaat
    20761 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20821 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20881 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20941 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    21001 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    21061 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    21121 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21181 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21241 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21301 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21361 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21421 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21481 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21541 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21601 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21661 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21721 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21781 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21841 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21901 gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21961 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    22021 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    22081 acttggaatt ctgctaaata aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    22141 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22201 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22261 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22321 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22381 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22441 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22501 acctccatga tttctggtgg atgggaagaa acaaaaaata a
//
LOCUS       DAYYNQ010000046        22304 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000046 DAYYNQ010000000
VERSION     DAYYNQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.046"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..334)
                     /locus_tag="ACHBJ1_001604"
     CDS             complement(<1..334)
                     /locus_tag="ACHBJ1_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430543.1"
                     /translation="MKNLTQNLLKKDNSSILKIELKKENNKSMKISKKAFLLVVLILL
                     STLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFL
                     TIIPAVVFY"
     gene            396..2033
                     /locus_tag="ACHBJ1_001605"
     CDS             396..2033
                     /locus_tag="ACHBJ1_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430544.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2178..2918)
                     /gene="truA"
                     /locus_tag="ACHBJ1_001606"
     CDS             complement(2178..2918)
                     /gene="truA"
                     /locus_tag="ACHBJ1_001606"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM9430545.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3109..3744)
                     /locus_tag="ACHBJ1_001607"
     CDS             complement(3109..3744)
                     /locus_tag="ACHBJ1_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM9430546.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3891..5111)
                     /gene="sstT"
                     /locus_tag="ACHBJ1_001608"
     CDS             complement(3891..5111)
                     /gene="sstT"
                     /locus_tag="ACHBJ1_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM9430547.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5330..6328
                     /locus_tag="ACHBJ1_001609"
     CDS             5330..6328
                     /locus_tag="ACHBJ1_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430548.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            6524..8359
                     /locus_tag="ACHBJ1_001610"
     CDS             6524..8359
                     /locus_tag="ACHBJ1_001610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9430549.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8419..9135)
                     /locus_tag="ACHBJ1_001611"
     CDS             complement(8419..9135)
                     /locus_tag="ACHBJ1_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9430550.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(9362..9904)
                     /locus_tag="ACHBJ1_001612"
     CDS             complement(9362..9904)
                     /locus_tag="ACHBJ1_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM9430551.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9909..10136)
                     /locus_tag="ACHBJ1_001613"
     CDS             complement(9909..10136)
                     /locus_tag="ACHBJ1_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM9430552.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(10215..12095)
                     /locus_tag="ACHBJ1_001614"
     CDS             complement(10215..12095)
                     /locus_tag="ACHBJ1_001614"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9430553.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12284..12898)
                     /locus_tag="ACHBJ1_001615"
     CDS             complement(12284..12898)
                     /locus_tag="ACHBJ1_001615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430554.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13040..13600
                     /locus_tag="ACHBJ1_001616"
     CDS             13040..13600
                     /locus_tag="ACHBJ1_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430555.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13905..14219)
                     /locus_tag="ACHBJ1_001617"
                     /pseudo
     CDS             complement(13905..14219)
                     /locus_tag="ACHBJ1_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14473..16108)
                     /locus_tag="ACHBJ1_001618"
                     /pseudo
     CDS             complement(14473..16108)
                     /locus_tag="ACHBJ1_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16547..17416)
                     /gene="scrK"
                     /locus_tag="ACHBJ1_001619"
     CDS             complement(16547..17416)
                     /gene="scrK"
                     /locus_tag="ACHBJ1_001619"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9430556.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(17436..18557)
                     /locus_tag="ACHBJ1_001620"
     CDS             complement(17436..18557)
                     /locus_tag="ACHBJ1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9430557.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(18559..18954)
                     /locus_tag="ACHBJ1_001621"
     CDS             complement(18559..18954)
                     /locus_tag="ACHBJ1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9430558.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(18973..19770)
                     /locus_tag="ACHBJ1_001622"
     CDS             complement(18973..19770)
                     /locus_tag="ACHBJ1_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9430559.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(19772..20560)
                     /locus_tag="ACHBJ1_001623"
     CDS             complement(19772..20560)
                     /locus_tag="ACHBJ1_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9430560.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(20574..21050)
                     /locus_tag="ACHBJ1_001624"
     CDS             complement(20574..21050)
                     /locus_tag="ACHBJ1_001624"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9430561.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(21122..>22304)
                     /locus_tag="ACHBJ1_001625"
     CDS             complement(21122..>22304)
                     /locus_tag="ACHBJ1_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9430562.1"
                     /translation="EVQLVNRFIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALI
                     LAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILL
                     VDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNV
                     DYIYESSNIKKDMIVCSCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYL
                     SELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNIRKFSNQLNRHFNDNELKVLNN
                     NILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLI
                     ERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKR
                     A"
BASE COUNT         7072 a         4367 c         3410 g         7455 t
ORIGIN      
        1 cataaaaaac gactgcagga attattgtaa gaaaaggata aaaatagttc agtccatctc
       61 cataattgtt aaatgttgta tagttgattg gcccttcaaa aatagaagat aaccctttga
      121 ttcgtgaaag atgaaaagat aaatcacctg ttgtataaat ttcttgagca tttctcataa
      181 aatttactga atataaagta ctaagtaaga taagaactac taaaagaaat gcttttttac
      241 ttattttcat agatttgttg ttctcctttt ttaattctat tttcagtata ctagaattgt
      301 ctttttttaa taagttttgc gttaaatttt tcatagcact agtatgatat actagaaaag
      361 aatcaatttt tattactttt aaataggaga aaaaaatgaa aaaaaacaaa aaccaaataa
      421 tagatatcat ttttatgtta tttctaagtt ttgcttacgt catccctcta ttcatgtcta
      481 agggaatcta tcatagtgtc aatcaggata cttactttca tttatctcga atcataggac
      541 tagataatgt atggagttct ccagtaaact tcaataactt cgatcatcat ggaacaatga
      601 tgaatatttt ttatccatgg cttactcttt atccagcttt tttattttat aaaatgatgg
      661 gaaatcttgt tttaagttat aatatctact atttttttat cactttttta acaatggtcg
      721 tttcttattt ttctatgaaa caaataaaaa acaatcgtta tatttcgctg ttattttcga
      781 ttatttatac attttccgcc tatcgtgcca ttgacatatt ccatcgtgct tcattgggag
      841 aagctgtcgc tttaacattt ttgcctttga tactaatggg gtgttacgaa atttatatta
      901 gagattacca aaaatggtac tggttgtcta ttggcatgac gctagtagtg tatactcatt
      961 tactttctgt cgctatggtt tctgtattta ttggaggaac gttgtttctt tctttttatt
     1021 tttgggacca aaaaatcgct agactcttaa gtcttttaaa agctaccgtc ctcacctttt
     1081 ttttaagtgc gggattctta attcctttta ttcaacaatc tagagcacaa gaattgaaag
     1141 tcccactggg aaaagaatta agtggtatgg ctcctagtga tatgctgaca catattttaa
     1201 ataataacta taacaactat actattggat tattcttatt tctcggattg attggtgctt
     1261 ttatttttat aaaaaaatta actgctgatg atttatttat tttctttctt ggtgtatttg
     1321 ttcttttttg ttcaaccaat ttatttcctt ggcaactttt taaccataca ccagttaaga
     1381 gtctacaatt tgtctggcgg ttaaatggtt tttcttcttt atttatagcc tatacgatga
     1441 gtatagtgat ctattatatt ttttctacta aaaataattc atggaaaata atggtcttta
     1501 cgtttcttgc actaatattg catctctcag gagtaagcaa ttctatccat gctaacaaag
     1561 attcattaac acttatagca cctgaagacg ctgttgctat agctgaaaat tacaatcata
     1621 ctgattatgc aaacaaagag tcaatctatc atcctgatat gattaataat gatcaatatt
     1681 tactaggtaa ccaggaaata aatccaacaa ctcattttat gagtaacaaa cttacaattg
     1741 aattagataa ttcgaataat aaaaatactg tcttgacgac acctatttat cgttataaag
     1801 ggcaagtggc ttctattaat ggcaaactag tacaaacaaa attatctaag tttggcacaa
     1861 ctgaattaac tattcctcca ggtataaata aagtcgttat tacttatcag tacacaaaac
     1921 tagcaattgc ttcaagatat ttatctatcg ttactttgat tttattttta ctttatcgat
     1981 ttactttttc taaacaaaaa caaccgagaa gagaagtaca acattctcac tagattttta
     2041 tttaagaaag actttttagc tgtacacata aacaggctaa aaagtctttt tttatatttc
     2101 tttaatttgc ttcatacatt atttaacatc gatatttata ctgtgaaaca tgcgtttcat
     2161 ccattatcaa agtattttta tcgataataa acttcatcca aaaacagacc ttgtgcaggg
     2221 acggtttctc ctgcatgttg ccgcactttg ctttcaaata tttcatcgat tgtttctatc
     2281 ggcatggcac ctgtgccgat ttctagtaat gtccccatga taatccgcac cattttatat
     2341 aagaatccgt ctccgacaaa agtaaaatga agcatttctc cctttcgttc gatagataat
     2401 tcatcaatcg tacgcactgt ggattttttt gattttttca atgcagaaaa accaatgaaa
     2461 tcatgtgtac ccactaattt ttcgcaagct gcattcagct tgtccatatc caactcttga
     2521 gggaaataaa agctgtggtt tcgctcaaat gctgaaggaa tcacgttgtt ccaaacataa
     2581 tagctgtatt ttttgcccac cgcattatat cttgaatgga agcgctcagg catttcttcg
     2641 attcttttga tgacaatatc ccgcggaagg taacgattca taaagtcgat catttctgcc
     2701 agtccaattt ctgtgtttgt tttaaaattt gctacttgtc ctcgcgcatg ggtgccagca
     2761 tctgttcttc ctgatccaat aatttctatc tcttcatttg tcatctgatg caatactttt
     2821 tcgatttttc cttgaatggt tttatcgtca tctccaagtc tttgccatcc tgaatatctt
     2881 tttccgtcat attcgattgt caacttgatg tttctcatat tttagtggct cctctgtctt
     2941 ccatttggtc ttctctctac ctttttaaga ttgtagcaca aaatatagct ccttgcttct
     3001 ctaagacaga agctatacaa gaaaactgtg gaaatatata tttcagaaaa acaaaaaaca
     3061 gccaccggat gaagtatccg ctgactgttc aatttgacgg ctcaagtttt actgtttgag
     3121 atgcaagata ctcaaccagg caatgggcac ataattaaag aaaaaactga ttctttggca
     3181 aaaacctatt ttattcagta atggccaaga atttatagaa ggaattcttg ccagtccata
     3241 aatcgccgca aaaagcaaac tgatcacaga cagtccaagg tataagtggc ttacgtccat
     3301 ttgcccattt tttcgataga tcaagaaaag aagaactgga aacaataaaa aaccactgta
     3361 acctaaacct gatccgatat tatggaccca tgtcgagaca ttccacgaag attcatgggt
     3421 attgatactg aacaaacctg taaagataca atcacctata ccatagaacg cgatggctgc
     3481 tgtaactagg gtagctaaca cacgagatgt actaacaaat gcagcataca atgcaggcaa
     3541 tgacaggatg aaaaagatcc ctgaaaccac agaccaaata agaaacgctt ttcttacagg
     3601 actggaaacc tctccaaata cactgatcac gctcttcatt tgatctaatt caggataata
     3661 caatcctaat acgtacggag tcaaaaaatc actcagcacg cctaataata aaaagaaaaa
     3721 accaaattta cgtaaaaaat ccatgatacg cctccaataa caattaccta tgcttttaat
     3781 tgttattata aacgacttta tcttgattta acagaaacct ctatttacac gaacatctat
     3841 ataactcatt aaaaactcga atcattttaa aaatactaaa tatctaacgt tcaacgttca
     3901 cttaatccat ctaaacaatc tttcaatttg attttttccc cacttctttt acgcgcgcct
     3961 agctctccta ttgcagtaaa caacagatca cttgacgaat ttaaggcagt ttccattgaa
     4021 tcctggataa caccgataat aaatccaata ccgacgattt gcatcgcgac atcattagag
     4081 ataccaaaca aactacatgc taatggaatc aataacagtg aaccgcccgc aacacctgaa
     4141 gctccacaag cagctaaagc agacaaaata cataaaagga ctttcaaaac aaaaggtgct
     4201 tcaatcccta aagaatgtac agcagtcaac gtcatcaagg aaatggtaat cgcggcaccg
     4261 cccatattga tcgtagcacc taacgggatc gatatggaat aagactcttt tgtcaatttc
     4321 aatctttcag ccaacatcat gtttatcggg atatttgcgg cagaacttcg ggtaaagaaa
     4381 gcgggaagtg cgctttcttt caaacaaaag aaaatcaatg gatagggatt ttctttaata
     4441 aacaaaaacg tgaggaatgg gtacacaact aatgcgacaa acagcatcgt accgatcaaa
     4501 acaagtaaaa gctgcagata ctctgccaat ccagcgattc cagtcgtagc aattgattga
     4561 taaaccaatc ctaaaatccc tatcggagca aatgcaataa tcacttgtac gacttgtgac
     4621 aacgctatag atatctgatc caccaactct tttgtcgatt ctgaacgtgc tcgaaaagct
     4681 agtcccaaca atacagccca gaataagacg ccaaggtaat tcgcatcaat aagagattga
     4741 acaggatttt gtgtgacatt cgtcaacata gtagataaaa cactttccaa actttgcggt
     4801 gctttttcag atactgtttc ttgtaaagta ataggaacag taaaaattga acttacaata
     4861 actgctacaa gtgcagaaag caatgttccc actaaatata agatcaaaat aggcttcacg
     4921 aatgtcttat tcccaatttt atgcttggca attgacgcca tcgtcaagaa aaaaaccaga
     4981 ataggtgcga ttgccttcaa gcaactgaca aacaacgttc ccaatattga aataaatgtc
     5041 catgtgggga aaaagatacc aaacaatatt ccgactaaaa taccaacaac aatacgttga
     5101 actaacgaca tatccaaaaa cttctctaac aaatgaccac cctttcacaa ttttaacaat
     5161 cattgtatca ttttatgcaa agaaactaaa ggtataattt tctaaaaatt ctaacaatac
     5221 aaacaaccat ttcttgctaa ttttgtactt tttctgtcta cacgttgttt tttgagatat
     5281 tctaagttag gatgttatta atgggcaact tatcaggaaa cgttttctta tgtttttgtc
     5341 caattttttt gtgaggtgtt tattcttgaa aaaaataaag aaggagagtt atggcttcct
     5401 gacaatcttt gtcgcttgta taatgatggt tttacagcgt atattgattt cagcagtcac
     5461 tattcctaca tatgcaacaa accctggtcg attctacatc tatacatttt tacaaacttc
     5521 cgttgttatt ggtgcaaatt tgatcccact ttacatgggt taccaatatc aaaaaatcgc
     5581 ccgtttaaaa gttttacatt atttaagtcg ttttgccttt atattcctaa tagccacact
     5641 agtttgcaat atcttttttt acgtacaagc tggcagcttg aatattcgtg attattggct
     5701 tatcctaaca cctatcagtc agaattactt tacttatgct gtttcttgta ctttgctttt
     5761 ttgtagtatt ccttatacag ttggattttt aaataaatta tcgaaaacaa caattaaata
     5821 tctactatta tttttaacaa ttggattagt tggctgctct actctattta ataaagaccc
     5881 gtttgcatta cagaatggac agagtatttt atggattttt tatttatttt taatgggtta
     5941 tggtttgaaa gaatggaact ggaaagaaaa ggtccgctat aagcttcctc aagtatttat
     6001 cagcttgatt gttttattcg gtttgattat tttgatgaca caaatttcac tcatcatcag
     6061 aggaaataca gatacagcgc tgaggttttc taaaccatat tctctcttcg caatgtatta
     6121 tactttctct tcgtttttat ttttagaaat gctatctgat aaattaggta taaaaggaag
     6181 aggaaatttt cttgctgtat ttttgattgt cactcaagtt atatgtaatt tccctttagt
     6241 ttcttatcgc gtatctacat ttatgaaacg tgaatttcca aactcaggga aggcatggct
     6301 gataaatatc gggcagtttt ttatttgata tttattagca gtgtccctcc tagttatctt
     6361 tttattttat atacaaaaaa tccgtttcat tcatcgtttc atcgaatact tcgcttttga
     6421 ttcgattgaa caattaactc agcgttggaa aaaaagaatt cattggcttt ttgttcataa
     6481 aagttatttt ttagttgcag cattttttta ctgctttacc ttcgtgcaga ttttcgtttt
     6541 agaaccaaaa gaaggttgga aagaaactat ccaggttgcc ttaaaaatat ttactcagcg
     6601 acaagcaccc atgatcctga caatcatcat tattatggga tttttcttct tactattgtt
     6661 gatttccaat cgtttttggt acgccttgac tgctaccttg atcattaact tactgctcac
     6721 tatttcgaca gtaatcaaaa tggaaatgcg ggaagagcct gtctttcctt cagatttaaa
     6781 aatgctgacc ggattgtcag aactattatc gatggttagc ccagtcttat taatcgtggg
     6841 cggattgatc cttctcctat tattgattac tagtattatt gtacaacgta gactacaaaa
     6901 acagtattcc ttgaagattc attggaaacg tcgatttatc agtatcgctg tattattagg
     6961 atgtttttcg ggtgtctttt tcattaatca caaaaattcg ccttctttct tgctgtttaa
     7021 tttatttaaa gtaaacaaga ctttctttaa ccagcaaggc gccgtaaaag ataacggacc
     7081 agtcattcaa ttcctaaaca atatcgacat taaaatcatg gaagaacctg ctggctattc
     7141 gaaagaaaaa attaatggaa taatgaaaaa gtacaacaca aaggcagatg aaatcaacca
     7201 aacaagaagt gactggttaa acaatcagac gatcgttctc aatttaagtg aaagcttcag
     7261 tgatcctgca cgtgtcccta atctgacagt acctacagac cctattcctt acatcaaaag
     7321 cattataaca gaaggaacag gaggattgat gttatccgtc ggttatggtg gcggaacagc
     7381 taacatggaa tgggaaggac tgacaggctt gagtatcagt aacctgtctg cttcgttagt
     7441 tacaccttat acccagctag tggagcgtca gaaaataagt ccaaatatta ccgatctatt
     7501 cgatgaaaaa atagccatcc atccttttac agctgctctt tatcgcagaa aacaagtgtt
     7561 tgataaattt ggttttgaca ggttctatta tttagacagt ccggataaat taacttatac
     7621 ggataaaata gaacaaagcc cacgcatatc ggatgattca gcatacaaag aaaccttgaa
     7681 agctttacaa agcaatacgg aaacgtcaca attcatccaa ttatccacta tgcaaaacca
     7741 tatgccttat gatgattact atagtgaatt gaactacact gctgaaggaa ctgctgtcat
     7801 tcctaataga agaaaagaat tacaaactta tatgcaaggc cttcactata ccgatacagc
     7861 agtaaaaaaa ttcatccaag aaatcgacaa aatcgataaa ccgattacct ttgtttttta
     7921 tggtgaccat ttacccgcaa tctatagtgg gaatgattcg aaaaaatatg gattagaaca
     7981 gcaccagaca gattatttca tttataacaa caagtatagt cgtgaacaag cagctacgtt
     8041 aaataaagaa gttgttgctc ctaataactt tggtgctatg gctcttgagc atgcgaatat
     8101 caaagtaact ccttactatg cgttgttgac agaagtatct aatcatctac ctgcattaac
     8161 aattgatcct acagaaagct tgtctaatcg ttttaatgga aaacaggtct ttgttacaga
     8221 ggatagtcaa attatccaag aaaaaaattt aactgagaaa caaaaagaaa ttctagaaga
     8281 ttatcgtctg atacaatatg atcttgttgc aggtaaccag tacagtgcaa catgggcgac
     8341 acaaaaaata aaagactaac aaataaactg agcaaggggt tgtgataaaa gacatgtcac
     8401 aaccccttgt ttatctcatt aatttgcttg tccataaacg atttgattaa agccattttt
     8461 caattcatta ttcgctgtct cgttgaagta aagataatta tcgggattcc cctgattttg
     8521 ttgtgtcacc aatacttttg gctgattttg ctgaaacccg aatagataga cgtgcttttt
     8581 cagatatggt tctgtctctg catcggaata gacggctgtg atttgatagt ctgcttgacc
     8641 tgtcccactt tctgaccatt ctgcagaaac aggcactcca ccaatagcca tcttccactc
     8701 cccgttcaaa atagcttgag ggactttaag accatataag tccacactca tttgattcgt
     8761 ataacttttg tattgttgtc ccattgtttg gccccatttg gtcataaatg cctctaattg
     8821 tgcggatttg tctgcattcc acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga
     8881 agatgaagcc tctacagaag aacttgaagt agatgtttga gttgtttcag tcgttttgga
     8941 agactctgac gaagtagagg tatctgctgt ttctgtactt gctgcagttg acttgctctc
     9001 tgtgctagtt gttgtagtag ttgcaactgt tgctttcgct gtttcttttg ttgattcttt
     9061 tttcgattca ttcccgttgg tacaaccagc taacaaaaaa gctagaccaa ttgttactaa
     9121 aatccttttt ttcaattaaa aaactccttt cgattactcc cacttcttta ttctatcaaa
     9181 taagcaatgt gattttattg atttttcgta ttttctcatc aatctttcaa atcttctaaa
     9241 aactgagaag cacccgctga tgttgtctgt aatacatcag cgggtgcttc ttttatttta
     9301 atcttcgtga ttttccaatt tacctcacgc tttatatttg tatttgaaga aacatagact
     9361 tttagtcatc atcgtcttcc tcatccaaat cttgtcggac agtttttcct tggagtcttt
     9421 gtacttggta gatgatttct ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc
     9481 catatttttc ttctttttca gctaatttga ttgcccgtgt gataaagaca ttttggaaat
     9541 caaaatcttt tgcaaagttt tccaacattg ttgaggctgg taaatatttg ttttgaccat
     9601 gttcgtaaat ttttccataa cgcatgactt cttctaaagt agacggaacg atttcatttt
     9661 caatcactaa ctcatgagag attttattga accatctcgc ttcttgatca gcatattctg
     9721 aaaaaagggt acttaattct tgcgaagatg tacccaaaac atacaattgt gcttggatca
     9781 atttcatttc ttctaattta aaatttgcta atacatgccc caccatggcg ccagcagttg
     9841 gtttatgatg gtcgatatct gcttgttttg tttcttgaac aaatttttct tccactatat
     9901 tcattgattt acgcctcttt tacttttgct gattggactt cataacctaa tttttccaca
     9961 actgcagcta attgttcagg gtggacttca tctggatcaa agtttgcttt gattttgctc
    10021 gcattaaaca aaactttcgt attgcttacc ccaggctgtt gagaaaccgc atgttcgatt
    10081 ttttgcatac agcttggaca agctagtgta tctaattgca taataatttt tttcatcgta
    10141 gttcttcctt cctttttctt gatagtctta ttctagatta cagcttccat aaaaaaattg
    10201 attcttatca agttttacga tttttcttct ttgctttcgt gtccaaaata caataaacgc
    10261 atcgcgttaa agataacgat caagatacta gcttcgtgaa cgaacatccc actagccatg
    10321 taaataaacc cggcaaataa gccgttgagt aaaaataaga cgacaccaat ggcaatcact
    10381 acattttcac gggtatttaa aacagttttc ttagccaagc ggtaggcatg aaccaacgat
    10441 tcaaaattag attgcatcaa gacaacgtca ctcgtttcga tagcaacatc cgttcctgat
    10501 cccatagcaa tcccgatatc agccgcagcc aaagagggag aatcattgat tccatctcca
    10561 ataaaagcaa cgcgcaatcc ttgttcttgg tattttttca caaattgaac cttttcgtct
    10621 ggtaataatt cagcatggac ttcatcaatg cctaacatat cagctacata atcagcagtc
    10681 atctgattat cacctgtcag catcactaac tgttttgctc ctgcttgttt caacttagct
    10741 aattgttcag cgacttctgg tcgaatcaca tctgacacac ctatgatttg agtgattttt
    10801 ttgtcgattg ctacaataat gaccgagctg cctgtttgtt gtaattgatg taaatcttgt
    10861 acttgctttg gtgtgagaga aagtttgtga gcggcaatca atttggtgtt gcccgcacaa
    10921 actgtatgac catcaatctc agctgtgatt ccttgtccct tgattgtctg attatctttg
    10981 actgctaatt gttgaaaatc aatgtctttc tcctgagcat aatggatcac ggcgcgcccc
    11041 agaggatgat cggataacgt ttctaatcga gccactaaag ctaagctgtc cccttcatcc
    11101 tctgtatagt tcttgatagt ggatacagca gtagttcctt tggtcaatgt accagtttta
    11161 tcaaagacaa aagtatcgat tttggaaaaa gtatccattg cttcaccgcc tttgactaag
    11221 atgccccgtt tcgctccatt accgattcct gctacattcg aaacaggcgc accaatgact
    11281 aaagcgccag gacaaccaag aaccaaaaca gtgattgcta aacgaaaatc acgactaatc
    11341 agaccaacaa ccaacgcaat aagtaaaacg gttggagtat aatactgaga aaatcgatca
    11401 atgaattttt ccgcatggga tttggtatct tgtgcttctt ctactaattc gataattttg
    11461 gcaaaggtcg tatcatctcc gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa
    11521 atcgttccag agaagacttc atctccactg attttgacga cttgtcgtga ttcaccagtc
    11581 actgcggctt catccgcata ccctttcccg tcaatgactc gtccgtccac aggaatagct
    11641 ccaccagttt ttactaagac gatatcgcct tcttccacgt catcgatttc cacttcttca
    11701 gtcgttccat cttcttggac aacactagca gtagatgggg ccatctcggt caaagccttg
    11761 attgactccc gcgttttggt caatgttttt tgttccagat agctaccaaa caaaaacaag
    11821 aatgtaacga tagctgactc cttgtattct ccgatcataa aggcaccaag cacagcaata
    11881 gtaacgagta aatcaattga gataactttg actttcactg cctgatacgc atggatcata
    11941 ataggtatga ccgcaataat agaagctatg gctagagcaa tgttatagaa caagacaaaa
    12001 ccagtaaatt ttccgataaa accaaaaata atcaacacag ctgataaggc ggtgatatga
    12061 tttcgatgct cttggatcca cttttgtaat ttcatctttc tccctctttt cctttcattc
    12121 ttacgagact aagtatagat acttatccat cataaaaaat tgaccactat caagtttagt
    12181 caaaaaggcg gttttctttt ggtagaatac actacaaaaa atagttagca agaagactca
    12241 ctaaaagtga tcttgccaac tattgtcgtt tctatcgaaa ttttcattga atgattttcc
    12301 tgtacagcat acatcggaga tctatttctt tatctaagat ttttatcttg ctaatttttt
    12361 cctttagtct ttcaaatcca cgtcgttcat agaattgata cgtacagcca ctatctgtaa
    12421 aaaggaatat ttcttttcct ttctctcttt tttcaaattc tgttagcaac ttggtaccga
    12481 tgccttttgt tttcaaatca ggatttgcag ctaaaaaaat gatctctcca tctggcgaat
    12541 gcttctttaa atatgctgca agcatttgtt catttgctgt ctcgtaagct cctttacttt
    12601 ctttagcaaa tatattttgt aaaaaatcga atatcttaac atatatttgc tgtccaaaag
    12661 aacgatgttt ctttttttct ccttttatct ccgccagcaa aacacctgcc aactcgtcct
    12721 ctgcatacaa agcaagaatt tgagttgctc gattcaactc catatacaaa aaatatcttc
    12781 cgtaaagatt cagcagaagt tcattttcca tatatctctc aaagttcatt ccttcaacag
    12841 caaaatagat agcttttttg taatctttct tttctaaggg tttaaaagtt agtttcatca
    12901 tttcgcctcc tcaacttcaa tgattgtgtt atacttacat tataatagac acagtgtcta
    12961 ctatctataa tcaatcatct ataagtttgt cgattaagcg acagctttgc tttgatatgt
    13021 cggataagga gataggtaaa tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc
    13081 tttggctgaa acactatcca cattattagt aaataaaaag attcaagcaa tcactatacg
    13141 cgaactaaca gaaacggcag atgtacatcg ttcgactttt tatacacatt ataaggatat
    13201 ctatgatcta tatgatcaat tagaaagcaa cttttttcgg gatctaaatg aaattctctc
    13261 ttatgatcca gcccatagct atgaggaact atacactcgc ttgattgact atgtttacgc
    13321 aaatactttt ttatatcgct tatttatcct caaccatggt gatatttcgt ttcagaagaa
    13381 agttaaacaa gttattgaag aaaactatct gaacatatgg ctatatgaag acaagaaaac
    13441 gattatcaca gaagaaatga gattctttac tacgtaccac gtccaaggtt gtctatccgt
    13501 tctcaatcgg tgggtagaaa gcaattacaa gtactccaaa aaagaattat tagaactctt
    13561 acagaaactg aatgatcaca tagagaaaat catgccttga ataaaaacat actaagctta
    13621 gttaagaaga accgtaacct tctcgtataa caactgaatc ttatttagcc aaattgttta
    13681 ctgaagattt tttgagtaaa atttctataa ttcctactta ttgaaaagta gtttgtagtg
    13741 ctgcttctcc ctctctaagt gatatatctt gttgtaccaa tcaaattcac ctcatcatta
    13801 ataaattact tttaggctaa tttattacaa cttataggtt gaagctgtaa ccaatttata
    13861 gtcatattat atttttatca tccagtttat tgaattcttc tcgtttacct caattcattt
    13921 tcaaggggaa tcagctcatc atctaacatt ttacgccatg ctcgcttcat ttcttgtggg
    13981 tatttaaatg gtttcgcttc tggatgtttg gctttatatc cttcgtaatc cttactgttg
    14041 ataattgctt tcatattagg aattggatct tcacctggca tatattgcag attagaagca
    14101 tgtaattttt tctcagaaac aaaccactca ttggaaaaga catgaatcaa atcatcactt
    14161 acttgtgtaa aatagcgacg tttaatgaca aagatatctt cattgctatc tagtgtacct
    14221 tcatttcttt gtgcttaaat tcaaagataa ttttctcaat aaagattttt gattccagta
    14281 agattccttt actcctttta caacatagcg ttgtcctttg ttttgaaaca tcttcttccc
    14341 ctctttgcca ttaaaaatag cccaattctc tataaagaag attaagcttc attattataa
    14401 tataaagttt aaatttggtt tattacttac tttttcttat tctctagtaa gctaatattg
    14461 aaaaaaatgt aatcagatca ttatttttat atgttgtaac ttcctgataa gaaaactaat
    14521 taaaagaaag aggtaactac ataaactaat taaaaaagct aacttcatag ttggaggata
    14581 aatggaagaa acttcaatga ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat
    14641 tatcaatctc tcttttttgt ccatttactt ttgcgactgt tccccaataa tcaaatacag
    14701 gaagcgttat tttttgtcct ttagattcat gtactgtata gactattttc gaatccatca
    14761 cttgataagg catagtatgc cattccttgc caattcttac ttcatgattc tgcaaatgtt
    14821 ctttgtattg taatgcttcc gtgggtgcat agtcataaag tcctccggga aggagtgtcg
    14881 tgtaacgaat aagattttct tgattcatta ccaagtgatc tctctgagtg acaactttat
    14941 gttcatagtt tgcttttgtt gcagcagata cagccaacag taataaaaca attgccgtta
    15001 tattaactac ttttctttgc ttagctgatg tgctcttcat gaaaacgaac gataaaatca
    15061 acgagccaaa cagtacttga aagcctaaga ttcgccaagg aaattgaatc atttgaatgg
    15121 gtgtattttg taataaagac catggaaaaa atttggtgga taagagccaa gagactaccg
    15181 cactaaggta aatcactttt tctttgcgat taaatccttt aaaaaagaag agtggacaga
    15241 tcaaagaaat aataccaatg attcctaccg taaaggttgt caggtcatta ttcatcgcac
    15301 caatcaaact atccgagaca tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat
    15361 taatcgattg aaccatgatt tgtcggaaca tcggatacca aaagtatgct gttaaaagaa
    15421 tcgtcattcc tactgccttt gacaactcca caatcaaccg gatgctttct ttttttcttg
    15481 tttcacttac tacaaagccg cctaagacta gtaagccaac ggctattaca ctcataaaag
    15541 tggaaagaac atgtgtataa atgagtaagc tcatactgac tgctaatgga attgcacatg
    15601 gctttttctc ataaatcagt gcgtaagcat aatacataac tatcggtata accgtaagtg
    15661 caatcgcttc agcaatagca gaacgatgat aaatatcaat cgtacggtac ccataaaatg
    15721 tatataaaca gctaaatata aaagccgcat cgattctttt taaaaatttt agtccataat
    15781 gaaacatcag catgatagta caaacattca atagccaaac atataagata taactaacga
    15841 ttaaattatt acttatccaa taaaaaatga ctgccggaaa aaaggtcaag tagggataaa
    15901 aataattcac tccgctccca taatgattaa acgtcgtaaa attgattgga ccagaaaaaa
    15961 ttgaagataa ccctttgact ctagcaaaat ggaaggcaat atcccccgat gtagaaatag
    16021 tatgaacatt tctaaaataa ttgattgaat aaagtccagc aataattgct aggagaatgg
    16081 acaatattat ttttttcttt acttgcatta cttaattata tactcctttc atggattgct
    16141 ttatctagta taacttaaat tttaaataaa tcgttattta tttttactct cacttaaaaa
    16201 agcactaaaa ttataagaga gggaggtaat ggtctgaaca ccaagaattg ttccaacatc
    16261 gaattgctta actaatgagt tcaaagctta attatgtggc tcttatattt accgagcgtg
    16321 gattgcgatc tctttagcga aacaaacatc tttcataaaa aaaacagcca tatccgcttt
    16381 cttggatatg actatttact actactttac tatctttcat tcttcttgtc tcacttatga
    16441 taaggcgctg ggtatcatga gtgaatgata ttgctgaata caaatagtcc gaccacatta
    16501 agttgatcgg acttcataag cattttattg actaatactt ttttgcttaa gaattgattc
    16561 taacatcttt tgctaaagtt aagcatccta tagttcctgc attatctgct aacttaggtg
    16621 tgacaatgta ttcttcgata tttggcgtat cgacatagcc gttaattagt tctaaaaaac
    16681 gtctttgtac tttctttttc agatgttctt gttgcatgac accaccacca aaaattataa
    16741 tatctggtga aagcatcagg gtcgtattac aggcacattg tgcaatataa tccgcctcga
    16801 tttcccagaa cggatcttct atagaaagat cttgtccctt ggttcccgta cgcatctcga
    16861 tagcgggtcc agatgccaac ccttcgagac aatcatgatg aaaaggacaa gtaccagaga
    16921 aagtatctcc tgaatgtctc ttaactaacg tatgccccat ttctggatgg ctaaaccctt
    16981 caacaaaaat cccatcttgt atagctcctc caccaattcc agttccaaca gtgtaataaa
    17041 caacactgga taaacctttt ccatgacctg aaacatactc cccgtaacaa gcagcattca
    17101 catcggttgt ccaggcaatt ggaatttcga aatgcttatt caacgttcca acaaaatcaa
    17161 aatttttcca ggcagttttt ggagtagagg tgatatgtcc ataggtcttt gattcacgat
    17221 ggatatcaat cggtccaaat gaccctactc caatgctttc taattgttct ttatagtgat
    17281 taaagaaatc aattaccaaa ctcattgttt cattcggtgt agttgtagga aaactcactc
    17341 tttctaaaac ctttaaatca tcattgccaa tggcacaaac aaatttagtc ccacctgctt
    17401 ctattgagcc atacatttct tactccaccg tccttttatc taggaattcg ttcttccctg
    17461 tctaatagtt tttcgaaaaa ctcatgtggt tccttttggt accaataggc aacagaagaa
    17521 atatcgtctt gacgctcata aagcttgatg tcatcatttc ctagggtttg gaaagtaaca
    17581 cgtaaatttt ctttgaaact aatggggtcg ggtaaatgcc agcggtacaa cccatgcatc
    17641 ggtaaagcat catcgccaaa accatgaact ggatttggat taggttttcc agcactaaaa
    17701 taatcccggg tgtgatctct tttagattga aaaggatagc ccataaatag cgtattaaaa
    17761 ttttgagcat atgccctttc tccaaagttc tttttatgga aagcccaagc gccgccaaaa
    17821 taatcttctg ctcctgtaga tgttattgta gggaaatctt catctccatc tatataaaac
    17881 ttaaattctc cttcccccca ccagtatctt tccaatgcgc acaaagcaaa gtaggttcct
    17941 acataatacc cagtgccttt gattccatca ataagggtgt aatcttttcc ttttgtcgtg
    18001 atgggttcac ggttccattt agcatgaaaa tacatcgcat catccggtac ttcagtcaat
    18061 gcataattaa atgtataaaa aatgtgctta ataaactcag gatgttcatt cgttaaagta
    18121 atcttagctt tttttcgaaa tggcatctca aaatagcaat tcatccctga tgttggattc
    18181 actacgattg gtaaggaatt cacttcgcat cttgtcccaa acccattaca aaaaaaatct
    18241 cctaatggac tttctacaga aggagtggat tcatcatccc aataaatacg caaaattaaa
    18301 tcacgtaaga cgaaactccc agcgattgtt ttgtctgtca ctgtcatcca aaaatgacgt
    18361 aaaattccag ggccttccat ttctgctaaa gtaattgtct ctccagcatt tatcggaaca
    18421 gaaggtgacc cttttctccc aattcctaga tgactcgctg tcattgccgc tttccctttt
    18481 tcaccatttt tattttctgg tgaaatagtt ctactttcct ctcccttgtg gagccatata
    18541 ttatttaaac aattcatttt attcgtcctc tatcctttct tcactttttt gattattcat
    18601 atagatgatt tgttgttgtg cctccgcaat aattgctcca atatctaaat tatcatttcc
    18661 taactgcaaa gcgattgaca ttgccaaggg aagattcatt cctgcaatta cgtgcactct
    18721 gtcagaaata taggtaaaaa acttttgatt tacacttcct ccagccatgt cagttaaaac
    18781 aattatttcg tctgtagtat ccactaactc tttaatttgt tcatctatat catcaatttc
    18841 ttcgacatat gcacataagc tactaatttg attatgcata cctgttaaaa actctagtgt
    18901 ctctttaaaa ccctcagcta gtattgaatg gctagcaatg atcaatcttc tcataattca
    18961 aatccttttc ctttacgcta atattccaaa tgcagccata accatcgcaa aaacaataac
    19021 gagtagaatc aaaatggtca tactgatttt tttcttactg agtagataat atgctgcagc
    19081 agtaataatt actggaatta atgttggtaa aattttgtcc aacattggtt ggattttcat
    19141 actaacttca ccaaaagtga atttaagtgg cgttgaaaca ctgataactg aagcaattaa
    19201 tgatccaact actgttagcc ccaatactga agcagcttca gttaaataat taatcttttc
    19261 accgtattca gtaacaatct tcattccctg cttataacca aattcaaagc ctctcattcg
    19321 gaaaaagaag attaccatga ttaaaatgaa ccataaaaag attcctacag gattattttc
    19381 taaagccata taagccgcaa ttgatcccat gatggttggg atcatcgtca tcaaaagaga
    19441 gtccccaata ccagcaattg gacccatcgt accaacttta aagtcattaa tcgcatcttt
    19501 tgcttctaat ccttgctttt cctccattgc aacacaagca cctaaaataa tggctgccag
    19561 ccaaggctgt gtattgtaat actgaaagtg attatttaaa gcttcgatgt attcatcatc
    19621 atctttgtat aattttctta aaataggtga caatgcgtaa gtcacagatg caccttgctg
    19681 tgtttgataa ttaaaggtac aaactgacat caaccaacga tagttcgctt tcctaagatc
    19741 ctttttcgtt acttgatatt tttcgctcat tttattcatc ctccccaata aacccaacat
    19801 cagaagctcc cgcagaagca gtgatttgtg catctttttc agctctatga tacacattaa
    19861 atgctaaagc gaatcctatg atcgccgctc caataatagg taccttcaaa aaagcggcta
    19921 agaaaaatcc aacaagtaaa taactaatgt atcgacctgt aggcataaag cgtaacaaca
    19981 tcgcaatacc aacagcaggc aacatttttc cggctaatgt tagcccatcc ataaaccaat
    20041 taggtaaaac atccaaaacg taattaacag cagactgtcc taaagtaacg gcaataaata
    20101 ctggaattgc tgcagataaa ccaaagaaaa ttggccccaa ccacaaaaca ctattcattt
    20161 tcttaaattc tcgtttattc gcatattcct gtgatttgcg aacaacaaaa ccatttaaga
    20221 ttttgacaat tacatctagt tgcaccccta acatacctac aggtagtcct attgctaatc
    20281 cggcttctac tccaagctgg ctaccaaatg ccgttgcaat aattgcgcac gttccatagt
    20341 ccggtacaga tgagccacca aaaccggcaa ctcctaatga catcaattga actgtaccgc
    20401 caactatcaa acctgttttt atatcaccca tcaaaattcc tgtgaataat ccccaaaaaa
    20461 ctgcgtaatt tacaaagatc atcggaatcc cataataatc aacatgttta ataaatgcta
    20521 atatagtcag cataataatt tgaaaaatag taaaatgcat tatccttact cctttatcct
    20581 aattttgatt gaatttcctg tgccttatcc gccggaacaa attgtgtaac aatttctaca
    20641 ccagaatcca acaatttttt atacgtgctg atattttcat ctgatgcata ggctctagaa
    20701 gatagctgta atgcattatc tttaacaaca gtaccgccaa caattagtcg gtcaattcta
    20761 ataccgttat ctactaaatg aaggattgtt tccggctctt tcacgatgac aaatactttt
    20821 tcctgatcgt atttcccctt tttaaaattc gcaatcgctg tttcttcagt gataattgat
    20881 acagccattc cagcaggtct agctaaacgc atacttgatt tttggatgtc gttatttgcg
    20941 acagaatcat cgatcaccat cactctgttt gctttagaca ttggtgccca ttgcgtgaca
    21001 ataatcccat gcaataaacg gttatcaact ctcgacataa caatactcat aaatacttcc
    21061 tcctttatct ttcattttct tacaagcaat taccatgcca aaacaaaaag aacttgataa
    21121 atcaagctct ttttacataa tcataaacgt atttaacctc atgaatcggg atgtcgacac
    21181 tataataatg ttcaacgaca ctgaaagatt gcttaaaatg tgtctgaaat ttttgaaatt
    21241 cctcactcgt ttcattgaaa taaatttcat cttccaaagt attttgtttt actaatcgtt
    21301 caattaagca actaatgtga acatataatc cgaaacacgt attattatca aatgaaattc
    21361 ctagatcatt ttgtaagatg tatatagcat ctgaaacccg ttctaataat ttagtcggat
    21421 ttaatatggt caattgctcc attaaattcg ttagactaaa attcttcaga atattattgt
    21481 tcaaaacttt cagttcatta tcattaaagt gtctattcaa ttgattggag aattttctta
    21541 tattcattcc taaaatcata tcttcaattg aaaagaattc tacatcttct agatgtggat
    21601 ttagtgttcc aacaataaaa agaatattgt tctctttacg aagctccgat aaatagtttt
    21661 cattcactaa agagtaatag tcacacgttt ctatgacaag atcagtatcc cttggaaagc
    21721 tatcttcaag tatccttttt aatttaaacg aagttcctaa tccgcttgca caagaacaga
    21781 caatcatatc cttcttaata tttgatgatt cataaatata atccacattt tgattttctt
    21841 ttacttgttc aagaatctgt ttaagcggta aatctaataa tatctgattt gcaacatcaa
    21901 tagccattct tgttgaaaca ttgtttagta gtccgaattt tttttgagcg tctagttcca
    21961 aatttagata aattttctcc agtgaaccca tatcaacaag taaaataatt tcttcatatc
    22021 cggtacactc ttgtaagtat ctattaattt tgtctgcaat ctcactactt gaagtcatta
    22081 gtggcatatc aatggcgtcg tatatatatc tctcaacgat cctattagca gtacttgcta
    22141 tacaggttgc cgttagatct ccatgagcca aaatcagtgc taaacgtttt tgctgaaatt
    22201 ctttactaca cattaactta aataaaataa ccaaaacatt tttttgaatt tttgttaaat
    22261 aaatatcccc tatttgaata aaacgattga ctaattgaac ttct
//
LOCUS       DAYYNQ010000047        22169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000047 DAYYNQ010000000
VERSION     DAYYNQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.047"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            234..2069
                     /gene="tdc"
                     /locus_tag="ACHBJ1_001626"
     CDS             234..2069
                     /gene="tdc"
                     /locus_tag="ACHBJ1_001626"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM9430563.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2374..3852
                     /locus_tag="ACHBJ1_001627"
     CDS             2374..3852
                     /locus_tag="ACHBJ1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9430564.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3818..5149
                     /locus_tag="ACHBJ1_001628"
     CDS             3818..5149
                     /locus_tag="ACHBJ1_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9430565.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5263..7815
                     /locus_tag="ACHBJ1_001629"
     CDS             5263..7815
                     /locus_tag="ACHBJ1_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM9430566.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7822..9261)
                     /locus_tag="ACHBJ1_001630"
     CDS             complement(7822..9261)
                     /locus_tag="ACHBJ1_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430567.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9236..9418
                     /locus_tag="ACHBJ1_001631"
     CDS             9236..9418
                     /locus_tag="ACHBJ1_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430568.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9607..9969
                     /locus_tag="ACHBJ1_001632"
     CDS             9607..9969
                     /locus_tag="ACHBJ1_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM9430569.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10156..11013)
                     /locus_tag="ACHBJ1_001633"
     CDS             complement(10156..11013)
                     /locus_tag="ACHBJ1_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9430570.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11133..12044
                     /locus_tag="ACHBJ1_001634"
     CDS             11133..12044
                     /locus_tag="ACHBJ1_001634"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM9430571.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12051..13702
                     /gene="mdcA"
                     /locus_tag="ACHBJ1_001635"
                     /pseudo
     CDS             12051..13702
                     /gene="mdcA"
                     /locus_tag="ACHBJ1_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13804..14103
                     /locus_tag="ACHBJ1_001636"
     CDS             13804..14103
                     /locus_tag="ACHBJ1_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM9430572.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14093..15769
                     /gene="mdcD"
                     /locus_tag="ACHBJ1_001637"
     CDS             14093..15769
                     /gene="mdcD"
                     /locus_tag="ACHBJ1_001637"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM9430573.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15773..16366
                     /locus_tag="ACHBJ1_001638"
     CDS             15773..16366
                     /locus_tag="ACHBJ1_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM9430574.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16390..17466
                     /locus_tag="ACHBJ1_001639"
     CDS             16390..17466
                     /locus_tag="ACHBJ1_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9430575.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17491..18360
                     /gene="mdcB"
                     /locus_tag="ACHBJ1_001640"
     CDS             17491..18360
                     /gene="mdcB"
                     /locus_tag="ACHBJ1_001640"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM9430576.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18471..19235
                     /locus_tag="ACHBJ1_001641"
     CDS             18471..19235
                     /locus_tag="ACHBJ1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM9430577.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19724..21817
                     /locus_tag="ACHBJ1_001642"
     CDS             19724..21817
                     /locus_tag="ACHBJ1_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9430578.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21842..22144
                     /locus_tag="ACHBJ1_001643"
     CDS             21842..22144
                     /locus_tag="ACHBJ1_001643"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9430579.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4723 g         6479 t
ORIGIN      
        1 ttcgcttttt ttaggttatc tattttagaa gactgattta ttgaaaaatt gttcgctttt
       61 ttgcgttgaa ttatcagaaa aatccgactt tatttatttt tgtttgtaaa aatctacgtg
      121 gatttgatgt tataaaaaaa aaattctcat ttttggcgct atagtgaaga ggtgttacag
      181 ataaacacat gtatctgcaa gacattgacc aacaaaatat ggagggaaga aaaatgaaag
      241 atatggatat caaggccgtc tttataggcg acaaagctga aaatggacca gtttataaaa
      301 tgttgttgaa caagatggta gatgaacatc ttggatggag agaaaattat attccttcag
      361 atatgcctgc aattagcgaa ggagataagc tcactccaga ttatctagcc acccgcgacc
      421 atatgataga ggtcttggat gaagtcagtc agcgattacg cgcaggatcg atcccatggc
      481 attcagcagg ccgttactgg ggacaaatga atgctgaaac attgatgcct gctttattag
      541 cttacaacta tgcgatgctg tggaacccaa ataatgttgc attggaatct tctatggcaa
      601 cttcgcaaat ggaagcagaa gtaggacagg attttgcttc attattcaat atgacagacg
      661 gatggggaca cattgcagca gatggttcta ttgctaatct tgaaggatta tggtacgccc
      721 gttgcatcaa atcgattcct ttagctgtca aggaagtgct gcctgaaaaa gtgaaaaaaa
      781 tgtctgagtg ggaacttttg aatttatctg ttgaagaaat tttggaaatg accgaaagct
      841 ttacggacga agaaatggat gaggtcaaag cagcttcttc ccgtagcgga aaaaatatcc
      901 aaagattagg gaaatggctg gtccctcaaa caaaacacta ttcttggatg aaggcgttag
      961 atatttgcgg agtcggctta gatcagatgg ttgctatccc agttcaagag gattatcgaa
     1021 tggatattaa tgctttggaa aaaacaattc gtgaattagc tggacaaaaa atcccaattc
     1081 taggtgtggt tgctgtcgta ggaacgacag aagaaggtca agtagacagt gtagataaaa
     1141 tcgtccaatt aagagaaagg ttaaaagatg aagggatcta tttctattta catgttgatg
     1201 ccgcatatgg cggctatgcg cgttcactgt ttctaaacga agcaggagaa tttgtgccgt
     1261 atgcttcctt agctgaattc tttgaagaac atcatgtttt ccaccactgc gtaacgattg
     1321 acaaagaggt atatgaaggg ttcagagcta tttcagaagc ggattctgtc acgatagatc
     1381 ctcataagat gggctatgtc ccttatgctg ctggaggaat cgtaatcaaa cataaaaata
     1441 tgagaaatat catttcttat ttcgcgccat atgtgtttga aaaatcagta aaggctccag
     1501 atatgttagg agcttatatt ttggaaggat caaaagctgg agcaaccgcc gccgcagtct
     1561 ggacagcaca tcgtgtattg ccattgaatg tgaccggata tggtcaattg attggggctt
     1621 caatcgaggc agctcaaaga ttcagagaat ttctcgatca tctgactttt actgttaaag
     1681 gaaaaacaat cgaagtctat ccattgaatc atcctgattt taatatggtc aattgggtat
     1741 tcaaagaaca aggctgtaca gatttgaacg ctatcaatga attaaatgaa aaaatgttcg
     1801 atcggtcttc ttatatggat ggcgatgttt atggtgaacg gtttattact tctcatacta
     1861 catttacaca agaagattac ggtgattctc cgattcgttt tgttgaaaga atgggtctaa
     1921 cgaaagaaga atggaaaaaa gaacagaaaa tcactctgct acgtgcggct atcatgactc
     1981 cttatttgaa tgatgatcga attttcaatt tttatacaaa aaaaatcgcc aaagcaatgg
     2041 aaaagaaact aaacgaaatc atccaataga actgattttt gtgtgaaaga ataacaagag
     2101 acatatggag aacagacaat tatttgcttc atcaaaacaa ttgtatttcc ccctgttttt
     2161 catatattcc tcgccaagat attctttttt ataaaacgtt ctataagttg gatcgttcgt
     2221 ttcatgtatc aaacccagtt cactaagatc gttcaatgga tgtttcggtg atactgggtt
     2281 ttgtactgaa tcgatgaaac cttgtcattt ctgttcttga acaaaaataa aagaaaacaa
     2341 aaacatatgt cgctattgaa agaaggaata aagatgaaag atactggttc atcaaagcaa
     2401 atcacgacca tgacctttat cggaatgacc tgcgcccttg tagcaagtgt acgaaatatt
     2461 ccagatgtag cagcaacgag ctggacgatg attttttaca tgctggttgc agtattattg
     2521 tacgcttttc caatctcatt gatctctggt gaatttgctg gaatgtttcc ccaaaaaggt
     2581 ggtccagaac tttgggtatc gaatgctcta ggaaaaaaat gggggttcgt tgtctcatgg
     2641 ctgttatggg tccaaatgtt tcctgggatg gtgatggtcg cttcagcact cgctccgttg
     2701 tttggtaata ttatcgataa tgtgccgctt ggtttgaaca gcaaatttac attagttgtc
     2761 attttagtcg tttattggat catcactttt ttgaatttga aatttgatat ggcaaaaatc
     2821 ggcggaaaag tcggtgtttg gttaggattg tacgtgcctt tgacgatgat gctcttatta
     2881 gggtttgctg cgtggatcaa agttgggatc gtaccaaccg gaacattagg cagtttttca
     2941 tgggacaaat taataccaga tactacgact gcatcctcat ttgtttattt tgcgccgatc
     3001 atgtttattt ttactggtat cgaaatgtcc tctgtgtata tcactcgatt ggaaaatcca
     3061 gttaaaacgt atattcgcgg agtatttgcc gcgttaattt ttatattttt cgtcaatatt
     3121 ttgaatgctt tagttgtagc aaatgtcgta ccaaaaggac aaatggaatt gaataatatc
     3181 gcgcaatcta tttcgattta ttgccaaatt cttggtttgc cacatcttat tgtgaatctg
     3241 tttagtttac ttgtcttcat tggtgtagca gtccaattat ctgcttgggc ctcaggacca
     3301 gcaaagacag tgacagaaag tgctagaaaa ggtgcgtatc caccaaaatt caatttttgg
     3361 aagacgaatc aatttgacgt atccaaatcc gttattttga ctcagtctgt cattatctca
     3421 atctttgcac ttttttattt gctgattcct ggcgtcaatc aagcattttt gatgctcgta
     3481 aattcaacta ctgttatcta ctgtatcgtc tatgtcatca tggggatcgc agttttgaga
     3541 ttgcgttata cacatgcaaa gttgaatcga ccatttagaa ttggaaaaaa agagaaaaag
     3601 agcaatcttg gagtttggac tgtcgttatt gttttatttg cggcaatcgg attttctgtc
     3661 ggactgacga tgaaagctgg aacatggatc aatctaattg cagtcacagc catcagtgtc
     3721 attttgtttg tagtaccact ctgtattgaa aaaataaaga aaccttcttg ggaacaggaa
     3781 gtcgccatgc tgcttgaaaa agaaaaaggt gaacttgatg gaaaaaccgg caatgaaacg
     3841 gactctaact aatcgaaata tccagttaat cgcattaggt ggcgcaattg gaacaggatt
     3901 atttttaggt tcagcaaagg caattgcatc agcagggcca gctgttattc tcgtttattt
     3961 gatatgcggt ttggctattt atggtttgat gcgggccatg ggtgatttat ttttagcaaa
     4021 tagtacattt aactctatca gtgatttcgt tgactattat ttaggcaaaa agtgggcatt
     4081 ttttgtcaat tggacttatt ggctttgctg gcttggagct ggacttgcgg aattaacagc
     4141 aataggattg tatatccatt tctggtttgt acatgtacca gctttgataa gcggaggtag
     4201 cgcactaatg atcttaatgg cagtcaacct tgttgcagtc aagtggtttg gtgaattgga
     4261 atcttttttc tcaatcatca agatcttggg gattttcctt tttatcttat tgggtatagg
     4321 attgagtgtc tcagattttc atacccagtc tatcggaatc aaagaaaatt ttggacagat
     4381 cgatgaatta ctaccgtttg gctttgctgg aatggtagtc tcatttccta tggttttatt
     4441 cagttttgct ggaattgaaa tgatcggctt aaccgttggc gagacaagtg atccgcaaaa
     4501 gaatttgaaa aaagcaatca actgtctgcc ttggcgtatt ctctttttct atataggaac
     4561 gattttgtcg attttatttg ttgttccatg gcattgttta gatttaaaag aaagtccttt
     4621 agtgactatg ctgcaagtga tccattgcca aggcggagca gcaattgtca atctaatgat
     4681 tttggttgct tctttgtcct cagcaaatag tgcagtctac agtactagca ggattttata
     4741 tcaaacagca aaagaaaaaa gtttgcccca ttggttttgt gcgctttcta agaaaggcgt
     4801 acctttacac ggtattttaa ttacaggaat gttatttttt gccggtcttt gtctacattt
     4861 ttttgtagct gatagtcgat tgttttttca gcttttggca ggcatgacaa cgagttgttt
     4921 tctatttgtg tggtcttcga tcttgtgtgc acatgttcaa tttattaaag aaaagaaacg
     4981 gaacactaca caaagatata aagatcttgc tttgttggtg ttttttactt caattgcttt
     5041 cagcttgtta ttcgaaagca cgacacgctt tattcctttt ttattggtta tctggtttgt
     5101 attcttaaat tttgtatata aaagaataag caatagaaat atcagttagg cccagataca
     5161 ttttgcaaaa cgaattattt cagacccccc tgaaataatc gatacgcctg aaaaaacttc
     5221 cctgcgtata ataacagtat caattaaaca ggaggggata ggatgaactg gtacagattg
     5281 acggtagaag atgtattgaa aaactatcaa acatcaacag aaggcttgtc gcaagaagaa
     5341 cgacagaaac gcctgactga tcaaggatat aatgaaataa aagcaaggca gcagacaaaa
     5401 cgatggaaaa aaatagcaaa gcattttact gatctgctga tgatcgtgtt gatcattgcc
     5461 tcttttttga aatttgcttc tagtgaatat attgaaggaa gtattatttt attcgtagtg
     5521 atcgtcaatg gactcgttag ttactggcaa gaaagaaaag cggaagaatc attgaatggt
     5581 ttgaagcagc tgatgggaca agaagcgata gtactatcaa acgggatgca agaaaaaatc
     5641 cctgcgaaag aactggtaaa aggagatgtc attgtcttac gtgccggaga tgtcgttcca
     5701 gcagatatcc gattaatcga agcacatgat ttattagtgg aagaatcgat tttgacggga
     5761 gaatcagaag catctgaaaa aagtcatgcc gttttgacag aagaagaatc gatcggcgac
     5821 cagaaaaata tgggtttttc aggaacgctt gtacaatctg gttctgcagt cggtgtcgtt
     5881 gtagaaacag gaaacacgac agaaatcggt aaaatcaatc aagcattgca atcggtcgaa
     5941 caacaaacga cgcctctgat taaaaaaatc aaccgattaa acaaacaaat ttttcaaggc
     6001 attctttgtt tgagcctatt cttagttatt tttacgacat ttcgatatgg tatggattgg
     6061 catatccttc tttcggctac gattgctttg gtggtgtcaa tggtacccga aggattgcca
     6121 gctgttttga ccatgattct atccgttgga gtacatgaaa tggcaaaaga aaaggcgatc
     6181 atcaaaggac ttccttcggt agaaacatta ggttctatga cagtgatctg ttcagataag
     6241 acaggaacat tgacaaaaaa tgaaatgaca gttgtggatg tagctgcaaa agaagaagcc
     6301 tgcgtgttgt ccattatgaa aaactgtcag gaattaaaaa caaaagaaga acagaaaaca
     6361 gaaaatttaa gtggcaatcc gacagaagtt gcgctgattc gacacacaga aacagccaat
     6421 ctacctttga aaaaaataga agcaaagatt ccgttcagtt ctgaatacaa atatatggca
     6481 acgatgcatg cggaggaaaa aggagcggtg atttacgtca aaggtgcacc agaagtatta
     6541 tttgctaaat caactctttc ttctgcagaa caagaagcat ggagtcagac agctgcagaa
     6601 tttgcgagaa aaggtcagcg tgttttagga tttgcttata aaaaagtaga ttcgaaacag
     6661 gaactcacgc atgagacttt aacccaatta acttttgcag ggatcgccgg attgatcgat
     6721 ccgccaaaag aaagtgcagt caaagctgta aaggaatgcc aacaagcagg gatctcagtg
     6781 aaaatgatta caggggatca caaagacaca gcaaaagcga ttgcggaaca aattggctta
     6841 aaacatactg ccaaagtttt agaagggatc gatttggatc ttatgtctga tgaagagttg
     6901 attcagcaag tgccaatagt cgatgtcttt gctcgaacga caccagaaca taaactgcga
     6961 attgttaaag ctttgcagaa aaatggagaa atcgtcggaa tgaccggaga cggcgtaaat
     7021 gatgcacctg ctttgaaaag atccgatgta ggaattgcta tgggaatcaa aggtagtgaa
     7081 gtaagcaaac aggcagctga tatggtttta ggagatgata atttccatac gattgcaaaa
     7141 gccgtaaaag agggccggcg gattttggat aatttgcaaa aaaccatcaa tttcttcttg
     7201 ccaactgcat tagctcaagg attgatcttg atttgggcac ttatgctgaa tcgcccgcta
     7261 cctttgtccc cagtacagat tttatgggtc aatatggtaa cgacaatcac tttatcttat
     7321 gcactagggt tcgagtcgcc agatccagaa atcatgaagc gcccaccaag agatccaaag
     7381 caaggaatcc tatctggtta tcatttgttt cggatcatct atgtctccct actgatcatg
     7441 gttcctgcct atttattggc tatgcagttt gaagggcaaa gcttacagca aaccattctt
     7501 ctgcaaaata tcgtcttagc tcaagctgtt tacatgatca attgcagaga gttgtccaag
     7561 ccgtcattga accaaggtct tttgcaaaac aaagcgcttt ttctttcatt aggtatcctt
     7621 gctttattgc aagggatggt tatttttcta ccagtcggcc agcagctgat cggtacagta
     7681 agtttaacga tgacacaaca gttattgatt ggagcaaatg tgattcttct attcttagtt
     7741 gtagaaatag aaaaagggat catgaacaaa ctgtttcgca aaaaagaaaa aacagctaga
     7801 gaatacagtc gctagccgtt actataaaaa agttttgaaa gtgagttcag tcaaacgatt
     7861 gagctcattt ttcgttggaa tagaagaaat gtatacaata gggatatcta gagaatctag
     7921 agggaaattg gaaatcaaga tatctgtttt ttcaaaagaa atagtggaaa gatcggttag
     7981 ttcgataggt aagaagcaga cacgttttcc taagtaatct tttaattcct gctgcaagaa
     8041 cgccttccat gaaggttctc cttggaaaat aaaatagaca ttgaacaaat tcggtttgat
     8101 agaaaaaatc gtttgttgga tcagtagaga caagagttgg agaaagtaat ttggttcatt
     8161 gatttctatt tcttttttta gatcgttcag taaattctct ttctgttgat acaagtgtgc
     8221 atattgctga tcatatgttt ctaaaaaacg atggtattga cggtccaatg cttcaatcaa
     8281 tatcggcgat tcatgatatt ttaatatgag gagagccaag ttatcagtca gagatgtttt
     8341 ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt cgaggacctc
     8401 taaaaatgga gaatgatgca ggcagcgagc ggtttcttgt ccatagttat tgttgtaatt
     8461 ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttaa gataaatacc
     8521 ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg tgctgttggt
     8581 atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga taaagaacca
     8641 acatgttccg aaaatctctt ctgtttcaac cgctagttca gtttgactga ggtttcttaa
     8701 aaattgaaaa taatgggatt catggatcga ataatcctcg aaaaaatagt tgttatgagt
     8761 gaaaggaatc agcaatcgat gataaaaagt acgaatcgca cgctcttttc caagaataca
     8821 agctgtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct gattgatttt
     8881 agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa aatcagggac
     8941 agcaactttt tttgtaaaaa gcagattttt cagcagttcg acactgactg attgtttgaa
     9001 aaaaatctcc caaaggtcat ttgttaataa attttgctgg ttttgcaaac tgattcctgc
     9061 ttgatcgctg atgatgtccc agccttcggg cagctgagag cgaatagatt gaaggtcaga
     9121 aaaaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg ttatctttgt
     9181 atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt cggtaatgat
     9241 ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata cggagaaaat
     9301 aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg gagcatttac
     9361 tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca aaaaataggt
     9421 gggcaaacaa taataaacat tttaatagta gatggtagca agaccaacac aggcggattg
     9481 aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca actacttgta
     9541 ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag aaagagatgg
     9601 aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata caatttgtct
     9661 ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca aaaaagcaaa
     9721 aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga tgcaagtgac
     9781 cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg aattttgcga
     9841 ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat ctctattgat
     9901 ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag ttttaaatca
     9961 acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt agcttcaaga
    10021 aataagaagg aatttacgaa aattgcttct caaatttttg ggaaatttcg gcttattttc
    10081 taagaaatta cttctggaac accgtttgtt cgaaaaaaga gctgcgacaa gaattttgtc
    10141 ccagctcttt tttttttatt ttcctaaata aaattcccgc atcgtttttg caatcatttt
    10201 gatcaaagga tgatcttctg tgaagctaga agataaataa attgttcgtg tcaagggcaa
    10261 atctttgatt tcccgcaaga cgaaaggatc atttttatcg taagaccaag aaacttttgg
    10321 gaaaaagcta acagctaatc cttgcttgac aatagaacgt aatgtcgcag gatcatctgt
    10381 cgtcgaactg atcgtcaatt cgatactttt cgcttcaagt gctgcatcca gtgtatgacg
    10441 gaaggcgttg ctctttgaca gcatcaagat gggggtgcgt tggatatctt ctaattgcag
    10501 atgttctttt ttagcaagag gatgtttctt aggaatggcg acataaacag gttctttcat
    10561 gataggaata ttggtcatgc caggcttcat ctccgaagtg atgtacagta caggatcggt
    10621 aatatcatct ggtaaatgat gttgcatgat cgttaattgg atatcaggat gcttttccat
    10681 aaaaattcgg accaagttcg gaatagaaac agaggctacc tctaaataca atatgacgga
    10741 agtaacgatc ttctcattga ccgaagacaa taatttgatt ccttcttcta atgtgtctaa
    10801 tgtcttgact gcatatggat aaaattcttc tgcataaacg gtcgtctcaa tgtttctgcc
    10861 gctatggtaa aacaaaggga cacctaattc gttttccaaa tgtttgatcg cacggctcaa
    10921 ggcaggttga gaaataaata attctttggc tgcttgggta acattttttg tttcgaatac
    10981 ttttttaaaa tactgcagat gaagcaattt cataattatc catcccatcc ataccttttt
    11041 gttatggttt tttctttttt tggcattttt tattatagca gaaattgact aagatagcag
    11101 tggaaaaagt aagtgctgag gaggcgaggc tcatgagtac aggtttctta tttcctggac
    11161 aaggcagtca gaagttaggg atgttagcgg atatccctga aatgtacatg gaacgtttag
    11221 aaaatgtgac ggggtatcgg ttagttgata aagaagaaaa ctatcaagat acggttttta
    11281 tccagctagc attactgaca aaagcagtat tttatttaga tgaaatggaa aaaaacggta
    11341 ttcagcctga tattgcagca ggacattcga ttggtgcttt ttgtgcagct gttgcttgtc
    11401 gatctttgag atttgagaat gcagcggaac tagtctatca ccgcgcagtc ttaatgaaag
    11461 aagcttatcc aagcggttat gcaatgggcg tagtagtagg tgtgacgaga agcgaagctg
    11521 aggaaattgt agcaaaaaca tttgatcctg accatccagt ttatctgtca aatgaaaatt
    11581 gtccgatgca acatacgtta tctgggagta tcgaaggttt atccgttacg ttggcagaag
    11641 ccaaacggca ccaagcgcaa acagcgaagc tgctgaaagt ccctgtgcca tctcactgtc
    11701 tcttgatgaa tgatacggtc aagaaattca agccgtatat gcagtctgtc catctatctg
    11761 atgctagatg tttgtatata aaaaatacgg atggaagagc cactcaggac gcagaagaga
    11821 ttcgtaaaga tttgttggaa aacctagctt tccctgtgca gtggaaccaa atgatggact
    11881 tggcaaaaga attaggaatg gatctgacga ttgaatttcc ccccggcaat actttgacaa
    11941 agctgattca tacaaaattt ggtgtagata aagggatccg tacgatcaat ctagaccaac
    12001 atggtattga tgacgccacg tttctatatc aaaaatggag atgactaatt atggaaaaac
    12061 caaaccgcaa ctgggcttct aaacgcgaaa aaaaagcgca gcgattggaa aaaatcagca
    12121 acttgctcaa tggcaagtat gtagaaactg aaaaaatcgt agaagtaatg gaaaaactga
    12181 ttgctcccgg cgacaaagtt gtattagaag gcgacaatca aaaacaagca agttttcttt
    12241 cagaaagtct tgaaaaagta gatgcagaaa aagtgaatga tctgcatttg atcatgtcta
    12301 gtatctcacg accagaacat ttgaatattt ttgaaaaagg aatcgcaagc aagatcgatt
    12361 tctcttatgc tggtgcacaa agcttgcgtg tcgcccaaat gattgaagac ggcacaatga
    12421 aattaggtga tatccatact tatttggaac tttatggacg cttattcatc gatttgattc
    12481 caaatgttgt tttagtagct gcagataaag cagataaaga cggcaacttg tacacaggtt
    12541 tcaatacgga agaaacacca acaatcatcg aagctgcagc tttcaaagat ggtatcgtga
    12601 tcgttcaagt taacgaagtt gtcgattctt taccacgtgt agatatccct ggtgactggg
    12661 tagacgttgt cgtgaaagca gataaacctt atcaattaga agctttattc acacgagatc
    12721 ctcaaaacat cacagaattg caaattctaa tggcaatgat ggcaatcaaa ggaatctacg
    12781 cagaacacgg cgtacaatct ttgaatcacg gtattggatt caatacagca gctatcgaat
    12841 tgttactgcc aacttatggt gaatcactag ggttaaaagg gaaaatcgcg aagaactggg
    12901 tattgaaccc acatccaacg atgattccag ccatcgaatc aggttgggtt gaaagcatcc
    12961 atagttttgg tggcgaagtt ggtatggaaa aatatattgc tgctcgccca gatgtctttt
    13021 ttgtcggtaa agacggcacg atgcgcagta accgaacatt atcacaagct gcaggacaat
    13081 atgccatcga tatgtttatc ggttcaacct tgcaattaga ttacgaagga aactcttcta
    13141 ccgtaacaaa aggacgttta tctggttttg gcggtgcgcc taacatggga cataatcctg
    13201 gcggtcgtcg tcattctaca ccagcatggc aatcacttcg cgacaatgat gatccattag
    13261 ggaaaggcca aaaattagtt gttcaaatgg ttgaaacata tggttcaaat aaaaaaccag
    13321 tctttgtcga agaattagat gcactagctg ttcaaaaaca agcaggctta gccaatgcaa
    13381 tgcaccagtg atgatttata gcgaagatac tacacatatc gtgacagaag aaggaattgc
    13441 ttatttgtac aaagcaaaaa caaaagaaga acgacaagca gcaattgcag caattggcgg
    13501 tgttacacct cttggaatga ctagcacgaa agaatcacta gatagcttgc gtgcagccgg
    13561 catcgtgaaa ttaccagaag atctaggcat caaacgtagt gaagcgaaac gttcattatt
    13621 agctgcacaa accatcgatg atttgatcga atggtcagat ggcctgtatg aaccaccaat
    13681 gaaattcaga acttggtcat aaaattggcg agaaaaacct tttgtcgcca tttacccaga
    13741 tggaagaaga aagacgaata agcagaacgt gtatgctctg ttttacaaaa aggggagaga
    13801 tccatggaac aattagctta taaatatcca acgaaacagc cgatcaataa gaaaatccat
    13861 gtaggagtgg tcggttcagg agatttggaa atcttgatgt ttccaacaga aaaaacagaa
    13921 tctactgtaa aaatttgtac aggaagcgat ggcttcggcg aagtatggaa caatgtatta
    13981 acgagatttt ttgatcgtta tccaatttca gcagatatcg tgatcaatga cttcggtgct
    14041 acaccaggag ttgtgtacct acgattgacg caagcaatgg aggaactttc agatgaaaaa
    14101 tagttttgta gaattaaacg ccagagaacg agtgaacgct ctacttgaca atggacgtga
    14161 attgattgat ccattcgatc aaatcatcgc gccaagttta ccagcacaag gaatcgtccc
    14221 tgaaagtgat gacgggatcg ttgtcagccg tggcacgatc aatggcaaac aagcagtagt
    14281 aatcgctatg gaaggaaaat tccaaggtgg cggtatcggt gaagtttccg gtgcaaaaat
    14341 cgttgctgca cttgaacatg tgttagaaga aaataaaaaa ggaaatgaga tctttccagt
    14401 cattgtacta gatacaggtg gtgttcgttt gcaagaagca aactacggac tgttatctat
    14461 ttcagaaatc ggaaatgtaa tcattgctct aaaacaatat gtaccagtca tcggtttgat
    14521 tccaggacgt gtaggatcat ttggcggtat gtcgattact tcagcattaa tgagttattt
    14581 aatcgcaaca aaaaaagcga gagtcggttt gaatggacca gaagttattg aacaagaagc
    14641 aggcgttcga gaatttgatt ctagtgacaa agacttgatt tggaatacat taggatctcg
    14701 tcaacgggta gaatcaaaaa tcattgatga attagtgact gatagtgtag aagcaatcaa
    14761 agctgctgtc atcgcagcaa tggacgaacg aaaagacacg catcgcagtg aaagctcagc
    14821 attttattta tcattacttg attctttaga tctttcaaaa ccaatggcca ttgacgatta
    14881 caatgaagca attgctaacc atcaagcaaa atcaattgac ttaccagaag tagcagaagg
    14941 aaatgaacca gctgcagcaa gtgttggtta cgattggttt gcggcactaa ctggcatgaa
    15001 aaatccaact tcttctatta gtactgtgta ttatggtaaa tcaagtaaat tcatagaaga
    15061 agccatcgtg acatcaatcg tacctgatcc taaaaacccg atgtaccgtg tacgaaacgg
    15121 tgaagttggt ttagttgaag gtttccgcat gggacaaatt ttggatcacg tttacgaaga
    15181 agataaagac aaaaaagtga aacggccaat catcgttgtg atcgatgtac caagccaagc
    15241 atacggctat aacgaagaat tgatcggtat ccatgtggct ttggctaata gtgctgctgc
    15301 ttatgcaaaa ctccgtcaag ctggacatcc agttattgga ttgattgcag gaaatgctat
    15361 ttcaggtgca tttttagcac atggattaca atcaagccgc ttgattgctt taaacaataa
    15421 agaaattacc gtccaagcga tgtctaaaga aagtgctgca cgtgtgacaa aacgttcggt
    15481 tgctgaaatc gaagcagctg ctgaaaaagt tccagctatc gcttacgata tcaataatta
    15541 tacaaaacta ggagcgatct atcatttcct agaagatatc accgaccaag ctgcttcaga
    15601 acaaaatgtc agcaaagtca tcgaagcaat caatgaagcc attcaagatg tccgcaatac
    15661 aaatgacacg atgctgacaa atcgatacac aaacgaaaca gccaaaacat tcggacgtgt
    15721 cgaaacaaat aaagtccgtg ccaaaatgga cgaagaatgg gatgcataaa aaatgattca
    15781 agcacatgat attgtccttt ttgataaaca tgaaatagaa gggttcacct tgcctgaatg
    15841 ggtcgttcta actgaaaaga tgttcggaac agttcgtcgt gcgtcgacgg atcacgcaga
    15901 aatactaccg attggtttac gtggacctgc ccgcaatcag agatggtcaa cagaaatctc
    15961 aaaacatgcc gtcaaacaag tcattcatcc gtgggacatc atcgaacaag aatcgtatag
    16021 acaagagaaa atcatggatt atcctgttta tcagcaattt catgctgcta gaaacatatt
    16081 atcaggatgt aaatggggaa tcggcggcag cttaggattt gaattatcga ctgggattcc
    16141 ggcagtaaaa gaaaccagtg attttgattt gctgctgtac gcaaattcac cgattgaatt
    16201 gccaattcag gtaattcaat cgcatcctgc tttttttgag caattcgata cccaagtagt
    16261 gactcggcaa ggcggttttt cactaaaaga gtatttgaga gagccagaga aaaaaattct
    16321 attgaaaaca gtcgaaggac ctaaattaac caaagaactt tggtaataaa gaaaaagaga
    16381 gagaaagaga tgtttgaaaa caaaaaaatc aaaatcaacg taagtgactt ccagaaagta
    16441 tcagctgtaa cggctgttat gatgcaaacg atattgagct ttgctttggc aaacagccac
    16501 agcgcaacaa atacagcttt tcttggtacc ttttacgtca tcgttaaata cacagcacca
    16561 atgtttattt ttgcaattgt ttataatatg gtaaaaacaa gtcagcattt gtcttattgg
    16621 gaatttttga aagaaaagtt ttttgaacta gtcgttccgt atcttctatg gacgacggct
    16681 tatctttggc tatttccagc agtccaacaa caaactccgt atacggatac tgcaagcttt
    16741 ttactgaaat atgtcacagg tgatggagcg cctcatttat ggtataccgt tatgatgctg
    16801 cagatccaat tgttcatgcc gttttttgtt tggctcggtt acaaagtatt tgctaataaa
    16861 aaatatgtat ggccagtatt gattggttca acgattttat atgtcgcatg gtatctgttc
    16921 tatcaaacac aagtattgac tgggccttat catgacagct ggtacttatt agatcggttt
    16981 gttgctagtt tcatgattta tggggtagcc gcatttgttt atcacgaaaa agtctatcaa
    17041 tatttggaca gagtacgtta tcttttctta cctgttgcct tagttatcgc gttcttttct
    17101 gtccgttcat tgctcgcaca tccaggagac ttgagctttg cgaatgcacc ttacttgaat
    17161 acgatccaaa gtttatacag tttagtcatc atctttgctg tcttcatagg cgcttccaaa
    17221 atgattgtaa acgattcgcc aaaattgcca ttgttcaaat ggttgtctgt ttatgcttat
    17281 cgtacgtatt tagccaatgt atttgttttc caagtactgt tattattgtt taaagatagt
    17341 tggctgcagt taccttcagg agtcatgatt cttgtagctt atctaatgac ggcaagttgt
    17401 gcatttgctt taagctggtt acttcatatc atttgggtag ctatcaaaaa aggtttcagc
    17461 aagtaatagt aggaagaagg agtgagtgtc atgataaatg acgaacgaaa aaaagcagtg
    17521 actcagcaac tcgctgcgtt ggtagaggaa gcattgatcg cagaagtaac attaagtcca
    17581 aaacctggat tagtggatgc actggatgcg ggtgcacatt ccgatatgga ctatgcttta
    17641 tttcttaagt ctgctaagac gctaactcct ttttttgaag aaatggctca aatagcatgg
    17701 catcatgcta ttgatcagga gttaagagaa agaattgctg agattgggcg aaaagcggaa
    17761 aaggcaatgc tgattgctac aaatggcatc aatacccata aaggagcgat ttgggcaatg
    17821 gggctattga tcagtgtcat tgcacaaaaa atcagtatgg aacagccttt ttctttatcg
    17881 aaaatacttg atgatgtcag caagcttgct gcttttcctg atacaaaata tcgtcataaa
    17941 aaaaccactc atggtcatag ggtgaagcaa aaatatggcg taaatggtgc atatgaagaa
    18001 gcgatattag ggtatccgca tatcaggctt gcaattcaag cagctaagga gctagataat
    18061 gattcatatg tacagaaaca aatgcatatg ttgttagttt tgatgagttc gctagatgac
    18121 acatgcgtat tgcatcgcag tgatcagaaa acgttagtgg aaatgcaaaa acttgctcat
    18181 gctgctagtc aaagtgcttt accaaatcgt ccatttacag cactgttgca cttttgtcag
    18241 gaaaaccaaa tctctcctgg aggaagcgca gatctcttgt ctgcaagctt gttcatgctg
    18301 aagatagagg aattatggac tgccgacaca tacatttgtc cgatgttact caatgactaa
    18361 ataagacaaa attgctaaaa aaacagttta tgcatctggt tattgactct tccttttagc
    18421 aaggctatca taaaaatagt tttttgtaat aaaagataac gaggaagaca atgaaaaaaa
    18481 tcaattattt agcaatcatt ttgttagtgg gcctatgttt gtttgctctg ccgacccaag
    18541 gattcgctgt cgcgggtggt catggtggag gtacatctag cggtcgaagt tcgggcagag
    18601 gttcatttgg tggtcgttca tatgggggca gttattttgg cagtcgttct tacggcagag
    18661 attcttatgg gtattatggt ccaggtacag gtccttctag ttatgagcaa acgggatttc
    18721 ttgcattagc tgttttgtca gtaggagggg tttctgcttt ttctatcgtc aagaataaaa
    18781 gacaaaagaa aaggctgaaa gcggagatat tgtcccgcat gtcaggaacg gctaaagaaa
    18841 agaaagcaca gtttctggaa atccaaaaga tattcatgaa gatccaatcc gcttgggaag
    18901 aagaaactcc tgaaaaagcc agaagctgtt atacagacaa gctgtttgcc gaacatcaga
    18961 aagttctgca aaaaaacaaa gcagacggtg taagaaatca tactaaaaaa ataactattc
    19021 aggaagtagg aaattatcga cagatccacg aaaatagttt tgccatccga attgatttca
    19081 gatgctgtga ttattcagtc gatcgaaaag atgggcgatt gatctcagga agtaagacac
    19141 aacgacagta tttttctcag atatggtatt ttgatttttc tgagaaagaa aaaagatggc
    19201 aagccgattt tattcagccg attatgctgg attgacagga aatatttatg aacggagttg
    19261 tgacaaaagc cttgtcgcag ctcctttttc tgaataaacg gtgtgccaga gatagcctat
    19321 cggcaataag gcacgatttt gtaaaatatg ggaagcgatt tttcaaaatc gcttcttatc
    19381 gctcaaggct gaacatctct gtcacaacct ctccactaaa tggtgtttaa aagcttgcat
    19441 ctgcgataat aagaacaacc aaacgaaaaa taggaagcac tattttagtt tagttgttct
    19501 tattacttga tgcaggacag ctttttcaca acctcttttt tgtatagatg ggcaaaattt
    19561 ttgtaataat aggcgtatgt ctttttcgtt gaggagatga aaaagcatat ggcttatttt
    19621 ttgtttgctt tgcttacttt ctttccactt caagtggcag aaatatctac tttttattat
    19681 gtaagcggaa ccatatactt gaaatcagaa ggagatcatt tctatgaaaa caagaacaat
    19741 tgaaatactt cacagaatca tgacagccca atatccagta aaagcaaaac atctagcaag
    19801 tgagttcaat gtcagtcagc ggacgatccg tcaggaagtt cttgaagcga atatctggtt
    19861 gagcagtaaa aagcttccgg agatccgtac aattcgcaac aaaggttttt tgttgaagct
    19921 ggaaaaggaa aagcaagctc gattagaaac tgaaataaaa gatgtgaaag acggcttttt
    19981 gaatcggcgc gaacgtattt ttgatctagt tttagccgtt gcttacagcc gaatcccgat
    20041 ccatctaaac aaaaaagaag aagaatttca aatctctaaa agtacattag atgaagatat
    20101 gagacggtta cgagcaacac tggtcagtta tgggatagag atcgtcagtt atggaaagca
    20161 aggactggtt tataaaggtt cagaacgatc tatccgtacg atgatctatg attttatcaa
    20221 tcagaatctg ggaatgaccg acttctttat gaatcaagat gcacaattaa caccggctca
    20281 aaaaatcttt ctccattatt ttcctttggt ggaaatcaaa aaaatacagg agatctattc
    20341 caataagttg ttgaaacaag aagatgatat ttataaaaaa caaatcctgc tttttacatt
    20401 gatctggatc cagcgaatca ggaaacatga atttatctct gccattaatt ggaaaaatac
    20461 agaaataaaa gacagtagct tttctgcatt tattgaccac atgatcgagg aatttcaatt
    20521 ggacaatgtc ccgcaagtag aaagaaatta tcttcgtttc actattgaga cgttcaatgc
    20581 tagagatatc tccaattcaa tcgagtgggt acaagctcaa ttactaacga tccaattgat
    20641 ccaatttgta gagaacgaga cacacgtacc tttccatcta aaggaagaag ctttattcga
    20701 aaatctctat aagcacatgg ctgcactgat tgtaagaatc aagaatcata tccaagtcat
    20761 gaatccattg aaagaaaaca tccgaacaaa ttatttccca atctaccgcg cagtcactca
    20821 ttttgttcct acgatcgaag aagtaattgg tggcaagata ctggaggacg agatagcttt
    20881 tcttgtcatc cacttttcga cgatcgccag tgtaatcaag cgagacttga attatgttta
    20941 taaatcagtc gttgtctgta accatgggat ggctactgga aatctgttag cagagaatct
    21001 gaaagaaaag tttcctcaga tcgagattac tgcagtttta agttcaaaag aaatcgaatt
    21061 agtagataaa ttggatgtca atctggtttt cagcaccttt caattgaatt atcgaaataa
    21121 gccgctttta gttgtcgatc cgattttaac tgagacgaat atcttggtta tagaagactt
    21181 tttgaaaaga aatgaagaac tacagcggtt agagcctaac agtcatgaat cgacggagtt
    21241 attcactcaa ataatagatg tcatgaaaga aagtggtggc cgagtagatc gaatcatcta
    21301 tggcaagtta gaaaaattat tcgaaacaaa caatttagaa atcaacaaaa gggagatcca
    21361 gcctatgtta aaagatattt tgacggacag caatatcatg atcaaagaaa aagccactac
    21421 ttgggaggaa tcgattgaac tagctgctag accattggtt aaagaaaaca ttgtcgaaga
    21481 gcgctatgtc gaagcgatga ttcgtgcagt gaatgattac ggaccgtata tcgtgattgg
    21541 caaacacatg gcactagccc atgcccgacc agaagatggg gtcaataaat taggagtcag
    21601 cgtagctacg attgagcaac caatcgattt cggtaatcct gaaatggatc cagtaaagat
    21661 tattttttgt ttggcagcag tagactccta ctcgcatctg aacatcatga aagaactaat
    21721 tgaattgatc aacaacgaag agaagttgga tcgattgatt gaatgcacaa atatcgaaga
    21781 tttcaaacaa ctgctttttt cagtagttga gaaataataa atgatacggg ggagaacaaa
    21841 gatgaacaaa ttgaacattt tattcgtatg cggtgcaggt ttaggaagta gttttgcagc
    21901 acagatgtct gctgaggatg ttttgaacaa attaggagta gaagccaaac tggaccattg
    21961 tgatatttct tccgcagttt ccatgaaacc agatgtgatc atcacagcag aaaattttcg
    22021 ttcgcagttc gaaaagttcg cagttgatcc taaaacggcg atgatctatt tgaaaaatat
    22081 tgtttcaaaa aatgaaattg aagaaaaaat caccccagtt ctgcaagata agggagttat
    22141 ctaaatacaa aaagaaaaga gggtaaaaa
//
LOCUS       DAYYNQ010000048        21707 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000048 DAYYNQ010000000
VERSION     DAYYNQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.048"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(424..1920)
                     /gene="lysS"
                     /locus_tag="ACHBJ1_001644"
     CDS             complement(424..1920)
                     /gene="lysS"
                     /locus_tag="ACHBJ1_001644"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM9430580.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2031..3035)
                     /gene="dusB"
                     /locus_tag="ACHBJ1_001645"
     CDS             complement(2031..3035)
                     /gene="dusB"
                     /locus_tag="ACHBJ1_001645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM9430581.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3036..3923)
                     /gene="hslO"
                     /locus_tag="ACHBJ1_001646"
     CDS             complement(3036..3923)
                     /gene="hslO"
                     /locus_tag="ACHBJ1_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM9430582.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4140..6251)
                     /gene="ftsH"
                     /locus_tag="ACHBJ1_001647"
     CDS             complement(4140..6251)
                     /gene="ftsH"
                     /locus_tag="ACHBJ1_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM9430583.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6341..6886)
                     /gene="hpt"
                     /locus_tag="ACHBJ1_001648"
     CDS             complement(6341..6886)
                     /gene="hpt"
                     /locus_tag="ACHBJ1_001648"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM9430584.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6886..8331)
                     /gene="tilS"
                     /locus_tag="ACHBJ1_001649"
     CDS             complement(6886..8331)
                     /gene="tilS"
                     /locus_tag="ACHBJ1_001649"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM9430585.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8451..8921)
                     /locus_tag="ACHBJ1_001650"
     CDS             complement(8451..8921)
                     /locus_tag="ACHBJ1_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM9430586.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8967..9392)
                     /locus_tag="ACHBJ1_001651"
     CDS             complement(8967..9392)
                     /locus_tag="ACHBJ1_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM9430587.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9459..9728)
                     /locus_tag="ACHBJ1_001652"
     CDS             complement(9459..9728)
                     /locus_tag="ACHBJ1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM9430588.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9739..11334)
                     /locus_tag="ACHBJ1_001653"
     CDS             complement(9739..11334)
                     /locus_tag="ACHBJ1_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9430589.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11349..14870)
                     /gene="mfd"
                     /locus_tag="ACHBJ1_001654"
     CDS             complement(11349..14870)
                     /gene="mfd"
                     /locus_tag="ACHBJ1_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM9430590.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14887..15447)
                     /gene="pth"
                     /locus_tag="ACHBJ1_001655"
     CDS             complement(14887..15447)
                     /gene="pth"
                     /locus_tag="ACHBJ1_001655"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM9430591.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15800..16774
                     /locus_tag="ACHBJ1_001656"
     CDS             15800..16774
                     /locus_tag="ACHBJ1_001656"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM9430592.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16944..18344
                     /locus_tag="ACHBJ1_001657"
     CDS             16944..18344
                     /locus_tag="ACHBJ1_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM9430593.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18422..19144)
                     /locus_tag="ACHBJ1_001658"
     CDS             complement(18422..19144)
                     /locus_tag="ACHBJ1_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430594.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19223..19729)
                     /locus_tag="ACHBJ1_001659"
     CDS             complement(19223..19729)
                     /locus_tag="ACHBJ1_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9430595.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19852..21651
                     /locus_tag="ACHBJ1_001660"
     CDS             19852..21651
                     /locus_tag="ACHBJ1_001660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM9430596.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6029 a         4734 c         3893 g         7051 t
ORIGIN      
        1 cgaaatcttt cggcaactca tttatcataa cttatcgttt gttatttgtc aagaactttt
       61 ttgaagtttt tttcgataga atcaagcttc gcaagtggtt cttttttaac gactcagtta
      121 tcttatcatg tcgttagtaa catgtcaata acttttttgt tatgtttttg aataaatatt
      181 ttcttctgtc ttttggacaa gtaataatat accaactaaa tttagcttgg tcaacgaatt
      241 ttttgtattt tttcaagttt ttttcaataa acgattattc ataacttttc tttctttatc
      301 gttcgttttc gtcttcttta atgtctagaa tagtcggcat tagcttttga actttcgtta
      361 ttatttattc ctttctacac agcaaaagct gcctaaggaa ttccttagac agcttttatt
      421 cgattatctc attgttggga atagcaaaac atcccgaatt gattgtgcat cagttagcaa
      481 cattactaat ctgtcgatcc cgatgcctag accaccagtt ggcggcatac cgtattctaa
      541 ggcttccaag aagtcttcgt ctacaccgtg agcttcgtca tttcctagtt cacgttcttt
      601 ttcttgcgct tcgaatcttt ctctttgatc aattggatcg ttcagttctg taaaggcatt
      661 agcaaattcg cgccccacta tgaatagttc gaatcgatct gtgaatcgag gatcttctgg
      721 atttttcttc gctaaaggcg aaacttcgac tggatgtccg tatacaaacg tcggttgtgt
      781 caatgtttct tcaacaaatg tttcaaagaa ttcattgata acatgtccga cacccatagc
      841 ttcggttact tctacattgt gtttttcggc taaagctaat gcttcttcca atgtcatttc
      901 ttgccagaag tctacaccag tttgttcttt gattgcatct accatatgga tgcgtttaaa
      961 gtcactttct aagtcgacag cttgtccatc atatgtgatt tttgctgttc ctagtacttt
     1021 ttcagctgcg ttacggataa taccttctgt cagattcatt acatcattga aatcagtata
     1081 tgctgtgtat gcttctaaca tcgtaaattc tggattatgt gtagtatcaa ttccttcatt
     1141 acggaaaacg cggccgattt cataaacttt ttccattccg ccaacaatta gtcgttttaa
     1201 atgaagttct agcgcaatac gcaaatataa atcaatgtcc agtgcattat gatgcgtgat
     1261 aaatgggcgt gcggcagctc cacctgcctc attgtgcaat actggtgttt ctacttcaat
     1321 atagccattg ccatctaaat agcgacggat ttcgctgata atttggcttc gtttcataaa
     1381 tcggtcaaag ctttcgcgat tactgattaa atccaagtag cgttgtctgt agcgttgttc
     1441 aatattcgtt aagccatggt atttatctgg cagtggacga agtgcttttg acaaaatcgt
     1501 gatttctgaa gctttgatag aaacttcgcc tgtgtctgtt ttcatgattt gtccagttac
     1561 accaaagaaa tctccaaggt ctgcatgttt gaatacttca taggcctcat cacctacttg
     1621 gtcttttctt acataaattt gaatttggcc ttcacgatct tgtagatgcg caaatccagc
     1681 tttccctttt cctcggcgag tcatcatacg accagctaca ctagcggtca atcccatttc
     1741 tgctagttct tcttttgttc ttggatcaaa tagttcatgc aattcttctg agttgtgcgt
     1801 gcgttcaaat cgttttccaa atggatcgat tccatcttca cgcaattgtt ccattttttg
     1861 acgacgtaca agcatttgat catttaaatc ttctacttga tgttgttgtt cctgtgtcac
     1921 aaaagttcct ccattctcat gttaatctct ttcctataat gccaatgaaa cacaaaaaaa
     1981 gcaagccgaa cttggaattt cggcttgaaa attcacgaaa aacttagaaa tcacaacgtt
     2041 tgtttcaata gtactttttc cgctttttct acaaattgat ccagcaggtc attgatttct
     2101 tgctgttttt ctgtctgatt gattgctact ttcactttcg ctgctcgagg aatccctttt
     2161 aaataataag ccgcatgtgt tctgaattct ctacaagcta ctttttctcc tttgagatct
     2221 gctaagcgtt gtaaatgaag tttagctgta gcaatttttt cggctggtgt tggttcagga
     2281 atcagttccc ctgtttcgag ataatgctgt gtgcggtgaa tcatccatgg attgcccagc
     2341 gctgcacgac caatcatcac accgtctgca ccgacatagt caagcatcca ttttgcatct
     2401 tctggcgttt tgacatcccc gttccccata aatggaatcg tcaaatgttt tttcacttct
     2461 ttcaatactt cccagtccgc ttttccttca tacatctgaa cccgtgtacg tccatgcata
     2521 gcgatggcac ttgctccagc tgattgtgct gctaatgcat tttctacagc aaagacgtgc
     2581 tcttcatccc aaccaatacg catcttgatt gttactggga tatttactgc tgaagaaaca
     2641 gcttggacca tttcgtatac tttctcagga tctaaaagcc attttgctcc tgcttctgct
     2701 ttgattactt tatttactgg acagcccata ttgatatcta ttatatcggc agctgtgttt
     2761 tcttcgacaa attgagcagc ttcaactaga ctatctttat ttccgccaaa tatctgtaaa
     2821 cttaatggat gttctgtttc ttcaatgtgg agcatctcta aagttttttt gtttctgaaa
     2881 tgaatacctt ggtcactgat catttcacat acgactaagc ccgcaccaaa ttctttgaca
     2941 gtgactcgaa atgctgaatt tgttattcca gccatcggtg caacaacgac acgattaggg
     3001 atttccacat tgccaatttt ccatgtgtgt tccacttaaa tccctcctaa ggcttctttc
     3061 ttcaatccat ctaattcttc ttcgctatac ttgtatttgt tattacagaa agaacaaacc
     3121 gcttctgcac cgtgatcttc gtctatcatt gcttggattt cttctgcacc taacgcgata
     3181 attgcttctg caaatttttc ttttgaacag tcacatttga attgaacagg cattttttcc
     3241 aaaatgttca attcttctgt accaagcaat cctttcaata tttcttctgg tgtctgtcct
     3301 tcatctaaga gggtagagat tcttggtgtc gtttttaatt gttcttcgat cttgctgatt
     3361 gtttcttcat cggcacctgg catgatttgg atcatgaatc ctcccgccgt cttgatgctg
     3421 tcgtcagtat tgaccaatac actcagtcca acagctgaag ggatttgttc tgagatagca
     3481 agatagtaag taaaatcttc agcgatttct ccagaaacaa tcggcgtttg cccagaaaaa
     3541 ggttctttta atcctaagtc ttttgtgaca gtaaacattc cttgtgttcc aactgctccc
     3601 cgcacatcga ttttaccttc actatttaga ggaagactga gatgcggatt tttgatgtaa
     3661 cctttcacat ctccctttcc atttgcatct actacaattc ctgttgctgg accatcgcct
     3721 tggattcgaa cagttagttt gtcttctcct ttgagcgtcg caccaagtag cagactaccg
     3781 accaatgtcc gtcccaaagc agctgaggag gtgtgccaag tatcatgacg tctttgtgcc
     3841 tccgctacgg tatttgttgc ttgaacggcg tatgctcgga cgaatccgtc gtgagcaagt
     3901 gccttaacca aataatcttt catttgttat gtactccttt ttccgttttt aaactcatag
     3961 tgaatcatac tagcaactac tgcttttttc aactttggaa tgcttctttt aaaaataatt
     4021 cagaatcatc tagaaaacag gacatcaccc tatttacaac ttaaataaag aaatctcccg
     4081 aaaaccggtt tttgttccgg ttttcgggag tcatatcaga tcaattgacc tatatttttt
     4141 tatttgtatt gatcgtctga atgattatct tctgattgtt gagaatctga atcatgttct
     4201 gctttatttt cctcattcag ttcatgtttt gcttcttcaa aatcacgttt ttcttccgct
     4261 tgtttttcag cgtctttttc ttccaaagca cgttttgctt cttcaaaagt tttcgcttct
     4321 ttttcgcttg gataatcatt tgactcagta ccttcaggca tcacaccgtg ttcaaacaat
     4381 gacttgattg ctttggcatc caatgtttcg aattccaaaa gtttttcagc aatcagttta
     4441 tgctgtgcac gatgagcttc gatgatttct cgagctttat catgagcttc catcaagatt
     4501 tttcgtactt cttgatcaat ctcgaaagcg acttgttccg agtaagcttt tgtttgacca
     4561 tagtcgcggc caacaaacac ttgatggttt ccttcatatt gaacaggacc taaacgatca
     4621 ctcattccat attctgttac catgcttcgt gctagagctg tcgcttgctc aaagtcattg
     4681 gaagcaccag ttgattggac attgaagatg atttcttcag ctgtacgtcc accaagtaag
     4741 cctacgattt gttcaaacat atcttctttc gtcatcaaga actgatcttc ttttggtaaa
     4801 gcgatcatat atccgccggc gcgtccgcga gggatgatcg ttactttgtg gacgacccga
     4861 gcgcgactca atactaatcc gacaatcgta tgaccagctt cgtgataagc aaccatttcg
     4921 cgttcttttt tgctgattac gcgatctttt ttagctggac ctgcaatcac gcgatcttcc
     4981 gcttcatcga tatcagaagc atcgattttc tttttatttc ttctagcagc tactaacgct
     5041 gcttcattca agacattttc taaatctgct ccggcaaaac ctggtgtctg ttgagccaca
     5101 actttcaagt caacgtcatc tgccatcggt ttattccgtg catggactcg caagattgct
     5161 tcacgacctt ttacatctgg acgtccaact aaaatctggc ggtcaaaacg gcctggacgt
     5221 agtaacgctg ggtctagtac atcagaacgg ttggttgcag caatgacgat tactccttca
     5281 tttccgtcga agccatccat ttctacaagc aattggttca atgtttgttc gcgttcatca
     5341 tgtccgccgc ccataccggc accacgttga cgacctaccg catcaatttc atcaatgaaa
     5401 atgatagctg gtgcgttttt cttcgctgtt tcaaacaaat cacgaacacg acttgctcca
     5461 acaccgacaa acatttctac gaaatcagaa cccgagatcg agtaaaacgg tactccagct
     5521 tctccggcaa cagcttttgc taataaggtt ttacctgttc ctggaggtcc ttctaaaagt
     5581 acacctgccg ggatacgggc acctaattca acaaatcggc gaggatcttt cagaaattca
     5641 acaacttcaa ctagttcttg tttttcttct tccgcacccg ctacatcaga aaaacgtacg
     5701 cgattagctt ttttgtctgc ttctttggct tttgatttac caaagttcat gacacggccg
     5761 ccaccgccac cgccgccgcc ttgttgtccc atcatcatgt aaaagaaaaa gatgatgatt
     5821 acgattggca agaagctaag caatagggaa acccatatac cgctgcttga ttcttctttt
     5881 actgttgtag caactttgtt ttcttttgcc agttctgtta cttggctcaa tgttgtatca
     5941 tttggcaaaa tgattgttgt gaaatgggta gtagtcgtat ctgttgaacc taagatagct
     6001 aacccaccgc tgttagcgac ggtctgctgt tctttgtatt caccagtgat cttatataca
     6061 ccatttgcag gctgcacttc aaattctttg atcttgcctg cttgtagttg ttctgtgaat
     6121 tttgagtact cgatatctga cgatcgctgg ccgttattac caaaaatgaa ataaacgacc
     6181 attaccatcg ctaagattac gaggacataa tacaaggcat ttttcatcat gccgttgttc
     6241 tttttgttca tagttgccct ccctaagcgt caatttaatt taacagtttt ttatcaatcc
     6301 tgaccattct attgtaccat aatttaattg agatacagca ttaatttgat tgataaacac
     6361 taggtttcaa tacaccgatg tacggaaggt tacgatatgc ttctgcataa tccaagccat
     6421 aaccgaccac aaattcattt ggtacgttga agccaacata atctgcttcg atatttacta
     6481 ctcttccttc aggtttgtca agtagtgtaa caattttgac tgattttgct ttgcggtatt
     6541 tgaaaagatc cactaaataa gcaagtgttc ttccgctgtc aatgatatct tcaacgatca
     6601 acagatcgcg accttctaca ttcgtatcta agtctttgac gatttttact tcccctgacg
     6661 agactgttgc attcccgtag cttgaaacat ccatgaaatc caattccaaa tacgtatcga
     6721 tagaacgaac gatatctgcc atgaatggta ccgctccttt taagacacct actactaggg
     6781 gattggtgtt ttggtaaatt tctgttaact ctttgcctag ttctttgcaa cgaacttgta
     6841 tctcttcttg tgagatcaaa attctctcaa tatccttttc caacatcagg ttctccttcc
     6901 aattgcttct ttttgatatt tgtaaagaag tatgtagtgt attttatcag tttcttcacg
     6961 aatactcaaa tatgaaaagg caaaaggtat caagctatat atatttcttt tggcatctgt
     7021 aatgatccaa gcatgctgcc tttgtgaatt aggtactttc tgatcgataa aatatctaga
     7081 tacttttttt gtgagattct ctgttaattt aatgcggtct cctgcttttc ttttttcgac
     7141 atacagttgc acatcagcag gaatccaaat gtcatgagaa aatagagacc atgaatctgt
     7201 tgctttgatg tcccctgcat tttcaggttt gaccagacca atccattctg tttcattcaa
     7261 atatattcct tgttctggaa tcagcaacaa gcgctggtct tctgtctggc tagttgcttg
     7321 tttttctttt ttagagagta caacaaaatg atactctctt ttcagtatcc aattattgcc
     7381 aatactgagt tgccattggc tttttttctg ttgccactgg tctaaaatcg actccatttg
     7441 ttgttctttc aaaataattc ctgtttttga gaatgctttg tcgaaaaagg agtaaaaaaa
     7501 atagtatcgt tcagcatctg tcatttcttc aatcatttct gcagaaaatc gaaattgttc
     7561 ctcccactgt tcgacttcct tttcgatcaa ctgtcccata tatttttgaa tgacttggtc
     7621 tgcccattgg atctgttgcg aaaattgctg gaaatgtctc aacgcctgtg tattttcttg
     7681 tttcaattga ggaactacca aatgacgaag ccgatttctc tggatatcta acaattggtt
     7741 tgtctgatcc tcaaagtaaa caagctgcga ttccgctgca taagtatata ggtcttcttt
     7801 gctaaaactc agcaacggac gaatcagtct gcccgtagca aatggttgta cttctttgat
     7861 tccagaaaaa gtatttaact gtcccccacg aacgattttc attaacaccg tctcgatttg
     7921 atcatctccg tgatgagcag tcatcaacgt gtcatattgt ccttcttcca tcacgcgagt
     7981 gaattcttga taacgaaagt tacgagcttc tgtttctatg gcggatttgg ctggatcttc
     8041 ccaaatactt acatataaag ggagattctt ctcttcacaa tatgagcgaa gataggcagc
     8101 ttcttctgct gattcttctc ttaattggtg attgatgtgg acgataccta aagaaaaacc
     8161 gatttggcgt tgcacttgta aaagtaaatg gagcaaaacc atcgaatcaa caccacctga
     8221 taccgctgct aaaattctcg atccttgttg ccagaatcct tgttcattcc cgtgccgaat
     8281 aaatgactgc tgtaatagcg gtgctttttt cccgcctttt tcaacgttca tcctttccac
     8341 accctttctt cctaaaaaaa agagagcggg aaaacgtcta aaacgacaat taacctgccc
     8401 tctgttgata ctatgaccca aatagtcgca ctatcattat ttgcggcttt ttagctgcga
     8461 cgtccgccac ggccaccgcg ctttccttct gtgtttcgct tcaatgaaga taagcggtca
     8521 tcgctatctt tcaaaaatga actcatcaat gaatcgaagt cttctttttg agctgaaggt
     8581 tgactgcgag aaaaagattt tttctgtgct ggacgatttc ggttgtcacg gggatctttt
     8641 tgttgatatt ttttaggacg gctttcttct ccagattcag caggttgttc ctgcgctttc
     8701 cggatagata gtccgatttt tccatcatca tttactgaaa taactttaac tgttacttca
     8761 tctcctacac ttaaaacatc atggatgtct tttacatagc catttgacac ttcgctaata
     8821 tgaactagac ccgtttttcc agcacctaga tcaataaacg caccaaaatt tgtgattcct
     8881 gatacttttc cttgcagttt tgctcctacc tcgattgaca taaaaaaaat gttcctccta
     8941 attatttccc ttttttatat tcctgcttat tttgcagttg tagaactagt tgattgatct
     9001 gtttcatttg tcgagctgtc tgtatttgat tctaatatag gataaacttt ttcaccgtct
     9061 tttgagtaaa agaatttgct tctggcaagt ttagctacat actcatcatc tttcagcaat
     9121 gaaacatctt ggtgcagctg atcaacgtct gcgttgactt ctttcatttc agtatttgct
     9181 tcgactctca attcgttcaa ttgatgcatc ctttgcatgt catggaaaat ctgcacacct
     9241 attatcgcga aaatgaccaa agcaccaaca aaaattgcag ccagtctgcg tcttcgaaaa
     9301 atcagttgac gttgttgttg ctgaaattca gcatactttt tctttgcata ttccgtatct
     9361 agcgcagcga ttttattgct ttttttcgtc acttggattc gctccaatct ttttgattgt
     9421 ctttttcatt ataccaatat cgtgaaagct tgtctaatct atttctctag tgtttctgtg
     9481 cgattttcag aaataatttc gtacattttt tgtgcatctt cttttttggt tgattcatgt
     9541 aattccgtaa ttttcacttc caacgttttg ttgccaaact ggactttcaa aatatcgcct
     9601 actttgacat cactggaaga tttcgccaat ttaccgttga tttgaattct tcctttatca
     9661 gctacttctt ttgctactga tcgtcttttg atgattcggg aaatttttaa aaatttatct
     9721 aatcgcatat cttattcctc actttttaat ttttttattt aatcgcagga ttttcttacc
     9781 aaaaggcaac attagccatt ctcgtactgt aaacagacga agacgaatca ctgcataaat
     9841 gaacacagaa gccccgatcc caactcctat cacgctgaca aaaaaagcag tggaacgatg
     9901 agttacagga ccaaatacta tattcaatac accataatac agcagcagta ctactgtcat
     9961 acaaagaagg ctgaacatca acttccgtcc aaaatgccgt tctttccaaa aagcatttag
    10021 ctcttttgtt tcaattcgaa caaaatacca aagcgtcaca cctaatccta aaagggtaga
    10081 caggcttgca cctgctgttc ctaataaacc ggtcagaaaa gaagtagtca atccttttac
    10141 caatagtccc catcctgcgc cttttaaaga agggcgaaac gagttcttgc tttgagcaat
    10201 actttgataa gcttggatga ctgcagtgaa agcaatggaa aaaacaaata atacaagcgc
    10261 tgcatttccc gcataatctt taaacaacgc ataattgata taagggagca gcaaggctaa
    10321 tcccaaagac gcagctaatg ctaaagcggt cgtcaatcta agatagattt tggctgtctg
    10381 taaaaactga cgatagatcc ggttggtcaa atgacgagtc aacgcaggca ggaatgtcgc
    10441 acttaatgca gtggcaatca ctaaacctaa ttgtactaaa ggctgacctc gatcatagac
    10501 cccttttgct atcttcgcac cgtattctga cagtccgtaa acttccaaag cattttttac
    10561 aaagaaagag tcgatcagct ggaaaaagat aagcaatcca ctataaatcg aaaccagtcc
    10621 gccttctacc agaaaacgcc ggatcaaggg ctttgacggc tgtttgaaca atggaaaccg
    10681 tcggaagctt aacgctcctc catgaatctt ttgttcatag tagtgaagga tcccatatgc
    10741 gcataaaccg ccaaaaacag ctccactcat tgctgccgtc cctgtttggt acaccgtcca
    10801 tccaagcttt ctaaaagcaa acgcactaca gacaatcaca aaaacacgaa cgaactgctc
    10861 aactacttga gaaacagcag ttggttccat caagaatctt ccttggaaat tccctcgata
    10921 aaaagataga ccaggcatca gcaaaaaggt aaaggaaact acttggatca gcggtttcaa
    10981 ttgtatgtct cccatcatcc aagcaatcca gctgctaaat gcaaagacaa acaaccacaa
    11041 aaagacacct gtccaaaata ctaagggata aagattttgc aaagcttgct ttcgctcgaa
    11101 aggattcttt ctttctgcaa cgtactttga tatgaactgc ggaagaccgg ataaagctaa
    11161 agtcattgct atcccataaa ttggataaac ttgctgataa acataaaaac cttcgtcgcc
    11221 gactaagttt tggaggggaa ttcggtaaag ggcacttaat acttttgcta tgaaagaagc
    11281 aatcgtcaat acaaatgctc cttgcatgac gcgccgcatt tctttatgat ccaaatcatt
    11341 gctccttcct acgctgacag atatttttgc tcacgcaacg ctttgacaaa cttttggatt
    11401 tcctgaagcc atactgcttc ttccatttgg ttggggatcg tcaagcgtat caccatctgt
    11461 tctttttcaa cgcctagact ggcttttagt tttgtcgctg tcagtgcttc gaataactgt
    11521 tccacactat agcgttttgt tccaacttta cttagagtaa agactatatg cgcctcctgt
    11581 ttccttatcg tttctaggag cgctcgatcg ccatccattt tgatctgccc aattgtcaag
    11641 agatgtgcta cttcttctgg atattctcca aaacggtcga gcagatcatc ttctagttca
    11701 tctagcattt cttgcgaatc aagttcacga attcgtttat agatctcgat tttctgccgt
    11761 tgatctgcca cgtatgtttc aggtagatac gcatcgacac cgaggtcgat ctcaacagtg
    11821 gtcttctcca cttgactatt ctttccttgt ttacgagaaa cagcttcgct tagcatttga
    11881 gaatacatat cgaaaccgac tgcatcaata aatccatgct gctgagcacc taagagatta
    11941 cctgcaccgc gaatggaaag atcccgcatc gcaatcttga atccagatcc cagttctgta
    12001 aaatctttga ttgcctgaag acgtttttca ctgacttcgt taaggatctt ctgctgctca
    12061 tacataaagt aagcatatgc gacacggctg cttcttccta cccgccctcg caattggtat
    12121 aacgtggata atcccatata atcagcgttc tcaacgaaca acgtatttgc gttcgggata
    12181 tctactcctg tttcaatgat ggttgtagtg acaagaacat catattgacc ttcaataaat
    12241 tcaaaaagcg tattttctaa ttgcgcctct gtcatttgcc catgagcata accgatccgt
    12301 gcttccggaa cgagttcttg gatttcttct actttttgtt cgatcgtttc tacccgatta
    12361 tataaataga atacttgtcc acctcgtccc atttctcgat ggattgcttc gcgaatagcc
    12421 ccaggatttt tttccataac atacgtttgg atcggataac gattcgcagg cggtgtttcg
    12481 attactgaaa gatcccgcac gcctaacatc gacatatgca aagttctcgg aatcggtgtt
    12541 gcagtcagag tcaaaacatc cacttgtgca cgtaactgtt tcaatcgttc cttatgtttt
    12601 acaccaaaac gttgttcttc gtcaatgatc agtagaccca aatcgctaaa ttcaatatct
    12661 tttgacaaaa ttcggtgggt tcctacaaca atatcaactt gacctgtacg cagttgttcg
    12721 atggtttctt tttgttgctt tcttgttctg aaacgactta atagcccgat atttacgggg
    12781 aaaccttcaa atctttccag catcgtttca tagtgctgct gtgccaagat cgttgttgga
    12841 actaaaaagg ctacttgttt gctttccttt atcgctttaa aggccgctcg aagcgctact
    12901 tctgttttcc cataaccaac atcacctacg agtagtcgat ccattggttt ttctttttcc
    12961 atatcccgtt tgatctccgc tgcacttctc aactggtcat cagtttcaga gtaggggaag
    13021 gcattttcga attctttttg gtaggcgtca tcaggtccaa aagcatatcc tttttctgac
    13081 tctcttgcgg catataattt gattagatcg tcagcgatat cctcgatttt tgaagagact
    13141 tttcgcttgg ttttcgtcca ttcacttccg cctagcttat tgatgcgagg cgctttggct
    13201 tcagaggcga catatttttg gatcagattc agttgagtaa caggaataaa cagtttgtcg
    13261 tcgttttgat acaaaatagt catatagtct tggtgaacgc catctacttc tagcgtttcc
    13321 atcccaatgt atttcccgat accatgattg gcatggacaa cgtaatctcc tgcttttaac
    13381 tcattatagc ttttcaatcg ctctgcgttt gttactgttt gacgtctcgt ttgttttttc
    13441 gtcgtttttt ggaaaatttc tttttctgtg atagctacta ttttttcttg aggaagttca
    13501 aaaccagcct gcaaagctcc ttcaactaat tgtacctgtc ctctgatcaa tgcatttttt
    13561 tctgtttcta ctactaatat gtcttcatct cgaagcattt gttctgcttt gcgtattctg
    13621 tcctttgtcg gaataaagat cacaacagtc tgtttttgct tgcgccaacg gtccatttct
    13681 gttttcagca atggcatttg tccgaagaac tgttgcatcg tccgatattg aaaattatgg
    13741 attgcttgga aacgaagatt tcccatccct ttttggaaga gtgagaaaaa ggttgtggtg
    13801 aacttttcct tctgcacgat tttttgtata tctgctgaaa atgtctgctc agaaaacacc
    13861 cgcaattcgc tgattttttg agtctgccat tctgccgctt ccctttcgat ttcccgattt
    13921 gtttccatga ttcgctgata atcatcaacg actagtaaac tatttgaagg gaaataatcc
    13981 aagatcgtca cagattcttg gtaaagaaga tctgagtaga attttgcttc ttctgtcggg
    14041 acacctgctt gccagcttga agtcagctga ccgaagtaat cttgcaaaaa ctcgcggtct
    14101 gcttcttctg tggtcgcagc aagccgtttt tctagcattt tttgcatttg ctctattcca
    14161 tgttctagat cctctggtcc gtagacttgt tcactcgttg gggaaaccca gatttcttcc
    14221 gttttttcga ttgagcgttg tgtttctgct tcaaaatacc gcatagaatc tatctctaca
    14281 tcaaatagtt ctacacgaac aggataggtt gtcgttaacg ggtaaatatc gataatgctt
    14341 cctcgaatac tgaattctcc cggttttgcg accatctctt ctcgtacata gcccatcaaa
    14401 gtcaattggc gaggaagatt ttccacgtct atctcatcac caatatgcca attcaattgc
    14461 gcctgtagcc aagttttctt attcggcagt cgtttgtgca aagatgcgac tggcaacaaa
    14521 tagatacctg cttttttttc ttgaagtgcg ttaagcgttg caactctctc gcctcgcgct
    14581 tctggagaag aaaaggccat ttcagcagaa agcacttcat ccactggaaa taaatggatc
    14641 tgatcttgcg gaatcacatg ttgcagatct tctgctaact gattcgcata atacaaattt
    14701 ggcatcagaa taatcatgtt tttatttgtt ttcttaaacg ctcgagcaaa tagaagtgtt
    14761 ttcgctgagc ccgctaatcc tgtgattagt tgtctaggca ttttttcttc taaattgctg
    14821 acccattctt ttacttgcgg atcttttccg actaattgaa taatatccat tccttttcct
    14881 ccttcctcaa ttgaatcgat tcatcgcatc cacgaacgaa tgcccttcta tgagatattc
    14941 gactgcttta acagattgac acatgctttc ttctatcaaa ggctgatttt ctttgctgaa
    15001 aggactaagc acatgctgga ctactgtttt tttcccttct ggcctgccga ttcccacctt
    15061 gattcgatca aagacattcg tattcaaatg ggaaataata cttttgatac cgttatgccc
    15121 gccggcactt cccttttgac ggagccggat ttttcccact gccagatcca aatcatcgta
    15181 gattacgact aattcctctg gataaatccc aaaataagtc attagtgggc cgactgcacg
    15241 accagattca ttcatgaatg tttgtggttt gactaaaagg atcttttctc cattatgaaa
    15301 aaattcggca acttctgctt cgaacggatt ttttttgaaa gtagcttgat gctcgcgtgc
    15361 aattttatcc acgaccataa agccgatatt gtgttttgta tattggtatt tggtacctgg
    15421 gtttcctaac ccgacgatca ttttcatttt tgttttccac ctaattctaa aaagcagcac
    15481 aaaaggctgg actgctaatt agcggtccag tccatcgagc ttttttcttt tttacacttg
    15541 tcaatgcata gtataacaca aaacaaggcg tttcggctaa aagagaatcg ttaataaatg
    15601 ataaagaaat taaaaatttc ttcaaagttt aatgaaataa ttgatatata aaaatagtga
    15661 aataacgcac atgtaagctt ttacatataa tacaaccttg ttatctgtta tattttatag
    15721 ccaaatagcg tgacatgggg cttgagaaat gttagcataa gcatgggaaa aaacaaatca
    15781 atggaaggat tgatactaaa tgactgcagc tgcagaaaaa aaagatcatc aaaaagttat
    15841 tcttgttgga gacggcgccg taggttctag ctatgccttt gctctagtta ctcaaaatat
    15901 cgctcaagaa gtaggtattg tggatatcaa tactgctaaa actgaaggag acgcaattga
    15961 cttgtctcac gctttggcat ttacttcacc taaaaaaatc tattctgcta cttacgcaga
    16021 cgcacatgat gctgacttag ttgttattac agctggcgca ccacaaaaac caggtgaaac
    16081 tcgtttagac ttagtaaaca aaaacctaaa aatcaaccgc gatgttgtca cacaaatcgt
    16141 tgaatcagga tttaacggta tctttttagt tgccgctaac ccagtagata ttttgactta
    16201 ctcaacttgg aaattctcag gtttccctaa agaacgcgta atcggttcag gtacctctct
    16261 tgactcagct cgtttccgtc aagcacttgc ggaattagtt gacgttgatg cacgtaacgt
    16321 ccatgcttac atcttaggtg aacacggaga ttcagaattc ccagtttggt cacatgcaaa
    16381 tgttgccggc ttgcaaatct atgaatgggt aaaaaacaac ccagaaatcg atgaagaagc
    16441 tatggttaac ctattctttg gcgtacgtga cgctgcttac acaattatcg agaaaaaagg
    16501 cgctactttc tatggtatcg cagtagcatt agcacgtatc actcgtgcca tcttagatga
    16561 tgaaaatgct gttcttcctt tatcagtatt catgaatggc gaatatggat tgaacgacat
    16621 ctacattggt gcacctgctg ttatcaaccg tcaaggtatc caaaaagtaa tcgaaattcc
    16681 tttaagtgac agcgagcaag accgtatggc tgcttctgct aagcaattaa aagaaattct
    16741 agatgaagct tttgctaaac tagacgctga ataatctttg aacatgacgg aaaacatcta
    16801 ttcggtgttt tccgtttttt tattttatca cccttatggt ttctaaattt tttcataaag
    16861 tagagataac cttcgtcttt tcctctaaat tatgctatca tgagaccgtt ccaccctaaa
    16921 cttttactta aggagttgtc gatatgacaa gatcttcgca tcgaaaaaaa tcgaaacatg
    16981 ttgctttgct ttcagttctt ggtgttctct tagtgctgat cagcttttat acctatcgca
    17041 gtacatacta cacaaagcat tttttgccga atacagaaat aaatggaata aatgtcagca
    17101 atctaacagt cgaaaaagca aatgaaaaat taaaagaagc ttattctgat aaaaaactgg
    17161 ctattaaaga agatggaaaa gtctggcagg aagtaccaaa atccgagctc ggctacaaag
    17221 atgatttcac ttcggagctt tctccgatcc taaatgaaca aaacggatgg acttggggaa
    17281 tgacttatgt ctcagcggca gaaaaacaag aaatagatcc tgtcagtcaa aaccaccaaa
    17341 aactagacac taccgttcag acattgacaa ctaagctgac tgatttgaac aaagaccgga
    17401 cacctactac tgatgcaacg atcgaaaaag caggtgactc tttcgttatc aagcctgaag
    17461 tcaatggaaa tacgatcgac gtcgatgctg cggtcaaaca attaaaaagt gcggtaaact
    17521 ctggtaaaga tacaattgaa ttgactgaat tcaaagaaaa accaaaagta acatctgaag
    17581 acagctcact gaaagaacaa ctcgcatcca tgaatgctat cgcaaatgta aaagctactt
    17641 acagtataaa cggtgagact ttccagatcc catcaagtga tattatgagt tggctgacct
    17701 acaatgatgg aaaagtcgat ttagatacag aacaagtacg tcaatatgta acagatttag
    17761 gcacaaagta taacacaagt acgaatgaca caaaatttaa aagtacaaaa agaggagaag
    17821 ttacggtccc agttggcact tacagttgga cgatccaaac agacagtgag acagaagcat
    17881 tgaaaaaagc gattctggca ggccaagatt ttactcgttc acccattgtg caaggtggca
    17941 caaccgctga tcatccattg atcgaagata cgtatatcga agttgatctg gagaaccagc
    18001 atatgtggta ttacaaagac ggaaaagtgg cccttgaaac agatatcgtt tccggaaagc
    18061 caactacacc tacacctgct ggagtcttct atgtttggaa taaagaagaa gatgcaactt
    18121 taaaaggaac aaatgacgat ggaacacctt acgagagtcc ggtaaactac tggatgccga
    18181 ttgactggac aggtgttggt atccacgatt cagactggca acctgaatac ggtggagact
    18241 tatggaaaac ccgcggttca cacggctgca tcaacacccc accaagtgta atgaaggaat
    18301 tattcgggat ggtcgaaaaa ggaacgcctg tattggtctt ctaacaaaaa taatatataa
    18361 aaagaaagca cccgtacgat tggtacaggt gctttctttt tattatctaa caatgtgcta
    18421 cttacatttc agcagcagta aagacttctg aaacatcatc atcgtcttct agcttatcta
    18481 ctagacgttc caattgctct ttttgttcct catttaaatc aatcgtcgtt tgtggaatca
    18541 tcgtcaattc tgcttgcgcc aaagtgaact ctttttccaa ctcatctcgt acagcagcaa
    18601 aatcttctgg tgcagtataa atctcgaata cttcaggaga agtttgcaga tcttctgccc
    18661 ccgcctctaa tacatcctca aacatcgtct cttcatcaac agtcaaccca tcgcgttcaa
    18721 tagcgatata tcctttgcgg tcaaacaagt aactgactga acccgtttct cccagtgatc
    18781 caccgtttcg agtaaaagct actcggacat ttgtcgctgt ccgattacgg ttatctgtca
    18841 atgcatggac taagattcct actccacccg gaccatatcc ttcatacgtg atttcatcgt
    18901 aattcgtttc atctacggca ctcgtagcct ttttaatcgc ccgttccaca ttgtcgttag
    18961 gcatatttgc agcttttgct ttgtccacaa caagccgcaa agctggattc atagatggat
    19021 caggaccgcc tgctttagct gccatataaa tttcacgaga aagtttctga aagactttcc
    19081 ctcgttttgc atcttgggca ttttttcttc cttggatatt atgccattta ctatgtcctg
    19141 acatgatttt tcctcccttg ttcttttgag aatacacttc tattctagta ctatttcaca
    19201 aaaaaacaaa gccacagcca atctataaac gcttcttgaa tgccgcaaaa agcacacata
    19261 atatcacagc agtcagagca ccgatcgaat ccaacatgac atcttggaac ataggtgttc
    19321 gtccaccggt catcatctga tggaattcgt ccaaacctgc gtaccctgtt gctgaaagcc
    19381 aagcaaggat cgctgtcagc caccaaattt ttagcttggg gaaaacacct aaaaacaagc
    19441 tgcctcccaa aataaaatag gtaccgaaat gtgcgccctt ccttaaaaag aactctaaaa
    19501 atttggcgta gcccagatgg ttgatactca ccatacttcc accataatta aaacgtatac
    19561 ccgctaatgt gtctttaaat ggctgattag gcagccagct ttctaatcgc gaaacttgtg
    19621 attgctgtcc gtaagtttga gaagaactgt aaaataagat tataacgact atcagtgcga
    19681 ttactaaata aatatttccg tttttcattt gtttttttga agaattcata agtcacctct
    19741 gtattttatc ttagactttt tttgaagaaa caacaatcat tttcatagag acttcttcca
    19801 taaaaaagtg ctatacttaa ttttgtatta caatatgaag ggagtttttt catgagttat
    19861 tcattaaatt ggaatctaga ttctattttt tccggaggca gtcattctga tgctttgaac
    19921 caacggatga agcaattgga agaccaaatg aacgagtatt atcaccgcgt aacaaaatgg
    19981 tcacctagtt ctgacaatgc agagcagtta aacgctattc ttcaattgca agaaacgatt
    20041 acgaatggtt ttacccaatg cagcagctat attactgcat tgctttctgc caatgtcaat
    20101 gattcagatg caaaggtctt atcaggcaag ctttacgcaa tgctgcctcg tctgcaatca
    20161 gccgaaactg tattgtcaaa gaaatttgca gaaatatctg acaatgattg gaatcagatg
    20221 ctttctcaat cttcgtttga aacaattgct tttcgtttaa acgagattcg tcgtgatggc
    20281 tcacaattat tatcagaagc tgaagaaaac attataaata cgctttcttt agatggtcta
    20341 aatgcttgga gcagtcatta tgatacgatt gtagccagca tttcgattcc cttcgaacaa
    20401 gatggacaag tagtcgaatt atctgctgga caagccttca ataagatgat gggcgatcca
    20461 gatccaaaag tcagagaaac acttttcgca gcatgggaag aagcctggaa agaaaaggca
    20521 cccctcttca cggatacttt gaatcattta gatggtttta gactttctga ctatgaactt
    20581 catggtgtca cagattttct acaaaaacca ttagagtaca atcggttgaa gaaagaaaca
    20641 ttgtctgtca tgtgggatac aatccagaaa aacaaacaac cccttgttaa ttacttgact
    20701 agaaaagcta atcttttcgg taaagaaaaa atggaatggc aagatcaaga tgcacctatt
    20761 attttaggcg atttgaaaga aaaaaccttt agttttgacg aagcagcagc atttattatc
    20821 gaaaacttta acaaattcag tccaaaaatg gctaaatttg cccaatctgc ttttgaaaag
    20881 agctggatcg aagcagaaga tcgtcctggg aaacgtcctg gtggttattg tactgagtta
    20941 ccagaaacaa aagaatcaag gatctttatg acctacagta attctgttaa tgaggttgcg
    21001 acacttgctc atgaactagg acatgctttc catagcagca cgatgtggga tttaccttct
    21061 ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa gtacattcgc agaattgatc
    21121 gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag aaaaaatcaa tttgctagat
    21181 acaaaactgc aaaatgcttt agctatgttc atgaatatcc attcccgatt catttttgaa
    21241 aatcgattct atgaagcacg gcaagaagga ttagtctcag aagataagat cacagagatg
    21301 atggttgagg cgcaaaaaga aggctatcaa ggagctttag ctacttacca tccatatttc
    21361 tgggctgcaa aattgcattt ctttattgat gacgtaccgt tttataactt cccatacaca
    21421 tttggctacc tctttagttt gggcatctat gcttacgcca accaaaaggg agcaaatttt
    21481 gaagaagaat atatccattt actacgcgat actgcctcaa tgacgacgga agatcttgcg
    21541 aagaaacatt tgaatgtcga tcttacccaa ccagattttt ggcaagcagg tatcgacatg
    21601 gtgactgaag atatccaaac gttcatggaa ttgacagaag aatttatctg atttctattt
    21661 tatcacaata aaaaaagagt tagtccagaa tgggctaact ctttttg
//
LOCUS       DAYYNQ010000049        20922 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000049 DAYYNQ010000000
VERSION     DAYYNQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.049"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..3396
                     /locus_tag="ACHBJ1_001661"
     CDS             118..3396
                     /locus_tag="ACHBJ1_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM9430597.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3937..5844)
                     /locus_tag="ACHBJ1_001662"
     CDS             complement(3937..5844)
                     /locus_tag="ACHBJ1_001662"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM9430598.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5974..6849
                     /locus_tag="ACHBJ1_001663"
     CDS             5974..6849
                     /locus_tag="ACHBJ1_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM9430599.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6929..7903
                     /locus_tag="ACHBJ1_001664"
     CDS             6929..7903
                     /locus_tag="ACHBJ1_001664"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM9430600.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8083..9228
                     /locus_tag="ACHBJ1_001665"
     CDS             8083..9228
                     /locus_tag="ACHBJ1_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9430601.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9365..9709
                     /locus_tag="ACHBJ1_001666"
     CDS             9365..9709
                     /locus_tag="ACHBJ1_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM9430602.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9980..11266
                     /gene="efmA"
                     /locus_tag="ACHBJ1_001667"
     CDS             9980..11266
                     /gene="efmA"
                     /locus_tag="ACHBJ1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM9430603.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11404..12003)
                     /locus_tag="ACHBJ1_001668"
     CDS             complement(11404..12003)
                     /locus_tag="ACHBJ1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9430604.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12407..13528
                     /gene="mnmA"
                     /locus_tag="ACHBJ1_001669"
     CDS             12407..13528
                     /gene="mnmA"
                     /locus_tag="ACHBJ1_001669"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM9430605.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13774..15195
                     /gene="arcD"
                     /locus_tag="ACHBJ1_001670"
     CDS             13774..15195
                     /gene="arcD"
                     /locus_tag="ACHBJ1_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM9430606.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15209..16327
                     /locus_tag="ACHBJ1_001671"
     CDS             15209..16327
                     /locus_tag="ACHBJ1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM9430607.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16387..17679)
                     /locus_tag="ACHBJ1_001672"
     CDS             complement(16387..17679)
                     /locus_tag="ACHBJ1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9430608.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17829..18389
                     /locus_tag="ACHBJ1_001673"
     CDS             17829..18389
                     /locus_tag="ACHBJ1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9430609.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18518..18895
                     /locus_tag="ACHBJ1_001674"
     CDS             18518..18895
                     /locus_tag="ACHBJ1_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM9430610.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18828..20900
                     /locus_tag="ACHBJ1_001675"
     CDS             18828..20900
                     /locus_tag="ACHBJ1_001675"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9430611.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6777 a         3793 c         4466 g         5886 t
ORIGIN      
        1 tatatttttt ctttttataa aatacactta ttataatttt gttttttatt gacagtaaaa
       61 ataaattagg taatacttta agtaaacgct ttctaatttg ttacgggagg aatacacttg
      121 ttaaaaaaac gtataaaata cagtgcgcta atgatgaatc tccttatgct cagtaccccg
      181 gttttaattt ctgtcgttga agttgctgct aacgaacaac agtctgtgaa cgaagaaaat
      241 gcacacgtga acattgaagg gatgtcaaca atggaggaaa taccaagaac tagtgaagat
      301 atggatacgg aagaaggaaa tgatcctatg atgaaagaat cagaaaatga aaagacaaaa
      361 aagcaatctg aagaagcaaa tgaagagtgg aaaggggttg ttttcggaga aagcacaagt
      421 gctagtaggt atgccattga accattagaa gacggcgtgc ggctctcttc cacgaataac
      481 ggcggaaaat tccagtcaag cggatcagat ggaatgacct attattatca agcaatcccc
      541 aaagacatca actttaggat gcgggcgacg atcgaaatag aatcttggac ctatacgaat
      601 gcccaagaag gatttgcttt aatggtaaga gacgcagtac caaaaaatca tttgtttggg
      661 caggcatttt attcaaattc atttgccata ctaggcagtc ggatcgaata tgtctgggac
      721 tcggatagac aagaagtggt caacacgagc ggcagcaaat atacgatgcg acttggattg
      781 gggacacggg caatcacagg aatatcatct gaagatccgc aagctgcgcc agctcctggg
      841 agtgtctcag tggaaacaac acctttagag acttcagcag ggttcttgga aaaagaaaca
      901 ggttcttata atatattcgg aaacggtcat gggaatctgt ttccggccac gaactcgatt
      961 actaaactgg acgtagagat gaaaaaaacg aatactggtt tagaggcata ttattacgat
     1021 cctgaaacag gagaagaaat ggcttctgac atcatgtatg actgggaaaa attatacgct
     1081 tcagatgaat cggttattta tgcaggattt gctgcagcaa ggaatatgac gatcaaggta
     1141 gaaggaatcg aagttgctta ctcagatcct gcgacagatc ctcctgggca agaaagacct
     1201 actcgcttga ttgatccgat ttacactgtg acctctagta atcattcagg aaaccaagat
     1261 catactatgt cctttagagc aaatgctgac ggattattga caattaaaaa taaagcaaca
     1321 ggagaggtgc ttaagaacgc aaaaaacttg gctatcactg cgaatcggga atttgattat
     1381 cagacaaaac tgatggaagg aacgaacgaa tttactttca gttttacgcc agataaaaac
     1441 tatccgccag aagaatacgt agagatgact agttatgaaa caaaagagat tcttcataca
     1501 gtggactacc gtaaacctaa atattcgaca gtttatgtaa caccagaagg gtcagatgca
     1561 ggaaacggaa gcagacaaaa tccattatct ctgacacaag cattgagata cgcatatgct
     1621 gggcagacaa tcgtcatggc gcctggaact tattccttcg aaagaggatt gacgattcca
     1681 aaaggcgtta acgggacaag agaaaacccg attcagttga ttgcagagaa aaatccagaa
     1741 aacaagcgtc ctgtacttga ttttaaagga actggaaatg ggatgacact gttcagtcac
     1801 tactggggtt tacgagggtt tgacattaca aatagtgcag ctatgcaaaa aggattacag
     1861 atttctggaa atcataattt gatcgaagag attcatgctt atcggaatgg aaatacagga
     1921 atccaaatca gcggtagctc aaatgatcct ttcgaagcat ggcccgcgca taacttagtc
     1981 aagaactgta catcttttga aaatgccgat ccaggatttg aagatgccga tggatttgcg
     2041 gccaaactga caataggaga aggaaatgta tttgacggtt gtatcgcgta tcataatgca
     2101 gatgacggtt gggacctttt tgctaaaaat gaaacaggtt cgatcggaaa agtaatcatc
     2161 aaaaactcag ttgcttatcg aaacggctgg gtaccaggaa ttgagggaga aggtaacggt
     2221 aatggattca aaatgggcgg ttcttcttta acgggccctc atgaattgat caatagtttg
     2281 tctttcgaaa atcttgcgaa aggaatcgat tccaatagcg gaacagatat tatcgtcaat
     2341 agcagtacaa gttttaataa cggttcatat aatgtggctc tttatacctc ttcagcagga
     2401 aacacagact accatgcttc tggtatctta tctttccgaa cagagaatct tgagatgaga
     2461 gagcagatcc gcccattgaa tcaaaacgaa gatcaaatct accactcgtt gaactactat
     2521 tggaatgaac gagagaaaca atcagaaaat actcttggac ataccatatc aaaagattgg
     2581 atcgagtctc taaatacatg ttcgaaagaa ggtcagcagc ctgttacgcg ctatgaaaat
     2641 ggaagtatcc atgttcatgg actgatgcag attaaaccgg ggaatcgtca aacagaaaag
     2701 gaaagagtgg atggtcttcc attatctgta ggtgctattt ttgacggaga aacctcaccg
     2761 tcttcggaat atccagagta tgcaatcgta caagaaccaa atcagacaaa accaaatgaa
     2821 caaaataatc cgattattat acaagaagca aatggtgtgg cggagaatgc agaaggaaat
     2881 atcattgaac tgaagccttt tacagttttt gtgtcaactt tcacaccaaa tcaaggaacg
     2941 cctgtctttg aagatatgcg ttatttccca gaaaagatcc aaatcgttga aacacagtca
     3001 agtgctcaat tttctaatca taccgctatt ttttcagcac cttcaaaagg agaatatacg
     3061 ctagaagtca cttatcgttc ggaaatattt gatggggaaa aatggtctgc tcatacagaa
     3121 tctgttaccc agcaaaaagc gatcactgtg cgagaaactg ccgatgtgcc cccgggaaat
     3181 gacaaaccag aaagagaaaa tccaggaaat gaatcaccaa atggaaaacc aaatggaaaa
     3241 ccttctcatc cggataggac agcaaaagaa agtgaaaaaa atggcactca aggactccca
     3301 aaaacgggag aatttaataa cccgttgcta gcattagctg gaggaatttt gttaatcggt
     3361 gtagttgttt atgtcatgaa acagcgaaaa aaataagtgg aatttctcct tttatgatgt
     3421 aaaacaattg actgaggaaa gtaaaaatag aaggaaatga gaccaataga tgtctcactt
     3481 ccttctatct ttttatagat gaggttctcc ttgctgcttt tgattggctg ttcttattac
     3541 ttgatgcagg acagcttttt cacaatctct ccactaaacg gtgttcaatc agctgaccct
     3601 cggtattagc tcaacaaacg gcaaaatatg aggaactatt tttccgtttt tttctctaat
     3661 actcaggtca ggacgctgat ttcacaacct ctcgataaac ggtgttcaaa agccagtttc
     3721 tcggtagtaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
     3781 ctacttgaaa ctaagcagct ttttcacaac ctctaataca tggtattcca gaagtaactt
     3841 cttcgaaaaa taagccgaaa tgtcacaaaa atttgagaag cgattttccc aaattccttc
     3901 ttatttcttg aagctgacca cttctggcac aacctcttac cagattcgca ctcttttttc
     3961 tggcgctaga tacatagcgt cttgttccgt gataccaaat gtttcataga attcgtcaag
     4021 gttttttggt tgcacgtttg ctcggagttt agcaggggca tggacatcga tttgcagtaa
     4081 aagttgttgg tattcttttt ttgcttttgt acgccaaatc gttgcccaat tgacaaagaa
     4141 ttcctcgata gaaagatctg attccgtttt tgctgcttcc aatgcacagc ttagaccgcc
     4201 tgcatcagca atattttccg aaacggttaa ttttccgttt acttttccgt cagcaaaagg
     4261 cagaccatcg aattcttcga tcattgcttg tgctttgttt tgaaattgtg taagatcttc
     4321 ttctgtccac cagttgttca agttgccata ttcgtcaaat aaagcgccgt tattatcaaa
     4381 tgcatgggaa atctcatgag cgataacagc tccgattcca ccataattag cagagcttga
     4441 ttgttctaga ctgtaaaatg gtgcttgaag gatcgctgcc ggaaagacga tcacattccg
     4501 aaatggatga taataagcat tgaccgtgtc agcactcatt tcccattctg aacgatccac
     4561 tggtttgttc catttgctga aacgatcttt taatgtgagt ttgctgaaaa caagagtatt
     4621 tgaaagcaaa gtaccaccct gatctgcagg tactgttttg aactggtcga ataatgctgg
     4681 gatcttatcg gggtagccta cttggatacc taagttattt aattttatga cggctttttc
     4741 ttttgttgca tccccaagcc agtcattttc ttctagacgt tttttataaa cggcgatcat
     4801 ttttttcacc atttgttcta cgtctttttt agcagcttcg ccaaaatatt tttttccgta
     4861 gtaatcgcca actacttgat cgaattgtcc agaggcaagg taatacgctg cttttttctg
     4921 aggcattgct tcgtctgttc cagacaatac gcgagaatag attccgctga cttggcggaa
     4981 ttcttctgat agataaccac taagagaacg tactgtttta atgatcatcc agcttttgaa
     5041 taacggaaaa tgagcttctg tcaggatgtc attcaaatga tcgaagtaca ctggatcagt
     5101 tacgatcact ttatctggag ttgtcccaat caaacccgtg acaagttttt tcaagtccag
     5161 tttatctgtg tatttaataa atgtatcaaa agattgcgga ttatacattt tgctgtaatc
     5221 tgcgttttct tctgcacttt ttacatgagg agcaatcaat ttatcaaatt gcactgcttg
     5281 ttccgtgatt gcttctgcct gacttttttc tttgccggca agctctaata actgaacagc
     5341 catatcaaag aaaacgccta ttagttgtgc accgttggga tggtctgatg cataatacgt
     5401 cttatctgga aggaataaag aaggcggata agcaaagaag gcgttcgttt gcgcattctt
     5461 catatctgca tctacatcca gattaaatgg caatggtaat gtatccaaca gccattctgg
     5521 aaattgctta ttcaattcgt cgaaagactg caatgattca actttttcga ttataggttg
     5581 aagcggttga gcatgcaact catttcttcg gtcataatcg tttgccagtt gataatatgc
     5641 aaggaaatgt ttcatttttt ctgaagacac tttttctgga tgggcaagca gatcgtctgt
     5701 ttctcccatc aaaagttggt cgatcccgtc aactaaatct tggaaaccgc ctgtagctgg
     5761 tttatctgca gggatttttg ctgttttttg ccattcttca ttgactgctt catagaaatc
     5821 ttgtttcaat cgttctttgt tcattcatcg atcgctcctt ttttgtttcg tgtgcccatt
     5881 atcttacaaa atgaaggaat taacaaatag aacataaaaa attgaaaatt cgaacaatca
     5941 aactgatttg tttttttcaa tccttcactt ataatgaaaa aaaggaggcg atcccaactg
     6001 aatcaagtag tggataaacc tttatatttc aaagtagata aaaaaattaa aaaattagcc
     6061 agcactcagc agctgcagtc aagaaaaagt aaattgcttt ttttagctct ggtttttgaa
     6121 gatcaaagtt atgtgattat cgatcaatca ggacatccgg tagaatattc tccagcaaaa
     6181 tatacttatc aagagggtct ttcttgtaaa aaatggaagc tgatcaatga agagccaatc
     6241 gagttatcta gatggatcaa cagaaaagaa gatattcccg ttttgatcga agaaaaaagg
     6301 tcaggaaagg aactcgcaaa ttgctgggtg ggattgccag aagaacgttt tttgaggtat
     6361 aagaaatggg ctaccccctc aggctatctt tgtggtactt acgcggcagc agttctcctt
     6421 gcctattatc aagactatcg taaggggtgg atgcttccaa atgagattcg caagaaaaat
     6481 acggctgatt cgttggtttt gacgaaggca ttgcgtagcc agatccaacc actaggactt
     6541 cctacgattc cttttcaggt tagtacaggg atttctagtt ttttaaagaa gaatggcaat
     6601 catgagcgtg cacgagcaac tcttctaggt tcatggcagc gagctacaaa acgtatacgt
     6661 gaaggcaaac ctgtgatgat tggtattttg aaagtacttg gcagtacata tggcaatcat
     6721 tgggtgacag cgtacgctta ttttgaaact gaaacaggag aacgttatta caaagtacac
     6781 gacaactggg gcgattatca taaagtcatt cctgccagtt ggagtaacgg aacagtttca
     6841 cttccttaag aatttgctga aattaacaga aaatcttgga aaaatatgtt aaactatcga
     6901 agaatagaga agttttgggg aggactacat gagtaataag tatcaggatg acacgttgaa
     6961 gatttttagt ttgaatggca accgtccatt agctgaaaaa atcgcgaaag tgttcggaac
     7021 agaattaggt aaaagcgtcg tgaagcaatt tagtgatggc gagatttcca tcaatatcga
     7081 agaaagtatt cgaggggatc atgtgtatat cgttcaatca acgaatgcac ctgtcaatga
     7141 ttactatatg gaactactga tcatgatcga tgcaatgaag cgagcaagtg cgaaaacgat
     7201 caatgtcgta ttgccttact atgggtatgc taggcaagac cgcacggcga aacctcatga
     7261 accgatcact gcgaaattga tcgctaactt gatcgaagaa gcaggggcaa ctcgagtact
     7321 gactttagat ctgcacacgg ttcaagtaca aggattcttt gatattcctg tagataattt
     7381 atttacgatg ccgttgtttg ctcattatta ccgtcaatta ggtctgacgg gagatgacat
     7441 cgtagttgtt tcgccaaaaa acagcggtgt tcagcgtgca cgtagtcttt cagaatattt
     7501 gaacagcaca ttagccatcg tagatcatgc agatgatgct tcaactgagg gtggttatgt
     7561 gatcggtgat gttcaaggaa agacatgtat catggtagat gatatcttga acacaggagc
     7621 aacgcttgca cgagcagcaa atgtattaaa agaaaacggc gcaaaagaag tatatgcctg
     7681 tgcgtcacat ggattattgt ccgatcatgc gaaaagtatt ttagacaatg caccaatcaa
     7741 agatatttgt atcactgatt ctgttttcac agaagaaaat cgtcatccag aaaacttaac
     7801 aatcgttaca tgttcagaat taatgggtga agcggtcaaa cgcatccatg aaaatacacc
     7861 gatgagtccg ttattccgtc tggaagaaaa agaattcgaa taaacaaaaa aaagagggaa
     7921 agaagttgtg acagaagtga gcagcttcaa ggagctgcga caagactttt gtcacagctc
     7981 cttttttcta tgcccatgaa acgtcctcta gacaaaaatg agctcttctt tggtttgttc
     8041 gctattttat gctagaatgt gcctagcaat aatttggaag ggatgaatga agtgaaaact
     8101 atttatctgg atcatgcggc aactaccccc atgcatccac aagtaattga agcgatgacg
     8161 gcaatcatgc aagacacgtt tggaaatcct tcaagcatac atggatttgg tcgccacgcg
     8221 catgaaaaat tggaaacagc aagacaaatc attgcagaca gtctgcaagc aaaacctcac
     8281 gagatcatct ttaacagtgg gggaacagaa ggggataata cagctatctt agaaacagcg
     8341 ttctcgcgtc aaaaagaagg cagacatcta atcactacag ctatcgaaca tcctgctgtt
     8401 cttaatacca tgaaatattt agagacaaag gggtttgacg ttacatatct gcctgtagat
     8461 gaaaaaggga atctgtcgat cgaagaggtc aaaaaagcgt tacgagaaga tacgatcttg
     8521 gtctctgtga tgtacgggaa caatgaaatc ggaaatttga tgccaatcaa agaaatcggt
     8581 gaacttttag cggatcatcc agcttatttc catacagatg cggtacaagc gtacggaaac
     8641 caaacgatcc atccagaaga attaggaatc gatttattaa gtatctctgc tcataaaatc
     8701 aatgggccaa aaggcgtcgg tttcttatat aaacgagacg gagtaaatat ccctgcattg
     8761 cttttaggcg gggagcagga agaaaagcgg cgtgcaggta cagaaaattt agcagggatc
     8821 tggggtatgg caaaagcagt tgaactgctg tcacctgaag aacgtgaagc acgtaacaac
     8881 aaatataaag gattccagca attgattctt gatacattag aagcaaacga aatcccttat
     8941 caagtaaatg gtgatttaac gaataaactc cctcatgtgt tgaatcttcg cttaattggc
     9001 gaagcaaatg atttgctact gatgaagctt gatcttaatg gggtcgctat ttctactggt
     9061 tcagcttgta cagcaggaaa tatcgagcct tcacacgtat tagaagcaat gtatggagaa
     9121 gatacaccga tactaaaaga atccatccgt atcagctttg gtttgggaaa taccgaagac
     9181 gacatccagt atttccttga tcaattagtt cgtgctgtca aaaaataact agtgaagtca
     9241 ttcttatatg aacagatgtc tgagagaacg ccttcatttt ttagggaact taactgaaaa
     9301 acctagtaaa aatcaggata aacttttata ataaagagaa actaatttta tcgaggtgaa
     9361 aatcatggca tttgaaacaa cagcaacggt actgggcgca ccaattagct atcgtttgca
     9421 tcctgatgca aaaagatata cattgcgcga taatggattt acagaaacaa acggaggcaa
     9481 ttatcaattg atccgaccat tagatgccac gccgcaaagc aaagaaggat tcaaattaaa
     9541 aatcactgta tcaaaagata ttcaaacatt gaaaatgtcg atcactacag caaatggatt
     9601 gaaagcagtc aatattttca aagatccaaa acagaaaatg agccaagata aattttattt
     9661 tctgatggat gggatgatta gccgtgggtt attcgaaaaa gtcgaatgat ctgtggatat
     9721 aaaaagacaa ctagttttga aacggcctat tgttagatga ctaactttgt aaggacgacg
     9781 caaattagtt gtctttttta tttttaaatt taacacatgt tgttattttt aatattttgc
     9841 tgatttattg tttctaagta aagaaattta aattgaaacg attgttattt agggaaaaag
     9901 gtattttttg aaaaaagaat cccgtataat gattgttgtg caaacgctat acaaaatgaa
     9961 aaagaaaaga gggatgttta tggaaaatga acagtcggtc gttttaacga attggaagag
    10021 aaattatttg ttctttttat cgggacagtt tttgtcagga atcactagta tggtcgtcca
    10081 atatgcgatc atctggtatt tgacaagaga aaccggttcg gcgacaattt tgagttttgc
    10141 tactttacta ggaatgattc ccatggtttt actaagccca tttgtaggac cgctagtgga
    10201 tcgctgggat aaaaaagctc ttttgattgt cacggatatt attgtagcga ttttcgcgct
    10261 tattttagca gtagtcggaa cgatttcaga atccttcccg atttggctag tttttgtgtc
    10321 tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca acgatccagt cgattatgcc
    10381 gacaatcgtt ccatcttctc acataacaag gacgaatgga cagttaggaa tggtccaatc
    10441 agcgaatttc atcattgcgc cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa
    10501 ttaccttatt ttattagatg tattgggggc agtttttggt gtcggcttat tgatttttgt
    10561 gaagatccca aaagtttctc ctgaaatatt ggaagttccg cttactattt ttaaagatgc
    10621 aaaatttggg ctgcagcaat tgatggataa taaagggtta tggtacataa cgatcaatgg
    10681 tgcatttgtg atgctgttgt tcatgccagc aatcagtctg tatccattga tgacgctaga
    10741 ttactttggt ggttccgttg gacaggcggg agcagtggaa gtagtctacg cagtcggaat
    10801 gctcttaggc ggtgcactga tcagttttat aggaacatgg aaagaccgaa tgaaacctat
    10861 tattatagcc tacatcatta tgggcctgac gatcggtgca agcggattgg ttccaaacga
    10921 tagtcaaggg ttcttgtact ttcttatctt gaatgcagga gcaggttgtg caacgccata
    10981 tttcaataca ctgctgatgg caatgatcca gcagagttat gaatcgaatg ttttaggtcg
    11041 tgtcttaggg aacttcaact ctttgatgaa cctggcaggt ccaattggat tattatttgc
    11101 aggaccatta gctgatcgtt taggggtaga aaaaatgttc ttgttttccg gaatcgggat
    11161 tttattatgc ggaatcgttt tatttttaac gtcagctgcc cgaaattatg ataaagaatt
    11221 acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac gaatagtatg gaatggagtt
    11281 gtgacagaag tcttgttaca actccatttc tgatcctaat gttcctgtgg caataagcct
    11341 actcaaatga aaaatatgag taattatctt tatttgttta atacttgata tagggcagtt
    11401 ttttcacgac ctatcgtact tcctctttgc ttcatagatt tctttgattt gttgctcaga
    11461 taacggagtg ccaaatggta gtttttcctt taagagttct ctttccggtg cgtctacgcc
    11521 tacattgaca tcgttctcga taggtacgct gtaatgatgg atatgtccat gaagattgcg
    11581 gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc aagtaatatt gttgatggtc
    11641 gaatttaacg atggcaccta catcataaaa ataaaatttg ggacttccgt cagtaagcct
    11701 aggatcattt ttttctaaat aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa
    11761 gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt tctgcatttg ctttttcata
    11821 ttgcggatgc atagcaaggt cgcctagatg ataaacatgg tcggtttctt ttattactgc
    11881 attccagcta gtgatcaact gttggttcat ttcatccaca tttgcaaaga gccgcggagc
    11941 aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc atatcactga taaagtaatt
    12001 catgtatctt ttgcctccct tttttcttag agtaaacaaa atcacttatc tgagcaactc
    12061 ataacatcta aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga taagaagcaa
    12121 ttttgaaaaa tagtttttca caatttataa aattattcct tattgccgaa aggctggccc
    12181 tttttatgag tatgccaatt ttttatatcg aatgctcaaa agatgagtga ttttttggtt
    12241 gactatcatg tttgtgtact gaataggtcc agtagaacag tatttttacg acttgataat
    12301 taagggatga aattttcaga aatatgacgt ataatgtaaa atactgaaaa gttgcgacct
    12361 tcaactcgta gatgtttcag aatctaattg aaatgatggt gatatcatga cagacaacag
    12421 caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt
    12481 gttaaaggaa caaggatacg acgtgatcgg aatattcatg aaaaattggg atgacacgga
    12541 cgaaaacggt gtatgcacgg ccacggaaga ttataaggat gtagcgaaag tagctgctca
    12601 aatcgggatt ccttattact ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga
    12661 atatttccta gccgaatacc gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa
    12721 agaaatcaaa ttcaaagcat tcttggatta tgcaatggat cttggcgccg attatgtagc
    12781 tactggacat tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg
    12841 agtagacaat aataaagatc aaacttactt ccttagccaa ttatcacaag aacagctttc
    12901 aaaaacgatg ttccctctag gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg
    12961 cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa
    13021 gaacttcaaa caatttttga gcaactatct gccagcaaaa aaaggaaata tggtcactct
    13081 tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat tatacgattg gtcaacgtca
    13141 aggactaggg atcggtggcg gaggagattc gcaagaaccc tggtttgttg taggaaaaga
    13201 tctagcgaca aacacattat atgtggggca agggttccat catcctgcat tgtatgccac
    13261 aagcttggat gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa aagaattcaa
    13321 gtgtacagcg aaattccgtt atcgtcagca agacgttcct gtgacagttc gcttattgga
    13381 tgataatcgt gcagaagtcg tattcgacga acctgtacgt gcaatcactc ctggacaagc
    13441 agttgtattt tatgatggaa tggaatgtct gggtggtggt ttgatcgacc acgcatatca
    13501 agaaacaaaa gttttacaat acgtttaagc gatattaaat gaatcaaaac agctatggct
    13561 aacaaaagaa acaccttttc ttttggatag catagctgtt tttttgcaat aaaattaatt
    13621 tttattcatt ttatacttga aatacttgtg taaaaagttc ataatatcca ataatatgaa
    13681 taaagttgct ttttgttaag caagagcagt tgcaaaatag aagggaatct tttgcaagtg
    13741 atttgttcaa ttttataagt gagggtgatg gaaatggaaa agaaatcgac gggtattagc
    13801 aaatggggac tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc
    13861 acaagtgatc tggctagttc tgcagctcca gggccagcga tcctttcatg gataatcgtt
    13921 gggattggta ttttagcttt ggtattatcg ctgaatcatt taggagaaaa gcgtccagat
    13981 ctagatggtg gtattttcgg ttacgcggaa gcttcatttg gtaaattagg tggcttcatc
    14041 agtggttggg gttactggtt gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg
    14101 atgagcgcca tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt tccttctatt
    14161 ttatttgcaa gtatttttat ttggggtctg actttattag taaacaatgg agttgaagga
    14221 gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag tgccattatt cctattttta
    14281 atatttgtat ttatcgcatt taaagtgaat atattcactg ctgatttttg gggtaacgta
    14341 tctgataatc tagtgaatgg aacagggcag caaggaacga tctggttgca gattaaaggt
    14401 tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt tttagccaac
    14461 cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca ttggtctgct ttgtttattg
    14521 tttatctata tccttgcttc gatcttgcca tacggcgtct tatctcaaga agagttagca
    14581 actatttcac aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca
    14641 gcgctgatca atatcggctt gattatttcg atcattggtt cttggttgtc ttggacgatg
    14701 ctcccagcag aaacaacgat gttgatggct cgagacggtc tgttgccgaa aggttgggga
    14761 aaaacgaata aaaaaaatgc cccaacttat tcgttagttg ttactgcaat cttgactaat
    14821 ctattcttat tgactttctt agttactgat tatgcctatc aatttgccta ttctttatgt
    14881 acagcggcaa ttttgatttg ttatctactt gtcgggatct atcaagtcca gttttcttta
    14941 aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt tgaattgatg
    15001 ggcatcattt tagcaggatt ttcttatgtc cttttatgta gtattgccta tcttcctggt
    15061 ttttgcttct actggatcgc ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa
    15121 gtaatgattg ggctgatttg tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc
    15181 tggatcaaga tataaggggg aaagaaacat gaaaatcgca aatttcggtg tagaagaatg
    15241 gctaaataaa tgggaaaaga aagcggtata tgatattgca caaagctcga tcgaagcatt
    15301 gacattagac gaactcgttg gcttagatgg gactagcgta agtaaatttt tcgaaaaacg
    15361 caaaaacgaa ccattggact atggctggat cgaaggatca gatgttttca aacaagaagt
    15421 cgcttctctt tatcaaaccg tcgatccgga aaacatctta caaacgaatg gagcgactgg
    15481 tgctaatctt ttagctctat acgcgttagt ggaagcagga gaccatgttg tttcgatgct
    15541 tcctacttat cagcaattat atgacatccc caaatcttta ggtgccgctg ttgactttgt
    15601 tcatttgaag gaagaagaag attggcagtt tgatttagaa cagctagaag ccttagtcaa
    15661 acctgaaaca aaaatcatct gtttgaatag tgcaaacaat cctacaggca cattgctaga
    15721 tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat atgtattgat
    15781 agatgaagta tatgcgcctt taacggataa aggtgaattc ttgtcgatcg tagatgtcta
    15841 tgaaaaaggt atagcaacca attccctatc caaaacctac tccattccag gtattcgtat
    15901 tggctggaca gctactggac ctgagttggc agaagttttt cgaaagtatc gagactacac
    15961 gatgatttgc ggcggagtcc tgtcagatga tcttgctgta catgcattga agaataaaga
    16021 aaaaatattg gcgcgaaaca aaaagatcat tacagagaac cttgtgattt taaaacaatg
    16081 ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta cttctttcat
    16141 taaattagcc atcgaagaag atgaccagac attttgtatc aatttattag aagaaacagg
    16201 agtactatta gtaccaggaa gtgcctttga tttaccgaag catgcccgac taggttactg
    16261 ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta tctgagttct tgaaaaaaca
    16321 ggcctaaaca aaaacactgc aaggacagtc tgtgacaact gttcaagcag tgtttttcat
    16381 ttacgcttat tgtctatgag aaggagagtg ccctgaagct atggagactc ctgaagcttt
    16441 ttcttctgca gtttcttgct gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt
    16501 gaccaaaaca agaagagaag tcgaaagaaa gacgccgcga taaccaaatg cactggaaac
    16561 actggagccg atcataggcc ctactacgtt accagttgct tggaaagatt ggttataact
    16621 gaagatccgt ccagcagctt gatgaggcga atattttgtg atcaatgctt ggacagcagg
    16681 gagcagacaa gcatcagaga taccaatcaa aaaacgtaag gcagctaatt gccaaacatt
    16741 cgtgacgaat gccattggga tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat
    16801 tctttcactc ccaatcttgt cacctaagcg accaaatctt ggagcggcta tcaaagtggc
    16861 aatcccaggt atcgaagcaa tgattccgct gatcaacgta acattcccat gtccatgcag
    16921 caattggcga atgtacagac tgatgattgg actaatggaa ttgttggatg cttggatgat
    16981 catcgttgtg acgaacatac cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg
    17041 tttggcagaa accatgtttt cttttttcac tgggacaaat ttttcatgga cgaaaaacag
    17101 actcaacaag aagacgaaaa ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc
    17161 aaacgctgaa gcagagatcc cgccaagaag cggtccaagt aatgttcctg taacagaacc
    17221 agttgctaag gttccaagaa cttgaccact tttttctttt ggcgtcccag ttgcgacaag
    17281 ggcagtggca ttactgatat aaccagaaaa aatcccttga agcagacgca atgcgactaa
    17341 ttgatacaca ctagtcacgc aacccatcaa agaaatgacg attgccattc ctaatgaagc
    17401 acgcaacagc atcaattttc ttcctttttg gtcggctaat tttccccacc aaggagagat
    17461 aagcgtagtc accaagaaag tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga
    17521 cgtactataa ttacctaacg tatctatgta aagtgacata aaaggcatga ctaaactaaa
    17581 tccgatacca gccataaatg ttccaaacca taagacaaac aagttttgtt cccaagtttc
    17641 tcttttccta aataattttt cctttattct tgcagccaat tagactcctt ctttctgaaa
    17701 attttcatct acagctcttt attataaact acaatatgta gtttatcaat gaaagtctct
    17761 tttttctctt tctttttcgt ggtaaactga aagagtgaag gatttatttt atgagaggaa
    17821 acatggaaat gaaaaaaaga aatctgtctc aagagaaaat catcgactgc tttcgagaac
    17881 tagcagaaga gatggatgtt cagcaaataa cctttcagca tttagccaaa cgcttggata
    17941 tcaaggctcc ctcgctgtat aaccatttca aaaatattcg tgatgtcaaa acagcgctaa
    18001 cagcgaaact tctgcaggaa cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg
    18061 gtcaggaagc actgcagatc tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag
    18121 cagtgtatga gttactgatc agtgtaccgc ataccaatga agctgtttta ttagatggaa
    18181 tccatgaaac gaatcagatc attttgcaga tatttgaggc ttttcaatta acgaaagaag
    18241 aaaaagttca tcgaagcagg gaacttcgta gtatgatcca cggttatctt tccttacgtt
    18301 ttttaggata ctttacaaaa gaaccaacaa tcgatccaga agacagctat ttttggatga
    18361 tcaataactt tatcgctact ttgccttaaa caatcagatt catttgtaac aattttaaga
    18421 tgttcattaa atgacccttt tataagagaa aattcttgca actttcttga aaagtgacta
    18481 agatgagtct agaaaagctg aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt
    18541 cggggacaat gagccttggt ggttttttga agactgggaa gaagatattg aagaagaaga
    18601 aacattcgag tctttcgaag aagcacgaca agcgtacgaa aaacaatgga acgaaatcca
    18661 tcaaaattat gaatatatca atgcaaaaag taactttctc agcgcctttt ggaatgacgg
    18721 agatgagcgc tggtgtgaag agtgtgacga tgatttacag caatacaagg gacttgcgtt
    18781 gttgaaaaat catcagccca tcacagtaga aagcagaaag gaattttatg aaacaactaa
    18841 ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa
    18901 agcacaaaaa ttattatgaa ggacccaagg tgatcgttac ttgggccctt ggtcatttat
    18961 taggattgaa aatgccggaa gatctgaata aggaatggca gagctggcaa atggacacgt
    19021 tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc taaaacagga catcaattaa
    19081 aagcaatcaa gcagctggct cagcgaaaag atgtctcaga agcagtcatt gcgacagatg
    19141 ccggaagaga aggggaactt gttgctcgct ggatcttaga gtatgtgcat ttcaataaac
    19201 cagtgaagcg gttatggatc tcttctcaaa cagataaagc aatcaaaact ggattcaagc
    19261 aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag
    19321 cggactggct ggtaggattg aatgtgacca gagccttaac tgtgaagtac gcggataatt
    19381 tatcagcagg ccgtgtgcag acaccgacac tagcattagt gagagaccaa gaaaagaaaa
    19441 tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt gcaagctgga aaagaacaag
    19501 ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca agaagaagca caaaaacttg
    19561 ttcaattgat cagtacaagc aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg
    19621 aatctgcgcc attgccttat gatttaacag agatccaaag agaagccaat caacgctatg
    19681 gctattctgc aaagaaaaca ttaagtcttg tacaaagttt gtatgagacc cataagatcg
    19741 ttacttatcc gcgtacagac agcaaatatc tgacaaatga catgaaaagt acgatgaaag
    19801 aacgattgca agcagtcagc gactttgctc ctgaggtcaa gcagtatttg aaaaacggag
    19861 ccatcgtcca acagacaaaa gtattccaag acagtaaagt gaccgatcac catgcacttt
    19921 tgccaactga aaacagagca cgttatgaaa aattaagtaa tgaagagcga aaaatctatc
    19981 aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc ccataaagtc agccaaacaa
    20041 aagttacggt tgaatttggt aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag
    20101 caggatggaa aggcgaaact gaaactgaga cggaaacagt caaatgggaa aaaggaatga
    20161 gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc accgcctaaa ccattgtcag
    20221 aagcaagctt gcttggtcaa atggaaaaat acagtctggg aaccccagct acgcgtgcag
    20281 agattatcga gaaactaatc aaatctgaac tgatggaacg tacccctcaa ggactgcaag
    20341 tatctcctaa aggtaagcaa ttattagtat tggtaaatcc ctctttagta acaccagatt
    20401 tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc aggaaagaaa caagcaggaa
    20461 ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt gaatgagatc aaatcaagca
    20521 aacaagaata tcaagatttc tcgattaccc aaaagaaatg cccagaatgt ggggccaatc
    20581 tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg caccaattca gattgcaact
    20641 accgcagaag aaaagatcca aaagtctcta atcatcgatg cccgcaatgt caccggaaaa
    20701 tggaaatcat cgaaggtaaa aacggtgctt atttccgttg taaatacgat ggaacgacag
    20761 aaaaattgct tgataaaaaa gagcagaaga agaaaatgac caaacatgaa gaacgacgct
    20821 tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag cccattagca gcagcactaa
    20881 aagcagcaat gaagaagtaa taagatatga aagaggttgt ga
//
LOCUS       DAYYNQ010000050        20605 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000050 DAYYNQ010000000
VERSION     DAYYNQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.050"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1236
                     /locus_tag="ACHBJ1_001676"
     CDS             34..1236
                     /locus_tag="ACHBJ1_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM9430612.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            complement(1413..2681)
                     /locus_tag="ACHBJ1_001677"
     CDS             complement(1413..2681)
                     /locus_tag="ACHBJ1_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM9430613.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     regulatory      complement(2732..2799)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(2842..3489)
                     /locus_tag="ACHBJ1_001678"
     CDS             complement(2842..3489)
                     /locus_tag="ACHBJ1_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFM9430614.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            complement(3754..4257)
                     /locus_tag="ACHBJ1_001679"
     CDS             complement(3754..4257)
                     /locus_tag="ACHBJ1_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9430615.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            complement(4381..5145)
                     /locus_tag="ACHBJ1_001680"
     CDS             complement(4381..5145)
                     /locus_tag="ACHBJ1_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM9430616.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            5253..5528
                     /locus_tag="ACHBJ1_001681"
     CDS             5253..5528
                     /locus_tag="ACHBJ1_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM9430617.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            complement(5876..7027)
                     /locus_tag="ACHBJ1_001682"
     CDS             complement(5876..7027)
                     /locus_tag="ACHBJ1_001682"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9430618.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(7047..7769)
                     /locus_tag="ACHBJ1_001683"
     CDS             complement(7047..7769)
                     /locus_tag="ACHBJ1_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9430619.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            complement(7762..9213)
                     /locus_tag="ACHBJ1_001684"
     CDS             complement(7762..9213)
                     /locus_tag="ACHBJ1_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM9430620.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            9355..10437
                     /locus_tag="ACHBJ1_001685"
     CDS             9355..10437
                     /locus_tag="ACHBJ1_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9430621.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            10771..11709
                     /gene="yidC"
                     /locus_tag="ACHBJ1_001686"
     CDS             10771..11709
                     /gene="yidC"
                     /locus_tag="ACHBJ1_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM9430622.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11890..12294)
                     /locus_tag="ACHBJ1_001687"
     CDS             complement(11890..12294)
                     /locus_tag="ACHBJ1_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9430623.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12310..13119)
                     /locus_tag="ACHBJ1_001688"
     CDS             complement(12310..13119)
                     /locus_tag="ACHBJ1_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9430624.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(13106..13996)
                     /locus_tag="ACHBJ1_001689"
     CDS             complement(13106..13996)
                     /locus_tag="ACHBJ1_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9430625.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            complement(14009..14488)
                     /locus_tag="ACHBJ1_001690"
     CDS             complement(14009..14488)
                     /locus_tag="ACHBJ1_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9430626.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            complement(14505..16271)
                     /locus_tag="ACHBJ1_001691"
     CDS             complement(14505..16271)
                     /locus_tag="ACHBJ1_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFM9430627.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            complement(16317..17258)
                     /locus_tag="ACHBJ1_001692"
     CDS             complement(16317..17258)
                     /locus_tag="ACHBJ1_001692"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFM9430628.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            complement(17255..18250)
                     /gene="lacD"
                     /locus_tag="ACHBJ1_001693"
     CDS             complement(17255..18250)
                     /gene="lacD"
                     /locus_tag="ACHBJ1_001693"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFM9430629.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            complement(18243..19436)
                     /locus_tag="ACHBJ1_001694"
     CDS             complement(18243..19436)
                     /locus_tag="ACHBJ1_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9430630.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            complement(19454..20182)
                     /locus_tag="ACHBJ1_001695"
     CDS             complement(19454..20182)
                     /locus_tag="ACHBJ1_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9430631.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
BASE COUNT         5979 a         4444 c         3610 g         6572 t
ORIGIN      
        1 gaaaaaattt acaaatcgaa aggagttcat tcaatgaatc ttactagaga cctgatattt
       61 ttagttatct atattttagg aatgtcaggc atcctctatt ggcgcaagcg ccaaagcgat
      121 cccgctctat tcttttattg tgggacgtgg acttttatga tgatggtcac ttacttcgtc
      181 gttttagaag ttttttatca tacattttat tttttgattc cattgctatt tttcagtatt
      241 tttgcatttt tctattttaa ggaaaaacgt aggttaatca atggcgtact ttttaatacg
      301 tttttgattg tctttggtgg ttatctaggg tggctactaa tagagacgca aaatatcttt
      361 atcggtgggt tattggcttt gatcgggata ccaatattaa ttcttttggc aatcggtatc
      421 tacattttga tcattttttt attttggaat ggtgttgtag tcatgaaaaa agaatcccgt
      481 tctttggcca accttttgac tctcttgtta ggagtcgctt tgactatctt tttgatattg
      541 gactatacat tagtccattg gctcccacaa tgggcaaatg ctctatttgc tgtcattccg
      601 tttatttttg cttatctact catcgttttc tttaatttta tgactgtttc aattttgtat
      661 cagttcaacc gtccaaggta taaccaagat tatatcgttg ttctcggcgc tggcttgatt
      721 gatggtgaac gcgtatctcc cctacttgct aaaaggatca ataaggcgat cgcattttat
      781 tgggcacaaa gtaaagcaac tttgaatccg ccagtcttgc tcatgtcagg aggaaaagga
      841 accgatgaaa aagtagctga agccattgca atgaaacaat atgctttaga aaaagggatt
      901 cccgagcgtg atattttagt agaaacgaat tccacaacga cgctagaaaa tatgctgttt
      961 tcaaaagaaa taatggatca gcaaatgaat gggccttatc gcgcgatttt tacttcaaat
     1021 aattaccata tctttagagc tggactatat gcgcgacaag cgaaactcaa tgcaaacgga
     1081 atcggtgcaa aaactgcact atactactta ccgaatgcta tcttaagaga atttattgcc
     1141 atccttgtct tacacaaaaa acgccatctt atcatctgtg gcttaatgat tggctttgca
     1201 gtattttctg ctcttattct caaatttgct caataaaaaa gagcgtggga caaaactaaa
     1261 aatcagtttt gccacagact ctaactccga ataaacggtg ggagcagaag caactccttc
     1321 ggaaataagc gaaattcaca aaaatttaaa aagcaatttt cgtgaatttc ttcttatttc
     1381 tcggagttaa acacttctgt cccaacctct ttttatttct ctttttgtcg tgagattctt
     1441 ccaaggatcg gtaggaacaa gattcctttt ttcacttcaa ctttttgcgc agaatagatt
     1501 ccactatttc ctgaacacat aaagctaatc aaacagatca taaagaagta gctggctgct
     1561 tgacctccaa aaagttcgat ccccataaca aagcaagcaa tcggtgtatt cgttgctcca
     1621 gagaacaccc caataaaccc taatcctgct aaaaaaggta ctgatacatg aagaatgggt
     1681 gctaatgtcg cacctaaagt tgccccaatc tcaaacaaag gggtcacttc gcctccttgg
     1741 tatcccgcgc caagtgatag aacggtaaag ccaagtttcc caagaaaatc ataaggatga
     1801 gcacttccat taaatgcatc ttgtaataaa ggtaagctta atccgagata tctttgcgtc
     1861 tgtaaaatca ctactacgag taccacgact gctcctccaa taaagttgcg taaaactgga
     1921 tgggggatcc gctttgcata ccaagctttt aaccagacga tcgaacggct aaagagccag
     1981 ccaatgatac caaatgcaat gcccgcaagc aacaatttga agaaaagtga agggtcccat
     2041 gcaggaatcg cccccatttg ataatgggta tgcttgacat tgaaactttc cgttacaaag
     2101 ttcgcaaaaa gtgctgcaaa aaagcttgga aacagtgctt cggttctcac ttttccgatt
     2161 gccaatactt ctaatccaaa caatgtccca gccaagggtg tcccaaatac tgagctaaaa
     2221 cctgcgctga tcccactgat gacaagaatc tcacgctcga ttttgttcaa ttttagccaa
     2281 cgagcaaaat tctcagcaag tgccccgccc atttggaccg ctgtaccctc ccgaccaaca
     2341 gaaccaccaa aaagatgcgt ggtaatggtg ccaaataagg tcagtgggat cagccgtaat
     2401 ggaatctttt catttcctcc attcccttga tcgatcacta aattgtttcc ccgggaagaa
     2461 ttgcccccat acgtcgtata tagataagca aaaagtcccc cacttatcgg caaaagaaaa
     2521 agtaaccaag gatgctccag ccgaatagct gtcacatcag tcaaactagt tagaaagaat
     2581 gcggataaac ttcccataaa aatacctaaa attgctgtga tcaataacca tttgatcata
     2641 tagatgatca ccttcaatgg atcaggtaat cgattttcca tctcatttcc tcctcaaaaa
     2701 aacgcctact aaaaaaaggg gtagccccgt ttctttagta ggcgtcatta gttatcttac
     2761 gatcaacgtt taaaggcgaa caccatcacc tattcacttc accaaataag atagcatatt
     2821 tttcagaaaa atacaatgaa atcaccgctc acgtttcact cgttctgtga attcgtaaaa
     2881 aggagctaat tgtaccaact gctgattaca ttggtcacta tcatgacaaa tataatttcc
     2941 ttttttcgtg tatgttccat ctgaaccagt ttttgtggta gaaagaaaga gtgaaacatt
     3001 tgaagttctt tgacaaatcg aacaaattcc tttgttgatt gtaggagata atgttccact
     3061 gatccctttt agtttttctt cttcataata gatcataaat tttttttggc tacctggatc
     3121 attccaacca atataagtat gttcccttaa atccagttga ttccaatctg gttgttttaa
     3181 ctttttcact tttcggaata atttatcgat ctgcggtttt gtcggttgta tgaatggaag
     3241 aacgatcact ttcaattcct ctaatagtcg ttctgctttt tcttttttta gagtgacgtc
     3301 tagtaccttt tccataaatt catttacagc tgtattagga tgaccaagta cctcgtggat
     3361 cttttccgca gctaatgctt ggactgtttg gactgtcttt ggatcattca ctgaactata
     3421 ggcgcttaac agctgctcga cttctcgctt gataaaataa tattgatatg gtttgattgt
     3481 tttttccatg actttgagtc ctttcttctt tcaaaatcgg ctactttgaa gaaaaaagtg
     3541 atctttctat caaagtgcct ttagataaag ccctcaaact cttttaaata agatacacat
     3601 cgcatcttat catcaccccc tttattttta tgttggttat gtactttttc atctcaacaa
     3661 agtaaacact aaaaagacca attagtcaat gtctaaacaa aaaacccgag gatttttttc
     3721 aaaatactcg ggtcaaatac ttagttaact accttaaatc gattgttctt taccgaaata
     3781 tcgttgccat ttttgtttca ttgaagctgt cattggttga gctgcttctt tgactgcaca
     3841 atatttatcc acaaaagtaa cgatataacc ttctttatat ttaggagggg caatcgttgc
     3901 tccccacata tgtttcaaaa taatatcacg ctctagatct gaaagctccg tgattttttc
     3961 agcatttttc acagcgatcc ttgggtgaac atacgcatgt gtaccttcat cgaacttcgt
     4021 cgtacgccaa tcgtagtaaa ataaatcatg taaaaggcct gctcgagcag tcgctcttgc
     4081 atcaccgccc cattttttcg ctaatttgta gctatagtaa gacacactga ttgaatgctc
     4141 taatcgtgtc gagtggacat gttgcgtgta atttgctaat ttctttactg catcagtttc
     4201 taatagatca tttatataag acatatattc ttcgtcttca cgccattttt ctgtcataat
     4261 tatcgatctg ccaccttttt gaattccatt tatctttgaa attgaactta gtataccact
     4321 agtgttcgta atattctagt gaggttttcg caaaatgcag aaagcttaat agttccttaa
     4381 ctatacggag aagtgataaa gcatgatgcc cgctgcaact cctacattta aagactccgc
     4441 atttccttga atcggtatat acacattttt atccgttagc gccagcactt ctggacgtac
     4501 gccttgacct tcattcccca tcacgatcgc ataattttct gttgtctgga tatcattcag
     4561 ggacactgct tcttcattca gttctgttcc ataggttata aatccttgct ctttaaggat
     4621 aggcaatagt tttgttagat cctcttggat gatcggtaaa tgatagttac tcccctgcat
     4681 gcttcttaat actttggtac tgtagacatc cgccgttccc ttacctagta caacacaaga
     4741 tagaccaaaa gcatcggctg tgcggatcat tgtccctaca ttgccgggat cttgtacgtt
     4801 gtcgagcaac aaccaaccac cttcaaaatt cggtatctct cttttttcag cttccaccac
     4861 tgccatgatc ccttgcggag ttggtagatc tgaaacagtt gccatcactt cttctgatac
     4921 tagtgtaaca gaaagcgcct gattttcttc aagccaacta gcccactcag tcagaccgcg
     4981 ttgcgtaata aaaagttcgt tgaccttcgc atttgaggca acagcctcct cgatcaggtg
     5041 aaacccttca agcagatatt cccctttttc ttcacgatat tttttcttat gtaatttttt
     5101 taattctttg attcttggat tttttgctga ttggatctct ttcacatttt tcacctcaac
     5161 tgattatacc atggattaga tcattgcact tgttcagtag caaaaaagtc ggtatcatta
     5221 atttaaaaat aaaagaaaga acggtgaata aaatgaacaa aatcaagatg aacgtgcaag
     5281 gacgtgtaca aagcgttggt tttcgctata tgacaaaaat cgtagcggat caattaggga
     5341 tcaccggaac agtgaaaaat gaagatgacg gctctgtcac gatcgaagct gcgggcgacg
     5401 atgaggcaat caaaaaattc gtggaagaag tcaaacgatc accaagcccc agcggacgtg
     5461 tccaatatgt agaccttcag gaaaatccga tgatggagga cagaaaaaag tttgttgtaa
     5521 taggctgacc ttctagaaat gaataggaaa tgtcagaaaa atcggactgt ctgtatgtag
     5581 atcgaaaaag tttgcccctc tctccatcct agatcgacaa aaaaacattc ttattagcga
     5641 gtttagtaac cagtgatagg catacgtaaa agcgggagga cttcaaaaat tttggttcaa
     5701 taaaatggag tctataatat tttatggtag tggaaaatcg actttgattt tccactacca
     5761 ctttttcatt gtcaaatcct gacgctgccc agtcaacaag ccaactctcg aaaggatcat
     5821 tttccctctt tgttctaatg gatacccata gctgttttca aatatacagt tttatctaaa
     5881 aatttagttt tgctaaacga tcaaaggctt cacctaagat ttccaatggc tgagtcgctg
     5941 ctagtcgaat atagttgtcc ccgatcgttt ccccaaacgc ttttccaggg acaaaaagta
     6001 cgccgctttt ttctaaaacc tcttccacaa acgttactga atcgagccca ctcttactga
     6061 tattaataaa agcatacatg cttccagaaa cagaagataa agataagtaa gggatttctg
     6121 ccactctttt ctcaatataa gctaatcgtt ctttgaatac ttcaacaact ggcggtacaa
     6181 gctgttcata gtgctttaag gcgtagattg cacctctttg agaagcagtc ggtgcggaat
     6241 aagtgatgtt ttcattgatc aacccagcaa tttgattgat gtatgctggt gcgatcatgt
     6301 accctaagcg ccagccagtc atcgcaaacc ccttcgatac accactgaca gtgatcgtat
     6361 tatcgggcgc aaatcgcgcc atcggtacga acttctcttt aaaggcaaag tcttcataaa
     6421 tctcatcgga aatgatatag agttcatttt cgattgcgac ttcagcgatt tttttcaacg
     6481 tgctttcaga aaaaacagct cccgtaggat tattcggcga attgatgacg atcacctttg
     6541 tacgatccgt aatcgctgct tttaaatctt ccacatccaa ctggaagccc ttttcttcat
     6601 aggtaggaac aaaaacaggc ttgcctccag ataaaatgac ttgttcccga tatggtgaaa
     6661 aataaggttc atggatgatg acctcatcct ctggatctag taatgcctgt aaaactagat
     6721 acatcccgtg cattgctccg actgtagctc gaacttggtt gcattcaacg ctcaatccat
     6781 atttcttttg ataaaaatct acgaccgttg acaagaattc tttgctgcca tcaggcgcag
     6841 tataattagt agcaccattt tttacatctg tataagctgc ttcaataatc gtcggatcag
     6901 tgatcaagtc aggatcacca atcgataaat cgataatatc ctctttttcc tttgccaaaa
     6961 tagcaatatt catcaacaga ttttctgctg gttgctgaaa ccggcgtgca atcttacttt
     7021 tgtccatctt acttcctcca tctttgttac aacactttgt tcaagaaatc ctgtgttcga
     7081 ctattttgcg gattttcaaa aatctctttc ggtgttccct cttcttgaat gatgccgtca
     7141 gccataaaaa tcacacgatc ggcaacttct ttagcaaaac ccatttcatg agtgaccacg
     7201 accatcgtca tccctttttt agctaaatcc tgcatgactg ccaacacttc accgaccatt
     7261 tcaggatcaa gtgcagatgt aggttcatcg aaaagcatca catcaggatt cattgctaat
     7321 gctcgagcaa ttgctacccg ttgttgctgt cccccagata aactggtagg ataactgtcc
     7381 actttttcct ttagaccaac ttggttcaat aatgatagag catgttcttt tgctttttta
     7441 gggtcttctt ttttgatttt tacaggtgtg atggtcaaat tttcgagtac attcatatgg
     7501 ggaaacaaat taaaactttg aaataccatt cccatttttt gacgaatttg attgatatcg
     7561 ctgtcagggg ctgtaatgtt ttgtccctta aaaaagattt caccagaagt tggttcttcc
     7621 agcagattca tacaacgtag aattgtactt ttcccacttc ctgaagggcc aatgatcacc
     7681 actacttctc cttctttcac agaaagatca attgatttta agacttcatt ctcgccaaaa
     7741 ctttttgaca gtttttttac attaagcatt cagacccatt ctcctttcaa ctaaagctaa
     7801 tatacgggaa atactgaagg tcaacacgaa ataaatcaaa gagaccaaca catatggtag
     7861 gaatggtttg aagctagcac cttgtaccac gcccgtttga aagatcaatt ccgatacccc
     7921 aatgaccgaa acaactgagg actctttgat gatcgtgaca aactcatttc ctagagctgg
     7981 aagaatattt ttgattgctt gtgggaagat gatgaaacgc atggcttctt tctggtccat
     8041 tcctaaagaa cgtgcagctt ccatctgtcc tttgttgacc gcattgatcc cggcacggac
     8101 aatttcggct acataagcag cactattcaa cgagacagca atacaacctg ctaccagttt
     8161 cgatagatcc aatcccagta ctcctgttcc gaagtaaacg ataaagattt gaactaagag
     8221 aggtgttccc cgtacgtatt cgatataaat aatggctaat gcctttaaaa atttattctt
     8281 acccaatttc atcagtgcaa gcaaactacc aaagaccatt ccaaacaata cacccacaaa
     8341 agctaataaa atagtgtatt ttgttccaat caaataatac ggaccgtatt tttgaagaaa
     8401 ggactgatca tcttggaaca tcaattgatt cgcttgtttt tgataatctt tcagcaagcc
     8461 gtcgtcattg atcgttttga ttgacgcatt gacatgattt ttcagttctg gtgtattttt
     8521 tgacatcgca atagctgtgg ttttttcgcc ttgcgcaaat tttactggag tcaaagctac
     8581 tttcttattc tgttgggcat atgcttctgc aactggttgt tccagcacaa caccttcaac
     8641 tttttcattc agcaaattat tgatcagatc aggaactttt tgcagggagg tcaactctgc
     8701 accagtcagt tcatttttca ctaactcctc ttgtgtggtt tgcttttgcg ccccaacggc
     8761 taagccagaa aagtcggctg tcgtcttaaa tttatttttg tcctctttac gaacgagtac
     8821 tttttgctgt accacgaaat atgggtcaga aaagtcaact tcttgtaaac gttcttcagt
     8881 caccgccata ccagatataa ctaaatcgat cttgccagtt ttcaacgcac ctaataatgc
     8941 atcaaaacca agttcttcta cttttagttt cacacccata tcctttgcga tcttttcggc
     9001 aatggataca tcaaaaccaa cgattttatc cttaccatcc acattcgcat ggaattcata
     9061 gggcgcatag tctgcagata ggccaacgac taattctcca ctttgtttta tccgctcgta
     9121 aatttcatct ttttctgcac cttgtacttt tactggtaaa aaaaccagta gaaacatcag
     9181 cgccactaat aatatgttta ttcctttttt catccatttg gccacctttg aataataatg
     9241 taatcagaaa agctataaaa gaataaatat tcataaataa cttgctaata tactagcata
     9301 actattataa taaaaaaaga ttaatttaaa aaaataatgg aagaagtttt ttatatgaca
     9361 aaaaatttaa ttgttcgcgg tgcccctcaa gagtatgaaa taaaagaaaa cgcatgggat
     9421 tcactccctg aacaccttta caaaaggcat attgacaacg ttttgattct ccatggaaaa
     9481 acatcttgga aagcagtaaa agatgttttt ccagatttat caataacaaa aatttttgaa
     9541 tattatggtg gagaatgtac cgatccactt gctgacagtt ttgtttcttt atgcaaaaag
     9601 aaggatttca atggtattat tgcagtcggc ggcgggaaag tagcagatct aggaaaattg
     9661 attgcctata agacaaactt accaatcatc atcttaccta ccttagctgc gacgtgcgca
     9721 gcttatactc cacttagtgt ggtctataat gaagaaggag tgatgcttcg ctatgatatg
     9781 ttccctaaag caaatgattt agtgttgatc gatccaaaga tcattttgca ctcccctcgc
     9841 gagatgatga ttgccggtat tggtgacacc ttagcaaaat ggtatgaatc tcacgcaatc
     9901 atctctcagt taactatcaa gcctatcgaa gtccaagtag cggaatttgc agcgttacga
     9961 tgccgcgaaa tccttttaga gaaaagtgca gaggcaatgg aagcgatgga ccagcaaaaa
    10021 atcaatcaag cttttatcga tgtcgtggaa acaaacattt tattggctgg tatggttggt
    10081 gggtttggcg atgactacgg ccgaacagct ggagctcatt ccattcacga tgcgatgacc
    10141 gtactccctg atagccatca gcaacttcat ggaaacaaag ttgcatacgg tatccttgtc
    10201 cagctgatga tcgaaaataa attagcagaa atcgaacacc tactgccgtt ttatcatcac
    10261 ttgaacttac caacgagttt agctgatatg ggtttacatt taacagaaag tgactatcaa
    10321 gccgtagctg aacgtgcatg tttacctgat gaaatgatcc acttcatgaa agaaacgatc
    10381 acaccagcac tggttgtcca gaaaatgaaa gagttagaac aagtgacaca gggatagtta
    10441 gccccttata ccatggcagt cagctttttt atggatcggc tcaatggtaa aaaaaaagaa
    10501 gtggaaaaaa ttgacattcc acttcttttt tttgcgctaa aattatgatt tcaaggaaat
    10561 atccgttgct catcttattt tagagaacgg aggtgctaag tatgctttat actgaagtat
    10621 ttttagctgg attcttcttt ttaacattaa gcgtcttttt tgatcctcca atcggttatt
    10681 tctatgaaat ttctatgaag ttgattgaat tatgagcgct tttttagtat agttaagctt
    10741 gtgtaaaaat tataaaagag gaagtatttt atgaataaat ggaaaaattg gttattgggc
    10801 tctgggcttg tcacgatcat gcttttcttg tccggttgtg tccgcatgga ttcaaatggt
    10861 aaccctgaca cttcaggaat catttaccgt atattggttc acccaatggg acaagcgatt
    10921 acatatttag tggaaaactt taattggtcc tacggctggg ctgttatctt gatgacagtc
    10981 atcgttcgta tcatcatttt gccattaggt attagtcaat cgaaaaaaac aatgatccaa
    11041 tctgaaaaga tgcaagcatt gaaaccacaa gttgaagcta cgcaacaaaa attgaaagct
    11101 gcgacaactc gagaagaaca aatggctgca caagctgaaa tgcaacaagt ttatcgtgaa
    11161 aatggcttaa gtatgactgg tggtatcggt tgtttaccgc ttctgatcca aatgccgatc
    11221 ttttctgcgc tatatttcac tgctcgctat acagaaggca tccgtgaatc aaccttttac
    11281 gggatcgacc taggtaaccc tagcttagtt ttagtagcga ttgccggtat tgcttattta
    11341 cttcaaggct atatctcaac tatcggtatc cctgaagaac aaaagaaaac gatgcgtacg
    11401 atgttgatcg tatcacccgc tatgatcgtc ttcatgtcga tcagtgcccc agctggtgtc
    11461 actctttact gggtagtcgg tgggatcttt agttgtctac aaacattcat tacgaatgtc
    11521 atcatgaaac caagattaaa agcgcaagtt gcggaagaat taaaacaaaa tccgccaaaa
    11581 caagtggtga cgcctcgtaa agatgtcaca cctgctgaac ctgcgaagca aacaaaaaaa
    11641 caagtacaag caccaaaaaa caatgcgaag aatggtcgga atgctggtaa acagcaaaag
    11701 cgtaaatagt tatttgtaac tagcttcttc gatcgaccca gatcatttct gattttatga
    11761 cgataaaagc caacgtactg tgaaatatag aaaactatat ttcgcggtac gttggctttt
    11821 ttatacacgg cctctttata catccgtatt tatatccagt tgtatcacca gtcaagaagt
    11881 tgtgcttgat tattcttctt catcgtctcc catgccagca atcacttggt tcactgaaac
    11941 gatactctca cgacccgaac caataaaatc tggttctgct ccaatcattt gcgaattgat
    12001 gaattcgatg accatcggca aattcatgcc agcatatagt tcgatcgttt caccttccat
    12061 gattagcttg gaaacggtat ttgcaggtgt cccacccaac agatcgcaaa gcactacgta
    12121 atctttcaat ggtgctgtaa cggagaggaa tctttcttta tacgtgtccg cgccatcttc
    12181 aggcaataaa gggatcgtat agatattttc ttgtggtccc atgatcattt ctgtactgtt
    12241 ttttagttct tcacaaaatt taccatgact aatcaagact agttgtctac tcatctttcc
    12301 aattctccct tatttcgaaa tcacacctaa tgcagataat gctacacata cgatcaatac
    12361 gatgaatatt gctcgagtag gtgtcatatt tttcttacct aacatccaat aaacgccacc
    12421 aacgattcca gctggtaaaa gtttaggtaa gatcatatcg atatagtttt gcatattcaa
    12481 cgttacatcc ccaatcgttg gtgcccatga gaatttaaca ttgatcatgg ttgctactaa
    12541 cgctccaacc atgaaaactc ctagtaaagt agccgcatct gtcaatgcag tcaagcgtga
    12601 ttgcatcgtg gtaactagag aaatcccttc tttgtaggca aattctagtt gtttccaacg
    12661 aaagaccatg acagcgacct gagcaaggat ccagataagg atacctactg gattaccatc
    12721 tgttgccata tttgcggcta aagctccaaa tatggtcggg atcaatgccg caaatacaga
    12781 atcaccaatt gcagcgaagg aacccatcaa tcctgctttc atcccagaaa cagtttcctt
    12841 tgaagagact ccttcttttt cttcgatagc aagatcgatc ccagtaatga tcgtgttaaa
    12901 gaaattagac gtattaaaaa attgcgtatg tgttttcatc atttctttta attctggtgt
    12961 atcatcacca tagatttttc taagttgtgg caagatagta tacagataac ctgaaccttg
    13021 cattctttca tagttccaac ctaattggaa agtaaacaaa cttctacgat tgatctgctt
    13081 aaaatcttct ttggtcaatt tataattaga tttcgtcatc ttcgatttcc ccctcatcag
    13141 attgtgattg ctgacttact actggatgtt ggtcattctt ttgatcattc ttataactga
    13201 tcgttgccag tgctagacca atcaatgcca cagcaagcat cggtaatgaa ttgaatgttc
    13261 ctgggaaatc tttgaccacg ctagccacac ttgttcctaa caattggaca ttcgagaaca
    13321 ctgttgctaa taacgctgtg atcgtaaatc ctaaaatcag ataagaaatg tgtttcttta
    13381 ctggtaggta gcgtagtaaa attgcaaacc caactgctgg taagactgcc cctgcaacag
    13441 ataagccatc tcctagccat tttaggtcgc cgtttaaaac agccacaaca ttttggacta
    13501 agccaccacc gaatgctagg gccaagaata caggaaccat tctggaaagt gcccaaggta
    13561 atgcccccat caagaagttc cgttcaattg ctttgtaatt cattgtttcc acatgttgat
    13621 cgatccgatg tgcaaaatac gtattcgcaa aacgtgccaa aatatctaat tggatcatca
    13681 aacttgctac aggaaccgcg atggccgcaa tcgcttgttc tgggttcatc cctagagaaa
    13741 tggagaaagc tgttgctaag atcgttccag agttggcatc gatttttgat gcaccaccaa
    13801 atgtccctac ccctaaaaca gtcaactgca tacttccacc aatgattaaa cctgctttta
    13861 aatcacccat tactaaccca gcatataatc ctgcaaaaac tggggcgctc attgaagaat
    13921 aaatctgtaa ttcatctaaa atttgatacc ctgcatataa tgttaataat aatatctgcc
    13981 accattgaat atccatgaat cattcctcct atttgattaa aagactcata aagtcttctg
    14041 ctttgtcatt tggcaccatt tgagcgatca aatgaacgcc aagctgatct aatttcttga
    14101 agacctctac atccgcatct acgacattga ttgattttgt gatggaacgt gtttcatctg
    14161 tttgtgacat attgccgaca tttaattctt tgatcggtac acctttttct actagcgcta
    14221 agatacgatc tggttttttc gctacgatca gcactcgttg agaatcatat ttacctgcta
    14281 aaatatttgc ggctgctttt tcaataggta aaatacttaa tttgactcca gctggcgttg
    14341 ccaactttaa cccacttttt tcgatcgcat ttgttgctac ctcatcgtct acgaccatga
    14401 tccgaccaat attcaacttt gtcgtccaaa gatttgccac ttgtccatgt atcaaacgtc
    14461 catcaattct tactccaata attgccatca tccattctcc ttttctaact tttttctatt
    14521 ggttctttga caaaatctat aacttcactg aatctacctt ctgtttcaaa gataacgatt
    14581 tcattttgtc cttttctaaa taaaccctcg ggaacaaaca gtgaaagaat tggtcctact
    14641 tcccaaaaac gaccaatatt cgtgccattc acccaaacga tccctttccc aaactcacta
    14701 aggtccaaat aagtatcacc gatttctgac agttccgctt caaagcggta aaatgctggt
    14761 tgacctgctt cccatttttt tgaaaaatcg atgtgttctg tagtctctaa aggtaaacaa
    14821 tactgtgtcc attctgtgat aaaatgaaga tcgctcatca ctcctgtacg tattcctttt
    14881 ctttgcgtat cagctagcag cttgtgtcca tagttgaccc gtcccatatt ttcaaataat
    14941 agatcaattt gatggtcttc tttctcaagt gtcaatggaa tggtctcacc gatctctgtt
    15001 tggtattgaa tcgcttgctt ttcttcatcg agaaaaacat ggacccgatc acgtccgtcg
    15061 atgatccgta atttttcatt gaccgcatct tttttgatcg ttgttcgata caataggtac
    15121 ccagtattct gacccaattc ttccattgtt tgaggatagg tcgagtggat cggcttgctg
    15181 atggtatcca gtgttgcgaa aaagctgact ttttctttca agttgattcc ttttttgacg
    15241 attgtttctt ttatccatgg ttccatttgc tgcatttctg gaaaattttc tgccatcatt
    15301 cgtttcagag caaaatattt ttccgttgga tttccctgct catccaatgg tgcatcgtaa
    15361 tcataggaag tgacttgcgg caaatcaatc gcgcctctgg ctgaacagcc attcataaac
    15421 ccaaaattgg ttccgccatg gaacatatag agattgatac tgccgatctg taatgcttct
    15481 ttgactgcat ccgctaattc ttccggatct cttttaatga tcggctcttt ccaacgatta
    15541 aaccagccat cccaaaattc catacacatc aacggccatt ttttaccatg tgcttcaaaa
    15601 aactgggcca tatctgcaaa attttctttt gcttttgagc caaaattccc tgtcgtaaaa
    15661 acatcatcgt caatcaaaat gcctgctctt aatgtggctt gccaaggtcc atcagaagtg
    15721 aatagcggta catcgattcc accttcctcc aagtattttt ttacttggcg caggtatgct
    15781 ttctcttcac catacgaacc gtactcattt tctacctgca ccattaagac gttgccacca
    15841 tgagtgacta gatgaggaac gattttaggt agtagcgttt ggtaataccg tttgacatac
    15901 ttcatgaagc gaggatccgc tgaacgaata cggcactcct ttgtcaatag ccaagcaggc
    15961 aagccaccaa attcccattc tgcacaaata taaggggctg gacgtacgat tgcgtataat
    16021 ccaagggatt gcgctaactc tagaaaatgg gaaagatcca atattttcga aaaatcatag
    16081 gttccttctt ctttttcatg gagattccaa ggcacatagg tttccacagt attaaatcca
    16141 agcgctttca aattgtgcaa tgaccgctcc caattcgtcg gatgcactcg gaaataatgg
    16201 atggcaccag ataaaatttt gaaaggttga ccgtctaaca aaaactcttc ttttatttca
    16261 aatctagtca agcaaatttc ctcctcgttt ttagaaaccg ctttctaatt ttataatcaa
    16321 cgaatctttc tgacgttgat ttccccccta aaataatcga ctttcacagg atcgatccat
    16381 cctgtttttt cttgttgtgc attcgccatc ccacaggcca tcgcaaacgc aagtgtttct
    16441 tcaatcgtat agctctttga tagagcatgt gctaagcctg caatagtcga atctcctgac
    16501 cccacaggat tcaccaccgc gattttagga atcgtaacct gatagaagtg tctccctact
    16561 ttagcgattg ctccttttgc gcctagcgaa agaacgatcc aattgattcc ttcaaaaagc
    16621 ggatgatcca ataatgattg gatttcatct aacgaagtag ccgaagaaat tgtttggtgg
    16681 accaactctc ctaattcttc caaattaggt ttgatcaaaa atggtttttc cttgccttcc
    16741 aagacttcaa gcaaggtttg accagaagta tctaacaata ctcgtgttcc ttttttattc
    16801 gttagggcaa gtaactctgc ataataactt ttagaaaatc cttgaggcag actacccgaa
    16861 atcgtaaaga tatcagttgt ttccattagt cgtgcaaaat gctcggtaaa tttatgttgc
    16921 tcttcttccg tcacaacagg accgctttcc aatagttctg tttgattcac tccatgtaaa
    16981 atagcaatcg aatctctcgt ttcccctttg ataggtaaaa attgatgcgt tatccctgat
    17041 tgatcgagtt gttctttgat atatgctcca tgctgcccgc cgactaatcc agtagctaaa
    17101 acatcatctg ctaataaatg aaggacacga gcaacattca agccttttcc tccagccgtt
    17161 ttgttgaccg ttttgacccg attgacttgg tcgatccgta attcatccaa cgtgtaagcg
    17221 atgtcaattg aaggattcat ggtaactgtc gtgatcattt tgctgtcacc gaatcttctt
    17281 ttactttgtc aaaccaaggg gtggcgtttt catttatcac tttattcaac gattcgatat
    17341 tttgtcgccc ttgtgttctc aaccactcaa tcgcaccttc tctaccattt tcaacatatg
    17401 ctttgactcc atctttccaa gtcgcacgtc cgcataacac accgttgaat gttgaaccag
    17461 attcatgagc aaagatcaat gtttgttgga acaatgcagt cgaaacccct gcactcaaga
    17521 agataaatgg taacggtgtt gcatcacttt gttccttgaa gaatgccgct gcttcttttt
    17581 tactatacgc aatttcacca tcagaataac cttctacata gttcatattg actggcactt
    17641 ctactttcaa tacatccact ttatatttag gatcagaaaa ttctttcatc atctcgatca
    17701 ctttatgcgg tttgatcttc gcatattcta atgaagtgct gtcattggtt ttggcatcat
    17761 acgaaaccaa ttctagataa aacggacgct tttcacctgc acattcactg cctaaacgtt
    17821 cgataaatac atgtttcaaa tgattgatct ctgggtcttc atctacatca taataaagca
    17881 aaaacttgat cgcatcggct gtttgttcct ttaatctaag cgtagaccaa tccgctaata
    17941 aatcaggcag acgtcctgga gttgtcgcat cataacccgt tttttcatag gctaaaagta
    18001 gtcctgcttc ctgatgtctg gctttagcag ctggcaatcc atactctgga tctagtaaaa
    18061 tagcagaagc ataaggcgtt aattctgctg atactaattc tttgaattca gtgatctgtt
    18121 gcgccgttgg ttcttttcct aacgctttga tcatcttttt caaagcaccg cgttgatcaa
    18181 ttgccaatgc actgatgatt ccttcttttg ttgataaact cttcatagct gcaatttttt
    18241 cttcattcat ccttcgtttc ccctttgttc acatattcat ggataatgac acctttgacc
    18301 actcgattga ctgtacctgt aggcgatggt gtatctggtt tattcgctac ttttatcgac
    18361 gtatcgagcg caatgatttg ggctgtcatg atcataggta aggcaagata cgcatcaggt
    18421 aggctgatcg aagcttctaa caaaagactt gtacctgtga aatcgatgtt tcctggttgg
    18481 gcgatcgcga tgacactcgt ggtaatttgg tcatctttta tttcttctag aatatctaag
    18541 tcataatttc ttgtatacgg ttggttatta acaaaaccaa taacaagtgt ttcgctattt
    18601 aaaaacgatt ttggtccatg gcggaagccc atagaagaat caaagatcgt tgccacttta
    18661 ccggctgtca attccaatat tttcaattgt gcttctctcg ttgcaccagc caaacttcct
    18721 gaaccaagat aggcgatacg ttcaaatgac ttaggcaata atttttggat ttcttgatct
    18781 cgtgaaatta gatcttctcc agttctgatc aattgttgaa gataactttc ttttttctca
    18841 acggcagttt ggtcgaatac ccataacgca ctcaacaaca tgcacgtaaa acttcctgtc
    18901 atggcaaatc ctgcatcatt tgatttttct ggcatcagta aaagataatt tcgttcatct
    18961 gtcgctgcat tctttgctaa ctgaccttct ttagcacaag taatcgtcaa atgaaacaat
    19021 gtcttgattt gttcattggc aatcttgact gttgccacac tttctggact atttccacta
    19081 cgtgcaaatg acaccagtaa tgtcggttca tccggaaata gaaattcttt tggtgaagac
    19141 acgatatctg tcgtaccaaa gctatgaaaa ataaagtgat cgttatcacc gtattcattt
    19201 aaataaggta ctgctgtatc cccgacatat tgtgatgtgc ctgcaccagt aaaaatcact
    19261 cgaactcttt tgccatttgc tgcttttttt atctggtcaa aaaacaatgc taatttttct
    19321 tgattctgtg ctaatatctc agctgtttct ttccataatt gcggttgctg tttgatttct
    19381 cgcgtagtta tttccgcacc caactctttt aatttatcct tcgatagttc aaacatgttg
    19441 ttttccctct caatcatttt cggacgtggc ggatcttata atgaaactgg tctgctctcg
    19501 ctacactatc cgtatattca ataatttcat ttttcacatt ataggtcgtt cggcttagat
    19561 ataataccgg ggcaccatca gcgatacgaa gcggatcagc atctttcttt tgaacgatac
    19621 ttgcataaaa ttcttcatct gctaattgga cttgctgttc ataatcctga gaaaacacat
    19681 catacaatgg cttactttca agtaatggca gatcaagacc aataaatagt tttgcaggta
    19741 aataagaacg ttccaccatc aatggttcgt catccgctaa acgaagtctt ttcaatttga
    19801 aaactgtttc gcctaaattg atgtttagat tcttggcaac ctttttgttt gcttccattt
    19861 gcttgaattc caagatgact gtattaggca cccgtcccaa ttccctcatc tcttctgtaa
    19921 aactatacgc tcccgccaga tcagacgact tatctgatag atcagacaca tatgttcctt
    19981 ttccgtgtcg gcgataaata tacccttgcg tctctaattc ttgcaaagca aggcgaaccg
    20041 ttgtccgact cactccataa tgctgacaaa gttctctttc agataacaat ttgtcatgag
    20101 gtgtcatctg tgtttcgata cgttctttca atacgtcaac tagttgataa tataatgatt
    20161 ttcctttact tgagctctcc ataaaaatac ctcttcttta ctggttataa ccacaacaca
    20221 agaataccca catattcctc atttgtcaaa gggtttttgg cgtaaaaaag ctattataac
    20281 aaggtttcaa ggtaatttag aactggtatc aaaagatacc tcaaaaatac cagttgacaa
    20341 ataaaaaaca aaaaaaagct gaatcttggc aaagattcag ctaaatggtc tagagaagta
    20401 aagaaaaaat attctttata catctgtggc tatttctact atcgacaagc aaatgggcct
    20461 aatagactac tatgtaaata aaaatggctt tctctaaata aagtaggatt ttttattttt
    20521 tagataactt ttctctcatg ccggtctgat agtcgaaatt ttgttgtaca tttttaaaaa
    20581 taacaaaaat tttttatgat aattc
//
LOCUS       DAYYNQ010000051        20426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000051 DAYYNQ010000000
VERSION     DAYYNQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.051"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHBJ1_001696"
     CDS             214..498
                     /locus_tag="ACHBJ1_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM9430632.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACHBJ1_001697"
     CDS             complement(815..1198)
                     /locus_tag="ACHBJ1_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM9430633.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACHBJ1_001698"
     CDS             complement(1200..1784)
                     /locus_tag="ACHBJ1_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM9430634.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHBJ1_001699"
     CDS             complement(1831..3306)
                     /locus_tag="ACHBJ1_001699"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM9430635.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHBJ1_001700"
     CDS             3487..3798
                     /locus_tag="ACHBJ1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430636.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHBJ1_001701"
     CDS             complement(3913..6249)
                     /locus_tag="ACHBJ1_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFM9430637.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHBJ1_001702"
     CDS             6391..7059
                     /locus_tag="ACHBJ1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9430638.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHBJ1_001703"
     CDS             7062..8105
                     /locus_tag="ACHBJ1_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430639.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHBJ1_001704"
     CDS             complement(8342..9223)
                     /locus_tag="ACHBJ1_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9430640.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHBJ1_001705"
     CDS             9382..10110
                     /locus_tag="ACHBJ1_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM9430641.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHBJ1_001706"
     CDS             10266..10583
                     /locus_tag="ACHBJ1_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9430642.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHBJ1_001707"
     CDS             10576..11184
                     /locus_tag="ACHBJ1_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM9430643.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHBJ1_001708"
     CDS             11239..12030
                     /locus_tag="ACHBJ1_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM9430644.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACHBJ1_001709"
     CDS             12168..12656
                     /locus_tag="ACHBJ1_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM9430645.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACHBJ1_001710"
     CDS             12707..13579
                     /locus_tag="ACHBJ1_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM9430646.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACHBJ1_001711"
     CDS             13755..14117
                     /locus_tag="ACHBJ1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9430647.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACHBJ1_001712"
     CDS             14126..14878
                     /locus_tag="ACHBJ1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430648.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACHBJ1_001713"
     CDS             14892..16694
                     /locus_tag="ACHBJ1_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430649.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACHBJ1_001714"
     CDS             16971..18011
                     /locus_tag="ACHBJ1_001714"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM9430650.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACHBJ1_001715"
     CDS             complement(18063..20207)
                     /locus_tag="ACHBJ1_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9430651.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
BASE COUNT         6257 a         3587 c         4226 g         6356 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtg
//
LOCUS       DAYYNQ010000052        20181 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000052 DAYYNQ010000000
VERSION     DAYYNQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.052"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..1129
                     /locus_tag="ACHBJ1_001716"
     CDS             272..1129
                     /locus_tag="ACHBJ1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM9430652.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1244..2164
                     /gene="pstC"
                     /locus_tag="ACHBJ1_001717"
     CDS             1244..2164
                     /gene="pstC"
                     /locus_tag="ACHBJ1_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM9430653.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2164..3048
                     /gene="pstA"
                     /locus_tag="ACHBJ1_001718"
     CDS             2164..3048
                     /gene="pstA"
                     /locus_tag="ACHBJ1_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM9430654.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            3063..3869
                     /gene="pstB"
                     /locus_tag="ACHBJ1_001719"
     CDS             3063..3869
                     /gene="pstB"
                     /locus_tag="ACHBJ1_001719"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9430655.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3885..4643
                     /gene="pstB"
                     /locus_tag="ACHBJ1_001720"
     CDS             3885..4643
                     /gene="pstB"
                     /locus_tag="ACHBJ1_001720"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM9430656.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4656..5333
                     /gene="phoU"
                     /locus_tag="ACHBJ1_001721"
     CDS             4656..5333
                     /gene="phoU"
                     /locus_tag="ACHBJ1_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM9430657.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5910..6602
                     /locus_tag="ACHBJ1_001722"
     CDS             5910..6602
                     /locus_tag="ACHBJ1_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9430658.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            6599..7366
                     /locus_tag="ACHBJ1_001723"
     CDS             6599..7366
                     /locus_tag="ACHBJ1_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM9430659.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7466..9034
                     /gene="liaX"
                     /locus_tag="ACHBJ1_001724"
     CDS             7466..9034
                     /gene="liaX"
                     /locus_tag="ACHBJ1_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM9430660.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9331..9651
                     /locus_tag="ACHBJ1_001725"
     CDS             9331..9651
                     /locus_tag="ACHBJ1_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM9430661.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9651..10001
                     /locus_tag="ACHBJ1_001726"
     CDS             9651..10001
                     /locus_tag="ACHBJ1_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM9430662.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10168..11106
                     /gene="hprK"
                     /locus_tag="ACHBJ1_001727"
     CDS             10168..11106
                     /gene="hprK"
                     /locus_tag="ACHBJ1_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM9430663.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            11121..11951
                     /gene="lgt"
                     /locus_tag="ACHBJ1_001728"
     CDS             11121..11951
                     /gene="lgt"
                     /locus_tag="ACHBJ1_001728"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM9430664.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11995..13020
                     /locus_tag="ACHBJ1_001729"
     CDS             11995..13020
                     /locus_tag="ACHBJ1_001729"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM9430665.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            13035..13973
                     /gene="galU"
                     /locus_tag="ACHBJ1_001730"
     CDS             13035..13973
                     /gene="galU"
                     /locus_tag="ACHBJ1_001730"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9430666.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            14098..14538
                     /locus_tag="ACHBJ1_001731"
     CDS             14098..14538
                     /locus_tag="ACHBJ1_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM9430667.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14541..15143
                     /locus_tag="ACHBJ1_001732"
     CDS             14541..15143
                     /locus_tag="ACHBJ1_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9430668.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15202..16308)
                     /locus_tag="ACHBJ1_001733"
     CDS             complement(15202..16308)
                     /locus_tag="ACHBJ1_001733"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM9430669.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16518..17519
                     /gene="ccpA"
                     /locus_tag="ACHBJ1_001734"
     CDS             16518..17519
                     /gene="ccpA"
                     /locus_tag="ACHBJ1_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM9430670.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17856..>20181)
                     /locus_tag="ACHBJ1_001735"
     CDS             complement(17856..>20181)
                     /locus_tag="ACHBJ1_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM9430671.1"
                     /translation="KKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAALGIGIGA
                     GYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTISSDKISP
                     LLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQQILTDET
                     TFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAKGIFGVDA
                     KDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYREGQITKEQ
                     YEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADMSDSSVSD
                     AYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETGNVLMDNR
                     TGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSESRVSDYAT
                     TWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGSKQYAYEH
                     YLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDSITDNAGN
                     VIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKADWVGKTG
                     STNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAYQAKPDIF
                     GTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGPKKSAFKF
                     GVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         6417 a         3566 c         4292 g         5906 t
ORIGIN      
        1 ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta actttggggt
       61 atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa aagagacaca
      121 tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac aattatgaga
      181 atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa atgataaaat
      241 aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat tcttaccaat
      301 aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag gagaatccat
      361 tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg aaacttacca
      421 gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc
      481 tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc
      541 ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga tcactcctat
      601 tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa aactatttac
      661 aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag ttatattgaa
      721 ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg acggagaaac
      781 agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg ttgccgacac
      841 acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag atacgttgaa
      901 tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc
      961 atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat ttttagacta
     1021 tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata tccctgtttc
     1081 tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat ctttacacaa
     1141 aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg ttcaatttgt
     1201 acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag atgtgaaaaa
     1261 agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat tcatcagttt
     1321 tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg tagctagtaa
     1381 aggattggcc acctttttta ctgataagat caatgtattt gattttctgt ttggtacaga
     1441 ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat
     1501 cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat ttgccattgg
     1561 tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct tacagccggt
     1621 catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt tatcagttat
     1681 cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag ctggtacatt
     1741 tgtgttgttc gttatgatct tgccgactat cacaacgatg actgttgatg cattaaaggc
     1801 agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat ggcaaacaat
     1861 ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg tttttggtat
     1921 ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg cagcattgat
     1981 gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa tgggaatcgg
     2041 aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct
     2101 attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag gagagctgaa
     2161 ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat agtttcgggg
     2221 atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg aataccgcac
     2281 atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg tggaatcggg
     2341 atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag ttttcctatt
     2401 tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct aacagatgtg
     2461 attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg tttattcggt
     2521 ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg tgcattggct
     2581 ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt acaagcggtc
     2641 cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga aacagtgata
     2701 aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct aagttctgga
     2761 agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc acctgcgtta
     2821 gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt ccgtcaagct
     2881 gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc tgatggtgtt
     2941 tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt caacttcggc
     3001 gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg agaagaagca
     3061 aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa gagaaagaag
     3121 tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca atcaaaggag
     3181 tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc ggctgcggaa
     3241 aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca
     3301 ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga
     3361 tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag tcgatttatg
     3421 acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa
     3481 tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac agcttggaca
     3541 aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct cgtgcgatcg
     3601 cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac cctatttcga
     3661 caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc atcatcgtga
     3721 cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc tatttaggaa
     3781 aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt caggcaaccg
     3841 aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca aaagagatta
     3901 tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa gggatcgatc
     3961 taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc ggaaaatcca
     4021 cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg atcaccggaa
     4081 gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt gaactaagaa
     4141 aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc tatgaaaatg
     4201 tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat caagtagttg
     4261 aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg cataaaagtg
     4321 ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta
     4381 atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt tctactggga
     4441 aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc gtgacccata
     4501 atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat ggtcacttga
     4561 tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag acagaagatt
     4621 atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa tttgaagaag
     4681 aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc agcgccgtgc
     4741 acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa gttatcgaaa
     4801 acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt gaaatgatcg
     4861 cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg aaagctagct
     4921 cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa
     4981 aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc gattatgtga
     5041 aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta
     5101 cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat aatgcaacaa
     5161 tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac ttgaatgttg
     5221 ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg atcgtctact
     5281 tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc taaactgaag
     5341 ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg tgaaaaagct
     5401 gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat ttttcgtttg
     5461 gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga aagggtttgt
     5521 gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga atgcaaaaaa
     5581 aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt attcagcatc
     5641 aagccgcttt atatgaagta cctgtcatcg agatagaaaa agtacgaata aaagaggatc
     5701 atcaattgat tacaggaaaa ctgatgaaca cggatcaaaa aggagaatcg atttatttat
     5761 cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga caaatgtatc
     5821 tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat ggctggcttt
     5881 tcttcttcac aagtatcttc attggcgcca tgttgcttat tggtggaaaa cagggtctat
     5941 ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg ctcatcttta
     6001 ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt atagggatga
     6061 ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa atttcagctg
     6121 cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat cattggaaag
     6181 accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt tctgtttttc
     6241 tttctagcgt actgattgga acagctggag gaacactcga taccgttatc acagtcattg
     6301 cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag ttatggcttt
     6361 caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt ttgttcgtct
     6421 atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg tcgttcaaag
     6481 aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc agcttaggaa
     6541 tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg ctgaaacgat
     6601 gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta ttggcggtac
     6661 gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt tcctgttgtt
     6721 attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat ttttattgat
     6781 tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg cagcttttgt
     6841 gagtatactg atctttttat tttgtttcct actattgctt cctatcactg attttttagc
     6901 aatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata agactttagc
     6961 gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg gtgctgtttt
     7021 agatgcgagt gtagcaattt ctagtgggac attcgaagta tatcaggcaa atccgcaatt
     7081 atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat tggcgtcaac
     7141 agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc tctggtttat
     7201 tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc tagaatatac
     7261 gatggctatt cttaccacat tgtccgcact acttgttctt cctttaacct gcgtgactac
     7321 agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt tacaacttaa
     7381 gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa gcttgcaaac
     7441 aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag atttagtgaa
     7501 aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg caactgaaaa
     7561 agacgaaaag caaatcaaaa aagaagcaga caaggtaact gctagtcata aagaaaaaga
     7621 tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt ctgagccagt
     7681 tgatccaaag gaaagagaaa gacaagatca agaaaactta gaaaagatct tagatgaact
     7741 agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc aattcgctga
     7801 caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata caaaagaaga
     7861 attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag cagaaatcaa
     7921 agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag aagcagaatt
     7981 aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg ataagtttga
     8041 attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag gcgaaaaaat
     8101 gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa ctgtttcaga
     8161 aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg ggattgcgac
     8221 aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc ttgatatcaa
     8281 ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga aagttgaagc
     8341 taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct ctgaacggag
     8401 ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac gtgtgcgtgc
     8461 agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta aacttctaaa
     8521 tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat ctacgaatgg
     8581 taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg taaacggcaa
     8641 tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa atggtacagt
     8701 gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg atgtacgtct
     8761 tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg gaaatgtcaa
     8821 agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc ttggaagtat
     8881 caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga caaaccaaat
     8941 gttgcaattc cgtcgtgttt cagatggtga aatcgcgcaa gtgcaacttt caacgacaac
     9001 aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag atgttcagcc
     9061 ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa ttgtgcctta
     9121 ttgccgatag tctatctctt gaacaccgtt taccaagaga ttatgaacct gccaattatc
     9181 gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat atggtaattt
     9241 gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca acgattttct
     9301 tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac aaaatctaat
     9361 aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg gattgatccg
     9421 actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag tccaattatt
     9481 ttatacattt tattagcttt gattatccca tttgcgcgtt ctgcacgaac aaactatgga
     9541 cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga acaaacaaaa
     9601 aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta atgtcgtact
     9661 ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt tttcctgcga
     9721 tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg tctgtgttga
     9781 atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta gtcacattgg
     9841 gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt tttgtgggag
     9901 cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc ttgatgtcgc
     9961 ttgtcaatgt aattgtcagc gaacataaca gtagaaaata aaagaaagct gtgccgaact
    10021 atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt ggtaattaaa
    10081 taaaaacgtt ttttttaatt ggtagtactt ttggctagac aatgataaaa tgatagagaa
    10141 cactctaata gaaatgaggt agcatgaatg tcagaagttg taaaagttag ccaattagtg
    10201 aaaaaattat ctttggaaat cgtgacaggc gacgagaaaa gcttagaccg tgtcatcgta
    10261 actggggaca tctcacgccc aggactagag ttgactggtt attttaatta ttattcccat
    10321 aatcgtttac agctatttgg cagtaaagaa atcagttttg cggaacggat gatcccagaa
    10381 gagcgtttga tcatcatgag aagattatgt agtgaagata tgcccgcatt tattatttca
    10441 agaggattac cagctccaga agaaatgatc caggcagcaa ataaaaatgg gatagctgta
    10501 ctgcgttctc ccatctcaac ttctcgttta ttaggagaat tatccagtta tttagatggt
    10561 cgccttgccc ctcgaacaag tgtccacggt gttttagtgg atgtttacgg attaggtgta
    10621 ctgattcaag gtgacagtgg gataggaaaa agtgaaacag cgttggagtt gatcaaacgc
    10681 ggccatcgat tgatcgcaga cgatcgagta gatgtttatc agcaagatga attaacggtg
    10741 atcggagaac cgcctaaaat cctggaacat ctgattgaaa tccgaggaat cgggatcatt
    10801 gatgtaatga atctttttgg tgcgagtgcg gtacgtggct ctatgcaagt ccagttggct
    10861 gtttatctgg aagcatgggc aaaagataag aaatacgacc gtctgggctc tgaagacacg
    10921 accgttgaaa tcgcggaagt gggcattcca caaataaaaa tccctgtcaa aacaggacga
    10981 aacgttgcaa tcatcattga agtagccgcg atgaatttcc gtgcacgtac aatgggcttt
    11041 gacgcaacga aaacatttga agaaagatta agtcgcttga ttgaagaaaa ctcaggcaac
    11101 gactagcaag gagtgacaat atgttagcac aaatcaatcg agttgctttt gagcttgtag
    11161 ggattcctat ctactggtat gcattgatca tcgtatcagg tatcattatc gctatgtggc
    11221 ttagcagcag agaggctgtt cgggtaggat taaaagcaga tgatgtgaca gattttatgt
    11281 tgtggggatt gcctatctcc attattggcg ctcgcttata ttatgtttta tttgatcttc
    11341 cccagtatat agctgatcca atccaaatat tcaatatccg ttcaggaggc ttagctattt
    11401 atggcggact gattgcaggc gcgatcacgc tgtatttttt tactaaatat cattttattt
    11461 ctttgtggac ttttttagat atcgctgcac caagtgtatt attagcacag gcaatcggtc
    11521 gttggggaaa tttcatgaac cacgaagcat atggaccaga cacgacgcga agcttcttag
    11581 aaagtctgca tttaccgcaa ttcatcattg ataatatgta tatcgaaggg atctatcgtc
    11641 aaccaacatt tctttatgaa tcactttgga gtttgattgg atttattttg cttttgtttt
    11701 tacgaaagaa accacacctt ttgaaaaaag gcgaagtcct tttatgttat ctaatgtggt
    11761 atagtttcgg gcgtttcttt attgaaggat tacgtacaga cagtctttac tttttaggtg
    11821 tgatccgtat ttctcaatta ttatctgctg tgttatttgt cggtacattg attctattta
    11881 tttggcgcag aaaaaaagga aatttaccgg attatgaccg ctcattagga aaaaatcaac
    11941 aaattatctg atttttagag cgttaaaaat gaaaaaaatt tcaggagtgg taaaatgaat
    12001 tatagagtag cggttttagg accaggctct tggggtactg ctttggcaca agtcctcgcc
    12061 gaaaacggac atgacgttcg gatttgggga catcgtgctt cacaagtaga agaaatcaac
    12121 acccaacaca cgaatcaacg ttatttacca gatcattatc tacccgattc catccaagca
    12181 ttcaccaata tggaagatgc agtaaaagac gcagatgcgg tgctattcgt cattccaacg
    12241 aaagcaatac gttctgttgc aaaagaacta gtgcctaaat tgaatacaaa gccagtgatc
    12301 atccatgcaa gtaaaggatt agaacaggga acacataaaa ggatctctga catcttgtta
    12361 gaggaaatcc cagcagaaaa aagaaaagct gttgttgttc tttctggacc aagccatgca
    12421 gaagaagtag caaaacatga catcactacg atcacagctg caagtacaga tgcaaaagca
    12481 gcaaaatatg tacaagagct gttcatgaat aattattttc ggatctatac caatcctgat
    12541 gtgatcggtg tagagatggg ggcagctttg aaaaatatta ttgctattgg tgctggcgct
    12601 attcacggct tagggtttgg cgataatgca aaggcagcaa tcatgacgcg cggattagct
    12661 gagatcagcc gtttaggtgt agcaatgggt gccaacccgc tgacctttat cggattaagc
    12721 ggaataggcg atctagtcgt tacttgcaca agtgttcatt ctaggaactg gcgcgcaggc
    12781 aacttgttag gacaagggca gcccctagaa gaggtgctag ccaatatggg aatggtcgtt
    12841 gaaggtgtga atactaccca agcagcgatg gaactagccc aaatgatgga aatcgaaatg
    12901 cccatcactc aaacaatcta tgaagtgctg tatgaacaaa aagaaatcaa aacagccgca
    12961 aaagaaatca tgttacgtga tggaaaaaaa gaaaatgaat tctcattaac aattttttga
    13021 aggagcgaat gtatatgaaa gttaaaaaag cagttattcc agcagcagga ttaggaacaa
    13081 gatttttacc agcaacaaaa gctatggcaa aggaaatgtt gccaattgtt gataaaccaa
    13141 caatccagtt catcgttgaa gaagcgttga aatcaggtat cgaagacatc ttgatcgtta
    13201 caggaaaagc taaacgacca atcgaagatc actttgattc aaatattgaa ttagaaacaa
    13261 acctaagtga aaaaggaaag accgaattat tgaaattagt agaagaaacg acagatgtca
    13321 acttgcattt catccgtcaa tctcatccaa agggtttagg acatgccgtt cttcaagcaa
    13381 gagcctttat tggaaatgaa ccatttgtcg ttatgctagg cgatgatata atggaagacg
    13441 aagtaccatt gacacagcaa ttaatcgatg attacgaacg tactcatgct tctacgatcg
    13501 ccgttatgca agttcctcat gatgaaactt caaaatacgg aattatcaat ccaggagaag
    13561 tattggaaga tgggttatac aatgtcaaaa actttgttga aaaaccagat ccaagcaaag
    13621 caccaagtga tttagctatt attggacgtt acttattgac tccagaaatt tttgatgtat
    13681 tgtccgagca acaaccagga gccggaaatg aaatccaact aactgacgca atcgatacat
    13741 tgaataaaac acaacgcgta tttgcccgta aattcacagg taaacgttac gatgtcggtg
    13801 acaaatttgg ttttatgaaa acaagtattg aatatggtct aacacatcca gaagtaggtg
    13861 cacctttacg tgagtacatc ctgtctttag gtaaaacatt agaagcagaa ggaagcaaga
    13921 tcgatcataa tccaaaagga ataacagaaa tcaaagaaga aaagaaaaaa taaccctttt
    13981 ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt tttatttgtt
    14041 taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag tttgatcatg
    14101 tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact tgtcgttttt
    14161 atcgttcgtg tgttgttgca agtttctaaa actttagaaa aagtagatca gactgtagca
    14221 gaagcaaata cgacgatcga aatcgtcaca aaagacgtag atcttttgtc tagacaagta
    14281 gaaggcttac tggtcaaaag taatgaactg ctcttggata tcaatggaaa agtcgcaacg
    14341 atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcaga acttaatcat
    14401 tctagtaaaa acatcgcgac gaaagtcggc ggaatcggta aaacgacagc taaagcgacg
    14461 gttgccggaa aagtcggtgg gacagcgatg aaatttttta aaaacaaaaa acatactgaa
    14521 acgacaggag gaaaataaaa atggctaaaa aaggtggatt tttattaggt gcagtagtcg
    14581 gaggcacagc cgcagcaatc gccgcgttat tgttagcacc aaaatctggt aaagaacttc
    14641 gggatgaatt agctgcccaa gcggatgatt tgatggatat ggcttcagat tacacagata
    14701 tggcgaaaga aaaatcgact gagttaacag gtattgcaaa agaaaaagcg caagatcttt
    14761 ctcaacaagc aggtgatcta gcaggtagtg tcaaacaaaa agtaagtgaa tcagcagatg
    14821 aagcaggaag tgaatctgac gatatcttgt taaatcttaa agaacaatca gctgacttat
    14881 ctaatcgttt caaaaaaacg gcagaagata tgaaaaaacc tgcagatgaa ttaggaagta
    14941 ttgctaaaga tacttcagaa gatatcatga ttgatgtaaa agacgctgct ggtaaagcag
    15001 gagatactgt caatgaagga atgtcagaag caaaaccagt tgcagatgac tcaaaagaaa
    15061 tgatgaaaat ggcaaaagaa gatgcgaaag aagctaaaaa agaaataaaa gatacaatga
    15121 cagattcatc tacaaatcac taaaatcaca aatttataga ataaaaaggt gggagcggac
    15181 tagctctcac cttttttact cttatttttc taaaacaagt aattcttttg gtgtagtcgt
    15241 gaatggcaca cagccatcgc ttgtcacata tacacagtct tcgatacgta cccctacttt
    15301 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcga ttactaagtc
    15361 atttccttct acaagggaag ggaattcgtg gacggttgtt ccgatcccgt gacctaaacg
    15421 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
    15481 ttcacctgct gtcacacctg gacggacggc ttcttgtgcg gcaagctgtg cttcaagagt
    15541 gatgttgtag attttttctt ggaagtcggt tggtttttga taagcaattg tacgagtagc
    15601 atcactgcaa tagccattcc aaacaacgcc aagatcgaat aacacaagtt cattcggtga
    15661 aatcttcgta tttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    15721 tgtgtcgaaa gacatttgtg aaacaccttg ttttttcaat tggtattcaa tttctgcaat
    15781 gatcgcttgt tcagtcacgc cttctttcac tgctttaaag ccaatctcaa atgcaacatc
    15841 ggcccattta ccagcttcga tcagtttgtc tttttcctct agagttttga tcagttgtag
    15901 acgttggatg atggaagtta gatcagtgta agaatccgtt cccgtcaaga tatcagagat
    15961 agcatcgaag cgctcgacag tcaaggcgtt cttttcaatc cctacattgc caggctgacc
    16021 atagcgagca cgaagttctt ttccgatgat ctcccaaggg ttttcgctgt ctaaatagcc
    16081 acggacatca tacgcccaac tactgttttg agcatcttca acttccaaag caggagtgaa
    16141 aaggaaagga tcttgttcaa gagggataaa aagagctagt acacgctcat gaggatcact
    16201 ttcgtatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attcgacttg
    16261 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    16321 tttctaaatt aattttgtat tcaataattg taccactgat ttattaaaag accaacgttc
    16381 taaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattcttg
    16441 agattatttg atattctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    16501 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    16561 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gttaaaccag
    16621 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aacgcagttg
    16681 cgcgtggctt ggcaagtaag aaaacaacaa cagttggtgt aatcatccct gatgtcagca
    16741 atatgttctt tgcttcttta gcacgtggta tcgatgatgt ggcaacaatg tacaaataca
    16801 atattatctt agcaaattca gatggcaacg accaaaaaga agtcaatgta ttgaataact
    16861 tactggctaa gcaagtggac ggcgtgatct tcatgggtca ccatatcaca gacgaaatcc
    16921 gtggagaatt ttcacgttca aaaactcctg ttgtattagc tggctctatt gatccagatg
    16981 aacaagtagg tagcgtaaac attgattatg tatccgcaac aaaagatgca gtaaacaaat
    17041 tggcaaaaag cggcaataaa aaaatcgctt ttgtcagtgg tgcgttgatc gatcccatca
    17101 atggacaaaa tcgtctgaaa ggctataaag aagcattaag cgaaaataac ctaccatata
    17161 gcgaaggatt gatctttgaa gcacaataca acttcaaaga tggtctatct ctagtcgatc
    17221 ggatccataa tagcggtgca acagctgtct atgtttcaga tgatgaatta gccatcggta
    17281 ttttggatgg tttattagac cgtggcgtga aagtaccgga agaattcgaa atcatcacaa
    17341 gtaacaattc actactgaca gaagtagctc gtcctcgttt aacaagtatc acacaaccat
    17401 tatacgattt aggggcggta tctatgcgcc tattgacgaa aatgatgaat aaagaagaag
    17461 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatct acaaaataat
    17521 aaaaggacaa ccattcagag gttgtgtgcc tgccgtttaa ctctgcgtta caaggagcaa
    17581 tttcaaaaaa cagcatctca tgtttgatga atattgtgac tcgtgattcg gagttggcag
    17641 gagaacaccg tttatttggg aaaaggggtt atgacaaaat tttgttataa tccttttttt
    17701 gtgtcttaaa aaagagagaa agtggttgaa attacattta tcgtatataa aacaatccgt
    17761 gtatagatgc tgcattttgt tcaccaccat caaaaaaata agggactgtg acaatactat
    17821 tgtcacaatc ccttattact gtgtattcta ttaaatcaat catctttctc atctgattct
    17881 ttagttgctt gattcgctct tgcataagct gaagcggtgt tccagtaatc tgtatagttt
    17941 tcatcggtac cgccaacgcc aaatttaaag gcgcttttct tcggtccatt tttcgcccat
    18001 agcgattcaa ctgtttttcc tggtgtttgg atggattttt tattgacagt aacttttcca
    18061 ggtgtttgac cagtaaatcc agatactttt ttcttcatca catcagatga taattcgaat
    18121 ttctcatcag tgccaaaaat gtcaggtttt gcttggtaag cttgattgat cagattggca
    18181 agataattag ccgaacgtat ccttgctttg ctgtccattc cagtattgtc atcgtgccct
    18241 gcccagctac tgatggtaat agacggagta gagacaatta gccatgaatc tcggtattca
    18301 ttggtagaac cagtttttcc gacccaatct gcttttccta gatttccgtt taagcttgag
    18361 atggcatctt tgaatggtgt agtgattttt gcattgatca cactacgcat catgtcattc
    18421 atgatcgatg ccgttgcttc cgaatagatc cggacagagt tgctttcgtg tttataaatt
    18481 acatttcctg cgttatctgt gatggaatcg atgatatagc cttcttcata gacgccgccg
    18541 tttgctaaag cctgaaatcc gttggtctgt tgaagcgtcg tgacatctac tgtacctaaa
    18601 ggagcagatt caacgcccca attgtcattt gccgggtagt tcatctttgc caagtagtgc
    18661 tcgtaagcat attgtttcga tccaccggaa ttcaacacat cttgatataa gtgataagcc
    18721 gggatattgt tggaccattc tagtgactca cggacggttt ggaaggtgtt cgatccttcg
    18781 ttagtagcat tgacgatttt ctcgccagca ttttctcctt cttgccaagt tgttgcataa
    18841 tctgatacac gggattctga tccgataagc cccatgtcga ttgcaggtcc ataaacaaga
    18901 actggtttga ttgatgagcc agcttgacgt tctgtatcga aggcatggtt attttgattc
    18961 tgactgtaat tccgtccgcc aacgaatccg tagatgcggc cagttctgtt atccatcaat
    19021 acatttccgg tttctacttg actgccagca ccatcatcta acatgtatcc ataattttca
    19081 acgccatttt gcaatgcagc atagacatct ttatcgattg tcgaatggat tgtatatcct
    19141 ttattttgga tcgtttgctg tgccttttga taataagcgt cactgaccga tgaatcagac
    19201 atatcagcgt tgtctttttc agccaattgt tgtgcaatga ttcttgttgc ttcattcatc
    19261 acagtataat acaagaattc tctgtcagtc tgttctgcga tttgttgtgg tagaaagtct
    19321 tttgtaaggt cataggcttt tgcttcttcg tattgttctt tcgtaatttg gccttcacga
    19381 tacatactga ataaaacaaa atcttttctg gcaaggccgg cagaaagatc ttcttttagc
    19441 gcgcctgtgt tcgtgtatgg actatacgtg atcgggcttt ggggtaatcc agcgatataa
    19501 gcagcttgcg gcagcgtcac atcttttgca tctacaccaa agattccttt tgcagcttcc
    19561 tggactccag caatgttttg tcccttatta ttacgaccaa atggtgagac attcaaatac
    19621 gtagcaatga tctcatcttt tgaaaagtat ttttctactt gtgcagaaag aatgatctcg
    19681 tttgctttac gtttgaatgt tgtttcatct gtaaggatct gctgtttgac taattgctgt
    19741 gtcaaagttg atccaccaga agagccaata ccagtagctt cagatactaa tgctcgtaaa
    19801 acagctttag gaacgtaacc ttggtgttta tcgaagtatt catcctctgt gctgataatt
    19861 gcagttttca ataaaggtga aatcttatca gatgaaatag tcgtgcgcat cagatctgtc
    19921 tggatttttg atatctctgt attatctgca taaacaatct ttgacgtttc tgtaatgttt
    19981 cctagttctg tctgcagtgc ttttttagtc ggcaattgtg tgtcttcgac gagataggca
    20041 aaatagccag cgcctattcc aatacctaga gctgcgaaca tcaaaagaag aacagtaata
    20101 aaaacagtca atgattgaaa aactctaaaa ataatcttag gaacaaacca tttttctttt
    20161 tttcttctat tctgtttttt t
//
LOCUS       DAYYNQ010000053        20032 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000053 DAYYNQ010000000
VERSION     DAYYNQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.053"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1500
                     /locus_tag="ACHBJ1_001736"
     CDS             <1..1500
                     /locus_tag="ACHBJ1_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFM9430672.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDD
                     TLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHBJ1_001737"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="ACHBJ1_001737"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM9430673.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3076..3351
                     /locus_tag="ACHBJ1_001738"
     CDS             3076..3351
                     /locus_tag="ACHBJ1_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM9430674.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3357..4124
                     /locus_tag="ACHBJ1_001739"
     CDS             3357..4124
                     /locus_tag="ACHBJ1_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM9430675.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4292..6121
                     /gene="typA"
                     /locus_tag="ACHBJ1_001740"
     CDS             4292..6121
                     /gene="typA"
                     /locus_tag="ACHBJ1_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM9430676.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6382..8154
                     /gene="recQ"
                     /locus_tag="ACHBJ1_001741"
     CDS             6382..8154
                     /gene="recQ"
                     /locus_tag="ACHBJ1_001741"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM9430677.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8268..8570
                     /locus_tag="ACHBJ1_001742"
     CDS             8268..8570
                     /locus_tag="ACHBJ1_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM9430678.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8573..9736
                     /locus_tag="ACHBJ1_001743"
     CDS             8573..9736
                     /locus_tag="ACHBJ1_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9430679.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9762..13190
                     /locus_tag="ACHBJ1_001744"
     CDS             9762..13190
                     /locus_tag="ACHBJ1_001744"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM9430680.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13211..14335
                     /locus_tag="ACHBJ1_001745"
     CDS             13211..14335
                     /locus_tag="ACHBJ1_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM9430681.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14316..14762
                     /locus_tag="ACHBJ1_001746"
     CDS             14316..14762
                     /locus_tag="ACHBJ1_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM9430682.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14746..15084
                     /locus_tag="ACHBJ1_001747"
     CDS             14746..15084
                     /locus_tag="ACHBJ1_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM9430683.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15203..15763
                     /gene="rsmD"
                     /locus_tag="ACHBJ1_001748"
     CDS             15203..15763
                     /gene="rsmD"
                     /locus_tag="ACHBJ1_001748"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM9430684.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15760..16251
                     /gene="coaD"
                     /locus_tag="ACHBJ1_001749"
     CDS             15760..16251
                     /gene="coaD"
                     /locus_tag="ACHBJ1_001749"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM9430685.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16241..17284
                     /locus_tag="ACHBJ1_001750"
     CDS             16241..17284
                     /locus_tag="ACHBJ1_001750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM9430686.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17443..18171
                     /locus_tag="ACHBJ1_001751"
     CDS             17443..18171
                     /locus_tag="ACHBJ1_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM9430687.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18287..18532
                     /locus_tag="ACHBJ1_001752"
     CDS             18287..18532
                     /locus_tag="ACHBJ1_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9430688.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18529..18747
                     /locus_tag="ACHBJ1_001753"
     CDS             18529..18747
                     /locus_tag="ACHBJ1_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM9430689.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18883..19422
                     /locus_tag="ACHBJ1_001754"
     CDS             18883..19422
                     /locus_tag="ACHBJ1_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM9430690.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19415..>20032
                     /locus_tag="ACHBJ1_001755"
     CDS             19415..>20032
                     /locus_tag="ACHBJ1_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9430691.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQK"
BASE COUNT         6797 a         3465 c         4395 g         5375 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
     3001 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
     3061 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
     3121 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
     3181 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
     3241 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
     3301 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
     3361 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
     3421 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
     3481 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
     3541 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
     3601 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
     3661 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
     3721 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
     3781 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
     3841 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
     3901 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
     3961 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
     4021 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
     4081 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
     4141 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
     4201 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
     4261 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
     4321 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
     4381 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
     4441 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
     4501 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
     4561 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
     4621 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
     4681 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
     4741 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
     4801 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
     4861 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
     4921 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
     4981 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
     5041 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
     5101 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
     5161 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
     5221 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
     5281 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
     5341 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
     5401 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
     5461 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
     5521 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
     5581 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
     5641 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
     5701 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
     5761 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
     5821 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
     5881 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
     5941 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
     6001 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
     6061 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
     6121 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
     6181 tgtccaactc ttttctgtgg agaagtcatc tttgtcaatg gacataaatt cagaaaatac
     6241 gccgaaagac gtttaaaaga cttttaaaaa acttatttct agagagtttt tgtttcataa
     6301 taagataatg tttcacgaaa ggagatcaac ttgtccttcg aagaaaatcg aagtaaaatg
     6361 gcaagagaaa ggagcgggaa gatgaacgac ttattaaaac aatactttgg ctatgatgag
     6421 tttagacctg gtcaaaaaga aattatccag aaagtcattg atcaggaaaa tgtattagga
     6481 atcatgccta ccggaagtgg gaaatcaatc tgttaccagc ttccagcttt actattagat
     6541 gggcttactg tggttgtgtc ccctttaatc tctttgatga aggatcaggt ggacgcggcg
     6601 aatcaattag gtatacccgc cacttttatc aatagctcgt tagatggtta cgaaacagct
     6661 agacgcttcc aagaaatcga tagacagcag taccgtttac tttatatcgc tcctgaacgt
     6721 tttatcatgc cggattttat ccaagcaatg aagcgatgga acgtccgtat gatcgctatt
     6781 gacgaggcac attgtatctc tcagtggggg catgatttcc gcccaagtta tttacagatg
     6841 gcgaaccagc tagatcaact gccaaatcgt cctgtaattg ttgctttgac agctacagcg
     6901 actgtgcaag tagctgctga cataaaaaga cttttaaaaa ttccagaaaa caaccatatt
     6961 cagactggtt ttgaacgaga aaatctacga tttcaagtaa ttaaagatca aaaaaaagaa
     7021 caatatctaa tcgaatattt aaagatcaac aaaaatcaat ctggtatcat ttatgcagca
     7081 acgagaaaag aagtagaccg tctctatcat ttgcttaaaa aattcgattt ttccgtgggt
     7141 agatatcatg gtggtctaaa tgagaatgag cgaacagaaa tgcaagaagc ctttttatac
     7201 gatcgcttgc agctgattgt agcgacaaat gcatttggta tgggtataaa taaaagcaat
     7261 gtccgttttg ttatccatta tcaaattcct ggctctctag aagcatacta tcaggaagct
     7321 ggtcgggcgg gaagagatgg tctttccagt gaagccatcc tgcttttctc tcctcaagat
     7381 atccaagtcc aaaagttttt tatccagcag tcccaaagag aagaagggca aaagcaaaaa
     7441 gaatatgaaa aattaaaagc gatgacagaa tacgtgtata ttgagtcctg tcttcagcaa
     7501 tacatcctaa actattttgg cgagacaagc agtccgtgca accgttgtgg taactgttta
     7561 gatgacagag aaatagtaga agtaacgaca gaagcacaga tggttctttc ttgcttaaaa
     7621 cgtatgggtg agaactatgg taaacaaatg ctgatgaaag tcttagcagg atcgaaagaa
     7681 caaaagctgc aagctttagg ttttggacat ttatctacct atggactgat gaaaaaccaa
     7741 tcgcaaaaag aaacgatgca gttaattgaa tatttgatat cgaatggcta tcttctgacc
     7801 atcaatggtg agtatcctgt tttgaaagta acagaaagag ggatacaagt attgaaagga
     7861 caagaatctg tttatcgaaa ggaacccaag aaagtccagc agctatcgga tgaagaaacc
     7921 gatacactat ttgaagtgct gcgggaactg cgaacggatc tagcatcaga agcaggcgtt
     7981 cccccgtatg tcgtattttc agattccaca ttgaaagaaa tgagccgaat acgtccatca
     8041 agcagattgg agatgcttca gataaaagga gttggccaaa gcaagcttga taaatatggc
     8101 gaagcctttt tatctcggat aaaaaatgcg gactctaacg ttctggataa gtagaaagaa
     8161 cgaatacgta aaaatgaata aaaacaactg gttaaatctg ttttttttgc tccttatgaa
     8221 aatcaaatat gttataatta aggaattaga atgggaggta agcacagatg gattctttgt
     8281 cgaaaaaact tgcgatccaa gtattaaatg atgaagcaga acggatcaaa cgattaatcc
     8341 gaaatcaaaa caacagtctc tgtatctcac aatgcaaagc cttcgaagaa gtggtagata
     8401 cacagatgta cggcttttcc cggcaagtcg gttttgctgt ccgtatagga gtaatcggaa
     8461 agaacgaggg ccagctgatg ttaagtgagc tagaacgaga attgaatcag ttatatagtg
     8521 aggtttacca agaaatttat gataaaaaag aagttggcaa ggaggaatag acttgcctaa
     8581 gaaagtgaaa aaaaggcatc tattagacta tagcatcttg attccctatt taattttatg
     8641 tgtattggga ttgatcatgg tatatagttc tacgtcgtat ctattattag aaaacggaca
     8701 aaatccgtcc gcatcagtca ttaaccagag tattttttgg gtgcttagct tgattgctat
     8761 cgcactactt tataaaatga aaacagatgt tttgaaaaac cagcgtttga tcatggcagc
     8821 catcgcagtt ttgacgattt tgctgctgat tgttgtgttt tttggtaagg agatcaatgg
     8881 ggcgaaaggt tggctgcaaa ttgcaggatt ctcgatccaa cctgccgaat acctaaaaat
     8941 catttctatc tggtacctgt ctctgacttt atctaaaaga caaaacagtg tacaaaaaga
     9001 ttttcttggg actgtgaaac gtccacttgc aatggttatc ggacttactg cattagtcgc
     9061 tattcttccc gattttggta atgcggcagt tatctttttg attattttgg ttctgctttt
     9121 agccagcgga gtcaattatg tgtatacttt gattgtagga gtaggaggct tttgtctaag
     9181 tacttttacg atctggctga tcaatatcac aaacggaaag atcttacccg gacgtttaca
     9241 gtatatctat aatcgatttg ctatttacca aaatcctttc tcagacgaat taaataaagg
     9301 gcatcaacta gtcaacggct actatgccat gttcaacgga ggactatttg gcaggggatt
     9361 aggtaacagt atccagaaaa aaggattttt acaagaagca caaaccgatt ttatttatgc
     9421 aatcgtagtg gaagaactgg gagtgattat ggggattttg atattagcgt tgctattctt
     9481 tatgattgtg cgcattgtcc ttgtaggtat ccgttcaaaa gatccgttca attcgctttt
     9541 gtgcattggg atcggcgcaa tgtttttgat ccaagtattt gtcaatcttg gaggaattac
     9601 tggcgttatt cctttgacag ggatcacgtt cccattttta agtcaaggag gctccagttt
     9661 gctgatgctt tcgatttgtg tcggctttgt cctcaacatc agtgcggatg aaaaacgcaa
     9721 gagcttaggt ttataataaa aaaagatgta ggagtggttc gatgaaaaaa gttttagttg
     9781 ctaacagggg tgaaatcgct gtacgtgtat ttcgtgcttg tacagaatta ggaattaaaa
     9841 ctgttggaat ctatgcagaa gaagatgaat attccgtaca ccgtttcaaa gcggatgaag
     9901 catatttagt aggacaagga aaaaaaccga ttgatgcgta tctagatatt gaagggatca
     9961 tttctatcgc gaaagaatgc ggagctgacg ctattcatcc gggttacggt cttttgtcgg
    10021 aaaacttgaa ttttgcgcaa cggtgcgaag aagaaggcat catttttgtt ggacccaaac
    10081 tgcatcattt agatattttt ggagataaaa tcaaagcaaa agctgcagct atcgaagcgg
    10141 ggattgcttc gattccaggc acagatggac caatcgcttc tatagacgat gccttggagt
    10201 ttgcgaaaca atacggttat ccgattatga tcaaggcagc tctaggcggc ggcggacgcg
    10261 gcatgcgtgt agctcacgat gaaaagagtg caagagaggg ctacgaacgt gcgaaaagcg
    10321 aagcaaaagc agcctttggt agtgatgaag tgtatgttga aaaatacatc gctaatccta
    10381 agcatatcga agtacagatt ctcggggata cgcatggaaa cgtcatccat ctttttgaaa
    10441 gagattgttc tgttcagcgc cgtcaccaaa aagtagtaga agtagcgcct tgtgtctcta
    10501 tgaatgaaca gcaacgtcaa aaaatctgcc aggcagctgt ccaattgatg aaatatgtcg
    10561 ggtatgtcaa cgcaggtaca gtagaatttc ttgttgaagg agatgatttt tactttatcg
    10621 aagtcaaccc tcgcgtccaa gtggaacata cgatcacaga aatgattaca gatattgata
    10681 tcgtaacaac gcaactgttg attgctcaag gtttggatct gcataaagaa atcggattgc
    10741 ctcagcaaga aggaattaaa ttaaatggtt cagccattca atgccgaatc actacagaag
    10801 acccgttgaa taacttttta ccagacacag ggaaaatcga tacgtatcgt tcacctggtg
    10861 gttttggcgt acgtctggat gtcggaaatg cttacgcggg ctatgtagtg acaccgtatt
    10921 ttgattcatt gctagtaaaa gtatgtacgc atggtgctac ctttgaaaca gccattcaga
    10981 aaatggaacg ttgcttgaaa gaatttagaa tccgtggagt gaaaacaaac attccattta
    11041 tgctcaatgt catcacacat cctgaattcc aatcaggaaa tgcaaaaaca accttcattg
    11101 atagtacagc aacattattt gaatttcctc gactacgaga ccgcggaaac aaaacgatga
    11161 aatatatcgg tgaaattact gtgaatggtt ttccaggtat cgaaagtggc gaaaaaccat
    11221 tttatgaaga accacgtatg cctaaagatc taatcacaag aactgattac gtaacggcta
    11281 agaatgtatt agatgcgaag ggtgccgatg ctttggtcga atggatcaaa ggacaggaaa
    11341 atttgttgtt gacggatacg acatttagag acgcccatca aagtctgcta gcaactcgtg
    11401 ttcggacaaa ggatttcaag cagattgcac gtttgactgg agaaggtctt cctgaactct
    11461 tttcaagtga gatgtggggg ggagcaacat ttgatgtggc ttaccgcttt ttgaatgaag
    11521 atccttggca acggttgaga aaaattcgtt ctttaatgcc taatacgttg ctgcaaatgc
    11581 ttttcagagg ttcaaatgcc gttgggtatt ctaattatcc tgacaatgta ttggtggaat
    11641 ttgtcaaaga agcagcagcg caaggaatcg atgtatttcg aatctttgat agtttgaact
    11701 ggactccaca aatggaaaaa agtatccaag ctgtacggga tactggtaaa atcgcagagg
    11761 cagctatttg ctatactggc gatatcaacg atccaagtcg ggcaaaatac aatgttcagt
    11821 attataaaga catggcaaag gaactagaac aactgggtgc gcatatcatt gcaatcaaag
    11881 atatggctgg gctgcttaaa ccgcaggcag cttatcgttt gattagtgaa ttgaaagaaa
    11941 cgacagatct gccaatccat ctgcatactc atgataccag cgggaatgga atcatcactt
    12001 attctgctgc ttcaaaagca ggagtagaca tcgttgacgt ggcaatgagt gcaatgagtg
    12061 ggaatactag tcagcctagc atgagcagct tgtattatgc actggtcaat ggtccacggc
    12121 tgccagaaat cacgattgaa aatgcacaga aattaaacca ttattgggaa gatgtgcgca
    12181 tgtattacaa accgtttgaa aacggcttga atgctcctga aacagaagta tatatgcatg
    12241 aaatgccagg aggacagtat tcgaatctac agcaacaggc aaaagctgtc ggattaggtc
    12301 atcgctggga tgaaatcaaa caaatgtatc atactgtcaa cttgatgttt ggcgatatcg
    12361 ttaaagtaac tccatcatct aaagtagttg gagatatggc actatttatg gtccaaaatg
    12421 atcttacaga agaagatatt tatgaaaaag gcgaaacact aagtttccct gaatctgttg
    12481 tgactttctt ccaaggagaa ctagggcaac ccgttggcgg atttcctaaa aaattgcaaa
    12541 agattattct taaaggcaga cctgcattga atgaacgtcc aggtcttttg gctgaatcag
    12601 tcgacttcaa cgaagtaaaa aaagaattgg cagaaaaaat tggctatgaa ccaaaacaag
    12661 acgaagtatt aagctatttg atgtatccgc aagtcttttt ggactatcaa aaagcatata
    12721 atcaatttgg cgatgttaca ttactggaca caccaacatt tttccaaggt atacgtttgg
    12781 gtgaaacaat caatgtacaa atcgaacgcg gaaaaacgct tatcattcgt ttagatgaaa
    12841 tcggagaacc cgatatcgaa gggaatcgcg tactgttctt caatttgaat ggacaaagac
    12901 gtgaaatcac tatcaaagat aattcgatca tcagtacggt ccaaacccga ttgaaagctg
    12961 aaccgacaaa ccgagaacaa attggagcaa cgatgtctgg ttctgttttg gatgtattag
    13021 tgaaaaaagg cgataacgtt aaaaaaggtg atacgttgat gattactgaa gcgatgaaaa
    13081 tggaaacggc catcgaagca cgttttgacg gagaagtcgc acatgtatat gtgtcttcag
    13141 gagacactat ttcgtctggt gaccttttga ttgaagtgac tgagaaataa taagcgaaag
    13201 gtggggaaac atgaaaagac tgggtttgtt ttggctgatt ctgctattag ttattgtggc
    13261 attctacatg gaaccagtct tgtttccgcc aaaacttgaa gtgaaggaaa actctgatca
    13321 acaaatatca aaccaaatga caacgtggaa ataccaggag cttaaagcaa ctggtttttc
    13381 tacttatatc ggaaaaccga tcgatcagct ggaaaaggat tttggccaag cctatgatcg
    13441 attaactagc ggatttggtt ttgaaacaag atattatgaa aataaagcta gtcatctcac
    13501 atttgaagcg aacatccaag acagcagagt cacaactgtt aaagtactgc aaacagggcc
    13561 tgctgatatc gcgccgtttt atttagggat gacgatgcaa gatcttacga aaatcactac
    13621 tatctatact aatttcactt ttgagtacca tgatacagaa gttggaatcg aactaatgga
    13681 agaggatatg aattatcgtc cgctaattgc ttttgataac gacacttttg ctattttatt
    13741 ttttgatcaa acaacaaacg gcctattttc agttgtgtat ttagataaag atagtctttt
    13801 gaaactattg ccatatcaag tgtttggtga aggccttcca cattatcaag cagataaatc
    13861 ggctgattgg gaatcaatca atcgcgttaa agaacgaaaa agtacgactt tattaaatat
    13921 gcttagaaga gaagacgaac ttcccatata caatcaaaca cttgcctttc aaaaccacag
    13981 tcagctgcta ctacatgatt ttttgacaag gcctgaggaa atattgtcag aggaacgtat
    14041 ggctgattgg caaaaatcgc taggcagtgc tcaaacgaca gtgaaattct cactgacgaa
    14101 tgatgaattg gagaagttaa tcagtaaaca aaaattagaa aatactagtg ggctatttat
    14161 tcatccggcg attgacgcta ctttttcgtt tttatattgg tacagcgatc cttacaacca
    14221 tgaccgtttc atggacccgg gaacggattc tgtaggcatt gctttttcta aagaaaatat
    14281 gttagtctta ttgcaagaag aaaacggaga aagtagtgat agcagtgatc gttaacgaag
    14341 agctgtttca actggaggat caatgcctcc aagtcgctga aatgataaaa aagagtaaaa
    14401 cgatggcttc ttatcttgaa cataaacagt caatggaaga atctgcaaaa gtttgccagt
    14461 tgaaagaagc gttcttgtct aagaaagaag catttgaaaa tgtagcggca tatggtgagt
    14521 atgcacctga ttatcgtgaa aaaaaacgag ctgttcgaaa agcaaaaaga cagcttgata
    14581 tggatgagca tgttgcagct ttccgatata gtgaaacaca gctgcaaagt ctgttggatc
    14641 aaattgtcca agtacttgcc gaagaagttt ctaatgaaat caaagtagat gcaggtagcc
    14701 cgttttttga aacgaaaaaa cactttggat gcggaggtaa ttgtcatgca attagacaat
    14761 agtgaatttg aaataacaaa acgacgcggg ctgatcgtat gggtctacag cttaaaacaa
    14821 ttaaagaatc tgaaaaagtt cggcttgttg cattatgtct caagaagaat gaaatatgtc
    14881 gtgctttact taaatgaaga gacattggaa gaaacagaag aacgaatccg taaattacat
    14941 tttgttcgaa acgtagaacg ttcttaccga ccggatatcg aaatgaattt tgccgaaaaa
    15001 ataggaaaca aaacagtatt aaaagacgaa ggtttcgaaa ttgaagagct aaatacagaa
    15061 atccgattag ctgaacatct ttaaaaatct cttatgagca gtttttaaat aaaaaataac
    15121 ttggataacg atcgattttt gatcattgtc caagtatttt tttggtatac tagtaccagt
    15181 accatttcta aggagtgagt agatgcggat aatatcagga aattatggcg ggcgccgttt
    15241 aaaaagttta gcaggggcga acaccagacc aactacagat aaagtaaaag aatccatatt
    15301 caatatgatt ggaccatatt ttgatggaga gacagtattg gatctgtttg caggaagcgg
    15361 tggtttagct atcgaagcag tatctagagg ctgcagtcat gcggtatgcg tagacaaaaa
    15421 ttaccaagcg ctaaaaatca tcaaagaaaa catcgaaatt acgaaagaac cagagaaatt
    15481 taccacaata aaacttgatg cggacaaagc aatcgaacgg ctagcagcgg agaaacaaca
    15541 attcgactac ttgtttcttg atccgccata tgcaaaacaa aaaataatcg atcaaatcca
    15601 taaaatggaa gaactgggac ttttttctaa aaccgcagtt atcgtatgcg aaacggataa
    15661 aacagtcgaa ttgccagaaa ctgtggctgg attccatcaa ataagaaaac agatttatgg
    15721 cataacggcc gtaactatct atagaaaaga gggaaatcaa tgaaacgtgc attattccca
    15781 ggaagttttg atccttttac aaaaggtcac ttagatacgg tggaacgtgc ggctaaatta
    15841 ttcgatgagg tagtaattgg tgtttttatc aatacttcaa agaaaagttt gtttccccca
    15901 gaagaaagaa tgacattgat cacaaaagct gtttctcatc ttcctaatgt aaaagtcatg
    15961 catcaagaaa atcaattgac ggtagaaaca gcaaaagaga ttggtgcaga tgcgttgatc
    16021 agaggcatac gaagtatcaa agactttgaa tatgaacgag agattgctca gatgaatcat
    16081 catctatatg atgaattgga aactgtcttc ttattagcaa aaccagaata tagtcacgtg
    16141 agttccagta ttttaaaaga agtactgcac tttggcggag atgtgagcag ctacttaccg
    16201 ccagtgatca acgaagcttt agctggaaag cgtgaatcaa atgaaagcta aacaatggtt
    16261 gaaaacagta ctgattatca tatttggaat gttgatcatt ggatttgtag tagttccctt
    16321 gccgtattat gtagaatcac cgggagccac gatcgatctg aaagaactaa ttacggtcaa
    16381 tgataaggaa gataaaggtt ctgggtcttt ctctcttacc tctgttggta tcaggcaagc
    16441 tacaggtttc ttagctttaa aagctaaaat gacccctttt gaagaattga tcagtgaaga
    16501 agaattgact ggtggggcga caaatgaaga atataatcaa atgcaacagt attatatgga
    16561 ttcttcccaa aatgcagcaa tcgaacaagc gttaaaactt gcagacaagc cttataaaat
    16621 ggaattcaaa ggcgtatatg tcatgagtgt agagaaaaca tcgaatttct ttggcaagct
    16681 ttcagttgga gatacagtaa caaaagtaga cgggaaaagc tttcaaagta cagaagcatt
    16741 tatggattat gtaaaaagcc aaaaagttgg acaaacaatc gaaatcagtt atttgcataa
    16801 tggtgaagaa aaaacagctt ctggtgatct gatcgagtta ccaacagata aaaaggcagg
    16861 aataggtatc acgcttactg accataccga gatcgaatca gatacagatg ttcgtattaa
    16921 ttctggctct atcggtgggc catcagcggg attgatgttc actttagaaa tctatgaaca
    16981 agtcactggt aaaaacttgc gacatggtaa gcaaattgca ggaactggaa cgatcaacag
    17041 tgaaggagaa gttggccgaa tcggaggaat cgataaaaaa gtggcaagtg cggataaagc
    17101 tggcgtagag attttctttg caccagatga tacgatttca gacgatgtta agaaagaata
    17161 tcctgaaata aaaaccaact acgaagaagc aaaagctgcc gctaaaaaat tgaattcctc
    17221 aatgaaaatc gtaccagtaa aaacagtaca agaagcgctt gactatctag agaaaatgaa
    17281 atgaaactaa ataattaaag aggggttgtg acacgagttc tgtcacaacc cctctttttc
    17341 gtcaaaaatc ttcacgataa aaataaaaac aaaagaaaaa attgacgtaa ttagatgttg
    17401 aagggacgat tttgccagag ctcactgttt cttcaatttt aaatgaaagg agtaatgaat
    17461 gatttttact ggtcattctt tgaaaaaaga acaatgatag aaaaaatcag caagaaaata
    17521 ctagcttcac ccaaattgat catttggatg tctatgatag ttcttatctt ttgtatcgca
    17581 agtggttttt tattatcaaa tttgctattt tatagagaaa cagaagagac tgctgcaaaa
    17641 gaaatacttt cttcgtttag ttccagtgga gaagatccat cagctgaagg aaacgatgta
    17701 gaagaggatg ctcgtccaaa ggtcatgtac accgatatca aaggaagtgt gaaggagcct
    17761 ggtatctatt ccttttcttc agaagaacgg gtctatgatg tactaaagcg tgcaggtgga
    17821 ttattagaag aagcggatag cgatcgaatc aacttttctg cgaagataga agatcagcaa
    17881 gtcctatata tccctgctgt aggtgaagag ctgccagaac atcttgatca gttggcatcc
    17941 tcggaaggaa aacagcctac agcagattcc gagcctccca aaataaatat caatacagcc
    18001 actccatcag aacttcagca gattcctggg atcggatcag tcaaagcgca agaaatcatt
    18061 cgatttagag aagaaaacgg atccttccag aaagtggaag acctgcagga gatttctggt
    18121 atcggtgaaa agacagttga aaaactaaaa aacttcgtta caattaaata gaatgattca
    18181 aaatcgactg gcacgagcaa taaatggacg ctaatggata ctaagaataa tccaattcta
    18241 agagcttggc aggacgagga ggtagtattt ttggatagga aatagcttga ttgttcgcaa
    18301 agaaacatta aaaaaaccaa tgctgaatgt ctatctccaa aataaaatat ctgggattca
    18361 tattatgaat acggctgtca gcggcaataa cagtcaagct ctacgagaac gctttgccaa
    18421 agatgtactt agctatacag cagataaagt atttattctc attgggacga atgatctagc
    18481 agagcataaa caactttcta aagaaacgta ccaaaaaatc tgcagtggat gatagttcag
    18541 ctgaaaaaag actatagttc tgatcatgct ttctttatca cgccaccagc agtcgatgag
    18601 acaaaacaaa aatatcggaa taacaagttg atcaaagaat atatctctgt tctcacagaa
    18661 gtagcaaaac gccatgattg tgaagtatcg atttttatac ggaatttctt gaacaaatgg
    18721 attcgaagaa tttttataat gggctaatgg atgacggact tcatttcggc agtgaagggt
    18781 aggatctttt gtcagacagg atgattcgtt atatttaaag aatattttgt ttaagcaatg
    18841 agtacttttt aatcaacttg aaaaaacgga ggaatataaa acatgttaga aaaatcttca
    18901 aaagcatgcg atacgaataa agaaaaagtt actgataagt cacataatga acgcattcct
    18961 tgggaccagt actttatggc tcaggcagta ctgttatctt tacgtagtac gtgtacacgt
    19021 ttagaagtag gagcgacact cgttcgagaa aaaaggatca ttgctggcgg ctataatgga
    19081 gcagtcagtg gagatgtcca ctgcatagat gaagggtgtt acgtcgtgga tggacactgc
    19141 ttgcgaacga tccatgcaga gatgaatgca ctcctacagt gtgcaaaact tggtatcccg
    19201 acggaaggtt cagaaatcta tgtcacacat tttccatgtt tggcctgtac aaaggctttg
    19261 ttgcaagctg gtgtgaaagg gatcaattat ctccatgact atcgaaatga tccttatgcc
    19321 caagcgttga tcgaacaatt aggtgtatct gtacaccaag ttgctttaga ctcacagcat
    19381 ttttcaaaac ttcaaaatga attaacagga aaacatgtct agcgttttag aaaggcatgt
    19441 tcgaaatcgg ttgatttttc cggcaattac gtcgatgatt atctgtttgt tgtattacac
    19501 tgacaattgg ggttatcgtt tgcttcttgc cggaattttt ttatggtttt cgctaaaaga
    19561 aggacgtttt atcgcgtact tgtgtatttt gtgtagcata acggtatcca tttcttatag
    19621 gctacaagag tattcatttt tagaggaaaa aacaacatct gcgcaaatta ctgtatttac
    19681 agatacgatt cgtgcaaatg gtaatttttt gacaatggaa ggaaggttat cagagaataa
    19741 acaaaagctc cagctttctt atcaagtaaa cacagaagca gaattgaaaa catggcttaa
    19801 cttccgagga aagggaatag aattgactgc tgaaggcgag ttcatctctc cagcaaaaaa
    19861 gcggaatctt tataattttg accaagaagc ttacttcagg aaccatagaa tttctggacg
    19921 gttccaaatt caaaagattg aacagatcac catgcaaaat cactggcgaa actggcttca
    19981 gagaaaaaga ggatctttta tctcctttgt ccaatctgaa tttccgcaaa aa
//
LOCUS       DAYYNQ010000054        19958 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000054 DAYYNQ010000000
VERSION     DAYYNQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.054"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1186)
                     /locus_tag="ACHBJ1_001756"
     CDS             complement(203..1186)
                     /locus_tag="ACHBJ1_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM9430692.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1191..1838)
                     /locus_tag="ACHBJ1_001757"
     CDS             complement(1191..1838)
                     /locus_tag="ACHBJ1_001757"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9430693.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1923..2555)
                     /locus_tag="ACHBJ1_001758"
     CDS             complement(1923..2555)
                     /locus_tag="ACHBJ1_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM9430694.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2571..3122)
                     /gene="coaC"
                     /locus_tag="ACHBJ1_001759"
     CDS             complement(2571..3122)
                     /gene="coaC"
                     /locus_tag="ACHBJ1_001759"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM9430695.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3119..3856)
                     /locus_tag="ACHBJ1_001760"
     CDS             complement(3119..3856)
                     /locus_tag="ACHBJ1_001760"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM9430696.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3853..4842)
                     /locus_tag="ACHBJ1_001761"
     CDS             complement(3853..4842)
                     /locus_tag="ACHBJ1_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM9430697.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5100..5642
                     /locus_tag="ACHBJ1_001762"
     CDS             5100..5642
                     /locus_tag="ACHBJ1_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM9430698.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5659..6744
                     /locus_tag="ACHBJ1_001763"
     CDS             5659..6744
                     /locus_tag="ACHBJ1_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430699.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6756..7562
                     /locus_tag="ACHBJ1_001764"
     CDS             6756..7562
                     /locus_tag="ACHBJ1_001764"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430700.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7563..8390
                     /locus_tag="ACHBJ1_001765"
     CDS             7563..8390
                     /locus_tag="ACHBJ1_001765"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430701.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8387..9460
                     /locus_tag="ACHBJ1_001766"
     CDS             8387..9460
                     /locus_tag="ACHBJ1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430702.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9888..12584
                     /locus_tag="ACHBJ1_001767"
                     /pseudo
     CDS             9888..12584
                     /locus_tag="ACHBJ1_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            12649..13149
                     /locus_tag="ACHBJ1_001768"
     CDS             12649..13149
                     /locus_tag="ACHBJ1_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9430703.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            13161..13655
                     /locus_tag="ACHBJ1_001769"
     CDS             13161..13655
                     /locus_tag="ACHBJ1_001769"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9430704.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13676..14485
                     /locus_tag="ACHBJ1_001770"
     CDS             13676..14485
                     /locus_tag="ACHBJ1_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9430705.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14482..15303
                     /locus_tag="ACHBJ1_001771"
     CDS             14482..15303
                     /locus_tag="ACHBJ1_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9430706.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15354..17453
                     /locus_tag="ACHBJ1_001772"
     CDS             15354..17453
                     /locus_tag="ACHBJ1_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM9430707.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17450..18568
                     /locus_tag="ACHBJ1_001773"
     CDS             17450..18568
                     /locus_tag="ACHBJ1_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM9430708.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18607..19485
                     /locus_tag="ACHBJ1_001774"
     CDS             18607..19485
                     /locus_tag="ACHBJ1_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9430709.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19631..19828
                     /locus_tag="ACHBJ1_001775"
     CDS             19631..19828
                     /locus_tag="ACHBJ1_001775"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9430710.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         6092 a         3471 c         4274 g         6121 t
ORIGIN      
        1 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
       61 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
      121 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
      181 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
      241 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
      301 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
      361 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
      421 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
      481 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
      541 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
      601 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
      661 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
      721 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
      781 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
      841 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
      901 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
      961 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
     1021 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
     1081 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
     1141 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
     1201 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
     1261 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
     1321 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
     1381 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
     1441 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
     1501 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
     1561 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
     1621 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
     1681 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
     1741 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
     1801 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
     1861 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
     1921 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
     1981 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
     2041 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
     2101 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
     2161 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
     2221 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
     2281 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
     2341 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
     2401 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
     2461 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
     2521 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
     2581 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
     2641 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
     2701 gatctcaatg tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg
     2761 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
     2821 tcgtctgcaa tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
     2881 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
     2941 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
     3001 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
     3061 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
     3121 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
     3181 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
     3241 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
     3301 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
     3361 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
     3421 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
     3481 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
     3541 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
     3601 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
     3661 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
     3721 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
     3781 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
     3841 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
     3901 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
     3961 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
     4021 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
     4081 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
     4141 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
     4201 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
     4261 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga
     4321 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
     4381 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
     4441 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
     4501 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
     4561 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
     4621 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
     4681 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
     4741 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
     4801 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
     4861 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
     4921 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
     4981 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
     5041 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
     5101 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
     5161 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
     5221 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
     5281 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
     5341 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
     5401 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
     5461 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
     5521 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
     5581 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
     5641 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
     5701 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
     5761 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
     5821 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
     5881 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
     5941 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
     6001 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
     6061 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
     6121 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
     6181 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
     6241 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
     6301 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
     6361 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
     6421 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
     6481 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
     6541 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
     6601 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
     6661 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
     6721 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
     6781 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
     6841 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
     6901 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
     6961 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
     7021 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
     7081 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
     7141 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
     7201 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
     7261 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
     7321 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
     7381 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
     7441 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
     7501 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
     7561 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcctatatg
     7621 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
     7681 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
     7741 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
     7801 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
     7861 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
     7921 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
     7981 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
     8041 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
     8101 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
     8161 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
     8221 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
     8281 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
     8341 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
     8401 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
     8461 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
     8521 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
     8581 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
     8641 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
     8701 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
     8761 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
     8821 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
     8881 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
     8941 tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa
     9001 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
     9061 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
     9121 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
     9181 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
     9241 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
     9301 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
     9361 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
     9421 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
     9481 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
     9541 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
     9601 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
     9661 taaatctaag attgaaaaag ttacagaagg ataaagtaat tggaaactat gcaaatatac
     9721 ttaacccttc tgtaatctat taataaaact aatatatgaa tccagttcaa tatttgtagc
     9781 tatgaaaaaa cataaaaatc atattagata aaaactaaat tggtagataa attattttgt
     9841 cagatatctg aggcaaatat tcgactattt gtgtttcaaa gtaaaaattg gaatacaaat
     9901 atagaataga catattgcag ccgatagttt attatgcttt tagacacatt aaactggagg
     9961 aaaaaatgaa aaagagcgct aaagatcgag tttttgatga gctgatacga ctaacaggga
    10021 atatgtcttc tgtcactgcc gagaaaattt cgaagaatct aggtatctct cgtcagaatg
    10081 ccagccatta tttgacgaga ttagtggaag ataaaaaggt cgagaaatta ccgggaaagc
    10141 ctgttttgtg gaagccttta gatgaatatg cggttgtaga taatacggag caaataaacg
    10201 aagcctttca ttctgtagtt ggtcatgacg gaagtctgag agaggtcatt caaaaatgta
    10261 tagccgctgt caaatatccg cctaacggac tcagtgtatt aataaatgga gccacgggtg
    10321 ttggaaaaag ttttttagcc acaaaaattt ttgaatacgc tatccatgag caaattattg
    10381 aaaaagatgc accatttgct atcttaaact gtgccgatta tgcggacaat cccgaactgc
    10441 tttcggcaac actgtttgga tacaagaagg gggcctttac gggggctgaa aaggataccg
    10501 atggtttatt ggcaacggcc aacaacggct atctttttct cgatgaggtc catcgcctat
    10561 cgaaagaaaa tcaagaaaag ctctttctat ttattgatac tggaaactat cgtccagtcg
    10621 gtgaaaatgt aaattggcat tctgctaagg ttcgttttat tttcgccacg acggaaaaag
    10681 gtgaaaatta tttattagac acctttgatc ggcgtattca aattagtgtt atgttgccaa
    10741 cttttgaaga tcgccccatt cgagaacgat tagagctgat tcaattattt tttcaaaatg
    10801 aagcggatgt tttacaaaaa gatattattg tttcaagaga agccttgtct ttaatgttat
    10861 ctcatccctt tagtgggaat attggtaagc tgaagaatat cattaaaatc agttgtgcgg
    10921 atgtgtatag tcgaacacaa gaagaaccat taacgattgg gaaaaatgag atcttgattc
    10981 agttaaatat gctggaatcc gaagtagaaa gcttgccgat tgctagttta ttgataaaga
    11041 aaaatcaaaa gataaaaacg gatcgaatca aaccgtttga ttatgattcg attttggaaa
    11101 aaattactca gctgataaat gaaggtaatt tcaatgaaaa tttttcttcg ataaagaatg
    11161 aagtacagca tttggtgcat cacattgata aggattcttt tttatcaatg agtagaaaat
    11221 tgatgattca gatgtttaga aaggtctggg tatccgtttt aggaaaaacg tacggattga
    11281 cgacgagtgg acctgttgca gaaattatta gcagatccta tgtcatttcc ccaagctata
    11341 agacagactt aaccgggata aatcgtacat tagctgcaaa tctacctcgg acgacttact
    11401 tatgtaatca gttcttggag catttaccac cgctctcaga aaaggacgag aactttctaa
    11461 gactgcttat tgtcgtgact ttatctgatt atgtagatga atccatcgag ctgaagggcc
    11521 tgttgcttgc tcacggagat agtacggcta gcagcattca agcagtagtg aatcaattat
    11581 gcggcaatta tgtttttgaa gcgttggata tgccagtaga gagtaatgtg tcagagattg
    11641 tcgaatcgac aaggaatttc gtgaaacggc aagtgaatac agatagtcta gtgctgatcg
    11701 tagatatggg ctctttaaat caaatttaca cacagatcaa gaacgattta aaaggggagt
    11761 tacttcttct gaataatttg acgacttcca ttgcacttga tattggcttg aagatgacga
    11821 tgaatgcgcc atttaaatag attgcggagg atgcagcaga caagtattca atcaatgttc
    11881 aatactttga aggcttttct caaagtagca acattatcat ttcctgtatg tcgggtttgg
    11941 ggatttcgga caaattgaaa gatgtttttc gtcatcattt atcggatgaa tccatcgaag
    12001 ttttcacgaa ggactacaga gaactacgtg aattaatcga ccaaaatgat gaaacttatt
    12061 ttgacaagac aaaattggtc atcacgactt cggatcttcc acgttccttt tcaattccta
    12121 atattaatgt ttatgacatc ttagacggtg atggagcagc caatttggag catattttga
    12181 gtcctgaaat gaagccggca gaatttaacc gcttgattca ggagctagta aaattctttt
    12241 cgttggaagg aattgcggac cgtttgaact ttctgaaccc aacgatcgtg atgagtgaag
    12301 tggaaacagt catttctaaa tatgaaaatt actatcattt cactttaaat ggccgtgtaa
    12361 aactaaatct ctatatgcat acggcattga tgatggaacg tttattcttg gctcggacag
    12421 aaaaatctga agaaccgaat tatgatttga aaccagatga agatgaattt tattatgttt
    12481 ctagaagtat ttttcaatca atggagatga aatataattt taaagtcaat gcttatgagc
    12541 tttcgctgct ctatgaatta ttaggaccgt atattcaaaa ataaagtgtc taaaaaagtg
    12601 tctaatctgt tttttttagg ttagacactt tttttaatgt gaattaacgt gattaaacca
    12661 atgtttttaa aagttggcac aggaattgct tatataaaag tgaacagaaa gaaaggaatt
    12721 aaccatatga agaaaattct aatagcgact cacggtcact tagcttcagg ctttcttagc
    12781 agtattcaat tactgacggg gaaggttcaa gagattacgg tgattaatgc ttatgtggat
    12841 gagtgtgatt ttgaacagga gttgaatgta tttgtaaacc aggtgaacga gcaggatcag
    12901 atttttgttt ttaccgattt atttggcgga agtgtcaatc agaagattac caaaaccttt
    12961 ttggagaaag agattgctgt aactattttg tctggcttta atctgccaat tctgctcgaa
    13021 atagtattgg caaccaagga attaacaaag gaggatattc aggaactggt aaataaatgc
    13081 caacaggaat taaagatgac cattcttgat gaggtacatg tagtaaatga agaggaagat
    13141 tttttttagg aggaagaaaa atgattaaag cagtgcgaat tgatgaacga ttaattcatg
    13201 gacaagtagc gatgacttgg acaaagagtc taaatttaac cgggttagta gtagcaagcg
    13261 atgaggcggc taccaacgat attcaaaaga tgacattgaa gatggctgca ccatcgaatg
    13321 tcaaagtcat tattaaaaca gttgatgacg cgattcattt gttaaaagat ccgcgggcga
    13381 aggacatgcg cttgatggtt ttagtcccta atgtcaaaga tgcggtaaaa gttgcgaaag
    13441 ctttcccaac ggaaatcgaa ttaatgaacg tggggaatgc tgggaaaatg acaccgccat
    13501 cggaagataa gaaaacctta tcaaaggaag tgatgttaac gacgtcagaa attcaagact
    13561 taaaagaatt agttgaactt tatccagata ccgtttttca gcctacaccg gcgatggaaa
    13621 aacgttcagc tatctctatt ttgagtgatt tttagaaaaa ggaaggagaa aaattatgta
    13681 tgatgcattt gtagtcgctt tggccgtttt tattggagtt gctggacatg aaatgttcgg
    13741 gatgtccatg ctgagtcgtc cgattgttgt tgctccgtta gcaggagcgt tgatggggga
    13801 tttgcagacg gggttgattg tcggggcgtc actagaagcc atctttatgg gtgtcgtcaa
    13861 tatcggaatc tctagtacgg cggagccttc tttggctgct gggctggcta cagcgttcgc
    13921 aatcaaaatg ggaggaaacg tggatgcgat tattcccatt gtctttccac tagcagtatt
    13981 aggattacag ttaatgaata tgattttttc ttttgtatgt ggaccgatgg ctgctaaatt
    14041 tttcaaattt gcaaaatctg gtgatcaaaa aggaatcatc aatctgcact tctttatgtg
    14101 gtttgtccac tatgcgcttt atgctcttat tccattcttt gccgttttat ttggagcaga
    14161 tgttgtcaaa agcgtcttgg ataatattcc agaggtcatc atgaacggat taacagtcgc
    14221 tggaaatctg ctgccggcag ttgggatggc gatgctgtta cggatgcttt ggagtaaaaa
    14281 tattgctgtg tggtttttct ttggggccgt tgttatggcg tatctcgagt taccattgat
    14341 tgcaattgcc gcagttggaa cgattattgc aattgttata gcccaaagag atctacaact
    14401 aaaaaatata tctgttcatg cagttcaaag cgatgctgct gtaaatgcaa cgcttgacca
    14461 agaagaggag gatttctttc aatgaaatta gctgatggaa tttctaaaga tgagaaaaaa
    14521 ttggttcgga agatgttttg gcgctcgatg actatgtatg cttcggtgaa tccggtgacg
    14581 atgggcggcg gtggtttcgc ctattctatg atgcccttca ttaatcattt ctacaaaagt
    14641 gaagaagatc gagctgcggc gctggaacgt catacacagt atttttcgac gaccattcct
    14701 tgcgcgtcct ttgtcatggg gattgcggca tcgatggaga aggaaaattc tgaaaaagct
    14761 ggttttgacg tggaaagtat caacgcgatc aaaacgagtt tgatgggacc attggctgga
    14821 attggcgatt cattgttctg gggagtttgg cgtgtcgtta ccgcgggact agcgatcaac
    14881 ttagccaatg ccggaaatat tttggcacca attatctttt tagttatgtt caatatacct
    14941 aattacttga tgcgatacta tggcggcgtt ttaggctatt cattaggctc taaatatatt
    15001 gaaaagctgt atgcgaacgg gttaattgaa attctgacaa aagctgctag tatcttgggt
    15061 ctgatcatgg taggtgcaat gacgatcagt atggtcaaat tcaataccgt tttgagcttt
    15121 tcaatgaagg gcgaagaact aatgaatgtc caagaaatcc tcgatagtat ttttaaagga
    15181 attgtaccgt tattggtaac attgagctgt ttcaaattac tagataaaaa agtcggcgtc
    15241 atcaaaatta tttttggaat ggtcgtcttg ggaattttat tgtcattact tggaatcgca
    15301 tagtaggaag ctgcagctac taggctgcag tttttttagc aaggagggaa aagatgacta
    15361 ttaatgaagt aatttattca cccaaacacc cattttttaa tgctgagtta aacgacggaa
    15421 atgtgactac ttcaaacgaa tacagctact atcggaatat tcctgatgta aagggattga
    15481 ccttcgatgt aagtaatcaa aagacattag caaaccttga tgtttgcggg acgattcaaa
    15541 cgttgacctt ttatcgagaa aatttactaa ctgaggaaaa accaggtgtt tgggtcaata
    15601 aacagctctc tcaaaccaat tcgttatcct taaaggttga agtaaacggg attgtctacc
    15661 atttgtcaga atcggatcat cgtattgaag tcgatgtgat tcaagactgt ttgcctagat
    15721 atattcatca ttattcgacg tttaaggtca ttgtagtatc ctttgctccc atactggcta
    15781 ataagcgttt aagtatgctt gttcaacagg tttacattgt gaatgaaaca cacgaaagcc
    15841 ttcaagtttc agtgaaagat gtgccttcgt atcaagagaa gtattcggat cagcaaaatg
    15901 tgttgatttc tcaaaaagga aataaggaca cgatcgcaca acaggaagtg gcaagcttcg
    15961 ccattgcttt tgtggaccca aatgcttttg aagaagtaat gacttttcag gaatctgata
    16021 tcgaaaaatg gctgaaggaa acattgaact attttgctca gctatttggg cagctgtcaa
    16081 tgccaaacga tagagttgtc catctctatc gtcgtgcact ttatcaaagc ttttctagtt
    16141 tcggtatgaa tcgacaggga caggtcgtcg gcagcaattg gggcagctat cctgctacta
    16201 atcgaatttg gaataaggat atgtattacg catcattgcc attcaccatg tttgaaccgg
    16261 agttatgtca aaaaacggtt ctatggtttg atcaatatgg cgttaagttt cctggttcaa
    16321 aatttacagg aggtatcaat cattctctga gtaattcgtt atccagtgcg ttactggcta
    16381 gtctttattt tgaacatact ggggatacaa aattctttga gcggtgtccg caggttctac
    16441 tgaatgcttc aagaattatt gaggacatat tagctcaacg tcggtcaggg gaaccaatgc
    16501 tttttccatc tgtttggtta tcagacgcct ttgcacttgg aaagtatcat accggcagca
    16561 atctttgtct ttggaaggcc tgtattggac ttgcagaaat ttttgaagcc ctagaacaac
    16621 tgagtcttag caagcgatac aaaaacattg cctgtaaact aaaggaatca atttgtaaac
    16681 aaatgactat cgatggtcct tttggtgagc aatttttgga gggaatcggt gatgaggaga
    16741 aaacaaccta ttcagtgaag cactatcaaa aaccgattct tgagcaaggg ttaatctttt
    16801 taagtgatgt gattgtggat ggaaaaatta atttattgat gcatgatgga gaagaatcag
    16861 acactacgtt gatgcctttt tatcaatttt tagataataa agatcattta tatcagaaca
    16921 ccatggcctt ttctgctagt tctttcaatc caacctatag cgaggaaatc aaaggcatca
    16981 cttggggatt agaatccggc gccacttttc ctggctttat cactgtttta atgagcgagc
    17041 tgcacaaccc tcaagctttc gtaacccgat tagaagaact aatttgtttg gcagatttag
    17101 acggttcgtg gtggtggtgg ccgtatcgct tagaggctca acaaggagaa gtcgtgcgag
    17161 actttggctg cggcaaatgc ggctgggcat cgggtctttt tgtcagccta atgatcagtc
    17221 aatatttcgg attgaaattc aaaaaaggag tattgcaaat tgatcctgtt gataatttaa
    17281 cgttttcttg gaagaatttg aagttcggtc aagccttcat ttcgattgaa tgtacgcaat
    17341 ccgagttgag tatttcaaat ttgtcagaaa agccattgaa aataagtgat acgaaaaaaa
    17401 ttcttcacgt agaaaaggga aaaacgattc acattcaaag gagaaaaaga tgagtatact
    17461 aaaaaatatt acccaattta aaaaaatcca gagtcgagca atcactcccg aaaatccaac
    17521 aggcgagaaa ggtaaagctg cgatgttgcc aagcgagcta ggaccaagcc gcaaaggtcg
    17581 tggcgcaatt ccgctgactt ctggtgaaga aactgtgatt gcggatatta ctggaacggg
    17641 aataattcgt catatgtgga tgacgattcg tgatcgaaca gaatttggca gttttgtttt
    17701 acgtgattta atcttaagaa tttattggga tgggtcggat agccctgctg ttgaaagtcc
    17761 tttaggggat ttcttttgta atgggtttgg agagcgttgc gaggtgaact cgttgccgat
    17821 agtggttaat cctacagggg gctttaattc ctatttcgaa atgcctttcc aaacatccgc
    17881 taaaattacg atcgagaatt tacatccgaa ggatatgtcc tcattttttt atacgataaa
    17941 ttacgctttg gtggacgaat tggaagaaaa tagcctatat tttcacgcaa attggcatcg
    18001 ggaacgcatt accaaattac aggaggatta taagttaatt gatcgattaa agggtcaagg
    18061 ctattacgtg ggtacttacc ttgcgcttac ctgcctagaa cgttattggt ggggcgaagg
    18121 cgagtttaaa ttctacattg atggggatag tgagtatccg accgtaacct caacaggttc
    18181 agaagattat tttggcgggg cgtgggcctt tcatgagtac gatgagttcg gacgtccaca
    18241 tgcgaaaaac tattcaacag cctttttagg gtatccttac caatcaaata gagatgccac
    18301 aagagatcgt tttgaaacgg gaaaattgaa tgccgttcat gcctttggtg acgacgcaat
    18361 gccacgacat ggtctttatc gttggcatct tcaagacccc atctgtttta ccgaagattt
    18421 atcggtcact ttacaacaaa tcggcaacga tgatgttcgc ttatttgagc ggcaagacga
    18481 tgttgctagt gtcgcttact ggtattcaac agaagcttca ggaaatacta aacgtcattt
    18541 cactgatcga gaacgcttgc cgcgttaatg aagtgttttt cacatattcg aagggaggaa
    18601 cctatgatga tatatggggc aattgaagct ggagggacta aatttgtctg tgcagtcggt
    18661 actgaggaat taacgatttt ggaaagagtc agttttccca caacaactcc agttgaaaca
    18721 atgaagcagg tcattgattt ttttaaacct tttaaggaac aattggcagg catcggggtc
    18781 ggctgctttg gtccaattga tattcaatgc aaatcaacga gtttcggtca tatcacatca
    18841 acaccaaaac ttgcttggca aaatttcaat tttgtaggaa cgctaaagca atatttcaat
    18901 attccgattg aatggacaac agatgtcaat gcggcttgct atggtgaata tgttgctggt
    18961 tctggcaaag ggttagcagg tgttgcatac ttcactgtgg gaacaggtat cggaggtggt
    19021 gccttgttca atgggacttt cgtggaaggg ttcagtcatc cggaaatggg gcatatgttg
    19081 atcaagcgac atccaaagga caatttttca ggaaactgtc ccttccacca agattgttta
    19141 gaggggatgg cagcaggacc agcgattgaa aaacggttgg gtgtaaaagg ccagaattta
    19201 ttggctgacg attcgttttg gcagattgaa gccttttacc ttgcccaatg tgcatacaat
    19261 acaacactga tgttttcgcc agatagaatt atttttggcg gtggtgtaat gaaacaagaa
    19321 cacatgaaga aaaaagttca ggataaattt gttgagctta taaatggata tgttgaaata
    19381 ccaccaattg atagctacat tattacgccc gaattaggtg ataatgccgg aataatcggc
    19441 ggattagcat tggccagaaa agcggtaaga aataagcaac cataattaat gaatgtttca
    19501 ttgatactca tgtcaaaagt atttaaccga gtaatcaaat gatcaaatga agttggataa
    19561 agcgaaaatg aattcaaata aaaaggctct aagtcaaatc gtgttgtttt cattaaatca
    19621 ctgaaaggaa atgcttctgc gtttcctttt ttctttttgt tgtgcgctgt ataccttgag
    19681 gatattcact ggtggcgtac acttaaatag taatatattg tgttacttta tgtctgtggt
    19741 aatagttata gttgcttacc aatgtatgtt catttttcga aatgttgact tcataatcct
    19801 tggcttaatg tttgaatttt tttggtgata gattttttca gccgtataaa ataaatcgta
    19861 attctattat agagagttgt gattataaaa agatagcaag aaaaaaaaag aaagtttcta
    19921 tacattttaa atattttatt cctatttcta ttgctaaa
//
LOCUS       DAYYNQ010000055        19629 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000055 DAYYNQ010000000
VERSION     DAYYNQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.055"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBJ1_001776"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACHBJ1_001776"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM9430711.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACHBJ1_001777"
     CDS             complement(1172..1867)
                     /locus_tag="ACHBJ1_001777"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM9430712.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACHBJ1_001778"
     CDS             complement(1880..2596)
                     /locus_tag="ACHBJ1_001778"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM9430713.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACHBJ1_001779"
     CDS             2926..3534
                     /locus_tag="ACHBJ1_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM9430714.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACHBJ1_001780"
     CDS             3859..4182
                     /locus_tag="ACHBJ1_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9430715.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACHBJ1_001781"
     CDS             4364..5734
                     /locus_tag="ACHBJ1_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM9430716.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACHBJ1_001782"
     CDS             complement(5864..7165)
                     /locus_tag="ACHBJ1_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM9430717.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACHBJ1_001783"
     CDS             complement(7519..9510)
                     /locus_tag="ACHBJ1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM9430718.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACHBJ1_001784"
     CDS             complement(9654..9920)
                     /locus_tag="ACHBJ1_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9430719.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBJ1_001785"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACHBJ1_001785"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM9430720.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBJ1_001786"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACHBJ1_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM9430721.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACHBJ1_001787"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACHBJ1_001787"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM9430722.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACHBJ1_001788"
     CDS             complement(14080..14601)
                     /locus_tag="ACHBJ1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM9430723.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACHBJ1_001789"
     CDS             complement(14604..15446)
                     /locus_tag="ACHBJ1_001789"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM9430724.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACHBJ1_001790"
     CDS             complement(15439..16164)
                     /locus_tag="ACHBJ1_001790"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM9430725.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACHBJ1_001791"
     CDS             16340..16636
                     /locus_tag="ACHBJ1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430726.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACHBJ1_001792"
     CDS             17204..18796
                     /locus_tag="ACHBJ1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM9430727.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACHBJ1_001793"
     CDS             18809..19549
                     /locus_tag="ACHBJ1_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9430728.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DAYYNQ010000056        19482 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000056 DAYYNQ010000000
VERSION     DAYYNQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.056"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..208
                     /locus_tag="ACHBJ1_001794"
     CDS             <1..208
                     /locus_tag="ACHBJ1_001794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430729.1"
                     /translation="ANNRTKKQTRLFLAGISFLKELKREKMIFIIKEFLSFSLEKISV
                     TMENRAKKNQSFSLYIILPIYRNC"
     gene            240..1142
                     /locus_tag="ACHBJ1_001795"
     CDS             240..1142
                     /locus_tag="ACHBJ1_001795"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM9430730.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            1355..3130
                     /locus_tag="ACHBJ1_001796"
     CDS             1355..3130
                     /locus_tag="ACHBJ1_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM9430731.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3196..4782)
                     /locus_tag="ACHBJ1_001797"
     CDS             complement(3196..4782)
                     /locus_tag="ACHBJ1_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM9430732.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            5033..5257
                     /locus_tag="ACHBJ1_001798"
     CDS             5033..5257
                     /locus_tag="ACHBJ1_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM9430733.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5398..7149
                     /locus_tag="ACHBJ1_001799"
     CDS             5398..7149
                     /locus_tag="ACHBJ1_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM9430734.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7149..8933
                     /locus_tag="ACHBJ1_001800"
     CDS             7149..8933
                     /locus_tag="ACHBJ1_001800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430735.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9259..10110
                     /gene="licT"
                     /locus_tag="ACHBJ1_001801"
     CDS             9259..10110
                     /gene="licT"
                     /locus_tag="ACHBJ1_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM9430736.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10206..12086
                     /locus_tag="ACHBJ1_001802"
     CDS             10206..12086
                     /locus_tag="ACHBJ1_001802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9430737.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(12126..13028)
                     /locus_tag="ACHBJ1_001803"
     CDS             complement(12126..13028)
                     /locus_tag="ACHBJ1_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9430738.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13259..14662
                     /locus_tag="ACHBJ1_001804"
     CDS             13259..14662
                     /locus_tag="ACHBJ1_001804"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9430739.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14662..16095
                     /locus_tag="ACHBJ1_001805"
     CDS             14662..16095
                     /locus_tag="ACHBJ1_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM9430740.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            16240..17166
                     /gene="trxB"
                     /locus_tag="ACHBJ1_001806"
     CDS             16240..17166
                     /gene="trxB"
                     /locus_tag="ACHBJ1_001806"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM9430741.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17380..19107
                     /locus_tag="ACHBJ1_001807"
     CDS             17380..19107
                     /locus_tag="ACHBJ1_001807"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM9430742.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19122..19472
                     /locus_tag="ACHBJ1_001808"
     CDS             19122..19472
                     /locus_tag="ACHBJ1_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM9430743.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT         6279 a         3419 c         4246 g         5538 t
ORIGIN      
        1 agcaaataat agaacaaaaa aacagacaag gctgtttttg gcaggaattt cttttttaaa
       61 agagttaaaa cgtgagaaaa tgatctttat tataaaagag ttcttaagct tttctttaga
      121 gaaaatttca gttactatgg agaacagggc aaagaaaaat caatcatttt ctttgtatat
      181 catcttacca atatatagga attgttagta acaattgcct ttcaaggagg aatccatcca
      241 tgttattagg ctcacacgtc agcatgagcg gaaagaaaat gctgttagga gcagcagcag
      301 aagcagccag ttatggatct tcgactttta tgatctatac aggcgcccct caaaacacta
      361 gaagaaaagc catcgaagag atgaatattg atgccggaaa agcgtacatg gcagaaaacg
      421 gtctaacaaa tatcgtcgtt catgcccctt atatcattaa tttggggaat acgatcaaac
      481 cagaaatgtt tccttttgcc gtccaatttc tgagagaaga gattgttcgt gcggaagctt
      541 taggcgctac tcaaattaca cttcacccag gtgctcatgt aggggcagga acagaagccg
      601 gaattgctag aattattgaa ggcttgaatg aagttttgac aaaagaacag acggcacaaa
      661 tcgctttgga gacaatggca ggtaaaggta cggaaatcgg tcgaacattt gaggaattag
      721 cgcaaatcat cgatggagta aaattaaatg aaaaattatc cgttactttt gacacctgtc
      781 atacgaatga tgccggttat aatattaaag aagacttttc aggtgtaatg gaagagtttg
      841 ataagatcat cgggttagat cggcttaaag tactccatat caatgattct aaaaatccgc
      901 aaggttcgca taaagaccgg cacgccaata ttggttttgg aacaatcgga ttcgacacgc
      961 tgaataagat cgtccatgat ccaatgtttg acaatatttc gaaaatcttg gaaactccct
     1021 atgtcggcaa agataaaaag aaagcttacg caccatatgg aaaagaaatc gccatgttca
     1081 aagcacaaat attcgatcca gaagtgtttc ctgagttatt agcagaagaa gtgatactct
     1141 aagattttct atctgacagc agcaaaaaag gaccaggcac attgccttgt ccttttttgt
     1201 ttaagaaaca aaggagtact ttttgcagtc tttttcatca tgcatcttct cccccctctt
     1261 tttaaattta gacaaacaag gtatactgtt agaaaagtaa aaaaacgaaa agtttcacaa
     1321 gcaagtggca aaacgtgatt ggaaggacga aaagatgctg aacactttaa aacccttgat
     1381 caaccaacta aaaaagaaac atacatactt tattcttggc atagccctat tatttacaat
     1441 tattcgtttg tatttatata gtaaagcaat ctatggaaca gacatgtcgg caacctatga
     1501 tgaccagctg ctgattcgct atgctcaaaa tattttggac ggtaaatggc taggggagta
     1561 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     1621 actgcatctt tcttatgctg ttttgttcgg catgttcagt atttttgcca gtatattggt
     1681 tgcactcgcg tttaaaccga tcgtgaagaa caagtatctt cttttaagta tctattttta
     1741 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     1801 cctcgtgatt ccggctgttg agatcattat cgcttgttta ctagctattt attaccgcaa
     1861 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     1921 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     1981 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     2041 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     2101 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     2161 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     2221 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     2281 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     2341 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     2401 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     2461 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     2521 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     2581 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     2641 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     2701 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     2761 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     2821 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     2881 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     2941 cggattaata ctgacacaat tggtttttct tttcggtgtc tcgtggtttt gttcttatgc
     3001 gccagaaaaa aaggattatt ttttaagtgt ctacactggc gcaggcgtgc cattaacgca
     3061 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aggctttatc
     3121 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     3181 tggaaaaatc agtttctatt cgatttcttc taccgtttcc tgcaaacgct gttcatattt
     3241 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     3301 gattacataa gcgatgtgat cagccaatgc tagttgagca gaagtggctt gttcaccaaa
     3361 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     3421 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     3481 gctgttggtc atcatcagta aagggatcat cgaaaacggc aacgcaaaag ccaagaacac
     3541 ctgggattca ttcatcaagt tattcaaagc ggtatgttct tcgatcgttc cttttgtact
     3601 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     3661 tagccaaatc gggatacgca tatggatgaa gccttccatg atgacttgac cggtcaacgt
     3721 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggataa
     3781 gataccagat tttgctacag caatcaagat accattgctc atagctgatg gatcagataa
     3841 ggcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     3901 acccatgatc aatagcagtg cattgactag aaaagccatc gacaattgga tattagagtc
     3961 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg tcttggtgat caatcttccg
     4021 agtttgagaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     4081 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     4141 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     4201 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     4261 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     4321 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     4381 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     4441 acttgtgcgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     4501 tgccatatac tgtaaaagca tagcaatcag actagacatc aaaataactg acatcaataa
     4561 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     4621 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aacttttccc
     4681 ctttggtaca tcaactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     4741 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     4801 atttatctac gattacattc tacacctcaa aaagaaaaaa ttaaagaaaa atattcggta
     4861 tgcctaattt attttcgtgg cgcgtcttgt tagatgacga aagaaatttt gttctcctct
     4921 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     4981 ttagtaaaat aagtcattga gagcaattcg gaaatagaaa ggagagattt atatgttatc
     5041 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     5101 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     5161 catgttgcga tcaatgatga tgtcaatggg acaaaaacca tctgagaaga aaattcgcca
     5221 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaac gactttttac
     5281 gggctgattt tttcagtccg ttttttattt tgtaatttgt tagactattc agattattca
     5341 gcaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
     5401 tcgattttta gaaagttagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
     5461 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
     5521 atcattgatg agattgccga taaaaattta tcagtaaaga tcattttcgg gtggatcttc
     5581 ctattgatta tcgctgcttt cttacaatat ctttttcgtt atatctggcg gacgaatatt
     5641 tggggaagtg cagctcggtt ggagaaagaa cttcggcagc agttattcaa acattttaca
     5701 aaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcatgcaacg
     5761 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
     5821 gtgattacgg gcggaacaac gatcattgtg atgattttgt ttattgattg gcgcttaaca
     5881 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcgaaa
     5941 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
     6001 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
     6061 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttctagac
     6121 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
     6181 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
     6241 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
     6301 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa aacgcatatc
     6361 gtggaacaac tgggtgcagt gcagaccccg gtaaaaggcg agctgaatgt agctatttcc
     6421 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcaatccaa
     6481 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacaac gatcatcaaa
     6541 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
     6601 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
     6661 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
     6721 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
     6781 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
     6841 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactt
     6901 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
     6961 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
     7021 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
     7081 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
     7141 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
     7201 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaca acgttcatcg
     7261 ttgctatctt atttgcgttt gctctgtctg tgatcaatgt cctattgcca aggatcatcc
     7321 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
     7381 ttgctggtat gtacttattt ggagtcctag taaaaagtat catttggttt ttccaatggt
     7441 atttgtattc catggcttca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
     7501 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
     7561 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
     7621 ctgggatctt tgcagtcgtt tcttcattca ttgccatgtt tcaaatcaat ggagaaattg
     7681 ccttgtggac tttggtgttc ttgccgattt taggaattgt catctggtat taccagaaat
     7741 ttagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaat acaaaattga
     7801 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
     7861 aagaatttga acaagtgaac aatgagtatt tagacacgcg ctatgcgatg atcaaaacga
     7921 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggcgt
     7981 tgactttatt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
     8041 ttacgactta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
     8101 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
     8161 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
     8221 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
     8281 ctaatccggg agaaaccgtt gcattagtgg gacacaccgg aagtgggaaa agctcgatca
     8341 tcaatgtgtt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
     8401 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
     8461 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
     8521 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
     8581 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
     8641 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
     8701 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcgaaaatgc
     8761 gacaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
     8821 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
     8881 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag tagtggtata taaaagatgt
     8941 tgtgacagta gcattttgac ctaagcacta gagtgaaaat aaaacatagc ttcccatatt
     9001 tttcatctgt gagagttagt gtcgtagtta ccagatttaa aaaagacgac gtcagctagt
     9061 cagaagtgcc taatagcccc taagaaagcg ttgacaaaat cgtttgcttt cgataatata
     9121 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
     9181 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatatat
     9241 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataaca
     9301 agggatgaaa gagatcggga aatgattgtg atgggaaaag gattggcatt caaaaagaag
     9361 gtgggtgacc cgattcttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacgga
     9421 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
     9481 tgtgaaatca ttgaatatac aaaagaggct ctgcaagtcc atttaaatga ttctgtgtat
     9541 atttcattga cggatcactt gtatacagct atcgaacgtc aaaaaatggg gatctttgtg
     9601 ccaaatgtat tgctgtatga tatcaaacaa ctgtttccta aagagtatgc cattgggaaa
     9661 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga cgaagccgga
     9721 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
     9781 ctgtttacca caacaataca ggatatttta aatatcgtcc gctattacta taagattaca
     9841 tttgatgaag attcagtcca cttttttcga ttcgtcacac acctgcgttt ttttctgaca
     9901 cgtgtcctag atggagcgaa atcagaagat ggagaagttt ctgaggaatt gttccaagtt
     9961 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttaacc
    10021 ggggctttcg actaccagat gtccacggat gaaaaaatgt atttaacgat ccatactgcg
    10081 cgtttagtgc atgagaatcg ttgtaaataa ataaaaaagg aggacaaatt aacaagaacg
    10141 tatgaaataa gggtagtgac attcagttgg ctaaaccttc cactaattaa ttggagggaa
    10201 tagaaatggg aaaataccag gaattagcag aaaacatcgt aaagaatatc ggaggaaaag
    10261 aaaatatccg tagcttgaca cactgtgtga cgcgacttcg tttcaaacta aaggaggaaa
    10321 ataaagcaaa ggatgatgtt ttgaaaaaga tggaagggat agtcaccatc atgaaaagcg
    10381 gcgggcaata tcaagttgta atcgggaatc atgtgccaga tgtctatgca gaagtactga
    10441 cagtagccgg gatttccgat gaagcagaag aagaggtttc ttcaagtagt aatctgttca
    10501 atcgattaat cgacatatta agtggctgtt tccagccttt tttaggtgct ttggctgcag
    10561 cgggaatggt caaaggatta aatgctttgt tcttgtttct caaattttat actgaaggat
    10621 cgggtacgta catcatgctg aatgggatag gggatgcaat tttttatttc atgccagtga
    10681 tcttagggta cacagcagcg aaaaaatttc atctgaatcc gatggtgggg ctcgtgattg
    10741 gtgcagcact ttgttatccg actgtacaag gagcagcact acaaagtggt ttccctgatg
    10801 gcagtcctgc gccttatagc ttgctgggtc ttccggctta tgaaacattt ttgggtatcc
    10861 catgggtggg cagcagttat actagtagcg tcgtacctgt tatcttcatt atcgcatttg
    10921 ccggacaagt ccaaaaaatt gccaagaaat ggattcctgc agtcgtccag acctttttgg
    10981 tttctttttc tgtgttatta atcgcacttc ctgctggctt tttgattatc ggtccgattg
    11041 tcagtatact gacagatcta ttaagcacgg gattccaagc attgatggac ttttctccag
    11101 ctgtgtatgg tgcagttcta gggtttcttt ggcaagtact ggtgattttc ggacttcatt
    11161 ggagtattat ccctcttgcg attatgcagc tagcgcaaaa tgggtggagt cagatgttag
    11221 taggcgttta tggcgcgagt tttgcacaga cagcagtggt attggcgatg tatttcaagt
    11281 tcaaggatca aaaaatgaag gcgctttgca tgccagctgt gatttcagga atcttcggcg
    11341 taacagaacc ggctatttat ggaatcacct taccgaagaa gaagccattt atttactcga
    11401 tgattggtgg tgctgtttca ggaatctatt tgatggcatc tggaacaatc agctacataa
    11461 tgggcggatt agggattttt ggggtggtaa actacatcac ggaaacagac gccagtggta
    11521 tgtttcaagc gtttatcggc attgctattg cttcggtaat cgggttttcg ctgacttttt
    11581 tcttttggaa ggatgaagca ggtgaaacag atgctataca gaaaaaatca ggtaaagaaa
    11641 ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcgaag gatgcagctt
    11701 ttgcacaagg aacactaggt cgaggaatct taatctaccc tgaaaaagga gaagtacgtg
    11761 ccccttttga cggaacagtt atgaccctgt tcccgacgaa acatgcaatc ggaatggttt
    11821 ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga
    11881 aatattttga aagcttagtg cagcaaggag aaagagtgag acaaggcgat ctgttgctac
    11941 gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca
    12001 ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg
    12061 atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat
    12121 tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg
    12181 atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa
    12241 ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc
    12301 gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta
    12361 ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatcattt
    12421 tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga
    12481 atcccgcaca tatcgctcgt acaattgtac gcttcataga atgattcacc aaaagtggtg
    12541 acaattgtga catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa
    12601 gtattcgagt tactggcgaa gatcacgatt ttttttgcct ggagcagtaa atatttagct
    12661 ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa
    12721 gaagccattt tatttgcgat tgtcataata ccgtctgtgg tggaaaaagg aagattcgca
    12781 tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa
    12841 ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga cgggtggaca
    12901 taacatcgct cagctatcgc ttggatggtc atttcgccaa gtgctggaga atcgcttaag
    12961 agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt
    13021 aataacatgt agataccttc ttcttatttt tttacgtaaa ttataacttt tttgaaaatg
    13081 ttttgtaaac ggatttatac tatataactt aaaataggct ttttttgaca agatatttac
    13141 aaaaatccac tagaaaacag taacttttgt aaatgttact ggaaagaaac ggatttctat
    13201 tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa aagtaaagga gtgagaacat
    13261 ggcaaaagga atcaaaatcg ctacgattgg tggaggcagc agttacaccc ctgagttgat
    13321 ggaaggattt atcaaaagat atgaggaatt accgatacga gaaatctggc tcgtagatgt
    13381 agaagaagga aaagaaaagc ttgagatcgt tagtcagatg tctcagcgta tgtgggatgc
    13441 ttctccatat gaagtcaaga tctatgcaac tctggatcgg gaagaagcat tgaaagatgc
    13501 ggattttgtt acgactcagt ttcgtgtcgg tcaattagat gcacgaatca aagacgagcg
    13561 gatccctagt tattatggga tgattgggca agaaacaaat ggtgctggcg ggatattcaa
    13621 agcttttcgt accgttccag tcgttttggc aatcgttgag gacatgaagc gtttatgtcc
    13681 aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg gtgacagaag cagttatcag
    13741 atatggcaaa tgggataaag tgattggatt atgcaatgtg ccagtgatgg cgatgatgac
    13801 agaaccagca atgcttggca agaaaaaaga agagctgatc tacaagtttg ccggtctaaa
    13861 tcatttccat tggcacaaag tagcagataa taaaggaaat gaccgcacgg cagaattgat
    13921 tgatcgttta tacgatgaag ataatggatt accgaaaaat atctttgata tcccgttttt
    13981 caaagaacaa ttaaaacaaa tgaacatgat cccttgtggc taccaccgct attactatcg
    14041 tgaagaagaa atgctggaac atgctttaga agaatatcaa acgattggta caagagcgca
    14101 gcaagtgaaa gaaacagaag cagagctatt tgaattatac aaagatccag aattagacca
    14161 taaacctgaa caattgcaaa aacgaggtgg cgcttattat tcagacgctg cctgtgaaac
    14221 gattgcttca atctatggaa ataaagaaac acagatcgtt gtatctacaa agaatgatgg
    14281 agctgttcct gacctgtcgg cagattgtgt tgtagaagtc actgcctatg ttggcgcaca
    14341 aggagcgaga aatgtagcgt ttggttctct tcctgctgct gaaagaggct ggttgcaagt
    14401 aatgaagaat atggaattgc tgacgatcga agcagctgtg actggtgatt atgggacagc
    14461 tttacaggcg tttacgatca atccacttat acgatcagga aagacagcaa aacggatcat
    14521 ggatgagttg tttattgcac accagcagta tttaccgcaa ttcaaggaaa caatcagccg
    14581 attaaaagaa gaaggaatcg aagttcaaga tgaactggca agagaattat caggtaatac
    14641 acatataatg gaggaagtat aatggggttc agaaaagatt ttttatgggg cggtgctaca
    14701 gcagccaatc aatgtgaggg cgcttatgat gaaggtggac gcggactagc aaacgtagat
    14761 gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg gaaagaaaaa aatgtttgaa
    14821 tttgatgagg atcattttta tccagcaaaa attgcaatcg atatgtatca tcgttataaa
    14881 gaagatattg ctttgtttgc agaaatgggg ttcaagactt atcgaatgtc tatcgcctgg
    14941 agtcgtattt ttccaatggg ggatgagaaa gagccgaatg aagctggctt gaaattttat
    15001 gaggatgtct ttaaagaatg ccaaaagcac ggaattgagc cacttgtgac gattacgcat
    15061 tttgattgtc caatgcattt gatcaaagaa tatggtgcgt ggcgttccag agaactcgtt
    15121 ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgct ataaaggact tgtcaaatat
    15181 tggctgacat tcaatgagat caatatgatc cttcatgcac catttatggg cgcagggctg
    15241 tattttgaag aaggagaaaa tgaagaacaa atcaaatatc aagcggcaca tcatgagttg
    15301 gttgcaagtg ccatcgcaac gaagattgct catgaagtag atccggagaa caaagtaggc
    15361 tgtatgctgg cagcaggagc atattatcca tacacttgca atcctgaaga tgtttgggct
    15421 tctagaaaat cagatcgaga aaattatttc ttcattgatg tccagtcacg aggagaatat
    15481 cccgcttatg cattgaaaga aatggagcga aatggcattc agatcgaaat ggaaccaggg
    15541 gatgccgcat tgctgaaaga acatactgtt gattttatct ctttttctta ttattcttca
    15601 cgagtttcga ctaccgacaa cgaactcttg gaacagactg caggaaatat ctttgcttct
    15661 gtcaaaaatc catatctgca agcaagtgaa tgggggtggc aaattgatcc attaggcttg
    15721 agaataacga tgaatgatat ttatgatcgt tatcaaaaac cattatttat tgtagaaaac
    15781 ggcttaggtg cagtcgatac gccagatgaa aatggttatg tggaagatga ttaccgaatc
    15841 gattacctgg cagcgcatat ccaggcgatg aaggatgcag tagaactaga cggagtcgat
    15901 ctattaggct atacgacttg gggatgtatc gatttagtat cagctggtac cggtgagatg
    15961 aagaaaaggt acggattcat ttatgtcgat cgtgataacc aaggaaatgg cacattagcc
    16021 cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa ttgattcaaa tggagaaaat
    16081 ctttcgaaca attgatcaat gatttataaa agtctattgt ttttcagcgg atttttcgag
    16141 tataataaag ctattcgaga aacgcaagca gaacaataga ctttttttcg cattggctgt
    16201 aaagtatgct tacttgatta agtaaaggag aatctacata tgtatgatgt aatcgttatt
    16261 ggcgcaggtc ctgctgggat gactgctgct ttgtatgctt cacgttcaaa cttatccgta
    16321 ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca atacggcaga aatcgaaaat
    16381 tatcctggat tcgattcgat catgggcccc gatctagctt ataagatgta tgaaagtgtc
    16441 tcaagatttg ggacagaaaa tgcttatgga atcgttcaag atatcaagga tcacggcagc
    16501 cataaagaag tcatctgtga agataaaagt tacgaaggaa aaacagtcat tatcgcaaca
    16561 ggctgtatcc accgtaaatt aggcgtacct ggcgaggaag aatttgctgg acgaggcgtt
    16621 tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca aacgtctagt agtcatcggg
    16681 ggtggggact cagcagtaga agaagccatc tatttgacac aatttgcttc tgaagtagtg
    16741 atcgtccacc gtagagatga attacgtgca caaaagatca tccaagaccg tgcatttgca
    16801 aatgaaaaaa tttcctttat gtgggataca gtcgttgacg aaatcgttgg aaacgaaatg
    16861 gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca atgaatttga tttagcagca
    16921 gacggagtat tcatctatgt tggactggat ccattaactg caccttttag agaagcaggt
    16981 ttgacgaacg aagctggctg gattccaaca gacaacgaaa tgaaaacgaa tatgccaggc
    17041 gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac aaatcactac agctgttggt
    17101 gacggcggta ttgctggaca acaagtgtat aaatacttgg agtctttaga agaaacaaca
    17161 gcataagttt aaaaagagtg aaagaacggt ttcaattgtt cttttactct tttttttcgc
    17221 gtgaaatcca ctgaatccga tttatgtgaa tacttgcaat catgcttgat aaaagaatcc
    17281 tttcttgatt taagcgtttt ttggtcctca tactttcata tctaataagt gaatgctata
    17341 cttattaaga accatatttc agaaagaggt gttattttca tgtcttggga acaagtgtat
    17401 gaacaatggg tacatgaaga aaatttagaa gaaaacttaa aaaaacaatt aaatgatctg
    17461 ggtgaagatc ctgaaaaact tgaagatgct ttttatgcac cgttggaatt cggtactgca
    17521 ggcatgcggg gaatcttagg acctgggatc aatcgaatga acatctatac ggtaagacaa
    17581 gcaacagaag gactagcacg tttcatggat acacaaggtg aagaaacaaa acgccgaggt
    17641 gtcgctatcg cttatgactc acgccatcag tcaccagaat ttgcaatgga agcagccaaa
    17701 acactggcac atcacaatat cccgtcttat gtattcgaaa gtctgcgtcc aacaccagag
    17761 ctttcttttg cagttcgtca tttgaagacg tttgcgggaa tcatggtaac cgcatctcac
    17821 aacccagcgg cttacaatgg atataaagtc tacggagaag atggcggaca aatgcctcct
    17881 gcagacgcag atgcattgac gaaatatgtc cgtgaagtca gcaatccgct aaaagtcgaa
    17941 gtattatctg acgaagaagc taaacatagc gggttgatca cgatcatcgg agaagaagtg
    18001 gacgctgctt atctagaaga aatcaaagca gtgacgattg atcgtgaatt ggttgaaaca
    18061 atggggaaag atttgaaact tgtgtacacg ccacttcatg gaacagggaa aatgttgggc
    18121 gaacgtgcgt tgaaacaagc tggattcgaa caattcgtac tagttcccga acaagctgtg
    18181 gcagatccag atttcactac ggtcaaatcg ccaaatccag aagaacattc agcttttgaa
    18241 tatgctatac gacttggaga aaaagaggga gctgacttgc tgattgcaac agatcctgat
    18301 gcagaccgtt taggcgcagc ggttcgtttg ccagatggtt cttatgaagt attgactggg
    18361 aaccaaatcg gagcattgtt tgctcaatat attttggaag cacatcaatc aagaggcact
    18421 ttaccagaaa acgcagcggt attgaaatca atcgtttcca gcgaattgcc tacagcaatt
    18481 gcggaacact acggtgctac tatgttcaat gtgttgacag ggtttaaatt cattgctgaa
    18541 aaaatccaac aatttgaaga agaccattca catactttta tgtttggatt cgaagaaagt
    18601 tatggttatt tagttaaacc ttttgttcgc gataaagacg cgatccaagc attgctttta
    18661 ttagcagaag tagcagctta ctataaaaaa caaggcaaaa cgttgtatga cggcttgcaa
    18721 acgatttttg aaacctatgg ctatttcgca gaaaaaacga tttctgtgac aatgagcgga
    18781 caagaaggtt ctgcgaaaat ctctgctttg atggaaaaat tccgtaaaga agcaccgaca
    18841 gaatttgccg gagcaaaagt tattcaaaca gaagacttta aagaattgac gagaacagac
    18901 cgtgaaggaa acgtagaaaa actaacaaca ccgccatccg acgtattgaa gtatacattg
    18961 gaagaaaatg gctggatcgc tatccgccct tcaggaactg agccgaaaat caagttttat
    19021 attggtgtaa aaggaagctc tacagaagac gcagccgaaa aagtagcctc ttatgaagcg
    19081 attatcaaaa agatgacaga agaataaaaa acttgagact gatggaaaaa gtggtaaact
    19141 ttagaagtca gtcttttttc aagttggagg tgaatggatt gccagacacg caaaaatcaa
    19201 aagtagagaa agtcagtcaa ttattcaaaa tgttgagtga ccccactcgt cttaaaattc
    19261 tgctatatct gaaagacggg gaacaaaatg tgacagcaat cacccaagca gtggaaatgg
    19321 agcagtctgc tgtatcgcat caactacgtt tattaagaga aaatcatgtc gtcaagtctc
    19381 atcgcgaggg aaaagcgatt ttgtatagtc ttgatgattc tcatgtactg gatattttga
    19441 accagacact caaacatgtg gaacatcaat aacgataggt gc
//
LOCUS       DAYYNQ010000057        19462 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000057 DAYYNQ010000000
VERSION     DAYYNQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.057"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..3644
                     /locus_tag="ACHBJ1_001809"
     CDS             117..3644
                     /locus_tag="ACHBJ1_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM9430744.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3641..7363
                     /gene="addA"
                     /locus_tag="ACHBJ1_001810"
     CDS             3641..7363
                     /gene="addA"
                     /locus_tag="ACHBJ1_001810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM9430745.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7432..7773
                     /locus_tag="ACHBJ1_001811"
     CDS             7432..7773
                     /locus_tag="ACHBJ1_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9430746.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8082..9125
                     /gene="pheS"
                     /locus_tag="ACHBJ1_001812"
     CDS             8082..9125
                     /gene="pheS"
                     /locus_tag="ACHBJ1_001812"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM9430747.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9130..11550
                     /gene="pheT"
                     /locus_tag="ACHBJ1_001813"
     CDS             9130..11550
                     /gene="pheT"
                     /locus_tag="ACHBJ1_001813"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM9430748.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACHBJ1_001814"
     CDS             complement(11720..12730)
                     /gene="trpS"
                     /locus_tag="ACHBJ1_001814"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM9430749.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12984..13721
                     /locus_tag="ACHBJ1_001815"
     CDS             12984..13721
                     /locus_tag="ACHBJ1_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9430750.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13735..14550
                     /locus_tag="ACHBJ1_001816"
     CDS             13735..14550
                     /locus_tag="ACHBJ1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9430751.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14563..15210
                     /locus_tag="ACHBJ1_001817"
     CDS             14563..15210
                     /locus_tag="ACHBJ1_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9430752.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15226..15882
                     /locus_tag="ACHBJ1_001818"
     CDS             15226..15882
                     /locus_tag="ACHBJ1_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9430753.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16044..16865
                     /gene="racE"
                     /locus_tag="ACHBJ1_001819"
     CDS             16044..16865
                     /gene="racE"
                     /locus_tag="ACHBJ1_001819"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM9430754.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16868..18223
                     /gene="rph"
                     /locus_tag="ACHBJ1_001820"
     CDS             16868..18223
                     /gene="rph"
                     /locus_tag="ACHBJ1_001820"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM9430755.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18224..18742
                     /locus_tag="ACHBJ1_001821"
     CDS             18224..18742
                     /locus_tag="ACHBJ1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9430756.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18849..19349
                     /gene="cbpB"
                     /locus_tag="ACHBJ1_001822"
     CDS             18849..19349
                     /gene="cbpB"
                     /locus_tag="ACHBJ1_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM9430757.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6548 a         3342 c         4179 g         5393 t
ORIGIN      
        1 aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag aacaaatgtt
       61 cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg aaaagcatgt
      121 ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg atcgacgaag
      181 catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt cccaactaca
      241 gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc ggaaaaaaaa
      301 ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg tatttgctgc
      361 aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca atgatcttgc
      421 gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa atcaataaaa
      481 gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga aatattcaac
      541 cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag caacttaaaa
      601 tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa cgtgcactaa
      661 caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat ttcaacaacg
      721 tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa ttactgcagg
      781 caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct tatacagcgg
      841 atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc cgtctttttc
      901 aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca gagcagactc
      961 gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc caaaagaaag
     1021 cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct gaaaatccaa
     1081 ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa gaaaactacc
     1141 gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat atccttcggc
     1201 cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg atggaaaatc
     1261 atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct tatcggctaa
     1321 gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca gaaaccgaac
     1381 ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc actgaaaatg
     1441 tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac tggcagtttg
     1501 tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa gaacagtcaa
     1561 atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa ataaagaaaa
     1621 gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa agtggcgtac
     1681 agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta gaagcggcaa
     1741 gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat gtagtaattt
     1801 atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt ggtctagaaa
     1861 atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat cgtttggaat
     1921 tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat caagtgtttc
     1981 cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta ttaaatcagg
     2041 ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct tttgagcctt
     2101 tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt tatgcacaaa
     2161 gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc acagaatatt
     2221 tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct aattgttatg
     2281 tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta gctttagatg
     2341 aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg aattcgtcat
     2401 ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt cctgtttcga
     2461 tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca atttcacgta
     2521 tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg aaattgaaag
     2581 aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat gaatcattgg
     2641 atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg tccgatgcgc
     2701 agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt cggtttgaga
     2761 tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag acgattcaaa
     2821 aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct cttcagacag
     2881 agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa ttaccgttga
     2941 gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta gcttctgaac
     3001 aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca ttcgatgtca
     3061 cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta gcattgatgg
     3121 atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat tatttacatg
     3181 ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg aaaaaattta
     3241 gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat cattcactgg
     3301 aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat gaataccaaa
     3361 aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta ttcatggcgc
     3421 ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa atcaaactaa
     3481 atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc agaagcatct
     3541 gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa atagataagg
     3601 aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa ttcctttaaa
     3661 accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc aaggagataa
     3721 tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc gaagagtgat
     3781 tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat ttactgaagc
     3841 tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg tcaatagcga
     3901 aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc ccacagcgaa
     3961 tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt acttaattga
     4021 tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga tgaaggagga
     4081 tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat ttttccaatt
     4141 aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg tgttttctct
     4201 ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt taagcgataa
     4261 ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa tccgtccttt
     4321 agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc agttagcaca
     4381 aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc agatcaagaa
     4441 tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt tggaacaact
     4501 aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt ccaatgaagt
     4561 caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa gttattttcc
     4621 tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag tcgaagaaat
     4681 gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg aaaaaggtgt
     4741 gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa aaacgaaaga
     4801 tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag aagtaatggt
     4861 agatgaatat caagacgtga accaattaca agaagccatt ttatactggc taagagaacc
     4921 cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca tctattcttt
     4981 ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa agaaagaagg
     5041 cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc tttcttttac
     5101 gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt atgacgaagc
     5161 agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg agccggaaat
     5221 catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg atgatatact
     5281 ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc aattaattga
     5341 ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag agtacaaaga
     5401 cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga ttttcaaaac
     5461 attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga cagaaattcg
     5521 cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc cgcttgcggc
     5581 tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca ttcgattagc
     5641 taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa acgaagatga
     5701 attagcagct aagctggaac attttggcaa gcaactagag cattggcgag aacttgctcg
     5761 ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat atctagagta
     5821 tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat tggttgatcg
     5881 tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc gtttcattga
     5941 aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag aagataatgc
     6001 tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg tatttttgtt
     6061 agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt ttgaagaaaa
     6121 acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg atacgttacc
     6181 gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa tgcgtaaatt
     6241 gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct ataaaaataa
     6301 agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag tttttgatcc
     6361 tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg gattgatccg
     6421 tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc ttcaacattc
     6481 aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac aaagacagtt
     6541 gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg aaactttatt
     6601 agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt cttcacaaac
     6661 cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag atgatacaca
     6721 agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc aatttcgtta
     6781 tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag tgtcagcagc
     6841 tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga tgccaactac
     6901 agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag acgaaaaagt
     6961 cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac ttggtcaaca
     7021 gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc tgcttcctgc
     7081 tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc acggaatcgt
     7141 cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa cagattttat
     7201 tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc aaggacagct
     7261 aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa atgttttcct
     7321 aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat tgagcaaact
     7381 tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat gatggagcta
     7441 acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat tttaggaaaa
     7501 aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg ctttgttgag
     7561 cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt gaaggaatta
     7621 gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg ttcagaatat
     7681 ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca agtttggagc
     7741 gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct tgtaaactat
     7801 agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta aaccagttca
     7861 gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt tcactttccg
     7921 agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt gcggtgtaag
     7981 tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca ggaatgaggt
     8041 ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta caagcacaat
     8101 tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat ttaaatgaat
     8161 taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa gtattacgtg
     8221 ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca aatgagatcc
     8281 gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa gaagcgttaa
     8341 atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa gtaaaacatg
     8401 gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt gtgggtatgg
     8461 gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc gaacggatga
     8521 atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt tcagacgaga
     8581 tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa catgatttct
     8641 ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat acagatgatg
     8701 cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa aacatcacaa
     8761 tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg gaagatcgtg
     8821 aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa gtagatgtca
     8881 gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga tggatcgaaa
     8941 ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc gatccagaag
     9001 aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg cggtatggag
     9061 tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag ttcaaggggg
     9121 agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt atcgaatgta
     9181 acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga gggagtaacg
     9241 gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg cgtaccacat
     9301 ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga actgtctcaa
     9361 atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc acttcctaac
     9421 tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca agtatcaaat
     9481 ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc gaaagaatac
     9541 gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt ttttccttat
     9601 ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc ggatgcattg
     9661 tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc tacattcgat
     9721 gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt ggaagttgaa
     9781 gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag aatccagcca
     9841 agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat caacaatgta
     9901 gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc atttgactac
     9961 gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga aacattagta
    10021 acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac taacggcaaa
    10081 gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac gaatgaaaca
    10141 acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg tacagcaaaa
    10201 caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa ccatgcgact
    10261 gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg tggggaagtt
    10321 ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt gaagatttca
    10381 cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt aaatgagatt
    10441 tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt agtagtacca
    10501 ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc tcgtatttat
    10561 ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg aagcttgtca
    10621 aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg attgagcgaa
    10681 gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat aaatgaaagt
    10741 aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg aatgaatttg
    10801 atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac agagattgct
    10861 ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca tctgccgcaa
    10921 gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga ttggcagacg
    10981 aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt atttgaacaa
    11041 ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga aatgcatcct
    11101 ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg tcaagtgcat
    11161 ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt tgatttatct
    11221 gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa attcccagca
    11281 gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga attaacagaa
    11341 gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga tgtctatcaa
    11401 ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt cgtcaaccca
    11461 gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga aaaagcgttg
    11521 gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta acgatcaagt
    11581 agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca catctctttt
    11641 tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct ttacagcctg
    11701 cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat tttacttcag
    11761 aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag atttccataa
    11821 cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca aattcagctt
    11881 ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg cggcggtaat
    11941 gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag taggtgaaga
    12001 ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga tcagtataca
    12061 tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata ccgtttccta
    12121 acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt ccttttggtg
    12181 ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag ctttgtacga
    12241 tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca agattcgctt
    12301 tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct gtaggcacgc
    12361 tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc cgaacgcgtc
    12421 ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga atttgtgatt
    12481 ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct aaagcaactt
    12541 gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt gcttgcatgt
    12601 cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact cgggttttta
    12661 gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc gttaaaataa
    12721 tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa tatcttaccg
    12781 tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg ataaaataac
    12841 taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag aaactttaca
    12901 aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga aatcttacta
    12961 aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg gataaatatt
    13021 atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga gaagttgtcg
    13081 ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc aatggattag
    13141 agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat aaaaagacaa
    13201 aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat ttgtacccaa
    13261 ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag caagaccagg
    13321 caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta gataagaaag
    13381 attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc gccagaggtt
    13441 tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg gatccggaaa
    13501 tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg tcaatgattg
    13561 tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc ttcatggctg
    13621 atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat ccaaatgaag
    13681 tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa aaagatgcgc
    13741 aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc actagccgga
    13801 tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc taccaatgaa
    13861 atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat tgagagcaga
    13921 actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat ccttggtgac
    13981 aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt attgaaaaac
    14041 ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa aaaacaggtt
    14101 gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa aggaagccaa
    14161 atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa aggttctaca
    14221 tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt agaaaactat
    14281 gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac ggataatgcg
    14341 attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg aacgtttact
    14401 aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa agctgttaac
    14461 caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca aaaatggttt
    14521 ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga attgatacaa
    14581 acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag tgtcttagca
    14641 cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc aaagaataaa
    14701 gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc attattaatc
    14761 atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga tggattccaa
    14821 gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac agtacgttca
    14881 gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg ttttacctat
    14941 tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat cccgccatta
    15001 ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt agctggttta
    15061 gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt cgatacgtat
    15121 atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt aatggtttat
    15181 cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga ttttagcggc
    15241 gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac tgttgaagtt
    15301 tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt gattcgtttt
    15361 gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat acggaatctt
    15421 ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg cattcagatg
    15481 aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat gttgtcggaa
    15541 attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg attatcaacg
    15601 ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt taaatcaatg
    15661 atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt agcagtaatc
    15721 atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa caccaattat
    15781 gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta cgcgctttct
    15841 ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat tcttttagcg
    15901 gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa aagagatttc
    15961 gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca caatagggag
    16021 gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct atcggattta
    16081 ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa ttaccgaatg
    16141 aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga cccgcggaac
    16201 aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga atcaagatgc
    16261 tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa gctgctcttt
    16321 ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa aaaacacaaa
    16381 ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct tatgaaaaag
    16441 cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc aaatttgttt
    16501 cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg gcagaaacat
    16561 tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc cattatccat
    16621 tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc gattctggag
    16681 cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc aattcaccgc
    16741 aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt ttcgaggaaa
    16801 tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa ttgggaggaa
    16861 aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg agcttcaaac
    16921 aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata caacagttat
    16981 ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag gaacaggatg
    17041 ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc gtagagaaag
    17101 cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg gtcgttcctt
    17161 gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag actgcgatgt
    17221 gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg tggcattgaa
    17281 attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa taaaagaaca
    17341 tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag atttggatta
    17401 ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat ctggaaaatt
    17461 cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt tgaatgaaat
    17521 gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac atgctttgct
    17581 aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca tcattgcgac
    17641 aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg gatatcatgt
    17701 aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca gcacatttga
    17761 agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc cagtcttagc
    17821 agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct attctgcacg
    17881 ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat atgaattgac
    17941 agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat tcgcggcacc
    18001 caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc gtataccatc
    18061 tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga aacaaacagc
    18121 agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag caatgaaaaa
    18181 attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt atttagtagt
    18241 aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt atcgttctga
    18301 agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc cattatggga
    18361 tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac agaaaaaagt
    18421 gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga atgtccgatt
    18481 tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg tgttttttgg
    18541 tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga atcctggtag
    18601 catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg aaagtttaga
    18661 tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag agttggcctt
    18721 ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa taagctttat
    18781 tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat agaaatggag
    18841 gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag gaaacgtttt
    18901 tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc catggtttac
    18961 ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat gatcactttg
    19021 taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa acaatcagca
    19081 tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt ccgacaattg
    19141 atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct tttttaccgg
    19201 ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg cttaaagccg
    19261 taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa atcgaagaat
    19321 taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata atcaaaaaat
    19381 aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct gggacaaaag
    19441 cgtttaactc caagaaataa ga
//
LOCUS       DAYYNQ010000058        18219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000058 DAYYNQ010000000
VERSION     DAYYNQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.058"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1079
                     /gene="bsh"
                     /locus_tag="ACHBJ1_001823"
     CDS             105..1079
                     /gene="bsh"
                     /locus_tag="ACHBJ1_001823"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9430758.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            1404..3299
                     /gene="asnB"
                     /locus_tag="ACHBJ1_001824"
     CDS             1404..3299
                     /gene="asnB"
                     /locus_tag="ACHBJ1_001824"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM9430759.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(3494..3940)
                     /locus_tag="ACHBJ1_001825"
     CDS             complement(3494..3940)
                     /locus_tag="ACHBJ1_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM9430760.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            4168..4317
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_001826"
     CDS             4168..4317
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9430761.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            4405..4923
                     /locus_tag="ACHBJ1_001827"
     CDS             4405..4923
                     /locus_tag="ACHBJ1_001827"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM9430762.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(5000..5956)
                     /locus_tag="ACHBJ1_001828"
     CDS             complement(5000..5956)
                     /locus_tag="ACHBJ1_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430763.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            6122..6928
                     /locus_tag="ACHBJ1_001829"
     CDS             6122..6928
                     /locus_tag="ACHBJ1_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430764.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            6925..7914
                     /locus_tag="ACHBJ1_001830"
     CDS             6925..7914
                     /locus_tag="ACHBJ1_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9430765.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            7911..8915
                     /locus_tag="ACHBJ1_001831"
     CDS             7911..8915
                     /locus_tag="ACHBJ1_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM9430766.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            9047..9589
                     /locus_tag="ACHBJ1_001832"
     CDS             9047..9589
                     /locus_tag="ACHBJ1_001832"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM9430767.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            9609..10307
                     /locus_tag="ACHBJ1_001833"
     CDS             9609..10307
                     /locus_tag="ACHBJ1_001833"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM9430768.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            10328..10540
                     /locus_tag="ACHBJ1_001834"
     CDS             10328..10540
                     /locus_tag="ACHBJ1_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM9430769.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            10540..11502
                     /locus_tag="ACHBJ1_001835"
     CDS             10540..11502
                     /locus_tag="ACHBJ1_001835"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM9430770.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            11520..11915
                     /locus_tag="ACHBJ1_001836"
     CDS             11520..11915
                     /locus_tag="ACHBJ1_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM9430771.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            12078..12443
                     /locus_tag="ACHBJ1_001837"
     CDS             12078..12443
                     /locus_tag="ACHBJ1_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM9430772.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            12440..14251
                     /locus_tag="ACHBJ1_001838"
     CDS             12440..14251
                     /locus_tag="ACHBJ1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM9430773.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(14313..14480)
                     /locus_tag="ACHBJ1_001839"
     CDS             complement(14313..14480)
                     /locus_tag="ACHBJ1_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM9430774.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            14729..15718
                     /gene="galE"
                     /locus_tag="ACHBJ1_001840"
     CDS             14729..15718
                     /gene="galE"
                     /locus_tag="ACHBJ1_001840"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM9430775.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            15730..17214
                     /gene="galT"
                     /locus_tag="ACHBJ1_001841"
     CDS             15730..17214
                     /gene="galT"
                     /locus_tag="ACHBJ1_001841"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM9430776.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            17238..18218
                     /locus_tag="ACHBJ1_001842"
     CDS             17238..18218
                     /locus_tag="ACHBJ1_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9430777.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         5747 a         3116 c         3840 g         5516 t
ORIGIN      
        1 tataaatgct agtaaatcaa tcttctttgg cctttttttt gataaaaaaa aatactagat
       61 gtaagataaa agtgtaaaaa cgatttgttt taggagggaa aacgatgtgt accgcaatca
      121 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
      181 aagtagtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
      241 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
      301 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
      361 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
      421 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
      481 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
      541 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
      601 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
      661 cgctatcaaa tggaacacca gaaaatcatt tttcaaacca gatcagcttg aatgtttata
      721 gtcgtgggat gggaggattg ggcttacctg gagatctctc ttctgtttct cgatttgtaa
      781 aagccacatt tactaaaatg aatgctgcat caggtgattc agaatctgaa agtatcagtc
      841 aattttttca tatactaggc tcagtcgagc aacaaaaagg agtatgcgat actggagaag
      901 gaaaatacga atatacgatt tattcttctt gctgtaacgt tgataaaggg atttattatt
      961 atcgtacgta tgaagacagt caaattactg ctgtaaatat aaataaagaa gacttggatc
     1021 gtcatgaatt ggtcagttat ccagtcataa aagagcaaca gataaactat gtcaattaat
     1081 taatgaatag atccgtttgg tatcatattt tctgatacag tcataattag acacgatctt
     1141 taggtcgtgt ctttttttgc tgtttttttt ggaaatagca tccactaata tttttttaac
     1201 acgaagagaa taaggtagcc tcaaaaaaga aaacatggta aattagatat tgggacttta
     1261 ttttctgtaa aaacagaaac tttcatcttt tgttttcacg tgacgaaatt gtaagagaga
     1321 aaatattcgt ccgctattta ttctatgata agatgcatat gaatgaaata aacagtaatt
     1381 ttatattagg atgtgaaagt taaatgtgcg gtattgtagg ttttgttaac gacaaggaca
     1441 acaaaaaaac aattattaat acaatgatgg atcgaatcgt tcaccgtgga cctaatagtt
     1501 caggagaata tatcgataaa catgttgctt taggattcag aagattaagt attattgact
     1561 tagagggcgg tactcagcct atttacaatg aagatcggac aaagatcatt atttttaatg
     1621 gcgaaatcta taattaccag cctttgagag aagaattgat tgcagctggc cacgtttttc
     1681 aaacacatgc agatacagaa gtccttttgc atggttacga agaatgggga acagaattat
     1741 tgcaaaagat ccgcggaatg tttgcttttg ctatttggga taatgaaaag aatgagttat
     1801 tcggtgcaag agaccacttt gggatcaaac catattatta tgcagaaatg aacggaacat
     1861 ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc tgattttaac aaagaattga
     1921 acaaagaagc gttgaaacct tatatgactt tccaatattc tccattaaac ggcgaaacat
     1981 tcttcaaaga tgtctatcgt ttgcctgaag gccattatta tacgtataaa gatggaaaac
     2041 tagacattca gcaatattgg gatgcagatt ttgaaacaaa agaaacgcat tcccgacaag
     2101 aatggatcga aaaaatcgac gaaaccgttc aagcttctat tgaagcacat acggtcagtg
     2161 atgtagaagt tggttctttc ttatccagcg gggttgattc cagttatgta acctctgtat
     2221 tgaaaccaga tcattctttc tcaatcgggt ttgatgataa aacctataat gaagcgattg
     2281 aagccagaaa actaaccgaa ctattggact tagataatac agcagcagtg atcgacggag
     2341 acatgtcgtt caaagcattc cctttgatcc agtatcactt agatgaacct gattcaaatc
     2401 cttcttgcgt acctttgtac tttttagcaa atcttgcttc acaaagtgtg cgggtagtcc
     2461 aatctggaga aggggcggat gaactgtttg cgggatacca aacgtatgga ttccatacga
     2521 attcaaaatt catccgtgtg attgctcaag gattgaaaaa attaccaaaa ggaacacgct
     2581 ataatttagg acgtaaaatc ggaaaaatga aaaatttcca tggacggatc catttatatg
     2641 aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc tcgagtgttc gaagaatcag
     2701 aagcatcaga agtgttaaca ccaaaatatc aaacagcacc atctgtggat gaaatcatga
     2761 ctgttcatta cgagaaaaca gaaggaatca aagatgaagt caataagatg caatatgtcg
     2821 atcttcatca atggatgcca aaagatatct tgttaaaggc agataaactt tctatggcaa
     2881 gttctttgga agtccgagta cctttattgg atatcgaagt catgaaattg gctcaacaaa
     2941 taccaagtaa gtttttattg aatcaaaaca acacaaaaga tattttcaga caagctgcga
     3001 acaaacatct tccggaagaa tggtcgaatc gtgtgaaatt aggattccct gtgccaatca
     3061 aagcttggtt gaaagaagaa cacggctacg aacaagtcaa agcattattc gaagcagatt
     3121 ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt gctagatgac catcacgaag
     3181 gacgtaaaga agagcaaaga aaaatctgga caatcttcag tttcttgaca tggtataaag
     3241 tctactttgt agatgaaacg attccgcaag cagaagcaat cgactatgtg actgtttgat
     3301 gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg tttaacactg agtgataaga
     3361 aacaatttca aaaaacagct cttcccgttt tatagatatt atgacttatg gttcggagtt
     3421 ggcagaggaa caccattgtt tcgaacataa gggggttgtg acacgatttt cgtcacaacc
     3481 cctttacata tgttcaataa tcgatttctt tttcttttgt cccatctttt ttataaatga
     3541 acaaagttga ttctttgttt tgtgctactt gctttccttt ttcgattgct tctttttttg
     3601 tatcaaaatg gttgcttgcc ttttttgccc ctttagattt cacgatccat tgttcttcct
     3661 catattctac gatcacatct gaatcaagca gttttccagc acgaggattc gtatcgtgtt
     3721 catccgtctt gcttggattt ttttcttttt cgaaagttcg tttttctgat tcactagcat
     3781 cttcatacca tttttttgct tgatcaatgg caattgggat cgcacggtta tcagggtacc
     3841 cttcatctaa taatgcattt gcgatgtcga ttgctttttt tcttgtcaat ttatctaagt
     3901 ttttcattga tgcaggaaag tccttcatgt tccatggcat attagtatcc ccctttatta
     3961 aaaaacataa tctatataaa caagtgtaag ggtatttata caaaaagaaa aagattttgt
     4021 ttgagaagta aaaccgttgt atttatcgtc aagtagtttt acttaacaaa atcgtgaaaa
     4081 acactagcaa tctagaaaaa atcatggtaa gataatcaag tctgagagtc atcttagagg
     4141 attcttgtaa aaatgggagg gaaaaacatg cgcgtaaaca tcactttaga gtgtacttct
     4201 tgtaaagaac gtaactatct aacaagcaaa aacaaacgta acaatcctga tcgtttggaa
     4261 aaacaaaaat attgcccacg cgaacgcaaa gttactttgc accgtgaaac aaaataatgt
     4321 ttttaaagtt ctcaattcct tgctggtcaa agaattgaga ctttttttat ctcaaaacat
     4381 ctaaaatcat aaggagcgga taatatgttt atttccatgt gggcacaaga taaaaatggt
     4441 ctgatcggca aaaatggttt gttaccatgg cgattgccaa atgatatgcg tttctttcgc
     4501 gaacatacaa tggatcgaat tttagtgatg ggaagaaaga cctatgaagg aatgggggat
     4561 ctttcacttc cttatcgcca tatcatcgtg ttgacaacgc aaccaaattt tcgcacgaaa
     4621 gaaaatgcgg aagtaatgca ttcgatcgat gaacttttgt cgtatagtca aacgatctca
     4681 gaagatatct atgtttcggg tggtagtcgg atttttcaag aacttcttcc ttacactggt
     4741 cggatctggc gaacattgat cgatagcacg tttgaaggcg atacgtatat tgggaatatt
     4801 gatttttcag acttcgccct tatagaggag tatgagggga taacggatca agaaaatcta
     4861 tatgcccatc gttttcaaaa atgggaaaga gtaaaaaata gggttgcttc aaaagaagaa
     4921 taaaatctat gcgtctagtt tgttaataag aaaaaaagca tctcacgact aatcgtgaag
     4981 attctttttt tagttaaaat caatttagtt tttctttgat atcattgacc atttcctgac
     5041 tggcaatagg tccgttatat agccaactag attcaggtcc gaattgcagt acatgattgt
     5101 ctgtcactgc tttaagattc ttccagcttg gttcattgaa catggcagca tctttgtcac
     5161 tgttcacaag gatgatgtag tcagcatcta attcagctag cttttcagag gaaacagcag
     5221 accagtcaga agtggctttt ttggagacct cttccactag atcaggaaca ccaaattcca
     5281 gatcgtcgta tacgattctt ccacttgacc gatttttgct gaccataaag gctgagttgt
     5341 tggtcaccca caacactgca gctgtcttgt tctcgatttt atctgctagt tcttctcgag
     5401 ttttcttgat ggatttttga taatcggaaa tgattttttc tgcctctttt tcttttgcca
     5461 aagctttgcc gacatctttt agttgttctt cccatgtgac atctgttccg ttttttacaa
     5521 cataagttgg cgcgattttg ctatattgtt cgtatttccc gccttcgaca gtagcggaag
     5581 aagaaatcaa cagcaaatca ggctcaaagc ttaacacttt ctcataagga aggtcataag
     5641 aaatcgttgg gacatccttt aaatcatctt gcagataatc ttgaatggaa ccactgccaa
     5701 cagtccactg ggcgataggt ttttccccta gtgcaatcag atagtcttct aagtaactgc
     5761 cgatgatccg tttgggcgcg tttggaatct ctactttatg tccaagtgta tctgtgactg
     5821 tatgagtact attggatgaa gtcgtcgaat tggcagttgt agctgtttct ttatctgaag
     5881 agggggagca agcgctcagt actccagcca gcagcaatat gctagataat gtgaatactt
     5941 ttgtttttaa tttcatttct ttcctcctaa cataattgag aatctttctc attggttatt
     6001 attcctgata tttcgaacgc tgtcaaccgc ttcatttggc tatagatatg aaagtagcgc
     6061 ttgacgaaat gttgaaacag cgtcataatt gagaatgatt ttcagcgaaa ggatgggtga
     6121 aatgacgaga ttaagagcag ctgacattgc agccggctac gaaaacaaaa aaatcattga
     6181 aaacatgtct gttacgattc ctgaagggaa aatcactagt ttgattgggc caaatggaag
     6241 tggaaaatct actttattga aaagttttac ccgtatacta cctgtgaaat caggaaagat
     6301 cattgtcaat aactctgata tctctacata cagttcaaaa gaacttgcaa aaaaaattac
     6361 attattagca caaactagtg aacaccctac aggtatgaca gtagaagaag tagtatctta
     6421 cggacgatac ccttatcaaa aactgttcag tggcatggac acgcaagatc atgaagcaat
     6481 cgagtgggcg ttggatgcta caggtctagc tggatttcgt atgcgggata tcgcaacgct
     6541 gtctggtgga caaacccagc gtgtctggat cgctatggcg cttgctcagg aaacagatat
     6601 cttgatcttg gatgaaccga caacattttt agatccggca caccaattag agatcctaca
     6661 gttattgact gaaatcaatc aaatgaaaca aacaacgatc ttgatgtcga ttcatgacat
     6721 taatcatgct tcacgttttt cagattattt attagcaatg aaaaacggta agttgatcac
     6781 agaaggtaca cctgaagaag tgatcacaaa agagtgtatg aaaaaaatat ttgagattga
     6841 tgcaacgatt gtcacgcttc caagaacaaa taaaccagtt tttctaacct atgatttgtt
     6901 gccaaaagag caggaggatc aattatgagg actatgaaga aaaatagact cctgcttttg
     6961 gtctgttttg ttcttttgat tatttttttt gttgctcttt caaatggcgc agcgactgtg
     7021 ggaaaagcag atttttggca ggcgttgttt gattttgata gatcaaaaca aagtcaccaa
     7081 atcatacgaa atattcgttt gcctcgtgtg gcgggagcat ttcttgtagg gagttgtttt
     7141 gcattgagcg gtgcattgat gcaaggagtg acaagaaatc ctttagcaga ttcaggctta
     7201 ctaggaatca acgcgggtgc ttcgttggca atggccctta gttttgcttt ttggcctaaa
     7261 gcttctgatt tttctgtgtt tctaatgtca ttggcaggtt cttttgctgt aactttgctc
     7321 gtatttggtt ttttgcagtt ttcgaaaatg ggcatgaatc ctttacagct gattttagct
     7381 ggcgtagcta ttagttcttt ttttactgct atcagccaag cacttaccca attctttaat
     7441 ctgcagcagg atcttgcttt ttggtttgta ggcggagctg ccaatatcac atggaaacaa
     7501 ttagcagtat tgtttccttt atatatactt gctgttttgg tttctttttc attaggaaaa
     7561 gcaatatctt tgattcattt gagtgaaaca catgcattag ctcttggaag tcatcctaaa
     7621 cgaacgcggt tgattgtttt ctgtctagtt gcagtgttag cagccattgc cgtttcgtta
     7681 gtcggacctg tttcatttat tgggctgttg gttccgccag ttgttcgcgg aatggttgga
     7741 acggactatc gttatgttct acctgtctct tttttagttg gcggtggatt ggttatgttg
     7801 gcggatttta cagcgaggat gatcaatccc ccttttgaaa caccttttgg attagtcatt
     7861 gcattgattg gcgttccgtt tttactgata caagtaagga gggaacaacg gtgaaaaaaa
     7921 tatattggac gttctatttc ttgctttttg tacttgtcat cctgtctata ggtagtttaa
     7981 tgattggtac gccttttatt tacctggatc agctttttgc cacgttcata gggaaagaat
     8041 catcttcttt ggcgctgatt gttagagaat ttcgagttcc tcgattactg atcagtttat
     8101 tagcaggagc ttgtcttggc gtttctggtt atatcttgca aggagtaaca agaaatgaat
     8161 tggcagattc tagtatattg ggcattaatg ccggagcagg atttttagtg atgctttatt
     8221 taggtttctt ttcgcactat tcgctgccgt ttctattgct agttgtagcc tttctaggtg
     8281 gtgttttagc tgcttgctgt gtctatcttg cggcctatga tcgaaatggc tttttgggta
     8341 tgaatcgaat cttattgtcc ggtattgccg ttaatgccgg cctttctgca ttaacattgc
     8401 tatgcacgat ccaattatct aaggaaaatt atggatttgt gaatgcatgg ttagcgggat
     8461 caatctgggg tgcgagctgg ccttatgttt gggcattgct tccatgggcg gttatcttaa
     8521 ttccgctagg aggactttat gcacagagga ttggattgct atcatttggt caagaacgcg
     8581 cgcaaggatt aggccttaac gtggctaaaa gtcaaaaaat ccttttgttg ttagctgttg
     8641 ctttggcaag cggtagtgta gcggtgacag gtagtttatc ttttgtagga ctactagctc
     8701 ctcatgctgc gaagctagtc gtacgtaaag aaaataactg gacctttgtt ctgagcggtt
     8761 tgttcggttc catttttctg cttagtgcag atatggttgc tagagtgatt ctaccaagtg
     8821 gggaagtacc tacaggaatt ttgattgccc tttttggtgc gccatatttt ctgtatgcac
     8881 tttttatcag acaaaaacac gcgctgaaca gttgaaattt attaaaaaag gaaggccaat
     8941 catttctagg tcttcttttt attctattta tgcatttatt tgaaagttcg attgctattt
     9001 ttcttttttt cctataccat tttatgagag aaggaagggt gagtttttgg accaattgaa
     9061 agtaagatta cgccggaaag gaatcgaaaa gctgaaaaat ctggccaaaa gtggcaagaa
     9121 aaaggaaaaa gaacagcgaa tctataaaca gctattcgcc agcgagtact ggaagtcagc
     9181 aaaagtgata gggacgactt tatcgaatga atttgaactg gatacacagc cggttatcca
     9241 acgagcagaa aaagaacaaa agaaaatagt tgtacctaaa actttgccga aacgtcagat
     9301 ggcgttttat gaaatggatg aacagtccgt tcttgtacgt tcgagttttg gtgttcttga
     9361 gccacaatca gagaagctct atttgccgga gatgatcgat ctcctcattg taccaggtat
     9421 tatctacaat agagccggtt atcgaatcgg ttttggcggt ggttattacg accggtattt
     9481 aaataatttt cataaaaaaa catgtagcct tgtttttcgt gaacaattga atgaacaatg
     9541 gcaaccggag agctttgatc agaagatcca acaattgttt attgactaaa atgaaatggg
     9601 aggttttcat gaattatcaa cagcaaataa aattgcgtgc atggttaaga cgaccgttat
     9661 ggacctatgc ttttttaggg atacagacag cggtcttcct catgatggaa ttatttccgc
     9721 gacttgagat cccttattat gccggaatgt atggtcctta tcttgttcac ttcaatgaat
     9781 ggtggcgttt agtgacacca atcttcatcc attttggact gatgcatttc gtaatgaact
     9841 cactgatact gtattttatg gggcagcaaa tcgaggcaat ctatggccat tggcgttttt
     9901 tcttgattta tatgttcagc gggataatgg gaaatacagc aagctttgct tttaacgaag
     9961 cgaatgttct gtctggtggt gcgagtactt cgatattcgg tttgttcggt gcgttgttta
    10021 tcttagggtt tcattttaaa cataacccag cgatccagca gctagtcaga cattttcttt
    10081 tatttattgt gttgacattt gtttttggtc ttctggatac ctctgtagat gtttggggac
    10141 atgtcggtgg gctaattggc ggattagtgt tagggaatgt tttgggtcta ccgaaacaac
    10201 agacaagcta ttcgatacac caaaggattc tttctacact agtttttgta tttctttttg
    10261 taatatgtat tttactgggt ttaaaaaaat atggtttact tgtataatat gaaggaagtg
    10321 cttaaagatg gagagcttat atgatgtaca gcagctgttc aaaaaatttg gcatctttat
    10381 ctatgtgggt gcacgcatct atgacattga attaatgact atcgaattgc gtaatctgta
    10441 tgatagccgt ttgattgatc atgatacata catgacagcg tggcgcatat taaaaagaga
    10501 gcaccgaatt gaagaaagcc gaaagaaagg aaatttgtaa tggacaagaa gattattggg
    10561 attgatttag gtggaacaac agcaaaattt gcaattttaa caccagaagg agagattcaa
    10621 caaaaatgga gcatcgatac caatattttg gatgatggta agcatatcgt tcctgaaatc
    10681 attgaatcaa tcaaccatcg tttgaattta tataatatga aagccgaaga ctttatcgga
    10741 atcggcatgg gtaccccagg aagcgtggac agcgaagctg gaacggttat tggtgcgtat
    10801 aacttaaact ggacagaagt tcaatttgtg aaaaaactga tcgaggcagg aacaggaatc
    10861 aaattcgcta tcgataatga tgcgaatgtt gctgctttag gtgaacgatg gaaaggtgct
    10921 ggagaaaacg atccagacgt tgtattcgtt actctaggaa caggtgttgg cggtggtatc
    10981 gttgctggcg gaaatttgat tcacggcgtg agcggagcag gcggagagat cggccatatc
    11041 actgtggatc ctgaagggtt cgaatgtact tgcggtaaac gcggatgttt agaaactgtg
    11101 tctagcgcta ctggtgtggt acgtctaggt cgttacttgg cagaagaata cgcaggagat
    11161 tcaaaactga aagcaatgtt agacaacggt gaagaagtaa caagtaaaga tatctttgaa
    11221 atggctcaag aagacgatcc gtttgcattg atggttgttg atcgcgtatg cttctactta
    11281 ggtttagctt gcgccaactt ggggaataca ttgaatccgt caagtattgt gttaggtgga
    11341 ggagtatcgg cagcaggaga atttttacgt agccgtgtag agaaatattt taaagaattt
    11401 actttcccgc aagtaagaga atcaacaaaa atcaaattag cagagcttgg taatgaagct
    11461 ggtgtcattg gtgctgcctc attagccttg aaatttgctt aaaaaaacgg aggcattaca
    11521 tggttttatg ggtaattaat atcattttgc tattgattgt gcttgcaatc atcttttggg
    11581 aagtctatct acgagtaatg gccaagcgtt cagcaacgac tttgacagaa gaagaatttc
    11641 gagaaggaat gagaaaagca caagtaatcg atgttcgcga aaaagatgtg tttgatgctg
    11701 ggcatatttt aggtgcaaga aacatccctt acactgtttt gaaagattca ttaggatcta
    11761 ttcgaaaaga ccagcctgtc tatatttacg atcaaaagaa aagcttgagc atccgtacag
    11821 caaataaatt acgtaaagca ggttatcaag agatttattt acttaagggt ggatatgacg
    11881 gatggtctgg aaaaatcaaa agtaaaaaag tataaaacag atgattgtat gacagcttta
    11941 aaaaccgaat caatcattag attttgacca agatcacatg aacggcgcaa tggatagttg
    12001 tgccgttcat tttttttatt ttgtgctatt atctacatat ataagctaag cgaaaaggca
    12061 gaaagaagga aacaactatg atcaaattaa aacgtgttta ccttgatgct gaaaaagagg
    12121 atggctatag aatactcgtg gataggttct ggccacgagg aatttcaaaa gaaaaagcgc
    12181 agttagatct gtggttgaaa gaaattgcac ctagtaatga agcacgcaaa acttttgacc
    12241 atgtaccaga acgatttgct tcatttgcaa agaaatacca gcaagaattg attttcgaag
    12301 aagaaaagaa gcaagctatt tttaaactca gtcaacttat gaaagaacat ccagtcatta
    12361 cttttgttta tggtgcaaaa aatgaacatc agaaccatgc tgttttattg aaaaaattta
    12421 tcgaaatgga ggcatgcaaa tgaaaatagg gatcattggt gccggaccta gaggattaag
    12481 tatagcagaa aggattctcc ataataatca ggacagacat tctcttgaac tttttctttt
    12541 cgatcccaat ggtcctggag gtcgagtttg gcggttgaat cagccaccgg agttattaat
    12601 gaattctgtt tctcaacaag tgacgctttt tacagatgaa acattgtcca gtggaggatt
    12661 ggtaacacag ggacccaact tgtttcaatg gagtaaagga cctgctacag attatatcga
    12721 acagacggta accaaaaata agcaactaat tatagaagaa atcaatcgtc tggaaaaaga
    12781 tcagcaatct actcgatgtt tatacggact ctatcaacgc tggtttttct caaagttgca
    12841 agaagaattt ccagcttcta ttcagtttgt ttattcttta gtaaaaaaag tcagcaaaag
    12901 aaaagatggt tatcttttac agacagatca gcaaagctat tctatcgatc agcttgtttt
    12961 agcaagcggt cactgggaaa atgatgagac agaagaagaa agagtgtggg tagactacgc
    13021 aaagaaaaat gatttactgt atcagcttcc ttctaatcct gcagatgtcg atctttcgaa
    13081 gataccgcct aaagaaaatg tctttttaag aggtctaggc ttaagttttt ttgattatgt
    13141 cggattgttt acagtatcaa gagaagggcg cttcgagaac gaaaaaggaa aacttgtcta
    13201 tcatccatca ggcaaggaac cgattgtata cagtggatca agaagggggc tcccttatta
    13261 ccctcgagga agaaaccaaa agcaaggcgg atcaatggca tggccacggt tactaacaaa
    13321 agaaaacctg gagcaatggc atcagtcggg atttttgact ggcgaaacat tttttgacta
    13381 tctgaaaaaa gacgttgagt tgttttatta caagaaaatc atcgaagaat atcatttgcc
    13441 gattccaaag aaaaattttg agcatgattt tctctccctc tcttcagagg agtgtctcgg
    13501 aaagcatcca gcactaatcc cttatagatg ggattggtcg tatttagaaa ttcctcttac
    13561 ctatcaggat gagtcctttg cagaagctag ccgagcattt attgaagggc agataaaaga
    13621 agcggaaaaa ggcaattgca caggagcatt tgcttcaaca tttgatgcat taaaagactg
    13681 gcgtgatcca atccggcagg ctattgaatg gcacgttttt tctgcaaagg aatacaaaga
    13741 aatactatgg ggatggttta ctcatctaaa tagcttttta acgatcggtc cgccagtcat
    13801 tcggacgaaa gaattagcag cactgataga tgcgggtatt tttcatttgt tcgaaccgcc
    13861 atttgatatt cagcaaaagc acaaccgcta tgttatttct ctacaagata aagaaatcag
    13921 cagtcgctat ttgattgaag ctcgattgcc acaaacaagc ttttctcgtt caagtaatcc
    13981 tgtcataaaa aatttgaagg aaaacaaact tgttcgccct ttttcatttt cagataatac
    14041 aggaagttat cattcagaag ctatagatgt tgagcgaggt acgaatcgcg tgattgatgc
    14101 ttcaggggaa atccagtctg acttttattg ctatggaata cctgttgaag gtgtggattg
    14161 gctaactgca gcagtagcta gaccttatac agatccatgg aatctgagac aggcagataa
    14221 aatagcacaa cttatattga ctgacaaata aaaatacgca tgatgactta tctgttcttt
    14281 cacaagtctt catgcgtgtt tttaaataat tattaacgag aaacgcgttt gcgcattgct
    14341 tttttacggt tttcttcgat cattgcttct ttttcgtcaa ttggatcgat cactgttttt
    14401 ttcacagaag cgaccaaacc agcaacggca gcggctgttg ccagacttcc tactaatatt
    14461 ccagatacaa attttttcat tgaatccacc tcatttatta tttgttatat ctattatgga
    14521 acaaaagaga aaaaaaggct agttttacgt ttgtttgtaa acctttgcaa aaaatccttg
    14581 aaaatgttta ctaaatgttt tattataaat ttaccatatg aatttacgaa attttacttt
    14641 gtttgttttc accataaaag taaacttcgt ttttttaaat tatatataat caacaaacac
    14701 aattttacta atcaaaggag tgttttttat gacaatttta gtactaggcg gagccggtta
    14761 tatcggttcg cacgctgtag accagctagt tcaaaagggg tatcaagtag ctgtggtaga
    14821 taatttgctc acaggtcaca aacaagcagt acatccagat gctcattttt atgaaggaga
    14881 catccgggat aaagaatttc tacgttccgt atttgaaaag gaaccgattg aaggagtaat
    14941 ccattttgct gcaagctctt tagtaggaga atctgtcgaa aaaccattga tgtatttcaa
    15001 caacaacgta tacggtatgc aaatcttact tgaagtcatg cacgagttca atgtgaataa
    15061 aatcgtgttc tcttctactg cagcaactta cggagaacca aaagagtctc caatcacaga
    15121 agatacacca gctaatccta aaaatccgta tggtgaaagt aaattgatga tggaaaaaat
    15181 gatgaagtgg tgtgaccaag catatggcat gcgctatgtc gctttgcgct acttcaatgt
    15241 agccggagca aaagcagatg catctatcgg agaagatcat acaccagaaa cgcatcttgt
    15301 cccaatcatt ttacaagtag ctcttggtca aagaaaagca ttggctgttt atggagacga
    15361 ttatgataca ccagatggaa catgtatcag agattatgtt caagtagaag atttgattgc
    15421 tgcacatatt ttagctttgg aatacttgaa agaaggaaat gaaagcaact tcttcaattt
    15481 aggcagcagc aaaggatact cagtcaagga aatgctagaa gcagctagag aagtgacagg
    15541 aaaagaaatt ccagcagaaa tagcaccaag aagagctgga gatcctagcc gattagtcgc
    15601 ttcaagtgaa aaagcgagag aggttctggg ttggaaacct gaatacacag atatcaaagc
    15661 aattatcaaa acagcatggg attggcatgt cagccatcca aatggttatg aagaataggg
    15721 gaatgagata tgtcaatcag tcaaacgatt tgtgattttg ccacacttgc gatcgaagct
    15781 ggcggatgga tggaaatgga ccgcttatat ttacagaatc gtcttttagg gatggtagga
    15841 gaagatgctc tagaaagcat tgaagtaaga ccagttaagg aagaatctgt agacttattg
    15901 gatcagcttg tcgctaaggc aagagaaaat ggtgttcttt cagaattgcc agcagaacaa
    15961 gagatttttg aagcgcaatt aatggatttt ctcacaccgc caccttcagt cgtcaatgca
    16021 ttctttgcac agcactattc gaaaagccca gaggaagcaa ctgagtattt ctttaattta
    16081 agtaagaaaa atgactacat caaaacaaga gccatcgcaa aaaacatcaa atttccggca
    16141 gagtcggagt atggtgaact ggaaatcaca atcaatttat ctaagccaga aaaagatcca
    16201 aaacaaatcg cagcagaacg cgaggcggta aaagtagact atcctacctg catgctttgc
    16261 atggaaaacg aaggatatag aggtcgcttg aactatccag cccgcaccaa tcaccggatt
    16321 attcgaatga atctagacgg tgaaagctgg ggattccaat attctcctta tgcttattac
    16381 aatgaacatt cgatcatttt atcagaacaa caccgcccga tgaaaatcac aaaggaaaca
    16441 tttgctagac tgcttcggat tactgaagtc ttgcctcatt actttgttgg ttcgaatgct
    16501 gacttgccga ttgttggagg atcgattctt tctcatgatc attatcaggc aggacgtcat
    16561 gaatttccta tggcaaaagc accgatagaa aaacttatca aactagcaga ttatccgcaa
    16621 gtcatcgcag gagtcgtgaa atggccgatg tctgtgatcc gtttgcaatc aacagagaaa
    16681 aaattgttga ttgaagcagc taactcagta ttggaaaagt ggaaagcata ttcggatgaa
    16741 acagtatcta tcagagcttt ttcagcagat ggtacaccac accatacgat tacgccaatt
    16801 gcacgaagaa gggacaatta ctttgagttg gaccttgtcc ttcgagacaa caatgtttca
    16861 gaagaacatc ctgatgggat ttttcatcca cataaagatg tccaacatat caaaaaggag
    16921 aacattgggt tgatcgaagt aatggggctt gctgttttac cacctcgttt gaaaccagaa
    16981 ctagtagaag tagagcattt cttattagga gaagaaaacc agatggctga atatcatcgt
    17041 ccgtgggcag aacaattaaa aaaagaacat cctgatttga cgaaagatac ggtcaccgat
    17101 gtcgtacaaa aatctgtagg tcaggttttt gcacgtgtac tggaagacgc tggagtattc
    17161 aagcgagatg aaaaaggaca ggcagcgttt gaacgattta ctgcaatttt ataaacgtaa
    17221 aaaaggagga gagacaaatg gcaacaatta aagacattgc gcagttggca ggagtttctc
    17281 ctgctactgt ttcaagagtt ctcaactatg atcctgatct ttccgttggc ttagccacaa
    17341 agcaaaaaat ttttgaagca gcagaacgct taaattatac gaaacatcgt aaaaatgcag
    17401 caaatactaa gcaaacgctt cgcctgatcc agtggtacga cgatacggaa gagcttgaag
    17461 atctttatta tttatctatt cgtctaggaa tcgaaaaaaa ggcagaagaa gcaaacgtta
    17521 ctttattgaa agaaacgtgg gattccatta gtgatcttcc gacagatgga acgatcgcac
    17581 taggaaaatt cgacgaagct cagttattag aaatgaaagg atcccaaaat gccttgcttt
    17641 tggtagattc tgacggcact tctcaaggaa tcgactctct agttgtcgac tttaagtcaa
    17701 gtgttcaaaa agtcattgaa cattttgtct cccataaagc gcaacatatc gctatgcttg
    17761 ctggaacaga atatacgaag aaaaaccatc agcggctaaa agatcccaga attatggcat
    17821 ttcaacaagt tttggcagaa aaaggctacg acgaaggtcc aattatcgaa gcagattttt
    17881 ctgtcgagtc aggatatcaa gcagtgaagc agtatttaaa aacgaatccg catgttcctg
    17941 atgcgctttt tgcagctaat gatgcattag cgattggcgc actgaaagcg attcaagaaa
    18001 aaggtttagt ggtaccagaa gatatttctg tgatgggttt caatgatgtc agtgtagcga
    18061 aatatgtaac gccctctctt tctactgtaa aagtttatac cgaatggatg ggagagctag
    18121 cagtggagac gattcttcaa ttaagtaatt ccaaagcacc ggtttcaaga aagattattg
    18181 tggaaacaga gttgattatt cgagaatcaa cgaaataaa
//
LOCUS       DAYYNQ010000059        17980 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000059 DAYYNQ010000000
VERSION     DAYYNQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.059"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHBJ1_001843"
     CDS             complement(11..1594)
                     /gene="abc-f"
                     /locus_tag="ACHBJ1_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM9430778.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(1699..1827)
                     /locus_tag="ACHBJ1_001844"
     CDS             complement(1699..1827)
                     /locus_tag="ACHBJ1_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430779.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(2172..2315)
                     /locus_tag="ACHBJ1_001845"
     CDS             complement(2172..2315)
                     /locus_tag="ACHBJ1_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM9430780.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="ACHBJ1_001846"
     CDS             complement(2538..3017)
                     /locus_tag="ACHBJ1_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9430781.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="ACHBJ1_001847"
     CDS             complement(3090..3374)
                     /locus_tag="ACHBJ1_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430782.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="ACHBJ1_001848"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHBJ1_001848"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="ACHBJ1_001849"
     CDS             complement(3718..5004)
                     /locus_tag="ACHBJ1_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM9430783.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="ACHBJ1_001850"
     CDS             5142..5813
                     /locus_tag="ACHBJ1_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM9430784.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="ACHBJ1_001851"
     CDS             5779..6267
                     /locus_tag="ACHBJ1_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM9430785.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="ACHBJ1_001852"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="ACHBJ1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM9430786.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="ACHBJ1_001853"
     CDS             7228..7992
                     /locus_tag="ACHBJ1_001853"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM9430787.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="ACHBJ1_001854"
     CDS             8225..10438
                     /locus_tag="ACHBJ1_001854"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM9430788.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /gene="dtd"
                     /locus_tag="ACHBJ1_001855"
     CDS             10449..10895
                     /gene="dtd"
                     /locus_tag="ACHBJ1_001855"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM9430789.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="ACHBJ1_001856"
     CDS             11066..12325
                     /locus_tag="ACHBJ1_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM9430790.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="ACHBJ1_001857"
     CDS             complement(12414..13361)
                     /locus_tag="ACHBJ1_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9430791.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /gene="hisS"
                     /locus_tag="ACHBJ1_001858"
     CDS             13755..15047
                     /gene="hisS"
                     /locus_tag="ACHBJ1_001858"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM9430792.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="ACHBJ1_001859"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="ACHBJ1_001859"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM9430793.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="ACHBJ1_001860"
     CDS             16940..17815
                     /locus_tag="ACHBJ1_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9430794.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
BASE COUNT         5683 a         3342 c         3841 g         5114 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat
//
LOCUS       DAYYNQ010000060        17529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000060 DAYYNQ010000000
VERSION     DAYYNQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.060"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..421
                     /locus_tag="ACHBJ1_001861"
     CDS             191..421
                     /locus_tag="ACHBJ1_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM9430795.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="ACHBJ1_001862"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="ACHBJ1_001862"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM9430796.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="ACHBJ1_001863"
     CDS             1832..2008
                     /locus_tag="ACHBJ1_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9430797.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="ACHBJ1_001864"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="ACHBJ1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM9430798.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="ACHBJ1_001865"
     CDS             2624..3091
                     /locus_tag="ACHBJ1_001865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430799.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="ACHBJ1_001866"
     CDS             3176..4060
                     /locus_tag="ACHBJ1_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM9430800.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="ACHBJ1_001867"
     CDS             4174..4410
                     /locus_tag="ACHBJ1_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM9430801.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="ACHBJ1_001868"
     tRNA            4498..4578
                     /locus_tag="ACHBJ1_001868"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="ACHBJ1_001869"
     tRNA            4585..4656
                     /locus_tag="ACHBJ1_001869"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="ACHBJ1_001870"
     CDS             4781..4951
                     /locus_tag="ACHBJ1_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430802.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="ACHBJ1_001871"
     CDS             5171..5878
                     /locus_tag="ACHBJ1_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9430803.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="ACHBJ1_001872"
     CDS             complement(5937..6581)
                     /locus_tag="ACHBJ1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM9430804.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="ACHBJ1_001873"
     CDS             6707..6994
                     /locus_tag="ACHBJ1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430805.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="ACHBJ1_001874"
     CDS             7102..7422
                     /locus_tag="ACHBJ1_001874"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9430806.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="ACHBJ1_001875"
     CDS             7503..8816
                     /locus_tag="ACHBJ1_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM9430807.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="ACHBJ1_001876"
     CDS             8813..9532
                     /locus_tag="ACHBJ1_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM9430808.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="ACHBJ1_001877"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="ACHBJ1_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM9430809.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="ACHBJ1_001878"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="ACHBJ1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM9430810.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="ACHBJ1_001879"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="ACHBJ1_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM9430811.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHBJ1_001880"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="ACHBJ1_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM9430812.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHBJ1_001881"
     CDS             14978..15862
                     /gene="ftsX"
                     /locus_tag="ACHBJ1_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM9430813.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="ACHBJ1_001882"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="ACHBJ1_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9430814.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5858 a         2962 c         3802 g         4907 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccattt
//
LOCUS       DAYYNQ010000061        17363 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000061 DAYYNQ010000000
VERSION     DAYYNQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.061"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..493
                     /locus_tag="ACHBJ1_001883"
     CDS             56..493
                     /locus_tag="ACHBJ1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430815.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            588..1472
                     /locus_tag="ACHBJ1_001884"
     CDS             588..1472
                     /locus_tag="ACHBJ1_001884"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9430816.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1696..4863
                     /locus_tag="ACHBJ1_001885"
     CDS             1696..4863
                     /locus_tag="ACHBJ1_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM9430817.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4931..5428
                     /locus_tag="ACHBJ1_001886"
     CDS             4931..5428
                     /locus_tag="ACHBJ1_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM9430818.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5447..5911
                     /locus_tag="ACHBJ1_001887"
                     /pseudo
     CDS             5447..5911
                     /locus_tag="ACHBJ1_001887"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5908..6666
                     /locus_tag="ACHBJ1_001888"
     CDS             5908..6666
                     /locus_tag="ACHBJ1_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM9430819.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6752..7492)
                     /locus_tag="ACHBJ1_001889"
     CDS             complement(6752..7492)
                     /locus_tag="ACHBJ1_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9430820.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7553..8806
                     /locus_tag="ACHBJ1_001890"
     CDS             7553..8806
                     /locus_tag="ACHBJ1_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9430821.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9128..9355
                     /locus_tag="ACHBJ1_001891"
     CDS             9128..9355
                     /locus_tag="ACHBJ1_001891"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM9430822.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9478..9804
                     /locus_tag="ACHBJ1_001892"
     CDS             9478..9804
                     /locus_tag="ACHBJ1_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM9430823.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9872..10585
                     /locus_tag="ACHBJ1_001893"
     CDS             9872..10585
                     /locus_tag="ACHBJ1_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM9430824.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10895..11745
                     /gene="licT"
                     /locus_tag="ACHBJ1_001894"
                     /pseudo
     CDS             10895..11745
                     /gene="licT"
                     /locus_tag="ACHBJ1_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            11866..13731
                     /locus_tag="ACHBJ1_001895"
     CDS             11866..13731
                     /locus_tag="ACHBJ1_001895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM9430825.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13745..15188
                     /locus_tag="ACHBJ1_001896"
                     /pseudo
     CDS             13745..15188
                     /locus_tag="ACHBJ1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15296..15997
                     /locus_tag="ACHBJ1_001897"
     CDS             15296..15997
                     /locus_tag="ACHBJ1_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9430826.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16123..16635
                     /locus_tag="ACHBJ1_001898"
                     /pseudo
     CDS             16123..16635
                     /locus_tag="ACHBJ1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(17033..17242)
                     /locus_tag="ACHBJ1_001899"
     CDS             complement(17033..17242)
                     /locus_tag="ACHBJ1_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM9430827.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
BASE COUNT         5801 a         2911 c         3727 g         4924 t
ORIGIN      
        1 tgtataatct taaataaaga aaacaatatc aaaaaatgca cgtagaagta gataaatgga
       61 gtggttttgc aaatttcttt tatttctaga agttattcac gtatacggat ttgtttttct
      121 cacaaataaa aaggcaggaa gtacaatgga tcatttgtat ataaaatata ttcatcaatt
      181 ttccgttttg gtgttaggca cactaattat agcggggatt gtcgcattta ttattcaagg
      241 ccgtgacata ttaacagtac atcaagagtt tttcagttat gcatttggta ttcctctgat
      301 cgctataagg tctgataaca aaaagtggaa gaaacgctat tacattctct tgctattttt
      361 gtgtttttat ctgccaccta agatttttac tttattaggt atacaatgcc tatcattttc
      421 ttttatttcc tatatcattg ttttaatttt cctcagtttc atagtatgga tgttttatct
      481 tgtcaaaaaa taaatgaatg tttcatgaac agtacttcat cacagcctaa tttttttctt
      541 tatgctatcg ttatattaaa gaaatcaatc agaagggaaa gaaagtgatg aaaaaagttt
      601 tattagtggt aaaccccagt tcaggcggtg aacaagcaaa ggaatttgag caattagcga
      661 tagcaaaatt ggaatcggtg tttgatgaag tggtcgtctt gcatacaaaa aaagcagggg
      721 atgcaaaaaa ctttactcgc gaagcggcta cggaagggta tcatagtgtg tttgtcatgg
      781 gcggagatgg aacagtcaac gaaggaatca gtggaatcgc tgaacaagaa catcggccaa
      841 atttcggatt tttcccattg ggtacggtga atgacttggc tcgagcatta gggataccgc
      901 tagaacctga ggaagcgatc aatcattttt cgattgaatc agtcaagcca ttggatattg
      961 gaaaaataaa tgacgactat tttatgaatg tcgtcgcaat tggctctatc cctgaatcaa
     1021 tcaatgatgt agatgcggag aagaagacga aatttggaaa actagcttat ttcatgtcag
     1081 gaatcaaaca gttagcttcc acacaatcct actcgtttca cgtagaagtt gatggaaaga
     1141 aggaagaaat tgagagcagt accttgctga taggactaac taactcagtc ggcggttttg
     1201 aaacattact tccaaatgca aaagtggatg atggtaagct ccatttggtt tatttaaaag
     1261 attcttcttt gatcgatacg cttaaaacat tgccagacct attaaaagga gtagatgaat
     1321 ctacgaaaaa tctagcctac cttacttgtg agcaattagt tgtgtcatta gctgatgagg
     1381 aagaattgac gacgaatgtt gatggagatg aaggggataa attacctgtg acaatcaaag
     1441 tattgccctc acatttacag gtttattgtt aaaatggagg ttgtgaccga ggagttggcg
     1501 ggagaacacc gcttgttcgg gataagggac tgtgacaaga cttttgtcac agcctttttt
     1561 ttgtacaaaa aagaaattta cacctaatag aaaaattttt ccataaaaat acatatttta
     1621 aatatgaaag tacaattgga aatagattaa aacgataaaa taaaattgtc aaaacgtcag
     1681 agaggatggt ggagaatgaa tcaatgtgta aaaaagctga ttgcgtcgat tttgtttgtc
     1741 ttccctctct tgatggtgac atccgttttt gccgatgtag agaaaacaaa cgaatcaacg
     1801 caatcagaga atcaaagttt tgacaagact actttttcag aagaatcgat tacttctgaa
     1861 actacagaat ctcaaagtga ggcttctact aataaaaggc aagaagaaaa taagacaagt
     1921 gagacctcag aagtagctga acaaacgtct gatagcagtg aagcggcact ggcaagagct
     1981 gtttcagcgg atgggtacag cgaagctgcc acaacagaag aactggcgca gcttctcgca
     2041 gacgaatcag taactaagat ccgactgatt cagcctttga ctttagacag ggaattagag
     2101 atcaaacgag atatcgttat tgattttggt ggattcgctc acaatttcgg cacacaccat
     2161 atttatatca atgagatacc tcagtcgatc gagttccaga attttaaagg aacagctgca
     2221 catccaggtg gattgacacc aaatatcgat ggaaatgcca ttatcattgc ttatatgacc
     2281 gatttttggg gaagcagata tcattttacg ggagaaataa aatttaccgg gacacttgat
     2341 ttgtatgatg ggtccaaatt aggactgatt tacgctcctc gtgcaactgt cacattagat
     2401 ggtgtttccg gtgtgttgga tgtccaacca gtgaaggaag gactgaatgc cgctccgggt
     2461 aaagcctatt tttgtagaag ctatcaattg aatgtgatca atggtagtca actttatgga
     2521 ccgtatctag ggaaattcta cggttttcta gattcaggag atgagactgg tagtggaaaa
     2581 acagcgccag gaatccatat cttaagcggt tcaaaggtct ctcttgatta tgatcgggca
     2641 gatggcgtta gcgatgaagg agaggcagtc gatacgctac ctggtaatgt catgtttgaa
     2701 gtcagtggtt cgggaagcga attttcggta aatacaaaga tcaatattac caatgacaac
     2761 aatcgcggaa tcatccaaat gagaggttca ggatcgaaag tcactgtatc gaatggtggg
     2821 aaaatcacca tcaatacagc aactacgggc ggatttcgct tgcaaggaga ttctagtcaa
     2881 atccatgtat caagtggtgg acaaatctcg gtcaaaatgg ctggagatga cgatcaaccc
     2941 gggaacaatg ggatgcgttt tgtaggaaaa ggcctttctt tgatcgtaga aggagccgga
     3001 tcagttatcg acatcgataa gcagagtggg cgttccagcg cagttcgttt tgagaatggt
     3061 acgcaaaagc tgacagtaac caatggaggc tcattgaggg taagaaatac aggtgatggc
     3121 aaatcttacg tgaacagact caatcaagga aatcaagcga tccaatttta tgacgcttct
     3181 ggttttctag aaagtccagg ttcagctgat tttacagtca ctggagaaaa atcaaatatc
     3241 gatatacgcg cagattccgg agcaactgta gatacaactg gagatattga tttaaatttt
     3301 acggcaggtg aaaaaactta tttagtcatg gtcgggaaaa caggggaaga taagaccggc
     3361 atcatcagtg gggatgtgtt gaatgtggac ttagaatctc ctacctattt tgatttccaa
     3421 aacaaacgga ttggaagcga gaccaatggc ataggcggct gggtattcca aggaaatgga
     3481 gattcttctt taaagatcgc ccattcagaa atcgctttat ggcgaaaaac aggcgcaaca
     3541 ttcgataact ttgaaaaaga cccggattat ttttctgata tgacagactt atcgatcaat
     3601 gggaccgact taggcaattt cgtatcaagc cctagtgcag gtttaacaaa tgaatttgct
     3661 cagacaggtg ccggaatgaa aaattataat cgaatcagtg ccaataatca gctcccgatg
     3721 attgaatcct taagagtgcc aacgaatgca gataaaaaaa tctatgggca tgtcgttgtt
     3781 cccgaaggtg tcgaggcaaa gccaagagat gcttggacag gcgaagtaga agtgacttta
     3841 cgtattactt acgcagatac gacgaaatca tctgtagaaa ttactgctct cacgcaagga
     3901 caaacagatg aaaatgaagg ggcaggatat aacccatggg gagaaggcga acaaggcggc
     3961 ttgttcgaag ttcaagtacc caatgatgag agattaaaaa cggaggatca agtgcaagtt
     4021 gttgctgcta aaaaagtaag aggtggccaa gaagtgacag aactggactc tgccaaaacg
     4081 acagtcgatg tgattcctcc tgaacctgct aatttaagta ccacattgat caatgcagct
     4141 tctcgcacga tcacaggtac aggtgaagca ggagccttgg tttcattgaa aaaaaatcaa
     4201 gctgttttgt cagaaacaac tattgatgat acaggggaat ttgttctttc tatcccagaa
     4261 aatcaacttt ttgcaggaga tgtattagaa attgtattaa acgaccaagc aggagaagct
     4321 actgctaagg gtgtgcagaa caagccaagt accaacgatg aaattgggaa tcacaatcca
     4381 agtgatacgc cagttgttta tcatgatgcc ccgccttttg cacctgctac gaagatcgta
     4441 gtagaaggtg gtctctcatt tcttgcccct tccaaactcg attttggtca gatcaaagca
     4501 actggtttga cacaagaagc atttggccaa atggatatac aggaaaaatt atggatttat
     4561 gatcagcgag aaacgaaaaa tccgtggatc ttaacgataa aattatctca gcctttcaca
     4621 aatgatgcag gattttctat gcaggatgtc ctatatttca agaaaggaga ggagtatcaa
     4681 ttagtgaatg aagaagaact ggaaattgtc agaggacaaa attcaacgaa tgtgcaagca
     4741 gattattcag aatatttaaa taacggtcgg gcgtttaaac ttgtcttaac caaagaaaat
     4801 cagatagctg gtaattactc agcagagata acatggacat tagcagatgc gccgacagaa
     4861 taaagatcaa ggaggatgat tgatgaatcg aatatggaat aaaagtcatc gaaacaactg
     4921 ctctttactt atgatccttc tccttggatt catcctttgg cagacacctg tccatgcctc
     4981 cgaattcaat tttgcggtag attttgaatt accggctaat cagattgata agaacaaaac
     5041 atatataaat gtaaggatgg agcctgggaa aacacaggaa atcccctaca cggtctttaa
     5101 ccgaacagat aaatcagtaa cagttgagac attgatccac tccgctacga cgaattcaaa
     5161 cggtgtgatc gaatatggag aatcaaaaag taaatttgat gaaacattaa gttatgatat
     5221 gaaaaactta gcaaaaacag tgggagaaat aactgttcct cctcaaagtg cggtcactga
     5281 gacactttca ctgaaagcgc cagaaaaaga gctggctggt gtggtcgcag gagggatcac
     5341 gtttcaagaa aaaaaagaga acgtttctaa caaaagtaca gttggtgtcg tcaatcagtt
     5401 ttctcaagtg aaagcaatct tgatttgaaa tagcgaaaaa gagtcttccc cagatttgaa
     5461 attaaaaaaa gcttatgcgg atcaaagcga tggaagaaat gtgattaagg cgaatcttca
     5521 aaatccaaat gcttcttata tgttcggctt gactacatcg gttgaaatct acaaaaaaga
     5581 aacaaaagag ccttaccttt caataaaaaa agaaaaaata gatgtagcac ccaatacaaa
     5641 ttttgattta cttgtcccat tagaaggaaa aaaattagct ccgggacatt atcaagcggt
     5701 tgtgaagggt gcttggaaag aaaaacgctg gaatctccat accgattttg aaataactga
     5761 tgatgcagcg gaccaattga atgaaaagga tattgattta aaaaatcagc cagaaacatt
     5821 tgactggcga tggctgatac ttgttggagt cattcttatt ggcttatttg ctggattatt
     5881 tattttaaaa gaaagaagga aaaagaaatg aaaaaaacaa ttgcattaag taccgttcta
     5941 gctggaagtc tattattatg tacacaaagt gtttttgctg agaataagag ctcagccact
     6001 acagaaggaa cgatttctgt gaccaaacca gatgctgcaa atcctgaaga cgttgttcca
     6061 gttcctcctg ttgatccaaa tgatcctgat actcctttta cacctgacga accaaatcag
     6121 ggaacatccg gattactgac gattgaccaa gctccagatc ttgattttgg aacaatcaag
     6181 ttaggtacag caaaaacagt atatgctgca ttacaaagcg gaacggacag tactggagca
     6241 caaacacaag tacagaattt cgtacaagta acggataaaa gcggaaatta tgccggttgg
     6301 aaattatcag tcaaacgaac agaatttatt ggtacaacat ctgatacgaa taaactaact
     6361 ggttcaaagg tgaaattcaa aaacgccgtg attcgaaata gcgcaactaa tagcagtgga
     6421 atgccatcta ccgtttctgg aactggcgct gaaggaatcg aaatcccagc agatcaggca
     6481 gtagatctag taactgcagg agcaaatgaa gggattggca catggattta ttcattagga
     6541 gagaatgtgg aacaagggag acaatcagtg gaattatttg ttcctgtatc caattatgct
     6601 gtagacaact acgtttcaac attcacatgg tcgttaacag atgctcctac agaagaggaa
     6661 gcgtaaaatc atatcaacga tttcagggag tggagccgat tcaaggccca ctccctgaaa
     6721 tctgctatgc ttacgtctca gctcttattc atcaactcga agcagttttt tggaagtcat
     6781 ggaaacgttc ctttgtcttt tcaaagatct ttcgaccaac gagtaaggag tcaccgttga
     6841 aaaattctaa cacataatga gcagaatcaa ttttctgcaa ttgcgataag ttgatacagt
     6901 aagattttaa aagtaaaaag taagcatact tttctaactc tgtcttcaat tgttttaaag
     6961 aagtattcac taatttatct tctgtagttg ttttgaccat aacttggttt ttcaaacctt
     7021 tgatactttc aatatataaa atttcccgag gatacaggac gagacttcca cctttttgat
     7081 caataaagat tttatcgaca tttacgatct gctgttcttt ccaattgatc tgttctagca
     7141 cttgacgaat ttgttcagat aaattcctgt cttttctgat atatccaatc ggaaggattt
     7201 tccgattgat gatctctact gcaaaattcg tataagcggt gaaaaaaaca atattactat
     7261 gtgcattttt tttacgtatt tgttcggcga catcaatacc tgtgagtgat tgcttcaagt
     7321 cgatatcgat aaagaaaaca tcgtgattat caatagtgcc caactggatc aactgattga
     7381 tttgagagat agaagtgagc tgcacgatat tctcgatatg atgattgacc aattctctga
     7441 tgatccattc gctgtgaaac tggttatctt ctatgataaa atactttttc atatgcaggc
     7501 tcctattaaa aaaatatgtg tacattttga aacaaaacaa tggaggaata agatggtcgc
     7561 aacagtgaca ctttatttga cctttacttt gattttagat ttaggtatct atttattctt
     7621 ttcgaaacaa ctgctagtaa aaaacagctt tggttgtata gctttgatgg tggtgacact
     7681 tttacttcct atccctaaag tgagctttcc ttacctagtt ggcttattat tgatgtttct
     7741 tttttatttt actacgaaaa acctgtttat cagctgtttg atcagtaatc ttttttttgc
     7801 tatcactgga ctcagctttt ttctggtttt tgatctgatg gattatttag gaaagagaca
     7861 tcttggaaca gcaagttttt ttgcactact tatcgcattt attttctatc tattagtaaa
     7921 aagtatagac aaacagacga gatttctcaa cttgcttttt acttatccaa ctgaacacta
     7981 ttttggaggt atttgtattt tattaagctg gggcgtgatt tacttggtat tgggaagtgt
     8041 tcacttccgc tcatctaatt atctgcttct atctcttttg tcgatagtca tcagtttttt
     8101 ttctttgttt ttttccatta tgttgatcag tttccaagta aaagaaagac aacatcagga
     8161 atcgcttcaa ctgtatcaaa taaatgaaga atattaccat catctagagg aattcaaaca
     8221 tgattataaa agtctactgt tttcattaaa aattactctt caggaaaata aaaaagaaac
     8281 gttggaattt ttagaaatat tagaagatca ttctgcagct atttttgaaa aaccgcaaat
     8341 acaacagcta acaaatattg tatccccagc tgtcaggggt gtctttttaa aatttctaga
     8401 aaaagcagaa tcggagaaaa tcccgatcca agtagttatt cctgtcgaag tgaaaacaat
     8461 cccgattgat ctggttgttt ttattcgctg tttatctatt tttatttcta acgcattcga
     8521 tcatcgtgta gccgatcaga gtccgattac gatcaatctg ctggaagaaa aagagggatc
     8581 acgttttagt atagcgaaca aggcaaatcc aacgaatttg acgctatcgg agatggtcaa
     8641 aagaggaaaa acctctaaaa aaaatggcgg gttaggtcta tacagtgcga aaaaaatgtt
     8701 ggatgtttac gaaaatgcag aactaaaaat cgaattttca aaacagaatc actatttttt
     8761 tgtagaaatg tatctggcat ctagaaagag caaatcagcc tactgactta ctgtcaaagg
     8821 ataagtttga ggaaagtcta cacggagata gagacaacaa cacagcatat tcaaaagaaa
     8881 cagaagcgtg atgaccgttg tcacccttct gtttcttttg ctatgtagtt aaacaaagta
     8941 gttgacactt ccgctactta gtattagtac gaaagtatac ttcatgatac taagtagata
     9001 agaagtgaaa agcggaaccc acgatcgtca aaatgctaac tgctttgctt acaccagata
     9061 aagggaagag caatatcgct tattttaggc gtggagaagc aagcaaatcc ctacgatcac
     9121 aaaaaaaatg ctgacccagc agttaagaga gttagaacaa gatgaaagta tccatcgaaa
     9181 agcctataac caagtcccgc ctaaagtaga gtactcgctc accgaagaag gaaaatcatt
     9241 aagagagatt ctagtagcgt tgtctgtttg ggaagaagaa cgtatcgaaa aacaaaaagc
     9301 ctcaagggaa gaagacacgc tactgcatac acatggtggc tacctaaatt attgattttc
     9361 tatgacattt attttaaata aaagaaaaca ggagtttttt taaaagcatc atctattttt
     9421 tatgtaaaaa aatggtatga ttaatcaagt aagaagtgat tggaagaagg caagcaaatg
     9481 aatagtatgg aaaaagtcaa ccagtttaga gatgaacgta attggcgaca atttcataac
     9541 gaaaaagatt tggctatctc tatttctttg gaagcatctg aattgttaga actttttcaa
     9601 tggaaaacgc cggaagaagt aaaagaaaca caattagaac gaatcaaaga agaactggca
     9661 gatgtattga tctattcata catgatggca gacaaccttc acctggatct agatgagatc
     9721 attgaagaaa aattgatcaa aaataatttg aaatatcctg tagaaaaaag cgcaggtgta
     9781 cgaaaaaaat acacagagtt gtagaaatgt gtcattcggc tgttggaaag agcaaaagaa
     9841 aaacagtaaa atgagtctgg agggagagaa aatgaaaaaa aaaggaaaag caaaaagatg
     9901 gctagtcaat atcctcttgt tcctcttgtt attagtagga ctagctttga ttttcaatga
     9961 acagatcaag gattattttg tcagagagac aggagataaa tatgctatag cgaatgtaac
    10021 aaaagaagat ctgaaaaaga ataacgataa agatgtcagt tttgactttg atgcggtaga
    10081 accaatgacg acagaagggg tcatgaggtc gcaaatgaga ggaacagatc taccagtgat
    10141 tgctagtatc gcagtccctt cagtttcaat caatttaccg gttttcaaag gcttggataa
    10201 tacgtcacta ctttacggag caggaacatt atctccagat caagaaatgg gaaaaggaaa
    10261 ttatgcctta gcaagccatc gtgcaacgaa ccctgaattg ctgtttacac cgctagaaaa
    10321 tttagagatg ggtgccaaga tctatttgac agatttagaa aatgtgtata catacaaaac
    10381 attttttaaa gaaaaagttg ctcctacaga tacccaatta ctgaatgaag tcgagggaaa
    10441 agaaattgtc acactaatta cttgtggcga catggacgcc gtcacgcgac tagttgtaca
    10501 aggagaactg gaaagtgtta catcgataaa agatgcgaca gatgatatga gatctgcttt
    10561 caatctagag acaaaaacat tttaggatac tgggacataa gtcgttacag attaaaaaca
    10621 gccaaaggat atgaatcgat aaggaaaaac caatttgctg aaaaaaagag aaacgcaaag
    10681 aggtgcatct ctgatttggt tgttttctag atagacttgt gttccagttt ttttattttt
    10741 tatatgaaaa ggcttgcata aataaaaatt cgatggtatg atgattttga ataagaaatg
    10801 gattgttact gtacggcagg caaaaccaga attcagctaa gaaacagtag gcgaattcta
    10861 ggttttttta tttttcttga gagggcgaag caaaatgaaa attgataaaa ttttgaataa
    10921 caatgtcgtc atttcgaaaa atggattcgg ggaagaagtc gtttgtatgg gaaaaggact
    10981 agcttttcaa aaaaagattg gggacgagat ttcgccagaa gcggtccaaa aagaatttgt
    11041 ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat tccggcagag
    11101 gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa agttgaattt
    11161 tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac acgagcaaaa
    11221 gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta tccgaaagaa
    11281 tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt ggaactttct
    11341 gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg aaacgaagac
    11401 atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat catcagtaaa
    11461 ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat tactcatctg
    11521 cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga tgaatttctc
    11581 tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg gatcaatgag
    11641 tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta tttaacgatt
    11701 catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt gttactgtat
    11761 ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg tttttttcaa
    11821 tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga taatcaagca
    11881 gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag tttagtacat
    11941 tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac gcaaaagtta
    12001 aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca agtagtgatt
    12061 ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct tgcagtttcg
    12121 gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat tatctctagt
    12181 atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg attcttatca
    12241 ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat attgtttgct
    12301 gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc agctaaaaag
    12361 ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta tccagctatc
    12421 acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt gattctttca
    12481 ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt tcaaagtaaa
    12541 ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt agttccgctt
    12601 gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat cgggacggtt
    12661 gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc aatcgtagca
    12721 ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca ttggggattc
    12781 gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat gccaatgctt
    12841 cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg aacaaaagat
    12901 acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg aatcactgaa
    12961 ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc ttgtatttct
    13021 ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc cagcagtttg
    13081 gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga gtcaaatgtg
    13141 acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg tacattgatt
    13201 ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc gaatgcagga
    13261 gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa agtactgcca
    13321 ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg tatcgctatt
    13381 gatccagaag taggagaact agttgcacct gcagacggag agatcacgac tatttttcca
    13441 actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat tcatatcggg
    13501 atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca aggggatctc
    13561 gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc agctggctat
    13621 agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat acttgaatta
    13681 gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata aggaggaaat
    13741 caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg ctgccaaatc
    13801 aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat gctatgcctg
    13861 gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg attgatcaag
    13921 aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc aaagaagatc
    13981 ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc tggacacgaa
    14041 tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt tatgatcagc
    14101 tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct cattacgaaa
    14161 tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg atcgacttct
    14221 atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa tattggatga
    14281 ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga ttagtaaaaa
    14341 gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa ttcgttgcaa
    14401 gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc ggctgcatga
    14461 ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca gcaacaatga
    14521 tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga gaataccctg
    14581 catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta gagcaaacgg
    14641 aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt agttattata
    14701 tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg aacttgctgg
    14761 gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc gatccagaag
    14821 ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta ttcattgttg
    14881 aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt gatgattatc
    14941 ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca gacggtgtag
    15001 atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca acaggagaaa
    15061 tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat gggacactga
    15121 atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca aatggcgtaa
    15181 acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga tttgtgttct
    15241 ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg acgttatgaa
    15301 aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata ttttctcagg
    15361 aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac tgtttcaagt
    15421 cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat atgtcgcaaa
    15481 gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat tatccaaagc
    15541 ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag aaacgctgga
    15601 tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg gtgagcaagc
    15661 tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt ttgttcatta
    15721 tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat ctttgtatcg
    15781 tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct cggcagtatt
    15841 tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca tcaaacggat
    15901 acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg tctaccatac
    15961 ggagctgcat ccttaccata ttgattatga aacataaata aaaaaagagg ttgtgaaaaa
    16021 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcgtt
    16081 tggttaggct tattaccgca gatacacggc tgtttctgga ttaccattta ttctggataa
    16141 gggggtgtgg cacgattttc agaaggaatc tatgaaccaa tcactgtatt gattttagct
    16201 tcgatggtaa ccccattcgg cttgacgatc gcttatttct tagggaaaat cattcgtaaa
    16261 aatatactca atcgtcaaga aatcgataca ttgaagaccg cttttcctat ggggatctgt
    16321 cagatcacag agggatgttt ccctatcgta ttgaacgact tggcacgcaa tgtcatagca
    16381 acgggagtcg gtgggacagt aggtggcgga ttaagcatgt tttggggcgc agatagccgt
    16441 attccggcat cgggaatgtt tgcagtcgct acgatgacca gaccatgggc attcattggt
    16501 gcattgttag ctggttcttt tgtcacagga ataaccattt tattattgaa aaaaccagta
    16561 gatccgaatg cagaaatcat ccaagaagaa aaagaggaag aagacatatc ttgggatgat
    16621 ttgacaatta gttaggagag gcagaatgga ttacaagaaa aaagtacaat agatgtacct
    16681 tgagacagga atggtatgaa agaatcagag ttagaaagaa tcgaatatac agattttggt
    16741 ttaggagcga tcgaaactgt ccagagcatc ggcaccctcc ttgaaatgac gatgaaggaa
    16801 aaatgagaac ctttcgttgt cgaaagggtc tggtatacct tatgtcgaag gaaaggaaac
    16861 aaaaaaactt tcctgtacta ttccaaaaag gaaagaaagt tattatacag taaaaaaaga
    16921 aattattctt catgctagag atcagtacac gttcgagccc aatatcaaac actagttcca
    16981 ggcaggcgca acaggtgcgg ttatcactga attttcttcc agcagtgacg attcaagtga
    17041 tatttttaca aatccagcta tttatcgtta acaaaaagat ttttaggaga agctgatgaa
    17101 caaaaatgat caaattttga tcgtttagtt cgtcggcttt tttatttaga gaaacatcat
    17161 ttatcagtga aacataacac gagaacaaaa tgattttttc gttaataaaa gaaacaagta
    17221 tttgttgttt aaaataatac acgttattgg catataggac gaaatcccag tatcttctat
    17281 aagtaagtca tttcatatcg atgaaaacac gttatttatt tttgttttat ctataaaatg
    17341 gctgaaaaat gatttttttc taa
//
LOCUS       DAYYNQ010000062        16820 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000062 DAYYNQ010000000
VERSION     DAYYNQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.062"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..540)
                     /locus_tag="ACHBJ1_001900"
     CDS             complement(28..540)
                     /locus_tag="ACHBJ1_001900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM9430828.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(554..2851)
                     /gene="recJ"
                     /locus_tag="ACHBJ1_001901"
     CDS             complement(554..2851)
                     /gene="recJ"
                     /locus_tag="ACHBJ1_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM9430829.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(2993..3436)
                     /locus_tag="ACHBJ1_001902"
     CDS             complement(2993..3436)
                     /locus_tag="ACHBJ1_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM9430830.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3437..4225)
                     /locus_tag="ACHBJ1_001903"
     CDS             complement(3437..4225)
                     /locus_tag="ACHBJ1_001903"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9430831.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4222..5163)
                     /gene="rnz"
                     /locus_tag="ACHBJ1_001904"
     CDS             complement(4222..5163)
                     /gene="rnz"
                     /locus_tag="ACHBJ1_001904"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM9430832.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5340..5927)
                     /locus_tag="ACHBJ1_001905"
     CDS             complement(5340..5927)
                     /locus_tag="ACHBJ1_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM9430833.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6148..7461)
                     /gene="obgE"
                     /locus_tag="ACHBJ1_001906"
     CDS             complement(6148..7461)
                     /gene="obgE"
                     /locus_tag="ACHBJ1_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM9430834.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7749..8759
                     /gene="gap"
                     /locus_tag="ACHBJ1_001907"
     CDS             7749..8759
                     /gene="gap"
                     /locus_tag="ACHBJ1_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9430835.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8844..9779)
                     /locus_tag="ACHBJ1_001908"
     CDS             complement(8844..9779)
                     /locus_tag="ACHBJ1_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9430836.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            9965..10924
                     /locus_tag="ACHBJ1_001909"
     CDS             9965..10924
                     /locus_tag="ACHBJ1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM9430837.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(11010..12104)
                     /locus_tag="ACHBJ1_001910"
     CDS             complement(11010..12104)
                     /locus_tag="ACHBJ1_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM9430838.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            12293..13054
                     /locus_tag="ACHBJ1_001911"
     CDS             12293..13054
                     /locus_tag="ACHBJ1_001911"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9430839.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(13169..13609)
                     /gene="fabZ"
                     /locus_tag="ACHBJ1_001912"
     CDS             complement(13169..13609)
                     /gene="fabZ"
                     /locus_tag="ACHBJ1_001912"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM9430840.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            13829..14584
                     /gene="fabI"
                     /locus_tag="ACHBJ1_001913"
     CDS             13829..14584
                     /gene="fabI"
                     /locus_tag="ACHBJ1_001913"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM9430841.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(14694..15746)
                     /locus_tag="ACHBJ1_001914"
     CDS             complement(14694..15746)
                     /locus_tag="ACHBJ1_001914"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM9430842.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            15872..16792
                     /locus_tag="ACHBJ1_001915"
     CDS             15872..16792
                     /locus_tag="ACHBJ1_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM9430843.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         4888 a         3630 c         2946 g         5356 t
ORIGIN      
        1 acatcaagaa tagcttcttt tttctgctta atattccatt aatgtaatga tgtcatatcc
       61 tttgattttg tcgcgtccat ggagatccat caattcgatc aaaaatgcgc agcctacgac
      121 tacaccgcct aattgttcga tcagttcgat cgtcgctttg attgttcctc cagtagctag
      181 caaatcgtca caaatcagca cacgttgacc tggtttaatg gcatccttat gcaatgtcaa
      241 tgtatctgtt ccatattcta atccataagt cacttcgatg gtttctcgag gtaacttccc
      301 ttttttgcgg actggggcaa aaccgactcc taactcataa gcaacaggac atcctacgat
      361 gaatccacgg gcttctggcc caactaccat atcaattcgt ttttcttttg cataatttac
      421 gatttgttta gtagcttccc gataagcgtc accatctgcc atcagtgggg aaatatctcg
      481 aaacacaatt ccttttgacg gatagtcggg aatactggca atataatctt ttaaatccac
      541 tctttgtttc ctcctaggtt gttaaccatt tctttatggt aggaatatcg ctcaaaagca
      601 aaaattcctc aactttgatt ttattgagcc gtcgctggta taatctgctc tcagtcaaag
      661 gatgactttc tgggctagcg actttttgta aaacaccgtt ctctattgta acaaatttca
      721 attcaaaaaa cacctgtatc ataaaaatta ataatttttc agggatacgc aaatattgag
      781 ctattgcttt tattttatga cgtatatcaa ttcgttcttg agaatggatc aatttaaaca
      841 attttgcgta ctgttctctt gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc
      901 ccatcagata aacacgattg aatgaataag accgaaaaac ttctttcaga tccgctaaat
      961 catctggaca atccataaca actagagagt ttggttttct tttttcaatt attgccttta
     1021 cagaatctag tccttcgaaa acaatcgcag cttcctggat aatctcaggc acgaatttca
     1081 acgtcgaaga atcaaatgct aaataaaggc taccttccga cgttcggctc tgttcacgga
     1141 aacgttttgc ccgccaatca aatacttgca gaccacccac agaaaaatca gataccatca
     1201 gctgcggttt cttacgcccg ttccattcat tgatcgaaag ctttcctacc acatctacct
     1261 gattattcag cagttcttct tcttgagtac caaaaccaaa agcgaccgca tcaagctgac
     1321 tcgatgcatc ggaaaggaca aatttcagat gctgttggtt tgctccgatt cttttgatat
     1381 tttctgcttg gacttgtcga aataaaaact gaggcaaagg attatccgta ccaaatggcg
     1441 ccaatagttt cagctcatca attcgctcta ctgttacttc atttggcagc aatacttcat
     1501 caattctcaa ctcaggccct ctcatcaagt caatttgatg atcgactata tattggttca
     1561 tcttctcctg tagtatagtg acattttcac ttggcatcgt taaaccgact gctgcatgat
     1621 gtcctccgaa gtaagtaaat aaatctcgca tctggtcaag catttcaaaa agattcaagg
     1681 cttcaatact acgtccagat cctttagctg atccatcttc ttttaaagta aggacaagcg
     1741 ttggtttacc cgtttcattc attattttcc cagcaacaat gcccaaaacg ccttcatgcc
     1801 atcctcggtt taccaataga tggatatgat tctccttatt gaccatcgct aatgcttctt
     1861 gtgttatctg ctctacgatt gcttttcttt cttcattgat ctcattcaat ttttttgctt
     1921 gaacggatgc ttcctcttca tcaaacgtcg caagtaacga gacggcagga cgtggatcgc
     1981 ccatccgacc gattgcattt agtctgggag caatcgaaaa accgatcgtt gtttcgtctg
     2041 cgtccctcat ttttacgccg ctttcttcaa atagcgcctg aagtcctatc cgttctgaat
     2101 gatgaatggc atcgattcct aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta
     2161 aatcggcgat cgttcctatc gctactaaat ccaaaaattc tgcaggcggt tcttccaata
     2221 atgcagaagc tactttgaat gctactccga ctcctgccaa atctggaaat ggatagtgcc
     2281 ccgctggatg acgagggtga acaatcgcat aagcgtctgg taaattctca ggtagttcgt
     2341 gatgatctgt aatgatcacg tctacaccag cttgctgagc gtatgctacc gcatcatttc
     2401 ctgctacccc gttatcaaca gtgatgatca actggattcc ttcttcgatc ttttcttggt
     2461 ataccgattg gtttggtcca tacccatgct caaatcgatt tggcaggaat acttctactt
     2521 ctgcaccaag taattccagt gtttctttca ttaccgttgc actggtgatc ccatctgcgt
     2581 cataatcacc gtaaacgagg attttttctc cactgattac agcttcttgg attcggctga
     2641 ttgctttatc catatcgtgt atcagatacg gatcatgtaa ttcttgttcc tttggatgta
     2701 aaaatcggtt gatatccttt tcctcatggt aacctctttg ccacagtaac ttaccgacaa
     2761 atggagataa tttcagttca cggatcattt ggagaaagga ttcagaaggc tctgtctcgc
     2821 ttaacttcca atcaaaattt gcttgtctca ctttctcact cctaacttag ttattatagc
     2881 aaaggaaatc agcaaaaaaa agcagaaaga aagaataaaa acagatcaga tccgctagac
     2941 agatgttcag caggacctga tcttttgtac gaccatttat gaaatatccc tattagtcaa
     3001 aataattgaa gctttgtttg ggcgattgag ttttttgttc ttccggcgct ttcaattgac
     3061 tgatttgtgc ggttttcgct tgtaactccg cctgataagc agcttccaac tccgcacgtt
     3121 cgtttctaaa ctcgcgttgc tgttcttctt ttgcttcttc aattttcttt tgaagttctg
     3181 tttgatattc tgacatttcc tgagtcagct gtttgatttg ctttttttgc tgccatagtg
     3241 ttgccgaagc agtcaaaaat acaatcagtg caccaatcag cgcagaaccg atgatcacta
     3301 agatcaatgg cgcagaaaag gaagtgaagc caaaacttac aggaacattt ttattgttca
     3361 atacagcaaa aagaacaata atcaaaacta aaacaaatcc aagaatcaca ttgctttgct
     3421 ttttcaattc cttcacctac tttttgttaa aaattccgct ggctaaataa tcaccaatcg
     3481 ttggaaacag cgtataaaat tttgatgcaa cttctaaaat aaacggttga ttgatttccc
     3541 gttttggatg tctcatattg cggacgatca tttttgccaa cttggcaggt tctaaaacga
     3601 tttgtccgac tttttccaaa tagcttccgc ttggatcagc cttatcgaag aaatttgtct
     3661 cgattggacc tggattcact gtcgtaacag ctacgccaag tggtttcaat tctaatcgca
     3721 aagcgttgga gaagccaagg actgcaaatt tcgtggcaga ataaaccgtt gattttgcag
     3781 ttgccatttt tccagccata gaggcgatat taatgatatg accttgtctt ctttctgcca
     3841 tatcgatcgc aaatttttgt gtcaaaacca tcattcctaa gatgttgact tcaaacatat
     3901 cgtatgcttt tccaaggtcg aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt
     3961 ttaccagaat atcgacgcgc ccgacttctt ccatcacctt ttcatataat tcgtccacgc
     4021 tttcaggatt gctgacgtct accttgaaag cataagcctc tttgccgctc aagcgtctgc
     4081 actcgtctct gacaccttct acaaatgcca ttcttcgtgc gcaacttata acgattgctc
     4141 cttgctttgc tgcttcataa cagatttgtg cgcccagacc gcctgaacta cctgttacta
     4201 aaacaacttt atcttctaaa ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct
     4261 atcaaaatct ttcataatcg tcgtgttttc aaacacttct tgtgcttctt tttcaagctc
     4321 taaagctgct tttcctaaat atctcgcact gatatgagta agtagcagcc gttttgcctg
     4381 cgcttctttt gcaacttcag cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat
     4441 tcttgcttca tctttattaa atgtgctttc atgtaccaga atatcgctat tttccgcaag
     4501 cacaacggaa ttatgtgttt ttctcgtatc gcctaaaata gtcacgattc gtcctttttt
     4561 atctggacct acgaaatctt ttccattgat cgtccgccca tcttctagtt ggatcgtttc
     4621 accttgcttc agtttgccgt acagtggtcc agctggaata cctaattctt ttagacgatc
     4681 cacctgcaat tctcctttat gatcatgttc aaccacacga taaccaaaac tgtcgatccc
     4741 atgatttagt ttttttgtat aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc
     4801 atttagctca atgaatttca gcggataact taaacgcgtt tgcgaaatac ctaaagagac
     4861 ttttatgtat tcctctatcc cttttggccc gtaaatctct aatggcgtat ccccaccttg
     4921 aaatgaccgg ctgctgatga gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt
     4981 aatgaaaatt ttaccgattt ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt
     5041 gccttcgcca cagtcgaaca accaaacttc attgcgctcg tctaaaagtt tcaatgctat
     5101 acttgtcaca tttctatgtt ttgcaggaac acctgcacct gttcctaaaa attgtaattc
     5161 cataatctac ctgactttct ttaagttcct ctttaatttt agggtaaaaa gaagggataa
     5221 tcaaccgtaa gtcgagaatg ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt
     5281 ctttgaaaat tactataaaa acagcagcga acccgtttga gatcctctgc tgttttttat
     5341 taatttgaag atccagaact ttcattgtcg ttggaagttt gcggatgttt gtccttccaa
     5401 gtcttttgtg cagcttcttt cgcatgattt tcagcttctt tcgcatcatg cacatctttt
     5461 tcgacatctt cataatccaa gcgagtaatt tctccgccta attccatcat taccaattga
     5521 ttcagtgggc gattgtcctc ttcatcagca atcaagatca gtcctgtagc tccttcaccg
     5581 attttatttc ctacaaagtc gaacacggtc tgggcatctt ttatttcttt ggcatcttgt
     5641 gtcgcaccga tcattcctcc ggcaaaccat cctaataaaa tccctagcgg accacctaat
     5701 acaccaacca acattccgat taatccaccc gtagaagatt tgttattacc ggtaaagtcg
     5761 ataaaatctt ctattttgaa ttgatgtgtc ccattaggat catggctgac aactgccatc
     5821 tgttctcctt taatcgaacg aacagcatgt aactttttga tttctgaaaa tgcctgatat
     5881 gcctggcttt ctacttgaaa atttaaaatg attactcgtt tgctcattaa aaacgcccct
     5941 ttcatacctt cttgttattt attttacctt taattgaaaa ataagaaaag aaaagcactc
     6001 tttttttcca ataaaatagg gctgtgacaa aagtcgtatc acagctcctt tttccgaata
     6061 aacggcgttc ttgaagctga tcacttctgt cacaacctcc cttaaatctg aatctatatt
     6121 ttttggtatt tgtttttatt tgcttgccta ttcgacaaat tcaaattcaa actcaccgat
     6181 acgtacaagg tcgccatctt tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc
     6241 acgtaattga cgagcaaaac gcatcactgt ttcatcatga tcgaaattcg tcatttggaa
     6301 taatttttcg atcttctcac cagataagat ccacgttgca tctgaatcac gatcgatttg
     6361 gaactcagga ccttctggat tgaatccata gtgtacagtt tcttcttcca gttcttcttc
     6421 gtacaatggg aattctggcg ttacttctaa caaatctgct gtagcattca ataacgcgtc
     6481 caatccttgt cgagtcactc cagaaattgg aaatactggg atgtcatcag caaattcatc
     6541 ttctttttcc ttgttcagct gttctttgaa tttcactaga ttttccggtg catccggcat
     6601 atccattttg tttgccacga tgatttgcgg acgctctagc aagcgtaagt tataagtaga
     6661 taattctttg ttgatggcaa gatagtcttc ataaggatct cgtccttcca tcccactcat
     6721 atcgatcaca tgtaaaatta ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag
     6781 cccaacacct tgcgaagctc cttcaatcaa gcctggcaag tctgctgcgg caaaacttct
     6841 gccatcactt gtagtaacca ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc
     6901 gatttttgga cgagcagatg aaataacgga tagtaaagtc gatttcccga cagatggaaa
     6961 acctacaagt ccaacgtctg ccaatacttt caattctagt tcgatttttc gctcttgtcc
     7021 tggttcaccg ttttcagcaa tttccggtgc aggattccga ggagaggcaa aacggatatt
     7081 ccctcgtcca ccgcgtccac cttttgccac aactagtgtt tgaccatttt caattaaatc
     7141 tcctaaaaga gcaccagtct ccgcatctcg gacagtggtt ccttgtggta ctttaacata
     7201 ggtatcttct gaaccacgac cgtgcatccc tttactcatc ccattttcac ctggctgcgc
     7261 tttgaaatgg cgattgaacc gaaaatccat caatgtacgt aacccttcat cgacgatcaa
     7321 gatgacatcg ccgccacgac caccgtctcc gccagccggt ccgccgtcag gaacgtattt
     7381 ttcgcgacga aatgcaacca ttccgtctcc gcccttgcca gctttcacat cgatcgttac
     7441 ttgatctaaa aacatggaca taattaagtc ctcctattat tcatttattt atttgactgc
     7501 ctgaatttcc acaataaaca aaagtcttac ttattttatc ggtgaaatct gattttgtct
     7561 atcatttcag caaaaagatt gtttaccttc ttcttttttt atcatttggt aaggtttcca
     7621 accttttttc tgctaccaag tataattttt tgacaaaata ttctttgcgt agcagaataa
     7681 cagtgtatta attgtattaa ttgaggatgc cgtttcacag cacccctaat aaaggaggaa
     7741 cttttgatat gacagtaaaa gtaggtatta acggatttgg gcgtattggc cgtctggctt
     7801 tccgccgcat caaagaagtc tcagatgata ttgaagtagt agcaatcaac gacttaacaa
     7861 gtccaactat gttagcacaa ttattgcaat ttgactctac tcatggtact tatcctggca
     7921 cagttactgc aacagaagac tcaatcgtag tggatggcga agcaactcgt gtttatgcag
     7981 aacccgacgc aagcaaaatc ccttgggcaa aagaaaacgg tgtagatatc gtactagaat
     8041 gtactggttt ttatacatct gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac
     8101 gagtagttat ttctgcccca gctggtgcta tgaaaaccat cgtctataac gtcaatgatg
     8161 atacattaga tgaaaacgac aaaatcatct cagcaggttc atgtacaaca aactgtttag
     8221 cacctatggc ttatttctta aataatgaat ttgggatcga agttggtaca atgacaacag
     8281 ttcatgccta cacttccaca caaatgctct tagatggacc tgtaaaaggc ggtaatttgc
     8341 gtgctgctcg ttcagctgct gacaatacta tcccacattc tactggtgct gcgaaagcaa
     8401 tcggcttagt tatccctgag ctacaaggaa aattacaagg acacgcacaa cgtgttccag
     8461 tcgttgatgg ttcattaact gaattggttt cagtattgaa aacaaaagta actgctgatc
     8521 aagtaaacga agcaatcaaa aaacacacag ttgacaatcc ttcattcggg tatgatgacc
     8581 gtcaaatcgt ttctagcgat gtcattggaa caactcaagg ttcgatcttt gacccaacgc
     8641 aaacagaagt aacaactgct ggtgatttcc aacttgttaa aactgttgca tggtatgaca
     8701 atgagtatgg atttacttgc caaatgattc gcttactaga aaaattcgct aacttgtaat
     8761 ttgatagatc aatcgataaa agaagccttt gactggatcc agtcaaaggc ttctttcttt
     8821 tcatccccta cttttaaact cgttcaatga acatattggc ttaaaaattg ttggactact
     8881 tgctgatatt tttcaggatc tgtcttgtat gcttccgcat gctcggctcc ttttacaaca
     8941 tacttttctt ttggtccctt cgttgcacga tagaccttat ctaacatttc aaatggtaca
     9001 aatgtatctt cgtctccatg tataaagagc attgggcgct ggtttttctt caattgttcc
     9061 actgcatttg cttcaccgaa aaagtagcca gcacggactt ttgtcactag actcgttact
     9121 tgcataagcg gaaatgctgg aagactaaac atatcgtcta gctgataagc aagctcatca
     9181 tgaacggaag aataaccgca atcttcaatg attgctttca cattatctgg tagtttttct
     9241 ccgctagtca tcataactgt tgctgctccc atgctgacac cgtatagaac gatttcttgc
     9301 gattttccat tgttttctag cactttgttt atccactgta catagtcttt acgctctggc
     9361 catccaaatc caatgtaatc cccttcgctt tttccatgtc ctcttgcatc aggaacaagg
     9421 acattgtaac ccatatcatg aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc
     9481 atatagccat gagccacaag agctgttttt tcacttttcg tttcagcagg aagataaatc
     9541 gctgataatt tcagaccgtc ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa
     9601 ctaaaccatt tatgtgcatc cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg
     9661 tctaaaaagt ctttctcaga aggcacaaca gcataattat aaaaataatt ccctgcaaac
     9721 actaacgcaa caatcagagc taaagcgacg cctaaaagga ttttcatcca aagtttcata
     9781 aatcatccat ctcatttctc ttttttcata taaccttgaa ttagtataat caaagaatct
     9841 ctactatgca atgttcattt ttataacccg cgaattgtaa aattaagtta gaaaaataaa
     9901 aaggcattta tggtacactc aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc
     9961 ataaatgacc tacaaacatc ttaccataga cgaactgaca atgatagaat catattatct
    10021 tcaacataat aagccggttg aaatcgctaa ccgaatggga cgtgctatac aaactattta
    10081 taatgtagtc aataagttca agcaaggcaa gactgctctt gattattggc accagtataa
    10141 agaaaataag aaaaaatgtg gtagaaaagt cattcaatta cctgctcatg aagtagatta
    10201 cattaaagag aaagtcactc ttggttggac gcctgacgtc attatcgggc gaaaagaaag
    10261 gtctgtttca tgcggtatga gaacacttta tcgtttattt tctaaaggaa tatttgatat
    10321 tgacacacta ccgatgaaag gtaaaagaaa acccaatggc catcaggaaa aacggggaaa
    10381 acaacagtat cagcgctcaa tccatgatcg acctgataat tatcctgatt tcaattctga
    10441 gtttggtcac cttgaaggtg atacgatcgt tggcattcat cataaaagtg ccgtcattac
    10501 tttagttgaa agattatcta aagtcattat cacgattaaa cccaacggcc gtaaggcatt
    10561 agatattgaa actgccctta atcaatggtt ttctcgcttc cctaaaaact tctttaaatc
    10621 tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta accaacatga
    10681 tattgatata tattttgcgg accctggaac accttctcaa cgcccattaa acgagaattc
    10741 taacgggatt ctgcgtcgta atggactgcc gaaatcaatg gattttagaa aagtgaatca
    10801 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
    10861 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
    10921 ttgacaaatc ataacctata aaaaaacaaa ataaaaaagc agaaataact ggctatttct
    10981 agcggattat ttctgcaata actaattatc tattttttat tttcaattaa tttaaagctt
    11041 gaccatggtt tcaaaaagtc aaggaggaat aattcgtcat cggagattct tcctacaaca
    11101 ttgacacgtc cgtcgttttt catatccttc aaggcgattt gtgtttctcc tttgtattga
    11161 ccgtaccctt cattgtcaat cagtacatcc ccatgatgca tatctcgcgt attatgaggc
    11221 gggaaatctt tatctttgta gtatacacga gtcatcgttg aacgaaggat atattctgag
    11281 cgatcccctc gataactatg aaggttatca aacaagcaga ttcgttcgtt ttctgtaata
    11341 ccttcttccg tatctacttt caatgttgga taatccgcgt taaatgcttc tgccatttct
    11401 ttcaattctt cttcacttgc gtacgcattt ccaatagaaa tatcatcaat caatcctgta
    11461 aggattaaat gcttcacttg tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac
    11521 aagccatctt gcgtaggcca agggccaaac tcagcagatt gcgaattgac gaatgccatc
    11581 gtattcaaat tatattttct gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa
    11641 ccagagtatc gatgaggata aaaattatga gaacctaata aattatccgt gtttggcgaa
    11701 taactcatga tgttgtcaac gtaattcgtg cccgaactca tattgatctc gattttgata
    11761 ccaaaaggat tgcgcgtcat ttttgcttct tctgctcctg tgaatccgat atctaagcgg
    11821 accccgtagg cacccatctt atggaaaaaa gataagtcat cgtaagaaat ctccaactgt
    11881 tcaaataacg ctggattgat atcaaccatt acttccatgc ctaggctatt cgcgtaatca
    11941 actacttctt taaattctga aagaactttt tctttatcat cattgatctg cagcaaactg
    12001 gtaaaaaccc gtttataccc atacttatga gctaaatcca agtacgcttt gtccttttca
    12061 aatgttgacc gttctggata aatcgagatt cctaattttc ccatattacc atttcctcct
    12121 gcatcttttc gtttttgtat ccacttacat tatagcaaac acaaagtaaa tggtctacac
    12181 cttttctttt tcttttaaaa taaatgataa attccaataa tttaagtagg tattttccct
    12241 ctattttgtt atcattaaag ataacaaaat agtaaattgg aaaggaacag ttatgaagaa
    12301 aaataaagtt attttaagta ttagtttagc agcagcattg ctgtttgctg ctggttgttc
    12361 tgcaaacaac acgacttctt ctagttcgtc tacagaaagt tcaaaaagta catctactgc
    12421 ggaaagcacg actgccagta gcgtcgattt gaacgcattg gaattaccac aattgacgaa
    12481 agatgtcgcc gaaaatgaag atctggtcga aatggtgacg aataaaggca caatcaaaat
    12541 caaattattt cccgaacacg caccaaaagc ggttgaaaac tttatgacac atgcaaaaga
    12601 cggttactac aacggactga ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg
    12661 cgatcctaaa ggcgatggca ctggagggga aagcatttgg ggagaaggat ttgaaacaga
    12721 gatcaacaac cacctttata atataagagg tgcgttatct atggctcgct cacaagatcc
    12781 taacagtaac ggcagccagt tctttatcgt tcaaaataca gatgatatgc atgatggtct
    12841 tttaaaagac gactatcctc aagcaatcat cgacgcttat aaaaacggcg gttatccttc
    12901 actagatggc cattatactg tcttcggcca agtgattgaa ggaatggatg tcgtggatgc
    12961 tattgcaaat atcgaaacag atagttcaga caaaccaaaa gaagaagtaa aaatcgataa
    13021 aatcaatata ctgcaagaag caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag
    13081 atgacaacgt atgctaggat tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc
    13141 ttttgttttg ctatatcagg ttttatagtc aagcattttg gttgacgatg aacgtgaact
    13201 cgcactcaca gactttttta tcatctacat atgctgcaga taacgctgta ccgactgatc
    13261 ccttcatttt ggtgatttca aaatgacatt tcagcacatc tcctggaaca actttttgac
    13321 ggaatttggc cttattgatc ccaccgatat aagctctttt tcctagaaac tcttctgatt
    13381 ttaggataag tatcgaacct gcttgtgcca gtgtttctag aatcaacgca cccggcatca
    13441 ctggattgtc aggaaagtga ccttggaaaa aatcttcatt gattgttaca ttttttgtcg
    13501 caacgatctt ttttcctggt tctaaagtat ctacataatc aatataacaa ataggataac
    13561 gattaggaat caattccatt acttcagtag cagataatac gtaagacata attaaactcc
    13621 ttctttcagg caattgccat tttcattact ttgactatca aagtatcata ttcctaaata
    13681 tttagcaatc tttttttaat ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa
    13741 taattaagaa atcaagaagt tattttagct attattctct atttttttac gactatcaaa
    13801 tttttatcag aaacaaagga gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat
    13861 tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca
    13921 aggggcagaa attatctata cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt
    13981 ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa
    14041 agcaatgaat gccatcggtg aatatgctgg ggagatccac ggccttgtcc atgcagttgc
    14101 ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat atttccagag acggctatca
    14161 attagctcaa gatatcagca gctactcttt aattgctgta tcaaaatacg cacaaccgtt
    14221 tcttgcacca aacagcggga tcgttagcat gacttatcta ggttcagaac gcgcaatccc
    14281 gaattataac atgatgggta ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc
    14341 agctgagttc tcgcctaaag gaattcgggt caatggaatc tcagctgggg caatcaaaac
    14401 attagctgtc acaggtgtta aagactatca aaaactgatc cagttatctg aatctcgtac
    14461 gcctgatcag caaggtgtga cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag
    14521 cccactttcc agcggaatca tcggtgatat tatctacgtt gacaaaggtg tccatctttc
    14581 ataaactttt gttgcattct ttaaagtaaa acatataaaa atcaggaaaa aactttttaa
    14641 agtctcttcc tgattttttc tttttacgtt tttttcaact cattactttg aaattattcc
    14701 acgacttcaa tccagccttc tggtgcttct atgtctccaa attgaatacc tgtcaattca
    14761 tcatacaacc gttttgttac tggtcctact tccgtttcac tataaaatac atgaagattg
    14821 ttattgtgat aaatcccacc gattggagaa ataacagccg ctgtaccaca tgcaccagct
    14881 tctgcaaatt gatctagatc atcaatgaaa atatctcctt caattgcttg cagtcctagt
    14941 cgttcttcag ctaactgcaa caaggaatat ttcgtgatac ttggtaagat agagggcgat
    15001 tgcggagtga tgaattttcc gtcttttgta attccaaaga aatttgctgc acctacttct
    15061 tcgattttcg tatgtgtggc tggatcaaga taaacacaat ctgagtaatc cagttctttt
    15121 gcttctgttc ctggaagtaa actcgctgcg taatttccgc cgacttttgc tgcaccagtc
    15181 cccttaggag cagcacgatc gtaatcagat acaataaaat tcgttggttt tagtccacct
    15241 ttgaaatacg ctcctactgg cgtacagaag acgacaaata aatattctgg agctggatgg
    15301 acaccaatgt tttcaccgac cccaatcagt aaagggcgca agtataatgt agcgcctgtt
    15361 ccatagggcg gcacaaaatc ttcatttgct tttaccactt gcttgacagc atcaacgaac
    15421 atcttttcag ggaccattgg catcagcaat cgtctagcac tttgattcat ccgtcgacta
    15481 ttttgttcag gtcgaaagag attgaccttg ccgtcttttc gacgataagc ttttagacct
    15541 tcaaaacatt gctgaccata atgaagcgct ggagagcctt catgaatcgt caggtaatta
    15601 tcttctgtca attctccttc ttcccattgt ccgtctttcc atttggcaat aaaacgccaa
    15661 ggagttttga tataagaaaa acctagattg ttccaatcga tttgcatata cttaccacct
    15721 tcgttatttt ctccacattt tatcattatc catattattt gtcattttat tttcagcttt
    15781 tttccttcat tatagttatt tttgctattt ccttttttga aaacttcttg gtacaataca
    15841 gaaagaatca aattttagaa aagaggattt tatgttttca ttattagcaa ctgccggtac
    15901 gacagactct tctggcgaga cattgacaga tcaagctgtc agcaaagtaa atgcgtttca
    15961 gcggttttgg gacgggattg attgggacgc cattttggct gcctttatcc aaaaaagttt
    16021 atatctgatt tttttgatcg tccttttttg gattcttcac cgtgtgggaa aatatctgat
    16081 tgataagtct tttaatcagt attctaaaaa agccattcta aacgagagcc gcttaaagac
    16141 tttacatagt cttttaaata ctatttttca ttataccctt ggtttctttt ttatctatgc
    16201 aattttatct gctatcggtg tgccgatcgg atcacttttg gctggtgccg gaattgcagg
    16261 tgtggcaatc ggtcttggtg cccaagggtt catgagtgat gttattaccg gattttttat
    16321 tatcatggaa caacagatgg atgttgggga ctacatcaag ctagcaaact taacgattga
    16381 aggaaccgtc gtatctgttg gtatccgtac ccttcagctg aaagcaacgg atggtactgt
    16441 ccatttcatc ccgaatcgaa acatcaccac gatcagcaat ctctctcgtg caaatatgca
    16501 agtactttta gatattcgga ttgttcctga agaaggatat gacaaaatct atgagatcat
    16561 tgatcgagta aatcgtacat tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc
    16621 tatctttgga ttagtggatc ttggaaacag caattttgcg attcgtacgg tctgctacgc
    16681 attgaacgga aaacaatatg ccttaaaaga agagttcttg agctcctatg tcaaagaatt
    16741 gacagcttct ggtttcacga ttccgaacag tccaattgct gtcggaaaat aatttggaca
    16801 aacgatgcaa cgaatctcac
//
LOCUS       DAYYNQ010000063        16714 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000063 DAYYNQ010000000
VERSION     DAYYNQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.063"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1462)
                     /locus_tag="ACHBJ1_001916"
     CDS             complement(251..1462)
                     /locus_tag="ACHBJ1_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430844.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1581..2216)
                     /locus_tag="ACHBJ1_001917"
     CDS             complement(1581..2216)
                     /locus_tag="ACHBJ1_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFM9430845.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2352..3005)
                     /locus_tag="ACHBJ1_001918"
     CDS             complement(2352..3005)
                     /locus_tag="ACHBJ1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430846.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3108..3314)
                     /locus_tag="ACHBJ1_001919"
     CDS             complement(3108..3314)
                     /locus_tag="ACHBJ1_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFM9430847.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3390..4136)
                     /locus_tag="ACHBJ1_001920"
     CDS             complement(3390..4136)
                     /locus_tag="ACHBJ1_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFM9430848.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4296..4520
                     /locus_tag="ACHBJ1_001921"
     CDS             4296..4520
                     /locus_tag="ACHBJ1_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HFM9430849.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4544..5305
                     /locus_tag="ACHBJ1_001922"
     CDS             4544..5305
                     /locus_tag="ACHBJ1_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM9430850.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5317..5574
                     /locus_tag="ACHBJ1_001923"
     CDS             5317..5574
                     /locus_tag="ACHBJ1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430851.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5666..5917
                     /locus_tag="ACHBJ1_001924"
     CDS             5666..5917
                     /locus_tag="ACHBJ1_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HFM9430852.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5917..6066
                     /locus_tag="ACHBJ1_001925"
     CDS             5917..6066
                     /locus_tag="ACHBJ1_001925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430853.1"
                     /translation="MFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDEAEYRAKKP
                     TEGAD"
     gene            complement(6180..6485)
                     /locus_tag="ACHBJ1_001926"
     CDS             complement(6180..6485)
                     /locus_tag="ACHBJ1_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430854.1"
                     /translation="MKDKEIELQKHDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAV
                     KASGYAVLPYIQNEDIEVMKIFLSRFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6748..6972
                     /locus_tag="ACHBJ1_001927"
     CDS             6748..6972
                     /locus_tag="ACHBJ1_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430855.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6982..7176
                     /locus_tag="ACHBJ1_001928"
     CDS             6982..7176
                     /locus_tag="ACHBJ1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430856.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7179..8201
                     /locus_tag="ACHBJ1_001929"
     CDS             7179..8201
                     /locus_tag="ACHBJ1_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM9430857.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8164..8979
                     /locus_tag="ACHBJ1_001930"
     CDS             8164..8979
                     /locus_tag="ACHBJ1_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFM9430858.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8997..9779
                     /locus_tag="ACHBJ1_001931"
     CDS             8997..9779
                     /locus_tag="ACHBJ1_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430859.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9779..10627
                     /locus_tag="ACHBJ1_001932"
     CDS             9779..10627
                     /locus_tag="ACHBJ1_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9430860.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10624..10866
                     /locus_tag="ACHBJ1_001933"
     CDS             10624..10866
                     /locus_tag="ACHBJ1_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430861.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11009..11377
                     /locus_tag="ACHBJ1_001934"
     CDS             11009..11377
                     /locus_tag="ACHBJ1_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFM9430862.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11392..11553
                     /locus_tag="ACHBJ1_001935"
     CDS             11392..11553
                     /locus_tag="ACHBJ1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9430863.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11550..11876
                     /locus_tag="ACHBJ1_001936"
     CDS             11550..11876
                     /locus_tag="ACHBJ1_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430864.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11873..12688
                     /locus_tag="ACHBJ1_001937"
     CDS             11873..12688
                     /locus_tag="ACHBJ1_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFM9430865.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12690..12815
                     /locus_tag="ACHBJ1_001938"
     CDS             12690..12815
                     /locus_tag="ACHBJ1_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430866.1"
                     /translation="MGFFMAKILLFLGLVGAAYLVYALLSQTDDKEDDNNDEMKF"
     gene            12827..13138
                     /locus_tag="ACHBJ1_001939"
     CDS             12827..13138
                     /locus_tag="ACHBJ1_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM9430867.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13135..13380
                     /locus_tag="ACHBJ1_001940"
     CDS             13135..13380
                     /locus_tag="ACHBJ1_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430868.1"
                     /translation="MIKKLIQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13377..13793
                     /locus_tag="ACHBJ1_001941"
     CDS             13377..13793
                     /locus_tag="ACHBJ1_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFM9430869.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13790..14128
                     /locus_tag="ACHBJ1_001942"
     CDS             13790..14128
                     /locus_tag="ACHBJ1_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430870.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14125..14355
                     /locus_tag="ACHBJ1_001943"
     CDS             14125..14355
                     /locus_tag="ACHBJ1_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430871.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14362..14565
                     /locus_tag="ACHBJ1_001944"
     CDS             14362..14565
                     /locus_tag="ACHBJ1_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM9430872.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14562..14858
                     /locus_tag="ACHBJ1_001945"
     CDS             14562..14858
                     /locus_tag="ACHBJ1_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430873.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14935..15348
                     /locus_tag="ACHBJ1_001946"
     CDS             14935..15348
                     /locus_tag="ACHBJ1_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM9430874.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15361..15747
                     /locus_tag="ACHBJ1_001947"
     CDS             15361..15747
                     /locus_tag="ACHBJ1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430875.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16298..16525
                     /locus_tag="ACHBJ1_001948"
     CDS             16298..16525
                     /locus_tag="ACHBJ1_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430876.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16522..>16714
                     /locus_tag="ACHBJ1_001949"
     CDS             16522..>16714
                     /locus_tag="ACHBJ1_001949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430877.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5664 a         2726 c         3485 g         4839 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtt
      121 cgttatttaa tggtatttta aatgaagata agcgaattta cagcattttt aaatgcttat
      181 ataccgcaaa ttatcccaag tttttgtata tcctacccga gtttataccc gaaaatataa
      241 ttttttagtg ttattttata tcttttttta ataggttttc aaacgctgta gttgctgtat
      301 ctttttcttt gcgtaagaca tggccataaa cattcattgt agtattaaaa tctcggtgtc
      361 ccaatcgttt ttgaagctct tttggagtca tattaggatc gctaagcaaa taggtagctg
      421 atgtatgtct aatgtcgtga aaacgaatgt gacgcaaatt gtgacgtttg gtgaatcgtt
      481 tgaacttctt atacagataa cttccacgtg gcaaagttcc atcttgtttt gtatcaaata
      541 taaaatagtg ttttaatttc tttatattta atttccaacg aatcttttta ttctctttga
      601 ttaattcatc taacatatca aatagataag caggaccggt tactattttt tcatcatcat
      661 catttttcaa acctggcagc aaccgaatat ctgattttcc atccacttct gaaatccgct
      721 gatgaaatct tatttcttgt ttgtcaaagt caatatcttt ttcttctaaa gctgcaattt
      781 ctccttgtct tgctcctgta ataaatgcta gaacaattaa agttttaatt tctatgcttt
      841 cctcaaatgc tgcttttagc attgcatgaa tttcttcttc cgaataaggt tcttcgatat
      901 tcttttttac gcctttttct tttggtattt ttacattttc cacaggattt tctttaattg
      961 ctccgtactc atttttagcc atcaaaaata agttgttaac ggcgtataac atacgaagct
     1021 ttgtatttct tgataaaggt ttatcagagt ttctcccagg atcttttgta ttgattcttt
     1081 gagcattaac aacaattttc tttatcatgt atggtttgat atcgattaga gggataaaat
     1141 caaatttatc aagaaatcta ttctcgatca ttcgcttata gttatgataa gactttggct
     1201 ctaaattttt ttcggcctct gaaagccata tttttctata aaattcacca aaagttatat
     1261 tgaacaaatc caacgaatca tcggtcattc cttcgaattg ctctaaccag atgttcaatt
     1321 ctttatatgc cgctctttta ctacttgctt ttatagtttt gctttttctt tttgccgagc
     1381 catcaggttt gtatcctaat acagctctta gtctatatgt ttcgttgtct acttgttcta
     1441 agtatcctgt tacttctgac ataattgaat cagctctctt tctctgatac aataggtact
     1501 ataaagaagc ctatcgtata ggtttgtttc tttcttagaa cacgctcgct ttggtcggtg
     1561 gggcgtgttt tttatactaa ttagttgtga agagcaattg aaactgtctc tgttccaaag
     1621 taacctgttt gtgcttgcaa tacattattt tttgcatttg cttgggcttc agagatatca
     1681 aaaactattt taccagtttg ttgcatatca ggatttaagc tttccataaa gaaagagtta
     1741 gtgatattac cattttcatc ttgatttgcc gacatagatg cagccgaatc agcttcgaaa
     1801 gtttttccat ctgcttttaa tttgaagaat gagctatcaa cagtcacagc cttatcacct
     1861 gcatttttta cagataagtc aactactaag aaagtgtctt ttgcgttagt aggaagtaca
     1921 gatgggccaa cttgttttac tacttcaacg gagtttactt tgtattccat tttccctaca
     1981 gcaacgtcat caccgatttt ataagtcttt tcttcttttg ttgtagtttc ttttgtagtt
     2041 tctttattag tacttgttga tgttgcagta gctgaatctt tgttttcacc gccacttaga
     2101 gcaccaccaa taccaaaaaa caaaagtact attaataccc aaaaccatac ccgtttataa
     2161 aacggttttt taaccttata cattttccca tcttgaccca taactttttt tgccatttaa
     2221 atattcctca tttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg
     2281 ttgcagcacg ggctttttta tttataactt ttttagagat tataggcaaa atagtagggc
     2341 ataaaaatat attattgaat tccgtattta aaaaatccta attgaacttc accggaagtc
     2401 ttttgctgtg ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca
     2461 acaggcgaca tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc
     2521 tgagcgcttg tttgttggtc ttctggattt tgttgagatg ctgcttgttg ttctttctga
     2581 tcttgactga taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg
     2641 ccgaaagctt cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa
     2701 gtgcgttgtt tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga
     2761 ctggagctta taactgttgg gctaggttcc gccgtaactt ggttggagct tgtaactgtt
     2821 gaactagaat ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct
     2881 tttgttttac ttatttttgt ttcttggtta gaagtggcat ctgttgattc agctttttta
     2941 ttatttgaac aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt
     3001 ttcattttat actcctcttt tctttgatac aataagttgt aaagaagcct actataggtt
     3061 taacatccca cgtccacaaa ctttggtcgg tgggcgtgtt tttttgttta tagactttcg
     3121 aaaactaaag tagcttggat tctatcgcca ccaccgaaac ctttactacc accgtttgta
     3181 gtggaaatag tgtgtaatcg ataaccttta gaacactgtc tatttatagt gttttcaagt
     3241 tctgataggt tttgcgaccc ttttcctata aacttttctt ttaatacaac ttgcaataca
     3301 acgtaacttg gcatattctt ttccttcttt ctactatgat aataaatact aacccctagc
     3361 cggagcgcca actacgactg gggtattttt tagaatacga ctttcccgat tattctaatc
     3421 tgttccggtg agacgactct atcttcatat aattcgttta tagagcgtag aataatcgtt
     3481 tgactctcat aatccttata tacttcttta cacgtcacac cgtcaccatc tatttcaacg
     3541 atagcaatct caccgtcttc cacgtctggc tgttccttaa caaatacaaa ttgtccattc
     3601 ctgataactg gttccattga atcgcctata ataggaacag caaaatcagc attgtttgga
     3661 actctttcaa attcttcttc ctctacaact gtatcgccat aaactaattc agtcgggttg
     3721 gcggcagaag ctgaattttt gattgtcgga agtttttcct ttttaggaaa gtgcaccacg
     3781 tttcctcgat tttgctcttc gagttgctgt tcagcaaagt tgtagacttt tgtttgtctt
     3841 ggtttttcta attgattgta gattgtttca atggatggaa tgttattttt cttatcttct
     3901 ttaggaacat cgtaaccaag caaccatgcc tcactcacat ttaaagtttt agccaacaaa
     3961 taaagcttgt gttggtcggg cgatgactta ccattgacat attgcgatag atgacttttt
     4021 gacattttta ttccagtttc tttttgcaaa ggtaaagaca tattcaaaat atcaacttgc
     4081 tttaaatttc tttcagacat taactgatta agtcgttgct gagtcgtttg cttcatctta
     4141 tacctccttt ctgatactta ttatatacct tattgaacat aagttcaata aaaaagttaa
     4201 attaatttga acttttatgt tgacaatgtt ttatgcagga gttatacttt ggttacaaag
     4261 ttcaaaacat ttgaactaga aaggagcgaa ctaatatgag ttttgattat tctaagttgt
     4321 cgggaagaat tgttgagaaa tatggaacgc aatacaattt ttcaatcgct ttaggtttgt
     4381 cggaaagaag tttgtcatta aagttgaata accgagtacc atggaaaagc accgaacttc
     4441 aaaaagccat tgaattgcta gatattccaa aagaagaaat tggcgaatat ttttttaatt
     4501 tagaagttca aaatgtctga acttaagaaa ggaattcgaa aaaatgaaca cacaaaaatt
     4561 tgaaaacaat ctatttgaat tagaagttaa gacggaaaat ggtgaatcgt tatttaatgc
     4621 tgaaacagta gcaagaagtt tgggtttcgt ggaaacaaaa aacggtaaag aatatgttcg
     4681 gtggagaact gtcaatggat atttaaaaaa atacctttcg caagaagttg cgaaaaacga
     4741 ctttatcagt gagccaatgg tttacaaatt agcctttaaa gcaaacaacg tacttgctga
     4801 aaaattccaa gactggttag caacggaagt cctacctcaa atccgaaaac acggcatgta
     4861 cgcaacagat gaactactta acaatccaga cttgctgatt gaagtagcaa ccaaactcaa
     4921 agaagaacgc acgttacgat tgatagctga acaacgagta aatgaattac aaccgaaggc
     4981 agactattac gaccgaatct tgaacaacaa aggattagtt acagttagta cgattgctaa
     5041 gaactacggt atgagcgctg tatcgtttaa taaattgcta catgaattag gtatccagtt
     5101 caatcaaagt ggcacatggc tgctatacag taagttccaa gacaaaggat acacgcatat
     5161 tgagccattt gattatgaag ataaaaacgg caatcggcaa gtaaaaacaa ggatgaaatg
     5221 gacgcaaaaa ggacacatct tcttatatga aacattgaag aagaataatt acttaccgat
     5281 gattgaacgt gaacaaacag cataggaggt aacactatga acgaggaaat gagccaaaca
     5341 atcaaggtac aaaaaatgat agatgatcta acacatgcaa ttgatagcca agccgataaa
     5401 atcatcaagg aattgcaagg acaaaaagtt tgggatgcaa aaatgctttt caagactatc
     5461 aaatttgaaa tgaatccaac aaaaagaaag ctcgccgatg tgttggaaga aaagttggct
     5521 tccgctatca acgagcaaga attactattt gaaaccgata cttttaacag ctgatgtttt
     5581 atagatgaca gtttcatccg aatctatatc attatagatt cgattattgc aatgtacgaa
     5641 ttataaatac aggaggaaat aaccaatgga gcaactagct ttagttaacc tctcagacct
     5701 taaagcttct ttggcagaat ctgagattgc aaatgaagtg tgggatacaa agcaagctgc
     5761 ggaatattta aaaaccacaa cacgcacgct gactaaagat gccgaatctg gaaagattcc
     5821 agcagcgaaa gttggcagag agtggaggtt tagtagtatc gctttgtatc agtatctaaa
     5881 aggaggaaaa acatgggtaa attcaacaga gcactagtgt tcagcgcacc cctaatcgtc
     5941 tatgctttag gactttgggg aagcagacaa gcattaatcg gcacgatcgt ttacatggtt
     6001 tggattttta tggggcttga tgaagctgag tacagagcga aaaagcccac cgagggggct
     6061 gactaaagtg tggttctgtt tattaggtgt ttatctcgtg gccgttttag taaataacta
     6121 catgagaaaa cgtggcgaat actggtatac atcctatgcg gttctagtat gtctaatgct
     6181 tacaattttt ctaatgattt attcaaaata ggtaatactt tgtcaatcaa ataagtttca
     6241 agagattttt gttctgcatc agtgttgccg taaccaaaac ggcttaagaa aattttcata
     6301 acctctatat cttcattttg aatatatggc aggactgcat aacctgatgc ttttacagca
     6361 gatatatttt tatcagtgtt actaccaata caaataccca cattgttgag aaatgttgag
     6421 aaagttgttt gtattgtttg ggtttttgaa tcgtgttttt gtaattcaat ttctttatcc
     6481 ttcatcgatt caacatgctt attatttata accgctgtaa tcgaagggga tataagagct
     6541 actaaagcta ggataatcga aattgtgatc gtgttatcga aactcatttt ttcaccatcc
     6601 agtttttaaa ccattatatc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg
     6661 aaaaataaaa aaagacacga acgccagcaa gcatgatcgt gtccaaaaga aaaatctttt
     6721 aaaccattat aacacaggag gaaataaatg atctcagtta aagggttagg cgatgaaata
     6781 ttcgaagcaa tgatgagcaa agcacaacag gacatcaaag aaaaaatcat ttctgctgca
     6841 gcgcaaggac aaactagctg cactgttcga tcgagaggaa cgacaccaag ttttcttttg
     6901 gcgctagaag atgaaggcat tttgaatatc aaacgaagag atggcagcgt cagattgttt
     6961 tggcaatttt aggaggccgt gatgaaagat tttgattctt taggcgccag acagcaacca
     7021 ccaaatgaag ctagtcctgt tggagttgat tggcaagaaa atccgttata tccaggtgat
     7081 acttgctatc taacagaaga aggctatgta ccagtagacg caattctaga gtacgtccag
     7141 caacattatc caaagattga attaggagga atttaggaat ggcaaatgat ttaacacaaa
     7201 caacacaacg ttcattagat gagcaggtaa tcagtaatct tggacggtta caagaacaag
     7261 gtttagaaat gccaccagga tatagtccac aaaatgcgtt aaaaagtgct tttttcgaac
     7321 tcactaacaa tacaggagga aacttgttgc agatggctgc aaacaatcaa gaaatgaaaa
     7381 cctccatttc taatgcactt ttagacatgg ttattcaagg gttatctcca gcaaaaaaac
     7441 aatgctattt cattaaatac ggaaacaagg ttcagctaat gcgttcatat tttggaacaa
     7501 tggcggtcct tgatcgtgta actggagggg cagatattac gcctgtagtc gttcgacaag
     7561 gtgatgagtt tgaagttgca atggatggac caaacatggt tgtcaaaaag catgaaacaa
     7621 aattcgagaa tttagacaat gaaatcattg cagcctatgt agtcatcaag ttaacaaatg
     7681 gtaaagagac cacaacagtc atgacgaaaa agcaaatcga tcaaagttgg gctaagtcaa
     7741 aaatgaaaga ttctggaccg caaaaggaat ttccagaaga aatggctaaa cggacagtaa
     7801 tcaatcgggc agctaaaaca ttgatcaaca caagcaacga caacgattta ttagttcaag
     7861 ctgcaaaaga tacgttagaa aatgaatttg acaatgatcg gaaagatata acgccacaaa
     7921 cagaaaaagt ggctactctc gaacaaaaat tcttttcaaa caaaaagatt actgaaccaa
     7981 tacaaaaaga accagatccg attgtgattc cagatgatat ccaaaatgaa gtaactcgag
     8041 ttgctgatgt acctgcccat ccagaaatag agcaagcaca tttgatcgaa aatgaggata
     8101 tcaatccaat tcaagaagaa ttgatagata ttccagactt tggacgcgag gaaggtgtag
     8161 aagatgtctc agaatttgaa gacgacgagt accctttctg atgagaatta ttactccaat
     8221 gaagctgact ggcagtacat gtcaacatca caatataagt cctttttaaa atgtgaagcc
     8281 gcagccttag caaagttgaa aggcgattgg gcaccgacgt cggatcccaa agccttactc
     8341 gtaggaaact acgtacattc ttattttgaa tcaaaagaag tacatgaagc gttcaaagaa
     8401 gaaaataaat ccaaaatgtt ttctagtagg aagccgtatg gattgttgaa agatttccaa
     8461 attgctgaac aaatgattga acgacttaag caagaagaag cattcatgaa catttatcaa
     8521 ggtgagaaag aaacgatcgt gactggggaa ctatttggaa cgacatggaa aggcaagatt
     8581 gattgcttga atgttgagga tgagtatttt gttgatatca aaacaaccaa agacatgcat
     8641 gagcggaaat ggaacgaaaa ctacggatca agagaaacgt tcattgtcaa ttttggttac
     8701 gtacttcaaa tggcaatcta tcaagagctt cttttacagc aatacggaaa gatgttcatg
     8761 ccaatcatcg ctgcggtatc gaagcaaacg cctagtgagg cgaagttgat aacgattgat
     8821 caagataaga tgagctacga gctaatcatg ctaaaagaaa atattgaaag aattgtcaaa
     8881 gtgaaaaatg gcgaagaaag gccgatacct tgcggtacgt gcgagtattg tcgaggtaac
     8941 ctttcaatta ctggattcac tagcatggac gatctttaaa aggaggtgct ttgcgaatga
     9001 atctaggata catcaagtta taccgtaaag tgactagttc attcgtttgg accaattccg
     9061 atatgttcaa gctatggata ttgtgtttaa tgaaggctag tcatgaagat agaaaatttt
     9121 tatttaacgg tcaagaagta cgtttgacaa gcggacaatt cgtcacagga gcccatgcaa
     9181 tagcaaaaga gtacaacgaa ggagtcccga gtgacaaagc aattgcatgg cgaacgctat
     9241 ggagatggct taagaaattt gaaaatgaag aattattgac aatccaatcg aacgctagat
     9301 atagcgttat aacaataata aattggtctg actatcaatc gagtgacaag ccagtgacaa
     9361 gcgacggaca atcgagtgac aagccagtga caacaaacaa gaatgataag aatgataaga
     9421 atgataagaa taataatcct cgcaactctc gaaaaacacg agagtatgca gatgacgatc
     9481 caaataaaaa attggccatt cttttattaa aactcattcg aaagaatcgt agtctcaaag
     9541 aacccgattt gaacaaatgg gccaatacga ttcgtttgac gattgaagct gacaaacgaa
     9601 ctggaaaaga agttcaagac atgattgttt gggctactaa ccatgatttt tggtccacag
     9661 tcatttcatc gccaactagc ttaagaaaaa atttcgatac aatggtgggt caaaaaaata
     9721 aaagaaagca acaaaatatt tctaatgatg agttaccaga aacaggtgag gattggtaat
     9781 gaataaaaaa ctaagtgcaa tggctgcacc ttacggcgga ttaaaaacag cagatcataa
     9841 ttgcccgaaa tgtggtgatc cattgtatat ctggaaaaca aaaaataaag atggtactga
     9901 tcgatgcggt cctacatgta tcaataagat ttgtggttat cgagaaatgg taactaaaaa
     9961 tcaaaaagaa gctatccaaa aagcgaatga agcaatgaaa aaggacgcta tcaatcgaat
    10021 gcttaacagt tcaatgatta cagatgatgc catatggacc ttcgattttg atggatacaa
    10081 agtagttgat caggaaacag cacaaataaa agcgatggct caagaatggg ctaaaaaaat
    10141 tgtaaatggt agcacgattc atgcagtgat aaccggtaga acaggagccg gcaaaaccca
    10201 tctaggcgct gcagtgatta aagaagtcat gagggcatct aattataaaa ttgcttgttc
    10261 atttatcagt tatcgagaac ttttagaaca gttgaaattt gcaatgaatg atccagaagc
    10321 aagaaaagcc gtaacaggtt cgctgatggc tgagataaag aaatcagatt tcgtggtaat
    10381 cgacgatttg ggtgcagaac taggaagaat ggaagaaaac aaccaggcaa cgccttatga
    10441 tgttgatgtt ctcacatcgc tcacagaagc tcgtctaaac aaagctacga tattcacaac
    10501 caatttatca tcgaagcaat taaaacacgc gtacggtgag cgagtgttct ctcgtgtaat
    10561 gaatggaacg aaagggaaca tagctgtatt caaaacaacg acagacaaaa ggaggaatcc
    10621 agtttgacct ttgtagtgaa aaaaatgtgc tacttagata gccgagggcg aggagaagct
    10681 agtgtcgagt ttgctaaaca ccacacgact aaagaagagg ctgaccttgt tgcaagtgtt
    10741 tgtggcggcg aagtagtcga agttattaaa cccaaaagac gattttcaaa acctaagaca
    10801 aagccagtga aaaaagagtg ttgttgtccg aaaagtaacc aagcatggat gagaggtgca
    10861 aaatgacttg tttaaaatgc aacgatgaaa cagttatttg gtataagaca ttgcttggat
    10921 ggtcaacttg tgaaccttgt ccaatatgca atgaaaatgg acgacgttcg aaagaacgac
    10981 tcgaaagact aaagaaggag tatagcaaat ggcaacaaga agcaaatacg gaaacaaaaa
    11041 gcacgaagta gacggcatca cttttgattc tatcacggaa gcagagtatt acgatctagc
    11101 cttgtggcaa gcacaggcga atggttggaa agtgaagctc caggaaaggt ttgagctgat
    11161 gccgaagttt gaactagagg gcaagaagta tcgcaagatc gagtatattc ccgacttcac
    11221 attttacaaa agcggcaagc tggtcaaagt cgtagatgtt aaggggatgc agacaaaaga
    11281 ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa gtgccgttga tattagccaa
    11341 aaaatatcgg aatacgttca aggaagagcg tttttaacga ggtggtccat catgacaaca
    11401 gaagaagtga ttcaaatgcg aattcgaagc cttcggcgtg agattgacga tctggaacga
    11461 acaaaggcag tgatggtcaa tgaaacggct agaaaggcaa tcgatttgca catagagaat
    11521 ttaagaaggg aaatccgtag attggaggaa tgagcgtgga taaggaagcg gcttggcgaa
    11581 aattaatgtt gctgattcaa gatgagaact ggcaagaaga tgaagcagtg gttgctgaag
    11641 ttcagcgtct aggaaagatt gctaacggac gtatacgaaa aaagccagac aaaagaaaac
    11701 agcgcaaagg gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag
    11761 ctagtgagct gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc
    11821 gaaatcacac cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa aaatgaacga
    11881 aaacaaatta atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg
    11941 aggatttaag ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa
    12001 cggcggtatc aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt
    12061 gatccaatat ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag
    12121 cgacggcaaa aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc
    12181 agcaaaaatg tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt
    12241 aaaatctaag cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt
    12301 cctttcagga gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt
    12361 tgaatctaaa gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga
    12421 aacacaaaaa tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct
    12481 agacgcagaa actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc
    12541 agaagcagag gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg
    12601 ttatgaagaa gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac
    12661 tgtagttacg gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt
    12721 tcttaggttt agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca
    12781 aagaagatga caacaacgat gaaatgaaat tttaggagga gaaataatgg acgaactaat
    12841 cacaaaagta gagcagtggg ctaaagacaa gggactggat caagcagatc caaaagcaca
    12901 gtttttgaaa gtagctgagg aattcgggga aatagcttcg gcgatggcaa gaagtaatga
    12961 tgaactattt aaagatagcg taggagacgt tatcgtcacg ctgattatcc tttccatgca
    13021 aaaagggaca aacgtacaag agtgtttaga aatggcatac aacgaaatca aaggacgcac
    13081 agggaaaatg gtagatggtg tattcgtgaa gtcgagtgat ttggagggca gcaaatgata
    13141 aaaaagctca ttcaattcag catggattta tatgatatcg aatcaggagc aacactatct
    13201 gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat tttgtgggta
    13261 gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa agcggttgag
    13321 tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga cagcgaatga
    13381 taccgaagtt tagagcgtgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc
    13441 attttactaa aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga
    13501 aatcatatca cgtgcgaaac atcgacctca tgcaatccac aggaatgaaa gataagaatg
    13561 gtgtggatat atttgaaggg gatatagtat tagtcagcgt gcgaaatggc ttcgattact
    13621 tagataataa agtttgtatt gtcaaaaatt caatagatta ttccggatta gtttgtgcca
    13681 ctgttgatga agacttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt
    13741 atgaagtcat cggaaatata tacgagaata gcgagttatt ggaggtagag tgattatctt
    13801 agctactgac tacatcgatt ctttaaaaga tgaaaatggg aatgtcccag cattgctaaa
    13861 ttgtggaatt aaaagcaaga atagaatcat tttgttacgg atatcggctg atttagcaaa
    13921 atatctttat ctgagcaaga cagcagtata ttacactgcg gctacacgtt atcagtacaa
    13981 gggaaaaaca atcagtcagg attattatga tcgtttaatc agtcctgata tgtatgggaa
    14041 ttcagaatca gcaattaagc tgtttggcgc aatagagata tatgccgatg attttccaaa
    14101 tttatggttg aaggaggaag cggaatgaaa tacgaaatac cactaagtga agcagacgtc
    14161 cagtcaatta ttaacggtcg ggaggttaat aaaaaacttc ctgatggtac tgaattagtc
    14221 atcagacaaa gttatttgaa agatatggca gctccagtat taattgatcg ttttaacgtg
    14281 actgattctg tggtagagaa ccacttgaaa gaatttcgat caagtataaa cgacactttc
    14341 agattaggga gttgattgac aatgaacacc agacaccgca gagtagcaaa actaagaaaa
    14401 caggaactga atgtactaaa gacaaagttt gaaaaagaat atggaatttc agcagaagaa
    14461 acatataaag tggcaagtca gtgtgttgct gatgcaagtg atgctattcg taagtttgga
    14521 atttcgatat taagtgatga tcgtaaatgg gaggaaaaag aatgaaacta aaagacggat
    14581 tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca aagaatccta
    14641 aaacacgtaa gaaaccaaaa gtcaaagtcg gtgacatggt ccgctgtgaa gcagaggagt
    14701 tcatttatcc atttcgtgga tatgtagaac acgtctataa tcactcagcg atcattcgca
    14761 ttgaaaacac gatggaatgt gataaatgga cagctaaaag caaagagaat ttagcagtag
    14821 tgagattggt ggatatggaa ctaatcaatg acaaataaaa aagccggatc gctccgacta
    14881 acataataaa acagacaagt ttattatatc acataaaagg agcagtttga attgatgcaa
    14941 ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt gctgaagaac
    15001 tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc gccgattatt
    15061 acggatatgc cgaaggcacc gaagcacggc aataaggcag aggatgcatt gattcagatg
    15121 atggatatag aagcggagag agacgcgatt ttagcggctt tgatggctct tagtctgatt
    15181 agtcgtcaga tactctacta cagtttttgt gtgccagaca gcttctcaaa ctacagaatt
    15241 agccgtgaag tgggttattc agaaagaagt atacaacgga tgaaatcgga agctctaata
    15301 gagtttgcag aagcatataa acacggaaga ataattgctt ataaataggg ggaccatata
    15361 atgtggaaag attatgtatc gttgaaagag ttgaaaaaag atcttgtttt caaaagaatt
    15421 gtagaatggt cagagagtga attaatttta gaagatggaa ccaaaatgga agttgtatgt
    15481 agcgaatcag attgctgcgc atgggccgaa ggtgaattca aaaatgtaaa gcttgatgca
    15541 gtgattacag atataaagat ttttgataaa ggtaaccgtc tctataatgg tgacggacat
    15601 acctcttatg ctgaagtcgt tgtttatcat aatcgtaatg agatcgctaa agcagaatgc
    15661 acagcgaatg atgggaatgg cggctactat tatagtgttt gtgctttaaa ggtcaaagac
    15721 aaactttgta tagtgactga cgcataaaaa aatggcggtt ttttggcgga atgatggcgg
    15781 tttttagcca tttatcagtg atattatggt agtgtcgaaa gattagtgat aggtctgaga
    15841 caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc ttcgatagac
    15901 agcaacggaa atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt
    15961 atctatcatc cgttgctgtt tattgttaca tacagatcgc ttaggcggtc tttttatttt
    16021 ggccgaaaac ctacattttc gatggccgat tattggagga ggaatagcat gttcaaacta
    16081 tctgaaatca ttaagaagtc cgacgttgag aaattagaga tgttaaaaaa gaagttgaag
    16141 aaatagcagt ctcttcgtga ggctgtttta ttttgctcac aaaaatagac cactatcggg
    16201 taatagtggt caggaattaa atgaaaaaga tgttagggtt gttagctaag tataacatca
    16261 taacgcttac aaaacaatgc aagaaggata aattattatg agaacttact ggtatgtgtc
    16321 attaaacaat aaatatccga agccaatgaa aggacagcat aggcgtgtag tcatgtctgt
    16381 tcaaatgaag gcgaagtatt cgattgtaga aatgctaaga gaagctacac caaaagagat
    16441 tgatcagtat aagttggttc ttattggcat tggttggttt aaggatgaac atattcaaac
    16501 aaacatgaag aggtggttga gatgaagtta tattatgttg aaaaagcaaa ttgttgggtg
    16561 gttgaaggct gtctgtcaaa gtacgctaca gatattatat ctactcaatt ttcagagttg
    16621 ggattgaagg ttatcttttg tggtgagttt gtcagtgtga aagaagcggt attgccaatg
    16681 acagcaaaga gcagacgctc gcgtggttca agtt
//
LOCUS       DAYYNQ010000064        16303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000064 DAYYNQ010000000
VERSION     DAYYNQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.064"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1580
                     /locus_tag="ACHBJ1_001950"
     CDS             153..1580
                     /locus_tag="ACHBJ1_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430878.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1672..2037)
                     /locus_tag="ACHBJ1_001951"
     CDS             complement(1672..2037)
                     /locus_tag="ACHBJ1_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9430879.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2034..2399)
                     /locus_tag="ACHBJ1_001952"
     CDS             complement(2034..2399)
                     /locus_tag="ACHBJ1_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM9430880.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2591..2839)
                     /locus_tag="ACHBJ1_001953"
     CDS             complement(2591..2839)
                     /locus_tag="ACHBJ1_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430881.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2838..2966
                     /locus_tag="ACHBJ1_001954"
     CDS             2838..2966
                     /locus_tag="ACHBJ1_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM9430882.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3023..4717
                     /locus_tag="ACHBJ1_001955"
     CDS             3023..4717
                     /locus_tag="ACHBJ1_001955"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM9430883.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4790..4990
                     /locus_tag="ACHBJ1_001956"
     CDS             4790..4990
                     /locus_tag="ACHBJ1_001956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430884.1"
                     /translation="MIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTW
                     LDLLPQMQAFEHRLPRGYEKGV"
     gene            5368..6501
                     /locus_tag="ACHBJ1_001957"
     CDS             5368..6501
                     /locus_tag="ACHBJ1_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM9430885.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6532..6852
                     /locus_tag="ACHBJ1_001958"
     CDS             6532..6852
                     /locus_tag="ACHBJ1_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM9430886.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6881..7345)
                     /locus_tag="ACHBJ1_001959"
     CDS             complement(6881..7345)
                     /locus_tag="ACHBJ1_001959"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430887.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7477..8730)
                     /locus_tag="ACHBJ1_001960"
     CDS             complement(7477..8730)
                     /locus_tag="ACHBJ1_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM9430888.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8759..9076)
                     /locus_tag="ACHBJ1_001961"
     CDS             complement(8759..9076)
                     /locus_tag="ACHBJ1_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM9430889.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9102..9422)
                     /locus_tag="ACHBJ1_001962"
     CDS             complement(9102..9422)
                     /locus_tag="ACHBJ1_001962"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9430890.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9886..11301)
                     /locus_tag="ACHBJ1_001963"
     CDS             complement(9886..11301)
                     /locus_tag="ACHBJ1_001963"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9430891.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11318..12634)
                     /locus_tag="ACHBJ1_001964"
     CDS             complement(11318..12634)
                     /locus_tag="ACHBJ1_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9430892.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12784..13482)
                     /locus_tag="ACHBJ1_001965"
     CDS             complement(12784..13482)
                     /locus_tag="ACHBJ1_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM9430893.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13771..14760)
                     /locus_tag="ACHBJ1_001966"
     CDS             complement(13771..14760)
                     /locus_tag="ACHBJ1_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9430894.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14840..15673)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_001967"
     CDS             complement(14840..15673)
                     /gene="msrA"
                     /locus_tag="ACHBJ1_001967"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM9430895.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15848..>16303)
                     /locus_tag="ACHBJ1_001968"
     CDS             complement(15848..>16303)
                     /locus_tag="ACHBJ1_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430896.1"
                     /translation="KIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHII
                     KHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVN
                     ANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         5077 a         3285 c         2765 g         5176 t
ORIGIN      
        1 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
       61 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
      121 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
      181 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
      241 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
      301 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
      361 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
      421 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
      481 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
      541 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
      601 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
      661 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
      721 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
      781 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
      841 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
      901 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
      961 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
     1021 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
     1081 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
     1141 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
     1201 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
     1261 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
     1321 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
     1381 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
     1441 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
     1501 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
     1561 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
     1621 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
     1681 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
     1741 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
     1801 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
     1861 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
     1921 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
     1981 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
     2041 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
     2101 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
     2161 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
     2221 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
     2281 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
     2341 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
     2401 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
     2461 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
     2521 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
     2581 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
     2641 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
     2701 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
     2761 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
     2821 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
     2881 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
     2941 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
     3001 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
     3061 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
     3121 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
     3181 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
     3241 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
     3301 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
     3361 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
     3421 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
     3481 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
     3541 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
     3601 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
     3661 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
     3721 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
     3781 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
     3841 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
     3901 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
     3961 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
     4021 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
     4081 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
     4141 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
     4201 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
     4261 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
     4321 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
     4381 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
     4441 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
     4501 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
     4561 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
     4621 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
     4681 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
     4741 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
     4801 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
     4861 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
     4921 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
     4981 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
     5041 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
     5101 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
     5161 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
     5221 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
     5281 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
     5341 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
     5401 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
     5461 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
     5521 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
     5581 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
     5641 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
     5701 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
     5761 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
     5821 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
     5881 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
     5941 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
     6001 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
     6061 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
     6121 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
     6181 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
     6241 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
     6301 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
     6361 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
     6421 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
     6481 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
     6541 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
     6601 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
     6661 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
     6721 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
     6781 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
     6841 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
     6901 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
     6961 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
     7021 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
     7081 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
     7141 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
     7201 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
     7261 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
     7321 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
     7381 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
     7441 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
     7501 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
     7561 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
     7621 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
     7681 aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg
     7741 gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc
     7801 aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc
     7861 taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg
     7921 tttgttcacg ataaacggtg cgctatgtcc agctgcgatt gcattaacgt ttgcttcaat
     7981 ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa
     8041 gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag
     8101 tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa
     8161 tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa
     8221 tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt
     8281 ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat
     8341 aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga
     8401 aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa
     8461 gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc
     8521 tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa
     8581 gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga
     8641 gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg
     8701 tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt
     8761 acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag
     8821 atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt
     8881 gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg
     8941 catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt
     9001 ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat
     9061 ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt
     9121 tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca
     9181 tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact
     9241 tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa
     9301 gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt
     9361 gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc
     9421 attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt
     9481 taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac
     9541 actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta
     9601 acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat
     9661 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt
     9721 tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg
     9781 tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt
     9841 tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc
     9901 gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt
     9961 caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa
    10021 tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc
    10081 gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta
    10141 atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc
    10201 gttttctgtt atcagccaag ggatattatg ataattttct tttagataca tggctatatc
    10261 atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa
    10321 atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg
    10381 aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg
    10441 ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa
    10501 ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata
    10561 aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac
    10621 catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc
    10681 tactgcttta gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt
    10741 ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc
    10801 gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata
    10861 cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa
    10921 agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga
    10981 gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc
    11041 agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat
    11101 cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc
    11161 attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc
    11221 tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt
    11281 ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac
    11341 gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt
    11401 gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca
    11461 ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac
    11521 caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca
    11581 tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata
    11641 acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac
    11701 cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa
    11761 tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa
    11821 gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga
    11881 cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg
    11941 ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga
    12001 taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac
    12061 tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc
    12121 tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt
    12181 tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga ttttcattca
    12241 ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga
    12301 atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga
    12361 tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta
    12421 aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa
    12481 acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta
    12541 tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta
    12601 atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc
    12661 tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt
    12721 tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa
    12781 ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat
    12841 atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat
    12901 ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa
    12961 caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc
    13021 ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg
    13081 aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc
    13141 atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc
    13201 tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc
    13261 tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc
    13321 tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc
    13381 atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat
    13441 cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc
    13501 atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta
    13561 tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa
    13621 gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat
    13681 gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat
    13741 atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc
    13801 ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa
    13861 ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc
    13921 catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc
    13981 cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga
    14041 atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa
    14101 cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc
    14161 acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa
    14221 aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa tatataatgc
    14281 ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg
    14341 gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg
    14401 gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat
    14461 atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct
    14521 aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag
    14581 gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat
    14641 aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc
    14701 tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa
    14761 tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact
    14821 acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt
    14881 tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt
    14941 tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg
    15001 gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg
    15061 attgttccgc taattctttt tgtcgttcga cttgataaaa aatcactgga cgatattgct
    15121 tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg
    15181 tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat
    15241 gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt
    15301 atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga
    15361 aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg
    15421 aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta
    15481 catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata
    15541 actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc
    15601 gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt
    15661 gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta
    15721 atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa
    15781 aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa
    15841 agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg
    15901 taacaagata agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa
    15961 ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt
    16021 gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc
    16081 tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta
    16141 cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag
    16201 cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg
    16261 tttctttgtt tacaacaata tgtcccggct gagaaattat ttt
//
LOCUS       DAYYNQ010000065        16298 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000065 DAYYNQ010000000
VERSION     DAYYNQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.065"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(199..1035)
                     /locus_tag="ACHBJ1_001969"
     CDS             complement(199..1035)
                     /locus_tag="ACHBJ1_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM9430897.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1046..1975)
                     /locus_tag="ACHBJ1_001970"
     CDS             complement(1046..1975)
                     /locus_tag="ACHBJ1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM9430898.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2167..2520
                     /locus_tag="ACHBJ1_001971"
     CDS             2167..2520
                     /locus_tag="ACHBJ1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM9430899.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2594..3250)
                     /locus_tag="ACHBJ1_001972"
     CDS             complement(2594..3250)
                     /locus_tag="ACHBJ1_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9430900.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3476..5410
                     /locus_tag="ACHBJ1_001973"
     CDS             3476..5410
                     /locus_tag="ACHBJ1_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9430901.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5496..6719)
                     /locus_tag="ACHBJ1_001974"
     CDS             complement(5496..6719)
                     /locus_tag="ACHBJ1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9430902.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(6713..8584)
                     /locus_tag="ACHBJ1_001975"
     CDS             complement(6713..8584)
                     /locus_tag="ACHBJ1_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM9430903.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(8605..9723)
                     /locus_tag="ACHBJ1_001976"
     CDS             complement(8605..9723)
                     /locus_tag="ACHBJ1_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM9430904.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(9806..10639)
                     /locus_tag="ACHBJ1_001977"
     CDS             complement(9806..10639)
                     /locus_tag="ACHBJ1_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430905.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(10632..11549)
                     /locus_tag="ACHBJ1_001978"
     CDS             complement(10632..11549)
                     /locus_tag="ACHBJ1_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9430906.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(11572..11751)
                     /locus_tag="ACHBJ1_001979"
     CDS             complement(11572..11751)
                     /locus_tag="ACHBJ1_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430907.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(11776..12900)
                     /locus_tag="ACHBJ1_001980"
     CDS             complement(11776..12900)
                     /locus_tag="ACHBJ1_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM9430908.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13083..13991
                     /locus_tag="ACHBJ1_001981"
     CDS             13083..13991
                     /locus_tag="ACHBJ1_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430909.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14132..15745
                     /locus_tag="ACHBJ1_001982"
     CDS             14132..15745
                     /locus_tag="ACHBJ1_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9430910.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5145 a         3185 c         2721 g         5247 t
ORIGIN      
        1 ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt
       61 tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa
      121 tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg
      181 tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac
      241 tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc
      301 tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg
      361 catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg
      421 gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct
      481 gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata
      541 gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat
      601 ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt
      661 ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc
      721 tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc
      781 tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg
      841 ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc
      901 caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata
      961 ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat
     1021 caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg
     1081 caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta
     1141 gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga
     1201 acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa
     1261 aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa
     1321 ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac
     1381 gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca
     1441 gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat
     1501 tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa
     1561 gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct
     1621 gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag
     1681 cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa
     1741 cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa
     1801 aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata
     1861 cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga
     1921 tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt
     1981 tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac
     2041 aagttgctct gtaaattttt acacaactct tattttttac caaagcactt caattgactt
     2101 tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga
     2161 ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc
     2221 gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga
     2281 ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca
     2341 ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa
     2401 ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta
     2461 gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa
     2521 tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa
     2581 tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga
     2641 aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg
     2701 gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact
     2761 gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt
     2821 ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc
     2881 gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat
     2941 ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg
     3001 tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac
     3061 catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat
     3121 ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta
     3181 aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat
     3241 gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata
     3301 gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta
     3361 ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta
     3421 attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat
     3481 attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat
     3541 acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa
     3601 atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa
     3661 aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac
     3721 agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg
     3781 cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt
     3841 aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa
     3901 tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat
     3961 cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca
     4021 ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg
     4081 gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta
     4141 ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta
     4201 caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa
     4261 ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct
     4321 tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga
     4381 ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa
     4441 gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac
     4501 cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc
     4561 gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg
     4621 tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga
     4681 tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga
     4741 aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc
     4801 aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa
     4861 caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga
     4921 agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg
     4981 atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt
     5041 ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga
     5101 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
     5161 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
     5221 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
     5281 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
     5341 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
     5401 tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt
     5461 tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta
     5521 gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc
     5581 ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt
     5641 gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc
     5701 ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc
     5761 accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa
     5821 caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca
     5881 aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct
     5941 tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt
     6001 tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa
     6061 taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt
     6121 tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct
     6181 tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa
     6241 cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt
     6301 attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg
     6361 ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga
     6421 aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct
     6481 gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat
     6541 tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg
     6601 atctcattat tccattcttt ttccattaat tctttataac attcatttat ccatttttct
     6661 ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata
     6721 attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat
     6781 caattttggg acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca
     6841 ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag
     6901 tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt
     6961 taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt
     7021 tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc
     7081 ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc
     7141 cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg
     7201 caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat
     7261 aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc
     7321 ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac
     7381 caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg
     7441 gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat
     7501 tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc
     7561 aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag
     7621 ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca
     7681 ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta
     7741 accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa
     7801 agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt
     7861 ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc
     7921 taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag
     7981 gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac
     8041 aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt
     8101 tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat
     8161 attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt
     8221 tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac
     8281 gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt
     8341 ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg
     8401 ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc
     8461 cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat
     8521 ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt
     8581 tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag
     8641 taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct
     8701 atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata
     8761 gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac
     8821 agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt
     8881 atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat
     8941 tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt
     9001 gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga
     9061 attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa
     9121 ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc
     9181 atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag
     9241 taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc
     9301 tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc
     9361 tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa
     9421 taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg
     9481 ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca
     9541 taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc
     9601 tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc
     9661 atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt
     9721 catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact
     9781 agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa
     9841 atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta
     9901 gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa
     9961 ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat
    10021 tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta
    10081 cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca
    10141 gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa
    10201 tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa
    10261 ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca
    10321 acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg
    10381 ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata atcaagggat
    10441 aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat
    10501 tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat
    10561 atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt
    10621 gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata
    10681 acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca
    10741 aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa
    10801 gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact
    10861 gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat
    10921 ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca
    10981 gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat
    11041 attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa
    11101 aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc
    11161 gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt
    11221 gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct
    11281 gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct
    11341 agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca
    11401 aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc
    11461 aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt
    11521 gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc
    11581 agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc
    11641 aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga
    11701 ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca atagttcata
    11761 gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa
    11821 tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag
    11881 cataaatgta agaaattgtt gtgccacttg tggatttttt gcatctttag ggacataaaa
    11941 acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg
    12001 agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca
    12061 gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta
    12121 gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc
    12181 tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag
    12241 catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt
    12301 tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa
    12361 atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc
    12421 actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg
    12481 atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc
    12541 atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg
    12601 catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc
    12661 tacattcaca atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc
    12721 attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt
    12781 ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact
    12841 taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat
    12901 gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac
    12961 ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca
    13021 tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga
    13081 aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct
    13141 attccataga tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg
    13201 aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc
    13261 ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa
    13321 cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat
    13381 ctttttctat agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca
    13441 aaattgatta tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt
    13501 ttttcatact atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc
    13561 tgcaaaaaat tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga
    13621 caataccttc aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat
    13681 tagttgatcc aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac
    13741 aattagccca aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga
    13801 atggaaaaag tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg
    13861 tgctagaaaa tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc
    13921 atcatttttc ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat
    13981 ccttaaaata gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt
    14041 ctattcaaaa taaacaataa ttttccggca caattatttg ctgtttttta ccataacata
    14101 gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc
    14161 tggcggtcct tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat
    14221 ctatgaacgc ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg
    14281 tgtgatcact gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa
    14341 caacatcgat gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt
    14401 aacagttaaa gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat
    14461 ctcttctggt gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg
    14521 ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa
    14581 tccacaacat gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt
    14641 caagcatcgc ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta
    14701 tgatccagaa attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca
    14761 tttagaaaca agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg
    14821 ctccgtcctt tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt
    14881 ggtgaaagca accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt
    14941 acaaaccaat ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt
    15001 gactggtcag ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct
    15061 ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc
    15121 aaaaatcttt gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc
    15181 aggcataaca aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc
    15241 agaagctaca agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg
    15301 tggacaagca tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat
    15361 caccggaaac ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta
    15421 ttctactacc cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa
    15481 tatcgtagaa acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt
    15541 tttagatata cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat
    15601 tccaatgaat gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat
    15661 tactttcaga agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat
    15721 caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag
    15781 ttatttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata
    15841 tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata
    15901 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
    15961 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
    16021 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
    16081 cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac
    16141 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
    16201 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
    16261 ttaaataatt taattttttt gttcctattt atttaaca
//
LOCUS       DAYYNQ010000066        16166 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000066 DAYYNQ010000000
VERSION     DAYYNQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.066"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACHBJ1_001983"
     CDS             <1..435
                     /locus_tag="ACHBJ1_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9430911.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACHBJ1_001984"
     tRNA            complement(759..842)
                     /locus_tag="ACHBJ1_001984"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACHBJ1_001985"
     CDS             1370..1870
                     /locus_tag="ACHBJ1_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9430912.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACHBJ1_001986"
     CDS             complement(2060..2404)
                     /locus_tag="ACHBJ1_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM9430913.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACHBJ1_001987"
     CDS             complement(2558..3478)
                     /locus_tag="ACHBJ1_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9430914.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACHBJ1_001988"
     CDS             3665..4156
                     /locus_tag="ACHBJ1_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM9430915.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACHBJ1_001989"
     CDS             complement(4180..6555)
                     /locus_tag="ACHBJ1_001989"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM9430916.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACHBJ1_001990"
     CDS             6881..7507
                     /locus_tag="ACHBJ1_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9430917.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACHBJ1_001991"
     CDS             7530..8234
                     /locus_tag="ACHBJ1_001991"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9430918.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACHBJ1_001992"
     CDS             8426..9259
                     /locus_tag="ACHBJ1_001992"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM9430919.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="ACHBJ1_001993"
     CDS             9456..11384
                     /locus_tag="ACHBJ1_001993"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9430920.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="ACHBJ1_001994"
     CDS             11522..11977
                     /locus_tag="ACHBJ1_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM9430921.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12057..12185
                     /locus_tag="ACHBJ1_001995"
     CDS             12057..12185
                     /locus_tag="ACHBJ1_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430922.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12447..12716
                     /locus_tag="ACHBJ1_001996"
     CDS             12447..12716
                     /locus_tag="ACHBJ1_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430923.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /gene="rpsT"
                     /locus_tag="ACHBJ1_001997"
     CDS             13116..13370
                     /gene="rpsT"
                     /locus_tag="ACHBJ1_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM9430924.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACHBJ1_001998"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="ACHBJ1_001998"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM9430925.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..>16166)
                     /locus_tag="ACHBJ1_001999"
     CDS             complement(14543..>16166)
                     /locus_tag="ACHBJ1_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM9430926.1"
                     /translation="YKDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIG
                     WPLHVSIIPLSLLTICYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFW
                     LLLLFDPRFLFHAGGQLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYE
                     WPILGSLFTLLIAPVFKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSG
                     SSLIIGRLPLVLSGMYIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIA
                     FIDVGQGDSIFLQSPFRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGI
                     SVIDKLILTHDDTDHVGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEII
                     EIKHGDRISGYYDLYVLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLL
                     IYPNLKADVVKLGHHGSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEEN
                     QIKTWRTDQQGMITYRWHPVFHPHGLVETMID"
BASE COUNT         5033 a         3091 c         2860 g         5182 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaaca aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatag
//
LOCUS       DAYYNQ010000067        15199 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000067 DAYYNQ010000000
VERSION     DAYYNQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.067"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..716)
                     /locus_tag="ACHBJ1_002000"
                     /pseudo
     CDS             complement(18..716)
                     /locus_tag="ACHBJ1_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            795..971
                     /locus_tag="ACHBJ1_002001"
                     /pseudo
     CDS             795..971
                     /locus_tag="ACHBJ1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1147..1716)
                     /locus_tag="ACHBJ1_002002"
     CDS             complement(1147..1716)
                     /locus_tag="ACHBJ1_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9430927.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1993..3078)
                     /locus_tag="ACHBJ1_002003"
     CDS             complement(1993..3078)
                     /locus_tag="ACHBJ1_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430928.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFAMNTKKQEDLLGY"
     gene            3405..4280
                     /locus_tag="ACHBJ1_002004"
     CDS             3405..4280
                     /locus_tag="ACHBJ1_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9430929.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4294..4617
                     /locus_tag="ACHBJ1_002005"
     CDS             4294..4617
                     /locus_tag="ACHBJ1_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430930.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4614..5366
                     /locus_tag="ACHBJ1_002006"
     CDS             4614..5366
                     /locus_tag="ACHBJ1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM9430931.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5356..7527
                     /locus_tag="ACHBJ1_002007"
                     /pseudo
     CDS             5356..7527
                     /locus_tag="ACHBJ1_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACHBJ1_002008"
     CDS             complement(7639..9810)
                     /gene="copB"
                     /locus_tag="ACHBJ1_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM9430932.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9830..12016)
                     /locus_tag="ACHBJ1_002009"
     CDS             complement(9830..12016)
                     /locus_tag="ACHBJ1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9430933.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACHBJ1_002010"
     CDS             complement(12016..12225)
                     /gene="copZ"
                     /locus_tag="ACHBJ1_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM9430934.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12238..12678)
                     /locus_tag="ACHBJ1_002011"
     CDS             complement(12238..12678)
                     /locus_tag="ACHBJ1_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM9430935.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12752..13250)
                     /locus_tag="ACHBJ1_002012"
                     /pseudo
     CDS             complement(12752..13250)
                     /locus_tag="ACHBJ1_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
     gene            complement(13535..15001)
                     /locus_tag="ACHBJ1_002013"
     CDS             complement(13535..15001)
                     /locus_tag="ACHBJ1_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM9430936.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4322 a         3166 c         2687 g         5024 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaagacgatt ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt
     2101 cagtaaaagc atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg
     2161 aaattgatat agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg
     2221 aacttcttct atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag
     2281 tacttttatc agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga
     2341 atcaagcttt tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt
     2401 ccaccgcttt tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc
     2461 ttttattggc aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag
     2521 tgttcgactg tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg
     2581 gatgaacatc tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt
     2641 caaaaagtaa aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt
     2701 ataagaaaaa gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg
     2761 atcgtacttc tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg
     2821 tgattggatc agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa
     2881 ctgaacggtt ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt
     2941 cagatgccga tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg
     3001 ccagatcaaa tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa
     3061 tagtttttgc catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa
     3121 aatgagttca ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc
     3181 ttcctcatta ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac
     3241 tttaaaaaaa cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga
     3301 aataaatagc gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca
     3361 aacaaaaaat cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa
     3421 ctatccaatt gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc
     3481 ccggtgtagg taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca
     3541 gcagagtcca atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca
     3601 attcacaatc aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg
     3661 cagatgagat caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg
     3721 agaatcatgc aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag
     3781 ctacgcaaaa tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc
     3841 gttttttatt ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt
     3901 tggataaatt cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag
     3961 aagaaataaa aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct
     4021 tgcagttagt gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag
     4081 gaagcctttc ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg
     4141 tcacgcccaa agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt
     4201 acgatcgttc attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa
     4261 ttcctattcc tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt
     4321 aattttgtgt tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat
     4381 tctttaggct ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca
     4441 ttaagcagct ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc
     4501 aaaaaaagta tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc
     4561 cctcgtttac aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc
     4621 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     4681 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4741 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4801 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4861 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4921 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4981 ctcttttttg cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt
     5041 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     5101 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     5161 aagctttttt cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     5221 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggcttatc
     5281 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     5341 caggaggtga atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt
     5401 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     5461 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     5521 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg
     5581 aagacatttt ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc
     5641 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     5701 ttgctgatct atttatttat tacattgacc gtggattacg actattggta cgcacccttt
     5761 ctttctttga tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac
     5821 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5881 atccggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5941 ttcttaactt ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     6001 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat
     6061 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     6121 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     6181 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     6241 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa
     6301 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     6361 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     6421 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     6481 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     6541 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     6601 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat
     6661 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccgggcatac tccgaaagaa
     6721 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6781 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6841 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6901 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6961 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     7021 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     7081 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     7141 ttattcagcc ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa
     7201 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     7261 gcatatcaaa aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct
     7321 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     7381 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat
     7441 aaaaaaacct atttagaaat aacgcgtata ataagaaaaa cggtatacaa aaaaattctg
     7501 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     7561 ccgagaacca gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt
     7621 cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac
     7681 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7741 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7801 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7861 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7921 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7981 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     8041 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     8101 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     8161 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     8221 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     8281 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac
     8341 tgttgcctct agcccgactc ctgaaagatt ttttagattt tcagcttgat aaggcttgat
     8401 ttcatgttct ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc
     8461 caaggctcct agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     8521 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     8581 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     8641 tattgatgtg gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     8701 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8761 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8821 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8881 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8941 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     9001 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     9061 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     9121 ctcttctttt ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt
     9181 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     9241 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     9301 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     9361 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     9421 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     9481 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     9541 agctaggata agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc
     9601 catgccgccg tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     9661 atctccatgg ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat
     9721 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc
     9781 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9841 tgtgtcgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9901 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9961 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga
    10021 ttgtttgtgc aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg
    10081 caatgtccgt tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat
    10141 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
    10201 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
    10261 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
    10321 ctttattctg gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg
    10381 caatccaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
    10441 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
    10501 tacgtgtacc cgcaaaatag cgtactccat taatcgtacc gctgatccca gcacctgggt
    10561 gggctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
    10621 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
    10681 cttttgaacc gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat
    10741 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10801 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggggtc gctaaaccta
    10861 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10921 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10981 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    11041 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca
    11101 tgtgtagcag accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt
    11161 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    11221 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    11281 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    11341 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    11401 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    11461 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    11521 ctaccaatac atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa
    11581 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    11641 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    11701 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11761 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccataaccga
    11821 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11881 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11941 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    12001 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    12061 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    12121 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    12181 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    12241 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    12301 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtagcttc
    12361 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    12421 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    12481 tcctttttgt tctgtccaga tcacttcttt ttttaccaaa cgtcccagca aagttttgat
    12541 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    12601 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg
    12661 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attagcttat
    12721 gaatacagat tacgcttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12781 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12841 tagctacttg gatcagctgc tgtatttttg gttcattgtt tccttcaaaa tgccagataa
    12901 aagataaaaa ttctaagtat ttcctgttta gtttctccga aaatgattca ggcatatctg
    12961 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    13021 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaacg
    13081 tagaagtata atttccttca acgatccatg acggattaga tactataaac gattgctgtt
    13141 cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt
    13201 gcgagggacg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    13261 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    13321 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa atggtgttca atcagctgac
    13381 cttcagcatt agctcaaaaa acggcaaaat atgaggagct atttttgttt ggctgttctt
    13441 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc agaagtagct
    13501 tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact gtttctggtt
    13561 cagtaatttc tttgtttttg tctcgacgag caattgctgg caagatcata cctaataaga
    13621 tcaagacaat tggtgtgatg atattcgtga ataattggaa ccaccatgct ttaggatcag
    13681 cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg aaggcaaagc
    13741 accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat tcagaattga
    13801 atttcttata agctttattc aacatcatat atgccaagaa cacccatagg taacgcattg
    13861 gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata tctttgatac
    13921 caaagatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga taaccattca
    13981 ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct ggatccgcat
    14041 ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag atttgtccaa
    14101 tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag taattaccta
    14161 aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttagggata ttatcgctag
    14221 caaacagcat acccattgcg actgagccta atacagcaca aatccctacc attgctgcta
    14281 agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg acataagggg
    14341 atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg aaataactga
    14401 aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca aattccatcg
    14461 ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac atgacgaaca
    14521 tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt gttgataaat
    14581 ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact gacatcgtat
    14641 ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt gcttgaggct
    14701 tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag tatgccagtc
    14761 gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta tccttgaagg
    14821 ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc aaaatcaata
    14881 gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca ttccccatgc
    14941 cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt ttgttgttca
    15001 ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt caatttttaa
    15061 caaacttaca agacagtgtt attttatgtg tgtattattt tatttaatac acatcgtttt
    15121 ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga ccttattgaa
    15181 tttcaagcta ttttgaatt
//
LOCUS       DAYYNQ010000068        14818 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000068 DAYYNQ010000000
VERSION     DAYYNQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.068"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..1770
                     /gene="yfcC"
                     /locus_tag="ACHBJ1_002014"
     CDS             31..1770
                     /gene="yfcC"
                     /locus_tag="ACHBJ1_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM9430937.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1927..3111
                     /gene="iadA"
                     /locus_tag="ACHBJ1_002015"
     CDS             1927..3111
                     /gene="iadA"
                     /locus_tag="ACHBJ1_002015"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM9430938.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3222..3425
                     /locus_tag="ACHBJ1_002016"
     CDS             3222..3425
                     /locus_tag="ACHBJ1_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM9430939.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3526..4188)
                     /locus_tag="ACHBJ1_002017"
     CDS             complement(3526..4188)
                     /locus_tag="ACHBJ1_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430940.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4201..5703)
                     /locus_tag="ACHBJ1_002018"
     CDS             complement(4201..5703)
                     /locus_tag="ACHBJ1_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430941.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6251..7930
                     /locus_tag="ACHBJ1_002019"
     CDS             6251..7930
                     /locus_tag="ACHBJ1_002019"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9430942.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8057..8536
                     /locus_tag="ACHBJ1_002020"
     CDS             8057..8536
                     /locus_tag="ACHBJ1_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM9430943.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8755..9435
                     /locus_tag="ACHBJ1_002021"
     CDS             8755..9435
                     /locus_tag="ACHBJ1_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM9430944.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9443..10774
                     /locus_tag="ACHBJ1_002022"
     CDS             9443..10774
                     /locus_tag="ACHBJ1_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM9430945.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10789..11751
                     /locus_tag="ACHBJ1_002023"
     CDS             10789..11751
                     /locus_tag="ACHBJ1_002023"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM9430946.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11862..12200
                     /locus_tag="ACHBJ1_002024"
     CDS             11862..12200
                     /locus_tag="ACHBJ1_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM9430947.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12279..12986
                     /locus_tag="ACHBJ1_002025"
     CDS             12279..12986
                     /locus_tag="ACHBJ1_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM9430948.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13089..14570)
                     /locus_tag="ACHBJ1_002026"
     CDS             complement(13089..14570)
                     /locus_tag="ACHBJ1_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430949.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4696 a         2546 c         3178 g         4398 t
ORIGIN      
        1 attactgaaa aaagaaagga agaaataaat ttgaagaaaa caaattcttc aggaaaaccg
       61 aaacgcgact tcactaaatt atcgacaccc catacgtatg tcattatttt tggtgtagtc
      121 atctttgcat ggatactgac ttttgtcgtt ccagcaggaa agttcagcac gcaagatatt
      181 gaatacaaag atgcaaatgg cgaaacaagt actcgtactg ttttacgtca agattccttc
      241 cgttatgctt acgagctgga taagtcgtat gtgtttgatc aattagaaga actgcaagat
      301 catccagcag aacgtgaaaa actggatgtt ccggaaaaag gattggaaaa agtcattgct
      361 gacggagaaa aaaatcttac gcaggaaaaa ctagatgaaa tctccttgac agatgatgtg
      421 ctttacgatc agtacggcga aaatatctat gatacttcta aaaaattgca taaaacagcc
      481 aaaatatggg gaacagatga ttttggcggc tttggtttct taaattttgt ttttgaagga
      541 ctagtatctg gagataagta tggatctgct gtagggatcg ctgcattgat ccttgttgtt
      601 ggaggtgctt ttggtattat tatgcgtacc ggagcgatcg acgcaggtat ctatgcgttt
      661 atcagtaaaa ctaagggact ggagcgttta gctttaccgc tattattctt tgctttttca
      721 ttcggtggcg ctacattcgg gatggcagaa gaggtaattc cattctcaat ggtgatggtt
      781 ccatttgtca ttgcgttagg atatgactcg atcgtagcag taaccgtgac ttatgtcgca
      841 tctcaagtcg gaaatgcgac ctcttggatg agcccgttta gcgtagcggt ggctcaaggg
      901 attgctggaa tccccgtgtt gtctggtgcg acattccgat tgattatgtg ggtagttgtt
      961 actgcattag ccgctggtta tatgatgata tatgcagaaa aaatccgtaa aaaaccagaa
     1021 agttcgttga cttacaaatc agatgattat ttccgttctc atatcaaaaa gacatctgat
     1081 gaaaataaaa aattcatgct tgggcataaa ttgatcttat tagagatgtt agttgtcctt
     1141 gtctggatcg tttggggcgt gactcaaaaa ggctattaca ttccagaaat cgcttctcaa
     1201 ttctttgtga tgggattagt tgctggaatc attgctgttc ttttcaaatt agatggaatg
     1261 aaagtcaatg acatcgcttc ttctttccaa tctggtgcag ctgatttagc tggtacagca
     1321 atcgtagtag gtatggccaa agggattctt cttgtcttgg gaggatctga tgcatctgtt
     1381 ccttcagctt tgaatacgat tttgcatggg atcgggacgg cgttgaccgg tgttccagct
     1441 gtcattggtg cttgggcgat gtacatcttc caaagtttgt tcaacttggt tgttacatca
     1501 aattcaggac aagcagcttt gactatgcca atcatggctc cactggcaga tttagtaggt
     1561 gtatccagac aagtagcagt tcttgcttat cagcttggtt caggttttat ggacgctttt
     1621 acacccgtat cagccagctt gattggtgtg ctaggtgttg cacggatcga atgggcaaaa
     1681 tgggcaagat tccaaatcaa gatgcaagga ttcctgtttg ttttaggaac tattttcatc
     1741 atgattgcca ttgcaatagg tctgcagtag tactggagac ctgtaaataa aaaagtaatc
     1801 caaacagtag ataaggactc caagaaccgt cattttacgg taaaatcaaa ggagtaaaga
     1861 tcctatcgtt tggattattt tttaagatcc tatcatcagt tatacttaaa gaaagaggaa
     1921 aaatgtatga aaatcattcg acaaattgaa gtgtttgcac cagattactt aggaaaaatg
     1981 gatgtactga ttgccggtgg gaagattttg gctgtagaag aacagctaga aggcggctac
     2041 gaaggagtgg aagtagaaga actttcaggt gagggaaaag tactaacccc gggtttcatt
     2101 gactgtcatt tccatatttt aggtggagga ggagaaggcg gttatcaaaa tcgaacgcca
     2161 gaagtcacat tgagccaatt gactacagct ggtgtaacga cagttgtcgg ttgtttggga
     2221 acagacggag aaggtagaga tatgactgcg ttgatcagca aagcgaaagg tctggaagca
     2281 gaaggaatct ccacttatgt atacgaaggt tcgtaccgat tgccggtaaa aacagtaaca
     2341 gattctatca tcaaagactt tttgacaatt gataaaatta tcggaatcgg agaaatcgca
     2401 gtatccgatc accgttcttc tcagccaagt tttgaagagt tcgctcgtgc agtagcagat
     2461 gctcgtgtag gaggcatgtt atccggaaaa gctgggatca tcaatgtcca cttaggcgga
     2521 ggaaaaagaa agctagagct attgacacga atcgtagaag aaacagaaat tccgatcaca
     2581 caatttttgc ctactcatgc aaatcgcaca ccagaattat ttgaagcttg cgtggcattt
     2641 gccaaacgtg gtggcacgat tgacttcact gctagcgaag atcctgattt ttgggagaag
     2701 acagatggtg aagttcgctt cagcaaagca ctgaaacgat tgatcgagga agatgtttct
     2761 ttagataatt tcacgatgac ctcagatgga caaggaagct tgccttattt tgatgaaaac
     2821 aatcatttcc tagggttagg agtaggaagt gccaaggcat tgctagtagg gatcaaagaa
     2881 gctgtgcaaa aagaaagtat ccctttggaa atcgccttac gagctataac cagtaatcca
     2941 gcacgtatat tgaaattgga taaaaaagga aaaatcgaaa ttggtgctga tgcagatctg
     3001 tgtatcttgg ataaagaaac gcttgatatt gatacagtta tcgctaaagg agaaataatg
     3061 gtccaagaaa aagaagtcaa agtatggggg actttcgaaa aaagttttta atcaacagct
     3121 ctaactatct tttttagaaa acgagcgttc tgtttgtctg tatctgttgt tgatgttacc
     3181 ctgaaggtag atcaataaac aaactaagag gaggaagcac aatggcagat ttaaaaggac
     3241 gttctaaaga tatgaaagat aaagtagtcg gtgaaggaaa agaagcatac ggcaaagcaa
     3301 cagacgacaa gagcaaagaa acagaaggga aagcacaatc actaggtgct gacatcaaag
     3361 aaaaagctcg aaatttaggc gacgaagtca aagacaagtt ttctcgaaaa gacgaaaaaa
     3421 aataaaaacc acttattact aattgaatca agcaaataaa aaatccgaac catatctgcg
     3481 agacagatat ggttcggatt ttttatttga tcattatcct gttttttaaa cttcgatttt
     3541 ttgaaatttt ttatttttta gttcaaagac ttcatcacag ctttttgcta cttcaatctc
     3601 atgagtcacg atgataatac agcgattttg ttcatgggcg atttcttgga aaagtttgac
     3661 gatatcttgt gaagttgttt catctagatt tccagtaggc tcatccgcta cgattagttg
     3721 atgttcacag cagatagcac gaacgatagc gactcgctgc tgctgacctc ccgaaagttt
     3781 ggtcacctgt ttttttgcta atatttcatc aattcctaca cgagccagtc gatctaaagc
     3841 aacttgtcga taattctctt gcttggtctt agtgatttcc attgctgcta tgacgttgtc
     3901 tagagccgat aaataaggaa gcaagttgta agcttggaaa ataatcgaga catcattttt
     3961 acgataactt cttaacccga ttttgttcag tgaacgatct tgatagtata cctttccttc
     4021 ttttggtgca tctagtccag aaataagcga aagaaaagtc gtttttccag aaccacttgc
     4081 tcctaaaata gcgtacattt ttccagcttc gaaattcagt tctacatctt tataaagcgc
     4141 atcttgcgga tcttgatacc aaaatcccaa ttgttctgtc cgtaacatca aattcctcct
     4201 ttagtttaat aggatttttc ttgggtttaa tcttaatatc cctatagaag ctaaaaagac
     4261 agaaaacaga ctgattccaa atgctactcc ggctaatagt cccaattgtg caggtttgac
     4321 attgatttcc aattcgctga cttgatcaga aacagcaaat ggattattcg tggttccttg
     4381 ttgaccatcg cgttgaccgc cttgttcttg tctgttacct ggcatttgtc caccgccagg
     4441 agcttgctgg ttttgtgtcg tggtttctgt ctgttggtca agtagctgct gtcccacggc
     4501 attcgcgacg acgtttccgc taagcgaagc aactcctaat gcaagaatca tacaaatagc
     4561 cacttcggta aacaattgga gaatgatttt acttctggat tctcctaaag atagcaagac
     4621 gcctaactca tgccgacgct cccgaatggt aatcataatg atcagagtaa gtatgataat
     4681 accggctaca gccactagta aaacaacatt tttagaaaaa cttgccacgt tgtttaaagg
     4741 ttccaacatt gactgataca tggaatcatt tgattgtaag ctaaatgtat ctgtgtcgat
     4801 cagcttctca gcttctttga gaaactcatc cattttatct ggatcagata gagtgtaaat
     4861 agcagaatct atcgtgttat cgtcagatgt cccatttagt tcatttgcaa aagtgtaaga
     4921 agtgtaaagt gtattcgacg gattcatgaa gttgaatttc atacccatac tgccgcttgt
     4981 ttcgctggat tcataaattc ctttgatcgt catttcataa gtggtatctt catcttcagt
     5041 actcgtgatt gtgaaagtat ctccgactgc caggtcattt gtttctgcta gagtagaatc
     5101 aatgactaca ttgtttgtgc ctttgtcgct gtcatctatt ccttcaccat ccgtgatttt
     5161 tgctgttcca tcagaaaaat tactattttg tgcagttgct gacacaccac tgatttgaaa
     5221 gtctgcttga gtcatttgcg gattcccgcc cattcctcca gcaggttcac cgcctgcatc
     5281 agtagtttct gaactgtctt cacttgaatc agaactagtg atggcagtta ttccatcttt
     5341 tgccatagct gaggcggaac tttcaaagga ataactcttt acattatcta attcagctat
     5401 tttttccgcg tccgttaagg aaactggtgt aagagaaaaa ctgcctggat ctggacgttc
     5461 ttcagtggta gaaccgcttg aagaagtttg ttcctgtttt ttaaatgaag cttctcgatt
     5521 ggcacttaaa gtcactgttg ctccaacttc tttttgtgct tgattcgctg cttcttctgc
     5581 agcgcttcga atcgtgaggc cagctagcac aaaaataaga atggctgtga acacggcaaa
     5641 caataacaat gaacgtcccc attttacttt tgtacttttc catgcacgtt tcataaaatt
     5701 catattttgc atccctccta gtagatgcat ttgataatag acaacctatc ttaaagctag
     5761 cttaaatctt atgctgtttg cacaccatca acatgaggtt ctgtatatat aaaaagtcac
     5821 tatagttatt gcaaaaaatg tggtaaaatg aaaaaagtat gtttcaaacc gacgaagaag
     5881 aaatgaaaga ggaatagcaa aagaaatcct agatgctaca gagggatttt tacgtgcata
     5941 gttgttctaa caattgctga tactagcgat ttagtttatt tgttgtataa taatggggaa
     6001 cagctgaata cttatataag tattctggct atagcgtttc gcaaaagatc cattttgtta
     6061 atgaagtata tccaaaataa aatgcccatc caactgtcag aacgcggatg tgctggtaat
     6121 tttcctgctg attctccata agttgagaaa tagtgatttt gcatatgctt cattggaatg
     6181 gctcatatat ttagataaag aagaaaatcg acatctttaa acttggatgt cataagaaga
     6241 ggtgaagttt gtgagtacca ttaaaattat cccattaggc ggcgttcgtg aaaatggaaa
     6301 aaacatgtat gtcgcagaag tgggcgagga tatttttatt ttagattgtg ggctaaaata
     6361 tcctgaaaat gagttgttgg ggatcgacgt cgtaatccct gattttacgt atttagaaga
     6421 aaacattgat cgtgtagctg gtgtcttttt aacacatgga catgcagatg cgatcggggc
     6481 acttccttac ttgttgtcaa aaatttctgt accagtattc gggacagagt tgacgatcga
     6541 gttagcaaaa ttgaatgtca gtcgtaatga agcagccaaa ggatttaagg atttccatgt
     6601 ggtagatgag catacagaaa tagactttgg tgaaacagta gtgagctttt tccgtaccac
     6661 tcatacgata ccagattcag tggggatcaa tctgaaaaca aaagaaggta gtatcgttta
     6721 tacgggagat tttaaatttg accaaacggc tatcccaatg tatcaaacag attatgcacg
     6781 attggcggaa atcggcaaag aaggagtctt agccttacta agtgaatcgg caaatgcaga
     6841 aaatcctcag cctgtagctt ctgaagccca aatagcagat gaagtatttg atacgatcaa
     6901 atattgggaa gggcgtatca tcgtcgcttg tgtagcaagt aatttgcaac gagtgcaaca
     6961 agtattggat gcagcagaaa aagcacaacg aaaagttgtc ttgactggtc aagatttcga
     7021 acgaattatt cgcacagcga tgcgtttaga aaaacttcaa ttaccaagtg aagatctgct
     7081 tgttacgttg aaagacatga aaaactatga tccagaagaa ctgatcattt tagaaactgg
     7141 ccgaatgggt gaacccatca agtctctgca aaaaatggca aacggtacac accgtaatgt
     7201 caagattgaa gaaggagatt tggtttatat cactactacg ccaagcatcg cgatggaaac
     7261 catcgtggcg aaaacggaag atatcattta tcgagctggt ggaacagtga agttgatctc
     7321 tgagaatatg cgcgtttctg gccatgccaa tcctaatgac ttgcaattaa tgatcaattt
     7381 gatgaaacca acatattttg tgccagttca aggagagtat cgtgaattag ctgctcatgc
     7441 agatttggct catgcagtag ggatgccata caaaaatatc tatattactg gacgcggaga
     7501 cgtgctggaa tataagaaca agcgtatgag tgttgcagga actacaagtg cagaaaatgt
     7561 aatgattgat ggattaggca taggtgatat cggaaacatt gttttacgtg atcgaaaaat
     7621 cttgtcagaa gacgggattt tcgtagcggt tgtcactatc agtcggcgag aaaagaaaat
     7681 catttcaaaa cctcaaatta cttcacgggg attcgtttac gtaaaagcta gccgtgatct
     7741 gatgaaagaa agcagcaata tgattgaaac aatcgttgag aaacatttag aaagcaatga
     7801 ttttgagtgg agcaaattaa agcaagacat ccgagatcaa ttaggccgtt acttatttga
     7861 acaaacaaaa cgccgcccag tgatcttgcc agtcattatg gaagctactc aacgtcgaaa
     7921 taaaaaataa acaaaaagag taagatgaac ctctctatag agtgctcgtc ttgctctttt
     7981 tttgcgaaaa acaaataatt tttgaataca gcatccctcg ctttataata aacgatagaa
     8041 taaagggagt gaatcaatga tgagtcaaac aaaagtcgaa cgttatttag aagaacaaaa
     8101 gatcagctat caaccacttg attttagtca tgtggtagaa gaatctttca cagaagaact
     8161 acaaaaaaga gggattgatc cagctttgat atgtaaaact ctcgtagtga aaggaaacaa
     8221 aactggggtg atcatcgcat tgattccttt gaacgatcac ttagattaca aaaaaacgag
     8281 acaagtgacg aaagatcgta aagttggttt tcctggaatg gattttgtat taatgcatac
     8341 aggatatccc cacggagcaa acacccctat tggaataaaa ctcgcacatc ctgattattg
     8401 ttttttagca gacagttctt taaaagaaaa agaagaagta attgtgtcaa gcggtgaaat
     8461 cgggagaagc cttcggataa aagtagccga tttagaaaag atcgtccatc ctatttacgc
     8521 tgatttaatt agttaaggga cgagaagaga agctaagcag agcagagacc tcaaacttga
     8581 gattatatct gcttggtact tggtgcatcc tgtattatct gttacaattg ctttgtacat
     8641 actgtactat caatccaatg aaaatatatt tagcagctat attttttcat tgatgtgcaa
     8701 gtgatgaaat cgtttttaat aaaaaagata cagagaaaac gcgaggtgta aaatatgaaa
     8761 attacgtatc atggacattc ctgtttaaca attgaaatgg aaaatggcca acgcgtgatg
     8821 attgacccgt ttattacagg aaatccattg acagacttaa ctgcagaaga agtaaagaca
     8881 gattggttgc tgattactca tggtcatagt gaccacatag gagatatgat tcctattgca
     8941 aaagaaaatc aagccacaat tatcagtatt gttgagattg ctgattatgc gaacagcaga
     9001 ggagtagatt cattcggtat gaatatcggt ggaaaacatg cgtttccatt cggcgtggta
     9061 aaattcgtac atgcacagca tagttcaagc tatgaagtag acggtatcgt tcagtatatg
     9121 ggcgagccta gcggaattat tattcaagca gaaggaaaaa cgatttatca tgcaggggat
     9181 actgcttatt tcagtgatct aggattgctt gcagaagaat tcgatattga tgtcgctttc
     9241 ttgccgattg gcgataacta tacgatgggg ccagaagatg cagcaaaggc tgcgcaaaca
     9301 attcgtgcca agaaagtagt tccaatccat tacaatactt tccctgtcat tgaacaagat
     9361 cccgaatctt ttgtagagtt actggaagat agtgaaggaa aaataatgag cgtctcggaa
     9421 acgctggaag tgtaggcaga aaatggcgac aaaacatgat ttgattttag aatatattga
     9481 aagtttacct gttggcaatc gaatttctgt aagaagcatc gcaaaagaat tgaatgtcag
     9541 tgaaggaaca gcgtaccgtg caatcaaaga tgcagaaaat gtcggactag tttcaacaat
     9601 tcaacgagta gggaccatcc gtatcgaacg caaattaaaa aaacatattg aacgtttgac
     9661 atttggcgag gtcgtcagga tcattgaagg ggacgtttta ggcggggcgg caggattaga
     9721 caaggtcttg aataaattcg tcatcggcgc gatgactaaa catgccatgg aacgctatat
     9781 tacgccagga tcactaatga tcgtcggcaa ccgggcggaa gtccaaaaat tagcattgga
     9841 agatggcgcc gcagtattga tcacaggagg attcaatacg actgctgaga ttgcaaagct
     9901 agctgatgat cttgaaatgc cgatcttacg cacctcttat gatacattta cagtcgcaac
     9961 catgatcaac cgagcgttaa gcgaccaatt gatcaaaaaa gatatcatgc ttgtcagtga
    10021 tatctatatg ccagctgaaa agactcatta tttgcagcag acagatacga tttctgatta
    10081 tcagcgtttg tcagaagaaa cgcagcattc gcgttttcca gtcgtcaacc gccatcatcg
    10141 tttgatgggg attgttacgg caaaagatgt tttagggaaa tcgccaaacc agatcatcga
    10201 tcgggtgatg acgaaagaac caatcagtgt aaagaaaaca atgagtattg cctctgttag
    10261 ccatcaaatg atctgggatg ggctagaagt catgccggtc gtttccgatg acttgacatt
    10321 ggaaggcttg attacccggc aagatgtaat gaaagcgatg cagcttgtcc agcgacaacc
    10381 gcagattgca gatacgattt cggatcagat atctggaaat atccatacga ttgatactga
    10441 ccgtgaagga aatcgtttgg aaaaccctaa gttcaaattc gtagtagcac cacaaatggt
    10501 caatggcgtt ggaaccattt cttttggcgt acttagcgaa atcatttctg atgtcgcaca
    10561 aaaaacaatg gtgatgaacc aaaaacgaaa tatcttgatt gaacaagtga acctacatta
    10621 tttgcggctg atccaattgg aaagcgaatt agacattcgt ccacgtgtat tggaaatcgg
    10681 cagacgttcc gctaaactgg atatcgaagt gtttattgaa aacaccatcg tttccaaagc
    10741 aatcgttgtt tgtcaagtaa tggaacgttc ttagaaagca ggagtgaaat gacagtacag
    10801 caagaaatag tagatacaat aaaaaaatac gataaaatca ttatccacag gcatttgcgc
    10861 cctgatcctg atgcattagg atcacaagta ggattagccg aactattacg ggaaagtttc
    10921 ccagaaaaag aaatacgtca agtaggtgaa acgattgaag ggcttcgctt tttagcagaa
    10981 atgcaagaag ttgaagatgt cttctaccaa ggagcgctag tcatcgtgac tgatacagct
    11041 aattcaccca gaatcagtga tcaaagatat cagttggcag aaaaactgat caaaatcgac
    11101 catcatccca atgatgaccc ttatggagat cttgtgtggg taaatacaaa agcaagtagc
    11161 tgtagtgaga tgattgcaga atttgctttg atgtttcctg aatctttaaa gatgaacgtt
    11221 aaggctgcac ggttacttta tgcaggaatc gtcggagata cagggcgctt tttgtatccc
    11281 tccacgacgt cccgtacatt agagattgct tcgatattga gaaattatcc atttgatgca
    11341 agtgctttga acagagagat tgaacaaatg ccgatgaaag tcgctaaact ttccggttat
    11401 ctttatcaaa atatccaggt ggatgaaaat ggtgcaggaa gaataatcct tccgcaagaa
    11461 attcttgata aatatggaat cgatgattca gagacagctt cgatcgtttc acttcctgga
    11521 gtgattgacg aagtgctggc ttgggggatt tttgttcagc agccttcagg gtattaccgc
    11581 gtacgtttac gttctaaggg gccgatcatc aatgaattgg ctaaacgcca tcatggagga
    11641 ggccatccat tagcaagtgg tgcgaatgca aaagatttag cagaagtaga ggaaatctat
    11701 aaagagatcc aagatatatg ccaatcgtat aagagcagaa aaacagaatg atacttcgtt
    11761 cgaaaacgtg ccttttgtag taaaaatcac aaatgtatct tttcaagatc ttctttttcc
    11821 gttacaatag tgctcactaa agattttaaa ggagaaagaa gatgacatta ccaaattgcc
    11881 cagaatgtgg ctcagagtac acatatgaag atcgaggctt gttgatttgc ccagaatgcg
    11941 ggaacgaatg gaacaaagaa gaacaagaaa ctgtcataga agacggatta gtggtattag
    12001 atgcaaatgg aaaccgtctg ttagagggcg atagcgtgac agtgattaaa gacttgaaag
    12061 taaaaggtgc gtcaggtgcc attaaacaag gaacaaaagt caagaacatc cgtttagtag
    12121 aaggcgatca taatatcgat tgcaaggtag aaggatttgg accgatgaaa ctgaaatcgg
    12181 aatttgtgaa aaagaattaa agaatattca aggtcttttc acattctttt cctactacta
    12241 atgataaaat aaagtaaacg aaaaggagtg aaacgaaaat gtatggttat ggaatgtatg
    12301 gttttttaga tccgacattt cttttggtca ttcttggatt agttatttcc ggaattgcgt
    12361 cagcgtacgt caatagtact ttccgaaaat acgatcaagt gagaagcaaa aacaatgtga
    12421 ctggtacaca agcagcacaa tatatattac aaagtcaaca gataaataat gtcggtgttc
    12481 aacaaattgc cggagatctc acagataact acaattcagg taataagatg ttgagtttgt
    12541 ctcaagcaac tgcacagtct acttcagttg ctgcgatagg tgtagcagct catgaatgcg
    12601 gacatgcggt acaagatgct tcgggttatg tcccattgaa gattcgtgca gcacttgttc
    12661 cagcagctaa ctttggttcg atgatttcgt tccccttgat tatggtaggt gtattgctta
    12721 gctggaactc tacgttaatc aatatcggga ttttcgcttt ttctttagca ttacttttcc
    12781 aattggtgac attaccagtc gaatttaacg cgtctcggcg agcaatccaa attttgagtg
    12841 aaggcggact actgacggaa gaagaagtac cgatggcaag acacgtatta tttgcagcag
    12901 cactgactta tgttgcagca gcgctttcga cattcctgca gctgttgcgc ttgatcttat
    12961 tgttcggcgg caaccgaaga gattaaaata aagaagttgt gaaatcagcg ctttgatctg
    13021 agctaatacc gagagttagc tgattgaata ccatttattt ggaataagag gatgtgacaa
    13081 gaattttgtc acatcctctt tgttttgttc tcataacctt ttttcatata cagttcatga
    13141 accgctttta ttaacttgcc aaataacact gtattgtgcg ttgtatccag atagaaagtc
    13201 tccgtttcat ctgaaatttt ctcatgctga tctgtaatga tcaaatcagc atgttctgaa
    13261 tatgcggtaa ttttaacagc atctttgcga aataactgct gcaaatggct ttgaagatga
    13321 taatttcctg taaactgttt ggttatctgc aaatggatga gtaactgctc tttcgtttct
    13381 gtttgtagta agtgttggat caattgacta aggtattgac tgatttggtt tctttcccca
    13441 ttttttgaaa aaattgtatc gattttttct cttaatttct tttcctgtat aaaagaacta
    13501 gaatgattgg acgtggcttc tggaaagcaa agtgtagaaa aactactgct ggattcaccc
    13561 aacagttcga aaaaaacaac tgccaacaca agaaaataaa aataaattgg tattttattt
    13621 tctaaatggt tgagaggaaa ctgttgagtt aagtaagtca tcaattgacc gacttgtcga
    13681 caaataaggt ttttgcttgt ggaaaataaa aaaccaatct cctgtttttg tcgaaaagag
    13741 atttgatttg gaaaaaaaca atagaaaaaa taattaaagt aggctctctc tgtttctgtc
    13801 tcttcacaag ggcgactcac tgaaaaagaa gtaaagtatt ctgaataatc atgtgtatgc
    13861 tgcaataagt ccatcaattc agtgatttca gaagaagttt ttggaaaata tgcctgttgt
    13921 ttgtttcgta atatgaataa agttaacagc aagtagatat ttttttcttg ttttgaaatt
    13981 ttttctattt gctcatctgt actgttcatt tttagtgcat ttaattcaag tgaggaaaat
    14041 ggccaatcgt tacctccaaa tgtatctgaa agaaaaaagt aaagatagag acggatagat
    14101 aactcatttc cttcaagagt acagcaattt tttacagata ggatccgtaa atggaatttt
    14161 attaaggaat gattgagctg ttttgtcagt ttttcgagat aggagggcga tatataaagt
    14221 tgttcaagta aatatttttt tggtacagca gtttgtggat aattgattag taacatcaaa
    14281 tagttaaaac gattggattc ttttaggtag cataatttta acttagaaaa aacagctaac
    14341 tttttagttt gatcggtttc tttggtttgg atctctcctt tatgattaga ataaataaat
    14401 tcatcaatca ataaaaattg cttgagtgat acatttattt ttcggatcag ttctttgatt
    14461 gtccgttttg gaatatgtaa gtcattggat aattcttgta aagaacaatt atttttttga
    14521 atgaccattt gtagaacaag taactctttt tttcttgctt caattagcat aaatacctcc
    14581 tttttattct gtttttgtat atagttagac aaaaactgtg agtggaaagt catttcagtc
    14641 tgtgttattc tcacagagtg tcgccttctt ttttatataa cgttatttaa tttttttgtt
    14701 accttgttta ttctattgtt tatttcctag ggtattttgt caagatattc ttgtttttaa
    14761 ggcggaaaaa ttgattttta atggatataa ctaaatatgt tttgataatt atcaataa
//
LOCUS       DAYYNQ010000069        14695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000069 DAYYNQ010000000
VERSION     DAYYNQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.069"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(189..755)
                     /locus_tag="ACHBJ1_002027"
     CDS             complement(189..755)
                     /locus_tag="ACHBJ1_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430950.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(755..1711)
                     /locus_tag="ACHBJ1_002028"
     CDS             complement(755..1711)
                     /locus_tag="ACHBJ1_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM9430951.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1711..2367)
                     /locus_tag="ACHBJ1_002029"
     CDS             complement(1711..2367)
                     /locus_tag="ACHBJ1_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM9430952.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2737..4755
                     /locus_tag="ACHBJ1_002030"
     CDS             2737..4755
                     /locus_tag="ACHBJ1_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9430953.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4825..5031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5182..7596
                     /gene="leuS"
                     /locus_tag="ACHBJ1_002031"
     CDS             5182..7596
                     /gene="leuS"
                     /locus_tag="ACHBJ1_002031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM9430954.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7944..9497
                     /locus_tag="ACHBJ1_002032"
     CDS             7944..9497
                     /locus_tag="ACHBJ1_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM9430955.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9878..11662)
                     /locus_tag="ACHBJ1_002033"
     CDS             complement(9878..11662)
                     /locus_tag="ACHBJ1_002033"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430956.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11677..13398)
                     /locus_tag="ACHBJ1_002034"
     CDS             complement(11677..13398)
                     /locus_tag="ACHBJ1_002034"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430957.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13498..14259
                     /locus_tag="ACHBJ1_002035"
     CDS             13498..14259
                     /locus_tag="ACHBJ1_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM9430958.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4780 a         2711 c         2921 g         4283 t
ORIGIN      
        1 ctgtcctgca tcaagtaata agaacagcca atcaaaaaca gcttctcatg tttttgattt
       61 ggttggactt attaccgcag atataagctt ttgagcaccg tgaataaaga ggttatgaaa
      121 tcaacgctct aacctgagta ttagagaaaa aaacgcaaaa catatcattt atgttttgcg
      181 ttttttattt atcttttttt ctcaatacaa tataaaatag gcggctggtt cttttggttg
      241 ataaaccgat aattcagcac attatatgtt tcctgtggca gtttggaaca aaaagaatca
      301 accatatcca gttctttttc tccaccttca tgaccatagt agacgacaag gatcaatcgt
      361 ccgcgaggca caagtcgttt caatatttct tccatagctg tgcgtgtagt ctctggcata
      421 gtgatgattg ctttatcact ttttgggaga taccctagat tgaaaatccc tgctttgaga
      481 ttttgttgtt cagctatcac ttctcctaac gtttcgtggc cttgtagaaa caatgagaca
      541 cgttcttcta actgacgctg ttctagcctt tcacgagtag catcgattgc ttgttgctgg
      601 atatcaaaac tatagacatg accagttttt ccgatgtgtt ctgctagaaa aaccgtatcg
      661 tggccattgc ccatcgttgc atctacgacg tgatcccctg gttgcaagat ttcttgcagt
      721 aatgtatggc tgaaatgcaa agcagtttgc aacatcagac gctcaccttt ttccgtacat
      781 catattttcc ttggaagcta tctctgcgaa gaagttcttc atcaattgca ttcaagactt
      841 cccatttttt caagctccac attggtccaa tcaaggaatc ccacggcgca tcccctgtca
      901 aacgatggat gatgatctct tttggtatca tttccagctg gtcgcaaatg acattgacat
      961 agtcaggacg gctcattaat tgaaggcgac cttcatgata gtcccgcaac attctcgttt
     1021 tgcgcatcag atgcatcaaa tggagcttga ttccttggat atctgaatct aaaatggtgc
     1081 gtctgacatt ttctttcatc atttctaaac tttcgcctgg taatccgttg atcaaatgcg
     1141 tacaaacacg gatattatgt ttgcgtaatt tagccacgcc atctaaataa gtttgataat
     1201 catgtgctcg gttgatcagt tttgaagttt cttcaaaagt tgtttgcaat ccaagttcta
     1261 cccataaata catccgctcg ttcagttcag caaggtaatc aactacttca tctggcagac
     1321 aatccggtcg tgtaccgact gataatccta cgacacctgg taaattgact acttgctcaa
     1381 atcgttcttt gattatttca actggcgcat gggtgtttgt aaaattttgg aaataaacga
     1441 tgtattgttg gacatgaggc catttttgat gcatcatgtc gatttctttc tggaattgga
     1501 ttggaagagg gtcttcaggt gcaacgatca tgtctcccga accagataca ctgcaaaaag
     1561 tacaaccgcc atgggcaaca gtcccatctc gattcggaca gtcaaatcct ccatcgatcg
     1621 gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc gtaattccaa gaatgatatc
     1681 ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc ttctttctgt aagagttatc
     1741 tatttgtttt cttttttctc tatacactgg ataagtcagg cacagccaag aaagactgag
     1801 taagtaaccg ccgatgatat cagtaggaaa atgaacgccc agataaatgc ggcttatccc
     1861 gacacttact ataataaaca tcatcagcaa ttgaagcaat gtgcgtaagg ttcttttttg
     1921 aacaaaagct ggaagcagaa atagtaaaga accgtaaaga atcatactgc ctgctgcatg
     1981 tccactaggg aaactgtaac ttgtttcaac tactaagtgc gccaatgaag gacgctctcg
     2041 catgaaaaat agtttgatca ccgggttgat gattccagca attacaataa atccagaaac
     2101 gaaccaaaat gtttctgccc tttttttctt ataccacatc cataaagccg cacaagcaaa
     2161 taaaatgatg acggtaagag gattaccgaa tttagtcata aagagttggt agctgttcag
     2221 atcaggataa gaagctctga caatgcctgt aaaccactga tcgaaaccag aaagccacga
     2281 cagatggaac tttacgatcc agcccaaaat gaaaaacagg acaagaaaga agctaccagc
     2341 cacctgaaat gatgttttat atttcataca attcctcctt tgaagcaatg acttattata
     2401 gcatttttgg tccagcgaaa aaaacggtaa agagataaaa ccaatgtatg attcctcttt
     2461 gaataaccaa tattcttata aaaaaaataa ctgtaatcgg aaagacattt tttggtaatg
     2521 caaatgaaat gttagggaaa taaaatcagt gtttttcgaa atccttttaa acaagaacgt
     2581 agacgtagga agaacttgaa aatgaaaaga agttgttcat tacggtaaat tacaatatga
     2641 ttacaaaaaa caacaaagct cttaaataac ttttaataaa tattaagaga agttataatt
     2701 attaataata gataaattga agttggagga tagaagatgg aaaacattgc aagaaaagaa
     2761 cggagacgtt tagaacagac caaacgtttt cgaaaagcaa aacgaggggc agcaatcgtg
     2821 ggaactgcga tggtaggctg ttctgtcgct gccccattag tacagccggt tcctgttcaa
     2881 gcggatgaaa cacctgttca gttcggcgca agaatcaata cagcggcatt tatcacagaa
     2941 attgctactt atgctcaacc aatcgctcaa gccaacgatc tatatgcatc agtgatgatc
     3001 gcacaagcag ttgtagaaag cggttggggc agtagtgcgt tatctcaagc accttattat
     3061 aatctttttg ggataaaagg cagctatcaa ggacaaaccg tctacatgga tacgcttgaa
     3121 tttttaaata atcaatgggt aaataaaaag gagcctttcc gacaatatcc ttcttttgct
     3181 gaatctttca atgacaatgc ctatgtactt agaaatactt catttggcaa cggttattac
     3241 tatgccggta cttggaagag caacacgaaa tcctatacag atgcgacagc ttgtttgaca
     3301 gggcgttatg cgactgatcc tggttacgca ggcaaattaa ataatatcat cgcaacttac
     3361 ggattgaccc aatatgatac accagctagc ggaaacgcag gcggtggagc gactacaggt
     3421 ggcaataata ctggaagtgc aggtagtact ggcggttcaa gcaattctgg taatacaaac
     3481 tcaggttcag caacttcgac cacttatact gtccaatctg gtgattctgt ttggggaatc
     3541 tcaaataaat tcggtatcac aatggatcaa ttgatccaat ggaacaacat ccaaaacaat
     3601 ttcatttatc caggacaaaa actgacaatc aaaggtagcc aggaaaatgg ctcttcgacg
     3661 aataattccg gtaacaatac caattcttca ggaaatgctg gtacatcaaa cggaggacaa
     3721 acaactggtg cgaaatacac tgttcaatct ggtgattctg tttggaagat ctcaaatgat
     3781 cacggaatta cgatggctca attgattgaa tggaacaata tcaagaacaa tttcgtttat
     3841 ccaggacaac agctaatcgt atcaaatggc ggttcttcta atgctggagc aaatactggc
     3901 tcaaatactg ggaatacaag tggctctagt tcgtccagct caagttcttc taatacagct
     3961 tcaggaacga aatatacagt taaagccgga gactctgttt ggagcgtctc aaataaatac
     4021 ggaattacga tggctcagct aatccaatgg aacaatatcc aaaataactt tatctaccca
     4081 ggacaacaac tgactgtttc aaacagtgga tcaacaaaca gcacgtcagg taatacaggt
     4141 tcgtcaacca atacaaataa cagcagttca gttaatactg gcagcagttc gtctcaaggc
     4201 acttataccg tgaaagcagg tgaatctgtt tggagcgtgg caaataaaaa cggcatcacg
     4261 atggatcagt taatcgaatg gaataatatc aaaaacaact tcatttatcc aggacagcaa
     4321 ttgatcgtta aaggtggaac ttcggcaaat acaaatacag gttcaactac ttctgcagcg
     4381 aaaccaaata caccgaatac gtctgctacg acaagtacat catccactgg taatacgatg
     4441 tataccgtga aagcgggcga gtcagtttgg agcgtagcga acaaacatca tatcacgatg
     4501 gatcagttaa tcgaatggaa taatatcaaa aataatttca tctatccagg acagcaagtg
     4561 attgtgaaga aaggttcatc tcaatcaaca aaccaacaat cagttactgg ttcgaaaacc
     4621 tatactgtga aatctggaga atctgtttgg agtgttgctg acagtcatgg aattacaatg
     4681 gctcaactaa tcgaatggaa caatattaag aacaacttta tttatccagg tcaaacgttg
     4741 attgtagcta aataaaatag ctgttcgaag aaataacgat tgaattccac aaaaatattt
     4801 gttactataa ggctacttaa atatgacaac acttgaaaag gaagtagtaa aaagactgaa
     4861 gtttcagaga gcgcatggct ggtgagaatg tgcacggatg cttttgaact cgcctttgag
     4921 ttgcccttgc tgaatacccg agtaagcaag cggcggtgac ggccgttaca acgttcgagg
     4981 ccttttcact aaggtaaaat taggtggtac cgcgtagatt acgtcctata aaaagagcaa
     5041 tctttttata ggattttttt atttcttttg tgaagtttga gaaaacaaag caaaaggaaa
     5101 taaaaataaa cagattctgc cggttttttc gttatattta taatagaaac aagtgttttc
     5161 aatagaacag gaggacagca aatgagttac aatcataaag aaattgagaa aaaatggcaa
     5221 aaatactggg caaaaacgaa tactttcaac acccatgatg atcctgaaaa gccaaaattt
     5281 tacgcactgg atatgtttcc ctatccatcc ggtcaagggc tacatgtagg gcatcctgaa
     5341 ggttatacgg caacggatat cctttcacgt gtgaaaagaa gtcaaggata caacgtgctg
     5401 catccaatgg ggtgggatgc attcggtctg cctgctgaac aatatgcgct agatacagga
     5461 aacgatccag ctgagtttac ccaaaaaaat atcgaaacat tccgacgcca aatcaattct
     5521 ttaggattta gttatgactg gaatcgcgaa gtgaatacaa ctgatccgga gtattataaa
     5581 tggacacaat ggatctttac gaagctttat gaaaaaggtc tagcttatga agcagaagta
     5641 gcggtaaact gggtacctga attaggaaca gttatttcaa atgaagaagt gatcgatggg
     5701 aaaagtgaac gtggtggcta tgatgtagtc agaaaaccaa tgcgccaatg gatgctgaaa
     5761 atcactgctt atgctgatcg cttattggat gatctagaat tagttgattg gccagaaagc
     5821 attaaagaaa tgcaaagaaa ttggatcggt cgttcagtag gagcaaatat tgaatttaaa
     5881 gtggctggta cggataaatc ctataccgta tttacgacac gaccagatac tctattcggt
     5941 gcaacttact cggttcttgc acctgaacta gatcttgtta gagaaattac gacccctgaa
     6001 caaaaagctg ctgtagaagc ttatatcgaa gagacagcta aaaaatcaga tctaaagaga
     6061 acggatcttg caaaagaaaa aacaggagta tttactggtg cttatgcaat caatccagtt
     6121 aatggcaagg aaatcccaat ctggatcgcc gattatgtat tatcttcata cggcacagga
     6181 gccatcatgg ctgttccagc acatgatgaa cgtgactttg aattcgctca aactttcgga
     6241 ctagaaattc ttccagttat cgaaggtgga gatgttgaaa aagctgctta cacagaagat
     6301 ggtacgcaca tcaactctga atttctagac ggcatgaaca aacaagaagc aatcgataaa
     6361 atgaatgaat ggctagaaga aaatggcgta gggaaaaaag aagtcagcta tcgcttacgt
     6421 gactggctat tctcccgtca acgttactgg ggtgaaccaa tcccaatcat ccattgggag
     6481 gacggaacag tcacagctgt cccagaagaa gagctaccat tacggttgcc taagacaaaa
     6541 aacatcaagc caagcggtac tggagaatct ccattagcaa atatcgagga gtgggtcaat
     6601 gtcgttgatc ctgtaacagg taaaaaaggc cgtcgcgaaa cgaatacaat gccacaatgg
     6661 gcaggaagct catggtatta cctgcgttat atcgaccctc ataacaaaaa agaactagca
     6721 gattatgaaa agttgaagcg ttggctacca gttgacatct acattggtgg agcagaacat
     6781 gcggtacttc atttactata tgcacgattc tggcataaat tcttatacga tatcggtgtt
     6841 gttcctacaa aagaaccatt ccaaaaacta tacaatcaag gaatgatctt aggcgaaaac
     6901 aacgaaaaaa tgtcaaaatc tcgtggtaat gtagtcaatc ctgatgatgt ggtcgaaact
     6961 tacggtgcag atacactgcg catgtacgaa atgttcatgg ggccattaga tgcttcgatt
     7021 gcttggagcg aaaatggctt agaaggaagc cgtaaattct tggatcgtgt atggcgtttg
     7081 atcgttgatg agaacaataa aatgcgtgac cgtatcacta cattgaatga tggcaagctt
     7141 gataaagtgt atcatcaaac agtgaaaaaa gtgacagaag actatgaaaa tcttcatttc
     7201 aatacagcaa tctcccaatt gatggtcttt atcaatgaag cctataaagt ggacgctctg
     7261 ccttatgaat atatcgaagg atttgttcaa ctactcgcac cgattgctcc tcacatgggt
     7321 gaagaactat gggcaatcct tggaaatgat ggtggtattt cttacgcacc atggccaaca
     7381 tacgatgaag cagcacttgt tgaagacgaa gtagaagttg ttttccaagt aaacggtaaa
     7441 gttcgtgcga aaagcaatgt tccccgagac ttaggaaaag atgagctaga aaaagcagca
     7501 ttagccaatg aaatagtgca agaatatatt gaaggcaaaa cagtcagaaa agttattgcg
     7561 gtaccaaaca agttagtcaa tatcgttgct aattaattgg ctctttgtca ataaggactg
     7621 atgagttgtg caaaatcaaa tctgggccag aatgaacttc attctgtctc agattttttg
     7681 ttatttcaaa ttgattaatt gattgatcaa aaaggttttg attttcatgt tctcaaacac
     7741 gttcaatgtc tttttcttct gaatggtctt gtaagagatc tctgtaatca tcaaattttt
     7801 gtctattaat cttagtattt tttgatgtat aaactacctg aaattgagag tacataccaa
     7861 attcataaat tttttattag tgggactagt gattgtatgg attttgacgc ataatgaatg
     7921 aagtatgaga aaaggagaga caaatggatc aagaactatt tattatcgaa gtattatcag
     7981 ctaaacaaaa aattatcgag aaagcgaaaa aaagagcgaa aagaataatg ttcaagcttt
     8041 taagcttagt atttttctct ggtttgcttt ttttcggagc tgtatcgttc ctatatcgcc
     8101 atatgagtgc agtagtccgt tcagctgaag ggatgcaaaa attaaataat ttgtatcact
     8161 tttcgtgggg agttggcgga ataacaattg ttgtgcttat tgtttttctt ctacttattt
     8221 ggctccgtta ccatcaattg tcgcgtcatt tcgatcaatg gcttagtcaa agagcacaac
     8281 agtttttaca taataaacca acttctcaag atgatgacta tctttatttt gagaagactt
     8341 cgacgagtca agcttttcgg atacaaaaaa gtgattgcca gttattgctg agatcacttg
     8401 atgatttacc tatctactta ggcgaaagca aatcaaaatt cggaatgaac acattccata
     8461 tttttttact agaaaacgaa cccagatggg aaaaaagcca gtcttcgcct ggtggatacc
     8521 tgacaactaa gatgagtaca ttagtcactg gttttttcct cattctttta gctggaacaa
     8581 tctattatgg gagatacact cagccaaaaa tgtcttctca tataaatgct gcttctcaca
     8641 tgacaatgaa ctcaactaac gatacgcata tacaaaagac aaatgaaggg ttgttagctg
     8701 atcctaagca aaaggtaaat ttgacaacac aaaagataaa tgatctcaaa cttgatacag
     8761 agacgcactc tctctatatg acaacagata gtggtgctaa ttgggtattt gtgccgattg
     8821 attcgcaatg gttgcgtttt ggcgaataca ccctgacaac ggggctgatt cctgaaggtt
     8881 attggatgga caacacatat gatatttccg ccgatttctc atggtttatc tattcttctg
     8941 gggatgatgt acgtctgctg acttcaagag ataatggtaa aacatggcaa acgtctatta
     9001 tcacccaatc agttcagccg atacgttatc gaaaagcggt attttatggc aacgatggtc
     9061 tatttatctg tacatcagcc agtgggatgt ctgcagaaac tttgtacatt tacactacta
     9121 tggatggcgg aatcacttgg ataaaaagtg gaagtacaat gatcaatcag ccagtacaaa
     9181 atgtcagctt cgttacaact tctttaggtt ttgtttcgac acgtgaaagt ttgttttata
     9241 caaacaacgg aggacgctca ttcaaagaat cagttctgtc gattccggaa aactatcaga
     9301 caggggggat agatcttttt caatcaccaa atgaagttac tcaaataagc gcgaatacgt
     9361 tagagacaaa attttatttg ttgaaaaaag gaccaatcga tgcagggaaa atgtttgcct
     9421 gcctcttccg ttcaactgat aacggggaaa catggcaatt cgttcagcaa ctatcgcaag
     9481 tagaacaagg agattaaaga agagagattg tgagcctgac gttcagcacc gagtcacaag
     9541 aaacaaattc aaaaaacagc ttctcatatt tgttgaatag tgtaactcgt gattcggagt
     9601 tgacaagagc aacaagaagc gattttgaag tatagcttct cgtattttac aagatcgtgt
     9661 cttattgccg ataggttgtc tcttgaacac cgtttattcg taaaaaggga ttgtgacaag
     9721 tcttttgtca ctacttaccg aacgaaactg aaggagctat ccaaaaagta tcattagtat
     9781 tattctttgt gtttctagtg gcaccactag tacatttaaa aaagctatcg atcatggact
     9841 aatcatacat gatcgatagc tttttagata atgtttgtta cgctatctga aattgactat
     9901 tataaagact ggcataaagc gttggtgatt ggagtagttc agaatgtgtt cctttttcga
     9961 cgatatcgcc attcttcatt acaaggatca tgtctgcatt ttcaatcgtt gacaaacggt
    10021 gtgcaatcac aaaacttgtc ctatcttttg tcacagcttc cattgcttgc tgaatcaatg
    10081 cttctgttcg tgtatctacg ctagaagttg cttcatctaa aataacgaca ggaggattcg
    10141 ctagaatgac tcgagcaatt gtcaataatt gttgctgtcc ttgagataac gtgccgtttt
    10201 cgctagaaat gatcgtatca tatccgtctg gcagtgttcg aataaagtga tcacattgtg
    10261 cgatttcagc cgcttcgata atttgttctc ttgtggcatc cattttcccg taggcgatat
    10321 tatcagctac cgttccttca aacagccagg tattttgtaa aaccatccca aaagaacgac
    10381 gtagttctcc acgtgaaagc ttggtaatat caagaccgtc gaattcgatc tttcctttat
    10441 tcagttcata aaaacgcatc agcagattga ctaaagtcgt ttttcctgca ccagttggac
    10501 caacgatcgc taccgtttgt tttggcttta ctgaaaaatc gacttgattc atcaatattt
    10561 tatctgatgt gtaaccaaac tgtacatttt tgaaagctat ttttcctttc ggttgttcta
    10621 aaagtcttgc atctggaagg tctggaattt catctgtttc atctaatatc tcaaatatcc
    10681 tatcgattgc tgccattgcg gattggatcg agttgatcac ataagaagcg gtagaaattg
    10741 gttcagaaat ctgattgatg tactgaagat aagcttgtaa gaagccgatc gtcacaccac
    10801 ctgatagaac aagcatagca ccgaataccg cacttatgat gaaggcgagt tgattaatga
    10861 aacgaatggc cggatagata gcacagttca taaattgagc acgtttaaaa gcgttgtatt
    10921 gctttttatt tacttcgtgt atagcagctt ctgcatgagc ttgctgattg aatgttttga
    10981 ccactagatt accagctaag atttcttcca ttcgattatt taaaacaccc agttcatact
    11041 gattctgttc agaaatctgt ttgtttttgt tcgcaatctt agttgtaaca attgaactgc
    11101 caacgatcag caataagacg agcaaagtaa gcttgctttc gatataaaac agcatcagac
    11161 cagcagcagc tattgttacg acagaagtaa aaaattgatt gatacctgtc aataacactt
    11221 gagaaagttg attgagaccg gtagttgtcc gactaatgat gtcacctact tgatgattgt
    11281 caaaaaacga catcggtaat gttttgaatt tttttgtgat ttcttttcgc accctcaatg
    11341 tgatcttttc gcttaacgct gccattgtcc gttcctgaat aaatgaagtg atgctgctta
    11401 agactgaaca gccgatcagc aataagacag ggaacaacaa tgcttgtttg atatcactga
    11461 aagaaagctg tccaatacct gtttttctta tttctgataa gagatcatcg atcccgattc
    11521 ccatgatcaa gggcatggct acgactaata ggttcccgaa taaactgcat agaaacaatc
    11581 cgtaaaaata tttatgttca ggtttgatca ttttccaaaa acgtatcaaa gcacgcgtgt
    11641 tcaacgtttt tttcttttcc atcgtatccc ttctttctat attccttgtg attttgcaaa
    11701 ctcctgataa gtttggcaat tagacagtag ttcttcatga gtaccttgtc caacgatttt
    11761 tccttcatct aaaacaatga tgttatctgc ttgttggatt gtactgaggc gttgagcaac
    11821 aatcacgaga gttttctccg acatgtgttt atgaagggct gctcttaatg ctgcatctgt
    11881 tttatagtct aaagcggaga aactatcatc gaatacatag atctctgctg gtttgatcaa
    11941 cgctcgagca atacatatcc gctgtttctg gccccctgaa taattagaac cgccttgagc
    12001 aacaaagctg tccaatccgt caggaagggt gtccactagt tcttctaatt gagcgatctt
    12061 gatcgcttta gacaactctt ctgttgaggc atattcattt cccatctgca agttgcttaa
    12121 aattgtcccg ctgaataaga aagccttctg agggacataa ctgatgtggg agcggatctg
    12181 ttcttgagtc atttgagtaa gaggaatacc accaagggtt acttttccta tcgtagggtc
    12241 actaagtctc aagagaagtt tcgccaaact actttttcca gatccggtcc cgccaacgat
    12301 cgctgttgtt tttcctttag gtattgtgaa atcaatatct tctagaactg gatcagagat
    12361 ttcagtatat ttaaatgtga catgttcaaa agcagctgca tttgtctgat caaaggttgg
    12421 ttcagctact gttttgatcg tctcttcctc aatttcgatc tctgcttgca gtatttcttc
    12481 tatccgtcgt aatgaggcaa acgatttagg gataatgacc aataccatag ctgcgatcat
    12541 caagtaagca agtgtcagca tagagtattc gactacagca gtcacgacac cgatttgtaa
    12601 tgctccttct gctacaagat ttccgccaaa ccacaaaata gaagcataaa cgattcccat
    12661 gagcaaaaaa gcaattggag tgacccaagc aaatattcgg ttcactttga tcatttgatt
    12721 agcatagtca gcaaagctct tattcgttcg cttttcttca tatgattgat tatcaaaggc
    12781 acggatcata ttaatcccag taaagaattc tctcaatgtg atggtgatcc gatccatttt
    12841 tggctggata ctcaaagata aaggtgcccc tcgtttcatc agtcgataaa caatccatac
    12901 ataaaaaaca acagagatca atgtaaccca tgttagtaat agagaataac gaaacatcat
    12961 gagaatggtg aaaacgcaaa taaccggtgc tggtaagatc atttggcaaa ataaaacaat
    13021 catttgctgg acattatcga catcattcgt cattcttgtc aaaagagatc cagaactaaa
    13081 acgactgata tccttcattg acagctgttg gaatttacgg aaatatttct cccgcgtttg
    13141 gaatccaaac ctagcggaag ccaatgctga aaagtaacta gaggcaactg cagcgattgt
    13201 tcctagaaac gctacaagca gcatttcaac tccaattttt tttataactg aggcttcgcc
    13261 acttgctata cctgtgtcga tcagctttgc tactaatttt ggtacaccta atgtaccggc
    13321 gatttctaaa cagatcatca caaaggtaag gagagcgagt cctttgtttt tctgtaagaa
    13381 tgtttttaag atagacatat tattcctcct tttttcctga tacttttcga ttctatacct
    13441 tcaagtaact tgaaggtcaa tagaaaaaag aataaactga aaaagaggtg agaaaaaatg
    13501 aaaaaaatgt atacgattgg ccaagtatcg gaactatttg atttgccaaa atcaacgatc
    13561 cgctattggg atgaacaagg attaatccgt tcaagtagac aagaagagaa tgattatcga
    13621 ttgtttgata tcgatgatat ttttatgctt tatgatatcg ttttttatag aaaattagat
    13681 ataccgatca aacagatgaa gaatttatat ggaaaaacat tgacggagtt atatgagaca
    13741 cttgatgaaa cagaaagaag aatccataaa gaactggtgg tgatgaaaca aaaacaaaaa
    13801 gagataaggg agcaaaaaaa acagttgaaa ttgatgatcg atacaaacga ggaagaattt
    13861 cctgtagaag agatcccttt tgactgtatg atctcaacgg agtttgaaga tattgtggaa
    13921 atcaaaaaat ttctacctaa ctattcaagc tttgggatga tgagtatgcc agcttccagt
    13981 tcacagacga tgtatggatt ttttatcgac cctagtgaag tacatttatt tactcaagac
    14041 gtgatttggg agaaaaaaga tacggctgtt tatcgtcgat tcttattgaa atccgagatg
    14101 aaccatgcgg aacgtaataa tattttagaa atcagagaaa gaatgtatga aaaaggatgg
    14161 aaaacaggag aagtgatcgg ccagtatcta ttgacgaata cggatgaaaa caatatcaga
    14221 actgaatatt atcatgcatg gatagaaatg aaaaaatagg aaatatgtgt ttaagataac
    14281 tcgaaaatga tggttgtttt agttataaag aaaaattttc tggttcgatt acgggtttaa
    14341 tgtgggggat tattagtcgt ggatagatag aattttagtt ttttagagga aatatgtctt
    14401 taaaaatgga atggttgcaa ttcttagcga tatatacgat gtaattgtcc tcttaaaaaa
    14461 gtatggaaaa ataattgtat tgggtgttat gtataattca tggtgaactg agactaatga
    14521 gaagaaaaat tcatattgtt atgagtgaga ttgtatcaga tgagaatgat aaattaaata
    14581 ggtgcataaa agcattacac ttaaaataca ttgttgctta cattattaca atatgttaat
    14641 aaggcaaaaa taaatagatg gattgaataa acatattttt taatcaaata gtccc
//
LOCUS       DAYYNQ010000070        14256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000070 DAYYNQ010000000
VERSION     DAYYNQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.070"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(432..1616)
                     /locus_tag="ACHBJ1_002036"
     CDS             complement(432..1616)
                     /locus_tag="ACHBJ1_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM9430959.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1641..2648)
                     /locus_tag="ACHBJ1_002037"
     CDS             complement(1641..2648)
                     /locus_tag="ACHBJ1_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9430960.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2820..3179)
                     /gene="comGG"
                     /locus_tag="ACHBJ1_002038"
     CDS             complement(2820..3179)
                     /gene="comGG"
                     /locus_tag="ACHBJ1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM9430961.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3151..3576)
                     /gene="comGF"
                     /locus_tag="ACHBJ1_002039"
     CDS             complement(3151..3576)
                     /gene="comGF"
                     /locus_tag="ACHBJ1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM9430962.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3539..3886)
                     /locus_tag="ACHBJ1_002040"
     CDS             complement(3539..3886)
                     /locus_tag="ACHBJ1_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430963.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(3858..4328)
                     /gene="comGD"
                     /locus_tag="ACHBJ1_002041"
     CDS             complement(3858..4328)
                     /gene="comGD"
                     /locus_tag="ACHBJ1_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM9430964.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4303..4581)
                     /gene="comGC"
                     /locus_tag="ACHBJ1_002042"
     CDS             complement(4303..4581)
                     /gene="comGC"
                     /locus_tag="ACHBJ1_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM9430965.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4578..5627)
                     /gene="comGB"
                     /locus_tag="ACHBJ1_002043"
     CDS             complement(4578..5627)
                     /gene="comGB"
                     /locus_tag="ACHBJ1_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM9430966.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5584..6513)
                     /gene="comGA"
                     /locus_tag="ACHBJ1_002044"
     CDS             complement(5584..6513)
                     /gene="comGA"
                     /locus_tag="ACHBJ1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM9430967.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6645..8399
                     /gene="ade"
                     /locus_tag="ACHBJ1_002045"
     CDS             6645..8399
                     /gene="ade"
                     /locus_tag="ACHBJ1_002045"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM9430968.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8497..9159)
                     /gene="trmB"
                     /locus_tag="ACHBJ1_002046"
     CDS             complement(8497..9159)
                     /gene="trmB"
                     /locus_tag="ACHBJ1_002046"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM9430969.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9229..10011)
                     /locus_tag="ACHBJ1_002047"
     CDS             complement(9229..10011)
                     /locus_tag="ACHBJ1_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM9430970.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10215..11426)
                     /locus_tag="ACHBJ1_002048"
     CDS             complement(10215..11426)
                     /locus_tag="ACHBJ1_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430971.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11419..12156)
                     /locus_tag="ACHBJ1_002049"
     CDS             complement(11419..12156)
                     /locus_tag="ACHBJ1_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430972.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12294..12728
                     /locus_tag="ACHBJ1_002050"
     CDS             12294..12728
                     /locus_tag="ACHBJ1_002050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM9430973.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12721..13065
                     /locus_tag="ACHBJ1_002051"
     CDS             12721..13065
                     /locus_tag="ACHBJ1_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM9430974.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13245..14255
                     /locus_tag="ACHBJ1_002052"
     CDS             13245..14255
                     /locus_tag="ACHBJ1_002052"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9430975.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4533 a         2910 c         2401 g         4412 t
ORIGIN      
        1 tttttcgcaa caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca
       61 ggtggaaaat caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta
      121 aacataaagc gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt
      181 tttacatatg taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga
      241 aaatcaataa ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc
      301 taagaagatc ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct
      361 ccgcaaaaaa aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg
      421 aatgctttgg cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct
      481 gttgggatca ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca
      541 cgcaattcat tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt
      601 tcactacgta catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca
      661 ccattcatcg ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca
      721 taagctaaac gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac
      781 aatcctgtta ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata
      841 tcaatctcta atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt
      901 gtacccattg tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct
      961 ccatcgactg ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct
     1021 tcgatcggtt tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca
     1081 tggaagccat attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa
     1141 taattttgct ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg
     1201 tcaggtaaaa tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga
     1261 gcaaattctg ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc
     1321 ccataagttt ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag
     1381 cttaaaattt gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt
     1441 tcgatatcca tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa
     1501 tcttgtaaac cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga
     1561 tataattgcc atttcaaact tgaactccca gcattgattg caattgtttt agacatatga
     1621 ctctccttct cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa
     1681 aattccgtca ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt
     1741 acttgttgtg cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta
     1801 ttacggaaca attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac
     1861 cattttttga tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa
     1921 ccaaaaccat ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa
     1981 ctatggcctt cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa
     2041 ccgattggca aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg
     2101 tcttgatgaa agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc
     2161 aacaatgtat catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg
     2221 tttaaaagaa ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc
     2281 ggtgcttttt tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta
     2341 tcaggagtga gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt
     2401 aattgagaca gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa
     2461 agagctgtga tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga
     2521 taatcatcta ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat
     2581 gcattctgta aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct
     2641 ggtaacatgg tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc
     2701 tatcgaacaa ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa
     2761 cattttttta ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat
     2821 cattctgtac tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt
     2881 gagacttcca cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt
     2941 gtgtagtaaa aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc
     3001 tgcttcatta tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa
     3061 cggtagcttt ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca
     3121 gaaaataaaa tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata
     3181 tccatcttct ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa
     3241 ttgaagctca gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg
     3301 tatcattgcc tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg
     3361 ctgatttttc acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa
     3421 tattagccac tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg
     3481 aatcaaaaga ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc
     3541 aatcagcgta aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat
     3601 tgacaaacac tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca
     3661 tttcttccct tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa
     3721 tcattcgaat aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct
     3781 ggccaaagct cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca
     3841 acaaaacaaa tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat
     3901 tgatacgtaa tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt
     3961 tcattcccgg ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca
     4021 ggaatatcta atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga
     4081 ttctcttcac tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc
     4141 gtagaataga ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc
     4201 catgcagaaa atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag
     4261 agtgattcaa acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat
     4321 ttctgcatgt tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct
     4381 tccattattt tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc
     4441 acgactttct tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac
     4501 taatacactg atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata
     4561 agatattttt ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa
     4621 acagcggcat aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata
     4681 aaatgcatcc agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa
     4741 atcagttctt tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct
     4801 tctgatagaa aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct
     4861 tgaatcaact tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag
     4921 actatgattt gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa
     4981 taagccgatt gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca
     5041 ctctgatcaa caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta
     5101 accgctagga tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa
     5161 tgctcttgca tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta
     5221 aacaagatac ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa
     5281 tcattgcgaa atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc
     5341 agattcccat gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt
     5401 tgcgaaagta cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa
     5461 ataacaggtg gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa
     5521 ccagataaaa gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat
     5581 cctttagaat agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca
     5641 tgattttttt actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg
     5701 attggcatct tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc
     5761 ttttttcttt gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac
     5821 ggttgcaaaa acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc
     5881 atctcttatc tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag
     5941 tgtatcataa gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc
     6001 tatctctaca ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata
     6061 cattagagaa gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac
     6121 ggttttctgt aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa
     6181 agtaccgtat aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga
     6241 caaccgcaga cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt
     6301 tcttttttct ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc
     6361 atctgccgaa gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta
     6421 aaatacttct gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact
     6481 tatcatttga tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg
     6541 tctttttgat ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg
     6601 aatttatgtt atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa
     6661 ggaaatatat cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta
     6721 agatcgtcaa cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa
     6781 tgatcgtagg aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc
     6841 tagtgccagg attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg
     6901 aattttcaaa agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa
     6961 taggaaacat tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac
     7021 tcatgaatat cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg
     7081 gtgcaaattt aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct
     7141 tagctgaagt catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga
     7201 tccaattgat gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg
     7261 aagagttaaa tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa
     7321 aagaagctaa agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg
     7381 caaaagatct ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct
     7441 ttgtcaccga tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg
     7501 tccgtcaagc tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga
     7561 atgcagcaga atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg
     7621 atttcttctt aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa
     7681 cattagttgt tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat
     7741 atacgtgttg tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc
     7801 ttctttcttc ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag
     7861 atttggtaga aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc
     7921 aattaaagat tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag
     7981 tgaaaggatt tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata
     8041 atctagttgt cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc
     8101 aaaaaggtgg cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc
     8161 ctataggcgg aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat
     8221 taataactaa agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt
     8281 ctttcttaac attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat
     8341 tttctcgctt ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa
     8401 tcaaataaat cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat
     8461 ggatattaat gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt
     8521 atcttgaaat tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc
     8581 atactccgtc ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt
     8641 gaggatcatg ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc
     8701 tgttttgaaa tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt
     8761 cttatatgtc aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag
     8821 atagatttga tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg
     8881 tagaagtttc aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac
     8941 actgacctgc atttctactc caatataatt gatttcagga tgggcttttg ccattccagt
     9001 gatgaactgg cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa
     9061 ttcattccat tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc
     9121 taacatttcc gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc
     9181 tctaattaaa aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa
     9241 aattcgtttt aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata
     9301 tctggtgatt tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc
     9361 gtcagtcggg cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata
     9421 atttcctaaa atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc
     9481 gactagatat aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac
     9541 tactgataag ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat
     9601 ttgcagaaat tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat
     9661 cttctccggc gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga
     9721 atgatgtaaa tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt
     9781 cagcaagcga ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt
     9841 tgtccaaaca agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt
     9901 gtttcgttta ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc
     9961 gcctttgatg ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc
    10021 cgtcctctta ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc
    10081 aagcacatat acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa
    10141 aaatagaacg aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct
    10201 acacagcttt gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca
    10261 aagagcatag taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat
    10321 acgactgcag caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga
    10381 tactgaatcg cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata
    10441 tagcggaact ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa
    10501 ccgataatca gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt
    10561 actaatcgga aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag
    10621 taaagataag tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt
    10681 cttcttttga tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag
    10741 atctgatgca acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct
    10801 tgttgcttcc acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct
    10861 gcccatatta gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct
    10921 aatttccaat aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc
    10981 agatgactga atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat
    11041 tgctccgtac taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc
    11101 aacggaaaaa tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct
    11161 ttaggaagta aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat
    11221 aaacagagca tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg
    11281 gttttcaacc aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa
    11341 acaaaatgat cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct
    11401 aaacgttttt gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc
    11461 gtccaaagaa gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc
    11521 tcgtatctct ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa
    11581 aatatgcgta gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag
    11641 caatgcattg atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga
    11701 aggctcgatc aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt
    11761 agctgggaac cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct
    11821 tttcattgct atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct
    11881 caatgtcaat tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata
    11941 ttcttccgta tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc
    12001 cagcagaccg ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa
    12061 ccctactatc tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc
    12121 gtacccccct gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt
    12181 tatttattat accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa
    12241 cttatggtaa agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa
    12301 actgtatttt ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg
    12361 acaaggtcta tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc
    12421 ctaaacgtca tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt
    12481 cgcgtgtgcc taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta
    12541 atatcttgaa taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt
    12601 tagtccctcg ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata
    12661 gttatggatc ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg
    12721 atgattaaaa actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt
    12781 ctgttattgg cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa
    12841 gaagcaacaa ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg
    12901 atcgaaacaa aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa
    12961 gatatcgaag attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta
    13021 gaaaaactaa aagctgatct gccagagtat cctgaacaaa tataataatg attattttct
    13081 caacagattt aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat
    13141 tttgaatttt gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag
    13201 ttaatatata aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa
    13261 ttattttggc agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata
    13321 ctaataaaga tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg
    13381 aagcaaaact tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat
    13441 tcaataacaa atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac
    13501 aagcgaaatc attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag
    13561 atacttataa agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg
    13621 tagatattac agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag
    13681 cacaaatcat caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg
    13741 atggagatga tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag
    13801 atggcggtaa agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag
    13861 cagctttcaa attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt
    13921 cttatgcgac agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca
    13981 tggataaata caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg
    14041 cattcacaac taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg
    14101 atgcctttga aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa
    14161 gtgaaactac agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat
    14221 cttcaactga agaaacaact gattcatcaa aataaa
//
LOCUS       DAYYNQ010000071        14130 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000071 DAYYNQ010000000
VERSION     DAYYNQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.071"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHBJ1_002053"
     CDS             complement(<1..244)
                     /locus_tag="ACHBJ1_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9430976.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHBJ1_002054"
     CDS             complement(237..1829)
                     /locus_tag="ACHBJ1_002054"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM9430977.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="ACHBJ1_002055"
     CDS             complement(2339..3064)
                     /locus_tag="ACHBJ1_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430978.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="ACHBJ1_002056"
     CDS             complement(3218..3544)
                     /locus_tag="ACHBJ1_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430979.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="ACHBJ1_002057"
     CDS             complement(3581..3946)
                     /locus_tag="ACHBJ1_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM9430980.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="ACHBJ1_002058"
     CDS             complement(3983..4198)
                     /locus_tag="ACHBJ1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430981.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="ACHBJ1_002059"
     CDS             4327..4662
                     /locus_tag="ACHBJ1_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430982.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="ACHBJ1_002060"
     CDS             complement(4738..6735)
                     /locus_tag="ACHBJ1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430983.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="ACHBJ1_002061"
     CDS             complement(6833..7108)
                     /locus_tag="ACHBJ1_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430984.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="ACHBJ1_002062"
     CDS             7340..8074
                     /locus_tag="ACHBJ1_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9430985.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="ACHBJ1_002063"
     CDS             8113..9255
                     /locus_tag="ACHBJ1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430986.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9883..10368)
                     /locus_tag="ACHBJ1_002064"
     CDS             complement(9883..10368)
                     /locus_tag="ACHBJ1_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM9430987.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10370..11041)
                     /locus_tag="ACHBJ1_002065"
     CDS             complement(10370..11041)
                     /locus_tag="ACHBJ1_002065"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9430988.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(11304..12497)
                     /locus_tag="ACHBJ1_002066"
     CDS             complement(11304..12497)
                     /locus_tag="ACHBJ1_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9430989.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(12581..12781)
                     /locus_tag="ACHBJ1_002067"
     CDS             complement(12581..12781)
                     /locus_tag="ACHBJ1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM9430990.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(13205..13438)
                     /locus_tag="ACHBJ1_002068"
     CDS             complement(13205..13438)
                     /locus_tag="ACHBJ1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9430991.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13445..13876)
                     /locus_tag="ACHBJ1_002069"
     CDS             complement(13445..13876)
                     /locus_tag="ACHBJ1_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9430992.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4295 a         2895 c         2270 g         4670 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgttttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca
     9781 gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt
     9841 aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac cttttttgta
     9901 tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata
     9961 ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat
    10021 tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt
    10081 aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc
    10141 tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt
    10201 atctatccag ttttttatag caatatctcc attacgttta actttctcaa attcagcctt
    10261 atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc
    10321 tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct
    10381 gaatactatt tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat
    10441 ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat
    10501 tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta
    10561 ctatattttc attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta
    10621 tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa
    10681 tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa
    10741 gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa
    10801 tactattacc aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc
    10861 tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat
    10921 caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca
    10981 aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca
    11041 aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa
    11101 aatcctcaga atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt
    11161 agaagttctt gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt
    11221 gttagatact tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat
    11281 ggagtagtaa aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa
    11341 tcgaatgtgg cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg
    11401 tactgcaagg ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc
    11461 atagcatggg gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc
    11521 acaagcctgc caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg
    11581 agaaacagga aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt
    11641 atcactcgct ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt
    11701 tgggtcttgg gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc
    11761 acgatgatta cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa
    11821 atgcgaagcc ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta
    11881 tcaccttgaa taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt
    11941 tccgtatcgt cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa
    12001 tcgtcctgta tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc
    12061 ttataagaaa ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc
    12121 acactgtcaa tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc
    12181 cggcccagtt tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc
    12241 tggcagacgg tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt
    12301 tctttctcct tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta
    12361 ggcactaact tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa
    12421 gcatatcgtc cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct
    12481 cgtctttttt ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc
    12541 ttataaatta ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt
    12601 caattatttt ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc
    12661 agccagaggc cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat
    12721 atttagacgc ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca
    12781 taatgcagcc ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg
    12841 ctgctggcac agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat
    12901 tgcctgcgac gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg
    12961 tacgtcgtcg cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt
    13021 ttcccgtagg gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg
    13081 tccgtatgcc ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca
    13141 ggccaccggg aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg
    13201 ttcgtcagtt cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct
    13261 cgtccagaca cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt
    13321 tcatgtaacc gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg
    13381 cggcctcgat caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc
    13441 gcgtctaacc tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat
    13501 ttgtcctgtg ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat
    13561 ctgccatatc cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg
    13621 caaaggcttc ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg
    13681 taaagctgtc gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca
    13741 aagagcttag tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg
    13801 cctcgttacg cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc
    13861 gggggatagc atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct
    13921 ttccgctaac aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa
    13981 atttctcaaa aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg
    14041 caggtcgcaa acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata
    14101 gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DAYYNQ010000072        14129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000072 DAYYNQ010000000
VERSION     DAYYNQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.072"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBJ1_002070"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHBJ1_002070"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM9430993.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBJ1_002071"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHBJ1_002071"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM9430994.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHBJ1_002072"
     CDS             8118..9086
                     /locus_tag="ACHBJ1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9430995.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHBJ1_002073"
     CDS             9104..10009
                     /locus_tag="ACHBJ1_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9430996.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHBJ1_002074"
     CDS             10006..10812
                     /locus_tag="ACHBJ1_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9430997.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHBJ1_002075"
     CDS             10969..11937
                     /locus_tag="ACHBJ1_002075"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM9430998.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHBJ1_002076"
     CDS             complement(12254..12778)
                     /locus_tag="ACHBJ1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM9430999.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..>14129
                     /gene="scm"
                     /locus_tag="ACHBJ1_002077"
     CDS             13065..>14129
                     /gene="scm"
                     /locus_tag="ACHBJ1_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9431000.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3996 a         3046 c         2791 g         4296 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatca
//
LOCUS       DAYYNQ010000073        13944 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000073 DAYYNQ010000000
VERSION     DAYYNQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.073"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACHBJ1_002078"
     CDS             complement(146..1111)
                     /locus_tag="ACHBJ1_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM9431001.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACHBJ1_002079"
     CDS             1345..2190
                     /locus_tag="ACHBJ1_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9431002.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACHBJ1_002080"
     CDS             2381..3568
                     /locus_tag="ACHBJ1_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM9431003.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACHBJ1_002081"
     CDS             3574..3930
                     /locus_tag="ACHBJ1_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM9431004.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACHBJ1_002082"
     CDS             3932..4270
                     /locus_tag="ACHBJ1_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM9431005.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACHBJ1_002083"
     CDS             4665..5699
                     /locus_tag="ACHBJ1_002083"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM9431006.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACHBJ1_002084"
     CDS             5692..6378
                     /locus_tag="ACHBJ1_002084"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM9431007.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACHBJ1_002085"
     CDS             6402..7250
                     /locus_tag="ACHBJ1_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM9431008.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACHBJ1_002086"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACHBJ1_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM9431009.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACHBJ1_002087"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACHBJ1_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM9431010.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACHBJ1_002088"
     CDS             9516..10751
                     /locus_tag="ACHBJ1_002088"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM9431011.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACHBJ1_002089"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACHBJ1_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM9431012.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACHBJ1_002090"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACHBJ1_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM9431013.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12702..13844)
                     /locus_tag="ACHBJ1_002091"
     CDS             complement(12702..13844)
                     /locus_tag="ACHBJ1_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9431014.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4538 a         2577 c         3010 g         3819 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tgggccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaacc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag ggtctgtagg
    12601 ttaataacta tttcattgct cgaaacgttg atattaaagc gtttcgagcg tttttttatt
    12661 tgtattttaa tggttggcta cgtttttggc tacgtttgga attattcgcc cataaatttt
    12721 gcaaatcggt caccagtttt cttaacagat tgtggtgtga catgagcata gatattcatg
    12781 gtagttttaa tatctttatg acctaatcgt tcctgtactt cttttatcga cgcaccactt
    12841 tcaaacaata agctacagtg ggtatgccta aatccatgtg gagtaatttg aggctccatc
    12901 ggtgtctttt tgtaaatcca gtctaaccaa tcgtttacta cttgggggta gtacaacttg
    12961 tttgtgtttg tagtgaaaag aaactgtttt ggtgaattcg tgttatagcc atataagatc
    13021 attattttca tttgttcttg ttgccaaata cgcagttgtt taagtgtaaa atcatcaagc
    13081 tgaatcgttc ttgatgaatt tttagtttta ggtacttgta gaactacttg attaaactcg
    13141 tcttgagcga tagttttacc aattgttatt gttttattga aatagtctat atcttcccat
    13201 tgaagcgcca atgcttcgct tttacgcatt cctgtataag ctaataaacg gaaaaaagtg
    13261 tacagtttaa ctgaattatt ttctttagta ttctgtaaaa acagttgtaa ttgatctttc
    13321 gtgtaaaact tcaagggttg ttcttcttca atttttcttg gaagcaatgt tttagacata
    13381 ggattatcct tacagacttc cattgctact ccaaatttaa atatttgagc agttgttttt
    13441 ctcaagaaac catattgttt taatgttttg tgccattcat tcacctgttt ttggcaatag
    13501 ctaacactta ttttatccaa tcgtaattcc ccaaaagcag gtaatatatg gtttttgcaa
    13561 aaacgttgag caacggcaac agaagacggt tttactcttg ttctgtagtt atcaatccat
    13621 aattcataca actcttgaaa agttgtaaca ttgattttaa actcttgttt ttctttaaag
    13681 tctacaagta gtctattaag tgctgtttgc gcctcagctt ttgatttgaa tcttctacgc
    13741 tttgtacgta tatcttttcc agtcttggga tctttcccta aataagtttc aaatccccaa
    13801 tatttttcgc cattttgtaa tttataacta aaaaatgttg ccatttgatt ctctccttac
    13861 ttcattattg caggtagaga agttatttga agaatcaatt atttttaaat ttatcgtgat
    13921 tatcagttaa aaattttgcg atta
//
LOCUS       DAYYNQ010000074        13917 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000074 DAYYNQ010000000
VERSION     DAYYNQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.074"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHBJ1_002092"
     CDS             131..505
                     /locus_tag="ACHBJ1_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9431015.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHBJ1_002093"
     CDS             complement(619..1248)
                     /locus_tag="ACHBJ1_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9431016.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHBJ1_002094"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHBJ1_002094"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACHBJ1_002095"
     tRNA            1668..1741
                     /locus_tag="ACHBJ1_002095"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACHBJ1_002096"
     CDS             complement(1879..2043)
                     /locus_tag="ACHBJ1_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431017.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACHBJ1_002097"
     CDS             2338..3666
                     /locus_tag="ACHBJ1_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM9431018.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACHBJ1_002098"
     CDS             3868..4701
                     /locus_tag="ACHBJ1_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM9431019.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACHBJ1_002099"
     CDS             4717..5454
                     /locus_tag="ACHBJ1_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM9431020.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACHBJ1_002100"
     CDS             complement(5609..6577)
                     /locus_tag="ACHBJ1_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM9431021.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACHBJ1_002101"
     CDS             6809..7642
                     /locus_tag="ACHBJ1_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM9431022.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACHBJ1_002102"
     CDS             7676..8515
                     /locus_tag="ACHBJ1_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431023.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACHBJ1_002103"
     CDS             8541..9101
                     /locus_tag="ACHBJ1_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9431024.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACHBJ1_002104"
     CDS             9300..11021
                     /locus_tag="ACHBJ1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM9431025.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACHBJ1_002105"
     CDS             11186..12328
                     /locus_tag="ACHBJ1_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM9431026.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACHBJ1_002106"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACHBJ1_002106"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM9431027.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAYYNQ010000075        13899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000075 DAYYNQ010000000
VERSION     DAYYNQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.075"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHBJ1_002107"
     CDS             complement(148..2223)
                     /gene="glyS"
                     /locus_tag="ACHBJ1_002107"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM9431028.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHBJ1_002108"
     CDS             complement(2225..3142)
                     /gene="glyQ"
                     /locus_tag="ACHBJ1_002108"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM9431029.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHBJ1_002109"
     CDS             complement(3526..4338)
                     /gene="recO"
                     /locus_tag="ACHBJ1_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM9431030.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHBJ1_002110"
     CDS             complement(4481..5380)
                     /gene="era"
                     /locus_tag="ACHBJ1_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM9431031.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5395..5790)
                     /locus_tag="ACHBJ1_002111"
     CDS             complement(5395..5790)
                     /locus_tag="ACHBJ1_002111"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM9431032.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHBJ1_002112"
     CDS             complement(5774..6250)
                     /gene="ybeY"
                     /locus_tag="ACHBJ1_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM9431033.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6267..8459)
                     /locus_tag="ACHBJ1_002113"
     CDS             complement(6267..8459)
                     /locus_tag="ACHBJ1_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM9431034.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8483..9454)
                     /locus_tag="ACHBJ1_002114"
     CDS             complement(8483..9454)
                     /locus_tag="ACHBJ1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM9431035.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHBJ1_002115"
     CDS             10160..11539
                     /gene="brnQ"
                     /locus_tag="ACHBJ1_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9431036.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            complement(11693..12139)
                     /locus_tag="ACHBJ1_002116"
     CDS             complement(11693..12139)
                     /locus_tag="ACHBJ1_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM9431037.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHBJ1_002117"
     CDS             complement(12166..12342)
                     /gene="rpsU"
                     /locus_tag="ACHBJ1_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM9431038.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12504..12932)
                     /locus_tag="ACHBJ1_002118"
     CDS             complement(12504..12932)
                     /locus_tag="ACHBJ1_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9431039.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13024..13884
                     /locus_tag="ACHBJ1_002119"
     CDS             13024..13884
                     /locus_tag="ACHBJ1_002119"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM9431040.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT         3822 a         2881 c         2501 g         4695 t
ORIGIN      
        1 ttcataggtg tttttaaaag ttacacaaaa aataaaataa caaaaaaatt aacattgaat
       61 taataatagt tatttatatt tataacaaat aaaccagaga aatagcctat tttgtgcctg
      121 ttctctggtt tatttacata ttagaaatta ttttgtgatg atttgatcta aacttgctag
      181 tgacaaagcc atggcagcaa tttgacttaa ttgcgttaat cggttcttac gtactgctgg
      241 ttcttctgcc atgaccatcg tctgatcaaa ataattttcg atcagtgggc gaaggcttac
      301 aagctgatca taattttcgt tgatcgtttg gtcagcaaag cttgttttca ttgcctctac
      361 tgctttgtgc agttctttct ccgcttcgtt ttcgaacagt tcagggtcta cttctgattg
      421 tttttcaagc tccgcttttt tcgctaagtt cacgacacgt gtcaaagctt ccatcgatgg
      481 tttgaagtct tcatctgcaa gatgctgctt gaacaattgt gctgctgcaa atacttttgt
      541 cagatctttt tgttctgcat tcaaaacagc ttcgatgaca tcatgacgga tattcttcgt
      601 tgtcaataat tggcgcagac ggcctttgat aaagtcgatc acctgttgtt gtcccgctga
      661 caagccaatg ccaaaacgat ccgtatcttt actgataact tcactaatcg tttcttgcag
      721 aacagacaaa ggaaaggccc attttttcga ttcgatgatt cgaacgacac catatgcttg
      781 acggcgcaaa gcatatggat cgtttgagcc acttgggatc atcccaactg caaagaaaga
      841 aaagatacta tctaatttat cagcaatcgc taaaacagaa cctactgtag tctcaggtaa
      901 atctccttca ctggatatag gcatgtaatg ttcacggatc gctgttgcta cggctggttt
      961 ctcgccttta agcagagcat atttttcgcc catgatccct tgtagttcag ggaattctcc
     1021 taccatgttg gttactagat caaatttata gatttcagca gcgcgttgca gatccatcaa
     1081 ttcttcctca gatagtccta cttgttcgcc tatgatgcga gcgatgactt gtacccgttg
     1141 cattttctca tagatgctac cgattttttc atggaaagta acagttttta atttttcaac
     1201 gtattggtcg attgaagctt tcttgtcttc attataaaag aattccgcat cttctaaacg
     1261 agcagtcaat actttttgat tgcctcgtac cacattttcc aattttaccc gatcgccatt
     1321 acgagcagaa ataaaatgag gaagcaacat accttgatcg ttccgtactt cgaaataacg
     1381 ttgatgttct ttcatagaag tgactagtac ttcttcagga acagatagat atttttcttc
     1441 gaagtcgcca gaaaagacag ttggatattc gactaaattg ttgacttctt ctagtaaatc
     1501 ttcatctaga tcaatgatcc agtgattttt ttctgctaat tcgtttgctt gtgtgatgat
     1561 catttctttt cttttttgcg gtgaagcaat aacaaattgt tcattcaatt tttcttcgta
     1621 ttgttttgca tgcgtaaatg tcacatcttc gcctaagaaa cgatgacctc ttgaagctct
     1681 gtctgttttg acatccaaga tctcaaacgg gattatttcg tcatctaaca atgccacgat
     1741 ccagtgaata gggcgaatat attcaaaatc gtattctgcc cagtgcatcg ttactgggaa
     1801 agtgaggctt ttaatgacat ctgctaaacc tgtcaatact tcttttgctt ctttgccgtg
     1861 agtgtatttc gttacataga catattccac gccttttaat tctctaaaag tgatagcatc
     1921 tgtcgtcaga ccttgtccgc ggacaaatcc ttcagctgct ttcgtccagt ttccatctgc
     1981 gtccaaagca atttttttgg ctggtccttt gacttcttct tcgctgtctg cttgacgttc
     2041 tggaatgttt gttactttga ttgctaagcg gcgcggtgta gaaaacgttt cgatcgtatc
     2101 ataagtcaat tggttttcat ctaaaaattt tgctgttttt tcttctaatt gtttcatgct
     2161 tggtgtgact acatgagcag gtatttcttc cagcccgatt tcaagtaata agtcttttgc
     2221 catcttattc agcctccttc acgttttctg gatttgctgt gtttttattc aataaaggga
     2281 atcctaattt ttcgcgttct gccacaaaga ttttggctac agcacgagcc atgttgcgga
     2341 tacgggaaag ataacctgca cgctctgtta cggatactgc accgcgagca tctaataaat
     2401 tgaatgtatg gctacatttc aaaatataat catacgctgg atgaacaaga ttttcatcga
     2461 tacagcgttt agcttctttt tcaaaacgat caaaattgct tagtagcagg tcttggtcgc
     2521 ttacttcaaa agaatattta gaatgttcat attctggttg tttgaagatc tcaccatatt
     2581 tcacgccatc agcccattcc agatcataaa cactttcaac ttcttggata taagaagcaa
     2641 gacgttctag tccataagtg atctcagaag taactggttt acaaggtagt cctccgactt
     2701 gctggaagta tgtgaattgt gtgatctcca tcccatcaag ccatacttcc cagcccacac
     2761 cggcacaacc catcgatggg ttctcccagt tgtcttcaac aaagcgaata tcgtgttcta
     2821 atggatcaat tcctaaaaga cgcaagcttt ctaaataaag ctcttggata ttttcaggtg
     2881 aaggtttcat tacgacttga aattgatgat gctgatacaa acggtttggg ttttctccat
     2941 aacgcccgtc agctggtcgg cgggatggct ctacataagc agcattccat ggttccggtc
     3001 cgattgcccg taagaatgta tatgggctca tcgtacctgc tcctttttct gtatcataag
     3061 cttgcatcag catgcagccg tttgatgacc aaaatttttg taatgttaaa atcatatctt
     3121 ggacggtcaa ttttttactc atctgttttg cccctttcaa tttttaagcg ttgctgtcaa
     3181 taagaataca ggaaagaaaa cgacaaaaag tccctatgtc tgattacaga catagggacg
     3241 aataatttcg cggttccacc ctacttccga ataaagattc ggcagcttca tttatcgtac
     3301 tcgcaagtgc ctcttcaaaa atcgtttggg tccggcttcc actctcccga actcgctaaa
     3361 ccacgtgttt tttactgatc ttggtcaatg tacgtattca ttttgcagtt ttctggtttg
     3421 tataatagat cctctcagtg aacgatctat ttaatctacg gttaataata gacggaagtc
     3481 cctgtatttg tcaaatagtt ttgatttttt tgttgtatct ttcatttatt cttctttttc
     3541 aggtatctct ttttcaggca tatttttctc tggcgtcttt aatacgtgtg cccaagactt
     3601 catttgatca atgaactttt tgctttttag atgaataccg acatattcat catacaactg
     3661 gtcaatgagg tcacggattg cctgcttcgt ttcttctttc aagtggattc cactgatctt
     3721 atcataagaa atagccgaaa acattcgaac gaaataaaca gcgcgaggat ctgcatgata
     3781 acggtgttcg tccattggcc agtgtttctc gcatatcaca ccgccatatt tggaagaata
     3841 gtcaaatttt ccttttgttt ccccgcatac gacacagtgc gtccaaattg gcgcgattcc
     3901 gaagcgttgc agtatttgca cttcaaagat attcgtgatg atttctgcat catctccttg
     3961 gtccatccgc tgcagagctt gttgcgtaaa ttggaataaa tgcgggtcat attgatgatc
     4021 ttcgattgct gcatctacta gatttagcaa atacgtacca taaccgctca aaaagatatc
     4081 ttcttggatg ttccgaaacg catggacgtc tttgacactg cttaaaaacg acaaaccttc
     4141 acttcgaaaa ttaccgatat agactgcttc ggtaaagggg agcaaagccg gagctaatgg
     4201 ctgattttgc cgatggattc ctttgatata aaacatcatt ttgccagaag attccgtaaa
     4261 gattttgact aatttatctt tttctttgta attacggcta gataaaatca agcctttcga
     4321 ttctgcaagc tggctcatat tctccctcct aaaaaataaa tctggaaaag cagattctgc
     4381 cgatccagat tatttatgtt tcgatcttcc gctgtttttc tcaacgtacg gatttttatc
     4441 cagtttattt ctatacctaa cctataagaa gtccagaata tcagtactcg tcttttcggt
     4501 agccgaagtc ttgcagatag acttttttgt ctcgccagtc tttttggact ttgacccaaa
     4561 gttctagaaa tactttgtct cctaaaagat tttcgatatc ctttcttgct ttcgttccta
     4621 cttgcttcag catttttccg cctttgccga taatgattcc tttttggcta tctctttcga
     4681 cgatgatcgt ggcctggata tggattttat cattctcgtt tcgcttcatc gaatcaacaa
     4741 cgactgcgac agaatgaggc acttcgtctc gtgttaaaaa caagactttc tcacggatca
     4801 attcagaaac gatgaagtat tcaggatgat cagtcacttg atcatctgga aaatattgtg
     4861 ggccctctgg catttcactg actaatacat ccattaagcg ttcgacattg tttccttctg
     4921 tagcagagat cggaatcact tgtgcaaaag gcatctgcgt cgaataatct tcgatgattc
     4981 ccagcaactc gtctggatgg atcttatcga ttttattgat aatcagataa acaggagaat
     5041 gcacgttttt tagacgttca ataataaaat cgtctccccg gccacgtttt tgatcagcac
     5101 tgatcataaa taagactgca tccacttctt tcaacgcact gtaagctgct tctaccataa
     5161 aatctcctag acgatgcttt ggtttatgga ttcctggtgt atcgataaaa atcagctgag
     5221 cttctggtgt agtatatact ccttggattt tatttctagt tgtctgtgcc ttgtcactca
     5281 taatggcaat tttttgaccc acgattcggt tcaataatgt ggattttcct acattcgggc
     5341 ggccgacgat tgcgacaaac cctgatttat gttctggcat atacgttctc ctttttaatt
     5401 aaacaacaaa tgatatattt tagggacaaa taaaatcaac ccaatgatga cagcaaaaca
     5461 agcggtcact aaaactgctc cagctgccat atctttgatt tttttcccaa tcggatgaaa
     5521 atgaaaatcc gtaaacatgt ccactacatt ttcaaaaaca gtattgatga tctctactat
     5581 ccaaacgaga aaaacagcta aaagcagcca taaccactct atccgatcaa gctggaaaat
     5641 cacgcccata aataaagcaa ggataccgaa cgcaacatgt ttcctcatat tgcgttcttc
     5701 ctcaaaaacg gttttgactc cttggacagc aaattccaca gacatcagga aatgtttgtt
     5761 tttctctacg tttttatctt ttgagtccat aggcatccaa gatctctttc tgtaaaccaa
     5821 acatttcttt ctcatcttct ggtgtcatat ggtcataccc gttgatatgc aagaatccat
     5881 gtaccgctaa aaagccaagt tcccgatcat atgaatggcc gtaagcttct gcttgttcag
     5941 cagcacgttc aatagagatc atgatgtctc ctagtacacg aggtaatgga ttctctccat
     6001 cttcaaaaat cactgggatt tcgtcttcgc cttcttcttc cagagcaaaa ctgatgacgt
     6061 ctgtcggtgc atcttttcca cggtaatcac gattgattac ttggattgcc gcattgtcca
     6121 tgaaagtaac agacatttct gtttcctctg gcagctttaa ataagatgct gcaaattgca
     6181 gcaagtcatc gatttctttc attttctctt ctgagacttg gtttgtttca tccataaagg
     6241 taatatccat tatacatcgc ttctttctat tcttcttgtt cttcctttat cttctcaact
     6301 tctgttttca ttttaggata tttgatccga gaatggaagg tgctacttaa accgctgatt
     6361 aatgattttt ccacttttcg gatttctttc aaggtcagtc cactatcgtc taattgacca
     6421 tcggaaatac gctcttcgat aagattatgg acaaaacttt cgattttttc gctcgtcgga
     6481 tgatccattg cccgtactgc agcctcacaa ctgtcagcaa tattgacgat ccctgcttca
     6541 cgactttgcg gttttggtcc aggataacgg aattgctctt ctgttacttc cggattgcgt
     6601 tctttcgctt tgatgtagaa ataacgcatt agcgtggttc catgatgctg tttgcaaata
     6661 tcgatcacca tttgcggcat attataatct tccaatattt tggctccatc tgtcacatgt
     6721 ccaaagataa tatgtttact gtcttctggc aataagaaat tgtgcgggtt ttccgctcct
     6781 gaaggcaagt tttcaacgaa aaagctagca tgtttgatct tgccgatatc atgataatag
     6841 caagctactc gagttagcaa agaacgtccg ccgatttctg ctacagcgtt agcacttaga
     6901 ttagctacca tcatactatg atgataagta cctggcgctt cttccagcaa ctgtttcaat
     6961 aaaggatgat ttggattgct gagttcattt aatacaatta cactgtcatc attgaccatc
     7021 aattcaatat atgggtgtag accgatcgtc agcaagaatg agaagagtgc accagtgaac
     7081 ccgcagacga gcattaacca agtcgttgca tcaccaaagc tcattccttg atagacgatc
     7141 aaaatgacat ccatcatcac aggaaaaata atgacccaca tggcagcaga taatccttgt
     7201 tctgagatcc gtttgcgtct gacaatcgtt gccatgagcc cagagaaaac ataagacatc
     7261 aagataatcg ttagaaagtt cgtgccaatc gaatcataaa aaatgaaaag tgcggatact
     7321 gcttggaaaa taccagccat gatccctgct cttcgattca agaaaaagct taaaacaagt
     7381 ggtgcaaaag cagcgggata aaacagcggg atataagtag cttgttccgt ttggaataat
     7441 tggaaaaatt tcatcagcac tatgctgact gacatagctg tcacatagaa aagcacgtat
     7501 tttgttcttt ctcctgcatt ttggaattga agtgaattat aaatcagtac gccgatttga
     7561 agcaaaacag ctaaaaccat ggcaattagc ggaaaaatcg aagttgtttg attagtcagt
     7621 cctagtaatt ccaatttttt catagctgaa gcatcgattt gattaccttc acgaacaatg
     7681 atttctcctt ggaagatcat cactggttgt acagcttctc gtgcggattg tttcaattct
     7741 tctgtctttt tctcattcaa tacgtcattc actacgatac cttgatccac taagtagcga
     7801 atcatttgtt gttgtgctgg tgtgacattc aaatattgga tttgctcttc tgcttcttgt
     7861 ttaaaagctt ccaattcact ttcacgaact tgtttgctca tctgttcgtc aatgagcttc
     7921 agactctcat ccctcacagt atgaatctca gtttcagtca tttcaaatat cgtcgaataa
     7981 aaattgttcg gtagtttttg ataaaacaca acattttctg cattcacatt ttcaaaattt
     8041 ttcttcagcg cggcgatccg ctcttctgta gtaggctttg ggatattttc atcttccttt
     8101 gccttatcaa tcttttcgtc ataagatttg ttgatggcat cattcgtctt atcgatcaat
     8161 tcaaatagtt gcttgatccg attgttctgg tcatctgcaa gatctttttg gtaagtgtat
     8221 tctggagtga ctgcttctgc tgccagtttt cttttttgtt cggtttcttc cgtgttctcg
     8281 atcgttttgt tcgcacggat actttcttct gctacttggc cttctctgaa atcattggcc
     8341 ttttgtttga cactactaaa tgtaacgccg cagataatga taaaaaacaa gaacagcagg
     8401 aaaggcaatg ctgcctttcc aagtttttct tgtagtttct gcagtgtttt attcaacatg
     8461 tgcttatcct cctaagcaaa gcttattctt gatggagatc tgccttttca taagctttga
     8521 tgatttcggc aactacaggg tgccgaacaa cgtcacttgc ttcaaaatta acaaaatcga
     8581 ttttcttgat ttgctttaat gttcgttctg catgaacaag accacttgtc gtccctcgag
     8641 gcaagtctat ctgacttgta tccccattga caatcatttt tgattgataa ccgagacggg
     8701 tcaagaacat tttcatctga gcgactgtcg tgttttgtgc ttcgtccaag ataacgaatg
     8761 catcatccaa tgttcgcccc cgcatgtaag ctaaaggtgc gatttcgatt acgcctcgtt
     8821 ccatcaatcg gttcgtatga tccaatccaa aaatttggta taaagcatca taaacaggcc
     8881 gcagataagg atccactttt tcttttagat ccccgggaag aaagcctaga ttttcacctg
     8941 cttccacagc aggccgcgtc aaaatgattt tttgcacttc accttttttc aaggcagcga
     9001 ttgctaatac tactgcaaga aaagtttttc ctgtaccggc tggtcctacg ccaaatacga
     9061 tatcatgttt ggctaccgat tcgacatatt tcttttggcc gctgtttttt acccgtatag
     9121 gttttccatt acggtctttg acgatctctt cctcatacat ctcaacaaaa taatctaatg
     9181 tacctttttg ccccattttc aatgccgtca caacatcggg cgtacttacg tggattcctc
     9241 gtttgatcaa ttcctgtaat gtccgaatga ctgaggcagc caaggaaatc tgggattgct
     9301 caccaatgat ctgaatgatt tctccacgtg tatggatggt tgtttccgtt gtttcctcaa
     9361 tcagttttat atgcttgtcg tgtgcaccta aaagcatgct gacatcatca gcagccgtca
     9421 atctaatctc taaagaacta gactcttccg tcaaaaatag acatcccctt tttttcgatt
     9481 cattctttcc tattttacca tactcagaag aaactatata gcaaataaga aaaggttcta
     9541 aaagagttgc ttaaccataa gtaccaagaa acgttaaaaa actagccgct tccattctct
     9601 aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa
     9661 gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa
     9721 caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa
     9781 taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga
     9841 gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt
     9901 cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac tttatcgaaa
     9961 agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta
    10021 gagccctttt cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg
    10081 aatctgatat tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata
    10141 attattggag gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat
    10201 ggtcagttca cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtaca
    10261 cttaggtcaa atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg
    10321 cgctcttttc ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta
    10381 tgacttagct aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt
    10441 aacgatcgga ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc
    10501 taaacccttt ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt
    10561 ctttttactt gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa
    10621 gtatttaaac tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca
    10681 acccatgggg aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa
    10741 tggcgtttta gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac
    10801 ttttgtccat gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc
    10861 aaaagctggt agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct
    10921 cggtgcgatg agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca
    10981 gatcactaat ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt
    11041 aggtgtcttc actactgcta tgggactcgt tgtttctttt gcacaagatt tccataaact
    11101 gttccctaaa gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt
    11161 tgcgaatgcc ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta
    11221 tccattgtca cttgcgttaa tcttactttc cttaacagca aaattcttcc aaaagagtcc
    11281 atttgtctat caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc
    11341 aaattcgcct gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca
    11401 tcattatgtt ccttttgctg cattaggtct tggctgggtc gtcccaacgt tgcttggtta
    11461 cgctggcagc ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag
    11521 tgagttacca gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
    11581 aaaaaacagc tagaagaaag aataagattc tttcttctag ctgtttttta gttctttttt
    11641 tacgaacaca aaaaactgcg gataatcact gtccgcagtt taaatgataa aattaagatt
    11701 gtaatagttc tttcacaatc gcattcactt ggtttccatc tgcttttcct ttgactttag
    11761 gcataattgc acccataact ttgccaaatt ctttcattga tgaagcacca gtttgagtga
    11821 ttgcttcttg aacaagttgt cgaatttctt cgtcggaaag ttgttccggc atatattttt
    11881 cgactattac aatttcactt tttacttttt cagctaaatc gtcacgtcct gcttcttcaa
    11941 actcatgcaa agaatcacgt cgctgtttca tttcacgaga caacaccgtt agttcttctt
    12001 cttctgtcaa gtcgtgacct tctttaatct gctcgttttg tatagatgac ttgatcattc
    12061 ggatgacttg taaagattcc ttatctttcg ctttcatcgc tgctttcatg tcatcgttta
    12121 atctacttag tagtgacaca ctaccaactc caattcatca cagaactaga attttttgcg
    12181 ttttctagct gcttcagatt ttttcttgcg tcttacactt ggtttttcgt agaattcacg
    12241 tttacgagat tcttgtaaag tacccgcttt tgacacggaa cgtttgaagc gacgaagagc
    12301 atcgtcaagt gattcgtttt tgcgaacgac tgtttttgac atgttaattc cctccctccg
    12361 agcttaaaaa gtaaccgtaa ttgttattga aatatatctc aaaaaacaag actgtcctgt
    12421 aaatattata tctaatgctg ttttgaccgt caaggaaatc ttgctttttt ttaaaattat
    12481 caaatgttat tttatttaaa tccttattga caattttcac atcttccgaa aatctcgaat
    12541 ctatgccctt cgatcgtgca tccacctagc tgttcttcga aataattcat cggacacata
    12601 tggatttctt tcgttgttcc gcacactgta cagataaaat gatggtgatg gccgattttc
    12661 ccctgacagc aatgaaaacg aaacttcatt tctccattta aatcggtttc ttccagtaaa
    12721 tcgatctctg caaaatcatg gagattccgg taaatcgtat cataacttac ccctttgaat
    12781 tcctgattca tatagtcaaa aacttcttta gcagatacgt agcggttacg cttcactaaa
    12841 taagttaaca atgcttctct ttttttagta tatttaaaac cgttattttt caatgtttct
    12901 aatgattgtt cgatcaatgt ctgttttttc atgacttatt cctccaaatc aaaatgatta
    12961 cgaagtacgc attctagagt atggtataat aaacataaaa aaaatgcaag tgaggtttta
    13021 tccatgtctt ccgattctac agaaatcgtt actttttttg tcatctccga ctctgctgga
    13081 gaaactgcga caaaactagc gcaagctact atggctcagt atcctaccgt tgagttcaac
    13141 ctattccgca gaacgttcgt gacagatgaa aaaacattaa ggcaggcatt gcaagatgca
    13201 ttagaagaaa aagcacttgt cctgcataca ttaatcaatc aagaactgat cgagataaca
    13261 cgttcttttt gtgaagaaca tcaattattt agttttgatg tgatgacgcc accgatatca
    13321 gaaattgaac gtttaactgg tatcaagcca attcggcaac ctggtgccct ccatttattg
    13381 aatgagaact atttcaaacg gatcaaagca atggaatttg ctgtaaaata cgatgatggg
    13441 aaagatcctc gtggtttttt agaagcagat gtcgttttgc taggcgtatc ccgaacttcc
    13501 aaaacgcctt tgagcttgtt tttagctaac aaaaatttaa aagttgccaa tttacctttg
    13561 atcccacaag cacatattcc caaacagctt tgggagatcg atcctaaaaa aatcgtggga
    13621 ctaacgaata acccagatat tttaaataat atccgtaaag aacggatgcg ctcttacgga
    13681 ttaaaccctg atactgctta ttccgatatc gaaaaaatta gagctgaact tgattttgcg
    13741 aatgatcttt atgaaaaact aggatgtgta gtgatcaatg tggcatcttt atctattgaa
    13801 gaaacggctt cgcttatttt gaatgcttta gacctagaag atcatagtta ttatggaact
    13861 gaaacaacag aaaaaagcga ttaatcattt ttgtctttt
//
LOCUS       DAYYNQ010000076        13766 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000076 DAYYNQ010000000
VERSION     DAYYNQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.076"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..306
                     /locus_tag="ACHBJ1_002120"
     CDS             112..306
                     /locus_tag="ACHBJ1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431041.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="ACHBJ1_002121"
     CDS             575..874
                     /locus_tag="ACHBJ1_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431042.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="ACHBJ1_002122"
     CDS             complement(971..1405)
                     /locus_tag="ACHBJ1_002122"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9431043.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="ACHBJ1_002123"
     CDS             complement(1637..2347)
                     /locus_tag="ACHBJ1_002123"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM9431044.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="ACHBJ1_002124"
     CDS             complement(2337..3197)
                     /locus_tag="ACHBJ1_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM9431045.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="ACHBJ1_002125"
     CDS             complement(3202..3840)
                     /locus_tag="ACHBJ1_002125"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM9431046.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="ACHBJ1_002126"
     CDS             complement(3855..4154)
                     /locus_tag="ACHBJ1_002126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9431047.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="ACHBJ1_002127"
     CDS             complement(4197..5681)
                     /locus_tag="ACHBJ1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM9431048.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="ACHBJ1_002128"
     CDS             complement(5701..6162)
                     /locus_tag="ACHBJ1_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9431049.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHBJ1_002129"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="ACHBJ1_002129"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM9431050.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="ACHBJ1_002130"
     CDS             7465..8235
                     /locus_tag="ACHBJ1_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM9431051.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="ACHBJ1_002131"
     CDS             complement(8508..9335)
                     /locus_tag="ACHBJ1_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431052.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="ACHBJ1_002132"
     CDS             9903..11138
                     /locus_tag="ACHBJ1_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9431053.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="ACHBJ1_002133"
     CDS             complement(12137..12397)
                     /locus_tag="ACHBJ1_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM9431054.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="ACHBJ1_002134"
     CDS             complement(12424..13725)
                     /locus_tag="ACHBJ1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9431055.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4422 a         2823 c         2169 g         4352 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgc
//
LOCUS       DAYYNQ010000077        13531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000077 DAYYNQ010000000
VERSION     DAYYNQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.077"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACHBJ1_002135"
     CDS             99..746
                     /locus_tag="ACHBJ1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM9431056.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACHBJ1_002136"
     CDS             1139..3784
                     /locus_tag="ACHBJ1_002136"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM9431057.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACHBJ1_002137"
     CDS             4094..5407
                     /locus_tag="ACHBJ1_002137"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM9431058.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACHBJ1_002138"
     CDS             5397..6050
                     /locus_tag="ACHBJ1_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9431059.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACHBJ1_002139"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACHBJ1_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM9431060.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACHBJ1_002140"
     CDS             6947..7147
                     /locus_tag="ACHBJ1_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9431061.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACHBJ1_002141"
     CDS             complement(7401..9080)
                     /locus_tag="ACHBJ1_002141"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM9431062.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACHBJ1_002142"
     CDS             complement(9082..9294)
                     /locus_tag="ACHBJ1_002142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM9431063.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBJ1_002143"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACHBJ1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM9431064.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBJ1_002144"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACHBJ1_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM9431065.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACHBJ1_002145"
     CDS             13269..13466
                     /locus_tag="ACHBJ1_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431066.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
BASE COUNT         4292 a         2350 c         2911 g         3978 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag ttgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct g
//
LOCUS       DAYYNQ010000078        13367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000078 DAYYNQ010000000
VERSION     DAYYNQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.078"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(58..2139)
                     /locus_tag="ACHBJ1_002146"
     CDS             complement(58..2139)
                     /locus_tag="ACHBJ1_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM9431067.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2281..2403)
                     /locus_tag="ACHBJ1_002147"
     CDS             complement(2281..2403)
                     /locus_tag="ACHBJ1_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431068.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            complement(2663..3022)
                     /locus_tag="ACHBJ1_002148"
     CDS             complement(2663..3022)
                     /locus_tag="ACHBJ1_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM9431069.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3052..3393)
                     /locus_tag="ACHBJ1_002149"
     CDS             complement(3052..3393)
                     /locus_tag="ACHBJ1_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431070.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(3390..4064)
                     /locus_tag="ACHBJ1_002150"
     CDS             complement(3390..4064)
                     /locus_tag="ACHBJ1_002150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM9431071.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(4998..6296)
                     /gene="asnS"
                     /locus_tag="ACHBJ1_002151"
     CDS             complement(4998..6296)
                     /gene="asnS"
                     /locus_tag="ACHBJ1_002151"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM9431072.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6336..7526)
                     /locus_tag="ACHBJ1_002152"
     CDS             complement(6336..7526)
                     /locus_tag="ACHBJ1_002152"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9431073.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7547..8074)
                     /locus_tag="ACHBJ1_002153"
     CDS             complement(7547..8074)
                     /locus_tag="ACHBJ1_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM9431074.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8121..10910)
                     /locus_tag="ACHBJ1_002154"
     CDS             complement(8121..10910)
                     /locus_tag="ACHBJ1_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM9431075.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11057..11257)
                     /locus_tag="ACHBJ1_002155"
     CDS             complement(11057..11257)
                     /locus_tag="ACHBJ1_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9431076.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11848..12954)
                     /locus_tag="ACHBJ1_002156"
     CDS             complement(11848..12954)
                     /locus_tag="ACHBJ1_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431077.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
BASE COUNT         3698 a         2897 c         2309 g         4463 t
ORIGIN      
        1 ttttcattaa gaaaaggata taaaaaaaag aggacgttca ttcgtcctct tcattgatca
       61 acgtttattt tttacatgca agcttcgcat ggcattcaaa acagctaata ctgtcacccc
      121 aacatctgca aatactgcat ctcccattga tgcaaaacca aaagcaccta aggaaagtac
      181 caaaattttt atcccaatag caaagacaat gttttgtttg acgattttca atgttttctt
      241 tgctaaatgg attgcttctg caatcttcga tggttgatca ttcatgatga ctacatctgc
      301 tgcttcaatt gcagcatcgc ttcccaatcc acccattgcg attcctacat ctgctcttgc
      361 tagcaccggt gcatcgttga ttccatctcc gacaaatgcg gtctttttat tgttactttg
      421 taagatttcc tctaaccgct gcactttatc ttctggaagc aattcgctat atatcttgtc
      481 cacgcctact tgcttcccga tatgatcagc aatttttttc gagtcgcccg tcaacatgac
      541 tgtgtttttc acaccagcct gttttaattg aaccaatgcg tctacggcat cttctttcaa
      601 ggtatctgca ataacaagat aaccactgta actttgatcg attgctagat aaagcaatgt
      661 accgatttct tgacttgaag gaaaagagat ttcaaatctt tccatcaatt tgtgattacc
      721 tactaatact tgtttgccat caacttcagc taagatccct tcacctgcaa attcctggat
      781 attagctgtt ggtaaaagcg gacctgcgta gccttctaaa acagattgag caatcggatg
      841 tgtagaaaac tgttctgcac tcgcaatata ttgcatgaat atctttggat cgatttttgt
      901 atcgatcgtc tggatcgaaa agtcgccttt cgttaatgtg ccggttttat cgaataccac
      961 tgtctcggtt tgcgcaagca actccaagta atttgaacct tttactaata caccgatttt
     1021 acttgctcct ccgataccgc caaagaaact aagtggcacg gaaatcacta atgcacatgg
     1081 acatgagatc actaaaaatg tcaatgctcg atagatccat tcagaaaacg ccgctccaga
     1141 aacaacaagt ggaggtaaaa cagctaataa aacggccaat cctacaacga ctggtgtgta
     1201 atagcgcgcg aatttagtaa tgaaattttc agctggtgct tttttactgc tggcattttc
     1261 tactaattct aataatttgc tgatagtaga attttcataa gatttttcta cttgaactaa
     1321 aaatggttta tttttattga taaatccgct taatactgtt tcaccaacat atacagattt
     1381 agggactgat tctcctgtaa gtgctgacgt atcgactaat gattctcctt cgatgatcgt
     1441 tccatctaac ggtacacgtt caccaggctt cacaagtaca aactgaccga tcagcacgtc
     1501 ttcagggttc acggtcatca atccttccgc cgtctgcacg tgagctgttt ctggacggat
     1561 cgccatcaat tcgcgaatcg attttcttga atgactgact gccaatcctt ggaaatactc
     1621 gccgatttga taaaatagca tcactgctac ggcttctgga tattcaccga tcaacatagc
     1681 gccaatcgta gcaacagaca taagaaaatt ctcatcaaag atttttcctt tgacgatatt
     1741 catcactgcc ttcttcacta catcatatcc tatcagcaga taaatcaata aataccctat
     1801 caagcgaatt agttcattaa aatcaaataa tagcaataaa gcgatacctg caagggaaaa
     1861 taaaatccgg aacagcgtat ttctctcttt ttcatttcca tgttcagata ctttttcctt
     1921 atctatttct gttactttga catctggttc taattccttc actacttttt ttgtttcttg
     1981 ttcaatcgta gccaaatgat cttcctccgc gtcaatcgtt agttttccag aagtgaagtt
     2041 taccgttgct tccttgactc cattgatttt ctgaactcct ttttcgattt tcattgcaca
     2101 gttcgcacaa tctaaacctt ctaatctgta acttttcatt ttaatgacct cctcgcctca
     2161 acatatgaat atctcttcat aactaattat aaatgaatgg ttattcatat gtcaaggtat
     2221 ccgaaagaaa aaatgaaaaa catctgcatc ggaaaatttc ttccatacag atgttttggc
     2281 ttattctttc tttttctctt cgatcattgc cactgctctt atccaacccg cacttgcctt
     2341 cttgatcttg acaggatagc aactaagttt gaagcctgtt gctggcaatt tgtctaaatt
     2401 cattaatttt tcgataggac aatatctttt ttcaatccct gcaaaatgtc cttcccagat
     2461 atacgaagct ccactacgct ttcaaaaata tagaaagagt tctttaatga aaattatttc
     2521 aactttccat aaacagtaac cccatataaa taaaagacgt aaaagtcaca tggaagaact
     2581 tatacaggaa aacgcataga gtcaggtgaa atacatgtat cccacctgac tctatgcttt
     2641 atctaacctt tattattctc ttttatacaa tttctgcacc gaatggcatc aacgccaatc
     2701 gagcaagttt gaaagattgc ttggcaaaag gtatccctat gatcgtgata catagaagga
     2761 tgccgctgac gacatgtgcg agagcaattg gtaggcctga aacgatcagc cagatgatat
     2821 taacgatcaa tgacatgcca gactggtgat aaattacttc tttgccaaaa ggcgcaaaag
     2881 aaaggaaggc gatcttaaaa cattgcatac cgattgggat acctatgatc gtgatacacc
     2941 aaagtatccc cgctgctaac cagctaagac caccgattag tccgccgaat ataaaccaga
     3001 tgacatttcc taaacaactc attgtatttc tccttctatt actgtttttg attaaaactg
     3061 ggcaaaaatt gattttattc ttcttttttt agcttccagc atcaaatatt ctgcaaattt
     3121 tttttggatt tcttccgtat tttcttttgt tactaatgtg tgttggatta gttttcctga
     3181 tggtcgctga cccacccgtt cttctgactg ttgcacgtcc gcttgcgcaa aaccgataat
     3241 ccgtttttct tttgttacag caacgagcaa tagtttattc ttatcattta ggtcagctgc
     3301 cgtttttgct tttacgaatg attctggatg atcagtaatc agctgattga tttgaggtag
     3361 atcttctttt actgcccacc gtatttccat catttctcac ttttctttcg ctcattttta
     3421 tctccactaa aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc aatgatctca
     3481 tgtccgccaa tcgttgggtg gaccccatct tctcttgtat atttcgaaaa accataagca
     3541 atccctaaag cattgaatat gccatctaaa ggaaccagca atgcgccata ttccttagca
     3601 agtttacgaa caatttggat tttagggtct aagtctttac gccagccatg acggtcgtcg
     3661 gttataggaa gtacaaacgg ctccatcaag atgatgttgc agattcccgc tttttgaagt
     3721 tcatttagta attttcgata aacggattca aattgctgag cagatttctc agttccgaat
     3781 atagcctgat cgctagtatt atgccaggta tcattgattc cgatcaaaat cgatacatag
     3841 tcaggttgta aagctaaaca atcttctcgc cagcgtactt gcagatctgc aattttgtct
     3901 cctcctatcc cacggttcat gcattcaaat tccatcgttg gatattggga gattagtttt
     3961 ccggcaatca aaagtggata acctttacct agatcagttg cttctgctcg gtctcttcct
     4021 gcgtctgtaa tgctgtcacc gataaataat atacgttttt tcatttgtca aaaactcctt
     4081 tttgaataaa acgatgattt tcttccttat attatcccat aagccctatc aagacgaaag
     4141 agaaaatata tgtataaaaa atgacaccag cctagtccct tcaaacaatt gaatgaacgc
     4201 tcatcaagtg tctagttttt tatgctattt ttttcttaac gtgctggatt ccccttgatt
     4261 ccttcattgt ttggtccaga gagatcgatc acacctaagc ttccgtcaat cagaccactt
     4321 gatgacgcca tcagaacata tatttatagg ctcatctaag acttccagta aatgttcctt
     4381 tactaatatc tcttctagat tgcttgggat agattggcca taactttatc tggtccgatg
     4441 atttcaaggg tgaattgaac ttacatatgg atcattgcat gctgaaaata cggacctact
     4501 ccatcttcga atgttactat tagtttgttt tcaggtgcta gatgctgctt tatttcaaca
     4561 gcagaagcat catcaaatca gatttccatt tgaataacct tctttgtata tgtttgattg
     4621 attgcgattt cgttatagaa ccttgtgaat caaatagcaa tttcttttgc tttcttctct
     4681 tatctgctgg aaacttcctt tatcccgtat ttaaaaaaaa agaactgcga gagttcgcag
     4741 ttcttttttt taaataaacg ttgctctccg gccaactcct tggtcacaag tcacaatagt
     4801 catcaaacat gaaaaaatgt ttttttgaaa ttactccttg tgactcggag ctgaacggca
     4861 gacgcacaac ctcttgatat aaacggtgtt caatcagctg accctcgata ttagctcaaa
     4921 aaacagtaaa atatgagaag ctatttttcc gttttgtttc tctaatactc aggtcaaggc
     4981 actgatttca caacctctta tggataaatt cggttcaaca aacgtgggaa tggaatagct
     5041 tcacgcacat ggtcgatacc agcaatccaa gtgactgttc gttcaaggcc tagaccaaaa
     5101 ccagagtgcg gaacagaacc gtatttacgt agatcaagat accatgaata ttctttttca
     5161 tccaaaccgt gattacggat ttgttctagc aagaaatcat agtctgttgc ccgttcgctt
     5221 ccaccaataa tttcaccgta accttctggt gcgatcatat ctgcacagat caccacgtct
     5281 tctcttgttg gatgtggttt catgtagaat ggcttgatgg cttttggata gtttaggata
     5341 aagacaggct gttcgaatga attcgcaatg aacgtttcat gaggtgaacc aaagtcttcg
     5401 ccccattcga tgtcatcaaa tccatttttc ttcagtaatt cgatcgcatc atcataagta
     5461 atacgaggga atggcaattt tgtataagtt tttaagactt ctttgtctcg tcctagtaca
     5521 tctaatgcgt attcgcaatg atctaaaaca ttttgtacta agaatgctac atattgttcc
     5581 tgtacttcta aactttcttc ttgatgagtg aaagccattt ctggttcgat catccagaat
     5641 tcgatcaaat gacgtcgtgt ttttgatttt tcagcacgga aagtcggacc gaatgaaaag
     5701 acttttccga aagccatagc agcagcttcc atgtataatt ggccactttg agataaataa
     5761 gctttttgat caaagtaatt tgtttcgaat agatccgtag tgccttctgg tgcagaacca
     5821 gtcaagatcg gcggatcgat tttgacaaaa tgatgttgat taaagaattc atacgttgca
     5881 cgaatgattt catttctgat ctgcataatc gcatgttgtc ttgaagaacg cagccataaa
     5941 tgacgatgat ccatcaagaa gtctgttccg tgttcttttg gtgtgatcgg ataatcgtgg
     6001 ctctcgctta ccacttccac atttgtaatt ccgatttcat aaccaaattt tgaacgactg
     6061 tcttcacgga tttctccagt tactaacacg gatgtttctt ggtgtagatt tttagctgtt
     6121 tggaaaactt cttccggcac ttcgcttttt acaactacac cttggaaaaa ggctgttccg
     6181 tctcttaatt gcaaaaatgc gatttttccg cttgatcgct tatttgcgac ccaagcgccg
     6241 attgttactg tttctcccac atggtttttc gaatcgatga tttgaatttg ttccacgtat
     6301 gactctcctt ttatattcct tgaatttctt caggattaat tttgactttt actctcaata
     6361 aattgcttga ttctgcgtac cgcttcttct aacgtagcgc gatcagtggc ataactcatg
     6421 cggacatttt ctggtgcccc aaagccttcg ccagtcacaa gcgctacccc tgtttcttct
     6481 aaaagatctt ctacccattg tgtgacattt tcatatttac acatgcgtag cgtttctttt
     6541 acattaggaa aaagataaaa agctccttgt ggcttttcaa gagaaacacc cggcagttct
     6601 gccacaagcg gatataacgt attcaggcgt tcttcaaagg cgttccgcat ttcttccact
     6661 gtatcttgct cgcctttcaa tgcttcaaca gctgcatatt gactgactgc tgtcggatta
     6721 ctggttgact gggaagcaac agcaatcatt ccattgatga tctcagaatt ccccaccgca
     6781 taaccaatgc gccagcctgt catcgcatat gttttagaca cgccattgat gatgatggtc
     6841 tgatttttga ttgcttctga aatcgttgca attggagtaa actcattgcc attataaact
     6901 aaacggccat aaatatcatc agcaactatc aaaatatcat tttttactgc ccattcaccg
     6961 attgcctgca gttcattctt gctataaatc attccagtcg gattcgatgg agaattgatg
     7021 attacggctt ttgttttttc tgaacgtgct tgttctagct gctcaacagt aactttccag
     7081 ctattggatt cttccccttt cacaaagaca ggaagtcctt ctgccagttt tacttgctca
     7141 ccgtaactga cccagtaagg cacaggaatg atgacttcat cttgcgggtc aaggatcgtt
     7201 tggaaaaggg tatacaaagc aaatttcgct ccgtctgtta cgactgtttg tgaaggttca
     7261 tagacaagtc catagttttt cttcagataa tcaacgattg cctgtcgaag ctctgggatt
     7321 cctgctgttg gtgtataata gctagctttc ccattgcgga ttgcttcgat tgctgcttct
     7381 tggatgtttt ctggtgtcgc aaaatccggt tctcccacag tcaaactcag cacatctctg
     7441 ccttttgctt ttaatgcctt ggcctttgcc gcagctgcca aagtgacaga cggttctaat
     7501 cgttttgttc tttttgataa ctccatccca ttcattcctt taccttttaa attcccttga
     7561 ttgtcttgac ttcatctcca gattcaaagc caatcaggta ataattcagt ttcccatttg
     7621 tttcttttcc tactacttcc cacacaggct gatcatcata catccctaaa gcagcttttt
     7681 cgatttggat ttcaggatgg gccgcagcaa ttgtctgttt tgcttcgttt tcagtcagtc
     7741 cctttgtttg atccaatact tttacttttt caccagattt aggaatgata acggcgattt
     7801 cttttccttt atcattcgta cccgtcaatg aaaagtacgt tttctttcga gtaaaccagt
     7861 aaaaatgatc ggcttcctcg agatttgcat atttctttgc gatttctact gcttcttttt
     7921 tcgcctggac cgccggacga ctggaacgca agtaaaaaat cgagctgatc acgatgaccg
     7981 ctaataaaaa tacgatcaag ccaagcaaca cgcgattaat cgttttttcc tttgtctgtt
     8041 ttttcttcaa gggtcgtcgc tcctttatct tcattccttt tcattatagc agattttaat
     8101 gatgaaaaaa gacggcacct ttaacttcct tcttcatttt tctttaagaa ttcatggata
     8161 tcctgataga tttcatctat agctagctct tttatcggta cttttttcgg taatgcatgg
     8221 atgattcgtg caccatattt tgcagtgact aatctccgat ccaaaataag taatacgcca
     8281 cgatctgtct ccgtccttat caaacgacct aatgcttggc gtaacttcaa agctgcttta
     8341 ggcacggatt cctgataaaa aggattgatc ccctctgatt ccagataagc attcctagct
     8401 ttgaccatcg gacgtaacgg gttgtcgaag ggtaaacgtg tgactacaac aatctgcaaa
     8461 gcatctccag gtaaatctac gccttcccaa aagctgtcag caccaaataa gacgcttttt
     8521 tctgacaaaa tgaagcgttt caaaagtttt tcccgactcc cgccaaatcc ttgagcaaga
     8581 atttcacgtc ctttttctag catagacaaa tgcattttct gatatacctt ttgcaaaaca
     8641 tcatgagaag tgaataaaac aaggatcggc cgatcttctt gttcaatcaa tcctgtgagt
     8701 gctgcttcta aataatcgat atatgcttga ttgctgactt gatggactgc aggtgcttct
     8761 ttcggcacat agattcttgc ctgtttctca taatcataag gagaagcaat cactttaaga
     8821 ggtgctgctt caatgccaag tcgtttagag agatactgtc gattaggtcc gatcttcagt
     8881 gttcctccaa tataaagaat atggtcatat cgaggatacc acacagttga cggcaacata
     8941 gctgcttgaa aatcaaggag atgaaaaatc atctgctgtg ctgtattccc gggaacgaac
     9001 caatggatca aatgatctcg ccagtcttct agccattggt ccatcagcaa gtattgcttg
     9061 tctaattgat caaataagct atataaactt gctaaatcga tccgcatctg gttggtccaa
     9121 ctttcttgga taatctcaaa agtcaagcgc atctgtgatt gtagttgttg gatctcttga
     9181 taaaaaagtt gcagatgatc cattgctgtt ccagattcca gtggcagctg atccaacatt
     9241 tcttttgtca tcatgacttc ttctggatat tgtccgttag gttgcttgct gcttactaag
     9301 ttcattaatc cctctgccac gcaaatctgc gcttcgcata gtgcagagag ttcttctcga
     9361 taaatcgaca accagcgcaa ggcttcatgt tccttcggca aataactttg tatgcggtca
     9421 aataactgat tcggctcttc cagttgctga atttgtttat ggaagtagtt aatacttagt
     9481 cgtttgtcat ttactttttc agcaatttcc ggcaaatgat gcacttcatc aatcaacaaa
     9541 taaggactag ctggaagcaa tggttctttt cgctgtgttt cttgtactaa aaatgcatgg
     9601 ttgactatca aaaaattgct ttgtttgatt ttcttttgca agaaacgcac aaagtcttgt
     9661 ttgtaaaatg actgattcct tgctaaaaac ccagtgcctc tgtgcgcgac atctgcaaag
     9721 aatgcatggt tcagattggt taaatgtaat tcatccaaat caccggtctt cgtttgtgtt
     9781 agccaaacta agacagccat ttggtacaaa gcatattgtt tttgtcccgg cggttccaca
     9841 agtgtacctt taaaacgctg taaatcaata tagtggcgat agcttttgat aatcacaggt
     9901 tgtatcggct gatctagcaa ttgattcagt aatggaatgt ctttttccat gatctgctgc
     9961 tgcaaaacaa gcgaaacggt gctgatgaca gctggttttt caggtgtagc caaataactc
    10021 aaaggcaaca gataaccaat cgttttgccc attcctgtag ctgcttcgat catcaaatct
    10081 tttgattcat cttttgtgaa atggtcgtag acagcattca tcagcctgtt ctgttctttt
    10141 cgatacatca gtgttttacc aaacattttt tcttttgctt gtttttttct tggatatgtc
    10201 cgctcgccat aatggacgga tgtgaatagc tccacttctt ttttctgcaa agctagccca
    10261 tcgatgattt ctaatgagag atcaaggggc tccgggtttt ctttcatttc ttcaagcacg
    10321 tggtaaatga aacgactcgt atccattcct gtatggctac ttaatcgtgc aatctgctct
    10381 aatgtgatga tcggcaattt cctcattcgt tcttcgatca gtaaaagaag ctgacctgtc
    10441 acttcggcat cactatccgc ctgatgagga ttctcatgaa taaagccaag gctttctgac
    10501 aaatcagcta agcgaaaact tggttccgtc ggcaagaaaa tttgtgcaag ttctacggta
    10561 tcgatgcccg gtatcttcaa actaggaagt ccgcatcgca tgagttcatg gttcaaaaaa
    10621 ttataatcaa aataaatatt atgcgcgaca aatatggtgt ctgccaaaag attgtaaatc
    10681 gtttgggcga catcttcaaa ataaggcgct ttttggacgc gttgattcgt gatccctgtt
    10741 aaatgctgga tttgttttga aatcttccgc cctggatgaa tatcgatcga aaaacgtgaa
    10801 gtgatccgac cagcttcaat catgacgcag ccaaattgga tgatccgatc ttcttttgga
    10861 ttcgtcccag tcgtctcgat atcaacgact gcataagttc ttggtttcaa aatatcttcc
    10921 tcttctcctg cctctaatgt tcttttgaaa ttatactaca aagtgcagaa aaatactctc
    10981 atcgcttttt aaggaaaaaa agacaaaaaa gcccagacac ataaatgtct ggacttttat
    11041 ttttaatata ggtaaattaa gctttagtaa cgtttgttgc ttgaggtccg cgttgacctt
    11101 cttcaacgtc gaaagttact gcttggcctt cttctaaagt tttgaatccg tcgccttgga
    11161 tagctgagaa atgaacgaat acatcgtttc cgttttctgc agtgatgaat ccaaatcctt
    11221 tttctgagtt aaaccatttt actgtacctg tttccataat aaaaatcctc cttgtgtatc
    11281 aacacatatt ttgcaattac ttgaatggtg tatatctgga agatgtttat ttgaaacaag
    11341 ctaccacaga ttaccgaaca aaaactacat tcttactata tcataccgtt tacaaaattg
    11401 ccagagtttt tccttatttt tttgtattta ttctcttatt tagaagatat atttgcaaat
    11461 aaacgaacgt tttctcgaaa tcacccctat aagattcaga aaatttctat ttgctatttt
    11521 cacttttgca aaaaatttca atttattatc tgaaaaagga tgaacgaact ttggaattct
    11581 ctaatgatcc acatcatttt ttaactgata aaaattagta tgcgcatcta tccccctctc
    11641 aactgcaagg ttcgtttttt tgagaaaagt atcttcccct cgcaaagcca atgggactaa
    11701 agcacaaaaa taatgagcga gagacaaaag ctagcttttg tctctcgctc aagatgaatc
    11761 caacggctga aacagaggaa actccttctc tcggagctat acacttctat caacgcctga
    11821 ccattcatct actttggtag ttttcactca ataacgtaag atcgcaacta aactcttttc
    11881 gtctggtgca tctttttgtt caaggataaa gacctgacca ccgttcgtga tcacattatc
    11941 tgctagttca ttcagcacct gacggcggtc atattctgtt tctggatctt cgccaaaacc
    12001 atctgctaaa ttagatgtag caatgaataa atgggatatt ttcccttctt ttgctgccgg
    12061 tataatatca acgagttgat caatgaactt tttatcaagc aacttattgt agtcacttgc
    12121 ttctttgaca gctaattctt gcgatagttt ttttgcgcct gcttcaattt cttttaatga
    12181 aagttgtgca ggtgaagctg agatcgcaac atcggaacga taataagggt tcttcgccac
    12241 ttttttgaac atcgtctgat tttctggtaa tgcaaataag aagataggcc atttctctgg
    12301 attttcaaat gtatctttca aatagtcatc gactgcttga taataattga cccaatcaat
    12361 ttcgacttct tcatctttag gattgactcc atggaacgtc gtaccttcac taccatttga
    12421 tgcgtagttc aacccgcctc ctgttagttc agatcctaaa gcagtgacga catctcttgg
    12481 tgcatcttca ggcaaagcca cttctttgac ttgtttattc gttacttcgt acaatttcat
    12541 cgaatcacga ttcaatccta ataaatagta tgaatagttg aaatgtccat ttttgatgat
    12601 cgctaataaa taaggtagat cactcacata atactggtca tctacaggaa tacccaaacg
    12661 gtggataaac acttgttctg gtgatactat gacagctaca ctcgttgtgg cactacgcca
    12721 gaaagacgga tcgtccaaca aactagtaat tttttcttgg aatggagacc aatcatgctc
    12781 agagtatttc ttttcaaagc gctttttcgc ttcctttgca aaattcttga atttgatttg
    12841 atctttttct acgtcttgat gcgcaacatg ggtgtttaac ataatcgtga caaacggtcc
    12901 tttggtacct tctgaagtca attgtgataa taattctttt tctttttttg ccataaacgt
    12961 acccctccta aaaaaatcat ttctttgtat ctgtaaataa agtctaccac atcccatttt
    13021 atcaatacaa taaaacgaat ccctaccaga gaaatacctg atcgattttt agttgtgttg
    13081 tcctacttca tatttttttc atactttcat caattatatg ctcatttcct taacagtagc
    13141 ttcaaactct cttgctatac tataaaaaaa caaaaaagaa cgaggtgatc ttatgcaatt
    13201 attcatgatt tttagtttgg tcgcactctc agcaggcgcc ctttttgcag catcacgcct
    13261 aaccgaaccc gaagttcagc cagtcaaagt tcgtgtcaat gaacccaaaa gataactttc
    13321 tatgattctt cattaataag acttgttctt tataaggttt ttttcag
//
LOCUS       DAYYNQ010000079        13322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000079 DAYYNQ010000000
VERSION     DAYYNQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.079"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2373
                     /locus_tag="ACHBJ1_002157"
     CDS             <1..2373
                     /locus_tag="ACHBJ1_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM9431078.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2434..3894
                     /locus_tag="ACHBJ1_002158"
     CDS             2434..3894
                     /locus_tag="ACHBJ1_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM9431079.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3905..6109
                     /gene="topB"
                     /locus_tag="ACHBJ1_002159"
     CDS             3905..6109
                     /gene="topB"
                     /locus_tag="ACHBJ1_002159"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9431080.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6125..6274
                     /locus_tag="ACHBJ1_002160"
     CDS             6125..6274
                     /locus_tag="ACHBJ1_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431081.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6349..6942
                     /locus_tag="ACHBJ1_002161"
     CDS             6349..6942
                     /locus_tag="ACHBJ1_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM9431082.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6955..7854
                     /locus_tag="ACHBJ1_002162"
     CDS             6955..7854
                     /locus_tag="ACHBJ1_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM9431083.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7857..8354
                     /gene="ssb"
                     /locus_tag="ACHBJ1_002163"
     CDS             7857..8354
                     /gene="ssb"
                     /locus_tag="ACHBJ1_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM9431084.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8720..8980
                     /locus_tag="ACHBJ1_002164"
     CDS             8720..8980
                     /locus_tag="ACHBJ1_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431085.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9425..9631
                     /locus_tag="ACHBJ1_002165"
     CDS             9425..9631
                     /locus_tag="ACHBJ1_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM9431086.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9652..9882
                     /locus_tag="ACHBJ1_002166"
     CDS             9652..9882
                     /locus_tag="ACHBJ1_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431087.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9898..10335
                     /locus_tag="ACHBJ1_002167"
     CDS             9898..10335
                     /locus_tag="ACHBJ1_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431088.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10328..11035
                     /locus_tag="ACHBJ1_002168"
     CDS             10328..11035
                     /locus_tag="ACHBJ1_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431089.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11199..11399
                     /locus_tag="ACHBJ1_002169"
     CDS             11199..11399
                     /locus_tag="ACHBJ1_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431090.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11415..11594
                     /locus_tag="ACHBJ1_002170"
     CDS             11415..11594
                     /locus_tag="ACHBJ1_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431091.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11919..12182
                     /locus_tag="ACHBJ1_002171"
     CDS             11919..12182
                     /locus_tag="ACHBJ1_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM9431092.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12176..12526
                     /locus_tag="ACHBJ1_002172"
     CDS             12176..12526
                     /locus_tag="ACHBJ1_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9431093.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12550..13164)
                     /locus_tag="ACHBJ1_002173"
     CDS             complement(12550..13164)
                     /locus_tag="ACHBJ1_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431094.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4925 a         2200 c         2738 g         3459 t
ORIGIN      
        1 atggcagaag aaaaagaacc actagacaat agtcgtttgg gaaaatccaa aagaaaacta
       61 gtacgattgc aaaatgaatt aaacgaacaa attgaaaaga tgtttgaaca ccaaaaaatg
      121 acgaacggtc aaccaatgaa cgacaagagg aatggccgtt catggtttaa ccagcaagaa
      181 cgaatcgaaa ataaagtcca aagtctgaga gaagagatca agcaacaaga agaatacgtg
      241 gaaaagcttg aaagacaaga agagctaaag gaaatgggct ataacaagta cggcggactg
      301 gatatgacga tcgataatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
      361 gaatcggctt tttcagccgc cacgatccga aaataccaac ggaagttgga aacattagaa
      421 cagctgaaag aaaaatcaga aaaaggaaaa gaaaatatcc tccctgaagt acaagcgatc
      481 attgacagcg gacgagtgac gcaatggaaa aaaaatccaa ccatttattt tttgaaaggc
      541 tatcgaaaag ttgctttaga actgaatgaa aacgggggat ttgaggaatc aaaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctagacca
      661 actgattcta tgaaggagaa agaaaacgat catttctatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cattggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaggaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aaccaatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atacccattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaagggtttt atgagaaagg acgtggcttt gttcgagaaa cagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta tacgattgaa
     1441 aaagtgtttg atatcacaca aacacagtta aaacctgaag actatccgaa aatctttccg
     1501 aaccgtgtct ttgattttca attggataaa aaaggacagt cagaacttca agctgggatt
     1561 caagcggtag ccaacaatat tggaatacaa attcgagata tgacagaaag tgaagtctat
     1621 caaagagaat taggacaagc acgtggggcg tatgttcgta acgaatttac tggcgaagaa
     1681 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagctacaag tattcatgaa
     1741 ttagcccatg caagaatgca caaattttca gaattagata ccgcaactaa agaattgcag
     1801 gcagaaatga cttcttatat tgtgtgcaaa cattttggaa tggacacgtc ggagaaagct
     1861 attccttata ttgcagcatg gacgaagaat ggtcagacgt tggatgataa ggaagctgcg
     1921 gaacgtggga aaatcatgaa tgatgtcagc cgtgtcgcaa atgaattcat tcaaaccatt
     1981 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccaaccagc agaagtgaag
     2041 caggagcata aaaatacttt agaaacgcca gagaataaga atattgtgcc cgttggttca
     2101 ctttggatcg acaagaaaga cgggaaagta atggaagaag atacgggtcg aacgttacat
     2161 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtaatttt aaaaggaaaa
     2221 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     2281 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2341 gaacaacaat tgtcacaaaa agaacgtgta taaaacaagt aaaaaagact atctgaaaaa
     2401 cggatagtct tttttctttt gggaggtttt aaaatgaccg agaaaaaaat gagtcttatt
     2461 gatcgatgta aacagatcga tatagtggat tttgctcgaa acaatggaat ggctgtagtc
     2521 aataaaggac gagattatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2581 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2641 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     2701 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga aacagaaaag
     2761 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2821 gaagaacgaa aactaccaga gtggctgatt gattatgcag aaaaagaagg gttgattgcc
     2881 gaactaaagc caaaatatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
     2941 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
     3001 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
     3061 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gataaaattt
     3121 tttgagagta gtattgattt acttagctat gctgcactga atcgtgatca gttaaacgat
     3181 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
     3241 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
     3301 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agtcgatcca
     3361 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
     3421 gaatacaaaa tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
     3481 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
     3541 aaagtaaatg cgttttttgg ccagcagctt tctgttaacg attcaataga agccattaat
     3601 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag tttgtgaacg cgtggatggt
     3661 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgcgca gattcttttc
     3721 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
     3781 gaagtaaaaa aggactggaa cgatcaattg aagcatgaga tccagcaaca agaaatcaga
     3841 aagcaaaaac gtgccatgtt gtttcaacaa ggcagacaac aagaaaggga gtgaaatcgt
     3901 catgatgaaa accgttattt tagcagaaaa gccgtcacaa gcgaaagctt atgccgatag
     3961 cttctctaaa gccactcgaa aagacgggta ttttgaaatc caagatcgat tgtttcctgg
     4021 tgaaactgtg atcacgtatg gttttggtca tttagtcgaa ttagattcgc ccgatatgta
     4081 cgatgaaaat tggaaacaat ggtcattaga acatttgcct attttcccta cacattatca
     4141 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     4201 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     4261 gatcattcaa caagtaggcg ccgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     4321 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagacgcaa aagagaccta
     4381 tccaaaattt gaagaagcac aagcacgtca gatcgccgat tggttaattg ggatgaacgg
     4441 tagcccactg tatagtttac tcttgcagca aaaaggcata ccaggaagct tttccttagg
     4501 cagagtccaa accccaacgc tttacatgat ttatcaattg caggaaaaga ttagaaattt
     4561 caagaaagaa ccttattttg aaggaaaatc cgaagtaatc gctcaaaatg gcgcatttga
     4621 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     4681 agaaaagggg gttcaattgg gaaaacagcc aggcatgatt cgccaagttg aaaccgagaa
     4741 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     4801 gctcatgaaa gctagtgcca aagacacttt ggaagccatg caaggcttgt atgaagggaa
     4861 atatctaagc tatccgcgaa cggatacccc ttacattacg gaaggggaat atgcgtatct
     4921 gttggatcac ttagacgaat acaagcattt tttgagggct gaagaaattt cgactcctat
     4981 tcatacaccg aatagtaggt acgtgaacaa caagaaagtc caggaacatt acgcaatcat
     5041 tcctacaaaa acagttatga cagaagcgac gtatgaaagt ctgacacctt tacaacaagc
     5101 gatttacgag caagttttaa aaacgacagt ggcgatgttt gcggaaaaat atacctatga
     5161 agaaacaacg atcttgacgc aagtccagca gcttcagtta aaggcaatcg gaaaagtacc
     5221 gatagatcta ggttggaaga agttatttgg caaagagagc gacggaaaag agaaggaaga
     5281 agagccgcta cttcctaaag tgacaaaagg agaaatggtg acggtagatc tacaagtcct
     5341 tgaaaaagaa accaagccac cacaacctta taccgaaggt acgttgatca ctgcgatgaa
     5401 aacagctggg aaaaccgtgg atagtgaaga agcgcaatcc atattgaaag aagtggaagg
     5461 aatcggtaca gaagcgacac gggcgaatat tattgaaacc ttgaaacaaa aggaatatat
     5521 caaggtagag aaaaataagc ttgtggtgac gaataaaggg gttttgcttt gccaagctgt
     5581 ggaaaaagag ccgctgctta cgagtgccga aatgaccgcc aaatgggaaa gctacttgtt
     5641 aaagattgga gaacgaaaag gaacacaaac cacttttcta gccaatatcc aaaagtttgt
     5701 ctctcactta ttggaagtcg tcccaggaca aatccaatcc attgattttg gatcgacgtt
     5761 acaagaagtg aaagcagcca gtgaaaagca agaagccgcg cgtcatttag gaatttgtcc
     5821 aaaatgccag gaacaagaag tcttactcta tcaaaaggtg gcagcttgta cgtctgaagc
     5881 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcga cacagctgaa
     5941 ggagattatt caaaatagcc gaacaagcca accagtgaaa gggttaaaag gtcagaaggg
     6001 ttcgtttgaa gcaacgattg ttttaaaaga agacttcact acaggctttg aattttctga
     6061 aaagaaaaaa acgaacttca agaaaagaac acgacgcacg acaaaataat ggagggttaa
     6121 tcttatgcgt aatacaacac acaaacaaca agaaatcgaa aatcaaaaag ccaaaattcg
     6181 ttcagaacaa gagaaattac gtgccagtaa aagaagactt cagcaattaa agaaccagca
     6241 acggattcaa aaagaaatcg aattagatcg atagacatta accttgttac aaatacacta
     6301 tcatgatata tttaatgtag tgtatttgta acaaaagggg ggaagtaaat gcaagagaaa
     6361 ctgaaagaat tgttggctat tcgcgatgga aatttatcta tgaaggaagc cagaaaatta
     6421 ggaatcgcag caaccactgt acaaagattg gtgaatcaag gaaaattgat caaagttgat
     6481 cgtggttatt atgtagagga tggtcatgga atagatgatt tattttggct tcagcaacgt
     6541 ttttccaggg gaatcttctc tcatgaaacg actttagata tttaccagct ttctacgaat
     6601 atgccgaaat tcattcactt aacttttccc catggatata acgttgatcg ttcaattaca
     6661 aaagagcagc agttacaatt tcatttcgtg aagaaagaag tttatgaact aggaaaagta
     6721 gagggaactt cctttcaagg aaatcctatt actatgtatg ataaagagcg tacactttgt
     6781 gatatttgga atccgagata tgaaattgaa tatgaaatga aattagaagc attaaaagag
     6841 tacatggaag acccattgag agatccaagt aaattaagag attacatgac aaaactacat
     6901 gtagaaaaag aaatgaaata ccacatgcaa agcttatatt gatggaggaa aaagatgaca
     6961 ccgacacaat ttgcagcaaa aacaagaaat ttagcaaaga ggacaggatt aaatgcacag
     7021 ttagtacaac ggcattttat gatggatcgt ttaattgaac aaatttctga atctaagtat
     7081 aaaaatgatt tcattttgaa aggtggcttt ttattaggat caaaatatgg aatcgataga
     7141 aggacgacaa ttgatatcga cacgacattt agaaatagca aattaactga agaaaaattg
     7201 aagagtattt ttaatgattt aactaggaaa cccacgaaag aaggtattgt tttctctatt
     7261 caaggaatga aggaaacaag agaagccgat ttttatcctg gatttcaaat taaattggtg
     7321 gctaatttag agaaagtacg tgtgccattt aaaatggacg tgacaacagg agattctatt
     7381 tatcctgaag tagatacaca ctatcaccaa ttaatgtttg aagataaaaa ggtggagatt
     7441 ccagcttatc caacagcaca aattattgca gaaaagctaa gtgctacgtt cagctttggc
     7501 acagataatt cgagagcaaa agattactac gacttattta ccattccgaa actagaaaat
     7561 atcgatacga aagagttata caaaagtgtt cgaaatacat ttacaaaacg tggggataca
     7621 aaaccgttgt ccttttatta tgaaagagaa atggataaga tcataggaaa tgactatttg
     7681 gaaaagcagt gggaaaaata tcgctcagtt aacacgtttg cgaattccat ttcatttaca
     7741 gatacagtca acgaaattga tcacctaatg aaaagtgtga tcaaaattga aaatcaagaa
     7801 agaaaacggc agtttaaaag atcacaacaa atgaatcaag aaatggagag ataaagatga
     7861 tcaataatgt aactttagta ggacgcttaa ccaaagatcc agatttacgt tataccgcga
     7921 gtggtactgc agtagcgact tttaccctag ctgtcaaccg aaatttcacg aatcaaaacg
     7981 gcaatcgaga agctgatttt attaactgtg tgatttggcg caaaccagca gaaacaatgg
     8041 tgacattagc taaaaaaggt agcttgattg gtgtagttgg gcggattcaa acgcggactt
     8101 atgacaacca gcaaggacaa cgtgtgtatg tgacagaagt tgtggccgat aatttccaac
     8161 tattagaaag caaagcagcg acagaaagcc gtgcgcacgc tgatcaatcg tccaccagtc
     8221 caagcaccac cacttttgaa caacgggata cagcgacgcc taataacaat ggattgaacg
     8281 ctttacagaa tccatttggg aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cgcgatcgat gggcatataa aaaaagaaga gttgcaatcc
     9001 taatgtagga ctgcagctct ttttgtttta catagaaaca agaaaaatga aaaggccttt
     9061 ttaggaaggc gcgagcgcct tctcaattga aaaacctctc tcactccaac cgccaccgcc
     9121 gttcgagaag aaaggaagtg aacgttgcac gttcactaag ataaagggtc ttgcaaagca
     9181 agacaaaata gaaaaaactt cctctcacca aaaggttcgt tcctgttcgt tatctcttta
     9241 ttgatttgcc agaccggcaa caagttgcct tttatcacca aaaagcgaca aggacaaggg
     9301 taaatggcac gctgcgcgcc cttgtccttg tcgcttttcg ttgattcatt ctggccaaca
     9361 aatcaataaa gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc
     9421 aatcatgcga aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta
     9481 tcatgcacca catactgtcg aatgcgattg tggacaaaga gcgaaatgta tcaaaagcta
     9541 tcgtttgtat caatgtccaa catgcggaaa aaaatacacc ttgtcttatg gcgattatat
     9601 tttgattgat gagaaaggaa agaaacgctg atgaaaacat aacataaatt attgactgta
     9661 aaagaagtcg ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagct
     9721 ttacttattc cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg
     9781 ctatttgaag acctagtgaa gattgcagga tatgaactgc attatcaaga aaacatggaa
     9841 acttatgaaa acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg
     9901 gctcaaaatt actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg
     9961 gaggatatga cattcctata ccatgagaca cgtgttccaa aaaaacatta taaggaaaga
    10021 ttgtcggtag ctgtggaaga aatgattgaa tcaggtgtcg aaatgaatct cattgcgacg
    10081 tactatcgaa cactggaaga actgaaaaaa caaaatgcaa aatggttttt ccaagcctta
    10141 ctatgcttgg aagtaggtgt caaaccaagc actattaaac cttctgaata tcaagcttta
    10201 gaactaacgt atgggaaatt tgtcgaaacg aaaaaagcca agactgtttc aagtgaatgg
    10261 ttagattatt ttgaaaatat caataagtat ggcgcctatt acaccatgaa aaaggaggac
    10321 aacgaaaatg aataaggtga tttatcaact aacaacggaa gatgtgtcac actttttaac
    10381 aaacaaagaa catttgaatg aagtaatcag acggattcaa aaagacttta gttgtatgga
    10441 agaaatgaag tactttattc aaagagtcga agaacaattg tatttggaaa gtgaaacgag
    10501 tcaaacgaag gtcacagtca gcaataatta tttagacgtg tataaacatc gtcctgaaac
    10561 ggtaaaaaaa ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta
    10621 cctagaaagg agtgaactgg tcgaaaactt tgtcagctgc acgatttgca ggaaacacta
    10681 ttactcagaa tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat
    10741 ggaagagcta aacagcaaaa atgcgacgat agacgattat tatcttgtat ttactagatt
    10801 tttgacgtgt ggaacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc
    10861 cgaagcattt ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac
    10921 gactttaact gattttttag atacctacac ttacgacgat acagaagaaa tccaaaatga
    10981 acttgaccgt aaacgaatgc catatcaacg atttgaaagg aaaaaaaggc aataatcatt
    11041 tataccgaaa agtgagaatg tgtgaaataa gaacaaaaac cttgaaatta agggaatggt
    11101 tcttatttcg caccaatcgc tccaaccgcc cccgccgctt aggtggtgcg aaataagcgg
    11161 gcaaagcccg ataacctaag agaaagaagg agatccatat gaataaaatc attcctctgt
    11221 tgttcgtact gtgtattggc gagatactta tgcgtcatgg tgcagctgta catttaaact
    11281 tcgtagtatt cttaggaata ctagtatttt gtttgatttt ttggcgcttt gtgttagccc
    11341 ttgtgagtct actattcaag ttgattgcag ccatattttt catagggtta tttcgatgaa
    11401 aggagagagt actaatgcca attgcaatag gaaataaacg gttacccgtg acattagatg
    11461 aaaagagaca aaaagaattg cagcaactaa agcagaagta cggaaaaagt gaatctaaga
    11521 ttatgtgtat tgcgttagat ctattgatcg cgcaagaaaa agcaggattt gatataccag
    11581 cactaaaaaa gtgacgtcac ttttcatttg aagaaacaaa agtgatagca cttaattgtt
    11641 cctactattt agaatatttt ctatgtcctt tttattccat tttgtatcaa taactatgaa
    11701 atctttaaag tatccagatc aatttttttc ttacctgatt ctaaagattt tgttttctta
    11761 aacatattat gatttccttt cgactatttt catataaatg ttaacatagt ttatggattc
    11821 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
    11881 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
    11941 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
    12001 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
    12061 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
    12121 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
    12181 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
    12241 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
    12301 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
    12361 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
    12421 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
    12481 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
    12541 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
    12601 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
    12661 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
    12721 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
    12781 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
    12841 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
    12901 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
    12961 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
    13021 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
    13081 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
    13141 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
    13201 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
    13261 taccttatat agacaaaaac taatgatgga ggaaatagta tgtgtcactt ttctttttaa
    13321 at
//
LOCUS       DAYYNQ010000080        13219 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000080 DAYYNQ010000000
VERSION     DAYYNQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.080"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1420)
                     /locus_tag="ACHBJ1_002174"
     CDS             complement(<1..1420)
                     /locus_tag="ACHBJ1_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM9431095.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1672..2853
                     /locus_tag="ACHBJ1_002175"
     CDS             1672..2853
                     /locus_tag="ACHBJ1_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9431096.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHBJ1_002176"
     CDS             complement(2913..4541)
                     /gene="treC"
                     /locus_tag="ACHBJ1_002176"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM9431097.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4992..7703)
                     /locus_tag="ACHBJ1_002177"
     CDS             complement(4992..7703)
                     /locus_tag="ACHBJ1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM9431098.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7713..8414)
                     /locus_tag="ACHBJ1_002178"
     CDS             complement(7713..8414)
                     /locus_tag="ACHBJ1_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431099.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8600..9199
                     /locus_tag="ACHBJ1_002179"
     CDS             8600..9199
                     /locus_tag="ACHBJ1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM9431100.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9280..10293
                     /locus_tag="ACHBJ1_002180"
     CDS             9280..10293
                     /locus_tag="ACHBJ1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM9431101.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHBJ1_002181"
     CDS             10401..11318
                     /gene="rbsK"
                     /locus_tag="ACHBJ1_002181"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM9431102.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHBJ1_002182"
     CDS             11346..11741
                     /gene="rbsD"
                     /locus_tag="ACHBJ1_002182"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM9431103.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11741..13114
                     /locus_tag="ACHBJ1_002183"
     CDS             11741..13114
                     /locus_tag="ACHBJ1_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9431104.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3760 a         2618 c         2450 g         4391 t
ORIGIN      
        1 ttcctgcata taaatgaaaa tgttcggagt cagccggtgc aatcgtgtga tcactgaatt
       61 gaatatatct aaaagcgcca ctttccttat cttctgcttc gaatagatac cttacgccac
      121 ggtttcctgc ctcgtaggtc aagatttctt tccctacata acgataattt gaacgtttcg
      181 ttgttccatt cttaaacgta aatgccatct ctttctcact gatcttgatt gaagcaatat
      241 ctgtttgata gcccttcgta taatattctt tatattcttg tgctgtctta tcctgattca
      301 atttttcttt atattcaaaa acaggatcaa aagtcccatc ttctacatac gtatctactg
      361 tttgccaagt tcctgcccaa tctgaaagag agcgatcttg tacatcctcg tcttcaaaat
      421 aaccgttgta gacgctcttt tcttttttgg gggtcaaagt atcttctagc gggttttctg
      481 tctctgtcgt ttttttcaac gcttcgaggt ttgcttccat gatagaaaga tagttttctc
      541 cattttctat ttgttcattc gtcaatcctt ccaaaggatt caatacttct aaagatacac
      601 ctgcttcgtt tgctaacgtt ctggcaatac tatctttggc gttttcttca aaataaatat
      661 agttgattcc atgttcttta acaaagtgtt tcagttcagc aattctcgcc gcagacggtt
      721 cttcactaga agaaagacca gcaatcgata cttgattcaa tccatattct aacgctagat
      781 agttgaatgc agcatgttgc gtgacaaaag atctttgttt tgcttgctta aaggtttctt
      841 gataaagctg atggagtgct tgaagtttct cggtataggc tgctgcattt tttgtccata
      901 cttcctgttt ttctgggtaa gcttcaatca gctgatcacg tatgtttgct acctgcttga
      961 ttgctaaact tggagctagc catacatgtg ggtctagctc atgtgtatgg ccttcttccc
     1021 cgtgttcatg gtcatgatca tgtccttctt cacttcccgg aagtaaaagc ataccttctg
     1081 tcgcttttat cgtatggaca ttgccttctt gtaatgtttt ttggattgcg gggacccatg
     1141 tttccatgtt ttcatcatta taaacaaaga tatctgtatc ttggatcttt gccatgtctt
     1201 tagcagaagg ttcataatcg tgggaatcag tccctgctgg aatcaataac tctacttctc
     1261 cttcatcccc gacgattgct tttgtaaaat catacatggg ataaaaggtc gtcatcacgc
     1321 tcaatttttc tttactgtcc tcggctttcc cacttgcccc gcaagcagtt aacatacctg
     1381 ctaaaaccag catggccaat atacctatca atcgtttcat ttatgacact cctcttctca
     1441 tcttctttca tttgaatgtt ccgattcgct tttcgcaaac cgtaatagtt acgttttagc
     1501 aacaggattt aatttatcat atggttaaaa gaactgtcaa gcgttttctt attttaattg
     1561 atgagaaagt ttatatgaat gattgacgat atttttgcaa tcaacctcta tcattttcct
     1621 tttatacccg cttttgtcta taatgaagtt atagataaag gaggaaaaag agtgaaaaaa
     1681 gaaacagata caacgaatcg tttgatccgc tttttaggag gaaaaacctc ttactatgtt
     1741 ttaggaatag ttatcttact tgcgctgatc atttattttt tccatcagat ctcgtttata
     1801 ttcaagcctg tcggaatcat tatcgctact atcttgcccc cattattgtt tgcactgatc
     1861 ttgtattatc ttttggaacc agtcatcaaa cgtttgagca agcacctttc gaggaacttg
     1921 tcagtcatta tcgtttatgt gattatttta tttttattgg ggcttggaag catatggctt
     1981 attccttttc ttgaagaaca ggcaaagaca ttgatcagta gtttgccaga tttgtttcaa
     2041 gattttcaag agagtctccg acaatttttg gagaaaacac cttttgccga ttcatttaat
     2101 caatttttcg cttctattga cgacgtgacg tctgatatag ctggatttat tggaaattat
     2161 tgggaaagtg gcgcacaacg gatcagcaac attttttcaa ctgttacagc tatttttatt
     2221 actttattta ctggtccaat tgtcgctttt ttccttttga gagatcctca aaaattttat
     2281 cagtcagttt tagcgattgt cccaccagca tttcgtacag attttaaaaa tttgacaaag
     2341 attgctaatc aacaattggg ttcatttcta aaggggcaaa tcattgcttc atttatcctt
     2401 ggagcagtct actgggtttg tttcttactg atcggattag aatttgctag tgttttagct
     2461 atttctgctg ggttgttatg tatcattcca tatattgggc cgtttatcgc cttctttcca
     2521 ggtttgatca ttgcattcca agactctact tttatggctg tcaaatttgt cattgtctgg
     2581 tttgccgtac aacttctcca cggtgacttg gtgattcctc gagtaatggg tgatcgttta
     2641 cagatccatc cgattacgat cttaatcgtt ttacttgtca tgggtgatct aatgggaatt
     2701 gtgggcgtta tttttggtat tcctatctat acgttagtca aattactcgt tacttttatt
     2761 ttccgtaaat tcaaacaacg ctacaataaa ttttatggcg ataaaggaga gtatgaaaac
     2821 tcttcatttt ctgaagaaga ttattttaaa taaaacacaa aacgtcgatc ttacgagaat
     2881 agctcgtagg aacgacgttt tgtgtttttg ttttaatttt tcagcaaagc aattgcctgg
     2941 tatggtttta gctccagact ttgatgaaga acagtatcgg aataattatc gataagaata
     3001 ttggcttctg tgaatccttc tgggatggcg atccttgtat ctttatcata gaaatttgtt
     3061 aatacaagca gctgctgtcc ttcaaattgg cgaagaaagc cataaacttc cggatgattt
     3121 gcttcataag catgataatc tccttgtgca acgattggat atgtttttct cagccgaatc
     3181 aatttcttgt aataggacaa aatagaatct tcagaagata gttctttttc tacattaatc
     3241 tcttctagct tgcctccaac ttttaaccaa ggtttccctg tagtaaatcc tgcttgttct
     3301 gtgtctgacc attgcatggg tgttcgagag ttatcgcgag attttgcttt gatccgacga
     3361 aacgcttctt tttctgtgaa tccttgttca aggagtaact tataagcatt caaactttct
     3421 acgtcaatat agtcggaaat cgatgaatag tctgggtcga tcatgccgat ttcttctccc
     3481 atataaatat agggcgtccc acggttcaaa tggatcatgg ttgctagcat ctctgctcct
     3541 tgttttcgat actgatcgat agctacaaat cgattgagcg cacgtggctg atcatgatta
     3601 ttaaaaaata gagcattcca gccgttaccc tcactcatct tttctcccca tgtatgaaat
     3661 agtcgcttta attctgcaaa atcaaatgga ggagtagacc atttattccc attttcataa
     3721 tcgactttca aatgatggaa attaaagacc attgataatt cttctctact aggttctgta
     3781 tacaagatac aattttcgat cgtcgtagag ctcatttcac cgactgtgat gatagtggaa
     3841 tccgtcccaa acgttgcttg gttcaattct tttagatacg tatgggtgat tggtttgtcc
     3901 gtatattcag gtttgccatc attttcttta ttatttttca attgttcgtc tttaccgata
     3961 acattgataa catcgaaacg gaatcctttt accccttttt ccatccaaaa acgaacaact
     4021 ttgaatagtt cctctcttac tgcttggttc cgccagttca agtcagcttg tgtccgatca
     4081 tataagtgca aataatattt ccccgtatct ccaaatggcg accaagcact tccaccaaat
     4141 tttgataccc aatccgttgg ctcttctctt aaaatgaaga agtcctgata atgctggtcc
     4201 cctgctagtg cttttttgaa ccattcatgt tctgtcgaaa cgtgattcag caccatatcg
     4261 agcatgattt ctatccggta tttctttgct tcggctatta gctgttcgaa ttcctccatc
     4321 gtaccaaaga taggatcgat ggctgtgtaa tcagagatat cataaccgtt gtctttttga
     4381 gggcttggat aaatagggtt tagccaaatc atgtcgatgc ctagatcatt cagatacggg
     4441 agtttttctt tgattccttt taaatctcca attccatcac cattactgtc tttgtaagat
     4501 ttgggataga tttggtaaat gactttgtct ttaaatgtca tggatattta cctccattca
     4561 actgatttat tacctaccac aacttatata tacaagttat catttattat tataactgaa
     4621 ccgaaagcgt ttgtaaaatt tatctttata cattctaaga ggtctgttgc ccaataaaaa
     4681 acaagagatc agacatgtat ttgtctatcc tcttgtttaa aatagcaaaa tattcttgtt
     4741 tcttctataa atggtgttca aaagctttta tctacggcaa tcagtcttct caaatgtaaa
     4801 acatgaggag ctaattttat ttgagtattc tttttgctcg atgcaggacg gcttttttac
     4861 aacctctgat tcatggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
     4921 atatgaaaaa ctattttgcc gtttgtttct ctaatactca ggtcaaaccg ctgatttcac
     4981 aacctctgtt tttattcatt cgatttcaac gcatcaatca tatcgatatg ctttaatttg
     5041 aaatgggtaa caatcatcac aataaccgta aatacgatag ttaatcctgc tgataagaca
     5101 taagcccacc atgtgatcac taatggaaag atgacattct ccatagacgc ttgttgcaag
     5161 atgaagtcag tcaatatata tcctacacca aatcctccga tgattcctaa aagagtaaag
     5221 atgatatttt ctcgaacaat gtacatcgtg acttctttat caaaaaatcc aaggactttt
     5281 attgtagata attcgcgtac acgctcggaa atattgatat tagttagatt atacaagacg
     5341 ataaaagcca gcagtcctga taatacgaca aagattaaga caaccgagtc taagttcgcc
     5401 attgattctt cttgcgtttc gatttgtttg gacatgaaag acgtatttgt cacagcgtct
     5461 gtggcaagca gtttctcgga aagcatttct tcttcttttg ttgtcatctt atctgtacgc
     5521 agcaaaaatg cattatccat catcgattga tcagtaactg aagcataata ttcaggggac
     5581 atataaataa agctacctag atagtttttc acgatttctt ttacttcgac tttccattct
     5641 ttttgatctt catcataaag cgaaatatga tctcctgtag ataagtcaaa tagttctgct
     5701 actttttgcg taatagcgat tcctgtttta ggcaaggagc gtgggttctc ttcaagatca
     5761 gttaaatgga taaaacgatc caaatcagct gttttacttg gtacgatcac tgtcagactt
     5821 tgcgaagcat gtcctttttt tctcaattct acggtttgag tatgaatagc taggatctca
     5881 tttacttttt cttgcttctg cagcatatcc gctgcttttt gacgactatc ttccgtggat
     5941 gaatccaatg taacgattgc ttgatagtca atgatcggtc caaattgttt ggctgaaaca
     6001 gaacttagag aatctttcaa tcccacgcct gcaaccatca gcccggcaca tccagcaatt
     6061 ccgataatcg ccataagcat tcttgactta tatcggaaga gatttcggta actgattttt
     6121 tgattaaagc taaggcgtga ccacagcgga gtgatgtatt caagaaaaat gcgtttccct
     6181 ggcttcggtg ctttcggttg aagcaatgca gcaggccgtt ctcttaaatc tttgaataaa
     6241 acgatcattg cagcgccaaa tgcggcaatg aagaatgcgg cagctgcttg taaaatcggc
     6301 ggccagtcat aaaaaataag cgcactccca atatcatacc gctcgttaga cagctcaaaa
     6361 atgatccttg gcaaaagatt cgttcctaat atcgttccta aaacaatacc tatccctgag
     6421 gctagacttg catagattgc atattttttt gcgatttcaa ctttcgtata acctaacgcc
     6481 tttaatgtcc cgatttccct gcggttttct tctaccatac gtgtcattgt cgtaaaggtg
     6541 atcaatgcgg caataaagaa aaagaaaaca ggaaaaacat tggcaatcgc agcgatacga
     6601 tcagacagat caccaaattc ttgaaaaccg acattttctg aacgttcatt gtataggtat
     6661 gatggtttag ttaaattttc aattttttct gcatttcgat tcaattcttc ctctgcagag
     6721 gccagttcac gttctttctc aactaattgc ctttcttgtt cagcaagttg agcttcttgt
     6781 tgagataatg tttgaacagc gattgttcgt tgcgcttcag gaagctgctg gatagttgct
     6841 gactgttgcg ctaactgttt tttagcatct tctaaccgtg ttcttgcttg cgttgtttgt
     6901 gctttgccat ccgctatttt ttgctgtgcc ggttctaatt cttcgttggc cgattttttc
     6961 agctcttcta agcgttcttc gggtcgatct tgtaaatact tttctgcctt ttcttgattt
     7021 tgctccattt tatctttgta agctttgctg tacgtattca aattctttac gtttgtaaaa
     7081 tcaagatata agacagaata aatatctgtt ttaaaaacgg attcaggtaa atagacaaaa
     7141 taatcgacgc taccatttcc gacgttcgcg tatcctctct ccaaattgtt gatgaacaat
     7201 ggagaacgga caaagcccgt gatggtatac tctttttctt tcaattcatc tgaatcaatc
     7261 tgataaatat caccgatttt atacccttca tcttccgctt tttcatccaa tacaagctgg
     7321 tttggttttt ccgggagttt tccatcagtt aattgtagct tgttctgctt gttatttttg
     7381 ttataagaaa atatttgaat cacttcgttt ttgccagtat tggaataata gatctgtttt
     7441 cctgtttcta cttttgcatc tgggatcttt tctgcttgag caatatcttt ttgtgtcaag
     7501 ccaccagtag aaacgatttg caaatcagaa agtcctgctg taccaacata gtgatccatc
     7561 gttttttgca tgacaggtcc agcagttttg acacctacaa ataaaagtgt tcctagcatg
     7621 acaataagga tgattgcaat gaatcgtcct ttagattgcc gaatttcgcg aaaacctgct
     7681 ttaagatatg ttcttgtttt cataaaatcc ccctaccata caatctcatc aatggatgaa
     7741 ggatgatcat ttaattcaac agaccttact ttagcatcat taatgtgaat gactctatca
     7801 gcgatgggcg ctaaagcaga attatgtgta atgatcaaaa cggtattgcc gtgttgtctg
     7861 ctagcgtttt gcaataattt caatacttgc ttgcccgttt caaaatccag cgcgcctgtc
     7921 ggttcatcac ataaaagaag tttgggattt ttagctagtg cacgagcaat tgaaaccctc
     7981 tgctgttcgc ctcctgataa ttgggacgga aagttattca atcgatgagc taggcccact
     8041 tgcctcaata cctctacagg atcaagcgca tcaggagaaa cttccgttgc caattccaca
     8101 ttttcttttg ctgttagatt aggaacgaga ttgtagaatt ggaaaacgaa ccctacatct
     8161 gttcgtcggt aagcagtaag ctgtttatct gaaaattgcg caatatctgt gtcatcaata
     8221 atgatctgac cttcatcagg tgtatccatc ccacctaaaa tattaagaat cgttgacttt
     8281 cctgctccac ttggaccaag tataatcact aattcgcctt tttcaattga aaaagaaata
     8341 tcgtcatttg ctgtaatggt cgtttctccc attttgtatc gtttgtattc attttttact
     8401 tcgacatact tcactctgat tcctccattt tcacgatatt tctcgctata tctatgcatc
     8461 gaatgcgttt tctcacactt atttatttta ccactgaatt tctctagact aaagaaagga
     8521 atcctttaat ttagttgaca aataaacgat ttatgattta cttacataaa aaacattgtt
     8581 atttgaaaga aggaacatca tgaatgattt aaaggaaaat atgatttttg atttatcaaa
     8641 aactgctttt gtcgcgatcg acatgcaaaa agagatcgta aataacggta cactctctcc
     8701 acacactgca gcatctattc tgacaaacaa tgatttgtta gtaagaacat tgaaacgtac
     8761 cgctgccttg aaagttttag tgaacgtaga catctccact ttcccttatt tgagtcaaca
     8821 aactgatatg ggtggaatga ctgcttctgt acctccagaa ttcactgatt tacttttgaa
     8881 ggattctttg aaagatacag ataatatgtt gactatcaca aaatacaatc caagtgcttt
     8941 ttttggtact tctcttgatc ttcaacttcg ccggcgtgga atcgaaacta tcattttaag
     9001 cggtgtagcg acaacaaacg gtgtatatgc tactgcatta gatgcctttc aacacgggta
     9061 ccatatcgtg cttgctgaag atgcttgcag tgatcgtgac aaagaatccc atcaattgtt
     9121 cataaaaaag atatttccga aaactgcacg agtacgctca acgaaacaaa taatcgaagc
     9181 tatccaacaa agcaaataag tactggcaca cgtgattttc atttacttct ttttgatttc
     9241 atgggaaaat aaccacagga tttagatagg agtatgaaca tggtgaaaaa aaagatcaca
     9301 atcaaagaag tcgctaaact ttccggcgtt tcaatcacaa ctgtctcctt gatattaaat
     9361 ggaaatacgg agaaattcag tccgaaaact gttcagaaag ttttagctgc caaagaagaa
     9421 ttaggctatc agcctgacta ttttgctaga caaatgatta ccaaggaaac gaaaacaatt
     9481 ggtgtactcg ttcctgatat cacgaatcca ttcttcagta cactgatgcg aggaattgag
     9541 gatatcttgt ataagcagaa tttcgtcacg atattatgta atgctgattc tgatcatcaa
     9601 aaagaaatcg aatatctagc cgagctcaca cgtcgaggtg tggatggatt cattatcgcg
     9661 acttctgctg tctctacaga tgcaatcaac gaaaatttga aaaaacaagg ccggcccttc
     9721 attgttttgg atcagaaaaa atctgaagga tttagtgatg ccgtacgaac agacgatttt
     9781 cgcggtggtt acctagcggg catgcacttg cttagtctag gacatcaaac gatcgccctt
     9841 gtctatcctg aaaatccacc ggaaaatgtc cacgctcgga ttgaggggtt taaatctgct
     9901 cttgatgttt accaaatccc tcacgaccaa ctgatcctct tacctacaca gttctcaaaa
     9961 caaggtggct accagatcac agcagaactt cttgattctg ctgcaactgg tgtgtttgct
    10021 ttaaatgatg aattagcttt tggattatat cgcggattag aggaagctgg aaaaagcatc
    10081 cctgaagact acagcattat tggctatgat aatatagata tgtgcgagta tatcaagccg
    10141 aaactgacaa cgatcgcaca accaatcttc gaattgggac aaacatctgc caagttattg
    10201 ttagatcgga tccaattccc agaaaaagaa tgggaagaaa aaagattacc tgtacgatta
    10261 gaaaaaagat tttcaactgc acccttgaaa tagctttcaa ggtgtgctat atttttatcg
    10321 aactattaaa acgttttact aaaacgtttt aaaaaataat tttcccatca aaataaatga
    10381 gatagttgga ggtttgtaaa atgaacacgg ttacagtaat tggaagtatt aatatggaca
    10441 ctacccttcg tttaaccaac atgcccaagc caggcgaaac gatgcatgcg catgagattt
    10501 ttcacgctgg cggaggtaaa ggtgccaacc aagcagtggc agccaaacgt tcaggcgcac
    10561 ggacatcttt tattggaggc gttggggctg acagcgaagg acaacagtta ttagatctac
    10621 tgacaaaaga aaacatcgat acatctggga tagctgaaat ccaaggcgcc acaaccggac
    10681 aagcaatgat tatggtagac gcagcaggag aaaatagtat cttgatccat gcaggggcaa
    10741 ataatgcttt ccatgaacaa gaagtcctaa aaaacaagca actgattaca aacagtgact
    10801 ttattattgc tcagtttgaa agtagcttag atgcgacgat cctagctttt tctattgcaa
    10861 aagacgcagg aaaaacaacg atcctgaatc ctgcaccagc tcgtgaaacg attcctactg
    10921 aactacttga aaagacagat atcattatac caaatgaaac agaaacagaa atcattacag
    10981 gaatcagagt gacagatcat aacagtttgg tcgcagcagc cgaaaaactc catgaattag
    11041 gtattggaac agtaatcatt acactaggca gtgccggcgc tttctatcat acagaaaaag
    11101 aacacggtat cgtccctgct tttaaagtgg atgccgttga cacaacagcc gcaggtgata
    11161 catttattgg cgcattaagc agcacacttc agccagacct tagtaacttg aaagaagcga
    11221 tcctttatgg aaatctagcc tcttctgttg ctgtgcagtc ttatggtgcg caaccttcta
    11281 tcccttaccg cgaagcttta gaacaatcag cagtctaaaa ttaacaatga gaggaagaaa
    11341 caaacatgaa aaaaggaaaa gtaatcaatt cagatatttc acgagtaatc gcacaaatgg
    11401 ggcactttga taaattaagt atcggggatg caggcatgcc tgtccccttt ggtactgaaa
    11461 aaatagattt ggctgtctca aacggtcttc caagtttttt atctgtttta gaaaacgttt
    11521 tagaagaact tgaggtacaa tcgattgaat tggcggaaga aataaagaca atgaacccgg
    11581 aaatattgga aaaaatcaaa ttattgttac cagatacacc gattagcttt atcccacatg
    11641 aagaaatgaa aaaagagttg aataactgtc atgcatttat tcgtacaggc gaaatgacac
    11701 cttacgccaa tattatatta gtgagtggag tggtcttcta atggaaacga caatgaatcc
    11761 tgaacagatg gtactgtccg agcagattca tatgctgatt gaacagttgg gaggaaaaga
    11821 caatattgat tctgtccttc catgtgcgac tcggcttcgt atcaaattga aaaaacagcc
    11881 agtagccaat gcagaaaaat taaaacagca aatcccttcc attaagtatt tcattttact
    11941 cgatagatat gcagaaataa tcatggatca gggattggct cctgcttaca aggaattgac
    12001 gcgtcaaatc gatcattcag ctccgaaaga aaagctaaat ccgatcgaaa aactatttgg
    12061 tttgatatcg gaaatcttta cgccgatcat tccgctgttt gccggttctg gtatattaaa
    12121 aggtctggtt gttttagcga ctacgattgg ctggcttagt gaaacaagcg gaacttatca
    12181 tattttgtct gctgcgtcga atgccgtctt ttattttctg ccaatctttc ttgctttttc
    12241 tgctgccaaa acgtttaaag tgaatggata tattgcggct gtcattgctg cagcactgat
    12301 tgaaccgaat ctgacctctt tactttcaga cagtggcaaa gcagctactg attttctggg
    12361 gattcctgta gtactgatgc agtatacgtc tactgtcatg ccggcgattt taggtatttt
    12421 cttttattct tttgttgaaa aattcctcaa aaaatatatt cgcgaaaata tgcaattagt
    12481 atttgtacct ttactttcat tagttatcac ggttcctttg acgttaatgg tattcgggcc
    12541 tgcgggtgtt taccttggcg aaggtttagc tgccgcaatt acttggatga tggatattaa
    12601 cggaccgatc agtggcgcaa tcatcgctgg tggttggaat attttggtaa tatttggtat
    12661 ccaatgggct gtaaaccctg tcatgatcag taatatctct gcttatgggt tcgaccgaat
    12721 cgttccgttg acaggagcag ccaattttgg aatggctggt gcagctttag gtgtatttct
    12781 gcgaagtaaa cgttcaaaaa cccgttcgat ctccggttct gcttttgctt cgattttact
    12841 tgcgggagtg accgaaccaa ctgtttacgg aattgcgatc ccattgaaaa aaccatttgt
    12901 tgctgcttgc attggtgcag ctgctggtgg agctgtaatg ggatttgccc aagtgaaagc
    12961 gatcgctttt gtatttggta gtctgaccac attgcctgca ttcatttccg gtactttttt
    13021 ctggtactta gcaggactgg ctgttagtct agtcgtagca atgatcacta ctctcgttag
    13081 cgggtttgat gaagacctaa tgtcttatga atagttaatg aaaaagactg aagtatgaga
    13141 tgtttatcac atatttcagt ctttttcttt ttaggatcat tatttttata aatttttgtg
    13201 atactttcca aactttggt
//
LOCUS       DAYYNQ010000081        13104 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000081 DAYYNQ010000000
VERSION     DAYYNQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.081"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACHBJ1_002184"
     CDS             complement(<1..914)
                     /locus_tag="ACHBJ1_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM9431105.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACHBJ1_002185"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACHBJ1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM9431106.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACHBJ1_002186"
     CDS             2006..2176
                     /locus_tag="ACHBJ1_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431107.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACHBJ1_002187"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACHBJ1_002187"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM9431108.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACHBJ1_002188"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACHBJ1_002188"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM9431109.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACHBJ1_002189"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACHBJ1_002189"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM9431110.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACHBJ1_002190"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACHBJ1_002190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM9431111.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACHBJ1_002191"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACHBJ1_002191"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM9431112.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACHBJ1_002192"
     CDS             6751..7752
                     /locus_tag="ACHBJ1_002192"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM9431113.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACHBJ1_002193"
     CDS             7749..9110
                     /locus_tag="ACHBJ1_002193"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM9431114.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACHBJ1_002194"
     CDS             9070..10221
                     /locus_tag="ACHBJ1_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM9431115.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACHBJ1_002195"
     CDS             10196..11365
                     /locus_tag="ACHBJ1_002195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9431116.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACHBJ1_002196"
     CDS             11540..12919
                     /locus_tag="ACHBJ1_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM9431117.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4365 a         2290 c         2854 g         3595 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaa
//
LOCUS       DAYYNQ010000082        13084 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000082 DAYYNQ010000000
VERSION     DAYYNQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.082"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACHBJ1_002197"
     CDS             complement(79..2199)
                     /locus_tag="ACHBJ1_002197"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM9431118.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACHBJ1_002198"
     CDS             2429..3607
                     /locus_tag="ACHBJ1_002198"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM9431119.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBJ1_002199"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACHBJ1_002199"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM9431120.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBJ1_002200"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACHBJ1_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM9431121.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACHBJ1_002201"
     CDS             complement(4960..6213)
                     /locus_tag="ACHBJ1_002201"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9431122.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBJ1_002202"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACHBJ1_002202"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM9431123.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACHBJ1_002203"
     CDS             7790..9136
                     /locus_tag="ACHBJ1_002203"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM9431124.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACHBJ1_002204"
     CDS             complement(9444..9830)
                     /locus_tag="ACHBJ1_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9431125.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACHBJ1_002205"
     CDS             complement(10098..10247)
                     /locus_tag="ACHBJ1_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431126.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="ACHBJ1_002206"
     CDS             10709..10921
                     /locus_tag="ACHBJ1_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431127.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="ACHBJ1_002207"
     CDS             complement(11199..12998)
                     /locus_tag="ACHBJ1_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM9431128.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4290 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 ttta
//
LOCUS       DAYYNQ010000083        13058 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000083 DAYYNQ010000000
VERSION     DAYYNQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.083"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..1082
                     /locus_tag="ACHBJ1_002208"
     CDS             159..1082
                     /locus_tag="ACHBJ1_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM9431129.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1088..2341
                     /locus_tag="ACHBJ1_002209"
     CDS             1088..2341
                     /locus_tag="ACHBJ1_002209"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM9431130.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2475..3821
                     /gene="gor"
                     /locus_tag="ACHBJ1_002210"
     CDS             2475..3821
                     /gene="gor"
                     /locus_tag="ACHBJ1_002210"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM9431131.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4635..5588)
                     /gene="menA"
                     /locus_tag="ACHBJ1_002211"
     CDS             complement(4635..5588)
                     /gene="menA"
                     /locus_tag="ACHBJ1_002211"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM9431132.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5601..6704)
                     /locus_tag="ACHBJ1_002212"
     CDS             complement(5601..6704)
                     /locus_tag="ACHBJ1_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM9431133.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6794..7729)
                     /gene="pplA"
                     /locus_tag="ACHBJ1_002213"
     CDS             complement(6794..7729)
                     /gene="pplA"
                     /locus_tag="ACHBJ1_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM9431134.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8031..9965
                     /locus_tag="ACHBJ1_002214"
     CDS             8031..9965
                     /locus_tag="ACHBJ1_002214"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9431135.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10422..10838
                     /locus_tag="ACHBJ1_002215"
     CDS             10422..10838
                     /locus_tag="ACHBJ1_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM9431136.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10852..11418
                     /locus_tag="ACHBJ1_002216"
     CDS             10852..11418
                     /locus_tag="ACHBJ1_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM9431137.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11441..12427
                     /locus_tag="ACHBJ1_002217"
     CDS             11441..12427
                     /locus_tag="ACHBJ1_002217"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM9431138.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12582..>13058)
                     /gene="scm"
                     /locus_tag="ACHBJ1_002218"
     CDS             complement(12582..>13058)
                     /gene="scm"
                     /locus_tag="ACHBJ1_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM9431139.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4066 a         2369 c         2696 g         3927 t
ORIGIN      
        1 atttttgtca caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt
       61 ttgtgaaata attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga
      121 gctatgctac tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag
      181 tagaaactat tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg
      241 gtgaagagca atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat
      301 tggtagaatt agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc
      361 atcctgaaaa aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag
      421 atgtgatcac agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc
      481 gtataccgta tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct
      541 gggcgaaaaa aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga
      601 aggcgatgga agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca
      661 tccatcaatc caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac
      721 aaaaagatcg tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt
      781 ctatttatga agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga
      841 atgaattgaa tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta
      901 ctccaggcga aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt
      961 tagaaggtag ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc
     1021 atcctgaaaa ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat
     1081 aaggagtatg atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc
     1141 gccagcagga acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata
     1201 tattggcggg aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat
     1261 ggctgaagga gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat
     1321 ggtcacccat gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt
     1381 cggtatttct gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc
     1441 cccaggtctg ccgatccatt tatccactca agcttcagca actaattttg agacattgga
     1501 attctggaaa gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga
     1561 agtggcagag attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat
     1621 gtgtatctct tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa
     1681 ccgtggcgga tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg
     1741 tcaagaacga aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc
     1801 agtggatatg tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt
     1861 caagatcgaa ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa
     1921 agcggctgta gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat
     1981 cgacgagtta tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc
     2041 aactgaaaac gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg
     2101 tgaagtatta tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt
     2161 ttctgtagga gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt
     2221 tgaagttatg cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat
     2281 tttaactatg ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata
     2341 aggaaaaagc tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg
     2401 ttacacttgt tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa
     2461 taaggaggaa caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta
     2521 ttgcttcagc aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg
     2581 aattgggcgg aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa
     2641 gtacgatttt ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa
     2701 aaaatgttga tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg
     2761 gtgcttatca aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt
     2821 ttatcgatag tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga
     2881 tcgctactgg aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt
     2941 caaatggatt ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat
     3001 acattgcggc tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat
     3061 atcgaaaaga acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa
     3121 tgtaccaaga agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa
     3181 acaatgacga atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac
     3241 tttttgccgg tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg
     3301 aattggacaa aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata
     3361 tttttgctgt tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg
     3421 gtagacgttt atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac
     3481 ttgttccgac agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag
     3541 aagcattgga aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa
     3601 tgtactttgc attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa
     3661 aagaagaaag aatcgtcggc ttacatgcta ttggtattgg tgtcgatgag atgctgcaag
     3721 gttttgccgt ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca
     3781 tccatccaac aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca
     3841 acagcttttt attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt
     3901 ttttgttttt tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag
     3961 ggaagttgga gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta
     4021 tgcactctta agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag
     4081 aacagatgtt taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg
     4141 ttaaaagaat accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga
     4201 atcaaatcaa gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc
     4261 cgaacaaata gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg
     4321 aggaactatt tttccatttg tttctctaat actcaggaca aaacactgat tttacaacct
     4381 ctgataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
     4441 ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc
     4501 tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     4561 tataaggagc tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca
     4621 acctcttcat gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa
     4681 ctggcattga ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac
     4741 gtttgcttga attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag
     4801 atcggccatt catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac
     4861 atcaatagct ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag
     4921 cgatgatttt caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca
     4981 atcgtaaaca ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct
     5041 accaaagcaa attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat
     5101 gtattgacat aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt
     5161 tcccctaaag gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa
     5221 caacagataa atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg
     5281 ataacaaaaa caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga
     5341 tttctgcggc caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa
     5401 tccatgtaat tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat
     5461 aaaaaagtgt ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc
     5521 gcaaaaggaa atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt
     5581 agtgtcatgg ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag
     5641 tcctccgata atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt
     5701 gtcacgaaga tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt
     5761 ttcacaccca ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt
     5821 atgatcgtga cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa
     5881 tgatggtatt ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat
     5941 gttttgttcc gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg
     6001 atgccgactg tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta
     6061 ccgcccaagt caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg
     6121 tccgtaatgt aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga
     6181 taaacagttt tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc
     6241 tgatcgattt cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt
     6301 gtgattgcac caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc
     6361 cgcttgacta acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca
     6421 ttcacttcat cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt
     6481 actcgatcga aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga
     6541 acgtaagtcc ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct
     6601 gctgaacttt cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa
     6661 gagatgccca gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca
     6721 ttttgaaagg tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag
     6781 tatgattatg tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc
     6841 ctttttcagc tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg
     6901 tagcacctgt aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga
     6961 cgaattcgtc gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca
     7021 tattttcatt atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa
     7081 agtttgattc agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt
     7141 tagagtagtt cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga
     7201 tttcgattgt atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt
     7261 ggaagctttc agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg
     7321 cttttaagaa ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg
     7381 cttccatgtt tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt
     7441 tatcatattt tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt
     7501 aaccatttga ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag
     7561 ctacaacttt tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg
     7621 attcagaaga agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag
     7681 ctgaaaatgc taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc
     7741 accattccat ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt
     7801 agcctataag tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc
     7861 agcaaatttc gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag
     7921 acaaaagaac agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa
     7981 gaattatcat atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc
     8041 aaaatattgt cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa
     8101 agaaactgaa aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta
     8161 tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg
     8221 atctgcagca tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg
     8281 gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc
     8341 tgatccttgg aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg
     8401 gattcaccct ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag
     8461 tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg
     8521 tctgtggttc tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc
     8581 gcctagccaa agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa
     8641 tgccgacgat cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc
     8701 gtaagcacaa cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata
     8761 tcaaattgaa agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca
     8821 aaggaacttc tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg
     8881 ccaatgaata tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt
     8941 tagtctttta tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac
     9001 aaacaggaca cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata
     9061 aatttaaagg aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta
     9121 acctatttga taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga
     9181 atttgaaata cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg
     9241 agtttttcca tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca
     9301 atgtcttatg gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt
     9361 ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat
     9421 catggttcac tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg
     9481 ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg
     9541 ccggagaagc agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac
     9601 caatggctgt gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc
     9661 caaacaatga tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg
     9721 cattagcatt gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg
     9781 tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg
     9841 ctttcgcatt gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat
     9901 ggctacaacg tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga
     9961 aataaataga taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa
    10021 accgtcacag cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc
    10081 atgagcaaga cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca
    10141 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt
    10201 aaattccttc ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt
    10261 gttccacctg ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt
    10321 tctattcttc gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat
    10381 gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg
    10441 ctgaaaaaag tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt
    10501 cttttttacc agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag
    10561 cggtactgcg tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga
    10621 gcaacacata tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg
    10681 atcgagtacg gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg
    10741 caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt
    10801 caacagatgg aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa
    10861 ttacaaaaaa tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg
    10921 gaaaatatga tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat
    10981 ctaatcacga tcgttgccat ttttaccatg aaaaaacgag acagcttctt actactttgg
    11041 ctacgactat tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg
    11101 agtggtgctt tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt
    11161 gtcagtatca tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg
    11221 atggcttctt tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt
    11281 ttagggattt tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg
    11341 cagacacttc aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca
    11401 atgtttacca aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg
    11461 ggaaaactat cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac
    11521 tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg
    11581 aaagctgttg cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg
    11641 agacaaagcc atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca
    11701 tgatgatatc gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt
    11761 tggtaatgtc gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt
    11821 gtctgattac tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt
    11881 attgaatggc gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga
    11941 atatttggaa aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg
    12001 cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc
    12061 tatcgggatg gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga
    12121 aatcggcaaa cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta
    12181 ctctttgcaa aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac
    12241 agaagaagat gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc
    12301 taggaagtta gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc
    12361 cacctctctt cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa
    12421 agactgaata gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt
    12481 ttttgacaaa ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac
    12541 gtccgttagc gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta
    12601 taactcataa aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc
    12661 caattattcg attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca
    12721 ttgcttgttt tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag
    12781 gagacacttg catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa
    12841 gttgatgaat ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct
    12901 gacgtgtccg tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag
    12961 gtagtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat
    13021 gtatccgtac tgtcgcttga agctgacgtg tccgtgct
//
LOCUS       DAYYNQ010000084        12738 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000084 DAYYNQ010000000
VERSION     DAYYNQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.084"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..540
                     /locus_tag="ACHBJ1_002219"
     CDS             97..540
                     /locus_tag="ACHBJ1_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM9431140.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="ACHBJ1_002220"
     CDS             complement(546..1730)
                     /locus_tag="ACHBJ1_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM9431141.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="ACHBJ1_002221"
     CDS             1998..2696
                     /locus_tag="ACHBJ1_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM9431142.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="ACHBJ1_002222"
                     /pseudo
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="ACHBJ1_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3506..4039)
                     /locus_tag="ACHBJ1_002223"
     CDS             complement(3506..4039)
                     /locus_tag="ACHBJ1_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431143.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="ACHBJ1_002224"
     CDS             complement(4336..6717)
                     /locus_tag="ACHBJ1_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM9431144.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHBJ1_002225"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="ACHBJ1_002225"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM9431145.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="ACHBJ1_002226"
     CDS             7418..7957
                     /locus_tag="ACHBJ1_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM9431146.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHBJ1_002227"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="ACHBJ1_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM9431147.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="ACHBJ1_002228"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHBJ1_002228"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="ACHBJ1_002229"
     CDS             9064..10230
                     /locus_tag="ACHBJ1_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM9431148.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="ACHBJ1_002230"
     CDS             10276..11772
                     /locus_tag="ACHBJ1_002230"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM9431149.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="ACHBJ1_002231"
     CDS             11853..12536
                     /locus_tag="ACHBJ1_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9431150.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12545..>12738
                     /locus_tag="ACHBJ1_002232"
     CDS             12545..>12738
                     /locus_tag="ACHBJ1_002232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431151.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2230 c         2630 g         3606 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggttga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DAYYNQ010000085        12734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000085 DAYYNQ010000000
VERSION     DAYYNQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.085"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBJ1_002233"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="ACHBJ1_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9431152.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="ACHBJ1_002234"
     CDS             complement(324..893)
                     /locus_tag="ACHBJ1_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM9431153.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="ACHBJ1_002235"
     CDS             1079..1981
                     /locus_tag="ACHBJ1_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM9431154.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBJ1_002236"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="ACHBJ1_002236"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM9431155.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBJ1_002237"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="ACHBJ1_002237"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM9431156.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBJ1_002238"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="ACHBJ1_002238"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM9431157.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="ACHBJ1_002239"
     CDS             8549..8734
                     /locus_tag="ACHBJ1_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM9431158.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLSTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="ACHBJ1_002240"
     CDS             complement(8796..9992)
                     /locus_tag="ACHBJ1_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM9431159.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="ACHBJ1_002241"
     CDS             complement(10162..11598)
                     /locus_tag="ACHBJ1_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM9431160.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="ACHBJ1_002242"
     CDS             11891..12700
                     /locus_tag="ACHBJ1_002242"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431161.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2672 c         2250 g         4011 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgtca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DAYYNQ010000086        12627 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000086 DAYYNQ010000000
VERSION     DAYYNQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.086"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1933
                     /locus_tag="ACHBJ1_002243"
     CDS             50..1933
                     /locus_tag="ACHBJ1_002243"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM9431162.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2008..2292)
                     /locus_tag="ACHBJ1_002244"
     CDS             complement(2008..2292)
                     /locus_tag="ACHBJ1_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9431163.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(2282..3016)
                     /locus_tag="ACHBJ1_002245"
     CDS             complement(2282..3016)
                     /locus_tag="ACHBJ1_002245"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM9431164.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            3145..3768
                     /locus_tag="ACHBJ1_002246"
     CDS             3145..3768
                     /locus_tag="ACHBJ1_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM9431165.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            3960..5081
                     /locus_tag="ACHBJ1_002247"
     CDS             3960..5081
                     /locus_tag="ACHBJ1_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9431166.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            5088..8216
                     /locus_tag="ACHBJ1_002248"
     CDS             5088..8216
                     /locus_tag="ACHBJ1_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431167.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            8235..11429
                     /locus_tag="ACHBJ1_002249"
     CDS             8235..11429
                     /locus_tag="ACHBJ1_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM9431168.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(11575..12282)
                     /locus_tag="ACHBJ1_002250"
     CDS             complement(11575..12282)
                     /locus_tag="ACHBJ1_002250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9431169.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         4302 a         2218 c         2651 g         3456 t
ORIGIN      
        1 tacccgtata ttacgaatga aaaaagttta tgaaaagttg gaatattgaa tggaaaaaca
       61 aaaaaggtta aaaaacagcc gctacatcac tgggtttgat gggctccgtt cattagctgt
      121 ggtgggtgtt atcttctatc accttctgcc aactagtatg aaaggcggct atttgggcgt
      181 tccgattttt ttcgtcgtat ctggttattt gatcacggat ctgctgcggc aggaatggga
      241 acaaaatgga aaaatcaata tttggcaatt ttatgtcaga agaatgaaga gactctatcc
      301 ggcacttgtt tttatgcttg tgacagcttc ggcttatatc actttgtttc agcgagggct
      361 gttaaacaat cttcgaggag ttgtggtcag cagcttactg tatgtcaaca actggtggca
      421 aatcaataac ggtttatctt atttcgatcg atttgctaat gagtcaccgt ttacgcatat
      481 ctggtcttta gcagtagaag gtcaaaacta tttggtttgg ccgatcttgt ttgtattgtt
      541 gatggtattt gttcgtaaaa gaaaatggat cttttatact gtgttaggta catccttcct
      601 ctccgccatc ttgatgatga tcttgtatac accaggaggc gatccaaccc gtgtttatta
      661 cggtaccgac actcgtctgt tttctatatg gttgggaagt gcgcttgcgt ttgtttggcc
      721 aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa aatattctga atatcgctgg
      781 aggaatctcg cttttggcac tgattctttg cttcttcttt ttagatgatc attacaattt
      841 catctattac ggaggaatgc tcctagtgag tctgttatgc gtagtgctag ttgcagtgac
      901 agcacacccc ggagcaagtt tgaatcgatg gctgacaaat ccagtattta cttggattgg
      961 caaacgaagc tatggcattt atctgtatca gtttccagtt atgatttttt atgaagcaaa
     1021 ggtgactgat attgccgatc atgttttgtt gcatacttta gtggaaatca cattgattct
     1081 aatcatcagt gaactttctt atcgatatgt ggagcgtccg ctagcacgtt ttgattatgg
     1141 tcgtacattc caagtgatga aaggatggtt cagcaaaccg atcatcagta agagcaaacc
     1201 atgggttgtt ccaggcacat tacttgtagc aattgcactc gttggtttcg ttacggcacc
     1261 gaaaaacgct gtaacagccg atcaaaaaca actgcaggca aaaatcgctg aaagtaaaaa
     1321 attagcagaa gaaaccagaa acaattcaga aaacaccact agttcggttg accaagcgat
     1381 ccttgataag tacgagttgt ccaaggcaca agggaataag gcacaaaaat tagaactaac
     1441 ggcatttggt gattcagtta tgttggatgc tgcaacggat ctacaagaac tttatccaaa
     1501 agtagtggta gatggagatg tcggacgaca attatatgcc agtgtaccgt acattgaaaa
     1561 attaaaggaa caagatttgt tgaaagacac tgttctgatc ggactaggaa cgaatggctc
     1621 attctcggaa gctcagtttg atagtgtgat ggatgcaatc ggtgatcgaa aagtatactg
     1681 ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac gtgaatgcga tgcttgaaca
     1741 gatgacgaaa aaatacgaca atatgacgct gattgattgg tacagctaca gtaatgacca
     1801 tagtgactgg ttttatgatg atcaagtcca tccaaatcct gaagggatga cacattacat
     1861 tcacttagtc agcgaagcct tgctaggagg aagttcaagt acgaacagca gtgagacaaa
     1921 cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac atttgttttt gcctgaagtt
     1981 tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg
     2041 tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt
     2101 tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt
     2161 ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag
     2221 atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg
     2281 ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag
     2341 tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta
     2401 acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa
     2461 ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag
     2521 taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg
     2581 tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca
     2641 gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag
     2701 ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg
     2761 cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg
     2821 cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa
     2881 tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag
     2941 aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt
     3001 cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta
     3061 tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac
     3121 cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta
     3181 ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat
     3241 gagaattaca tactagttgc tccccatcgt acttggtggg acccattata tctggcggta
     3301 gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt
     3361 ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca
     3421 agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt
     3481 cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg
     3541 tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc
     3601 aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa
     3661 acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg
     3721 gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt
     3781 atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac
     3841 gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg
     3901 tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa
     3961 tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga
     4021 ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag
     4081 aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag
     4141 aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta
     4201 tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt
     4261 cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg
     4321 tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg
     4381 acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat
     4441 ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat
     4501 ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta
     4561 caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata
     4621 atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag
     4681 gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc
     4741 tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc
     4801 agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga
     4861 atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg
     4921 gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat
     4981 tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat
     5041 ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa
     5101 aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga
     5161 cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg
     5221 atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga
     5281 tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc
     5341 agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa
     5401 acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag
     5461 aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc
     5521 tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt
     5581 tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt
     5641 tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc
     5701 atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac
     5761 cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa
     5821 aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag
     5881 aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa
     5941 taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga
     6001 ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa
     6061 aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga
     6121 ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa
     6181 aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat
     6241 ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat
     6301 tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc
     6361 aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa
     6421 aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc
     6481 tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc
     6541 tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa
     6601 ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg
     6661 aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag
     6721 aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa
     6781 gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg
     6841 caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat
     6901 ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag
     6961 atagacgaaa aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag
     7021 cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc
     7081 agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata
     7141 gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc
     7201 aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga
     7261 cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac
     7321 aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct
     7381 gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc
     7441 atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg
     7501 agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg
     7561 aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct
     7621 acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat
     7681 tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt
     7741 atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt
     7801 taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa
     7861 ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc
     7921 tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga
     7981 tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt
     8041 cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag
     8101 gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa
     8161 taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag
     8221 ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat
     8281 acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg
     8341 cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag
     8401 attactgcca atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg
     8461 aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag
     8521 aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa
     8581 cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg
     8641 agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat
     8701 tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca
     8761 cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt
     8821 ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat
     8881 tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc
     8941 cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc
     9001 agataaatga acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat
     9061 tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt
     9121 tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc
     9181 aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag
     9241 tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga
     9301 tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat
     9361 ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg
     9421 gcgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg
     9481 tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc
     9541 agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt
     9601 tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc
     9661 gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt
     9721 catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc
     9781 tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt
     9841 ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac
     9901 gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat
     9961 tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc
    10021 atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc
    10081 aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag
    10141 ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag
    10201 aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc
    10261 aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa
    10321 aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct
    10381 taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg
    10441 cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac
    10501 ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat
    10561 tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg
    10621 cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt
    10681 tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa
    10741 aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg
    10801 aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt
    10861 gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag
    10921 taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat
    10981 ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt
    11041 atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg
    11101 gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg
    11161 gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg
    11221 ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg
    11281 aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa
    11341 aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga
    11401 ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa
    11461 gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga
    11521 ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac
    11581 aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat
    11641 tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact
    11701 ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag
    11761 catatgaaat tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt
    11821 tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag
    11881 tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc
    11941 cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc
    12001 ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc
    12061 ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat
    12121 tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc
    12181 tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc
    12241 atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg
    12301 gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa
    12361 gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta
    12421 tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta
    12481 atcattatgg tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag
    12541 atttattttt ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat
    12601 ttatttgttt atagtaaatt ttattta
//
LOCUS       DAYYNQ010000087        12527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000087 DAYYNQ010000000
VERSION     DAYYNQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.087"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1042
                     /locus_tag="ACHBJ1_002251"
     CDS             <1..1042
                     /locus_tag="ACHBJ1_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM9431170.1"
                     /translation="FSPILKMLSAIFLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFL
                     LTFLVVIILVVLTVMNVVPQLMEQTIQLTQSLPMYADETSKWLNELAQREEFKNFNLE
                     EQLASANLSLSNLFNIILVSLSASVSKIISFMMQFFILLFTVPFILLFMFKDGHKFID
                     ALSHFFPKAIRKELRQTVKELNETLSAYISSTILDALIIGVMSFLAMRIFDQPYSLLL
                     AVICGITNIIPYVGPFIGAVPAIIVGLFISPFQALYMALSILVIQQLDGNLIKPLLFG
                     KSMNIHPLTIILVLIGAGSVAGILGMLICIPVYAVIKTIILNIRKIYLLRRMEHVSEK
                     LDSPKVLEETSE"
     gene            complement(1131..2096)
                     /gene="nrdF"
                     /locus_tag="ACHBJ1_002252"
     CDS             complement(1131..2096)
                     /gene="nrdF"
                     /locus_tag="ACHBJ1_002252"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM9431171.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(2146..4305)
                     /gene="nrdE"
                     /locus_tag="ACHBJ1_002253"
     CDS             complement(2146..4305)
                     /gene="nrdE"
                     /locus_tag="ACHBJ1_002253"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM9431172.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            complement(4464..4688)
                     /gene="nrdH"
                     /locus_tag="ACHBJ1_002254"
     CDS             complement(4464..4688)
                     /gene="nrdH"
                     /locus_tag="ACHBJ1_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM9431173.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            5089..5562
                     /locus_tag="ACHBJ1_002255"
     CDS             5089..5562
                     /locus_tag="ACHBJ1_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM9431174.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            5559..7691
                     /gene="feoB"
                     /locus_tag="ACHBJ1_002256"
     CDS             5559..7691
                     /gene="feoB"
                     /locus_tag="ACHBJ1_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM9431175.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            7693..7827
                     /locus_tag="ACHBJ1_002257"
     CDS             7693..7827
                     /locus_tag="ACHBJ1_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM9431176.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            7921..8565
                     /locus_tag="ACHBJ1_002258"
     CDS             7921..8565
                     /locus_tag="ACHBJ1_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9431177.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            8752..9945
                     /locus_tag="ACHBJ1_002259"
     CDS             8752..9945
                     /locus_tag="ACHBJ1_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM9431178.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            9938..11665
                     /locus_tag="ACHBJ1_002260"
     CDS             9938..11665
                     /locus_tag="ACHBJ1_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM9431179.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            12059..12256
                     /locus_tag="ACHBJ1_002261"
     CDS             12059..12256
                     /locus_tag="ACHBJ1_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9431180.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            12258..>12527
                     /locus_tag="ACHBJ1_002262"
     CDS             12258..>12527
                     /locus_tag="ACHBJ1_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9431181.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGIN"
BASE COUNT         3944 a         2162 c         2578 g         3843 t
ORIGIN      
        1 ttttagtcca atattgaaaa tgctttcggc aattttttta ccattgcttg ttgctggttt
       61 tatctattat atgttcgatc caatcgttgt ttttctcgaa aagcatcgga tcccgagagt
      121 gttcggtttt ttattgacgt ttttggtcgt aatcatttta gttgttttaa ctgtcatgaa
      181 tgtagtgcct cagttaatgg aacagacaat tcaattgacc cagagcttgc caatgtatgc
      241 agatgaaaca agcaaatggt taaatgaatt ggcgcagcgg gaagagttta aaaatttcaa
      301 tttggaagaa caattagcat cagcaaattt atctcttagt aatttgttca atatcatatt
      361 agtcagttta tctgcaagtg tctcaaaaat catttcattt atgatgcaat tttttatttt
      421 gttatttacc gttccattta ttttgctatt tatgttcaag gatgggcaca aatttattga
      481 tgcgttgtct cacttctttc cgaaagcgat tcgtaaagag ttacgccaaa cggtaaaaga
      541 attaaacgaa acattatctg cctatatcag cagtacgatc ttagatgctt tgattattgg
      601 tgtaatgagc tttttggcaa tgagaatatt cgatcagcct tatagcttgt tgcttgcggt
      661 catttgcgga atcacgaata ttatcccgta tgtcggccct tttattggag cggtaccagc
      721 aatcattgtc ggtttgttta tctctccatt tcaagctttg tatatggcgt tgtcgatttt
      781 agtgatccaa caattagatg gaaatttgat caagccgttg ttatttggga aatcaatgaa
      841 tatccatcct ttgacgatca tcttggtatt gattggagca ggaagtgtcg caggaatatt
      901 aggcatgctg atttgcatac ctgtctatgc agtgattaag acgataatat taaatatccg
      961 gaaaatctat ttattgagaa gaatggaaca tgtttcggaa aagttagaca gtccaaaagt
     1021 gctggaagaa acaagtgaat aagcataaaa aagagttcag tgtacattta aaatatctgt
     1081 acgctaaact ctttttattt ttcgaaaaat ttttgaatta aaataaatcc ttattccaat
     1141 ccaatcaaat aatcgtcgtc tttcatcgct tctacatgac ctaacaaata gccgtttccg
     1201 acttgtgaga agaagtcatg gttgcttgtg cctgttgaaa tcccgttcat tacgattggg
     1261 ttgacatcat tggcagtatc tggaaagagt ggatccatcc ctaggttcat caatgctttg
     1321 ttggcattat agcgtaggaa tgtcttgact tcttctgtcc agccgattgg atcgtataat
     1381 tcttctgtat aacgttcttc gttttcgtaa agctcataaa gaagattgta catccagtct
     1441 tttaacgttt cttgttctgc ttcaggtaac tcgttgaatc ctaattggaa tttgtaaccg
     1501 atgtaggttc catgtacaga ttcatctcgg atgattagtt tgatgatctc tgctacgtta
     1561 gccaatttgt tgtttcctaa ataataaaga ggagtataaa aacctgaata gaacaagaat
     1621 gtttctaaaa agacgctggc aattttcttt tctagcggtg taccgtgttt gtagatttca
     1681 ttgatacgct cagctttttt ttgcagataa ggattcgtat ttgtccagtc aaaaatttct
     1741 tcgatttctt ttttcgtatt cagtgtacta aaaatagaag aataactttt ggcatgaacg
     1801 gattccatga attgaatatt gttcaaaacg gcttcttcat ggggagtgcg gacatcttta
     1861 cgcaactgat ccatgccact ttcagattgg acagtatcta gcagtgtcaa accgccaaat
     1921 acatgtccta ctgtatcttt ttccaattga gacaatgtac gccaatcatc taggtcattt
     1981 gaaagtggga tacgtgtatc tagccaaaat tgctctgtca gcttctccca agtggattta
     2041 tcaatgatat cttcaatttc attccagttt attgcttcat aatatgtttc tgacatgaga
     2101 atgctccttt aattaaaata tgaataagcg aatctatcta tgatattaga tcacgcagct
     2161 ttcacattga ttgcttccga tttcttctgc atcatcagta aaggtacgta cgtaataaat
     2221 ggatttgatt cctttgtgga aagcgtaatg tcttaaaata ttcaaatcac gtgtcgtttg
     2281 cttcgttgtt gtcttccatt cataaatgcc ttctgggatt tcagaacgca tgaataaagt
     2341 caaactcatg ccttgatcga cgtgttgttg tgccgttgca taaacatcga ttactttacg
     2401 catgtccata tcataagcag aagtgtaata cggaatcgtc tcattggata gataaggcgc
     2461 tgggtaataa attttaccga ttttcttctc ttggcgttct tcgatcatgc gagtgatagg
     2521 atgaatactt gcactcgtat cgttgatata tgaaattgag ccatttggtg ctactgccaa
     2581 gcgattttga tgatacagtc catctttcat tactgcttgt ttcaaagcat tccaatcttc
     2641 agcagttggg ataaaaatgc cttcaaacaa tgctttgact ttttctgatt ttggctggaa
     2701 ttctccagtt acgtattttt cgaaataagt gccgtcagca taagctgatt tttcaaaatt
     2761 atggaaaact tgttttcttt cacgagcgat attgttgctt tcaacaagtg tccaataatt
     2821 caacagcatg aagtaaatat ttgtaaagtc taatgaatcc tctgagccat attccatttg
     2881 ttcttttgca agataagtgt gtaagcccat ggcacctaat ccaatcgtat gattcagctt
     2941 atttccgttt tggatcggtg gtacgacatc gatatcagat gcatctgtaa taaatgtcaa
     3001 tgcacgagtc atcgtacgca cagattgacc aaagttaggg ctttgcatca aattcacgat
     3061 atttgtcgag cctaagttac atgagatatc tgttcctaat acttcatatt cctgtttgcc
     3121 gttaatcaca gaaggcgttt gaacttgtaa aatctctgaa cataaattac tcatgatgat
     3181 cttgccatcg attggattct cgcggttcgc tgtatcaata ttgattacgt aaggatagcc
     3241 agattcttgt tgtaattttg agatttcatt ttcaagatca cgcgctttga ttttttgttt
     3301 gcgaatgttt ggatttgcaa ctagatgatc atactcagct gtaatattta cataagagaa
     3361 tgggacacca tattcttttt cgacactata tggactaaat aaatacatat cttcgttttt
     3421 acgtgtcaat tcataaaatt tatcaggtac gatcacgcca agagaaagag tttttacgcg
     3481 aatcttttca tctgcatttt ctttttttgc tgaaaggaaa gcaataatgt ctggatggaa
     3541 aacgttcaaa taaaccacgc cagctccttg acgctgacct aattgattag aatagctgaa
     3601 actatcttcg aataatttca tgactggaac gacaccactt gcagcaccct catatccttt
     3661 gataggagcg ccggcttcac gaagatttga taaagtaatc cctacgccgc caccaatacg
     3721 ggacaattgc aaagcagagt tgattgagcg cccgatagag ttcatatcgt cagtgacttg
     3781 agtcaagaaa caagacacca attcgccccg acgtttacgt ccagcattca agaaagaagg
     3841 tgtagctggc tgatatcgtt gatggatcat ttcgtctgca agagcaagtg ctaattcttc
     3901 atccccgttc gcaaagtaaa gcgcattgaa ggctactcga tcttcataag tttccagata
     3961 agcggaaccg tcgttggttt tcaacgcata ttgagaataa aatttgtaag cagccataaa
     4021 agatttgaaa gaaaaatgtt gctcgtccaa aaattgatat agcttttcaa taaatgatgg
     4081 tgaatacaat tgaatgaatt ctgcttctat atattcttga tcgatcaagt aattgatttt
     4141 ttccataata gatggaaaag tcatcgtatt tggtttgaca ttttcctcaa aaaaggcaac
     4201 taaagcttct ttatccttat tcaaagggat ttgaccattg actggccgat tgatttcatt
     4261 attcagcttg aaataactga catcttgtaa atttttaaga ctcatacgta cgcccaacct
     4321 tcatttcgta tttgatgatg cttgattgca aatcattgca ttgtttttaa gctttaaaaa
     4381 aggatttgga agtttgcgac aaaaactggt gctgtctcaa gcccttcaaa tccatgtatc
     4441 acgttttgat tcttttagtc atgttagctg gcaagtttac gcaactgatc aggacggaat
     4501 ccgataactg tttctgcatc agaagtaatg acaggaacac tttggaatcc ttgtgctttc
     4561 aaccagtcta ctgcttcagg ctgttcatcg atattgactt cttcaaaagc aatttgattt
     4621 tctgttaaaa accgtttgac catcttacat tgcatacaat tatttttaga aaagattttt
     4681 acgttcatgg tgagtgcctc ctctaacaca ttcttattat ctataataga tagtataaaa
     4741 ctcttagtaa aaaagtcaaa caacaaacac tacatattgt gttgtggaaa ctgtggaata
     4801 ctaagttttg tgtttttgaa aaaagagctg aaaacaagta tcgtgtggga aaatagcaaa
     4861 tttaatagag aaaaaattgt ggaaaactat gaagaaatat ttttttctca tttttatcta
     4921 tcaatactaa agattcgctc ataatatcac aagtataatt gacattctca attaccgaga
     4981 gtattctatg tgagaatgat tctcatttat ttgcgtaaca cgtaagaaaa ttagaacata
     5041 taatgaatga ttttaataca tgatttattt cacaagagag gaatcaatat gcagacctta
     5101 gcgcaggcaa agcagaattt cgtttatcaa gtcgaacgaa tcgaaacaga agaaaaaaca
     5161 aaacgccatt tgcaaaatat cgggatcgtt gccggaagta aaatcgtcgt cgtcaattta
     5221 tcaggcaaca atgccatttt actggtaaaa agtcatcgag tggcagtaag tcgggaaata
     5281 ctaggtcaaa tctttgtcaa taaaaagact gcttcctctg aaagctggac ttctcttgat
     5341 caattaaaag caggagagac tgggatcgtc gtagcaatcc acggacaagg cgcagtgaag
     5401 cgacggttaa tggatatggg attgacgcga aatgtaaaaa tagttatccg aaaactagcc
     5461 cctctgggag atccaatcga actgacagtg cgagggtatg aactgacgtt acgaaagtca
     5521 gaggccgaac taatcttgat acaaagggag gaaaaagaat gaatatcgcg ttagtcggaa
     5581 atccaaacag cgggaaaact agcttgttca atctcttgac tggttcgaat caatatgtag
     5641 ggaattggcc gggcgtaaca gtagaaaaaa aagaaggatc gttgaaaaaa aatccgaaca
     5701 ttcatattca ggacctaccc ggcatctatt ctttgtctcc ttatacacca gaagaagtga
     5761 ttgcaagaga ttatttggtt caggaacagc ctgaactgat catcaacatt attgatgcta
     5821 ccaatttaga acgaaatctt tatttaacta cgcagttatt ggatttagac attcctatga
     5881 tcattggtct gaatatgatg gatgtcatca aaaaagaagg aaaaaaaatc aatctagaaa
     5941 agctttccta tggtcttggt gtaccagttt taccaatcag cgtattgaaa aagcaaggaa
     6001 tcgaaaaact aatcgaaaag attcaggagt ctgcaaaaaa gcggccttct attcctgttc
     6061 cctgtacata tgatccacgt ttagaagcag ctttacatga aattgaagat gtactaggag
     6121 aacaaagcgg caagaaacgc tggtatgcaa tgaaatattt tgaacgagat cagaaggtaa
     6181 gagaggatta cgagatagca gtttctcagc aaaaagagat cgagcaactg atccagttaa
     6241 cagagaaact attggacgac gatagtgaga cgattctggt caatgaacgt tatgaattta
     6301 ttacccaact atgtacactc tgtgttgtgt caaatgattc tttccaatta tcgatgagtg
     6361 acaaaatcga tcagattgca acgaatcgtt ggttagcctt gccgatcttc gcatttgtga
     6421 tgtggctgat ctattatctg gctattcaaa cagtaggaac aatggggaca gattggatca
     6481 atgacacatt gtttggtaca tggttaccag aacatgttgg cagattgtta gctgaatggc
     6541 aagtagcggg atggatgcag gagctgatct tgaacggcat catcgcaggc gtgggagcag
     6601 tgttaggatt tctaccgcaa ttgattatat tgtttttatg cttgagcttt ctggaagact
     6661 gcggatatat ggcgaggatc gcttttgtca tggatcgact tttcaggaaa ttcggtcttt
     6721 caggaaaatc cttcattcct atgctgattg ctacaggatg tggagtgcca ggcgtcatgg
     6781 ccagtcgcac gattgaaaac gagcaagatc gtcgtttgac gatcatggtc acaaccttca
     6841 tgccttgttc tgcaaagttg ccgattattg ccttagtagc aggggccttt tttcctcatc
     6901 aatcttgggt agctccttct gcctattttc ttggtatgac tgcaattatt ttctctggaa
     6961 tcagcttgaa aaaaacacgt ttgtttgctg gagatacagc tccttttatc atggaattgc
     7021 ccgcatatca ttttcctcaa tggtcagcgg tcctaagaca aacctatgac cgcagcaagt
     7081 cttttgttaa aaaagcagga acgattatct ttgtttctag tatcgtgatc tggttcagct
     7141 cttcttataa tttttatgct caacctgttt ctgaagacca aagtatctta gcctttttcg
     7201 ggcgaatcct ggcagttgtt tttcagccgc ttggttgggg aaactggcaa ggaacagtcg
     7261 cagcgattac aggattggtc gctaaagaaa atattattgg cacatttggt attttgtttg
     7321 gtcatgcgga agtatccgaa aatggcagag aaatctggca agtgctacaa cacacatata
     7381 ctcccgcagc agcttattca ttgttagcct tcaatttact ttgtgcgcct tgttttgcgg
     7441 caattggggc tatccatcgg gaaatgaatg ccaagaaatg gacttggatc gccataggct
     7501 atcaatgcgg tttagcttat ttagttagtt ttgtgattta tcagttggga cacttattgg
     7561 agggtggaca ggtagcgtta gggacgtatc ttgctatttt actgatgata ggactcgttt
     7621 atgttttgtt ccgtcagcca aaatccaaga accagtttta tactatattg tcattagaag
     7681 gagaggaata ggatggcgac attcttatta agtgtgttaa tctttggaag tgcaggcgca
     7741 attgtctata ctcgtttaaa atccggtaag aattgtgatg actgtcaaac agcttgccca
     7801 gtaaaaaagg aacagatcaa gcgatgatca gtcgttgcaa gaggctattc tccccttttt
     7861 tgcttatttt gctagagcat agtaagatga gggaaagtaa aaaaagaaag aggaaagaaa
     7921 atgtctggaa agaaaaaaat cgtccgcccg cgctaccagc agattgctgt tgatttagcc
     7981 gaacgtatcg ttgaaaaccg atataaagtg ggtgagaaga tccatgcccg ctcaacactt
     8041 gccagtacgt acaatgcttc tcctgagact acccgcaagg ctatcaatgt gctggtagat
     8101 cttggaatca tggaagtacg gcatggaagt ggggcatttg ttgcttcgaa ggaaaaagca
     8161 aaagatttcc tattgcaata tcaagatgta caatccatcc aagatacaaa agcagaaatc
     8221 atggcaagtg tggagaagca acaagatgag ctgaatcatt tttctcaatt attggatcag
     8281 ctggtcaatc aaacgaagcg aatacataag atgaatcctt tacttccgtt tgaattatct
     8341 ttatcagaaa aagcaatgca tctggaccgt tcaattagcg agttgaatat ctggcaggcg
     8401 actggcgcca cagttatcgc aatttctcat aatgaagaac tgcttgtttc acctggcccc
     8461 tatgctaaat ttacggcagg ggacatcgtt cattttattg gaaatgaata tacactacag
     8521 cgtatgacaa acttttttta cccggagcat cagcttgcag aatgattgtg tgaaatatta
     8581 aaaaaatatt aacttggcta aaccttttat ttacaaagat ttagccaagt ttttttcagt
     8641 ttggacaagc aaatttcttt catttttgac aaagtgacta catgatgtta acatagtgtg
     8701 gtcacttttt tcagaaagaa aaatgaattt acataaaaaa ggagaatttg tttggtaaaa
     8761 gtagaagtaa aacatttaac aaagatattt ggtaaaaaga cacaagccgc tttagatatg
     8821 atgaatgatc accagccgaa aacggagatt ttaaagaaaa ccggtgcaac ggttggtgtg
     8881 tatgacgtga attttgacgt aaaagaagga gaaatcttcg tgatcatggg gctttccggg
     8941 agtgggaaat ccacattgat ccgtctattg aatcgtctga ttgagccaac ttcgggcagt
     9001 atctatattg atggagaaga tgtatcgaag ttaagcaaag aagaattgcg agaagtccgt
     9061 cgtcataaaa tcaatatggt tttccaaaac ttcggtctgt ttccacatcg aacgatcttg
     9121 gaaaatacag aatacgggtt agaagtccgt ggtgtaccta aagaagaacg acaagaaaaa
     9181 gcagaaaaag cattagaaaa ttctagtttg ctttcattta aagaccaata tcctagtcag
     9241 ttatctggtg ggatgcaaca acgggttggc ctagccagag ctttagcaaa tgatcctgag
     9301 atcttgctga tggacgaagc gttctcggct cttgatccgc tgatccggcg agaaatgcaa
     9361 gacgaattac tggacctgca agccaatgtt caaaaaacga ttattttcat tacccatgat
     9421 ttgaatgaag ctttacggat tggggacaga atcgccttga tgaaagacgg cgaaatcatg
     9481 cagatcggaa caggtgaaga aatattgacc aatccagcaa atgattatgt acgtgaattt
     9541 gtggaagaag tcgatcgttc caaagtgctt acggctcaaa acatcatggt acctgcattg
     9601 acgacgaata tcgaatctga tggaccaaac gttgcattga caaggatgcg taatgaagaa
     9661 gtcagcatgc tgatggctgt tgacagaaag agacacctga aagggatcat cactgcagat
     9721 caagcgttag aagctaggaa gcaaaaacgt ccattgatcg acttcctaga tgaaaatgtg
     9781 acggtgattg ggaaagacat ggtggtaagt gatattttca atattattta tgactcaccg
     9841 actccacttg cagttgtaga ggatggaaaa ttaaaaggtg tcgtcatccg aggtagtgtg
     9901 attgaagcat tagcagaaac aagcgaggtg agtgaacatg aataatctat tgaactatca
     9961 attaccagtg gcaaactggg tagaaaatat cacagaatgg tttacgacaa ctttttctgg
    10021 cctattttca tttttacaaa cgattggaca ggcagtgatg tcggggatta caaatttact
    10081 gttagtgatt ccagctcctt tgtttatctt gttattgacg gtagcagctt tctttatttc
    10141 taagaaacgt ccaggactta cgttgttcac attaatcggt ctatggttta tttataacca
    10201 aggtctatgg aatgatttga tgaatactgt cacacttgtt cttttatcta gtgtgatctc
    10261 gatcatcatt ggggtaccat tagggatctt gatggcaaaa agcagtaaag cccaaagtat
    10321 tatcaaaccg atccttgact ttatgcagac aatgccagga ttcgtttatt tgattccagc
    10381 agttgcattt ttcggtatcg ggatggttcc aggggtattt gcctcagtca tctttgctct
    10441 gccaccaacc gttcgtttta cgaatttagg gattcgacaa gttcctaaag aattagtaga
    10501 agcttctgat tcattcggga gcacaagtcg tcaaaaacta ttcaaactag aattgccttt
    10561 agctaaaagt acgatcatgg cagggatcaa tcaaacaaca atgctgtcac tttcaatggt
    10621 cgttattgcc tctatgatcg gtgcacccgg cttaggacgc ggcgtattat cagcattgca
    10681 aagagcacaa gtcgggaatg gatttgtcaa cggggttgca ttagttatct tagcaatcat
    10741 cattgaccgt tttacacagc atttgaacca gccaaacaat aaaaaagctg caggagcaac
    10801 aacacagaaa agtaaaaaac gtcaaggact gatcattggt gctgtagtag tagtgatctt
    10861 aggtgcgatt ggtataggga gtttttcatc tgccaaagaa acaaaacgta tcaacttatc
    10921 ttatgttgaa tgggatacag aagttgcttc aacaaatgta gtcggtgaag tattgaaaca
    10981 aatgggctat gatgtaacga tgacgccatt agataattca atcatgtgga aatctgtttc
    11041 aaatggcgag tcagatgcca tggtttcagc ttggctacct aaaactcatg gttcacaata
    11101 tgcacaatat aaagatcaag tcgaagacct aggtgccaat ttgacaggag caaaagttgg
    11161 tttagcagtg ccagcctaca tggatgtaaa ttcgatcgat gagttaaccg atcaggcagg
    11221 taagaaaatc ataggaatcg agccaggagc tggtgtagtt acggctgctg aaaacacgat
    11281 ccaaaaatat gacaacttga aagactggaa agtagagaca tcttcttcag gagcaatgac
    11341 cgttgcacta ggtcaagcga tcaaaaaaca cgaaccaatt gtagttacag gatggacacc
    11401 tcattggatg tttgccaaat atgatctgaa atacttagaa gatcctgaaa acggaatggg
    11461 aagcgaagag cagatccata cgatggttcg aaaaggattg aaagaagacc agccagaagc
    11521 ttacaaagta ttagacaatt tccattggtc agaaaaagat atggaaaaag ttatgttgga
    11581 aatcaacaat ggaaaagatc cacagcaagc agcaaaagac tggatcaaag agaaccaaga
    11641 attagtcgaa agctggaaaa aataaaaaga cagacacgaa ataaatggag cttccaaaat
    11701 tggatcaaat atccaaattt ggaagctcca tgttcgtctc tatctttttt gtctataaaa
    11761 aaagagaatg cgtagctcat cttcgaaacg ttcaaagacg ccgacaaact attatgaatt
    11821 tatgggtgcg agtcaaagag atcgatcgta ggtatgaaag actaatgtga ttaaagttta
    11881 ttatgtacga agtgcattct caaaaaatat taaagaatat acatagttta tatacttaaa
    11941 agtgattaaa ttcttattca ggaattattt tgctacgttt caagaaggaa ttgttttttg
    12001 aaatttaatg aatgttagaa taatagtcta aaaacattaa tttaggggtg attagattat
    12061 gaaacattta aaaattttgt ctattaaaga gacacaactt atctatgggg gtaccactca
    12121 tagtggaaaa tattatggaa atggagtgta ttgcactaaa aataaatgta cggtcgattg
    12181 ggccaaggca actacttgta ttgcaggaat gtctataggt ggttttttag gtggagcaat
    12241 tccagggaag tgctaaaatg aaaaaaaatg ctaagcaaat tgttcatgaa ttatataatg
    12301 atatatctat aagtaaagat cctaaatatt ctgatattct tgaggtttta caaaaggtat
    12361 atttaaaatt agaaaaacaa aaatatgaat tagatcccag tcctttaata aatagattgg
    12421 tgaattatct atattttact gcttatacta ataaaataag attcactgaa tatcaagagg
    12481 aattaataag aaatttgagt gaaattggaa gaactgctgg aataaat
//
LOCUS       DAYYNQ010000088        12426 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000088 DAYYNQ010000000
VERSION     DAYYNQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.088"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            351..1214
                     /locus_tag="ACHBJ1_002263"
     CDS             351..1214
                     /locus_tag="ACHBJ1_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM9431182.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1343..2965)
                     /locus_tag="ACHBJ1_002264"
     CDS             complement(1343..2965)
                     /locus_tag="ACHBJ1_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9431183.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3214..3735
                     /locus_tag="ACHBJ1_002265"
     CDS             3214..3735
                     /locus_tag="ACHBJ1_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9431184.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3921..5651)
                     /gene="spxB"
                     /locus_tag="ACHBJ1_002266"
     CDS             complement(3921..5651)
                     /gene="spxB"
                     /locus_tag="ACHBJ1_002266"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM9431185.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5816..6634
                     /locus_tag="ACHBJ1_002267"
     CDS             5816..6634
                     /locus_tag="ACHBJ1_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM9431186.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6737..6934)
                     /locus_tag="ACHBJ1_002268"
     CDS             complement(6737..6934)
                     /locus_tag="ACHBJ1_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431187.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7103..7681
                     /locus_tag="ACHBJ1_002269"
     CDS             7103..7681
                     /locus_tag="ACHBJ1_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9431188.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7776..7913
                     /locus_tag="ACHBJ1_002270"
     CDS             7776..7913
                     /locus_tag="ACHBJ1_002270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431189.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8726..9229)
                     /locus_tag="ACHBJ1_002271"
     CDS             complement(8726..9229)
                     /locus_tag="ACHBJ1_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM9431190.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9588..10613
                     /locus_tag="ACHBJ1_002272"
     CDS             9588..10613
                     /locus_tag="ACHBJ1_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9431191.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10936..11814
                     /locus_tag="ACHBJ1_002273"
                     /pseudo
     CDS             10936..11814
                     /locus_tag="ACHBJ1_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12290..12406
                     /locus_tag="ACHBJ1_002274"
     CDS             12290..12406
                     /locus_tag="ACHBJ1_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9431192.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         3882 a         2126 c         2403 g         4015 t
ORIGIN      
        1 gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
       61 aactattttt tccgtttttt tctctaatac tcaggtcaaa acgctgattt cacaacctct
      121 gattcacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaatttg
      181 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
      241 cttattttga ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt
      301 tgctataata gacgataagg gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa
      361 ttgtaacaga ttcttgttgt gatctgccat accatgtatt agcaaaagaa aaagttgatt
      421 ttttatcaat gaatctccaa ttgaatgatc aagagttagt agatgacctc ggaaagacat
      481 tcaattatga ccgctacatg aatcagctga aaaatggtgc gatgcctacg acatcacaaa
      541 tcaatgttgg ccgatattta gaatttttcc gttcttatgc ggaaaaacag attcctgttt
      601 tatatgtggc tttttccagt ggattaagcg gctcttacag tagtgcgctt caagcggttg
      661 aattgctgaa ggaagaatat gaagaccctg agatctatgt ttttgataca aaagctgcaa
      721 gtattgggga agggctttta gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca
      781 tcgaagaaac cctagagtgg ctggaaagta attatttgaa agtccattca tgggtaacag
      841 tagacgatct gaaatatcta gaacgaggcg gtaggatttc caaagcaagt gctactcttg
      901 gcagtctatt gagtgtaaag cctattatta cggtggataa agaaggaagg ctgacgaatg
      961 ttgacaaagt tcgaggaaga aacaagtcga tccagacaat cgtcaatgag acggtaaaaa
     1021 caatcgttgc accacttgat caaatcgttt atgtggctta cgctggggat ctagagagcg
     1081 cggagaaggc aaaacggctt ttggaagagc agataaagat gaaagatgtg aagctttatc
     1141 ccttaggacc aacgatcgcc agtcacacag gctatggctg tatcgcgatt ttttcgatgg
     1201 gaatcagtcg ataaaagcag taactcattc tgtaaaaaag cccagaaatt gattgtaaca
     1261 aaaacagcga ctcttaaaag atagatgagc atcctatctc ttagaggtcg ctgttttttt
     1321 attttttaag ctaaataggc cattatgccc ataactcgtt caattgtttt tgaatatctg
     1381 cattttccaa aaattcatca tatgtcgtat cagctcgatc gatcacacct ttatccgaaa
     1441 cggcaatgat gcggtttgcc aatgtttgga taaattgatg gtcatgtgac gcaaataaga
     1501 ttgagccagt aaatgccatc aagccgtcat tcaatgctgt gattgattct aagtctaaat
     1561 ggttcgttgg atcatctaat acaagtacgt tagctttcga aagcatcatt tttgaaagca
     1621 tgcaacggac tttctctcca ccagataaaa cattcactgg tttcaatact tcttcaccag
     1681 aaaacaacat tcttcctaag aagcttctta agaaggtgtt atcatcttct tctttacttg
     1741 catattgacg caaccagtct aaaatcgtta gatcagtatc gaattctttc gttgtatcct
     1801 ttggtaaata agaattactt gttgtcacac cccaacgtac tgttccagta tctggcgtaa
     1861 tttcacccat gataactttg aatagcgttg ttgttgttat atcattttct gcgacgaaag
     1921 cgaccttgtc ttctttgctc aaattaaaag atatatcatc taaaattttc tttccatcaa
     1981 tcgttacact tacattttct acctgcaata agtcgtttcc aatctcacgt tcaggtttga
     2041 atccaacgaa tggataacgt ctagaagaag gttggatatc gtctaaagta attttttcaa
     2101 gcattttttt acgtgaagtt gcttgttttg attttgatgc attcgcacta aagcgggcaa
     2161 taaattcttg gagttctttg atttgttctt cttttttggc atttgcgttt gcttgaagtt
     2221 tagccgctag ttgactagat tccaaccaaa aatcataatt tcctacataa agtttgattt
     2281 taccaaaatc tagatccgcc atatgtgtac atactttgtt caaaaagtga cggtcatggg
     2341 atacaacgat cacagtattt tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt
     2401 ggatatccag accgtttgtt ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg
     2461 tttgtgctaa taacactttt accttttgtc caccagttaa ttcactcatt tgctgatggt
     2521 gcaattcttc agggatgttc agaccttgca gtaaaacagc tgcttctggt tcagcttccc
     2581 atccattaag ctcagcaaat tctccttcta attcagctgc tcgaatacca tcttcgtcag
     2641 tgaaatcctc tttcatgtaa atcgcatctt tttctttcag cacttcatac agacgcttgt
     2701 gtcccatgat gactgtttca agtacagtat agtcttcata gtcgtaatgg ttctgtttca
     2761 atacggccat ccgttcatct ggtcccatag taacggaacc tgtggaaggc tgcagatccc
     2821 ctgataacac tttaaggaac gttgatttac ctgcaccatt tgcaccaatc aagccatagc
     2881 agtttccggg ggtaaattta atattcacat catcaaatag tttgcggtct gaaaagtgca
     2941 aactcacatc atttacagta atcaattttt ttcctcactt ttatcattat tagttttgac
     3001 aagtcttata gaattatata ggggaaaggt aaaagattca aggcaatcga acattctttg
     3061 tgtttcacgt gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa atagctacta
     3121 taggatttat tagtttgcaa cagcataatt tttgacgtta tttataagag ggagtactat
     3181 aaaactgtaa aagaaatgaa agaaggattg aaaatggatg aaaaaagaac catcgactgg
     3241 ggctcattga ttttgggaat tttatttgta cttgtctcat tgttatcatt tcaagaccct
     3301 gtcggaaatt tagttgcaat tgtagtagtc tttgcgattt ttgcctttat taaagggata
     3361 tttgaactgt ttgtacggaa cagattaaaa gagctaacag gatataaagg aaagatgccc
     3421 ttaataatcg ggatcatcga tattctagtt ggcgtctttt tcttattcaa tattggtgca
     3481 ggagtcgcag cattgccttt tgtttttgca gtatggttta ttgctgattc agttttagcg
     3541 ttatttactg ctgacttggc aagaggagta agtgaaggat attattggtt tacgatcatc
     3601 gtcaatattt taggaatttt attaggaatt ttcttattgt ttaatccaat ttcttctgcg
     3661 ttgacattaa gctttttagt tggattctat tttatgttat ttggtatcac acatatcgtc
     3721 tatgcattta gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat tacagtttta
     3781 ctgttgtaat tggaagccgg cttttttatc atggacaaat atgcacgagg tgatttttag
     3841 gtgtcgagac aaatttatgc aaaaatgtga gaagctatat acacaaattt tatcttaata
     3901 ctcgaagcat agcgcagttg tcacaacctc ttttcatata attcattcaa tactggcaaa
     3961 tcatggactt catatttttc tttgaatgct tggatttctt cttcagaata ttttcttgga
     4021 tctaaatgca gatcttctac tggcaatggg cgcttgttat tgatcttcac atcgattact
     4081 acaggacgat ctgattttgc agctgcatca aaggcaaatc gaagttgcga gtattctgtg
     4141 actgtaaagc tatttgcccc gagcgcttct ccaactttac cgaaatctgc atcttctaaa
     4201 aatacaccaa atttcttttg ttccgtatct tcttgttctg cttcgatgaa accaaaagaa
     4261 tcattcgaaa agacaacatt aataattgga agctgatatt tgacttgtgt aatgatatct
     4321 tgcatgacca tagcaaaccc gccatcacca cttaaggtaa atacttgttt ttctgggtag
     4381 cttagttgtg cggcaattcc tcctggcaca ccattaccca tagttgcgaa ccatccagat
     4441 gtcgtatgtt tttgtttacc gttcatattc aataaacgaa tagaatggat agtcgtgtta
     4501 ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac gattgatttc tttatagaca
     4561 gcctctggac gaatcggttc ttcctctcga tcttcaaaag atttcaacca atttacccat
     4621 ttttctttat tttcttgatt tgctaggtac cagctatcag cagggcgagc atcccctaac
     4681 tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg
     4741 cgtcgtccaa attttgacgc atcgatatcg atttggataa attcagcagt tggattaaaa
     4801 aatgcacgtc caaatgggaa gtcgctaccg acaaataaga tcagatcggc ttctgccaat
     4861 gcttcatttc cgggtttagt agcgacacgt ccagcaaaac caagaaagtt ttcataatca
     4921 tcaggaataa ttccttttgc tagaacggaa gcgacaacag gaactgaaaa atgttctgag
     4981 aattctttga tttgcggaaa tccgtttcgc gttccttgac caacataaag aactggtttt
     5041 tttgcttttt caagataagg caaagcagct agcaaatctt tttcttctgg taatatcaca
     5101 cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga
     5161 tcaacaggga tagtcacgat agccacgcct ttatgctcat aggcggcttt aatggcttca
     5221 tctactacat gcggaagact ttgtggtgtc atgactgtac gattgtatac gctaacatct
     5281 gcgaaaatag gattttcatt tagttcctgg aaagagttat agttcatgga tgtagaagca
     5341 acttgtccta agaacgctac tagaggcaca tggtccatct gtgcatcata caaaccattg
     5401 atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag cgccgacttt ccccgttaat
     5461 tttgcatctg cagcggctgc gagtgcgccg acttcttcat gacgaacttg aatgtagttg
     5521 atttcggttt tttcttgata tagcgcatcc ataattgaat tgaaagatcc tccgggaatg
     5581 ccgtaaatat ggtcgatacc ccagctttca agaactttta ccattgcgat tcctgcattg
     5641 atttttgaca tggtgtttca ctcctttagc aatgatgata cataaatttt ttgtatctag
     5701 agtatagaac tttttatcga aaatcccaac taattcgtct tttcacaaaa atgtagtcat
     5761 gtttttggga atatgctaga gtagtacata gatagattca agagagggga atggcatggt
     5821 tgaatggcta ttgagacttg aaccttggca gcaagcactt gctgggacag cttttactta
     5881 ttttatgaca gctcttggtg ccgggttggt ttttttcttt aaagaaatca aaaaagaagt
     5941 gctaaacttg atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt
     6001 gttagatccg gctattacaa aagcagaaga aaatggagat attgcttggc tagttgtaag
     6061 tattgggttt ggtttaggcg gtgtgttttt atatatggca gataaaacac tgccccatat
     6121 gcattttgga ccacaacatg aagcagaagg attgcctacc catttaaaac ggacgatctt
     6181 gcttgttttt tctattacat tgcataacat tcctgaaggt ttagccgtgg gggtagcatt
     6241 cggtgcagca gcaactgcgg ataatccgac agccgctata ttagccgctg tttcggtggc
     6301 gctaggtatt ggtatccaaa acttcccaga aggagcagct gtatcaatcc cgttgcgaca
     6361 agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa tcgttgaacc
     6421 aattgctgga gtgattggag cgctactagt aacgaaagtg gagctgctgc ttccttatgc
     6481 acttgctttt gccgcaggag caatgattta tgtcgtggtg gaagaattga tccctgaagc
     6541 acagcagaca ttgtccagca aacgtcattt tgcagttttt ggcgtcatga gtggatttat
     6601 cattatgatg attttggatg tggcattagg ataaataaaa aaaatatata tgatatatca
     6661 aatagggccg cgaacttata tcgcgaccct atttttttag taagttatat cataatgaag
     6721 tgaatggtat catacattaa taatagtttt ttctatgtgt atttcctttg atccagcgaa
     6781 caagccagat gataagtgcg attgggatta gaactttgac cgcaaaccat aaaagcccgc
     6841 ttaggacact gacaaaaaga ctgatgacga agctagctgc aattagcact aaaagaacca
     6901 tgaagatatt taagaacata tttttttgac gcatacaaaa ccctcctctt gttcattctt
     6961 tactatagaa aaagtataac agggattgtt taaaaacacc tccaactgaa gactgatttt
     7021 tacttcatct acttaaatga tcaaacaatt gctttttctc tggtttatca tctaaaatag
     7081 agcggaaaag gaggagaata tcatgcctaa aaatcgtctt gaagcattta cagatgctgt
     7141 aatcgcgatt atcatgacta ttttagtttt agaacttcat gaacctaacg gagacacgtg
     7201 ggaggcgctc gctgcattag gttaccgatt ttttatctat attgtcagtt ttatcatttt
     7261 agcgatttat tggaacaatc atcatcattt atttcaagtt gtacataaga taaatggacg
     7321 ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc
     7381 atggatcagt gagtacatca atagcctggc tccagaaatg acttatggtt ttgttacctt
     7441 gaccgctaat attgcgtatt atatcttggc aaaagaactt gtcaaagcaa ataaaggcca
     7501 cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat cgattggttt
     7561 gaatgtgtta ggaattgtct tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa
     7621 catcgtgatt ctagctgctt ggatcatccc aagtcgtacc atcgaatcac aatatcggta
     7681 aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa aaataaataa acgatcaaat
     7741 atccgagtga tactagtatt caaagaggga tgcagatgga tcaaaaaaga attcatgtag
     7801 gactgattac tagttttgtc acgatgctta tttatttgga tcaaggtcat ttgacttgga
     7861 cagtagttgg tgtcgtagga tttttgacat tattcacagc aataaaaaaa taaacatctt
     7921 tatgtaatct gatgttctat ctatttaaga aaagcatgtt tacagcagta tgattctgtt
     7981 gtaaacgtgt ttttatttgt ggaaaattta atttttagaa aagtgtatcg aaaaatagat
     8041 agttagtttt tccacagtgg ataacttcgt aagagataaa aataaaaaat tagtcaaaga
     8101 agggcgtata gatttctttt gataggagat atacaacaga aatgaataaa tattaacgtg
     8161 ttttcaaaca gtataaaacg gtgtggtttt tggaataaaa aaataacata tctcatttta
     8221 cacttttgtt attttatact taaaaaataa cgacacagaa aataccgtgt ctcagatttc
     8281 tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa agtaggatca
     8341 ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa aaaccataga
     8401 aaatataaag taattactta taaaacaagc tttttataga aaaataatat acctgagtag
     8461 ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa gaatgttaag
     8521 attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt
     8581 cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga tgaaaataga
     8641 tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg aatgtgtcta
     8701 ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc aaaaataaaa
     8761 aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga cggtctaact
     8821 tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc agtcctcctg
     8881 ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg aattgaatca
     8941 attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa gaaagtctct
     9001 tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta taagtggtct
     9061 cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc
     9121 tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa atcaaccata
     9181 atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata gacatctcct
     9241 ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa
     9301 ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa tccggtttct
     9361 ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc tgtcaaaacg
     9421 actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt gatactaagt
     9481 ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg gattggcatt
     9541 tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg ttcaagactt
     9601 attttcgttt caataaaata caagataatc cttttcgtat catgaatctg atgctactta
     9661 tctttattgt attatggtta ggttacataa atcatgattt tttgatttca agtttttcat
     9721 cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat ttgctgaaac
     9781 gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc ttatcgactt
     9841 atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc agattcgtgt
     9901 tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg
     9961 gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat ttttttatgg
    10021 gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca cggcctgagc
    10081 agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta gtgatcattg
    10141 attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac ggcttgaatc
    10201 tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca gaaaacttgg
    10261 atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg ttatgtgtgt
    10321 cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg atcacttttc
    10381 tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat atttttctta
    10441 atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt tctttgatcg
    10501 aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa
    10561 tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa tagaaggaca
    10621 aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa ttcaaggtgt
    10681 tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa aggaactttc
    10741 aaaaaaattt tatatttatt ctatcaaaaa attttgttta tgatggtaac tgaataaact
    10801 ttcctgagat ggtcaatgct ttccttgaaa gtcttacaga tattctttct tcatagaaag
    10861 tttaagtttt atagttagtg tacaataaaa gtatgtttat accatatggt gtccggataa
    10921 tcagtcattt aaacaatgag gttcaaagat atgaaaaggg gaaatttgat gaagaaaata
    10981 agcaatgaaa tccaaattct ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa
    11041 atgattgtac aagcaaaaaa catccttgat gaggtgtttg agcaatatag tcatgaacta
    11101 atggatccta ataaagcaaa ggcgactgca aagaaaatag acaaagtcta tgaagaggaa
    11161 ccttttgcag cattagctat ttatctatat gcagcttctc aaagttggaa aaaaatttat
    11221 caaccattga agattccaag aactatctat ttggcaacaa tgaatgcttt tactagattt
    11281 attcatgaac atttccaagt aacaggaagc tatcgctttg accgtggttt ttggatttgg
    11341 cattacacta gtggcttgat ctttcggatt ggtgaattgg agtacgaaat gacatatttt
    11401 ccgaaagggt acacggatct aagattagaa ggaaagcagt gcctttctct tcatattcct
    11461 tccgatgcag atttatctca gaataagata agagatagtt acgaaagtgc cattcgtttt
    11521 tttgaacttt attttcctga ttaccattac caagtgatgt acacagatac ttggttacta
    11581 tcacccaatc ttactaaata gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat
    11641 tatcgattat tatcagttga tgaacaggac gatagtggag tcccttggat ctttggacga
    11701 gtagatgccc aaatacatga ttatccagaa agtacgagtt tacagcgaca agcaaaagag
    11761 caattgcttg caggcgagca cattggctct ggcttaggta ttcttacttc ataagtttag
    11821 tacttcgaaa tagtttaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
    11881 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc tgaaattcac
    11941 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
    12001 tcccagcctc ttttttagtg ttctatcgaa cacacagtac gttcaatagg taaactagct
    12061 ataacaatag atattaaagt taacggacaa gagggccttt tttaggtagc taaaacgtaa
    12121 taatgaaaat ttatttccag agataggagt caatcagcta gtctgatcga ctgttttaac
    12181 aagttgactt tcattttaac ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat
    12241 ttgtgttttt tagcatgact acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac
    12301 agatatctca agtgcatacc gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc
    12361 gctgaagatc atcaaagatg tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt
    12421 aattaa
//
LOCUS       DAYYNQ010000089        12397 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000089 DAYYNQ010000000
VERSION     DAYYNQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.089"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..400)
                     /locus_tag="ACHBJ1_002275"
     CDS             complement(38..400)
                     /locus_tag="ACHBJ1_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431193.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(474..854)
                     /locus_tag="ACHBJ1_002276"
     CDS             complement(474..854)
                     /locus_tag="ACHBJ1_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM9431194.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            936..2111
                     /locus_tag="ACHBJ1_002277"
     CDS             936..2111
                     /locus_tag="ACHBJ1_002277"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM9431195.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2188..2772)
                     /locus_tag="ACHBJ1_002278"
     CDS             complement(2188..2772)
                     /locus_tag="ACHBJ1_002278"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9431196.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2973..3971
                     /locus_tag="ACHBJ1_002279"
     CDS             2973..3971
                     /locus_tag="ACHBJ1_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9431197.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4083..4592
                     /locus_tag="ACHBJ1_002280"
     CDS             4083..4592
                     /locus_tag="ACHBJ1_002280"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM9431198.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4622..5281)
                     /locus_tag="ACHBJ1_002281"
     CDS             complement(4622..5281)
                     /locus_tag="ACHBJ1_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM9431199.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5283..6047)
                     /locus_tag="ACHBJ1_002282"
     CDS             complement(5283..6047)
                     /locus_tag="ACHBJ1_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM9431200.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6055..6732)
                     /locus_tag="ACHBJ1_002283"
     CDS             complement(6055..6732)
                     /locus_tag="ACHBJ1_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM9431201.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6769..8154)
                     /locus_tag="ACHBJ1_002284"
     CDS             complement(6769..8154)
                     /locus_tag="ACHBJ1_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM9431202.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8364..8448)
                     /gene="ffs"
                     /locus_tag="ACHBJ1_002285"
     ncRNA           complement(8364..8448)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHBJ1_002285"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8471..8986)
                     /gene="tadA"
                     /locus_tag="ACHBJ1_002286"
     CDS             complement(8471..8986)
                     /gene="tadA"
                     /locus_tag="ACHBJ1_002286"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM9431203.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9092..9949
                     /locus_tag="ACHBJ1_002287"
     CDS             9092..9949
                     /locus_tag="ACHBJ1_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431204.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10030..10365)
                     /locus_tag="ACHBJ1_002288"
     CDS             complement(10030..10365)
                     /locus_tag="ACHBJ1_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431205.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10432..11802)
                     /locus_tag="ACHBJ1_002289"
     CDS             complement(10432..11802)
                     /locus_tag="ACHBJ1_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM9431206.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11880..>12397)
                     /locus_tag="ACHBJ1_002290"
     CDS             complement(11880..>12397)
                     /locus_tag="ACHBJ1_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9431207.1"
                     /translation="YSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYTHFKS
                     ISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDS
                     LFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDE
                     YLENILKEQSP"
BASE COUNT         3673 a         2526 c         2090 g         4108 t
ORIGIN      
        1 aaaaaattca aacttctttg ttcgcttaat ctgtatttta gaagaaaggc actccctcta
       61 cttcgtaagt tccaagaaca gagcgcattt gacctggttc ttcccaaata tatccttcgc
      121 ctaattcttc tctttgattc ttttgttcag tttcaaagac aaacaacgga acattttcct
      181 ctttaatatg tgtatttgtc aattccatca aactaatctt gctaacatcg atatgaacta
      241 tttcttttaa gaaattaagt atattcgcta aaccagtctt tccttcagaa agttccgcta
      301 acgcaaattc caacttgtca tcaattgaat ggaccaaaaa ttttttagta ccatcctcca
      361 aatgcaacat aattgtacca gcaactcttt tgcttgccaa aatttccacc tcgatcaact
      421 tgttctatgg cttacataaa aattctagca cattttcaga aaaaatacac tttttatttc
      481 ttattttctt tccgttctag ttcagataat gcttcttcta tccaaatagg taactgctcc
      541 gcaatagtgg taaatccgat cttacgattt ttattcgtga aatacctttt tctgcgatat
      601 ggaacagctg gatttgtttt ttctaacgtt cttaaagata aactgatttt tttggaatat
      661 tcatcaatgt cgatgatctg aactgttact ggctgtccga cttttaacaa tgaatggata
      721 tttttcgtat aaccggattg tacttctgaa acatgaataa gtccttgcgt ctcgttatca
      781 agagaaacga atgcgccata aggctgtatc cctgtcactt ttccttcaat tatttgtcca
      841 attttataag tcatctactt ccctctcttc gcatttatca ttatactgta aatagctcaa
      901 aaaaagaagc gacgaaaatg actggaggtc ttaagatgat cgaatttgat aaacagattt
      961 caagaaaaga tacagaaagt gtaaaatggg acgcaattgc tgagacatat caaatggatg
     1021 acttacttcc tttgtgggta gcagatatgg acttcttgtc tcccggtggg gtagctaaag
     1081 cgtttagtga atacatcaaa catggaatct ttggttatgc cactctgtct gataaactat
     1141 atcaggctat cattcattgg gaacgccaac accatgctgt ggagttgacg aaagaaaata
     1201 tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc agtacaaact tttacaaaac
     1261 caggcgattc tattttgatt catgacccag tttatcctcc attttcctct attatagaaa
     1321 caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa aaataatcat ttcatcatgg
     1381 acttgtctga catggagaaa aaaatcgaag aaaacaatgt aaaagcaatg attttatgca
     1441 atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga attgaaacag ttaagcgaat
     1501 tgtgtttaaa acatcaagtg attctgttta gtgacgagat ccatcaagat ttaactttat
     1561 tcgatcatac atttacttcc atgctaacga ttgatcctaa tttagatgat ctactagttg
     1621 cttttacttc cgcaaccaag actttcaatc tagctgcaat caaaaattca atggtattta
     1681 tcaaaaatcc agagatgaaa gctgattttg aaaaacattt agtgatgaac caacagcacg
     1741 aaatcaacac ttttggactg attggaaccc aagcggctta tgagacgggt ggagaatggt
     1801 taagccaact gattccttat ctagaaaaaa atgttcaaac agttaatcag ttttttgaac
     1861 agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac ttacttaatg tggttagatt
     1921 ttaccgccta tatttcagac gaccaagaat tggagaacac tcttgttcgg aaaggaaaag
     1981 tcgtattaaa tcctggcatc acgtttggtc cttccggtca tgggcatatg cgcttgaata
     2041 ttgcttgtcc agaagaaact ttactggaag gacttaatcg aatcaagtat gctttagaga
     2101 caatcgatta aatcatttat tcaaaataaa aggacgagac cttactaggt ttcgtccttt
     2161 ttcataatta ctccgtacca taccaaacta ttctgaaatt tcaattgttt caatcactac
     2221 atcatgtaca ggtcggtctt gtggaccacg ttgtacattt gcgatttcat ctactgtgtc
     2281 catcccttct agtacatgtc caaagactgt atgtcggaaa tctagccaag gagttccgcc
     2341 acctttatac gcttcaatga tttctgatgg gaatccagca ccttctagtt gtccaagcat
     2401 attagctggc acatttgtgt tatttacaat aaagaattgg cttccgtttg tgtttggacc
     2461 tgaatttgcc atagataaag ctccgcgaag gttgaacagt tcacgactga attcatcttc
     2521 aaaactttcg ccataaatac tttcgccacc cattcctgtt ccagttggat cgccgccttg
     2581 gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca tcataataac cttttttgga
     2641 aagttcaatg aaattttgaa ccgtcttcgg tgctagttca gggaataatt gaacagtaat
     2701 gtcaccacgg tttgttttga taacagcttt tggtcctttt tcacttttta aatctagttg
     2761 tgggaatgcc atattgtttc ctcctaatat ttgcatactt cataataata ataacataat
     2821 acctaacctc tcgtaaaaat actagatttg ttttctttta tttttcatca ctagatacat
     2881 tctataactt accgtttcga tttcgaattc aaacattttt gagaagcatt ctcaattaaa
     2941 ttctgatata gtgtgaaaag ggaggaattt ttatggaaat ctatgacatt acaatcatcg
     3001 gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg aatgcgccaa gctaaaacaa
     3061 aaatcattga cagtctacct caacttggtg gtcaattagc aactttgtat cccgaaaaat
     3121 atatttacga tattcctggg tatccagcaa taaaagcaag tgaattaatc gatcaattag
     3181 aaaaacaatt aactactttt aaccatacat tccatttaaa agaagaagtt ctctcgctta
     3241 ctcgtgaaga cgaagttatc gagattacta caaataaagg catccattat tccaaagcag
     3301 tcattttagc tttaggaaat ggttcattcc agccaagaaa attgaattta gataatgctg
     3361 aatcatttga aaaccacgga ttggattatt tcgtgaatga tttgatgaag tatgctggaa
     3421 aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga ttggacgttg atgttagagc
     3481 ctattgcaag cgaagtttac ttgattcatc gtcgcccgga atttcgtgcg cacgaacata
     3541 gtgtatcacg cttgaaatct tcatctgtca acttgcttac tccctatctc atcgatggat
     3601 tatcaggcaa taatggggaa ctgacagata tccggttaaa aaaagtgaaa agtgacgaga
     3661 cgattgattt gatgattgat tctctgatcg tcaattatgg attctcttct aatttagaac
     3721 atctttcttc ttggggactt gatagtacca gaaatgccat tactgtaaaa tcagatatgt
     3781 ctacttctat tccaggcgtt tatgcagctg gtgacatctg tacctatgaa ggaaaagtga
     3841 aactgattgc tactgggctc ggtgaagcac ctactgcagt gaacaacgct ttacactata
     3901 ttaatccaaa ggaacgaact cagcctggtc acagcacaag cttatacgat aaaaatcttc
     3961 gtcctagttg attttttata tctctttgca tttcaagtta aacccgaaag cagtttcttg
     4021 catttttttc atgttccgtt aacatgaaat agagaaagaa aaaataaagg gggagcaata
     4081 atatggtcgt caaaactgag actttagaag aaaaggcaag agaactattg aaggaacgtg
     4141 gcgtaactgt agaagatatc gcacaacttg tcatgttttt acaaaaagac tatatttctg
     4201 atttgacttt ggcggattgt actgaaagtg tcaacagcgt gctaacaaag cgtgaagtac
     4261 ataacgccat tatcactgga atccaattag atatattagc agaaaagaaa aaattgatga
     4321 gtccgttgca acatattatc gaagatgacg aaggccttta cggtatcgat gagatcctag
     4381 ccttgtcaat cgtgaatgtt tacggtacga tcggctttac gaattatggt tatatcgaca
     4441 aggtgaaacc tggtatttta aaagaactaa acagccacga tggcgttcat gtccatacct
     4501 tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc cagtcgatta gcacacaaag
     4561 aaccagaaaa aagaagtcgc cttactcaat aattcaacaa aaaaagaaag aaaacgtcta
     4621 tttagacgag ttctttcttt tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa
     4681 cagcaaaaaa taattctaat aatacaaaaa ataagataaa gctatgcatg aaaaattctt
     4741 ctggaagtac attgatgatt ggatcagtaa cgggatcgaa taaccagtca tcgttgttga
     4801 aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga
     4861 aaacaactgg cagtaacatg ccgatttgaa acggacgaat caaccgccaa agccgatcat
     4921 tcttttgcaa atatttgata aacaaaatac tcgggatcaa agtgatgaaa aacacagcat
     4981 aatccactaa aaacaaaatt ttcacttcat aaaagtgatg ggctccgcta gcagaaactg
     5041 ggaaatctgg caagctgagg atggttttga agggattatt gagataattc attaggtgat
     5101 caaaattttt cagtaacgtc tcttgatcta aagaagtata atccaaaatt tgcaaatgtc
     5161 cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac accaactgcc agactgatca
     5221 gtgtcagaaa taggctgaac aatccagcat attccatcca ctgccatctc gaatttttca
     5281 tattagaagc tccattcatc cagtgattgt ttcacatagg tagctgggat aggcaaccca
     5341 ggtacatctt cttcttttgt aaaacctgat aaaacgagca aagtatctat attattgcgt
     5401 atcccagctt ggatatctgt ttcatagtta tctccaacca tgattacttc ttctttttgc
     5461 agccctaata cttcaacggc tttatccata atgatggctt ctggttttcc gatgtagatc
     5521 ggcggcgttt gggttgctgt ctctacaaaa gagatgactg accctgctcc aggaagaagg
     5581 ccgcgttcag taggtatatt cttatctggg ttcgttccaa tgaaagtagc acctttttga
     5641 atagcaagtg ttgctttaac tactttttca taggtcagat aattatctaa gcctaccact
     5701 acatagtcag gagcctcttc ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat
     5761 cctgcttctc cgataacata gacttttttc ccttttccat ctgctttcat aaaatcgatt
     5821 gttgccaaag tagctgtgta gactgtctct ggagctacat gaatgtcaaa ctcatctgct
     5881 agacgattag ctactgtttc tggtgactta gtcgtgttat tggtaacaaa caagaaaggt
     5941 aattttcttt tttgcaattc ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca
     6001 cgataaatcg ttccgtctaa atcaattaaa tatcctttat agtccattca tccactactc
     6061 ttcccttttt ctgatcgtaa atgctgtttt tgttttttct acttgtttag gttgttcttg
     6121 tttttttact ttttcagagg tttttgctga ctctgttttt cgatttttaa taaccggttt
     6181 tttgcgatta gatgcgactg gtcctttttt ctcatcgata tacgcacgat tatggttttt
     6241 tttctttttt cggcgctgag ttgtcttttc ttttttaccg ccgattctct cgatgacaaa
     6301 atacgcacaa ccaaaattgc aatattcgta aagatagtct tccaaggaat cgattcgctg
     6361 atctggatgc gcccgtcgat tatccgcttg gaaaaatcct tttaggcgta gttgttcata
     6421 accccaatct cctacgatat aatcatatcg ggctagtaca tcactgtaac gttctccaag
     6481 cttttctgca ttaaatcctt cacgataatc ggacacaagc cgataatgtc tttcacctat
     6541 caaaaaatta ctgtcatcta acagtgtcac tagctcgcct ttgacttttt ctgctttagg
     6601 ctcctcaacg atttcttcca aagcagctgt cagttcttcg gtgactgttg tttcctgttc
     6661 agctgatgat tttttcttag caatttctgt tttatgctca acgttctgct gttcattttg
     6721 ttttgcagac atgcgttcac cacctatctt ttttattata ccttgttttt atttgattgg
     6781 gtagtgagct ttcaagtaat ctcctaccac acttcggata aattcaggga acaaaaattc
     6841 attttttcca gcaatctcaa tcgttggaaa aaatggaaca aacatcaaat gatctacggt
     6901 agtaaaagta taatttttat tcagatcgat tggcttgttc ttccaaaaga cttgatggtt
     6961 aatagaatca tatgtgattc cattatagac catctgccca aaaatcttac cgcgaaatcc
     7021 cattcccttc ataggatagt tacgtaaaaa gttacgattt ttttctattt ctaaaactaa
     7081 gcggatcaga tcatttccac ccaatgtgac atttaacaag tgcatcggat gcggcaacgc
     7141 agtgtgaagc tgatcctgat tgacccgccc ttttggaatt tcagttaaaa acagaccact
     7201 gtttaatatt gctgcttccg tctttcctcg ttctttcacc gcctcaaagg ctacttggat
     7261 aaacgaatgc tcattgaaga tatctagact cagagaaaat ggcaaatctg ctacttcttt
     7321 ttcttttaat aattcatgcc cacgttctag acaactattt atttcttgtt catcttctgt
     7381 aaaagctgtc attgtttcgg tagggatggc tttggctgaa tgcttaagga tttttttctc
     7441 ttcatcgatg gttaaatgga cttcaccgac atactgcccg tactttccag cggctgctat
     7501 ttggacatca ttgattacgt gaccttctct gaataaatga tgggtgtgcg atcctaaaat
     7561 aacatcaatt gctggtattt cttttgcaat ttggaaatca tcctgaatcc cgagatgact
     7621 catcaaaact aaaacatcca cttgtgaacg taaatcttcg actaattctg gcaaaatatc
     7681 ataaggattc cgtatgtccc agccattcgg attataagta agcggaaatg gagcagtaaa
     7741 tgcaattagt ccgattttcg tctggtgttt cgttgtaatg attttatatg gtttcgtcca
     7801 tgccggtggc tggagcgtat ttttatcgaa taagttatcc aggatgatat caaactcagc
     7861 atgatcgtat aaatgattca actcttcttt ggaatttcct acgccttcat tgtttcctat
     7921 cgtcgctgca tcataaccaa cttcattcat cagcaagata ttttcctgtc cattcgttgc
     7981 ttctgacaat ggatgccaac gatccacaaa atcacctaga tctactgcaa tcgttgtcgt
     8041 tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa aaccttctga tccttggcca
     8101 attttctaaa tgagaatgca gatcattcgt atgtaaaatc actatttttt ccatgtaccc
     8161 tctcgcctct ctttatcttt atcataccat aggcagcttt ttcttccatt gaaacaagct
     8221 ctatttcaga gtttttcctg ccttgaagca aattgtataa gtacaatatt cgataaatca
     8281 gtctggctaa aaaatcagga acattcattt ttacagtact ataaaaataa aaaaaacaga
     8341 cccgaaagtc tgttgtcgtt tcattataat cagacacatg gcggaacctg cttagtgctg
     8401 cttccttcca gacctgacac gcttcacaag cccaccattg tcctagcctt tcggcaaaga
     8461 atagtatacc ttatttttca ttcgttgacc agtctttttt taatttcttt tttcgctggc
     8521 ggagctttcg gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa atgccacctt
     8581 ctacatatgc ttggtggtta aaccgctcgt cctccaaaag attcatcaag gtaccggctg
     8641 ttccgccttt tggatcatac gcgccgaaat aaacttctgc tacacgagac aaaatcatcg
     8701 cgccgctgca cataggacaa ggctctaagg taacaaaaag tgcagcatct tccaagcgcc
     8761 agcttcccac tttttcacag gcttccttga ttgcaagcat ttctgcatgt gtagtagcat
     8821 cttgcgtggt ttctctaagg ttatatcctc ttccaatcac ttttccatcc aatacaacca
     8881 ctgctccaat cggaacttca tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc
     8941 gcatccattt ttctttttct actgcatcaa gatttgtatt taccaattac ccttccctgc
     9001 ctttgttttt tcttgtttta ctttagcata tgataaaaag gctgtaacta actttttttc
     9061 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9121 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9181 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9241 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc actatctttt
     9301 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9361 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9421 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9481 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9541 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9601 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9661 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9721 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
     9781 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
     9841 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
     9901 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
     9961 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10021 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10081 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10141 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10201 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10261 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10321 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10381 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10441 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10501 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10561 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10621 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10681 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10741 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    10801 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    10861 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    10921 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    10981 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11041 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11101 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11161 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11221 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11281 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11341 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11401 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11461 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11521 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11581 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11641 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11701 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    11761 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    11821 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    11881 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    11941 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12001 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12061 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12121 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12181 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12241 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12301 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12361 accatacttt tgaacagcaa atattcttta ctataat
//
LOCUS       DAYYNQ010000090        12135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000090 DAYYNQ010000000
VERSION     DAYYNQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.090"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..610
                     /locus_tag="ACHBJ1_002291"
     CDS             <1..610
                     /locus_tag="ACHBJ1_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9431208.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACHBJ1_002292"
     CDS             723..1001
                     /locus_tag="ACHBJ1_002292"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9431209.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACHBJ1_002293"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACHBJ1_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM9431210.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACHBJ1_002294"
     CDS             1458..2213
                     /locus_tag="ACHBJ1_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9431211.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBJ1_002295"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACHBJ1_002295"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM9431212.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBJ1_002296"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACHBJ1_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM9431213.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBJ1_002297"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACHBJ1_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM9431214.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBJ1_002298"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACHBJ1_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM9431215.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBJ1_002299"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACHBJ1_002299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM9431216.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACHBJ1_002300"
     CDS             6950..7492
                     /locus_tag="ACHBJ1_002300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM9431217.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBJ1_002301"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACHBJ1_002301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM9431218.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACHBJ1_002302"
     CDS             9085..9987
                     /locus_tag="ACHBJ1_002302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM9431219.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBJ1_002303"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACHBJ1_002303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM9431220.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACHBJ1_002304"
     CDS             11430..11852
                     /locus_tag="ACHBJ1_002304"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM9431221.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DAYYNQ010000091        11907 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000091 DAYYNQ010000000
VERSION     DAYYNQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.091"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..465
                     /locus_tag="ACHBJ1_002305"
     CDS             <1..465
                     /locus_tag="ACHBJ1_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFM9431222.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="ACHBJ1_002306"
     CDS             complement(1080..2120)
                     /locus_tag="ACHBJ1_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9431223.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="ACHBJ1_002307"
     CDS             complement(2469..2795)
                     /locus_tag="ACHBJ1_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431224.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3570)
                     /locus_tag="ACHBJ1_002308"
     CDS             complement(2782..3570)
                     /locus_tag="ACHBJ1_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431225.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="ACHBJ1_002309"
     CDS             complement(3938..4228)
                     /locus_tag="ACHBJ1_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9431226.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="ACHBJ1_002310"
     CDS             complement(4364..4543)
                     /locus_tag="ACHBJ1_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431227.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="ACHBJ1_002311"
     CDS             complement(4540..4890)
                     /locus_tag="ACHBJ1_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431228.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="ACHBJ1_002312"
     CDS             complement(4883..6208)
                     /locus_tag="ACHBJ1_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9431229.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="ACHBJ1_002313"
     CDS             6658..7272
                     /locus_tag="ACHBJ1_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431230.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="ACHBJ1_002314"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="ACHBJ1_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="ACHBJ1_002315"
     CDS             complement(7883..9202)
                     /locus_tag="ACHBJ1_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM9431231.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9336..9944)
                     /locus_tag="ACHBJ1_002316"
     CDS             complement(9336..9944)
                     /locus_tag="ACHBJ1_002316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431232.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10581..10838)
                     /locus_tag="ACHBJ1_002317"
     CDS             complement(10581..10838)
                     /locus_tag="ACHBJ1_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9431233.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10831..11100)
                     /locus_tag="ACHBJ1_002318"
     CDS             complement(10831..11100)
                     /locus_tag="ACHBJ1_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9431234.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11231..11410)
                     /locus_tag="ACHBJ1_002319"
     CDS             complement(11231..11410)
                     /locus_tag="ACHBJ1_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431235.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11508..11744
                     /locus_tag="ACHBJ1_002320"
     CDS             11508..11744
                     /locus_tag="ACHBJ1_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431236.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3674 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
    10021 tttttttatt actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt
    10081 ggggagtact tctttcaaat tcaagttaaa tttatattcc attacttctc ctccaatata
    10141 taaaaattac ttaactgtat ccaaatttaa tatgtaaata aatcattaat ttttagctaa
    10201 attctatgag aagtttattt ttgttgtttt tgctaattaa ctaaaactag tacaaaaaat
    10261 ttaaaataga ttattaaacc tattgcttat tctattcagt catatatata agcgtcatcc
    10321 tttagtaact aacatcccac actacaatag tattataaca tatctattca acttcaaatt
    10381 gttgaaattt aagccattta tatttacttt tcctcaactt tagaaatgat tttactaacc
    10441 ttatatccgt aatacgcgta acaaacacat tcattaataa caatcaacac taagctatat
    10501 tagcttttta aatgactagg gtaaataaaa taaggatcaa aaacttttaa gtttctgacc
    10561 ctttccttac ttccgattgg ttaatagtga tcttttgcag aataaataaa tatcgtttca
    10621 ttttcaactc tatatatcag tctatgttca tctgtaattc ttctggacca ttttccagat
    10681 aaatcatgct ttaatggctc aggttttcct aatccagcaa agggggaacg atcgatatct
    10741 tttattaact tgttaatctt ttttatattg cttttgtttc cttgctcatg ccaataaaga
    10801 taatcatccc aagcatcatc agaccaagcc ttaatcatca gattcaacct cgattaagtc
    10861 atgtgtttta aatgcacctt tggagaattg ttcatctcct cgacgaattt tttccatgac
    10921 gtaattatta gaaagtgttc tcaacgtttc ttgcatagaa tcataatctc tttttgataa
    10981 tacaacaact gtatcttcta catctttact tgttacaata agtgtttcag catcctcatt
    11041 aacttgtttc atataactac gtaaattttg gcggaaattt gaataagcta ctgcttccat
    11101 tcctttcacc ccttatttat ttctataaaa caattgtaca ttatattgta caattaagca
    11161 aatgattaat attctaactt cttataatta aaagtgctat cacttttagt ttttaggata
    11221 aaaagtgacg tcactttttg agtgctggta cctcaaatcc tgctttttct tgggcaatca
    11281 ataaatctaa cgcaatacac ataatcctgg attcactttt gccgtacttc tgctttagtt
    11341 gctgcaattc tttttgtctc ttttcatcca acgtaacggg taaccgttta tttcctattg
    11401 caattggcat tagtactctc tcctttcatc gaaataaccc tatgaaaaat atggctgcaa
    11461 tcatcttgaa tagtagactc acaagggcta atacaaagcg ccaaaagatc aaacaaaata
    11521 ccagtattgc caaaaatact acgaagttta aatgcacagc tgcagcatgg cgcataagta
    11581 tttcgccaac aaataggact aacaatagag gaatgatttt attcatatgg atctccttct
    11641 ttctcttagg ttatcgggtg ttgcccgctt atttcgcacc acctaagcgg cgggggcggt
    11701 tggagcgatt ggtgcgaaat aagaaccatt cccttagtta ctaggttttt tgtccttatt
    11761 tcacacattc tcacttttcg gtataaatga ttattgcctt tttttccttt caaatcgttg
    11821 atatggcatt cgtttacggt caagttcatt ttggatttct tctgtatcgt cgtaagtata
    11881 ggtgtctaaa aaatcagtta aagtcgt
//
LOCUS       DAYYNQ010000092        11558 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000092 DAYYNQ010000000
VERSION     DAYYNQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.092"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHBJ1_002321"
     CDS             complement(105..629)
                     /locus_tag="ACHBJ1_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM9431237.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBJ1_002322"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHBJ1_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM9431238.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBJ1_002323"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHBJ1_002323"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM9431239.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHBJ1_002324"
     CDS             complement(2351..3031)
                     /locus_tag="ACHBJ1_002324"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM9431240.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHBJ1_002325"
     CDS             3157..4026
                     /locus_tag="ACHBJ1_002325"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431241.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHBJ1_002326"
     CDS             4052..4567
                     /locus_tag="ACHBJ1_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM9431242.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHBJ1_002327"
     CDS             complement(4614..5177)
                     /locus_tag="ACHBJ1_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9431243.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHBJ1_002328"
     CDS             complement(5232..5411)
                     /locus_tag="ACHBJ1_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431244.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHBJ1_002329"
     CDS             complement(5488..5997)
                     /locus_tag="ACHBJ1_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431245.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHBJ1_002330"
     CDS             complement(6093..6473)
                     /locus_tag="ACHBJ1_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9431246.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBJ1_002331"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHBJ1_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM9431247.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHBJ1_002332"
     CDS             complement(7736..9013)
                     /locus_tag="ACHBJ1_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431248.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBJ1_002333"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHBJ1_002333"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM9431249.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHBJ1_002334"
     CDS             complement(9935..10879)
                     /locus_tag="ACHBJ1_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM9431250.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..>11558)
                     /locus_tag="ACHBJ1_002335"
     CDS             complement(10881..>11558)
                     /locus_tag="ACHBJ1_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM9431251.1"
                     /translation="YLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLE
                     LLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRM
                     IFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFG
                     QVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKK
                     YYPGKGQ"
BASE COUNT         3288 a         2454 c         2042 g         3774 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagata
//
LOCUS       DAYYNQ010000093        11467 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000093 DAYYNQ010000000
VERSION     DAYYNQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.093"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..664)
                     /locus_tag="ACHBJ1_002336"
     CDS             complement(179..664)
                     /locus_tag="ACHBJ1_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM9431252.1"
                     /translation="MSRATQQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    754..1099
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1144..1785
                     /locus_tag="ACHBJ1_002337"
     CDS             1144..1785
                     /locus_tag="ACHBJ1_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM9431253.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1804..2439
                     /locus_tag="ACHBJ1_002338"
     CDS             1804..2439
                     /locus_tag="ACHBJ1_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM9431254.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2457..3272
                     /locus_tag="ACHBJ1_002339"
     CDS             2457..3272
                     /locus_tag="ACHBJ1_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9431255.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3591..5027
                     /locus_tag="ACHBJ1_002340"
     CDS             3591..5027
                     /locus_tag="ACHBJ1_002340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9431256.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5216..7648
                     /locus_tag="ACHBJ1_002341"
     CDS             5216..7648
                     /locus_tag="ACHBJ1_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9431257.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7878..8945
                     /gene="rlmN"
                     /locus_tag="ACHBJ1_002342"
     CDS             7878..8945
                     /gene="rlmN"
                     /locus_tag="ACHBJ1_002342"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9431258.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8935..9141
                     /locus_tag="ACHBJ1_002343"
     CDS             8935..9141
                     /locus_tag="ACHBJ1_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431259.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9138..10241)
                     /locus_tag="ACHBJ1_002344"
     CDS             complement(9138..10241)
                     /locus_tag="ACHBJ1_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9431260.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10793..11341
                     /gene="aac(6')"
                     /locus_tag="ACHBJ1_002345"
     CDS             10793..11341
                     /gene="aac(6')"
                     /locus_tag="ACHBJ1_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9431261.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3802 a         1940 c         2476 g         3249 t
ORIGIN      
        1 cttttttcgt agaatcaata aatatggatt tggctaacta tggaagaata ctcgtattag
       61 ttttcagcgt tcatactagt tgttacattg tttgattggc caaaagaatt tctgatatat
      121 tttgtgctaa ataaagaaaa agacgcttga attgggaaat aagaaaacta ctctatcatt
      181 acaaattttc ataaactggc agatgttcta agtaaacaag tatcttttca aagtcggaaa
      241 aatccataat caacgtagtg gtattgatat ttggatgaaa gcctacggta tcttgacgat
      301 caatagcagc atcgatcacg acttggattt ttttctcttt gtcgtgcatc aatgctaatg
      361 gagtcactgt acctgctgga acgcctagga ggtcaggaag ctgttcttca gaaacaaaag
      421 ataagcgctt ttcttttaat tgttcagcta gttgttttaa atcggcgtgc ttttcatcag
      481 gcaagatgac aagatagatt tgttttcctt ttcttgattt caataataga ttttttactt
      541 gcggaccagg tagggaaaaa gggacatttt tgactgacgt aatcgcagga tgatcgactt
      601 tttgaaaaga gatacccagt ttttttagta gttcataagc ttcttgttgg gttgcacgac
      661 tcatcgcgca tcaccctcct ttttttatta gtgtatcata aaaaagagtt gacaaatgaa
      721 aaataatcat gtaaattaca cgtaaagtta aaacaaatga catcgaaaag aagagtagcc
      781 gttaggtgtt tttagagagt ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca
      841 catcttggag tcgattttct gaagcgtata gtaggaaaat ccggggagcc cgttacagct
      901 gcggtcgttg ctagatgacc agataaggct ggcttttccg tgaggagtcg gtaaaaaaag
      961 gtggaaccac gtgattaacg tcctttgatc ttcggatcaa aggacttttt gttttcatct
     1021 gaacggcaac gactgtgtga ggttttttct gcaaggaaaa aataaacaga ggtggaacca
     1081 cgtataatac gtcctcttgg catttgccaa gaggattttt ttattttaaa ggaggaaaag
     1141 aagatggagt atttgattga agttatgccg caattattag taggagcact cacaacattg
     1201 aaattatttt tatttacctt gattggctca ttaccattag ggattgtttt agcattagga
     1261 ttgtctttgc attttaagcc gctacgctac ttgcttcaaa catatgtatg ggttatgcgg
     1321 ggaacaccgc tgttgttgca actgattttt attttttatg ggttgccgac gattgggatc
     1381 gtttttgatc gtttcgaagc agcactcatt gcctttatct taaactatgc agcttatttt
     1441 gcagagatct ttcgtggagg gatacaatcg attcctcaag gtcaatatga agcagcgcaa
     1501 gttttgcaat taagtccctt tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag
     1561 attgttttac cttctgtagg gaatgaagtt atcaacttgg tcaaagattc ttccttgatc
     1621 tatatattag gtttaggtga cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg
     1681 actttactgc ctttgctagg cgtggcaatc atctatctta cattgactgg tattctaaca
     1741 atcgtaatga aacaagttga aggaaaatta aattattacc gctaactgat aggaggaaga
     1801 aatatgattg aattgaagaa agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat
     1861 ttgaatctaa tcattccaga tgggaaaaca ttagctatcg taggtccttc cggtggcggt
     1921 aaaaccacgc ttttacgtat cttggctggc ttagaaagtt ctgacagcgg agaattttat
     1981 ttagacggtg taccgttcaa cccattcgag cttaaaatga aagaacaagt tgtaggagtc
     2041 gtattccaag acttccagct atttccacat ctgaatattt ttgaaaacat taccttagca
     2101 ccaaaaatgg tattgaaaca gacaaaagaa gaatacacca aaaaagcaga agaattagcg
     2161 gcttcattag ggattagtga tttattgaat atctatcctt atcagctttc tggcggacaa
     2221 aaacagcggg tagcgattgc cagagcattg gctatgaatc cacgggtatt ggcttatgat
     2281 gaaccaacaa gtgctttaga tcctgaatta cgccagcaag tcgagcgttt gatcctatca
     2341 ttgaaagaag atggggtaac ccaaattatt gtcacccatg atttagtatt tgcagaaaat
     2401 gtcggtgatc aattactaaa agtttcccca attcattaag aaaggagaag atagagatga
     2461 aaaaaaccag ttttctattc gctttgatcg caggtcttct tctttttgca ggctgttcga
     2521 acaaaaaaac ttcagcagat aattgggcga ctatcaacga agagaaaaaa atcgttatag
     2581 gtttagacga tacatttgtt cctatgggat ttagagataa agacgggaag ctcacaggat
     2641 ttgatattga tttagccaaa gcagtctttg aacaatatgg gattacggta gactttcagc
     2701 ccattgactg gtcaatgaag gaatttgaac tgaacaatgg aacgatcgat ttgatatgga
     2761 acggctattc caaaacgccg gcaagagaaa agaaagtgca atttacaaaa ccttatatgg
     2821 aaaacgatca agtgctgatc acacctaaag aatcaggaat cacaaacttt ggacaaatga
     2881 ataataagag attaggtgcg caaaatgggt catctggtta tgatgtattt acgaaacaac
     2941 cagaagtatt aaaagagata gtcaaaaacc aagatgctgt tttgtatgat agttttaatg
     3001 aagcattgat cgatctgaaa agcagacggg tcgatggtct gctgatggat aaagtatacg
     3061 cagactatta tttgaaacaa agaaacgaac tagaagactt caacataaca aaaggttctt
     3121 atgagagtga ggattttgca gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa
     3181 tcaatgaagc cctagatcaa ctagagaaaa caggtgaatt ccaaaaaata tccgataaat
     3241 ggtttggcga agatgttacg ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg
     3301 tcacagtccc tttttccgaa taaacagtgt tctcctgcca actccttgtg actcggagct
     3361 gaatggcagg ctcacaacct cttggtaaac ggtgttcaaa agccagtttt tcggtattaa
     3421 gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa atttttctta ctactcgaaa
     3481 ctaagcgact ttttcacaac ctcctttaca taattttatt ttgttgtgga aggggtttcg
     3541 tgttattcta aaagaggaac gattgaaggt tataggaaag gaagagatga atggaacaca
     3601 aagaacttac ctcatttcca aaagattttc tgtggggctc tgcttctgca gcgtatcaag
     3661 tagaaggagc ttggcaagaa gatgggaaag gagaatcagt ctgggatcga tttgtacgga
     3721 tacctggaaa gacatttaaa ggaacgaacg gtgatttggc agtagaccac tatcatcgtt
     3781 ataaagaaga tattgcttta atgaaagaac aaggtctgaa agcttaccgc ttctctgtag
     3841 cttggacacg gatttttcct aatggacgag gagaagtcaa tcaagctggg ctcgcttttt
     3901 atgagagatt gatcgacgaa ttgattgaaa acgatattga acctatgttg actttatatc
     3961 attgggatct tccacaagca ttgcaagatg aatataatgg ttgggaatca cgtcagatca
     4021 ttgaagattt cactcattat gcagaaacat tatttgaggc attccgcggg aaagtccatt
     4081 actggattag tttgaatgaa caaaatatct tcacttcatt aggttatttg ttagcagcac
     4141 atccgccagg tgtgacagac ccgaaacgaa tgtatgaagt taaccatatt gctaatttag
     4201 caaatgcatc tgtcatcaat aaatttcacg agttagaaat gcctggaaaa atcggaccaa
     4261 gctttgcgta tacgccaaat tatccaattg acagtaatcc tgaaaatgtt ctggctgctg
     4321 aaaatgcaga agacctgatg gccaattatt ggatggatgt ctatatgtgg ggaaaatatc
     4381 cgattgctgc catgaggttc ttagaagaaa aaggatgggc accaacgatt gaagctggcg
     4441 atgcagaatt gctagaatct gctaaaccag attttcttgg gatcaattac tatcaaacgg
     4501 cgacgaatgc attcaatcca ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa
     4561 aaaaaagttc atcagaagaa actggagttc ctggaatgta taaaaaagtt gagaatccat
     4621 ttgtcgaacg aacaaattgg gactgggaga ttgacccaga aggattaagg atcggactgc
     4681 gtcgtatcac aagccgttac cgcatgccgg tactgattac ggaaaatggc ttaggagagt
     4741 atgacaagtt gacagaagat aaacaaatcc atgacgacta tcgaatcaat tacttacaaa
     4801 gccacgtcaa agccatcaaa gaagcaattt cggatggagc agaagtccta ggctattgca
     4861 cctggtctta tacagatttg cttagctggc tgaacggtta tcaaaagcga tatggatttg
     4921 tatatgttga tcaagacgaa acacaagaag gatctttgga acgatataag aaagacagtt
     4981 attactggta ccaaaaactg attgaagaaa atggagagaa cctttagttc acatacttaa
     5041 aacaagaagg tacggctgga tatggccgtg ccttttcttg ataaaatcat ttagaaatta
     5101 agaaaaactg taaaataagc tgaaaaatga aagaaagcgt cagttctttc aaacaaaact
     5161 ttagttccct tacttttttt tgtataatga aacttgatga aatgagggat aataaatggc
     5221 acaaaagaaa cggcctacta aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca
     5281 gcagcaggaa caaatgctga cgattggtgt tgggtttgcc ttgattttat ttgctgtctt
     5341 cggcttttta aaattaggtt ttttaggaat attgattgca aacggctttc ggatcattgc
     5401 aggaaatact taccaaatcc tctgtctttt attagccgta ttaggatttt ggattgtcat
     5461 taagaataca gagttttcaa ttggtaaaaa ccgacgctgg tttggcggca tactctttta
     5521 tttcggtatc ttattattgt tacatgccca tttatttggc aaactccata ctggggaacc
     5581 aaatatcatg gggacgacat gggatttgtt agccagtgat ataaaacaaa gtcaagtaga
     5641 caataacgtt ggtggtggga tgattggagc aatcctttat cattttactt acttcttgat
     5701 tgcacagcct ggaagttatt tagcagcagt gctattactg gcaggaggag cattcctgtt
     5761 cagtaatctg gaaggctatc agctgttaaa tggtatccaa tcaattggag aacgagtaca
     5821 ggaattattg gaaggtgatc ctgccaagca agctcgaaag caagcggcaa aggaagagag
     5881 aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga
     5941 atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga
     6001 accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa
     6061 tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc
     6121 agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga
     6181 agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc
     6241 aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca
     6301 aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt
     6361 cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac
     6421 agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg
     6481 taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga
     6541 aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga
     6601 tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg
     6661 ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg
     6721 tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat
     6781 gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca
     6841 agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg
     6901 agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac
     6961 gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat
     7021 ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc
     7081 ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat
     7141 caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac
     7201 gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc
     7261 aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga
     7321 acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc
     7381 gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga
     7441 agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt
     7501 tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt
     7561 tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga
     7621 aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata
     7681 accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt
     7741 ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt
     7801 atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga
     7861 aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa
     7921 tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt
     7981 tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg
     8041 aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt
     8101 cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac
     8161 tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg
     8221 gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa
     8281 ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg
     8341 tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg
     8401 actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag
     8461 tttccactag tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca
     8521 atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca
     8581 accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa
     8641 accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg
     8701 cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc
     8761 catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga
     8821 gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta
     8881 cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca
     8941 aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag
     9001 gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg
     9061 acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg
     9121 ggggttttag caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca
     9181 tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc
     9241 acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg
     9301 acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag
     9361 attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg
     9421 acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat
     9481 gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta
     9541 acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct
     9601 ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt
     9661 cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct
     9721 ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt
     9781 gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg
     9841 ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg
     9901 ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca
     9961 cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac
    10021 tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt
    10081 tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca
    10141 atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct
    10201 gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt
    10261 cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta
    10321 gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc
    10381 aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt
    10441 gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa
    10501 aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta
    10561 aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct
    10621 ttcctgatct ttcctatttt gaataaagtg tttctgtata attttttatt ttataagtaa
    10681 gataaataat gaaagaatag agatactgat tggtagctaa agatttttga gcgagaacat
    10741 ttcgtttttt caaaaggact gttagaataa gcaagaacaa gggggaagac gtatgataat
    10801 cagtgaattt gaccgtaata atccagtatt gaaagatcag ctttctgatt tactgagact
    10861 gacttggccg gaagaatatg gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc
    10921 agaacgaatc gcggtagcag cggtagacca agatgagtta gtaggattta ttggtgcaat
    10981 ccctcaatac ggtatcacag gttgggaatt gcatccatta gttgtagaaa gctcccgacg
    11041 aaagaaccaa ataggtactc gattagtcaa ttacttagaa aaagaagtag cttccagagg
    11101 aggaatcacg atttatttag gtacggatga tttagaccat ggaacaacgt taagtcaaac
    11161 ggacctgtat gagcatacat ttgataaagt ggcttccatc cagaaccttc gtgaacatcc
    11221 gtatgaattc tatgaaaaat taggttataa aatcgtaggt gtcttaccaa atgcaaatgg
    11281 ctgggacaaa ccggatattt ggatggcaaa aacgattatt cctcgaccaa attctcaata
    11341 ataaaaagag gttgtgacag aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa
    11401 ttacttcttg aatttttgtg aaattccaac ttatttccga agaagctact tttggaacac
    11461 cgtgtac
//
LOCUS       DAYYNQ010000094        11396 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000094 DAYYNQ010000000
VERSION     DAYYNQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.094"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACHBJ1_002346"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACHBJ1_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACHBJ1_002347"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACHBJ1_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACHBJ1_002348"
     CDS             2093..2254
                     /locus_tag="ACHBJ1_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431262.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3334
                     /locus_tag="ACHBJ1_002349"
     CDS             2738..3334
                     /locus_tag="ACHBJ1_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFM9431263.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3315..3761
                     /locus_tag="ACHBJ1_002350"
     CDS             3315..3761
                     /locus_tag="ACHBJ1_002350"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM9431264.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            4104..4427
                     /gene="relB"
                     /locus_tag="ACHBJ1_002351"
     CDS             4104..4427
                     /gene="relB"
                     /locus_tag="ACHBJ1_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM9431265.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            4747..4974
                     /locus_tag="ACHBJ1_002352"
     CDS             4747..4974
                     /locus_tag="ACHBJ1_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM9431266.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /gene="hemW"
                     /locus_tag="ACHBJ1_002353"
     CDS             4990..6156
                     /gene="hemW"
                     /locus_tag="ACHBJ1_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM9431267.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="ACHBJ1_002354"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="ACHBJ1_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM9431268.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="ACHBJ1_002355"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="ACHBJ1_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM9431269.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="ACHBJ1_002356"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="ACHBJ1_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM9431270.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACHBJ1_002357"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACHBJ1_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM9431271.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4004 a         1834 c         2333 g         3225 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctgg
//
LOCUS       DAYYNQ010000095        11217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000095 DAYYNQ010000000
VERSION     DAYYNQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.095"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHBJ1_002358"
     CDS             6..305
                     /locus_tag="ACHBJ1_002358"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9431272.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHBJ1_002359"
     CDS             378..1739
                     /locus_tag="ACHBJ1_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM9431273.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHBJ1_002360"
     CDS             1761..2021
                     /locus_tag="ACHBJ1_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431274.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHBJ1_002361"
     CDS             2035..2787
                     /locus_tag="ACHBJ1_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM9431275.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHBJ1_002362"
     CDS             complement(2881..3486)
                     /locus_tag="ACHBJ1_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9431276.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHBJ1_002363"
     CDS             complement(3539..4513)
                     /locus_tag="ACHBJ1_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM9431277.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHBJ1_002364"
     CDS             complement(4891..5610)
                     /locus_tag="ACHBJ1_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9431278.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBJ1_002365"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHBJ1_002365"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9431279.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHBJ1_002366"
     CDS             7161..8558
                     /locus_tag="ACHBJ1_002366"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM9431280.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHBJ1_002367"
     CDS             8639..9079
                     /locus_tag="ACHBJ1_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9431281.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHBJ1_002368"
     CDS             9227..9553
                     /locus_tag="ACHBJ1_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9431282.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHBJ1_002369"
     CDS             9554..10303
                     /locus_tag="ACHBJ1_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM9431283.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHBJ1_002370"
     CDS             complement(10383..10838)
                     /locus_tag="ACHBJ1_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9431284.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3558 a         1960 c         2302 g         3397 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagt
//
LOCUS       DAYYNQ010000096        10763 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000096 DAYYNQ010000000
VERSION     DAYYNQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.096"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            362..631
                     /gene="rpsN"
                     /locus_tag="ACHBJ1_002371"
     CDS             362..631
                     /gene="rpsN"
                     /locus_tag="ACHBJ1_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM9431285.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            646..825
                     /gene="rpmF"
                     /locus_tag="ACHBJ1_002372"
     CDS             646..825
                     /gene="rpmF"
                     /locus_tag="ACHBJ1_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM9431286.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            857..1234
                     /locus_tag="ACHBJ1_002373"
     CDS             857..1234
                     /locus_tag="ACHBJ1_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431287.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1255..1404
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_002374"
     CDS             1255..1404
                     /gene="rpmG"
                     /locus_tag="ACHBJ1_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM9431288.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1428..2411
                     /locus_tag="ACHBJ1_002375"
     CDS             1428..2411
                     /locus_tag="ACHBJ1_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9431289.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2441..2563
                     /locus_tag="ACHBJ1_002376"
     CDS             2441..2563
                     /locus_tag="ACHBJ1_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM9431290.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2581..4104
                     /locus_tag="ACHBJ1_002377"
     CDS             2581..4104
                     /locus_tag="ACHBJ1_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM9431291.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4128..4367
                     /locus_tag="ACHBJ1_002378"
     CDS             4128..4367
                     /locus_tag="ACHBJ1_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431292.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4560..5021)
                     /locus_tag="ACHBJ1_002379"
     CDS             complement(4560..5021)
                     /locus_tag="ACHBJ1_002379"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9431293.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5216..5434)
                     /locus_tag="ACHBJ1_002380"
     CDS             complement(5216..5434)
                     /locus_tag="ACHBJ1_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431294.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5552..7168
                     /locus_tag="ACHBJ1_002381"
     CDS             5552..7168
                     /locus_tag="ACHBJ1_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM9431295.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7409..8749)
                     /locus_tag="ACHBJ1_002382"
                     /pseudo
     CDS             complement(7409..8749)
                     /locus_tag="ACHBJ1_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9187..9333)
                     /locus_tag="ACHBJ1_002383"
     CDS             complement(9187..9333)
                     /locus_tag="ACHBJ1_002383"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431296.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9372..9566)
                     /locus_tag="ACHBJ1_002384"
     CDS             complement(9372..9566)
                     /locus_tag="ACHBJ1_002384"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9431297.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9476..9808)
                     /locus_tag="ACHBJ1_002385"
     CDS             complement(9476..9808)
                     /locus_tag="ACHBJ1_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9431298.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10322..10513)
                     /locus_tag="ACHBJ1_002386"
     CDS             complement(10322..10513)
                     /locus_tag="ACHBJ1_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431299.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10607..>10763)
                     /locus_tag="ACHBJ1_002387"
     CDS             complement(10607..>10763)
                     /locus_tag="ACHBJ1_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9431300.1"
                     /translation="PLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYK
                     KIQKMLV"
BASE COUNT         3581 a         1706 c         2089 g         3387 t
ORIGIN      
        1 caaagtttta aaaaattggc gataaaccag ttaaacataa aagattgaat aaaaagttat
       61 agtttctttc atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt
      121 tgattggact aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa
      181 atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg
      241 agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg
      301 tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta
      361 tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta
      421 tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc
      481 aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg
      541 gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt
      601 gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca
      661 agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca
      721 gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa
      781 gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt
      841 ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta
      901 ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga
      961 gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt
     1021 caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat
     1081 ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt
     1141 tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc
     1201 acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt
     1261 caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat
     1321 aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg
     1381 gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag
     1441 ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc
     1501 aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg
     1561 ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat
     1621 gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa
     1681 tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc
     1741 cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg
     1801 atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag
     1861 cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt
     1921 tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc
     1981 gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta
     2041 atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc
     2101 atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa
     2161 aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt
     2221 atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg
     2281 taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc
     2341 taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg
     2401 aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc
     2461 tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat
     2521 tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga
     2581 atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc
     2641 tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca
     2701 acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat
     2761 ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca
     2821 aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca
     2881 aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg
     2941 attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat
     3001 gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc
     3061 aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa
     3121 aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg
     3181 aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga
     3241 ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta
     3301 gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat
     3361 gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg
     3421 aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta
     3481 atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt
     3541 ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat
     3601 gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta
     3661 aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca
     3721 gctgccgagt acaaagatta ttatactact ggctataaga cagatattga tactatcaac
     3781 attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa
     3841 tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt
     3901 gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt
     3961 gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc
     4021 tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt
     4081 gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag
     4141 tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc
     4201 aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat gttagtattg
     4261 acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg
     4321 gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg ataataccag
     4381 agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca
     4441 aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt
     4501 tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc
     4561 tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca
     4621 ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta
     4681 actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca
     4741 tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg
     4801 gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa
     4861 gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca
     4921 tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg
     4981 acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta
     5041 ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg
     5101 ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc
     5161 gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga
     5221 atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt
     5281 gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa
     5341 tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc
     5401 gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct
     5461 cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag
     5521 tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac
     5581 tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg
     5641 aaagaaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt
     5701 cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat
     5761 ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga
     5821 cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc
     5881 aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc
     5941 cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat
     6001 tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt
     6061 aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac
     6121 caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat
     6181 taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat
     6241 agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca
     6301 tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct
     6361 tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc
     6421 aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg
     6481 tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg
     6541 tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga
     6601 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
     6661 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
     6721 tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
     6781 tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct cttttattcc
     6841 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
     6901 catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
     6961 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
     7021 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
     7081 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
     7141 agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
     7201 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
     7261 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
     7321 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
     7381 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
     7441 ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata
     7501 aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta
     7561 ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc
     7621 gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca
     7681 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa
     7741 cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata
     7801 gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
     7861 gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct
     7921 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag
     7981 cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
     8041 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt
     8101 cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
     8161 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
     8221 agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca
     8281 aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
     8341 gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt
     8401 gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag
     8461 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt
     8521 tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt
     8581 cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa
     8641 caaaacatgt ttcgtcattg attcgctctt tgcgaaaggc atcttcatga aagataagat
     8701 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
     8761 cctttgaatg gttttcgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta
     8821 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
     8881 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
     8941 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
     9001 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
     9061 attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag
     9121 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
     9181 ttctccttaa taaatttgta atggacggac gacaatctct tttttcatgt gtgaatctac
     9241 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
     9301 gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc
     9361 aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa
     9421 gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca
     9481 acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg
     9541 tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt
     9601 caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt
     9661 tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt
     9721 cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt
     9781 taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat
     9841 agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga
     9901 agttgataga taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca
     9961 aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg
    10021 cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac
    10081 ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta
    10141 tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct
    10201 atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca
    10261 aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga
    10321 gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat
    10381 aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc
    10441 ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt
    10501 ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg
    10561 aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt
    10621 tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca
    10681 gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt
    10741 gttcaggttc tttcaacaaa gga
//
LOCUS       DAYYNQ010000097        10499 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000097 DAYYNQ010000000
VERSION     DAYYNQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.097"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACHBJ1_002388"
     CDS             33..656
                     /locus_tag="ACHBJ1_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431301.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBJ1_002389"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACHBJ1_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM9431302.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACHBJ1_002390"
     CDS             2152..3033
                     /locus_tag="ACHBJ1_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431303.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACHBJ1_002391"
     CDS             3036..3806
                     /locus_tag="ACHBJ1_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431304.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBJ1_002392"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACHBJ1_002392"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM9431305.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACHBJ1_002393"
     CDS             5117..6280
                     /locus_tag="ACHBJ1_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9431306.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACHBJ1_002394"
     CDS             6397..6807
                     /locus_tag="ACHBJ1_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM9431307.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACHBJ1_002395"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACHBJ1_002395"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM9431308.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACHBJ1_002396"
     CDS             9380..9664
                     /locus_tag="ACHBJ1_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431309.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACHBJ1_002397"
     CDS             9824..10060
                     /locus_tag="ACHBJ1_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9431310.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACHBJ1_002398"
     CDS             10057..10455
                     /locus_tag="ACHBJ1_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM9431311.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3661 a         1766 c         2224 g         2848 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcatta
//
LOCUS       DAYYNQ010000098        10322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000098 DAYYNQ010000000
VERSION     DAYYNQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.098"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..324
                     /locus_tag="ACHBJ1_002399"
     CDS             175..324
                     /locus_tag="ACHBJ1_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM9431312.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            335..1849
                     /gene="dltA"
                     /locus_tag="ACHBJ1_002400"
     CDS             335..1849
                     /gene="dltA"
                     /locus_tag="ACHBJ1_002400"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM9431313.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1846..3051
                     /gene="dltB"
                     /locus_tag="ACHBJ1_002401"
     CDS             1846..3051
                     /gene="dltB"
                     /locus_tag="ACHBJ1_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM9431314.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3096..3329
                     /gene="dltC"
                     /locus_tag="ACHBJ1_002402"
     CDS             3096..3329
                     /gene="dltC"
                     /locus_tag="ACHBJ1_002402"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM9431315.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3332..4600
                     /gene="dltD"
                     /locus_tag="ACHBJ1_002403"
     CDS             3332..4600
                     /gene="dltD"
                     /locus_tag="ACHBJ1_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM9431316.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4741..5823
                     /locus_tag="ACHBJ1_002404"
     CDS             4741..5823
                     /locus_tag="ACHBJ1_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM9431317.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5823..6626
                     /locus_tag="ACHBJ1_002405"
     CDS             5823..6626
                     /locus_tag="ACHBJ1_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM9431318.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6696..7436
                     /locus_tag="ACHBJ1_002406"
     CDS             6696..7436
                     /locus_tag="ACHBJ1_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM9431319.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7447..8451
                     /locus_tag="ACHBJ1_002407"
     CDS             7447..8451
                     /locus_tag="ACHBJ1_002407"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM9431320.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8496..>10322)
                     /locus_tag="ACHBJ1_002408"
     CDS             complement(8496..>10322)
                     /locus_tag="ACHBJ1_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431321.1"
                     /translation="IYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQM
                     KEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQ
                     WITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPK
                     VYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCF
                     ALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFC
                     TFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACT
                     PWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQ
                     VDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKIL
                     EEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLI
                     HALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQ
                     ERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3319 a         1703 c         2086 g         3214 t
ORIGIN      
        1 ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat
       61 aaaagctatg tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt
      121 ctttttgtca aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac
      181 aaatggcgtt ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt
      241 tatagtgcag tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga
      301 acttttatct ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc
      361 aatcgatgat tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca
      421 tacttttggt gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt
      481 agaaggcgat ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt
      541 tctaggggtt gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga
      601 gcgtatccta tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg
      661 gatagaagat attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa
      721 gataccagta ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat
      781 ttttacttct ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt
      841 cagctttgtt caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc
      901 ccaagcgcca ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg
      961 cggaatgctg aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac
     1021 attgccagcc ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt
     1081 gatggaacct acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg
     1141 agaagaattg acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat
     1201 atataatact tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca
     1261 gcatgtacta gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt
     1321 ttctattgtc caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc
     1381 aggaccaagc gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt
     1441 ttgtcaaaat gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg
     1501 gcttttattt tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga
     1561 actcggggat atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt
     1621 accaaaatac caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa
     1681 aacgaaacag ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt
     1741 aatggattat atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa
     1801 tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat
     1861 atgatccctt atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc
     1921 gttctatctt tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt
     1981 ttctttttat ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg
     2041 tatgttttgt ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat
     2101 cagacaggat ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa
     2161 gtgactcctt tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat
     2221 ttaaccttta aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat
     2281 aagccatttc gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc
     2341 gatcgctata ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt
     2401 gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc
     2461 ggttattatt ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt
     2521 ggtatttcat gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat
     2581 tttgctggct atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca
     2641 atcaatttca ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat
     2701 atgacgctgt ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg
     2761 aagaaaaaat tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt
     2821 cttctaatgg gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat
     2881 gcttgtctga tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag
     2941 ttgccttcta acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt
     3001 tttagcttcc tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg
     3061 acatattaat ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct
     3121 tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg
     3181 aagggttgct tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg
     3241 atatccaagt ccctgtatct gaattcgagc gagaggattg gtgcacaccg aataaaatca
     3301 ttcaacaagc aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg
     3361 cccggttgtt ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga
     3421 tatctctaat ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa
     3481 aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc
     3541 aagtgaatta agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg
     3601 aaactatcgt ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat
     3661 gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca
     3721 gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca
     3781 aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag
     3841 aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac
     3901 gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa
     3961 catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa
     4021 aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt
     4081 agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt
     4141 ttatacgcat cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga
     4201 ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa
     4261 ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg
     4321 actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca
     4381 aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga
     4441 cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt
     4501 agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc
     4561 ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc
     4621 tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct
     4681 gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat
     4741 ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga
     4801 aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt
     4861 ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc
     4921 tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga
     4981 ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt
     5041 gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat
     5101 gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt
     5161 ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact
     5221 ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat
     5281 aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac
     5341 ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga
     5401 acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga
     5461 gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt
     5521 ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta
     5581 gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca
     5641 ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt
     5701 tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta
     5761 gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa
     5821 taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa
     5881 atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc
     5941 agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga
     6001 gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc
     6061 tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa
     6121 agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca
     6181 acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc
     6241 ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct
     6301 ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg
     6361 gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat
     6421 gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc
     6481 tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg
     6541 tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag
     6601 gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt
     6661 ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct
     6721 taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt
     6781 tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc
     6841 cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg
     6901 gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa
     6961 tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa
     7021 aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta
     7081 tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga
     7141 aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag
     7201 atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc
     7261 atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac
     7321 ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag
     7381 caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg
     7441 aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa
     7501 caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa
     7561 ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg
     7621 gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat
     7681 tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt
     7741 gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa
     7801 ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat
     7861 gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa
     7921 gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc
     7981 cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca
     8041 gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta
     8101 aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat
     8161 tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa
     8221 cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa
     8281 gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg
     8341 gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag
     8401 aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa
     8461 atagcggaat ccacatatat ggattccgct attttttata caggattcgg gtaatttcga
     8521 gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag tgttcctgtg
     8581 taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc atctaattct
     8641 tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc gcaacaaatg
     8701 acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag tggattttcg
     8761 atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg agccagatcg
     8821 tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg aatattccgt
     8881 ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg gttgcaaaaa
     8941 gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata gtttttagct
     9001 tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac aaattccagg
     9061 cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg ctccaacaaa
     9121 tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt taagatgtga
     9181 tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat cgtagaccaa
     9241 ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca atggataatg
     9301 gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt atatgcttcg
     9361 ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt agcaatcggc
     9421 attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc gataaaaatc
     9481 aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat tcggctccaa
     9541 tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa aaaagaatga
     9601 acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc aaatcctaat
     9661 gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg tttatacatc
     9721 aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat catcgtcaat
     9781 acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt tcctagataa
     9841 actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag ggtcaatttt
     9901 tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg attgctttct
     9961 tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt aatccattga
    10021 tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt cgtttccata
    10081 atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct gtcaaactta
    10141 aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt catttgattg
    10201 ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa aaataacaat
    10261 gataatataa tgattatgct ccactttata cgtaaataaa aagattcttt tttattatat
    10321 at
//
LOCUS       DAYYNQ010000099         9706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000099 DAYYNQ010000000
VERSION     DAYYNQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.099"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..197)
                     /locus_tag="ACHBJ1_002409"
     CDS             complement(<1..197)
                     /locus_tag="ACHBJ1_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431322.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(213..827)
                     /locus_tag="ACHBJ1_002410"
     CDS             complement(213..827)
                     /locus_tag="ACHBJ1_002410"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM9431323.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(974..1657)
                     /locus_tag="ACHBJ1_002411"
     CDS             complement(974..1657)
                     /locus_tag="ACHBJ1_002411"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM9431324.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1766..2224)
                     /locus_tag="ACHBJ1_002412"
     CDS             complement(1766..2224)
                     /locus_tag="ACHBJ1_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM9431325.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2308..4071)
                     /locus_tag="ACHBJ1_002413"
     CDS             complement(2308..4071)
                     /locus_tag="ACHBJ1_002413"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM9431326.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4292..5752)
                     /locus_tag="ACHBJ1_002414"
     CDS             complement(4292..5752)
                     /locus_tag="ACHBJ1_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431327.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5960..6886)
                     /gene="glsA"
                     /locus_tag="ACHBJ1_002415"
     CDS             complement(5960..6886)
                     /gene="glsA"
                     /locus_tag="ACHBJ1_002415"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM9431328.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6909..8336)
                     /locus_tag="ACHBJ1_002416"
     CDS             complement(6909..8336)
                     /locus_tag="ACHBJ1_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9431329.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAYLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8367..9581)
                     /locus_tag="ACHBJ1_002417"
     CDS             complement(8367..9581)
                     /locus_tag="ACHBJ1_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM9431330.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2877 a         2026 c         1615 g         3188 t
ORIGIN      
        1 aagtaattaa acaatccgct aatcgtttga tctggcattt ctatttcctt accatctaaa
       61 gtaacagtag taacgacaaa atggtcttgc aatggcggaa cttctaagta ctgcccttgc
      121 agaaaatcat tcactgtttc atattctttt ttcatttctt ttgaatctgt cgatggttta
      181 tagcttattt ttaacataac tagtgctcct ttttattcgg gacgtgcaat cgtgaaaatt
      241 tctcctattg ctgcataatc atttcctgcc aaacggctta cagcattcaa ttttcttgga
      301 tcgatccttc ctgtcttatc atcatagact gtttcattta aatgatattt ctgtacttct
      361 agcaaaaata aatcagctgt tactttattt ttattccgta tctcgacatg ttcatataag
      421 gatgtttcaa aacgaacttt tgcttctgtc aatgctggta ctgaaatgat tactgaatca
      481 gttaaagtca agtcagtcag gtacagttcg ctttcctcat taggcagatt ggctgctgtt
      541 ttatttactt cttctacgtt gtcctcatca aggatatgga taacagcttc tcttgtctca
      601 agaatattct ttgctgtgtc ttttactgca tcttgttttc tctggatcgc caaggataag
      661 atgggtcgat tcgaactgac gatattgaag taactgaagg gtgcgatatt cactatatct
      721 tttgctgatt tagtcgttac tactgctact ggtctaggga tgactgaacc aatcagcaac
      781 ttgtaatttt cacgttctgt caattctgaa ggactccgtt caaacatggt ttcgccttct
      841 ttctataaaa ttcgttaaaa agcagatctt gaagacaaaa tgaaaagtaa tgcttttttc
      901 atcttcttag aatctgcttt aaactatcta tcaatttttc ttttttgtca agaatctgtg
      961 ccgtgcgtgt tttttagtta tttgctttac ttgcggcttc gacatatctt ttagtgatca
     1021 attgcggacg ggtgataata gatccaacta cgactgaaaa agcacctaat tcatatgctt
     1081 tctctacttg ttcaggagag tcagtatgtc cttccataat tactgggata tcgacttgtt
     1141 ctatgatttg attcaataaa tcaaaatcag gtgtctgcgt ctttcgtgaa tattctgtat
     1201 agcctgctaa agtcgtagac aaaagatcaa aaccatcttc ttgcgcttga agcgcttcct
     1261 caaaagtcga aatatctgcc atagctaacc gaccgtgttc gtgaatcatt tggacaagtt
     1321 cagcagttgt tttttctgtc ggacgtttac gagaagttgc atccagtgca atgatttctg
     1381 cttgcgtctc caaaagttct tccacttctt tttctgtcgc agtaataaac acttctgacc
     1441 catcgtaatc tcttttgata atccctatta ccggtaaatc aacgattcgc ttgatttcat
     1501 tgatatcttc tgtcgtattt gctcgaatac caactgctcc gccttctttt gcagccaaag
     1561 ccattctccc cataataaaa gaactgtgta gcggctcgtt ttccaaagct tgacaagaaa
     1621 cgacgactcc gcctttgatt tgttccagtg tatccacaat atctcctcca gtaatgttat
     1681 ataaaaatac agcttcatat aaaaatgtaa cccttttcta ttcaggagac aataatgagc
     1741 tatttctttc agcagacctt tcttactaaa agaaaattac ttcaaattct tcgtatacga
     1801 ccagcttctc ttctgtaaat tctatgttca acgcttcttt ataagcatgt ttaaaaaact
     1861 cagcatggac tttccgccaa taagctaatg tccgatcccc ttctccttct ttccaagcat
     1921 gctctttcgt gacttcacta aaaggcaaga tggtcacatt tatatttttg atgatacaaa
     1981 ctggttcatt ttttccattc aggaccacgt ctaattgacc aacttttggt aaaggctcat
     2041 tctcttgttt atacagatcg taaccacttg atgtaccagt ctttatcttt tttacaacta
     2101 gttcgctcaa ctcgtctgcc attttttctg tatccccaaa tgaaaagaaa cctaattttt
     2161 ctgattgata actaggatat ttttcttgga actttttgac atattcttct acttgctgat
     2221 acataacatc cacccctttg ctttttataa tgaaaaaacc atccagatac tagtataaac
     2281 aattatctgg atggtgagca taatgaatta ttgaatattc aagacacgca gactatcttc
     2341 aacgatttgt tttggtgtta aatgataacg ttcataaagc tgccctacag gaacgctatc
     2401 cgtgaattct ttctctgcac caaaatttaa tacgttcatt gaagaatcac cataataacg
     2461 actgatattt tcgccaaatc ctcctgacaa actaccatct tctaatgtga tgactaattc
     2521 gtgtgtttct tttaatttat ctaatgtttc tacgtcattt tctgtgatcg ttcctggatt
     2581 gatcaacgtt gcttcgattc ctttttttct aaaagctttt actgtttctt ttcctaattc
     2641 aagaaaaccg cctaaagcta aaattgctac ttttcctcca ttatttactg tttgataggc
     2701 tggaatacta taatcatttg cgatagctgt accatgttct acaccatgtg taggaattcg
     2761 gattgctaca ggctgcgctg tttgttcgaa cgcccaatct agcatagaga tcatatcctc
     2821 ttttgttgct ggagcaagat atttgatgtt cgggatactt gataagtaag gaatgtcaaa
     2881 tgttccttgg tgtgttcggt cactagctga gatcgttccg ccaccgacta aaataatcac
     2941 tggattctcg ttgatcgcta aatcatgtga taattgatca tatgcccgtt gcaagaaagt
     3001 acttgattgg aagacgactg gacgtactcc ctgagaagcc aaagcagaag cgaaagtgat
     3061 agagtgctgt tcagcgatcc ccacgtcaaa ataatgatct ggatgttttt cttggaatgt
     3121 tttcagtcca aaggcgcctg gtatcgctgc attgactgcg gcaataggag ctgtacttgt
     3181 ttgcattttc ttatccaaat aatccatgat gatcatatta aatgtttctc cagaagttac
     3241 ttgctttgcc tccccggtgg acagatcaaa tggtacatgc caatgatatt tcattttatt
     3301 atcgattgcc ggctgataac cgcgaccttt ttccgtatgg acgtgaagta caatcggatg
     3361 atcaatatct tttacttctt caaataaacg gatcaccgtt tccagatcgt ttccattatc
     3421 cacgtaacga taatcaaatc ccattgcttt aaataggtta ttgctgcttt taccgttggt
     3481 ttgacgaagc tcagctaagt ttttatataa actgccatga ttctcatcaa tagacatctc
     3541 attatcgttt actaatacga ttagattgga ttgtaaaaca ccaccattgt tcaatccttc
     3601 aaaagctaag ccaccactta gggagccgtc accgataaat gctaccacat ttcctgattc
     3661 tttcttcaga tctctcgctt tagctaaacc tgttgctaag ctgatagatg tagatgtgtg
     3721 ccccactgta aaatagtcat gtttactttc atgttggttg gaatatccgc tcacttcacg
     3781 gtaatgtcct ttttcaaaac cattttttcg accagttaaa agcttatgac cataagactg
     3841 atgggaaaca tcccaaacga ttttatcctt tggtgaatca aatacataat gaaacgcaat
     3901 cgttaattct actacaccta gattcggacc aacgtgccca cctacatcgc tgtccttttg
     3961 caagattttt tggcgcatct cttctgccaa ctgtttcatt tcctcaacag ataatttttt
     4021 gacatctgcg gggctattga ttgcatccaa tacatgtaat actgtactca taatacattc
     4081 tctcctccgt actatcttct tttaatgaaa atagatttgt tacactttat ctattctact
     4141 acttagagtt gactctaagt caatagcatt ttaaaaaaat ttcagagaaa gcttgttata
     4201 taagtagatc ttcgcacgaa tcaggactca aataaaaacc ttctacagac aattaaaaag
     4261 ctttgtagaa ggtctcctaa ttaaggtctc atcaagaaat atgttttttt aataggtgaa
     4321 gttcctgagc aatatgatac aaatggatgt ttgcaccttt taatgagatt ggatagatct
     4381 ttgtcttttc agtttctaat ccattttgat agctagcagc atcgcacaaa accactcttt
     4441 ttttcgcaca tagacattca tcaggcaatt cttctaatga acgaaataaa ataggattta
     4501 tttcagcgat gccttcttcc gcatacaaat gaaattgggt tcgataaaaa aattgtttga
     4561 ctgaactttc tgaaacaaca aaaatttcta tttcctgtct ccccgcttgt tcactgttga
     4621 tcaaagaaag acttttgacg aattttcgaa tgaaattttc attccatctt agttggattt
     4681 gatttttctt tttccactct tccaatattt ctacaatttt tccgtacaga agttgttctt
     4741 tttctgatag aagatagatc ctttccggta aaaatacttg tccatctgcc cacaacgtac
     4801 ggacaaattg taaaaaggat tgcttgaaaa taaaattttc tgcatcgtcg atttcagcga
     4861 tactaataat ctgtaaaatc aattcattca gtgataggtc attgagaaca aaatttttat
     4921 aaacaacttc gaaatctttt cttaacagct ccatgttctc attggtataa tttctactgt
     4981 tgaacaaaga aaaaatataa tagacatctt cttcttgtaa gtctagaatt cctttttgct
     5041 cttccaatcc ttgacagact aattgaaata acggcttcgt ttctgcgctt ttcttatctt
     5101 cttctttcag tccaagggga aatgcctttc ttccgattgc gatctctatt cctcgtatga
     5161 taaaggactt ttcatcttcc gaatatcttc ttttaaaaaa atgagtctct acataatcaa
     5221 tcaactcgtt acaagcttct tctacatacg gatttttatt ttcatagcct tcccaattga
     5281 cataacgtac taaagctagc aagatgttcc gcgagtcaaa ttctgggata atgatttctt
     5341 tatttttcaa ataatcattt tctttaaaaa aatccaatac gatttttttg attgtatata
     5401 ctttgctcaa agaaataaat tcaagatcag ccagttctgt actcgtgaat ataccattaa
     5461 aataataact taaaaacttt aaaaaataag attgaccgta cagaaactgt gtgagctcag
     5521 aaagagcaat accatgtttg aatacgagtt tatacttatg cttctctctt acttgttctt
     5581 ttatacaata ttctagacac tcggctagac tttctagatc gtgaaggatc gttggtgtag
     5641 tcacccttag agaaatagct gtttgttcga tgtctatttc ttggaattca aataattgtt
     5701 cacacagata cacttttcta aatatatttc gctcgatgta attttccagc attcctgata
     5761 cacgcctcca gtttttctta tgattttatt taatcatatc tgtaaatcgt atttttgttt
     5821 cctttttatt tagaaaagcc ctttcaaaaa ccaataaaaa aagaaactgg gacaaaagtc
     5881 aaacaagctc acaatttcag cctcattgta ggtagctttg agccgtttgg acttttaccc
     5941 cagcctcttt caagaatggt tattcaaaga tgtcagcgtc tagttctttg actacatctt
     6001 tcaacaattg gataccagct gcgctgttac caaatggatc tagtgccggg ctgaagacac
     6061 ctatgccgta acgatttggc acagctgcca tcaacccgcc gcctacaccg ctttttgcag
     6121 gtacaccaac atggacggaa aactctcctg attcatcata caaaccagca gttgtcataa
     6181 ttgatttgac gattgtagca ctttcttcag taatgatttg tttgccattc caaggagcaa
     6241 ttcctttatt tgccagcaca gcagctaatt ttgctatgcc tgtagcggtg acattgaggg
     6301 agcattgtct gaaataagca tcgagtatct ccggtacgtc tccttcgatc atttgattac
     6361 ctttcatata gtaagcaaga gagcgattga tatcacctgt tcttgattca gaatgataga
     6421 tttcttcatt caatgtgata ttaggatcat cacagatctc tttcataaat gacaagattc
     6481 gctcaaattt ttcatctgca tctttaccgg caatcaacga ggttgttgca atcgctcctg
     6541 cattaacaaa aggattcatt ggatgtttgc gatgattgat ttccatattc atgatagaat
     6601 tgaaggcaaa accggttggt tccgtgttga ttgtttgaaa gactttttct attccgttgt
     6661 ctttgattgc taataacaat acgggaactt ttgacatgct ttcgatggca aagcgcacat
     6721 cggcgtctcc tccctcaatc agttgttttg tatccaaatc ataaatcgcc atacctaatt
     6781 gtttgggatc cacttcagct aatgctggaa tgtaatccgc cacttgtcct gtttgataat
     6841 agtggaaatt ttttccaatc aattgttcta attcttttac ttccatttga ataaccagct
     6901 ttcatttttt atttttctgc atgctggaat ttatgacggc atgcgtagat cgtaaatgga
     6961 ataactaaga cgataagata agaaacaatc agaatcgttt cataaatccc atgctgtcca
     7021 gctgcgatac tagcaggtgg aacaaatgag atacaaaaag caaaaagtga agtcaaaaga
     7081 cctaaacaag caacgattac ttttacaact tttccgcctg gaacctgata ggctctttca
     7141 agactgtcat ctttaaaaac cagcttcaaa tagcccgcga aaaacataaa gtacaacatc
     7201 aggtagatta caacagtcaa tgacatcgct gcaagaaatg aaagattatt ccccccgccg
     7261 cttaatgtaa agacggctgc ccagattgtt gcaacgatcc catttgcgat catcaaataa
     7321 gcaggaacac cgtgtgtgtt ttcttttgca aaatattcag gaagtaatcc ttcttctgct
     7381 gctacgtgaa gtgcttttga aggagaggtt acccaagaag caatttctcc gatgacacct
     7441 aaacaaacaa gaatcgcaag aattttcgca atccatgcaa gatttggatc aatgtgtctt
     7501 aataggaatt ctaacgattg aatcacccct gtattaagag acaaaccatg ttgaggtact
     7561 gtagtagcga cggtcaatcc gccaaaagta tctaaaatga ttgccagaat aaccaataca
     7621 aacattgcta atggataatc ctttttaggg ttttccattt catttgcatg actggcagat
     7681 gtttctacgc ccatatatgc taaaataaag gaaacaaaaa caaccagcgt aggcaatttc
     7741 gaaaaatcag gaatcaatgt tttcatagac agtatcgttt gtagtggagc gcctgaagcg
     7801 acataatgga tccctaagcc aaacaagact aatgctggca acaagatacc agctacaaaa
     7861 ccgatccgag caatcaatga agtgtatttg gttcctttta actgtgagag taaaatagcc
     7921 cagaaaataa tcaataggat tgccaatttc agatatttat tgttttgtat agcaccaata
     7981 cccgttgcat aggataatgc accagttaag aaatacagca tggtcaagta gccaactgtt
     8041 atctcaaacc attggaaaaa gattgcggca aaaccgaatc gttttcccaa tgtgcgactg
     8101 acccaagtat agacaccgcc tttttcccat cctttcaccg ttgccatctc tgctgcgcac
     8161 aatgccacag ggataaacca tagcaaaccg cctaataaca agaaaaatac taagctgaag
     8221 cctgaggtcg cgaatgtggg atattcatat aatgaaacga ccattgagat ggtcatagcc
     8281 aaaaagccaa agaacgttaa tttttttgca ggaattttcg ttttgctttg ttgcatgaat
     8341 cttcctcttt tctatttgaa tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc
     8401 aatccttctt cttgttcttc ttttgtagta gtgatcgttg taaagtgcat cgccgatttt
     8461 aaaatcactg ttgtcagcac ataggcatac acggcagtaa tgatgacacc aatcacttga
     8521 atccctaaga agtaccagct gcttggattc gttagtaaat ggctgttaga tgcaaagatc
     8581 cctatcagaa tcgtacctgt gaatccgccc atgccatgaa caccccacac atcaagtgca
     8641 tcatcccatt gtttcttttt acgaaaatca acagcagcat gacagacgat acccgcaatg
     8701 atcccaatca gcatggcact ttttgcttct acataaccgg cacaaggcgt aatggctgcc
     8761 aatccagcaa cggatcctgt tagtacgtcg acaaaatcaa agccatttcc ttttactttt
     8821 gcaatgatca gccaagtaat catcgcaaat gcaagagcga tgaatgttga accaaatgca
     8881 ttgactgctt gttcatctgc tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac
     8941 caaagcagtc ctgtaccgat agcagctgcc ataagactgc tatgctgact tctttctcct
     9001 aaaggaatct ttctttgacc aagtgtgagg atacatgcta aagagccgaa tccagctgtc
     9061 gtgtggatga ctgctccacc agcgaaatct ctaaatccca tctgatccaa aaatccgcca
     9121 ccccatatcc agtggcaaac agggaaataa atcagcagat tccaaaaaat aacaagtata
     9181 atatagcctt ttaaattcaa tctccctgca aaagctcctg tcatcaaagg aacggtaatc
     9241 acgataaaca tcagttggta aagaaagaac attaaaaatg gaatcgttgc accatgaaac
     9301 atgttgggga agcttgtaac atgtctcatc aaaaagaaat ctgccgggtt tccaataaca
     9361 ccgccgatat cccgtccaaa ggccaatccg aatccaccaa atacccacat aatcgttgtt
     9421 atcccaacgg agagaaaaga ttgagacatg atcgttagta tattattctt ccgtgctaat
     9481 ccgccgtaaa aaaaagctag accaggagtc atcaggcaaa ccattgctgt acataaaatc
     9541 atgaatgctg tatctcctgt attgataggt aatgtattca tttttttcgc ctcctttcta
     9601 cgattacgag tataaaagaa ggtgtagttg tttttttctt ctgttttttt gaattaatac
     9661 ttgcatttat aaaagacaga caattaataa aagcttgatg tgttgt
//
LOCUS       DAYYNQ010000100         9520 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000100 DAYYNQ010000000
VERSION     DAYYNQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.100"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHBJ1_002418"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACHBJ1_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM9431331.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="ACHBJ1_002419"
     CDS             1873..4539
                     /locus_tag="ACHBJ1_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM9431332.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="ACHBJ1_002420"
     CDS             4903..5655
                     /locus_tag="ACHBJ1_002420"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM9431333.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="ACHBJ1_002421"
     CDS             complement(5677..6831)
                     /locus_tag="ACHBJ1_002421"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM9431334.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="ACHBJ1_002422"
     CDS             7016..9457
                     /locus_tag="ACHBJ1_002422"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM9431335.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3077 a         1711 c         2033 g         2699 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ctgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg
//
LOCUS       DAYYNQ010000101         9500 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000101 DAYYNQ010000000
VERSION     DAYYNQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.101"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..756
                     /locus_tag="ACHBJ1_002423"
     CDS             <1..756
                     /locus_tag="ACHBJ1_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9431336.1"
                     /translation="EKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVSLARKPK
                     TNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESD
                     YDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKY
                     IFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDR
                     VGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(838..2610)
                     /locus_tag="ACHBJ1_002424"
     CDS             complement(838..2610)
                     /locus_tag="ACHBJ1_002424"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431337.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(2613..4325)
                     /locus_tag="ACHBJ1_002425"
     CDS             complement(2613..4325)
                     /locus_tag="ACHBJ1_002425"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431338.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4440..5108
                     /locus_tag="ACHBJ1_002426"
     CDS             4440..5108
                     /locus_tag="ACHBJ1_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM9431339.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(5175..5963)
                     /locus_tag="ACHBJ1_002427"
     CDS             complement(5175..5963)
                     /locus_tag="ACHBJ1_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9431340.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6046..7518)
                     /locus_tag="ACHBJ1_002428"
     CDS             complement(6046..7518)
                     /locus_tag="ACHBJ1_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM9431341.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(7648..8841)
                     /gene="metK"
                     /locus_tag="ACHBJ1_002429"
     CDS             complement(7648..8841)
                     /gene="metK"
                     /locus_tag="ACHBJ1_002429"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM9431342.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(8839..8918)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(9107..9178)
                     /locus_tag="ACHBJ1_002430"
     tRNA            complement(9107..9178)
                     /locus_tag="ACHBJ1_002430"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9143..9145),aa:Glu,seq:ttc)
     gene            complement(9190..9263)
                     /locus_tag="ACHBJ1_002431"
     tRNA            complement(9190..9263)
                     /locus_tag="ACHBJ1_002431"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9227..9229),aa:Ile,seq:gat)
     gene            complement(9276..9346)
                     /locus_tag="ACHBJ1_002432"
     tRNA            complement(9276..9346)
                     /locus_tag="ACHBJ1_002432"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9312..9314),aa:Gly,seq:tcc)
     gene            complement(9351..9423)
                     /locus_tag="ACHBJ1_002433"
     tRNA            complement(9351..9423)
                     /locus_tag="ACHBJ1_002433"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9388..9390),aa:Phe,seq:gaa)
     gene            complement(9427..9499)
                     /locus_tag="ACHBJ1_002434"
     tRNA            complement(9427..9499)
                     /locus_tag="ACHBJ1_002434"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9464..9466),aa:Asp,seq:gtc)
BASE COUNT         3007 a         2049 c         1587 g         2857 t
ORIGIN      
        1 gaaaaaaatt tatcatataa ttatgtaata catttaaaaa cttttacagg aatgattttt
       61 aaattcgcca accgtcgata tggattaaaa tataatcccg taaatggggt ttctttagca
      121 agaaaaccta agacaaacga agaaataaaa aaagaaaaag atggttattt atcaaaagaa
      181 gaagttcaaa tgattgctag taaacaaaaa agttcacatc aacagtcacg ttattcttta
      241 attactaaat ttttgttttt aactggacta cgttatggtg aattgatatc tttgactgaa
      301 tctgattatg atggtaataa aattacggtt tctggtactt atgattacga gttaaaaatt
      361 aaaacaacaa ctaaaaatac aggttcttat agaaccattg aattatcaag taatgctaaa
      421 gaaatcatag atcaattaat tgaagaaaac aaattaatta agaataataa agataaatac
      481 attttcatct ctaaaaatgg taacccgata tctattcaag catataatca atctttaaga
      541 gcagtatcca aagaattaaa cctagataaa aaagtttctt ctcacatgtt aaggcattcc
      601 cacatttctc tacttacaga attaggaatc ccattaaaag ccattatgga tcgtgtagga
      661 catgaagatt caaaaacaac attaaagatt tatactcata caactaaaaa tatgcaaaat
      721 caactagtag aaaaactagg aaaaatagaa atttaaattg ccccttttct gccccttata
      781 aaaacaaaca acataaaata tataaaaaga accccgttat aacgaggttc ttttaattta
      841 actttcaaat acaaattgat tatgataaag ttccgcataa aatccatttt ctgctaataa
      901 ctcgtgatga ctgccttctt caatgacttc cccatcacgc aagacaacga ttctatctgc
      961 attcaagatt gttttcagcc ggtgagcaat cacaaagctg gttcttcctt tgattgcttc
     1021 atccattgct ttttggatcc ttgcttctgt taccgtgtct acattacttg ttgcttcatc
     1081 caaaatcaat aaagatggat ttgtgatgat cgttcgagca atactgatca actgcttttg
     1141 acctgtactg aataagttat tttcttcgga tatctctgta tcataacctt tttctaacgt
     1201 catgatgaag tcatgaatat tcgcttgttt tgctgcattg atgacttctt catctgaagc
     1261 gtccggtttg ccgaatgtga tattgtcttt gatcgttccg gagaacaata cagattcttg
     1321 caaaacgatt cctacatgtc tacgtaatgc atcaagatca taatctcgga tatctattcc
     1381 atcgatgact actgaaccat cattgacatc gtaaaaacgg tttagcagat tcatgatcgt
     1441 cgttttcccc gatcctgttg gaccaacaag agcaaccatt tctcctttgt ccacattgat
     1501 cgagacatct ttcaagacta gtttttctgg agaataacca aaatctacgt gctgcaacgc
     1561 aagacctttt tcaacaccat caaatgcttt acagttttct ggtttgactt catctttttc
     1621 atcaaatatt tcattcaatc gtcttgcccc tgtaatcgct aattggatca tgctgtatcc
     1681 agatgaaatc tgcatcagtg gttggtaata ttgttgtgaa tactggacga atgtcacaac
     1741 taagcctaat gccaccgaac gttccaaatc accatttaat gctaaccagc cgccgaaaaa
     1801 gataactatc gctgtattga ccagtgacat tccttgcatc atcggaaata gtagaccaga
     1861 ataagcttgt cctttgaagg ttgcttcgcg aacactttga ttatgctcta agaatccttc
     1921 aatcgtttct tcttgtaagc catttgtgat gatgacacgt tgtccgctga ttttttcatc
     1981 catataacca tttagtttcc ccacttcatc ttgctgaaga tccacatatt tccgtgcttt
     2041 gcggataatc aagacagcta tcaaaacggc tactggagtg gaagcaattg tagcccaagc
     2101 taactctaca ttttggcgga acatcatgat caagatcccg acaagtaaaa cagcattcgt
     2161 gatgacttga agcaaagctt ggttcaaact attttgaata ttatctaagt cactggtaaa
     2221 acgacttaaa atttcaccgt cttgatgaga atcaaagaaa cggatcgtca atttttccaa
     2281 tttgttgaat agaccgatac gcattcggtt cgttgacttc cctactactt gtgtgaacaa
     2341 aatactatag ataaagttgg ctgcactagc tagtacataa aacagcaaga gtttccatat
     2401 tacagaaaca aatttgcttt tatcgtctac gccttgcatc aagcctccta cgtaattagc
     2461 taattcttga aaagcttccc cgatgtattg aggtgccttt acttgtagat aggtagcaat
     2521 gatcactgtg ataaagataa agaaaaagga gagtttgtac cgcttcaaat agtgatagaa
     2581 aaatttactt gcttttatta gatcagtcat acttactcct ccgttccttt ttgagtttca
     2641 aagatttcct gatagacacg gtttccagct accaattcct catgggttcc ttcgccgact
     2701 aatctgcctt catccaatac taagatgcgg tctgctttga caacagaagc aattttttgt
     2761 gcgatgacaa tcgtcgttgt tcccttcaaa tctcgatcga gtgcttctcg gaccagcttt
     2821 tcagagcgag catccagtgc actcgtactg tcatctagga tcaagatctt tggattaccg
     2881 ataacgccac gagtaatgga taaacgttgc ttttgaccac cagaaaagtt actgctacgt
     2941 tcttctattg gtgcttcgta acgatcagat aacttttcga tgaattcttt tgcttgagca
     3001 atagaagaag catgttccat ctctttttct gttgcattct ttttcccttg gcgcaagttt
     3061 tgagcaatcg ttcctgagaa taaaattgct ttttgcaaaa caaaggaaac cgtttgtctt
     3121 aaactctttt catttacttg acgaagatct acaccaccta cttcaacttt gccttctgtc
     3181 ggatcaaata gacgagggat caattgtgct agtgttgact tcccggcacc agtcgccccg
     3241 acgataccaa ccatttctcc aggtttgata gaaaagctga tatctttaag cgtatctgta
     3301 tcctcaccag gataacgaaa agaaacatgg tcaaaagtga cagaacccgt caactcttgt
     3361 tctggaacgt ctaaataagt aagatctggt tcagcatcta aaatttccgc aatccgcttc
     3421 agcgatacgg ctgctcttga agtcatcatc atcatcattc cgccaataat gattgccatc
     3481 ataatctgca tcaagtagtt catgaatgaa gcaattccac caattaacgt aggatcatct
     3541 ttaacaagat cgctcacaaa aaagatcgcc ccaaccacag ccaaattggc agccagcatg
     3601 aaggacggga tcatcacaga aaataatgta ccgactatga tgttgtgttt tgttaagtcc
     3661 tcacttactt tgctgaatga cttcaattgg ttttcttctt gtacaaaaga tttcactaca
     3721 cgaatcccta gtaagttttc ttttgcaatg ctattcacac gatcaatcag tttttgaatg
     3781 atcatgaagt gcttgcccat ccttgtaaat gaaagtgccg taatcagaaa taccgtaacg
     3841 ataagcgcta cgatgatcca ccataattgc ggcatcgtga tcatagctaa aatgaaggca
     3901 ccaataaata agaatggaat tcgaaaaagt gattgcaaag aaatcatcac taaattttgg
     3961 atttgcgtga tatcatttgt tagacgaaca acaagatttc cagcagagaa ctgttcgata
     4021 tttccaaatg aaaatgtctg gatctttcga aatgcttctt ctcggatatc tgcacttacg
     4081 ccttgagcta cttttgcaga gaaaatcgtg ttcgttacac ctgcggctaa tcctagcaaa
     4141 gccagactga tcaggtaaat accaattgtt ttcatttcat cattatcttc tgtaataata
     4201 gcttccaata cttgctgtag taacttaggt tgccataatg ctgctgcaac catgactgct
     4261 acggacagca aggaaataaa aacagctact ttatattttt tcgcatgtcg taagattaat
     4321 ttcattcgtt gattacaccc ctttgtttaa attgacagga ctaatttaac actgtaaaat
     4381 tagaccgact aacaatttac catgtttaaa aagataatgc aatacacgag gttgataaaa
     4441 tggaaataaa gctttctcaa gcaatcatca ttcaggccgc ctttgatatc ctggctgaaa
     4501 cacaagctct ttcgcagctt tccatgcgaa acatcgcaaa cagaattcat gttcaagcac
     4561 ctgctttata ttggtatttc aaaaacaaac aacagctctt gcaaataatg gctgaaacaa
     4621 tggaagaaga attagcgctt ccagatccaa ctttgccttg gaatgaacag ttgctgcagt
     4681 atatggaaaa ttactatgac ctttatacac gtttcccttg tggggcagaa ttagaaatca
     4741 atactgtgcc cgcttttccg agtcgattag aacatttgga gcaaatgatc caattacttg
     4801 ttcgtgaagg attttcagtc acccaaagtc atcaggccat tgcctctctc cacaatttat
     4861 tgattggaca gttaatggat cagcagcatg aacagcagct tcgacaaaaa attatggacg
     4921 gaaatgaatt tttgaaagaa acagttctat ttatgcgtaa ttatgtagtt gaaaatcaat
     4981 tagagggatt taaagaaagt atgcagtccc atgcagaatt gaatgaaaaa gcactctttc
     5041 taacgagtgc acaaacctat attgaaggct tatgccaaca aaataaaaga agagaaaacg
     5101 agcagtaaaa aagctcctat agagatcact atgtttgtga tctctatagg agctatcttt
     5161 ttaagactct ttttttattt ttcttccatt agccacgcca atgcacgttg aatccattgg
     5221 tcccagaaga tccagttatg atcgccaggt ccttgttcaa aggtgaccgc caagccttgt
     5281 tcctgcatag cagataccat ttcctgattt gcctgataaa gaaaatcaga ttccccacaa
     5341 gccatataaa atcttggaac tggttttccg ctttttacta gttctttcaa taagtattgc
     5401 ggatcatttt ttgaaccttc gatttgatcc aatggtccaa agatcccttc ccaaaaagat
     5461 gctttacgaa ccatcaaaag atcatccatt cgatttccta cagaaacagc gcctgaaaga
     5521 gagattactg ctgcgaaatt ttctgatttt gcaaggccta gttttaacgc gccatatccg
     5581 cccatcgata atccagcggc atatgttttt tctctttttc ttgttaattg cgggaataat
     5641 tcatgacaaa tctcaggtag ctcttctgca atgaatgtcc aatacttcat gtcataaact
     5701 gtatctgtgt accagccaag atcagtagat ggcatgatga cagctaatcc gtagtttgct
     5761 acataacgtt caatcgacgt acgtctttcc catacactat gattgccacc cattccatga
     5821 agtaaatata aaacaggtat atccgttgtc tcttgctctg aactggtgcc aatcaccttt
     5881 tttgttgttt gcggtagtat cacatttaga ttgacttcca tttctaacac attcgaataa
     5941 atatttgctt gaataaatgc catattttcg ccttccttag taagaagtta tttttgaata
     6001 actttaaatt tgaatgattc ggctacgtac atatcttaac cgaaactact gttttatttt
     6061 accacttttt tcatgaacga aaagcgttaa gaccaaagca atgatcacga gtaacaagcc
     6121 aactagataa acggaatgta acccatcata aagaattttt cttaatggta acaaaatatc
     6181 cgcatcaata tatttagccg tttgtggatt caccagttta ttcatgatat ctgcatcatt
     6241 tgttaaattt gctgctgcaa gttctcgttt agttacccca tttatcaata aaccaaaaat
     6301 agaaaccatg actgtttgac caattgttcg aactaacgta ttaaaagaag ttgctacacc
     6361 aagctggtca cgagatacac tattttgtgc actgacagtc gaggtagtca tggtcacacc
     6421 taagcctata ccgatgaccg ttgatatagc aaaaaaccat aaatagttac ttctgaaagg
     6481 cataagatac aaccaagcgc ccccaatagc tgcgattagt agtccaattc tcaagaccca
     6541 atgagttttc atttgagtca tccacttgct tgcaaggaaa gaaccgacca tccagactag
     6601 tgataatggt gctaatacca gcccaccaat tgctgcattc agccctaata caccttgcat
     6661 ccacataggg atataaacat cgattcccat caaaaaacca ctagccaatg ctgcagccaa
     6721 gttgaccaca acaaatagtg gatttttgaa taaatgcatc atgatgacag gatctttagc
     6781 tcttttttcg gcaaaaacaa aaatacctaa cattacgata ctacctagca gcaaagcaga
     6841 aacaagcgga ctcattcctt cgtctcctaa taattggaac cctaacagca atgagacaag
     6901 catggccatc aaactaaaac taccaaaaat atccattggt ttagactcat gtatgatttt
     6961 tggttcattt aaatatagcc aaatcaaaat aattaataga attccgatcg gcacattgat
     7021 aaaaaagatc cagtgccagc tgatcgtttc aacgatgaat cctcccgcaa gcggtccaaa
     7081 aacactggca atcccccagg ctgaactatt caaacctaaa acttttgctc gcttttcaat
     7141 agaataaaga tcgccgatga tcgtcaaagc tactggcatc attgccccag cacccatccc
     7201 ttgaatcgca cgagcagcaa tcaacccaat catgctttga gataaaccgc aaaataacga
     7261 accgataata aacaataatg tcccaaaaat aaagattggt ttccgtccca ctttatccgc
     7321 aagttttccg taaatcggag taagcatagc gtttgtcagc aaataaatcg aaaataccca
     7381 gttcatgatt tccattccat gtaatgaacc gacaatcgtt ggcatagcgg ttgttacgat
     7441 tgtaccttca atagccgtca tgaacgttgc aatgaaaata ctcactgtga ccaaagtaac
     7501 atttgtttct tttttcatct tattcctccc tttcattatg acaggatcta aaaatagata
     7561 aataattact atctttttca agaggttaac tgccataaaa aaagctggaa caaccaccac
     7621 ttggcgatcg ttccagctga atataactta ttttgctaaa cttgctttca acgcatctac
     7681 tttgtccgta tgttcccaag gcaaatctac atctgtacga ccaaaatgac cgtaagcagc
     7741 tgtttgtttg taaatcggac gacgcaaatc aagcatctcg atgattcctg ctggccgaag
     7801 atcgaagttt tctctcactg cagcaatcag ttcttcttcc ggaaccgttc cagtaccgaa
     7861 agtattgatt gagatcgaaa caggttgagc aacaccaatc gcataagcta gctgtacttc
     7921 tgctttgcga gcaagccctg ctgcaacaat gtttttagca atataacgtg cagcatagct
     7981 ggcagaacgg tcaacttttg tcgcatcttt accagaaaaa gcaccgccac catgacgagc
     8041 ataaccgcca tacgtatcaa caatgatttt tcttcctgtt aatccggcat ccccttgagg
     8101 accaccaatg acaaatcggc cagtcggatt gatataatat ttcgtttgat catctaataa
     8161 ttcagctgga ataacttcct tcactacttt ttcgatgaca tcatgacgaa ttgtttcatt
     8221 atctactgca tcatcatgct gtgttgaaat aacgattgta tctacgcgtt ccggttgtcc
     8281 ttgatcatca tattcaaccg ttacttgaga ttttgcatct ggacgtaaat acgttaattc
     8341 atttgattta cgcaaatctg ccaagcgtcg taccagacga tgactcaaag cgattggtaa
     8401 aggcatcaat tctggtgttt catctacagc aaacccaaac attaaacctt ggtcaccagc
     8461 accaatctca tctaatacgt cttttttatc ttcatcccgg atctcaagtg cttcatcaac
     8521 tccttgagca atatcaggag attgttcatc aatcgcaacc aatacagctg ctgtatctcc
     8581 atcaaatccg aattttgcac gtgtataacc aatttctttt attgtttccc gtacgacttt
     8641 ttgaatatct acatatgccg tcgtagatat ttctccaaat actaaaacaa gaccagttgt
     8701 tacagacgtt tcacacgcta ctcgtgctgt tggatcttgt tttaaaattg catccaagat
     8761 tgcatcactg atttggtcag caattttatc tggatgtcct tcagaaacag attctgatgt
     8821 aaataagtgt ctttctacca ttataatgat tccccctagt taaattcggt tacaaggcat
     8881 ctccgtcctg cggatccttt cgggaacttg caacgaacca agtatagcag ataaaacagt
     8941 tttctgctcg tattttttca tttttttgct taaaaaatat aaaaatattt tacattagaa
     9001 ctgtccaaaa aagcttagca caatcatcca taaagataac tctgctaagc tttttaaaat
     9061 cattctttca gataaaaaga aaaagatact caaaaagtat cttccatgac ccgtacggga
     9121 ttcgaacccg tgttaccgcc gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccaa
     9181 taaaataaat gggcctaaat ggactcgaac catcgacctc acgcttatca ggcgtgcgct
     9241 ctaaccagct gagctatagg cccataaaaa ctaaaagcgg gtgacgagaa tcgaactcgc
     9301 gacaacagct tggaaggctg tggttttacc actaaactac acccgcataa tggcgcggga
     9361 cagaatcgaa ctgccgacac atggagcttc aatccattgc tctaccaact gagctaccga
     9421 gccaaacggt tccgacggga atcgaacccg cgatctcctg cgtgacaggc aggcatgtta
     9481 acccctacac cacggaacca
//
LOCUS       DAYYNQ010000102         9474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000102 DAYYNQ010000000
VERSION     DAYYNQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.102"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..905
                     /gene="gnd"
                     /locus_tag="ACHBJ1_002435"
     CDS             3..905
                     /gene="gnd"
                     /locus_tag="ACHBJ1_002435"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM9431343.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            907..1908
                     /locus_tag="ACHBJ1_002436"
     CDS             907..1908
                     /locus_tag="ACHBJ1_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM9431344.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1905..2189
                     /locus_tag="ACHBJ1_002437"
     CDS             1905..2189
                     /locus_tag="ACHBJ1_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM9431345.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2222..3019
                     /locus_tag="ACHBJ1_002438"
     CDS             2222..3019
                     /locus_tag="ACHBJ1_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9431346.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3033..3866
                     /locus_tag="ACHBJ1_002439"
     CDS             3033..3866
                     /locus_tag="ACHBJ1_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9431347.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3884..4741
                     /locus_tag="ACHBJ1_002440"
     CDS             3884..4741
                     /locus_tag="ACHBJ1_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9431348.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4763..5272
                     /locus_tag="ACHBJ1_002441"
     CDS             4763..5272
                     /locus_tag="ACHBJ1_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM9431349.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5309..5710
                     /locus_tag="ACHBJ1_002442"
     CDS             5309..5710
                     /locus_tag="ACHBJ1_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431350.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5822..6175
                     /locus_tag="ACHBJ1_002443"
     CDS             5822..6175
                     /locus_tag="ACHBJ1_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431351.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6209..6652
                     /locus_tag="ACHBJ1_002444"
     CDS             6209..6652
                     /locus_tag="ACHBJ1_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM9431352.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6669..7754
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_002445"
     CDS             6669..7754
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_002445"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9431353.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7747..8460
                     /locus_tag="ACHBJ1_002446"
     CDS             7747..8460
                     /locus_tag="ACHBJ1_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9431354.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8667..8816)
                     /locus_tag="ACHBJ1_002447"
     CDS             complement(8667..8816)
                     /locus_tag="ACHBJ1_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431355.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9212..9412
                     /locus_tag="ACHBJ1_002448"
     CDS             9212..9412
                     /locus_tag="ACHBJ1_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM9431356.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3180 a         1494 c         2102 g         2698 t
ORIGIN      
        1 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
       61 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
      121 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
      181 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
      241 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
      301 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
      361 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
      421 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
      481 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
      541 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
      601 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
      661 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
      721 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
      781 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
      841 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
      901 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
      961 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
     1021 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
     1081 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
     1141 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
     1201 gtatctatca tacctgcttt aattgaacga aatgctgtag cagaaaataa tataactaat
     1261 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
     1321 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
     1381 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
     1441 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
     1501 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
     1561 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
     1621 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
     1681 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
     1741 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
     1801 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
     1861 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
     1921 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
     1981 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
     2041 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
     2101 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
     2161 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
     2221 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
     2281 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
     2341 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
     2401 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
     2461 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
     2521 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
     2581 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
     2641 tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat
     2701 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
     2761 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
     2821 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
     2881 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
     2941 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
     3001 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
     3061 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
     3121 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
     3181 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
     3241 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
     3301 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
     3361 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
     3421 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
     3481 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
     3541 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
     3601 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
     3661 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
     3721 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
     3781 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
     3841 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
     3901 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
     3961 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
     4021 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
     4081 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
     4141 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
     4201 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
     4261 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
     4321 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
     4381 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
     4441 aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
     4501 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
     4561 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
     4621 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
     4681 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
     4741 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
     4801 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
     4861 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
     4921 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
     4981 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
     5041 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
     5101 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
     5161 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
     5221 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
     5281 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
     5341 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
     5401 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
     5461 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
     5521 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
     5581 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
     5641 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
     5701 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
     5761 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
     5821 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
     5881 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
     5941 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
     6001 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
     6061 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
     6121 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
     6181 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
     6241 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
     6301 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
     6361 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
     6421 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
     6481 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
     6541 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
     6601 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
     6661 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
     6721 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
     6781 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
     6841 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
     6901 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
     6961 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
     7021 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
     7081 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
     7141 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
     7201 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
     7261 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
     7321 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
     7381 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
     7441 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
     7501 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
     7561 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
     7621 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
     7681 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
     7741 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
     7801 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
     7861 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
     7921 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
     7981 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
     8041 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
     8101 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
     8161 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
     8221 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
     8281 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
     8341 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
     8401 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
     8461 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
     8521 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
     8581 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
     8641 atcgtccata tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt
     8701 tcgatcgtaa cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga
     8761 ttcaaatctg tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc
     8821 attctccttt ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat
     8881 ttataagtat accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt
     8941 gcagataaaa gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt
     9001 ttcttcgatt cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt
     9061 gttattagaa aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg
     9121 atatatctac atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg
     9181 tacattgata cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa
     9241 cgcagacaaa ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt
     9301 ttcagctatc caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga
     9361 tgttgaagat ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt
     9421 gacttttaac cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DAYYNQ010000103         9452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000103 DAYYNQ010000000
VERSION     DAYYNQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.103"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(183..851)
                     /locus_tag="ACHBJ1_002449"
     CDS             complement(183..851)
                     /locus_tag="ACHBJ1_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9431357.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            985..1272
                     /locus_tag="ACHBJ1_002450"
     CDS             985..1272
                     /locus_tag="ACHBJ1_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431358.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1688..3226
                     /locus_tag="ACHBJ1_002451"
     CDS             1688..3226
                     /locus_tag="ACHBJ1_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM9431359.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3223..4188
                     /locus_tag="ACHBJ1_002452"
     CDS             3223..4188
                     /locus_tag="ACHBJ1_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431360.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4175..5497
                     /locus_tag="ACHBJ1_002453"
     CDS             4175..5497
                     /locus_tag="ACHBJ1_002453"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM9431361.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5560..5664
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5738..6481
                     /locus_tag="ACHBJ1_002454"
     CDS             5738..6481
                     /locus_tag="ACHBJ1_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431362.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6496..7488
                     /locus_tag="ACHBJ1_002455"
     CDS             6496..7488
                     /locus_tag="ACHBJ1_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9431363.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7500..8237
                     /locus_tag="ACHBJ1_002456"
     CDS             7500..8237
                     /locus_tag="ACHBJ1_002456"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431364.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8227..8949
                     /locus_tag="ACHBJ1_002457"
     CDS             8227..8949
                     /locus_tag="ACHBJ1_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM9431365.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9033..>9452)
                     /locus_tag="ACHBJ1_002458"
     CDS             complement(9033..>9452)
                     /locus_tag="ACHBJ1_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM9431366.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3109 a         1619 c         1982 g         2742 t
ORIGIN      
        1 tttaagcgaa ttttttctcc caataagtaa gaaaataatg gcaaaataaa aaaacaaact
       61 attttcgcag atcgtttgca aaaatagttt atttttttat aaagaagatt ttactgaata
      121 atatcatttt agtttacata atgatatttc tgtaaattaa ataaagtagt gaaaaataag
      181 aatcagtgct ctaactgttc aagaggatca agtggttcaa atgtttctgt agtctgtttt
      241 gcttccaatt ggggcaaaac agatccaatt tccagatact tcactttatt caatgtataa
      301 gatagaatag aacagctaag taatgtatac agaaaatgtg tgagatccaa ataaagcaag
      361 gagacaacta aaataccaaa agtaatcaag aaaagacttt tgccaatctt aatatgaaaa
      421 tatttttcaa tggcttgata aatgatatct gttccgcctg tgcttccgcc gaatcgaaag
      481 acgagaccgc aaccaatgcc agaaagaata cctgtcatca aactattgat cagtagacta
      541 tctacagcaa aatgtatatg aaggctctcc cataaatgta aaaagaaaac tagcgcaaat
      601 gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat
      661 aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga atccaaaaag attatgcaaa
      721 agcaaagcaa taccagtaac cccaccatct gccagcttag atggaatatt gattcctgtc
      781 acagctaatc cgtagattcc tgcgcctaaa atgattccgc acaattctgt aaataaattt
      841 aattttttca tttctagcct cttttctttt gaaaattttc agcaacttct ttattatact
      901 gacttatact cttacttcaa gaggaaaatg aaaaaaaatt atttttggct gtttcttttt
      961 atttatcaaa taaggagtgg aaaaatgcga gcagcagact tattgacaat tcttacaaaa
     1021 tcacccaaaa aaacagagta tcaaggagtt tttttggctc aagaacaaaa actggtgcct
     1081 gtgacaatgg tccagataaa taaagcaaat caatttatct ttttatttga acacggcaaa
     1141 gcaccacttg ctatgaaaga catctttctt tttttgatga agaaccgtga gaaagctgtt
     1201 tattatcagt caaaagaaga actcattcct ctttatggag tgaaagagat agaaaataaa
     1261 gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg atgtcaaaac aaccattttt
     1321 tacatttttg gcaaaaaagt gtcatgatac agtataatat tcgttgaaca ggccaataaa
     1381 ggtgttctcc cccacgagat ttctacttta ttggctgttt ctttttatta aaataaaagt
     1441 tgtgtgtgta aaaaagatat agctgattcc aagtgacaat ttcgtataga tttttatggt
     1501 attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac tttgttgctt ggattctttt
     1561 tttgtcagca tatttagagg tatttttttc ttttttttgt tcgtgctgta aggttaaggc
     1621 aagacaagtt agttgggaaa aaggaaagaa atgtttatac ttaaagaata acagaaagga
     1681 aggtcgcatg aataatttta tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc
     1741 tgcatttcaa catctgtcca tttctctcat ttctttgctg attgcagcac tgattgctat
     1801 tcctttagcg atttgggcag ccaatcataa aaaaatggca gaagtcttat tgcagatcac
     1861 gggtgtgctc caaacgattc cttcacttgc cttattaggt ttacttatcc catttgtagg
     1921 gatcggaact gttccagcat tgattgcact tgttatttac gctttgcttc cgattttcca
     1981 aaacacgtat attggtctag ctgaaattga cccgtctatt gaagaagcag cggtagcatt
     2041 tggtatgtcc agaatgagac gcttgctgaa agtagagctt cccatcgcat tacctgtcat
     2101 tatctcaggt atccgaactg ccttagtttt aatcatcggt acggctactt tggcagcatt
     2161 gatcggagca ggtggattag ggacatttat tttgttaggt attgaccgaa atacacctgt
     2221 tttgacattg attggtgcga tcagttctgc cttgcttgca attatattca gcggactgat
     2281 tcgctggttg cagcataaga aacctcgata ttccatcatt actttagcag cagtggtgtg
     2341 tttatttggc ggcgtttctt tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat
     2401 tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa atgtataaag aattgattga
     2461 agacaacaca aaaacagaag ttgaattaaa accgaatttt ggcaagacga gttttttgtt
     2521 caatgcactg gatcaaggtc aaatcgatat ctatcctgag ttcacaggta ctgtcctaga
     2581 aagcttggtg aaggtacctg aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata
     2641 tgacgaagca gaagaattat tgaataagca attcgatatg acattattaa aaccaatggc
     2701 ttaccaaaat acgtatgcct tggcagtcaa gagatctttt gcgaacgaac atcaacttaa
     2761 aaagatttcc gatttggcag ctatctctga tcagatcaaa gcgggattca cactggaatt
     2821 tattgatcgt tcagatggat ataaaggaat ccaagaagca tatggcttga atttttcaag
     2881 tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc aataatggag atgtcaatat
     2941 tgtggatgcc tattccacag atagccagct caaggagtac gatctagtga ctctagaaga
     3001 tgatcgaggg ttattcccag cttatcaagg cgcaccgctg atgaaaaaag attttgctaa
     3061 agaacatcct gaaatcgtta aagcactgaa taaattaagc ggaaaaataa cagaggatca
     3121 aatgattgat atgaattacc aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg
     3181 aatcttcctg aaaaatgaag gattagtaaa ggaggaagat cagtgactaa attaatcgag
     3241 ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca tcgatgacct aaatctggca
     3301 atcaaaaaag gagaaatctt tgttttggtt ggtccttccg gtagtggaaa aacaactact
     3361 ttgaaaatga tcaatggatt atctaaacca tcagcaggag acatctactt caaaggaaaa
     3421 tcgttaaatg aatataatct gcagaaaatg cggtggaata tgggatatgt gctgcagcaa
     3481 atcgctttgt tcccgaccat gacagtcaag caaaatattg aagtcatccc tgaaatgtta
     3541 ggttgggaaa agcagaaaag agcagaccgt gtagatgaac ttcttcaaaa agttggctta
     3601 tctcctgata tttatcgaga tcgtatgcct agagagttgt ctggaggaga acagcaacgg
     3661 atcgggatca tccgagctat tgctgcaagc ccagatgtga ttttgatgga cgagccattt
     3721 agcgcattag atccgatttc aagaaactca ttacaagaat tggttttgtc cttacacgaa
     3781 gaattgggca cgaccatcgt tttcgttacg cacaatatgg aagaagcaat caagctaggt
     3841 gatcgtattg catttatgaa ggatggagaa attatccaat gtgacacacc agagcagtta
     3901 ttgatgaatc ctaaaaacga ctacgttcgg catttctttg atgaaccgaa acagacgaaa
     3961 gagtggcgag tagaagattt agtcgtcaat ggatatttct taaatgaaat accagaaaat
     4021 gctcgtcaac aagtttgctt tgatacgcct atgaaagaag tatattcgct tctttcggtg
     4081 tatccaagca ttgtgatagt agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa
     4141 atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa acatatgatg caatcatcat
     4201 cggttcaggt gtgagcggct taagtgctgc ttatggattg aaagaagcag gtaaaacagt
     4261 actagtggtc gaagaagatc tatggggcgg gacttgtcct aaccgtggat gtgatcctaa
     4321 aaaagtcttg ttaagtgcag tagaggcacg taatcgggta aaacaattga gtggaaaagg
     4381 ttttaacgag atcccaactg ccaactggga agaacttcaa aaattcaaac gaacatttac
     4441 ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa gctgaaatcg atcatctttc
     4501 aggaaccgct cgttttttgg atgattcatc tattgaagtc aacgaagaag tcttccatgc
     4561 ggattactta gttttggcaa ccgggcaaag acctacgatc ttaccagtgg agggaaagga
     4621 atacttgaaa actagtgcag actttttatc attgcctgta ctgcctaaag aaatcatttt
     4681 tatcggtggt ggttatattg cttttgagct agctacgatt gccaatgctg cagggagcaa
     4741 agtaaccatt gtccatcata atcaacggcc attaaaagaa tttgaggcgt ctttggtaga
     4801 ggaagccgtc catcaaatgg aagcatctgg cattcagttt gcttttggtg ttgaaaccca
     4861 aaagatcatt tcagaaggta ctcgctatcg tttagtcgga aaagagacgg aattagtagc
     4921 agatatgatt ttttgtgcga ctgggagaca gccgaataca gaatctttag cacttgagca
     4981 agcgaatatc gtctttgata aacacggaat tgcagtcaat gattatctgc aaacgagcaa
     5041 tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa accccaaaat tgacaccagt
     5101 cgccactttt gaaggaaatt atgtagctaa gcggataacg gatgcaacaa gcgaacctat
     5161 caaatatccg attataccta cgatcgtcta cgccagtcct aaacttgctg aagtaggagt
     5221 tacaaaaagt cacgcctctt cgtcagatca agtggtagag atggatctta ccagttggtt
     5281 cacctatcat cgtgtcaatg aaccagttgc taaggctgaa ttgacctttg accagcaaaa
     5341 ttatttgatt ggtgctgcag tgattagcga gcaagcagac gaattgatcg atgatttgac
     5401 actcgtcatc aatcaaaaat tgactaaaaa agagttggat agctatatca tggggtatcc
     5461 gacattggca agtgatctct cctatttatt gaaataatca gcatgataac taaagcgctt
     5521 gttgcttttt ccttcttgtg ctacacttta aaaaatgaat acctgtttct ggggatgtcg
     5581 tagacgacat gagagtaagt tgtgaactta gacccattga acctgtcagt taatactggt
     5641 gaagggagaa acatagcaga cgcttgaagc gggttactat gtcgtgccca tttcaagcgt
     5701 tttttgttat tcatattaga agaaaggagg taacaaaatg aagaaaaatc tttatccagc
     5761 agtcagtatg gcattcattt taattatttg gcagattgtt gtcatagttt ggaaaatacc
     5821 tgcctttatt ttacccggac ctgctgctgt attttctgca ttagcggcag atgcacaagt
     5881 attactttcc cattctgtca cgacattaaa tgaagcagtg cttggtttgg taatagctag
     5941 cgttctttct tttattactg ccttggcaat ggattattgg aagtttttag aaatgtcttt
     6001 ttatccgtta ctagtgattt cacaaatgtt gccaatcatg gtgttagggc cattacttac
     6061 cctatggttt ggtttcggta tgcttccaaa agtgatcctt gttgtcttga taagctattt
     6121 tccgatcgta gtttcttttt cggatacatt gaggaaagta tcaaaagagc aaatcatctt
     6181 tttgaaaacg atgggggcag atacagggaa gatttatcga atatataaaa taccagcggg
     6241 tttatccggc ttcttttccg gtttaaaagt ggctgctact tattgtgtca gcggagcagt
     6301 cgttggagaa tggctaagcg ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa
     6361 cagctaccag atcgataaag tttttgctgc gatcatttgt gtcattctat tgagtttatt
     6421 gctgaatggc gcttgttcat tattgaaaaa agggtattat cgtatgcttt atatttttta
     6481 agagaagggg atcaaatgaa aaaattatgg cttttattac cgctactgtt gcttttatcc
     6541 gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg
     6601 acacttgttt tggattatgt accgaatact aatcatactg gcatctacct agcaaaagaa
     6661 aaaggctatt ataaagaggc tggtttaaat gtccaaatca tcgaaccagg agataattcg
     6721 acaagtattg gtctagtagg tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat
     6781 gtcacttatg ctcacgcaga tggacagaat ataccagtca aagcaattgc tacagtgatc
     6841 aaacataata cttctggatt cgccacccta tcagacagta acatccattc accaaaagat
     6901 tttgaaggaa aaacatatgc aggttggcaa agtccaagtg aagaagctgt cttaaaagct
     6961 gtgatggaaa aagacggcgg ggacttttca aaactgacaa tggtaggaag caatggagaa
     7021 gggccagaaa gcttaggaaa atcctcagat atccaatggt actttgaagg ttgggatatg
     7081 atcaaagcaa aagaagcggg tattgaacta aactatatcc cattgaaaga attagatgaa
     7141 cggttagatt actatactcc ggtaattatt acgaatgatc aactgatcaa aagtgatcca
     7201 gaattggtac agtcttttat ggatgccacg aaaaaaggct atcaggaagc tattaaggat
     7261 ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta
     7321 gaagaatcac aagcattctt gtcaaaaaat tatacggatg atcctgagaa ttggggattg
     7381 atggaagaaa aagtttggcg caattataca gcttttatgc aggaaaacgg attgatcaag
     7441 caagatgtac cttcgcaaga actattcact aatcagttta taaaatagga gtgatagata
     7501 tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca
     7561 tccatttcac tatccctaaa gataccttta catcgattgt cgcgccaagc ggagccggta
     7621 agtcaacatt gctgaaaact ttaacaggag tccagcctat gacttctgga acgatcaaag
     7681 tagacgatca aaaagtaact ggtctccata cagcatttag ttatatgccg caagaagata
     7741 tgttacttcc ttggctgagc gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc
     7801 taacaatcaa tgaagaacaa gtaagggatt atctggatat ctttgggtta aacggctatg
     7861 aacatttttt gccggaacaa ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa
     7921 caatcatgaa tccttcttct tacttgcttc ttgatgaacc atttggagcg ttagatgcta
     7981 tgaccagagg gcagatgcaa gactggatgt tgaagctgcc taaaaaagca aagcggacta
     8041 ctttacttgt gacgcatgat atcgaagaag caatttattt atcagacagg attcttgtgc
     8101 tttccgcccg tccggcacat gtgattgctg agattcaagt gcctgaaaaa cgacggtcac
     8161 gtgaatggct actgcagcaa tcagagttga agcaaacgat ttatcagtta ttggcaggtg
     8221 aattagatgt taagtgatgc tcattgtcat ttagatggga gtcagagttt agcactcctt
     8281 cagcaggaac acacgatttt gacgatcatc aattgtgatt cgccagaaga atggaaggaa
     8341 aacagacagc ttgcagcaag taagactcaa gccttaagtt acggtatcca tccttggaaa
     8401 gcagatagct atacgtttga acaagttgag ccttttttga aaaaagcaag gatcatcggt
     8461 gaaatagggt tagataatat ttggacgaat gtaccgatga ctacacaaaa aaaagtattt
     8521 gagcgacagc ttgcttttgc agccataaat gagaaacctg ttgtactgca cacaaaagga
     8581 tgcgaaaaag agatcttaaa ttatatccaa gcttacccta atcactattt gatccattgg
     8641 tattcttctt tggactatca acaagactat attgatttag gctgttattt cagcattggt
     8701 cttgatttga aaaagaatcc agcagtctgg caactagcta aagcagtccc aatcgaccac
     8761 ttacttattg aaacggatgg actacatgca gcagagtggg ctttaggggg gattttttca
     8821 gaaaaagatt atgtctcact gcttgaagaa cagttaaaga caatcgctga gatcaaagag
     8881 ctatcagttg aagaagttaa aaaaaatact tatcaaaact tggaacgttt cattagatta
     8941 ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc tctttttggc tttctttgta
     9001 taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc aaccgtttgc ttcccagaat
     9061 gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc tactctttct
     9121 tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta acgtccgatg
     9181 taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat ccattctttt
     9241 gcagagcttg tgcttgctgg agtagcagca gtttctgtta cagcaggagc aggttgtgtt
     9301 tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt cttgtacaac aggttgttca
     9361 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
     9421 tctgttggga ttgttaattg ttgtcctgag ta
//
LOCUS       DAYYNQ010000104         9322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000104 DAYYNQ010000000
VERSION     DAYYNQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.104"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHBJ1_002459"
     CDS             <1..107
                     /locus_tag="ACHBJ1_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM9431367.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHBJ1_002460"
     CDS             complement(176..904)
                     /locus_tag="ACHBJ1_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM9431368.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHBJ1_002461"
     CDS             complement(917..2197)
                     /locus_tag="ACHBJ1_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM9431369.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHBJ1_002462"
     CDS             complement(2338..2940)
                     /locus_tag="ACHBJ1_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM9431370.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHBJ1_002463"
     CDS             3185..3391
                     /locus_tag="ACHBJ1_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431371.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHBJ1_002464"
     CDS             3703..4422
                     /locus_tag="ACHBJ1_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM9431372.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHBJ1_002465"
     CDS             complement(4503..5186)
                     /locus_tag="ACHBJ1_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM9431373.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHBJ1_002466"
     CDS             5405..7591
                     /locus_tag="ACHBJ1_002466"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM9431374.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7937..8347
                     /locus_tag="ACHBJ1_002467"
     CDS             7937..8347
                     /locus_tag="ACHBJ1_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9431375.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8361..8717
                     /locus_tag="ACHBJ1_002468"
     CDS             8361..8717
                     /locus_tag="ACHBJ1_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9431376.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3364 a         1525 c         1745 g         2688 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
     7861 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
     7921 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
     7981 agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
     8041 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
     8101 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
     8161 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
     8221 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
     8281 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
     8341 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
     8401 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
     8461 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
     8521 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
     8581 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
     8641 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
     8701 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
     8761 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
     8821 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     8881 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     8941 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     9001 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     9061 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     9121 tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa aagagttgac aattacttgt
     9181 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     9241 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     9301 attcaattca agacaacaaa ca
//
LOCUS       DAYYNQ010000105         9096 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000105 DAYYNQ010000000
VERSION     DAYYNQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.106"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHBJ1_002469"
     CDS             <1..177
                     /locus_tag="ACHBJ1_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431377.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHBJ1_002470"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHBJ1_002470"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM9431378.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHBJ1_002471"
     CDS             1199..1771
                     /locus_tag="ACHBJ1_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431379.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHBJ1_002472"
     CDS             complement(2023..2280)
                     /locus_tag="ACHBJ1_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431380.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHBJ1_002473"
     CDS             complement(2406..3221)
                     /locus_tag="ACHBJ1_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431381.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHBJ1_002474"
     CDS             3738..5063
                     /locus_tag="ACHBJ1_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM9431382.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHBJ1_002475"
     CDS             5056..5406
                     /locus_tag="ACHBJ1_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431383.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="ACHBJ1_002476"
     CDS             5388..5573
                     /locus_tag="ACHBJ1_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431384.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBJ1_002477"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHBJ1_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM9431385.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHBJ1_002478"
     CDS             complement(6724..7677)
                     /locus_tag="ACHBJ1_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431386.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..>9096
                     /locus_tag="ACHBJ1_002479"
     CDS             8289..>9096
                     /locus_tag="ACHBJ1_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HFM9431387.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGR"
BASE COUNT         3108 a         1442 c         1623 g         2923 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaa
//
LOCUS       DAYYNQ010000106         8940 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000106 DAYYNQ010000000
VERSION     DAYYNQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.107"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHBJ1_002480"
     CDS             complement(13..1152)
                     /locus_tag="ACHBJ1_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM9431388.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBJ1_002481"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="ACHBJ1_002481"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM9431389.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBJ1_002482"
     CDS             complement(2965..3720)
                     /gene="tpiA"
                     /locus_tag="ACHBJ1_002482"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM9431390.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBJ1_002483"
     CDS             complement(3824..5014)
                     /gene="pgk"
                     /locus_tag="ACHBJ1_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM9431391.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBJ1_002484"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="ACHBJ1_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM9431392.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="ACHBJ1_002485"
     CDS             complement(6166..7257)
                     /locus_tag="ACHBJ1_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM9431393.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBJ1_002486"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="ACHBJ1_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM9431394.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="ACHBJ1_002487"
     tRNA            8800..8871
                     /locus_tag="ACHBJ1_002487"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
//
LOCUS       DAYYNQ010000107         8914 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000107 DAYYNQ010000000
VERSION     DAYYNQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.108"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(406..903)
                     /locus_tag="ACHBJ1_002488"
     CDS             complement(406..903)
                     /locus_tag="ACHBJ1_002488"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM9431395.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(943..1752)
                     /locus_tag="ACHBJ1_002489"
     CDS             complement(943..1752)
                     /locus_tag="ACHBJ1_002489"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431396.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2086..2943
                     /locus_tag="ACHBJ1_002490"
     CDS             2086..2943
                     /locus_tag="ACHBJ1_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9431397.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2936..3838
                     /locus_tag="ACHBJ1_002491"
     CDS             2936..3838
                     /locus_tag="ACHBJ1_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM9431398.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3871..4221
                     /locus_tag="ACHBJ1_002492"
     CDS             3871..4221
                     /locus_tag="ACHBJ1_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM9431399.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4270..5193
                     /locus_tag="ACHBJ1_002493"
     CDS             4270..5193
                     /locus_tag="ACHBJ1_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM9431400.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5271..6164
                     /locus_tag="ACHBJ1_002494"
     CDS             5271..6164
                     /locus_tag="ACHBJ1_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9431401.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6202..6765
                     /gene="amaP"
                     /locus_tag="ACHBJ1_002495"
     CDS             6202..6765
                     /gene="amaP"
                     /locus_tag="ACHBJ1_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9431402.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6779..6967
                     /locus_tag="ACHBJ1_002496"
     CDS             6779..6967
                     /locus_tag="ACHBJ1_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9431403.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6980..7882
                     /locus_tag="ACHBJ1_002497"
     CDS             6980..7882
                     /locus_tag="ACHBJ1_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9431404.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7914..8156
                     /locus_tag="ACHBJ1_002498"
     CDS             7914..8156
                     /locus_tag="ACHBJ1_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9431405.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8412..8783)
                     /locus_tag="ACHBJ1_002499"
     CDS             complement(8412..8783)
                     /locus_tag="ACHBJ1_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431406.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3107 a         1540 c         1726 g         2541 t
ORIGIN      
        1 gttcgagtcc tacaaggtac ataagaggtt gtgaaaaagc tgccctgcat caagtaataa
       61 gaacagccaa gtaaaaacag cttcttatgt ttttacttgg ctgttcttat tactgcagat
      121 gcaagctttt gaataccgtt taaggaagag gttgtgaaat cagcgttttg acctgagtat
      181 tagcttaaca aacggaaaaa tagttcctca tattttgccg tttgttaagc taataccgag
      241 ggtcagctga ttgaacacca tttaagaaag aggtcgtgag cctgccgttt cactctgtgt
      301 cataaaaaag ccaatctact ttgatagaaa aagcacgtag gactaaaaca gttctgcgtg
      361 ctttttttat tgtgtgttaa atatcgtaaa tgaagataga tatttttatg attcgacgat
      421 agagacgttg aaatctttca acagctctaa agcggattct ccgtgttttc tactggagac
      481 taacattgga tcaacaaaaa agtctgcttc aggaaaagca ctctgcaggc ctatcagatt
      541 agagagtaca cacaagtttg tttcaatccc gactacttcg atctcttttt gttcttcttt
      601 gctttcaaat aaagtttcct ggatttcagc taaagtttca ggtggaatcg taaagtaata
      661 ctttttaatc actcgttcat tttcttgtgg agaaagaaga gggatcaatt gcaaatcttc
      721 tcgttcttct tctttgtcct tcagttcaac tggtatatcc cgtgtgaaga gaatatattc
      781 attattttgg cgtgctttag ctaatctttg agcaatacgt tctgccaatt gatctgactc
      841 tggaatataa aattcgctgt ttggatcaag aatatgattt tgcatatcaa taataactaa
      901 cattttcttt cctcctacat tcatatcgtc attttattct ttttaataga agttttttaa
      961 aatataagca acaccagctt cttcattcgt cagtgttact tggtttgcaa tttcttttac
     1021 ttgtcgctgt gcattcccca tcgcaatact caacccgctg taattcaaca tagcaatgtc
     1081 gttttcttga tcgccaaaag ccatgatgtc actcgattcg attccaaggt aatccgccag
     1141 aaaaacaagt gaatgagctt tagtgatacc tgtaggcgtg atttctaaga aatgcggatc
     1201 agagaatact gattggatac catctgcttg cagatctctt tgtagcttct gtagtttttc
     1261 ttcactctta tctgttaatg ctaatttaag atagctaaga gaaggatctt ccaacatatt
     1321 taaaatttga tcaaaagaat ggttctgtaa attgaaatgt tcataataag ggtcatcttt
     1381 gactagattt gattcatcga atgtcagctg atcatcttgg gtttctcggc tagcaatgat
     1441 ggatattttc gcttcttgag catagaagaa ggcttttctc agtgtatcgc tatctatttt
     1501 tgaacgtctg agaactttcg ccatttttcc atccttcatt tcagcaatca gtgaaccatt
     1561 ggaagaaatc aaatagccac cgattcctaa ttcggaaaaa atatctttag cagaaatgct
     1621 gttccttccg gtagcgacgg ttacgattcc gccattttca tcaaatttcc gaagtgcttg
     1681 gatattttct tctggtatct ttccatttcg atcaagcaag gtaccgtcta aatcaatcgc
     1741 tgctagtttc attttatctt cctactttct ttttacatat ctattttcct gttaccttaa
     1801 gattaaatga gaatttaaaa aaatgaaact aacttgcttt gtcattatga attttttcaa
     1861 aaaacattct taagaaaaag gtggctatat atagtagttt ttggtcaata cctttgaata
     1921 aaaagccatg aaaaacgaat agttagcgct taccgatgcg ttttgtgata aaaaaatttt
     1981 aaaatattca atgaaaacaa agcatatatt ttgctttaaa accaaatgag gaatattcta
     2041 gatgtagaac aggttagaaa aagattagaa aaaggagtga ttttgatgga gaagagatac
     2101 ggcgtagttc ctgtggtaac tgcttcagac gaaaattatg ctccttactt gagcgtgatg
     2161 atcgcaactg cattagaaaa ttgcaataaa acaagacgca taaaatttta tgtgattgac
     2221 gatggtctat cagaatatag taaagaagga ttggaagaaa cggtaaataa atattcaagt
     2281 aatgcaagta tccaattcct gacagtggaa aaagatatct acgaagactt cctagtcagt
     2341 gaccatatca caactaccgc ttacttaaga atttctttac caaacttatt agctaaagag
     2401 gattataaga aagtccttta cttagattca gatgtgttag tactagacga tatcgtaaaa
     2461 ctatatgatg agccgttaaa tggaaaaaca atcggcgcta ttatcgatcc aggccaagtg
     2521 aaagctttgg aaagattagg aatcgattcc gatgacttgt acttcaattc aggagtgatg
     2581 gtgattgaca ttgatcaatg gaataaaaaa gaaattacag aaaaaactat ccattacctc
     2641 agtgaaaatg gcgatcgaat catctatcac gaccaagatg ctttgaatgc tgtactttat
     2701 gaagattggg aacaacttca tccaaaatgg aacatgcaga cttcgctgat ttttgaacgt
     2761 catccagcac caaatgaaaa atatgaacgt cagtataaag aaggaaatga aaaaccttcg
     2821 atcgttcact ttacaggaca tgataaacct tggaatacgt tgaaagatca tccgtatacc
     2881 aatttatact taaaaaaatt agcacacagt actctaacga aagtaggcga gatcaatgaa
     2941 taaaaaagaa atagcagtag tcgcaagttg taacacaaaa ttcgtaccac atttagctgc
     3001 attatttgtc tcagtattag ataattgcaa cccgtctaaa tttgttcggt tctatgtgat
     3061 tgacgatgat atcgattttg aaagtaaaca gcttttacgc ttctcagtga aaaacgcacg
     3121 aatgaactca gatgtggaat tcttgaaaat caataaagaa ttctttacaa atgtggtaat
     3181 cagcgatcgt attccagaaa cagcttatta tcgtattgcg attccggaat tgttccgcgg
     3241 cacagaagtt gaacgtatct tatatatgga ttgcgacatg atcgctttac aagatatctc
     3301 taaattatgg cgtcttgact tcggtgattc aatcgtagca gctgttgaag acgctgggtt
     3361 ccatcaacga ctggaaaaaa tgaaaatttc agctaaatca atgcgttatt tcaactcagg
     3421 tttgatgctg atcaatgtga aaaaatggtt agatgaaaat attacgcaaa aagtattaga
     3481 tttcatcgaa cataatccag aaaaattacg attccatgac caagatgcat taaatgctat
     3541 cttacatgac cgttggttac cacttcatcc gcgttggaat gcacaaggct atatcatggc
     3601 aaaagcaaaa aaacatccaa cagcagcagg tgaacgtgaa tacgaagaaa ctcgcaacaa
     3661 tccttacatt atccattttt caggacatgt gaaaccgtgg agcaaggatt ttgaagggcc
     3721 aacgaagaaa tattacgaaa aatatgcagg gatgacggct ttccgttgtg tagctaaatt
     3781 ccctaaatat ccaaaatatg caaaagttca accaaaaaca gatgtagttg ttaaataata
     3841 gaagaccaca actgaagagg gggagtagga atggaaatta caatgtctaa aaatgcagta
     3901 gagacgttaa ttgaaaaggt tggggaaaat acaaaaatcg cactcgcgct tatcaatgat
     3961 tccgatccat ttctaaggga caaaggtgct tttgcaaaag gcagcttttt tcaaatcatt
     4021 ccttttgttt cggaatttgg agaatatgcg actaaaatcg agcatccatt attggacatc
     4081 tatacatcta aattagagca aaattatttc ggtaaacgat tgaacatgga cttcaacaaa
     4141 cagcttgaca gcttttcatt agaaaatgaa atcgcagtac tcgattataa tatcaaatta
     4201 aagaactgct tcttcagcta atagtttcga gaaagtccaa aaaggaggta tacattgaga
     4261 tactactttg tggatgaaga aaagaatgta atagaaacag atgaaaaaaa atataattgg
     4321 ctcgtacttg atagtgcaaa gcccgaagag attgagcaag caatcgaaga attcgagctt
     4381 cctgatgata ttttcgtagg aaccgaatat ccggaagaag tttctcggat ggagcgcctt
     4441 tatgagacga aactagagca tccattttct ttggtagtga ttaatctaga tcagtcagat
     4501 aatcgtatcg aaaaaaaatt aacgccaata tcttttgtac tttccgataa tctcttgatt
     4561 acttgtatcg atagaaaaac cgaatttgtg gatcggctgc tgacaaagca tggaaatcat
     4621 ttaaactcgt ttgaaaagat catcacgtat acattattag atatttacac ccattacgtg
     4681 aaagaactaa gagagatgaa aaagcgaatt gacgctttgg ataaagcagc tcgtaaaaca
     4741 acggaaaatg aagaactgta taaacaggca gatcttgaaa gagacatcgt atatatcgat
     4801 cataccttaa gagatcaacg ggaaacaatg gatcaattat ggaattctga aactttcatc
     4861 aaacgtttag acgatgaaca gctactctat gatgtccatt tgagacagcg acaaacagaa
     4921 aaaatgatcg agatctatcg agatttatta gagagtattg gtgatctatt caacgggatg
     4981 atggacaata atctaaatca tatcatgaaa tatctagatt cagctgcatt aatcatttcc
     5041 gttccggcgt tgttcgcagg gatttgggga atgaatacag gaggattacc gggtaaaagt
     5101 tctgctctag gctttatact cgtagtcttt ggctctttga tagcagcagt agttttaggt
     5161 taccatttat accggaaaga ttatacaaat taaagaatct atttaaaaca ggtatcaaac
     5221 aaagaaattt gttttggcaa tacaatttaa aaactaggag gaatacagca atgacagaac
     5281 caaaattaat cgacccgcga aacttgtacc atacggagaa atttcctaaa caagatcagg
     5341 atacaccagc tttacagcag aatatgattc ctgttcctga ttgtggagaa gaatcctatg
     5401 aaggcaataa tcaactagaa aaccgccgtg tactaatcac tggtggagat tcagggatcg
     5461 gtcgtgcagc agcgattgca tttgctagag aaggggcaga tatcgctctt caattcttcc
     5521 caggagaaga aaaagatgca gaggaagtag caaaatacgt gaaagatgca ggacgtaaga
     5581 ttgtcttact accttttgat cttcgtgacg aacaagcacc taaagaaatc atagataaag
     5641 ccgtgagtga acttggcggg ctagatacga tggtactaaa cgcaggacag caaatttctt
     5701 gtcagtctat tgctgagtta ccacttgagc aagtcaaaga cacatttatg ataaacatca
     5761 tcgctatgtt tgctttagtg aaagaagctg ttccgcatat cccagcagga ggagcaatcg
     5821 taacaacaac ttctgttcaa gcattcaatc caagcgaaca tttattagat tacgcagcaa
     5881 caaaagcttc aatcgctaac tttacgatcg gtttagcaaa acagcttgca cctaaaggaa
     5941 tccgtgtaaa tggtgtagca cctggtccaa tttggacacc attacaacta gacgaaggac
     6001 aaccttctga ttcgatccct gaatttggtc agcaaagttt actagaacgt gcaggtcagc
     6061 caggagaatt agcaccagtt tatgttttct tagcatcgaa taaagcaagt tatgttacag
     6121 cccaaattta cggagtaact ggtggagaag cgatcaactt ataagaagtt gatcgtttca
     6181 cccctatgag gaggtgcgaa aatgaataaa ggtgtaaaaa cgatcggagt gatcggaaca
     6241 ttacttttgc tatctgtgct tttgtttgcg atactattaa gtgcaaccta tccattgcct
     6301 tttcagctag agaagtttcg attcttttcc atcactaatt catacgtgca acaatatgta
     6361 ttttggcttg ccgttgcatt tgctattttg acaattatca cgttacttgt tttcctgttt
     6421 tatccaaaaa caatcggaaa ctttctactg aaacatggtg atggggagtt gacgttggat
     6481 aaaaaagcaa ttgaaggaat ggttcgttct cacttgcatg aagatgaatt catccattca
     6541 ccaaaagtaa atgtcaaagc aacgaaaaac aagatcgccg tcaatgtaaa aggggagttg
     6601 aaacgaactt cagcactcat tggtaaaaca ggaacattga tgaaagaaat cgaaaatgaa
     6661 gtacaacaca tattgggaac aaaagagcca gtgaaagtat cggtgaaata cacaggcttt
     6721 gaagaaccag ctaaacgaag caatgaccgc aaacgagtag aatagggggg gaccaaaaat
     6781 gaaagaacta ttttcgacat acaagctccc tatcatcttt ggggtacttg gattgatatt
     6841 agccgtttta tggatcaccg tgggcttttt caagacctta ttgatcttga tcatggcagc
     6901 gatcggcgtc gctatcggtt tttatctgaa acaaacgaaa atcttagatc actattttaa
     6961 atcttaggag gaaacatcca tgaataacaa taacaaaaat aataacaata aaatgaacaa
     7021 ccaaaaacca gtgtctgcac atccagaccc aacgacacca aaccaatcaa atggtgtgaa
     7081 cgatgaaaag aacaaaacag caccagtatc tgcacatcca gatccagtag tccctaacgt
     7141 tcaacaagga gtagaagaga agaaacatga tgcagaaaag catgcacctc atcatccagg
     7201 aactccagaa tctaaagttc cagaacaaca tgatgcaatc gatccttcta aacctgtgga
     7261 agagcaaaaa catccttctg accatgcgaa agcacaagaa aaacttagtt ctgaccacac
     7321 aaacggacaa ggaaaacttg gttctgatca tacaaataac caaggaaagc atgattctga
     7381 acaggaagat tcaatgcgcg gtgagctgaa ttatgaagat aaagttgtgc aaaaaatcat
     7441 cggaatcgca attgaacaag tagacggttt attatctgca aacggtggtt tcttctctaa
     7501 tgtggcagga aaacttgtga atacagataa tgtaacggct gggatcgaaa cagaagtcgg
     7561 taaaaaacaa gtagcagttg atatggatgt cattgtggaa tatggcaaag atatcgaaaa
     7621 aatctttgaa gaaatgcaag aagtaatcgg caaagaagta aaaaatatga cacatttaga
     7681 agtgatcgaa gtcaacgcta acgtagtgga catcaaaaca aaagaagaat ttgaaaaaga
     7741 tcaagaaacg gtgcaagaca aagtaactga cgcagcgaaa aaaaccggtg aatttgcttc
     7801 gaatcaaaca gataaagtaa aatcagcagc tggaagcggc gcacaaaaag taaaagaaaa
     7861 tacagaacca cgggttcagt aataaggaag taaaacatta ggaggaacga aacatgggat
     7921 ttatttggtc acttatcgtc ggaggaatta tcggagcaat tgcaggagca atcacgaata
     7981 aaggttcttc gatggggatt attgcaaatg tcgtagcagg tttagttgga tcagcacttg
     8041 gtcaagcatt attaggttct tggggaccaa gtattgcagg aatggcaatc gtgccatcat
     8101 tgattggtgc catcatttta gtactaatcg tatcattctt tgctcgaaaa ctgtaaaaag
     8161 acttttaaaa agaggtcgtg aaaaagctgt tcttcatcaa gtaataagaa cagccaaaca
     8221 aaaatagctt ctcatatttt tcatttggtt gggcatatta tcgaagatgc aagcttttga
     8281 acactgttta tacggaataa gaggctgtga caagactttt gtcacagcct cttatttatg
     8341 cgggcaaatt cccaattaga tttttaaaag tttttcctta aatgtgtctt tattgataca
     8401 gagaagcttc tttaataaaa caatgcctcc ctaaattttc ttgagatagg aaaggtgaac
     8461 atacaaaata atacgaccac tttgaaaacc cagatgatcc acaaatggat cgtatgatac
     8521 tgatgaagta aaaagcaatg gatcagaaac tcaaagagat tgaaaaagac aaggaagaaa
     8581 taatatctct tttttactct tgtctgtttt agccaataat agctcgtaaa gtaatctaga
     8641 caaacaaata gaagtaaaca aagtttcgta agagcagcaa tcgtatcatg ttgctctgct
     8701 agacgattga cgagcaggaa aaatgttggt aatggaatat agatcacgag taatgaaaga
     8761 aaaaagtaaa caataaatgg catactattc ctcctaattg tgtgagttcg ccctcatttt
     8821 aaaataacga tatttgtttg tgaagctttt tcgtgtcgta acaaatgtta tctaatgtat
     8881 ttttaaataa ataatcatta ttatcaaaat aatg
//
LOCUS       DAYYNQ010000108         8710 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000108 DAYYNQ010000000
VERSION     DAYYNQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.109"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..189)
                     /locus_tag="ACHBJ1_002500"
     CDS             complement(<1..189)
                     /locus_tag="ACHBJ1_002500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431407.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVI"
     gene            complement(182..811)
                     /locus_tag="ACHBJ1_002501"
     CDS             complement(182..811)
                     /locus_tag="ACHBJ1_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9431408.1"
                     /translation="MSNRGIYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGS
                     PVIFKQQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPE
                     LWNILKGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFE
                     KDIEYVENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(804..1922)
                     /locus_tag="ACHBJ1_002502"
     CDS             complement(804..1922)
                     /locus_tag="ACHBJ1_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9431409.1"
                     /translation="MENKRILLASPHMSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEE
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPV
                     FVDSDYETWNMNPEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGT
                     DVAQTLFEKGICLPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(1924..2556)
                     /locus_tag="ACHBJ1_002503"
     CDS             complement(1924..2556)
                     /locus_tag="ACHBJ1_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9431410.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(2567..4384)
                     /locus_tag="ACHBJ1_002504"
     CDS             complement(2567..4384)
                     /locus_tag="ACHBJ1_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFM9431411.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(4397..5161)
                     /locus_tag="ACHBJ1_002505"
     CDS             complement(4397..5161)
                     /locus_tag="ACHBJ1_002505"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM9431412.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(5189..5887)
                     /locus_tag="ACHBJ1_002506"
     CDS             complement(5189..5887)
                     /locus_tag="ACHBJ1_002506"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM9431413.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(5899..6678)
                     /locus_tag="ACHBJ1_002507"
     CDS             complement(5899..6678)
                     /locus_tag="ACHBJ1_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM9431414.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(6694..7638)
                     /locus_tag="ACHBJ1_002508"
     CDS             complement(6694..7638)
                     /locus_tag="ACHBJ1_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM9431415.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7684..8463)
                     /locus_tag="ACHBJ1_002509"
     CDS             complement(7684..8463)
                     /locus_tag="ACHBJ1_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431416.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         2362 a         1796 c         1469 g         3083 t
ORIGIN      
        1 tatcacatga cgatttttaa tcatatgcct ttctttactg gatagaaatt gttttcttat
       61 atgtgagtaa tcagatacaa cattaaaata ttcgatactg ttttccgtca gaatttcctc
      121 taatatattc gttcgactga tataatcaaa aggatgaata agcagccact tttttttatt
      181 ctcactcatc tatcttcgca ccctcaaatt tctcagtagt cccacttgct actgatgtta
      241 ttccatcacg gacaataact ttctttagcg ttaaaaaaaa tatttttatg tctttcatca
      301 ttgaaatgtt ctccacatat tcaatatctt tttcaaattt ttctttccaa ctaattgcat
      361 tacgaccact gacttgagct aatccggtta aacctgggcg cacaaaatgt ctttttcgtt
      421 gttcattact gtatagtgaa agatactcaa ctaacagagg acgcggtcca ataatactca
      481 tatctccttt taaaatattc cataattctg gtagttcatc aagactagtc gaacgcaata
      541 ttttaccaaa ttgagtcaat ctgatatcgt ctgataataa attaccttct tcatctcttt
      601 gatctgtcat tgttcgaaat ttatacatca gaaaaatctt ttcatgtttt ccaggtcgtt
      661 gttgtttaaa gatcactggc gatccaagct ttattcgaac taggattgca acaaacgtca
      721 tcagtggcca taacattatt aaacctaaaa ttgaaaaaag aatatctatc aaacgtttaa
      781 agaacttttc gtagatacct ctattagaca ttttgcatca ctcgcttaat tgtattagtt
      841 actcgataca aatcatcatc ggtcatttta gaatctgaag gtaaacagat acctttttca
      901 aacaatgtct gagcaacatc tgtaccaata tactcatact tatcgaaaac tggctgtaaa
      961 tgcattggtt tccatatcgg gcgggcttct atattatttt tatctaactc atcaataaga
     1021 tgattcgtat ttattccctt tactgttatg acacttaacc aataatttgg ttcattccaa
     1081 ttattttctg gcatgaattg tatatttgac aatttagaca attctttttt ataaaaacta
     1141 tgaatatatc gttttttctc aattctttca tttaaaactt tcaattgtcc tcgaccgata
     1201 cctgcaacga tattgctcat tcgataatta tagccaattt cattgtgttg ataatggcgt
     1261 gccttctcac gagattgcgt agcccaaaac ctcacttttt ccaccttttc tttgtcatta
     1321 gacacaagca ttccgccacc tgaagtcgtg attatcttat ttccattaaa agaataaatt
     1381 ccatagtcgc cgaatgtacc agtatatttt cctttatatg ttgttcctaa tgattctgca
     1441 gcatcttcaa ttaaaacaac attatgttct ttgcatattt tagtaatttc atctagttca
     1501 gctgataaac cgtacaaatg cactactaat acagcttttg cgttaggata tttttctagc
     1561 gctgttcgca aatgttcagg gttcatattc caagtttcgt aatcactatc aacaaatact
     1621 ggaatagctc cttgatagat gattggattg gcagtagctg agaacgtcaa agactgacaa
     1681 atcactatat catctttacc gaccccagcc gcttttaaag ctagatgtat agctgctgtt
     1741 ccagaagata gtgcagctgc atgactagca ccgacatatt ctgctaattc ctcttcaaaa
     1801 ccattgacat tcatacccaa aggagccacc caatttgtat caaatgcttc ttttacatac
     1861 tcttgttcat atccttcatc actcatatga ggagatgcta ataatattcg tttattttcc
     1921 aacttaatcc catctttcta tgactttggt aggtacgcca acatttttag tcttattatt
     1981 aatatttttt attactacac ttcccgcacc tataatgttc atatttccaa ttcttagatt
     2041 ttgtattatt ttagtaccta ctccaatttc atttttatct cctaatagaa catgtcctga
     2101 aatatttaca cttggaaaaa ttgaattata attaccgatt tgagtatcat gtccgatagt
     2161 agagccgata ttgatcatat tgaaatttcc taaaactacg tcagcagtgt aagtcgtata
     2221 gggcatcaaa atattgccaa ttcctaactg aacatttatg cccactagtg cacttgatgc
     2281 aactaaatta gggaaaatca atttattatt tttatttatc ttttgatata ttttctcttt
     2341 gatatctggc gaagcgattc ccagaaaaat agcagtttct tcttctaatt ctaataacga
     2401 gtctattgtt cccaatacag tttttcccac taattcttga ttttgcattt ttaacgaatc
     2461 atcgacaaaa ccaatcaatt catatctaga gaaactttct agtagaaaag caatctcttt
     2521 gccaaatcca ccagctccta caataattgc tttcacttta ctccccctac ttattcgaag
     2581 cattcgcaaa agtgacaatg tctttagcca attgttcatc gtcttgcggc aggctcttga
     2641 caaattccat cactgtttgc atggaataac ctttgatgtt tcccacaaag atcttctcgt
     2701 aaactgcttc atcgtttctt tccttatcta acaatagttc ttcatatagt ttttcccctg
     2761 gacggatacc tgtttcaatg atttcgatct cgtcttcgct atatccgctc aatcggatca
     2821 tgttttttgc tagatctacg attttcactg gttcactcat atctaaaata aagatttcgc
     2881 cgccttttgc taacgcccca gattgaataa ctaaacgact tgcttcagga atcgtcatga
     2941 aatatcgggt catacgaaag tctgttacag tgactggccc tccttgagca atttgttccc
     3001 ggaatactgg aatcacacta ccgcgagaac caaggacatt cccaaatctg acagctgaga
     3061 atttcgtaca tccttcttca ttcaacccag tcacgatcat ttcagcgatt cgttttgttg
     3121 aacccatgac gtttggcgga ttgttggctt tatctgtcga gaccatgacg aagtttttta
     3181 ctttcgctgc tttggctgct tctgctacat tttttgtacc gaaaatattg tttttgactg
     3241 cttcttttgg gttatattcc atcaatggta catgcttatg agcggctgca tggtagacga
     3301 tatctgggtg gtattgttcc atgatctcat tgattttctt gcgatcttgg atatccgcaa
     3361 tgatcggcac gatttctgta ggacactttt gatgatgcgt gcgtaattca cggtcaatca
     3421 agtaaatcga gttctcccca tgacctaaaa gtaatagttt tgcagggtca aattggatga
     3481 tttgccggca gatttctgaa ccgattgatc ctcctgcacc ggtgacaaga atgactttgt
     3541 ttgtgatctg gtctttgatg gattcgatat ccaactctac ctcatctctt cccagtaatt
     3601 ccactacgtc gatggttttt aattttgata cgctgatttt ccctgaggca agttcttcca
     3661 tagaaggcat cgtattgact ttcacatgag cagattcgac taactcgaag attcttcgta
     3721 atttttttct tgataaagaa gggatagcga tcgttaccat ttggatgtca tatttttcga
     3781 tcaattccgg tatatctttt aatgacccca gtactttttt tcctgaaaga taggtatttc
     3841 ttttattcgg atcatcatcc acgaatccaa tgacatggat atcttgagcc gttttagaac
     3901 caagaaagct gttgtataaa atacgtccac cttcgccggc cccaacgatc aaggtatttt
     3961 tagcggaatc tgttgataga tacctcatat tttttgtttc gatatagata cgccagatca
     4021 atcggctgcc aatgatcagt aatagcgaaa gtaggtatgc aaagatcact aaacgtaaac
     4081 tgtattgttt atttatgaaa agcaatatga tgatacttga ggccgcactt gcagataacg
     4141 aaccaaaaat cgcaatgatc tctcttaaat ttgtataacg attgattcga gtaaaaactt
     4201 tgaataaact accatagaat aaataaaaac caatcgacaa accgatagag attaaaataa
     4261 ggtccattgg tatagctaca aacggcttca tgaatttagc tgctgcaata ttcgcagcaa
     4321 tcaacaacaa gctgtccaca aataccaatg tggcaatttt tcttcttctt gttaatacaa
     4381 acaccctaca ttccccctaa aataaaccaa acttcttctt ttttacctct tcatagactg
     4441 gataactgat ctcatccccg ttgatcaggt cctttgcgac ttgtttcatc tgtgctactt
     4501 tttccttacc aaagtccttg gcgatttgct cataacattc ttttaagtaa aaggtacgtt
     4561 tagagacccc atgggcatcg gaagcagcca tttgcacgag gttatgtttg accatcattt
     4621 tggctgtttt ctggatgtct tttccaaata caccgacata acttggtgcg gtgagttgag
     4681 ccaagcatcc catatctagg aatggcagca aatgatttgg gtcttctcga aaatgcgcgt
     4741 tgcgttctgg atggacgatc actggtgtct ttcctagttg taacaactca aaaaagagcc
     4801 attctgcata taacggcact tccagtgtcg gaaattcgat tagaagatat gtatcatcta
     4861 aatctgtaaa taaaatttca tctcggtgga tgtcttcaat cagtgttccg gtaatacgta
     4921 cttcttgtcc ttccaataac gtcaaaggca attgtctttt ctctaattct gcttgtaacg
     4981 aagcgactag agaaatgact tgtgattttt cattgctgta ttttccatta ttgtgatggg
     5041 gtgtgcataa aatatgtgtg atcccttgtt gaatcgcttt ttcagccatg gcgatactgg
     5101 catctatgtt ttctgctccg tcatcgattc ctggcaggat atggcaatgt agatcgatca
     5161 tttctcttgt ctcctctctt tttatttctt aattcccgta gtaatagcca gcatctctgc
     5221 tgtgttctgc tccattgtat acaacaccaa ggactcgcgc ttgtaccata tctaacagtt
     5281 cttttgcttt agtcaatgat tcttttcgtg acgtattttc ccgtacgacc aataaggtgc
     5341 catccgtttt tgaagacatg atctgtgcat ctgttacggc aacgactggt ggcatgtcga
     5401 aaatcactac atcaaatagt tgtcttgctt ctgctaaaat ctgatccatt cttggcgaac
     5461 ttagtagttc tgatggattt ggtggtttag gtccacttgg cagaatcgac aaattgtcga
     5521 ctggtgttcg ttggatctca tctgctacac ttcctgatga actaagaaca gtacttaatc
     5581 cgcttgcgtt gttcaacttg aacgttttat agatgacggg cttacgtaag tctgcatcga
     5641 ctagcagtac ccgttgacca gatttggcaa aaacaacggc aatgtttgct gcagtggtgg
     5701 attttccttc tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg
     5761 cggaggcaaa ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg
     5821 ggtttgttaa tgtgatcaaa ctgactgcat gagtttgagt tgctttttgt ttctgtgttc
     5881 gtgccatttt tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc
     5941 gtttgtagct tttggtcgtg caggagctga tcctttttta cggataccag cgttcaattc
     6001 ttttgctgac atttctggaa ctgttcctaa gattgggaat cctaatgtat ccgtcacata
     6061 tttttcatct ttcactgtcc gatcaagtaa ttcgaataag aatgccagac ctacgcctac
     6121 catcattcct aacactaagc caatcactag attcaattta ttatttggcg aaacaggtgt
     6181 cgtattggca actgcacctg aaataatcga gattttatcg acattcatca cgtctttggc
     6241 gttttcttgg aaaacttgtg aagttgtatt agcgatctgt tgagcagtca ctgcattcgt
     6301 gcttattgcc tggatcgaga acatttgtga attttgtgac tgattgacgg agatcgcatc
     6361 tttgatttgt ccagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc
     6421 atttagtact aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt
     6481 attgacatca ttgacatttg ctgtgtctga ttgtggcagt gtcacgatca attgtgcttg
     6541 tgaactgtat tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc
     6601 aaacattgag atgatgattg ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa
     6661 gctgattgtc tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc
     6721 ttttaattct tttttcacac gatcaagttc ttgatcgctg actttttgat aggagatatt
     6781 atcttgcatg aagccttctc cttgcagctg atcttgcttg atcgtaccaa atgcctcccg
     6841 ataatctaaa gcgatttttt tcatttcatc aaagctcatg tctgttttca tattcgactc
     6901 tagagcattc aaaacggatt gatagttgct gacaccgttt aaactcaata ctttttttgc
     6961 tacaccttct acgatctttc tttgacgctc ttgtctacca tagtcaccat ttggatcttc
     7021 ataacgcata cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg
     7081 aaagtcatag ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc
     7141 atcaacgagt tcctttagcc ctttcatatt gatagagaca tagtgatcga ttgggatgtc
     7201 tacatatttt tgtagggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt
     7261 gatcttgtct tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac
     7321 gatcgttgtc tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc
     7381 ttgatcgacc cgacctaaat ctccagtatc aatgcccatc aatagaacag agaaagattc
     7441 ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta
     7501 agttttctca atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc
     7561 taatactaat acgataagaa ctgctaaaat aatcagaaat atttttttcc ctcttgattt
     7621 tcttcttcgt cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa
     7681 ctgtcaataa cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc
     7741 gtatgctgcg cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata
     7801 ttgatcgtaa gtagcggtgg cagtcacttg accatccttc aacatcgtat cgaataactg
     7861 atctagttgc gtgacttgcg cgatctgagt atcagcgaat cctagatatt gcataacaac
     7921 cgttttccaa ggactatctt taaaaaagct catattttca cgtacacgag cgacatctgc
     7981 acttgtcacc ttttctttga tgacgacatc gtctacagct tgctgccagt ttgatacatt
     8041 tttagtaaat aaatcgttga cagctgtctg gatcttcagt tttgattctg cactatctaa
     8101 ctgtgtcacg attgctttct tctcatcttg gattgcaaca gcttctttag gcaattctga
     8161 ttctttgatg tcgtaatcat ctgctgaaag tttgacagag gatagcttat ttgacaaact
     8221 tgtgattgtt tcttctgtta tttcagggtt tagaaaattt ttttcttttg tactatacag
     8281 cttgtctact tcttttgata aagaagtcaa ggtgtctttt tgattggtga tggcagattc
     8341 tgcataattg atccgttgct gatgttgccg ctgattattg atatggtaac caaacaaaca
     8401 gatcgcaata gcacctgcac ctaccgccac agcagtcaat gacttgacga atggcttttc
     8461 catagattca ctcctaagta ttgatgatag ataaattaag atataattca catacataaa
     8521 aatgacagct ttttatttta aaaaacataa ttaaattaat atattcacag aaatatttgt
     8581 aaacaagttt tccctatata atcataccac aaattgtttt cttgactagg gcaatttacg
     8641 atttaaaagt aaataaaaca ttaataagat aacaattaat cgatttcatt tttatttcct
     8701 taattttcat
//
LOCUS       DAYYNQ010000109         8284 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000109 DAYYNQ010000000
VERSION     DAYYNQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.110"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACHBJ1_002510"
     CDS             <1..231
                     /locus_tag="ACHBJ1_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFM9431417.1"
                     /translation="LIILTDLIGGTPNNVSIKQLNNNRIQIIAGINLPLLLELLMAQS
                     AGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            281..745
                     /locus_tag="ACHBJ1_002511"
     CDS             281..745
                     /locus_tag="ACHBJ1_002511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM9431418.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            759..1508
                     /locus_tag="ACHBJ1_002512"
     CDS             759..1508
                     /locus_tag="ACHBJ1_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM9431419.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1508..2338
                     /locus_tag="ACHBJ1_002513"
     CDS             1508..2338
                     /locus_tag="ACHBJ1_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM9431420.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2338..2985
                     /locus_tag="ACHBJ1_002514"
     CDS             2338..2985
                     /locus_tag="ACHBJ1_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM9431421.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(3083..3859)
                     /locus_tag="ACHBJ1_002515"
     CDS             complement(3083..3859)
                     /locus_tag="ACHBJ1_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9431422.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            4003..4962
                     /locus_tag="ACHBJ1_002516"
     CDS             4003..4962
                     /locus_tag="ACHBJ1_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM9431423.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            5079..5387
                     /locus_tag="ACHBJ1_002517"
     CDS             5079..5387
                     /locus_tag="ACHBJ1_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM9431424.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            5414..5713
                     /locus_tag="ACHBJ1_002518"
     CDS             5414..5713
                     /locus_tag="ACHBJ1_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM9431425.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            5887..6438
                     /locus_tag="ACHBJ1_002519"
     CDS             5887..6438
                     /locus_tag="ACHBJ1_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM9431426.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            6584..6877
                     /locus_tag="ACHBJ1_002520"
     CDS             6584..6877
                     /locus_tag="ACHBJ1_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM9431427.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            6870..7166
                     /locus_tag="ACHBJ1_002521"
     CDS             6870..7166
                     /locus_tag="ACHBJ1_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431428.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            7237..8241
                     /locus_tag="ACHBJ1_002522"
     CDS             7237..8241
                     /locus_tag="ACHBJ1_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM9431429.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         2929 a         1203 c         1547 g         2605 t
ORIGIN      
        1 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
       61 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
      121 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
      181 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
      241 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
      301 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
      361 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
      421 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
      481 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
      541 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
      601 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
      661 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
      721 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
      781 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
      841 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
      901 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
      961 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     1021 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     1081 gtattatcat gcgcatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     1141 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     1201 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     1261 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     1321 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     1381 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     1441 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     1501 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttaataa
     1561 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     1621 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     1681 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgacttc
     1741 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     1801 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     1861 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     1921 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     1981 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     2041 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     2101 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     2161 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     2221 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     2281 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     2341 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     2401 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     2461 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     2521 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     2581 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     2641 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     2701 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     2761 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     2821 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     2881 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     2941 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     3001 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     3061 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     3121 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     3181 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     3241 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     3301 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     3361 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     3421 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     3481 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     3541 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     3601 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     3661 cccttaatcg ttcaactgtt ccactttctg ctaaaagttt aaaagctttt cgaatggtaa
     3721 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     3781 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaaa tataatggtc
     3841 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     3901 tattaaaacg tcgattgtaa aattaagcta gacaactaaa aagatcattt gtgctacact
     3961 caaaatagtt ccgaccaaag aattaataag gagtgagtac aaatgactta cacccatctt
     4021 acaacagacg aactagtaat catagagtct tatttcaata tcgagaaacc tgtcgctctt
     4081 gtcgcacaaa gtttaaatcg ctctagacaa accatttata atgtttattc gtttctcaaa
     4141 caaggaaaaa aagtgctaga ttattaccgt caatacaaac aaaataaatc tcgttgtgga
     4201 cgacgttcaa ccgttttatc tgatgaacaa tcaacatatg ttcaagaaaa ggtagctcaa
     4261 ggatggtcac ccgatgtgat tatcggccgt ggcgagtttc caattgattg ttctgttcgt
     4321 acactctatc ggatgtttaa aaaaggcctt tttgacacac agtcgttacc tatgaagggg
     4381 aaacgtaaac cgaataatca tcaagaaaag cgtggaaaac aggcctttcg cagaacgatt
     4441 catgaacgga acagtctcta tccacacctg actaaagaat ttggtcatct cgaagtcgac
     4501 accattgtgg gagttaaaca caagagcgct gttattacat tggttgagtg tttatccaag
     4561 gttattatta ctttgaaacc gacaggcaga cgagccgaag atatcgaaaa caggttaaat
     4621 gattggttca aatccatgcc taaacacttg tttaaatcca tgacatttga ttgtggaaaa
     4681 gaattttcca actggcgatc tataagtaac tcgaacgata ttgccatctt tttcgcagat
     4741 ccaggaacac cttcgcaaag agggctaaat gagcactcaa acggcttgct tagacgagat
     4801 ggcttaccta aaacaatgga ctttagggac acggatgaaa cctttattca atccgttgcc
     4861 tcgaaaagaa accatattcc aagaaaatca ttaaattatc gaacaccatt ggaagtattt
     4921 ttgagctgca tagataaaga catattgtct agcttaattt gacaaatcag atagtataaa
     4981 atgaatcaaa ttgaatatca aatatatatt atatccatga agcccaatct tctttaatag
     5041 aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat aaaggacaac
     5101 gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct ttatgttgga
     5161 atctaatcga tcaaaacgtt caaaagcaat ttcatttaga ctatcttcaa atctttgaat
     5221 tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaacca gaagcaagaa
     5281 aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa atatgggtca
     5341 ttgattcggg tgagtatcaa acgatgttat taccagaaga atactgatat ctaaaagaaa
     5401 ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg cacagctagt
     5461 cactactcct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg aaattgaaat
     5521 gggtatgctt cctgaagatg ttactctacc acgtttagtg atgacacatc tttatattta
     5581 ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata ttaccagcca
     5641 gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc agattggagg
     5701 aacgaatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa gatatcctta
     5761 cagcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt ttcaactagt
     5821 attgacactt ataacggcaa tggtgattga attgcttgaa caaaaattag cataggagaa
     5881 aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg agatcctcat
     5941 catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga agcggaagat
     6001 aaattagcgg tagtcattac tattcgtcca acagatacag aaccatttac gaatgatttg
     6061 agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt tattgctgtg
     6121 aatctttttt cgtatacaaa agcgaaaagt cctcgggtat ttcctcgtgg aaatgatgaa
     6181 caatctttgg aaatattaac aactgttttc acagaaaaga aaatcaacac cattattttt
     6241 gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcgaa gaccatttac
     6301 gatcaattga gtgcgaaaca aaaaaagatg accaaagtat tgataaatgc agaaggtaag
     6361 ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga tagtcaatta
     6421 ttatttcaag aaaaataaca ttttattttc ttatgttact tataagtagt tttacatgct
     6481 ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat gtacccagta
     6541 atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga ttgatgaaac
     6601 ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg aactggattc
     6661 agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt ttccaatgaa
     6721 aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac aggaaaaaac
     6781 tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga ttcaaaaagt
     6841 aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta ggtggtttaa
     6901 tttacggtgg atattggtta tataaatata tgcttgcgca tccgaatata gataaaggaa
     6961 aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact aagaaaaata
     7021 atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga agggaaattt
     7081 ctactagttt taatccatct gaaagccctc aaggaagaga tcgttgtcat gctggctatc
     7141 gacattcttg ggaaaggatt aactaaatga acaccattga gattaattga ctgataaaat
     7201 aagtacgcga gggagaaatt gaaaggggga aataaaatga aaaaatggtt gttattacta
     7261 attggaatgg ggattttatt cactggttgt acatctgaga atacgaggac taaagagact
     7321 gtagaaactg atacaacaat tgaggctaaa aaaagtaaaa cgcaatcaac tgaacaaact
     7381 acaacagaaa tgaaacaatc tgtggaagca agtacaagtg aaagttcaat cattgaatct
     7441 tcttctgaag aggttaaaaa tactttgtgg gatagtgaga aatctactca actagcaatt
     7501 tttgtcccac aatggggaaa gacattaggt caagaatata aaagctacaa tcctcagaac
     7561 aatgtaagtc tgtatggtac gcctttacca agtgcagtta ttgatggaaa ttggaaaatg
     7621 gcaatcaatg aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat
     7681 ttcaatttag tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgta
     7741 tatttctttg gctttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggc
     7801 aatgataaaa actacatgta cttcaatgaa actcagaacc aacagttaaa acaaggtttc
     7861 attgatattg tgaatggaca aactcctcaa actccagtag tagaagaatc tactcaacaa
     7921 gcaacaacta caacgacaca atctacaagt agctcagcaa tttcaagttg ggaagctgct
     7981 aaagaaactg tactaaataa taaagcttta tggttgtatg attctcctga taaaaatgca
     8041 tcaaatacta atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat
     8101 ggaagctact attcaattaa tattgtaacg gatgataatg gaaatacaac aatggctggt
     8161 ggaaaacaat tccgtgttta tacagatgga agaatttctc aacgtattgg aatgactaac
     8221 aattttcaaa tgattaaata attgaaaata ttatttatca aagaaaaact gattttgaga
     8281 aaaa
//
LOCUS       DAYYNQ010000110         8081 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000110 DAYYNQ010000000
VERSION     DAYYNQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.111"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..296)
                     /locus_tag="ACHBJ1_002523"
                     /pseudo
     CDS             complement(93..296)
                     /locus_tag="ACHBJ1_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            292..459
                     /locus_tag="ACHBJ1_002524"
     CDS             292..459
                     /locus_tag="ACHBJ1_002524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431430.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(502..1077)
                     /locus_tag="ACHBJ1_002525"
     CDS             complement(502..1077)
                     /locus_tag="ACHBJ1_002525"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM9431431.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1232..1758)
                     /locus_tag="ACHBJ1_002526"
                     /pseudo
     CDS             complement(1232..1758)
                     /locus_tag="ACHBJ1_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2215..3726
                     /gene="gadC"
                     /locus_tag="ACHBJ1_002527"
     CDS             2215..3726
                     /gene="gadC"
                     /locus_tag="ACHBJ1_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM9431432.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3739..5139
                     /locus_tag="ACHBJ1_002528"
     CDS             3739..5139
                     /locus_tag="ACHBJ1_002528"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM9431433.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5304..6739)
                     /locus_tag="ACHBJ1_002529"
                     /pseudo
     CDS             complement(5304..6739)
                     /locus_tag="ACHBJ1_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7132..7725
                     /locus_tag="ACHBJ1_002530"
     CDS             7132..7725
                     /locus_tag="ACHBJ1_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM9431434.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
     gene            complement(7709..>8081)
                     /locus_tag="ACHBJ1_002531"
     CDS             complement(7709..>8081)
                     /locus_tag="ACHBJ1_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9431435.1"
                     /translation="IKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPF
                     DNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRN
                     QRLAQRILDNELGCANASEAV"
BASE COUNT         2519 a         1389 c         1475 g         2698 t
ORIGIN      
        1 ctaatgaaac aatttctcaa tctttttacc actccaaaca gggagcgtca ataattttgt
       61 gtaaataact cgtccttctg caaaataatg ggttactcag taaacattga agctaatgta
      121 tccgtcactt gttggaagcc tttatggctt ctatttaaga atttttgatt gtatgtatca
      181 aagatggata ctaggaaacg ctctagtgat tcttcatttt gaaactgctc ttttctgcgg
      241 ctgtatttct tgatttgttt attgaaagat tcgattgtag aaccagaagg aatggagaac
      301 acttttgaac tttacaacaa aactactgtt gataaattaa ttccaatttt agttccaact
      361 gatttaaatg gtcatgatgt tttacttcgt tttaacggat ttttaggtga ggattataga
      421 tacctaaaaa tttctgatcc tcaaccttta acagaataat aaaaaacgtg taatcaatta
      481 tttgattaca cgttttttat ttcaatcttc gttgtagtag tggttgcgac ataccgaaag
      541 gtaatcttca ttacctccta ctacaatctg ttcacctgaa taaactggaa taccatcaac
      601 aatacgagca ttcattacag cctttttatt gcaaaaatgg caaatagttt taatttcctc
      661 aattttatca gcaaatgtca ataatgcttc ggatcccttg aagagattat tttgaaaatc
      721 attcttcaat ccatagcaaa ttactggtac ttcctcattc attacgataa gtgatagatc
      781 aataatattc tctctactta aaaattgaga ctcatcgacc aaaatacaat caacttttcc
      841 gtactgatca ataatctcca ctaattctcc gtacacatcc atttcagaag taattgcaat
      901 agctggtttc gaaacaccta tacgactctc aacttttcca aatcctgatc gagtatctat
      961 tgcgcttgtc attatgataa ctctcttccc ttgttcttca taattatgag ccactttcag
     1021 tagctcaata ctctttccac tattcattgt tccatatctg taaaataatt gtgccattaa
     1081 atttattccc ttcttttaca attcaattat tccttaattc catctgcttt tatgcagctg
     1141 ctcgtttatt ccttgatatg tctataacat ctactatccc taaaaccaat ttataagcca
     1201 ttagagatgg tattcctgaa catctctcat attgtttttt gagtggtata tcatctgaaa
     1261 aactgaagga atcatgaaaa tagcgacata cttatcattt agagttctaa ctttaaatgc
     1321 atttacttct tcagtcagca ctttagtcat gttggacatg gtttggggcg tgtagtgatg
     1381 cccatacatt ttctcaatca agtctgcgat ttccgacatg gtaatacctt ttcggaagag
     1441 gtgaatgacg gtctcctcta atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg
     1501 cttgaactcg ccattgcggt cgcgcggaat ctggagatga agttccccat actcggtctt
     1561 gaccgtacgg tcataggagc cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta
     1621 cttttcgtaa tcgaggaaag ccgttaactc cgttttgaga agcgtattga cagctttttc
     1681 caaatggaca cggaataatc cattcaaatc gcctttgttc gctagagtct ttaggatttc
     1741 tgtagtaaaa tcgttcatat ggaagtcctc ttttctgtga atgtgttgtg gtaactctat
     1801 tctacagaag ggacttcctt ttttctattt acacaaaata ttttacactc tcaacaaaag
     1861 tatcttcgat taatacttgc tcaagttatg tgcagctacg aaaaagtagg taccgaggta
     1921 ctttcttttt cgcagctttt tttggactta tgagaagtac attcgctgga agaatacgat
     1981 ataaaacgag ctttttcact aaagatatta atagagtgaa agaaacaagc ttttgttgtc
     2041 agttttttca cttttctaaa aatgaaaata aaaaattctg gaagtggaga agaattttgc
     2101 ttgctatact ttgtcttgta gaaaagaggt agacaaaata atgcaagctc aaataagcga
     2161 ttttccataa ccttggtagg aatgacaaat taaataggaa agggaagtaa taatatgaat
     2221 aaaaaaaagt tatctctatt cggttttttt gccctgacag cttccatggt tttgactgtt
     2281 tatgagtatc cgacctttgc aacatcacaa ttacatttgg ttttctttct tcttttggga
     2341 ggtattttat ggtttctacc agttgcttta tgtgctgcag aaatggcaac agttgaagga
     2401 tggaaagatg gtgggatttt cagttgggtt agtcaaactt taggagaacg ttttggtttt
     2461 gcagctattt ttttccaatg gtttcaaatt acagtaggtt ttattacaat gatctacttc
     2521 atactaggag cgctgtcgta tgtaattaat tttccagagc ttaatacaga tccatttatc
     2581 aagtttattg gtgtattagt cattttctgg gtactgactt tttcacagtt gggcgggacc
     2641 aatcgtaccg ctaaaattgc taaagcagga tttgtcattg gaattgttat tccttcgatc
     2701 attctctttg gtttagcagc agcctacttt atcggcggta atccgattca aatggctatt
     2761 aatgcgaaag cttttattcc tgacttttct cagctttcaa ctttagtagt atttgtatct
     2821 tttatattgg cttatatggg cgtagaagct tcagcatcac atattaatga gctggaaaat
     2881 ccccaaagaa actacccatt ggcaatgttt ttactagtga ttttggccat cgccttagat
     2941 gcaatcggcg gtttctcagt agcagcggtt attccacaac atgacttgtc tctcagtgct
     3001 ggtgtaatcc aaacatttga aacgcttatt ttacatttta acagtcattt acattggttg
     3061 gtaaaagtga ttgctctcat gattgcctgt ggcgttatgg gtgaagttag ttcttgggta
     3121 gttggtccat cacgagggat gtttgcagct gctcagcgag gtctgttacc agttttcttc
     3181 cggaaaacta ataaacaaga cgttccagtt ccgttgatta tgatacaagg aattatcgtt
     3241 actatttggg cagcagtctt aacttttggt ggaggaggca acaatttatc attcttagta
     3301 gcaatctcct taactgttgt catttatctc gtaggatatt tactattttt cctaggttac
     3361 tttgtattag tttttaagaa aaaagactta aaacgaactt acaatgtacc aggaaaaact
     3421 gttggcaaaa cgattatcgg tggaattggt tttattttat cagtgttcgc attgcttatc
     3481 tcttttgtac caccagcttc aattgcagct agtgagcacc gaacatacca aatcatacta
     3541 cttattagct ttgtagtgac tgttctttta ccttttatca tttatgaagt acatgataaa
     3601 agtgctcaca caactattga ggaacctact cacgtgaaag ctgaagacgt taatcccgct
     3661 gtttatccag ctgctagagg agagcaccac gttacaaaaa atgatgaaca tattttaaaa
     3721 cattaggagg aattcattat gttatacgga aaagataatc aagaagaaaa aaactatttg
     3781 gaaccaattt ttggctctgc aagtgaggat gttgacttgc ctaaatataa gttaaacaaa
     3841 gaatccattg aaccacgaat tgcttatcaa ttagtacaag acgagatgtt ggatgaagga
     3901 aatgcgcgat taaacttagc tactttttgt caaacgtata tggaacctga agcagtgaaa
     3961 ttgatgaccc aaacgttaga aaaaaatgca attgataaat cagaataccc acgaacaacg
     4021 gaaattgaaa accgctgtgt aaatatgatt gctgatttat ggcatgctcc aaataatgaa
     4081 aaattcatgg gaacttcaac gatcggctct tcagaagcct gcatgctggg tggtatggcc
     4141 atgaaatttg cttggcgtaa acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa
     4201 cctaacctgg tgatctcttc tggttaccaa gtttgttggg aaaaattctg tgtatattgg
     4261 gatgtggaac tgagagaagt cccaatggat gaaaaacata tgtcaattaa tctagatact
     4321 gtcatggatt atgtggatga gtacacaatt ggtattgtag gtattatggg tattacttac
     4381 actggtcgtt atgatgatat caagggtctg aatgatttag ttgaagctca caataaacaa
     4441 actgactata aagtatacat tcatgttgac gctgcatcgg gtggctttta tgcaccattt
     4501 actgaacctg atctagtttg ggattttcaa ttgaaaaatg ttatctcaat taattcttca
     4561 ggtcacaaat atggtttggt atatccaggt gtgggttggg tcttatggcg tgaccaacaa
     4621 tacttaccag aagaattagt atttaaagtt agttacttag gtggagaaat gccaactatg
     4681 gctatcaact tctctcatag tgcagcacaa ctaattgggc aatactacaa ctttgtacgc
     4741 tatggctttg atggttatcg tgatattcac caaagaactc atgatgttgc tgtttattta
     4801 gccaaagaga tcgaaaaaac tggtattttt gaaatcatta atgatggatc agaattgcca
     4861 gttgtgtgct ataagctgaa agaagatccc aatcgcgaat ggacactata tgatttatct
     4921 gatcgtctgt taatgaaggg atggcaagtc ccagcctacc cattacctaa agacttggat
     4981 caattaatta ttcaacgctt agttgttcga gcagactttg gaatgaacat ggctggtgat
     5041 tatgtacaag atatgaacca agcaattgaa gagttgaata aagctcatat tgtttatcat
     5101 aaaaaacagg atgtgaaaac gtacggcttt actcactaaa attcttcaga gaaatataaa
     5161 agaagttaat taactaataa aaggagttgt gattaccaca cagctcctta aatctatttg
     5221 aataaatata tgatattcat ttgttacact ctcaatctta gctgttgttc tattcatcat
     5281 tactcttatt gtaagtagtt cagtcaaatt tgcttgttca attgatcgat aacagtatga
     5341 attgtttgaa aagtaatcgg atatataatt gttttctcac tacctaagcc ttcttggtat
     5401 aaagaactat cgcaaagtat aatccgctga gtacaaaaga aagtgtcgtc tactatttct
     5461 ctaagttgtt tacaaatgat attactaata gaaaagtgtt ctcccaaaat gtcttctaac
     5521 tgttgtcgat agtataaata cctaaagtct gacggagcta ctatgaacac ttcaattgga
     5581 gatttccgtt tatgctcatt taaaagactt aaactaatgg ttagtttacg aatagaattc
     5641 tcattccaac gcagaaataa attgttttgt tttcgccatt gttcaagaat ttcatgaact
     5701 tctaagtaca aagcgcgttg ctcttcattt aacaagtaag tcttatcagg ttgaaatatc
     5761 tgaatatcag cccaaagtga gcggataaac gggagaagtg ctttatcaaa aagtatctga
     5821 ttttcttcag atatattcag agataagata agtttttgac tgagctcaat caccaaaggg
     5881 ttatctgcta catggttttg ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta
     5941 tcgcatagat aatttctaga gttgaataga aagtagatat aaaggcattc atcttcgtca
     6001 tgacataaag agaaagaaag ttgctctaat cctttctgta ttagctgaaa caagggagta
     6061 tgtcgggcaa ctcttttttc ttcctgacta aaatatattg ggttagtctt agatctctgc
     6121 aaaccgatag cgatacctaa taaaataaat tgtttttcaa ctgtagggta acttcttgaa
     6181 aaaaatgttt ttctacataa tcaatcaatt gttgaccgct ttgaataatt gatttattta
     6241 gctggatcaa ctctgtccga tttgtctcat agattatcgt tagtaacaaa cttcgataat
     6301 ccttttcagg aatttcaaaa tggccatcag catttaagta ctgcatttgt ttaaaaaaat
     6361 taatcaactc atttcgtaag ttgtaaactt tgcttaacga aatgaaggta gtatcagcta
     6421 tattactcca attgtgttcc cctgataaat aatgtcctag tacattgaaa aagtcagagc
     6481 gctgatagat gagttgagtg agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa
     6541 agctacttct agatttctcc actgaaataa tttgctgatg gagtatgtcg gtaatgcatt
     6601 ccaagtcgtt agtgacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     6661 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     6721 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataactc
     6781 taccaatcgg aatagtcctg taatatattg aatcaaaaat ataaattata aaataattag
     6841 atggggtatt tggtttaagt atcccttata tagtaataaa tttataggtg tgattctcga
     6901 attaataccc gaaaatgtga gagaattttt atggattttt taggatacaa attaattatt
     6961 tttgctttta tcctgattac cagtccagta ctgtcggtat cccattttgc agcaaaatca
     7021 tgttgataaa gtgacaagaa gactgatgag atatattatc caaattgaga agataattaa
     7081 tcggattctt gagtataccc tactttttaa gaaagtaggg tatacaagcg tatgaataaa
     7141 gacataactt tccctgtgat ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac
     7201 gattataatc ccaatcgagt agcgtctccg gaaatgaact tattggagct ttctatcatt
     7261 gaagatggct ttactcaacc attagtttgt tattacaagg aaaaagaaga gaagtatatc
     7321 ttggttgatg tgcttcatag gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta
     7381 ccggtaacag ttattgataa acccatagaa gaacgtatgg cttcaacaat tcgacacaat
     7441 cgtgcaagag ggacccatga aataagagga atggaaattg tcatccaaag attagtaaaa
     7501 aatggatggt cagacaagag aatatccaaa gaattaggca tggacattga agaaattttt
     7561 cgctttaagc aagtgtctgg tggaatggca acagtttttt tgacaaattt tataaggtgc
     7621 agaacttctt tccgtatgct attccgattg tccttgacaa tgagcctcct cggatgtgac
     7681 gatcttcggc aggagctaac agttaaagtt agactgcctc gctagcgtta gcacatccga
     7741 gctcattatc aaggattcgc tgcgccaatc tctggttacg gtaaccaacc ggtgtctcgt
     7801 agccaagtgt accgtgcaac cgaaggtggt tccaccaatt tacatagtca aataactcca
     7861 aatccaattg ttgtaaggtt tcaaatgtgt attgatagac aaattctact ttcaacgact
     7921 tataagttga ttcagctacg gcattatcaa aaggacagcc tttatgactc aatgatcgat
     7981 tgatgtcaaa agttgttaat aattcatcaa tagcttggtt atcaaactct tttccacgat
     8041 cagtatgaaa aatctcaacc tctgtcagag gttgtttgat a
//
LOCUS       DAYYNQ010000111         8022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000111 DAYYNQ010000000
VERSION     DAYYNQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.112"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..199)
                     /locus_tag="ACHBJ1_002532"
     CDS             complement(<1..199)
                     /locus_tag="ACHBJ1_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431436.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="ACHBJ1_002533"
     CDS             complement(362..1996)
                     /locus_tag="ACHBJ1_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431437.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="ACHBJ1_002534"
     CDS             complement(2000..3373)
                     /locus_tag="ACHBJ1_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431438.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="ACHBJ1_002535"
     CDS             complement(3389..3628)
                     /locus_tag="ACHBJ1_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431439.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="ACHBJ1_002536"
     CDS             complement(3810..6485)
                     /locus_tag="ACHBJ1_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM9431440.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="ACHBJ1_002537"
     CDS             complement(6668..7591)
                     /locus_tag="ACHBJ1_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431441.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2359 a         1578 c         1206 g         2879 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaac tttaaatacc tttgataaaa
     7921 aatattgatg ttttttctct cattgtttac aaactagata gcttatgtat tcttattaaa
     7981 aataatagat atttcgaaca tcatcactct catctactta at
//
LOCUS       DAYYNQ010000112         7806 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000112 DAYYNQ010000000
VERSION     DAYYNQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.113"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHBJ1_002538"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="ACHBJ1_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM9431442.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="ACHBJ1_002539"
     CDS             complement(1378..1896)
                     /locus_tag="ACHBJ1_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431443.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="ACHBJ1_002540"
     CDS             complement(1912..2238)
                     /locus_tag="ACHBJ1_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM9431444.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="ACHBJ1_002541"
     CDS             complement(2251..4230)
                     /locus_tag="ACHBJ1_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9431445.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="ACHBJ1_002542"
     CDS             complement(4251..4565)
                     /locus_tag="ACHBJ1_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM9431446.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="ACHBJ1_002543"
     CDS             4733..5026
                     /locus_tag="ACHBJ1_002543"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM9431447.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="ACHBJ1_002544"
     CDS             complement(5104..6216)
                     /locus_tag="ACHBJ1_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM9431448.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..7217
                     /locus_tag="ACHBJ1_002545"
                     /pseudo
     CDS             6369..7217
                     /locus_tag="ACHBJ1_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7570..>7806)
                     /locus_tag="ACHBJ1_002546"
     CDS             complement(7570..>7806)
                     /locus_tag="ACHBJ1_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM9431449.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2495 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatc
//
LOCUS       DAYYNQ010000113         7498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000113 DAYYNQ010000000
VERSION     DAYYNQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.115"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBJ1_002547"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="ACHBJ1_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM9431450.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBJ1_002548"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="ACHBJ1_002548"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM9431451.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="ACHBJ1_002549"
     CDS             complement(2298..2960)
                     /locus_tag="ACHBJ1_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM9431452.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="ACHBJ1_002550"
     CDS             3144..4790
                     /locus_tag="ACHBJ1_002550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM9431453.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_002551"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="ACHBJ1_002551"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM9431454.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7498)
                     /locus_tag="ACHBJ1_002552"
                     /pseudo
     CDS             complement(5971..7498)
                     /locus_tag="ACHBJ1_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2353 a         1370 c         1385 g         2390 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg
     7441 ttcagatagg aaaagaaaaa tatctagttt tgtattgctt tttttcttca agataaac
//
LOCUS       DAYYNQ010000114         7439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000114 DAYYNQ010000000
VERSION     DAYYNQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.116"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..266)
                     /locus_tag="ACHBJ1_002553"
     CDS             complement(<1..266)
                     /locus_tag="ACHBJ1_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431455.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIV"
     gene            complement(384..644)
                     /locus_tag="ACHBJ1_002554"
     CDS             complement(384..644)
                     /locus_tag="ACHBJ1_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431456.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(781..1194)
                     /locus_tag="ACHBJ1_002555"
     CDS             complement(781..1194)
                     /locus_tag="ACHBJ1_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM9431457.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1650..2135)
                     /locus_tag="ACHBJ1_002556"
     CDS             complement(1650..2135)
                     /locus_tag="ACHBJ1_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431458.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2274..2489)
                     /locus_tag="ACHBJ1_002557"
     CDS             complement(2274..2489)
                     /locus_tag="ACHBJ1_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM9431459.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2713..3513
                     /locus_tag="ACHBJ1_002558"
     CDS             2713..3513
                     /locus_tag="ACHBJ1_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9431460.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3532..5400
                     /locus_tag="ACHBJ1_002559"
     CDS             3532..5400
                     /locus_tag="ACHBJ1_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9431461.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5393..5884
                     /locus_tag="ACHBJ1_002560"
     CDS             5393..5884
                     /locus_tag="ACHBJ1_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM9431462.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5872..6426
                     /locus_tag="ACHBJ1_002561"
     CDS             5872..6426
                     /locus_tag="ACHBJ1_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM9431463.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6444..7439
                     /locus_tag="ACHBJ1_002562"
     CDS             6444..7439
                     /locus_tag="ACHBJ1_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM9431464.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2641 a         1196 c         1422 g         2180 t
ORIGIN      
        1 acaatggcaa agatcgtaag agagatcata agccccaatg aatgactaaa aagaaaagca
       61 gaatcacatg ccatatcatc ccatatttct tcaagaaaat aaataagcgg gaaaaaggga
      121 aggaatagta gaacatcgat ccagcaccat ttttttcttc ttttgaaata acataaaaca
      181 ataaacatga acgtgcaggg aagtaaaaac aaagctttac tcataactaa caaaatacca
      241 gctcctttta tttttttctc ccccacattc aaagagtaac aatggtatgt acttaataat
      301 agaaaaatgg atcaacttgt atgaataaca gctgaaaaga tctgcaccaa ataaaaaact
      361 tccagccaaa actgactgga agtttatttt tttctgaaat attctaacac aaatatgaaa
      421 aagatggccg aagctaataa gccgatttcg tagcaaaaca atgagatacc agagtcgtaa
      481 taataaaatc ctctaatctt atggataaaa tagatcatcg gacaagcaga taagagtaag
      541 tatttgttag gccttttgaa taagtaaaag aaagcactgg tcattatgat aaatggtatc
      601 caaaacaaaa gtttacttaa gacaatcaaa tgatcaactc ccatattttt taccaagaga
      661 gatcataaca tctttatcgt aaaaacaaaa tatttttttg ctcattttga aaatttaaac
      721 atactcgttc ttatccaaat atataaaaat cgcccctcat tcatcgagag gcgataagtt
      781 ctaatttaac tcaatttttt ttgtttcttt tggctgataa gctgttttag gtaataccaa
      841 tttcagcaca ccgtttttaa aggttccagt gattttttct tcatcaacat taggaagata
      901 gaatgaacgt ttgtaagaga ccgaatgtct ttctttccgt acatagttgc catcttcttt
      961 atcttctgta tgggactcat gattggcgga gatcgtcaaa gtattatctg aatattcaac
     1021 tgtcgtgtct tctttgtcac agccaggcat atcagcagtt agtttgtatt ctttttcatt
     1081 ttcgactaaa tctacttttg gataacttga aacacctaaa aaatcattga atgctggact
     1141 aaacacgtca ttgaaatcag ggaacatgtc gcgaatgcta ggtacatttg ccatttttga
     1201 tcatcctttc aataagtatt ttttatttat ccctatttac gtaataactc tattttgaaa
     1261 aaaatgcaag tataatggtt agctttgtga atatttcatc taatacgata caaaggctat
     1321 ctttctaatt tgaaaaaggg atcattaaaa taataagaag aaaatatgtc aaaaggtaaa
     1381 aaagtaaatt cttctatcca aatatagaaa aattgaatct agaaaaccgc ccgacaataa
     1441 atttcattaa ctcagttgac cgaaaatgaa gtagatacca aaaatttatc aagagagcaa
     1501 gtagagaagt gagtatattc ataagagaaa tagctgtaac gaattattag gttaatgcaa
     1561 atgcttattt atactccgta taactttctc agaaaaaatg ataccaataa tgacttgcta
     1621 gtactgtaaa tccgtataat atcatctaat caaaatggtg ttccatttgc atctgtcatc
     1681 gtaatcgatc cttcgggtga cactcgataa aaaccaacgc ttccagatcc tccttgggaa
     1741 cggatttgtt tgctggaagc tgtgaacaaa taatctcgat caattttttg ataaaagcta
     1801 ataacaatat cgtcactttg taattcggaa cggtgggcaa taagaaatag aagtgctttg
     1861 tcagagtttt ctaatggaag agaaggtaca gtgctttctt gcatttcaga tgttgaagaa
     1921 atgctaaacg actgaatact tgattggcta tgttgcgaat ctacattacc agtagaagta
     1981 ttagtaatgg aagtagtaga agtactttta gttgaaccaa cctcagttgt tgaaatggct
     2041 gctgaagatt ctttaacaga attctttgtt ttatatccac aggcagaaaa aagtattaca
     2101 gttcccaaca agcctgcgta aatcccttta tgcatgatag ctcctcctaa aatagattat
     2161 ctttattcag tatatcactt aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc
     2221 ccaataaagg gaaggttcat gaagataata acttttttaa taattatagc tttctaatat
     2281 ctttcatcaa cttttctagt aatcattggg aaaaatttca agcagtagat cgcacaaata
     2341 cctacgacga tataaatgat cttcgcaaaa atagttgttg aaccaccagc aatcatagct
     2401 actaaatcaa gttcgaacaa gcctaccaac agccagttca accctcccac aatcaataaa
     2461 gttaatgcaa tagcatctaa agttttcatt agaattctcc tttcttaaat gataaaacaa
     2521 ttatatatca aaaagagaaa cattgcttat ataacgctca ggaattttag caatctatgc
     2581 aaaaagatga aaattttttt tgctcttcca ttagtacaca ttttttagct gaagctgtaa
     2641 atttcatgtc ttaatttttt ttagtgagct tttatactgg atttgtaaga aaaaagaagg
     2701 agaggatcac atatgtcgga ttggttggat attgcaggaa aagtagtaat tgtaactgga
     2761 ggttcctcag gaattggaag aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta
     2821 gcgaattttg atgtaactga atgccgtata cagcatgaaa acctactatc tttaaaagtc
     2881 gatgtgtctt ctaaaacgga catagaagaa gggatatata aagtaatgaa acatttcaag
     2941 acgatcgatg gtctagtcaa taatgcagga ataaatattc cttctcttct aatagataga
     3001 aatcatccaa aaagcaagta tgaattatca gaacaagttt ttgataagat gatcgctgtc
     3061 aatcaaaaaa gtgtatattt gatgagccaa gcagttggaa gaatcttggt gcaaaaggga
     3121 tcaggagtga tcgtgaatct ttcttctgaa tcaggattgg aaggatcaga aggacagagc
     3181 tgttatgctg ctacaaaagc agcgatgaac agttttactc gttcttgggc aaaagagtta
     3241 ggaaagagga atgttcgtgt ggtaggtgtt gcaccaggga tactggaaga aaccggtttg
     3301 agaacacaag aatatgaaga agctctttct tatacgagag gtataagcgt cgaacagctg
     3361 agaaacggtt acagtcgaac aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa
     3421 gtggcggatc tagtctgcta ttatctttca gaaagatcaa gttatatcac tggagtcacg
     3481 actaatattt caggaggtaa aacaagagga taaaggatag gggggacgca cgtgacgcta
     3541 gtaaacagat ggtaccaatt attgaaatta ctagtcactc acaagaaagt ttcaatccag
     3601 gaagtgcaaa aagaaatcaa aaccagtaat caaaccataa aaaaagatat tggggagttg
     3661 aatgaagaaa ttgccggtat tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa
     3721 atttatgatt ttgatttttt tgatgagatc atgcaaggaa aattgaaaac agccagtgat
     3781 ttcaattcgg catccaaaag aatcgcttat ttactaaata ggctgatcga aacagaggat
     3841 ttcttacgta tggatgattt gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag
     3901 gatctaaaga cattaaaaga gatgatcaat gattttgatg taaagattac aggcacacca
     3961 aataaaggac taagaataat aggagatgag ctagaattac gactactttt attttatttt
     4021 gtttctccct acttttcaga gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc
     4081 aaacattttc agaagaagac gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt
     4141 atcggtattt ctttgaatag aatctctagt aactatctgt tgaagaagcc catcaatcgt
     4201 tacagtgggt gttttgaata tatccaagaa ttcaatgaac tgatcaatca gttggaagaa
     4261 atctatgaac taactttgag ccaatatgaa aaagatttta tcagttttcc tttgaacatc
     4321 atcaacacaa gtataacgat gataaggaaa gctaactctg atttgcgctt atatttcgac
     4381 cccatgatga tgcgtattcg acgattagat ctcattgatc tggatgagtc cttcttatat
     4441 caagagatga aacagcacct ctctttattg ataaatcggg taatttttcg ttatcgattg
     4501 actgatttat tttatggtga gatagaaaaa aagtatcctt tttcttacca aatcgctagc
     4561 gaatgcatac aagcattaga agctatcttg caacataaga tctctaaagt agagatcagc
     4621 tacctaacgt tgtatttgga gttagcattt cgatatcaaa aagagcattt ttcctctaaa
     4681 aaagtagcca ttgttcatac gacaggaaaa ggaacagcat taatgatcca aagacaaatc
     4741 aaaaggatat tggggaggga tatacaaata acacatgttt cagaagaaca atatgaaaaa
     4801 gtagatctaa acaaatattt cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa
     4861 gagacaccgg tgatccattt gactaatctt tttgatgact cttggttaag ggcacaatgg
     4921 caaaaagcaa aaaaaaagcg attagccggg tttcattcta ctgttttttt ctatcaaata
     4981 tggaaagaaa aaaagacgta tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga
     5041 ggctattatg tggatgaaga atttgaaaaa aggatcttga aaagagaata tcagcaaagc
     5101 acatttgtta acaaagaatt tgcttttcct cacacaacca accagctatc tcaacacatt
     5161 gttttatcag tagggctaat aactgatcaa ggtttagcag aaacaggagg aaatggagtt
     5221 atttttttat ttgccatacc tgaagaaatg acggctgaag ttgagaatga attatttgag
     5281 ctatacgata tcctatttga actcataaaa atgggaatag atgagtttat tgaattagca
     5341 ccgacacaag aatcattttt aaaactaatc aaaagtaaag gagagagact tcatgaatga
     5401 tatgtatcta ttcatagttt tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg
     5461 gaagcaacta aagcatttca atcaaacata tagcgaactg cgaagaaaag gaaaagtagc
     5521 gattggtaaa cgacctggga aaatccgttc aggcacgatt gttatgctag ctatagacga
     5581 tcatgatcaa gttttagaag tcagaaaaat gcaaggggtt acatctttgg caaaatttca
     5641 ttcattacct gtctatgcgg gtgaagatat ccattacatc gatcgttacc atccctcagt
     5701 tcgagcggaa gatcgtttga cacaagcagc tttagaaaat gccagagaag tatacttacg
     5761 aatagaggca ggaaatgaaa aaagtgaaca accttcattg ccttttattg ctatgctcaa
     5821 taattttcgg atatggaaaa gtacgatcaa agaaaaaagg agtgacaaac gatggatatc
     5881 ataacaaaat ttgctgaggg atttatgagg ctgttccaaa caggtgcaga aacattcata
     5941 agctggatga caggaatcgt accagttgtc ttgatgctga tggttgcaat gaatacgctg
     6001 attgcttttt taggtgaaga ccgagtaacg aaagtagcca aattatcagc gaaaaatccg
     6061 cttactcgtt accttgtgtt accgttctta tctgctttta tgttgggaaa tcctatgtca
     6121 tttacaatgg ctcgtttcct tccagaatac tacaaaccaa gctattatgc tgctcaagct
     6181 caattttgtc atacgagcaa tggtgtattc ccccatatta atcctggaga attgtttgta
     6241 tggatgggga ttgctcaagg gatcgaaacc ttagggctaa attcgatgga gctggctatc
     6301 cgatacttgc ttgtgggact gctgatgaac tttattggtg gttggataac tgattttact
     6361 actggatttg tatgcagaca gcaaggaatc gttcttagca aaaaagtaga gctttcagtt
     6421 gattagagaa aggagagttt taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg
     6481 ggtatggagg tcctttgatt attaggccga caaaagataa acacaagttc atctatgtca
     6541 caggtggagg tgagaggcca atgatcgtag ataaaataga agaactgaca ggtatggagt
     6601 ctatcaatgg gttcaaaaca tcaatacctg atagtgaaat cgcgcttgca atcgttgact
     6661 gtggagggac tcttagatgt ggaatttatc ctaaaaaagg gataccaacc atcaatatcg
     6721 tttccacagg aaaaagtggc ccattagcac aatatatcac tgaagatatt tatgtttcag
     6781 cagtagggtt agaacaaata cagccactaa tggaaactga tgataacaaa gaaacagtaa
     6841 atactgcatc tccagataaa aagtacaaat atgatacttc taaaaagata accgagcaaa
     6901 aagcagagca gcaaaactgg atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta
     6961 cattcaatca atcagcaaga gaagcagtag atacgatgat ccggacagtg atccctttta
     7021 tggctttcgt ttcccttttg attggtgtga tacaaggttc tgggatcggt aatgtatttg
     7081 ctaacattat ggcaccttta gcaggaaatg cagttggatt aacaatcatt ggctttattt
     7141 gttcactgcc atttttatct cctttgcttg gtccaggggc agttattagc caagtgattg
     7201 gaacattgat cggtgtagag attgggaaag gaaatattcc tccccaatta gcattaccgg
     7261 cactatttgc tatcaatact caaaatgcat gtgattttat tccagtcggt ctaggtttgg
     7321 aagaagcaaa agctgagaca gtagaagtag gtgtaccatc agttctttat tcaagatttc
     7381 ttaacggcat cccacgagtt atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAYYNQ010000115         7436 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000115 DAYYNQ010000000
VERSION     DAYYNQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.117"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..370)
                     /locus_tag="ACHBJ1_002563"
     CDS             complement(41..370)
                     /locus_tag="ACHBJ1_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM9431465.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(360..623)
                     /locus_tag="ACHBJ1_002564"
     CDS             complement(360..623)
                     /locus_tag="ACHBJ1_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM9431466.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1083..1886)
                     /locus_tag="ACHBJ1_002565"
     CDS             complement(1083..1886)
                     /locus_tag="ACHBJ1_002565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM9431467.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1864..2988)
                     /locus_tag="ACHBJ1_002566"
     CDS             complement(1864..2988)
                     /locus_tag="ACHBJ1_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431468.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3004..3819)
                     /locus_tag="ACHBJ1_002567"
     CDS             complement(3004..3819)
                     /locus_tag="ACHBJ1_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9431469.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3809..4681)
                     /locus_tag="ACHBJ1_002568"
     CDS             complement(3809..4681)
                     /locus_tag="ACHBJ1_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM9431470.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4766..6055)
                     /locus_tag="ACHBJ1_002569"
     CDS             complement(4766..6055)
                     /locus_tag="ACHBJ1_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM9431471.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6199..6711)
                     /locus_tag="ACHBJ1_002570"
     CDS             complement(6199..6711)
                     /locus_tag="ACHBJ1_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431472.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(6778..6927)
                     /locus_tag="ACHBJ1_002571"
     CDS             complement(6778..6927)
                     /locus_tag="ACHBJ1_002571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9431473.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
BASE COUNT         2286 a         1597 c         1140 g         2413 t
ORIGIN      
        1 atcagtcctt attgacaaag agcccttctt tttagtatta ttataattct tccatgcgtt
       61 cttccagtcg ctgtaaatcg cttggactta attccccaat ttttttccat ttgacctttt
      121 tgttgttttt gggtaattcg tttagagttc ctacgtcaat ccaagattct ttttttagtc
      181 ctgcctgttt ccaatccctt atttcaacgt atttcagctt tattttatcg gatttagtat
      241 gatattttga agtaattgac agaaacgaaa ttgtgtccag tccatttttt agaatataga
      301 caggccggcg tttaccgccc tctgcagctg tgaaagcaac gtagacggtt gctatatcat
      361 tagtattcat cttcattgaa ccattcttca attttttcgt cagtgtctag tttttggaca
      421 ggcaattttt ccgctaatct ttgaatatta tggattcgtt tacttttttc cgacaattca
      481 atagaaaatg ggatttttcc ttgctcaaca atttgcttat aaaaaacggt gacggctgtt
      541 ttggggctca tgcctagttc atcaataatg ctttctgctt catttttcaa tttttcgtcc
      601 atagaaattt gtaacttagc cattttctcc acctctttct gacaaacaca ttatatcacg
      661 gaaagagttc aaatggaatt aatacaacta cttttgaact ccttgaaatg cagtgtattt
      721 atctaaatat aaggaaagga tatagtgcta aatggccttg atagatagaa aagtttcaaa
      781 atccgccaca gcgataattg aagcgaacac tcaacgacac acagaacagg aaaagactcc
      841 taaaagaacc aaattaacta ggacgtgtct gaaaactcca caaagatgtg gtatgctctg
      901 gtaaaaaacg aatggcacaa ccacgaagac cgatatggtt cttggaaaac tttctccacc
      961 ctttttgtaa ttggagggat agtggtttat tgatagcgat tttccaagtt cttcaagtgg
     1021 aaccagacat tgagcaccac ggctaaaaag tgaatgaagt ctttattttt aatcaataat
     1081 ttttaatgat taccagtaat tggttttttc atgcgcccta tttctatttg atgtggagtt
     1141 aaataatgct gataagtaaa aatttttccg tcaattaaac tgccactagt gaatcctgtt
     1201 ctattggtaa aaagagaata gtctgggtat tcatctacac caaatgataa agattcagcc
     1261 ataaactgtt ttttaccagc aaattcagca ctgctacctt gatggatatg tcctacaaaa
     1321 atacctaaaa tatcactttc ggcaatcaat tcagcaaaac cttccggaac ttcagttaca
     1381 atagatggat cttcccacga caatggatga tgaaccaata aaatcgttcc cttttcagca
     1441 ggtgtaccca accactcttg taattgaatc atctgtccta tcgatatttt tccttcgtgc
     1501 tgggtatctt gagcagaatc caaggaaata attcgtaacc cattttgatt cgtacaatgg
     1561 atatactctc ccgtttcatt gctttcaaca ctcattcctt caaagaaaac actgcgacga
     1621 tcatgattcc cacgacaaaa atagtaaggt gtggttggga aatattgatc caacattttc
     1681 cgtaagactt gataatcaga aatcccccct tcatgtacta aatcacctgt taatagaata
     1741 aaatcgtaca ctgttaacga gagttcttgt aacaattttt ccaatgccaa ctgtggattt
     1801 ttaattgttg gaaatagttc agcagtaaac aagttagttt gatagttttc tctaaaatgg
     1861 atatcagaga gatgcaaaaa gcgcatggct attccctctt tcttcaataa aataatcagg
     1921 atatcctaaa ttttcttgcg tctgataatc aaaaaaatgc attctttcag gttctatgcg
     1981 gagattgact gcatcaccaa tctgccaagt aggtttttca ttaaggacaa tcatttctat
     2041 accatcgact ttaacggtgt acgcataact ttgacctaat agctcacgat acttcacttg
     2101 accgtgtaaa tcgcttgttt cttttgaaaa ggttacgtgt tctggtctta tgcctaaata
     2161 caatgagcca ctaacctgaa gaagttgttt ttgccaagct ggattcaatg ttaaaaattg
     2221 actggctacc tttaatgttc cttgatcata agtaactggc ataatattca tgcctggcga
     2281 accaataaaa tttgcagtaa aagtattttt aggtttcgta taaacatttt tcggcgtatc
     2341 aatcatctga attctacctt cattcaataa aacaatccgg tcagctaacg tcatcgcttc
     2401 aatttgatca tgagtgacgt agacaaaagt ttgtttataa cgttgatgaa tttttaccaa
     2461 ttcttttcgt gcatccaatc gtaaacgcac atccaaattt gataacggtt catctaataa
     2521 aaaataatta ctccgtttaa ccaccgcacg agccaatgct acacgctgcc gttgccctcc
     2581 tgacaaatct ttaggaagtc gatcttggta tggcgttaaa cctaacatct tcaaagcttc
     2641 ttccaaacgg tgctgaatgt cagctgtctt aattttattc gcccttaatg caaaaataat
     2701 attctccttt acagtcatgt gaggatataa agcataattt tgaaaaacca ttgcgatctc
     2761 tcgttctcca caaggtagct tatttgctag ttgtgtacct aaaaaaagct ctccactcgt
     2821 aattttttcc aaaccagcaa tcattctcag caaagtagat tttcctgaac cagatggtcc
     2881 cagtaacaca attcgttctc cagcggccac agctaaatca atttctttta aaatggtatg
     2941 ttctccatat ttttttccga tatttcttaa ttcaagcgtt tctcccatga tttgtcctcc
     3001 aatttattta atacctgcag aaacaaaagt cccgataata tatttttgaa aaattgcata
     3061 caagactaac ggtacaaaca ttgtaattaa actaatcgcc attgctacgg taaaatttgt
     3121 cccaccttca gaactaatat aggtctgcat cgccaacggc aaagtataat tatctactgt
     3181 ccgtaaaatt aataccggcc aaacatactc attccaagag gtaataaaaa accaaatacc
     3241 ggtagaagta atcgcatgtc tagtaagagg aaaaacaatt ttactcatga cggttgtttg
     3301 tttgatgtta tccaatgccg ccacatctag aaggctcact ggaatttccc gcattgtttg
     3361 cgtcaataaa aaaatgccca tcgcatcagc aaattgtggt attatcacac cccaagtagt
     3421 atcaattaaa ttaagttttg caatcaacaa ataattagga atcatcgcaa cagtaaaagg
     3481 aatgaacatc gtaccaatga tgataaagta ccagattttc ttccctttga ttcggcaata
     3541 agtaaaacta tatgccgcaa aaaaagctac gcctaactta acaaccgtta taattgtagc
     3601 aataaaaaaa gtatttctcg taattttgat aattggaaga ttttcgaaca aatagcaaaa
     3661 attttccaag gtaaacggct gtggtatcaa actagtaatc gtctgatagg catctttcaa
     3721 tggcttaaaa gaattggcca taataaacaa taatggatag ataataatga ttatgatgaa
     3781 cataaataag agatgtggca accagcgttt aattttcata gtaaaccccc ttttccacaa
     3841 atttaaatat tacccctaag aaaacagcaa atattaataa agttaaaact gaaattgctg
     3901 aagattctcc gactttatac atttcaaaag ccttttgata agcataatag atcacgtttg
     3961 cactaccatt attcggtcca ccacgagtga tgatcttaat cggagtaaac acatactgca
     4021 gaccttgaac aatggtcatc accaataaat atagtcctgt cgaactactc ataggcaaca
     4081 caattgtaaa aaaaatgcgc cagatgggta cacgatccaa cttagctgct tcgatcatct
     4141 cttgatcaat tcccacaatt gctgcaaaaa tgacaatgaa attataacca aaaactttcc
     4201 aagcagtaat acctgcaatc acagcaatta cccaaccttg taaattcatc cacataggca
     4261 gtcttaaacc aaaatttccc aacaagacag caattggtcc cgataccgga tttaatatcc
     4321 atgtaaaaac catccccgcc actaccaaag aaatcacagc tggaataaaa aataatggtt
     4381 tatacaaatt ctgccctttt tctataacta ttcctaataa aaaggatgta atatatggga
     4441 acacacagct cagcaagaca aatagaacgg tataggacaa agtattcttc aacactttat
     4501 gaaagactgg atcagtcagc acctttacat agttggcaaa gccaacaaat tctttagtgg
     4561 gggccaccat attccagtca aaaaaactca aatagatcgt ataaatcaat ggataaaaaa
     4621 caaacacgcc caacaaaata attgctggcg ccagatacaa ataacctaca tagcgcttca
     4681 tttccgacag cctcctcatc cttatgtcaa aagcagacga ccttgttttt cctttcttac
     4741 aggcgctgtc gtctgcttgc tttgcttatt tgagtaactc attaatttcc ttttgagtag
     4801 ctttcatcgt ttcattgacg gaattacctg ataaaatttt atcccgcata tccaacaatt
     4861 tttgttcagc ttctaatcca gcatctccag gaaatgaact ccaactgacc atcttatcca
     4921 tctgatcaat tgctggtatc atcatctgat tttcagctaa gaaagctttt aatccatttt
     4981 gggcgtctgc aacatcttta cgtggtggta aataaccagt accttctgtc catttagcta
     5041 aagattcaac actataaaga tattttaaga aatcccaagt tgcttccttt ttcttatccg
     5101 tttgtgatgt aacagctaac attgcgccac cagcaggtag tttaactttt ttcccttccc
     5161 aacttggtga ctcaactgct ttagcagtaa aagtactatt tttttgaatg ttgtcccttt
     5221 gagcaatagt ggtatagacc atcgcgacgt taccatcaat aaagctttgc acaccttgtt
     5281 cccacgtagt gtgtaatgcc gtctggtctt ttaccatcct ttgatataat tcataagcag
     5341 cgataccatc tttagaagca aaggtagctt tgccatcttt tattacactt gccccattac
     5401 tttctaaaat tgcttgttgt gcccaattat cagcaggttc ttgaatatac acgccatact
     5461 ttccagtttt ttcttttatt atcttagaaa aagtttgaac ttcttcccaa gtttttggac
     5521 cattttcatc caagcccgct gcttttaata tatcttgatt taaatataaa accggattac
     5581 taatggagta aggaataccc acttgtttac catctttttc agacagtgcc atgatattgt
     5641 ctaaaaaatg atctttaaaa taatcagcat tatccggatc cacttcttct actaaagatt
     5701 gaggattagt atatttgaaa ttttctgcaa aataatcttt aaaagcccat cccacttgta
     5761 ctacgtccgg tatttctttg gaggcaacag aagcttgtaa attttgcatc aagcctttat
     5821 acatatcagg attatatttt gctacaacct tcactttgtc ttgagattga ttgtatttat
     5881 ctactaattc attcactgtt tttccaccta gggtatccgc attaacatgc caatactcta
     5941 tcgttacacg accctccgat ttttttgttg tcttggtatt cccactacca caagctgcca
     6001 aagagatgac acttaaaaca gacacaaccc ctataacaag atttttcttt aacataacat
     6061 taacttcctc ctaatttctt tatggccatt tttctaatca agaaatatta tgaactacca
     6121 atgtaaattt ctagttaatt ttctgtaaat aattcgaaat ttgtgcattt cggggtacag
     6181 ggtcactgga catatccatc aagctaagaa taaagccccc atatggtcat gtatcccatc
     6241 tccaaatctc ttaactcctc tgttttgatt ataaaatagt ttattctgtg ctcctttctt
     6301 ccataaatag gcccatgcac aacgtgaggt tatattataa acaccacagc gcgcaaggta
     6361 ctcaatcgtt tctagttcat tgactgtcca tacagaaaat gtcaatccta gctgctgccc
     6421 cacttcacgg gccaacttag ttaaccatcc ttcatgaata ttgacgattt gaacgctttg
     6481 ataggctaat ttctgtaagg tatcgatcaa atctgcttcg gtaatatccc caagtttttt
     6541 cccttcgaac tgattttcaa tattataaaa tatatgcttt ccccaaatcg cagcccaaga
     6601 taattgtaca tagccactaa gaacaatttg tggccagatt tgataggttt gtgccagcga
     6661 aaaaacggcc atttccaatc cctcttcttt taaatcacag ttaatggtca tggttgcctt
     6721 ctcattggcc actgcttgaa acacctgctc aagggttacc gaatctggga caacttctta
     6781 cctgtcatgg gctagtatta gcgtaccatc tgtttgctga cgcacatcta cttcaaaaca
     6841 aagctggcga tccaaacaaa agcgaagaaa tgcccagcta ttctcctccg tattgtcact
     6901 accagagtgt gcagtgatca tggtcatttt gacccctcct ttaattccgt gattttctcc
     6961 cacacgtcca ctataccgat gaagcgttaa tttcttgtta atttcttgta aattccctgc
     7021 gttttttcag taaactagtt gtaaatcttg catgctcctt cctacgaact catgaatcgt
     7081 cgattttgcc cttctagcta gtttcaccaa ttaagtagtt ctacattccc cgcttttaac
     7141 ctactacacc tttttttgtt ttcagctatt ccttcttctt gctgaaagtc cttgaggttc
     7201 tttcttacag gataccatca aggtgttcaa tggcttattc ccattgaatg accgagactt
     7261 actatcagat ttctgctcgt tgaaccaagt ctttgataca ggatttcttc cgtcatcttc
     7321 tcacaaatac caccttgcgt ttctctagcg attggtcgat gtgcaccctc ccagtggact
     7381 gtcactaccc ctttttgcta tgcctagccc acaaaaaaag ctgtcctcac agacag
//
LOCUS       DAYYNQ010000116         7360 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000116 DAYYNQ010000000
VERSION     DAYYNQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.118"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..106)
                     /locus_tag="ACHBJ1_002572"
     CDS             complement(<1..106)
                     /locus_tag="ACHBJ1_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase"
                     /protein_id="HFM9431474.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFV"
     gene            105..953
                     /locus_tag="ACHBJ1_002573"
     CDS             105..953
                     /locus_tag="ACHBJ1_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM9431475.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1599..1805
                     /locus_tag="ACHBJ1_002574"
     CDS             1599..1805
                     /locus_tag="ACHBJ1_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM9431476.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(2007..3008)
                     /locus_tag="ACHBJ1_002575"
     CDS             complement(2007..3008)
                     /locus_tag="ACHBJ1_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM9431477.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(3013..4926)
                     /locus_tag="ACHBJ1_002576"
     CDS             complement(3013..4926)
                     /locus_tag="ACHBJ1_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM9431478.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(5094..5600)
                     /locus_tag="ACHBJ1_002577"
     CDS             complement(5094..5600)
                     /locus_tag="ACHBJ1_002577"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM9431479.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5760..6200
                     /locus_tag="ACHBJ1_002578"
     CDS             5760..6200
                     /locus_tag="ACHBJ1_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM9431480.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(6226..7254)
                     /locus_tag="ACHBJ1_002579"
     CDS             complement(6226..7254)
                     /locus_tag="ACHBJ1_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431481.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(7297..7341)
                     /locus_tag="ACHBJ1_002580"
                     /pseudo
     CDS             complement(7297..7341)
                     /locus_tag="ACHBJ1_002580"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2128 a         1421 c         1138 g         2673 t
ORIGIN      
        1 ttacaaaaat attttttgtt tgattttttc ctactctcct tgtatcttta acattaatga
       61 gaacggaaaa aatgataatc ttattatgaa aatgaggaat tctcatgagc gtctccataa
      121 gactacaaat caaaacaatg tacaacgact tcagcccaaa agaacaggct atcgccgatt
      181 atattttaga aaatccaagc aaagtaagtc atagtcctat tagcaattta gcaagtgaac
      241 tgggcattgc tgactctact ttttttcaat ttacaaaaaa acttggttat aatggattta
      301 aagattttaa aatggccatg ttgatccaag aaaatgatct atctgctatt tctatccacg
      361 aaaatattca gaaaacagat actgagctca ctatggcgca aaaggtgttc gagtctagta
      421 tttcaacttt aaatgacaca aaaaagttgt tacaacaaaa ggatctaaaa aaagcggcta
      481 ctattatcaa tgaatctaaa cgtgtctatt ttttcggtgt tggcggttca gagattgtag
      541 caactgacgc ttatcataaa tttttgcgtt cccctatcgc aacttctcac agcacggatt
      601 accatattca attgatggaa gcttctttat tgacggaaaa agactgtgct gttttgattt
      661 ctcacacagg gcaatcaaaa gaaacaatcc atattgctga aaccgtaaga aaaactggag
      721 caaaaacaat cgttattact agccaagcta attcttccct tgctaaactt ggcgatgtcg
      781 tttttatttc tatttctgaa gaaacagaat tccgatctga agctttagct tcccgtattt
      841 ctcaactaag cattttggac tctctatatg tgattttgat gttttacaac gaaaaagaat
      901 cacgtcgttc tatcgctaaa gttcgacata ctatttctga aatcaaagaa tagcaccata
      961 acagaaaaag cgaaaaatcc ataaaacaaa aaaagccact accaaatatt tggtagtggc
     1021 ttttttggtt ctgcaagggt ggtacagaac atcaatagtc ggtgggttag aacgtatgtc
     1081 atcgcatctt tggagaaggt tgtattggta ttattcgagt tacttgttag atgcgataac
     1141 aaagttagta tacagtagta aaaaatttat cttttctttt ttttattatt tttgtttctt
     1201 attttctaaa aaattaaaaa aaattttggt gaattaagta tttccttgat aacactggct
     1261 tttggcctca ttttaggtta tcatttgtaa ttttatttca cagttgatat gattgttatg
     1321 taaaataaaa aagtttagga tacatcgaat ttttggggaa attcttttgg gggtatccta
     1381 aatcttttct attatattcc catattttct attattggtc tagacgtatg atggtaaact
     1441 ttgtacctat ctgattgctg aatcattaat tgcttatcat tagccatctt tgtttcaagt
     1501 tcgctagatg atcattttat tcacatccta acaaacttgt cacatttata gattcttaat
     1561 ctatttatca tacaattata aaaaaggagg tgctgcttat gaaagctatc catatttctt
     1621 tgcttttttt cgctagtgca ttgattttat ttgctattca tctttgttcc cagccttata
     1681 tcaataacaa cggaatatta gtggaaccag cattcttttg cttaccactc ggctttttgt
     1741 cccttcttgc ctccgcaggc ttcggatttg ttgctttttt taaacgatca aaacaacaaa
     1801 tctgacttat aaaatttacg taaaagagat cgtgacaata atcgtgtgat attcccaaat
     1861 ttcgaataaa aggtgcccct tgccatctct aactcctcag tcacaagtta caatattcat
     1921 aaaccatgaa aagcttgttt attgaaattg ctccttgtga cttggagcta agtgacagat
     1981 tacaacctct tttgcatcat aagactttac tccttgaaaa acattactaa cttttgatat
     2041 gtttcttgcg cttgtttatt gctgaaatca aactgatatt catggttcaa aacagcattt
     2101 ttctccgcat aatctacttt tgtgactttc acccccgctt tctcaaatac agaagttact
     2161 tcttccattt gtttcgtaaa cgtatttgta tttccatccg ttaagaatgt gttttgcgtc
     2221 caacgatccg catgtctgac caaggctaaa cttttgacct tctctgattg ttcaaagtcc
     2281 ttattatcaa agtaagccca tccacatttt tcataaagaa agctagagat tttatcactt
     2341 acttgagtaa gtgcagaagg atccaacaga ccagacaaag aaataaactt cgtaatctgc
     2401 ttgttctcca aagtcggatc aagatgatca gcttttttaa tttcttcatt ttgaagtgct
     2461 aacaaatatt ctccactaat ttgtgcacct gccgaatttc caccaatcga aatattcgac
     2521 caatctgctt gatcttttat ctctttttta gctgcttgaa tggcttcgtt caattggata
     2581 actggtgtag gatgttttgc ttccggtgat cgttcataat ctatatttac gatgatttgc
     2641 tgcgtgtctg aacatagata ttccaaataa tcacgacaat catttttatc ccctcctaca
     2701 tatgcgcctc catgcatcca gaaaagaatc ggtaattttt tcttttccac tttcggtgtg
     2761 tagatatcca tattattatt tttgtacttt gaaggatagg atatttcttt ttttatatcg
     2821 atttgttcga ttttttgctg gaaatcatct ggtcgggcat atacttgttc tttttcactt
     2881 gtgtcaaatt gttttctaac aagcaagcta atcggctttg gtgtgatttg attaatcaaa
     2941 aatacagtga ctaaaataac tacgatgata actggtactg caatcactat tcgcttgaac
     3001 catttcattc atttaccctc tctttcttcg ccaaatccat gtgataatcg tgctaacaga
     3061 taaaaatcca cctgaataaa tgagaagcga atgtttcttt tctccagttt tcggcaacat
     3121 tctttttttt gcatctgtct ttttttgtac tttcactgtc atctgaatcg gttgcgtatc
     3181 atcacttgat tgatcctctc gaggttcatt ctttccctta ttgtctgcat tctctgtcgg
     3241 atcttgcttt tttggtggag ctactgggat ttttttgtaa tagaaaataa tagttggtat
     3301 cgtctcatta aaaatccctt cagtcggtcc ttcaacattt gctaattcgt atccttcgaa
     3361 tgtcttttgc tctgtcatat aattttctcc ataagctccg taaagctgga tttcgtcctg
     3421 gtctttgagt agacttttat tgttttcatc cacatacttt acgtttatct gatcatggaa
     3481 cactactttc aaaaagtccg ttacatgttt cgtgtaggca gcatggtcaa cttcctgtgc
     3541 ctttccatgc cctgctcctt ctactaataa aagatcttta taccctcctg ataactcata
     3601 taatttttgc gcgttagaca caggtactac atcatctgct gttccatgga taatcaattt
     3661 aggcaatgaa tctgacattc caatcgtagg tagagcagct tttgtatcca aatgcatctc
     3721 ctgatcaaaa tagtattgtt tcaaaaggcc cataaataaa tcttcatgct cttgatcccc
     3781 aatgatgtct aactgatttt ttatggcatc cggtagtgcg ggggctactg tctggctgta
     3841 cgtaaaacgt agttgttcat caattgatgc aaatccgcaa ttttcaataa ttcctttgac
     3901 attgacagga atatctttcg ctaatacaga catcatcgta gctgctccca tcgaaccacc
     3961 ataaagtaaa atcttttgag atggccgctc tcgaacttct tgatttatcc agtaaaggac
     4021 gtcatctgac tcatattgtc cataggtcac ataatcgcct ccgctattac cagtcgctct
     4081 ggaatcaaca aataataaat tatacccggc cttataaaaa tcattgtatt cttctgtaac
     4141 gatcccgtta ttccaattcc ctcgaaatcc gccatggatc atgactgttt tatctgaacc
     4201 ttgatcgaca taatatgcat atacatccat cattttgcca tcttgtttgt ttagtacagt
     4261 ttgatgaagt tgttttgtat ttggatagaa gcctgtgttt tctgtaggcg ttgtcccatc
     4321 atctgtacct ccaagcatcg acatcaacac actgtttttt gcaaaagcga tatcgtacaa
     4381 tagataagta gcaatcagat gtttgataat ctctttatct acaccataat cattaacagc
     4441 aagctcagtg actaataact ctattaattt tgacttgatg tcttctgttg gtccattaag
     4501 tatttctgtc atctgtttac cgaagtaatt caaatattct ttaccattga aattttcacc
     4561 tttaccgata tcatcatttt ctgtcccatt ctctttgtca cttactttag cagaagagga
     4621 ttcgtcgttt gacatttttg tagctgttgc ttcttctgtt tgttggttat ctgtggtagc
     4681 ggtcgtttct tgatctgtgc tagtaacaga tgtctcaacc gttgtttcag tatttgtttc
     4741 tgtttgtgaa gtactcgatt tcgtttcatt tccagcatca gtagtttctg tggtttctac
     4801 ctcccgagat tccgattcgg aagaaatgct ctctccatat actgtccatt gcgggacgct
     4861 tgtacttaca gcaaataaga tgaccaacgc tccctttaat acttgttttg ttctcaagct
     4921 attcactcct ttttatgttt ctaggagaat tatatgtaaa cgcaaacatt ttttatacaa
     4981 aaaataaatg gtttatccca taaaaataga acaggattat tttctcattt tacgtaataa
     5041 agcagaatgg ataaaccagt atccattctg ctttttttaa acgaagaaac aacttattgc
     5101 tccaaaaatg tcagaaatcg tttatcccaa attttctcgt aaaaagcgat tgtttccttt
     5161 gctttcataa aaggattatc ataagttgtc gttgctcctc tacacatcat aagctggtat
     5221 cctaatccat gagcaaactt cacagtcgta tctatacaat actctgcttg tgccccacat
     5281 aattcgatcg aatgacagtc caattcagtt aataaaacac tcaaattagt cttataaaat
     5341 gaattcgcat gggtttttct cacaaaataa ctgtgctcag gaatagataa ttccgacaat
     5401 agctcccatg gttttgtttg tggcactaat tgagcatctt cgtgttgaac gaaaataact
     5461 ggtttcccaa tcgtttcgta tgttttgatt cttttattca ctaatgctgt cactgtttct
     5521 aattcgtgta tcggcgaaga atcaaaacat accccttttt gtaaatcgat cactactatt
     5581 gcgtcggctg tgtttgacat acttgtgcct tctttctttt ttcttgattt aatagtattt
     5641 cttatctagc tagttccatt atacgtgaca acattttctt attttacacc tctaaatggc
     5701 acttttcgac tatttcttca tgttattatt tcataaatac ttagctattg gaggaatgta
     5761 tgatgattaa attgtataca ggttctggct gctcgtcttc aagacgcgca aaacgttggc
     5821 ttatcgtcca tgatataccg tttatcgaac agaacttaat tcgtcaacca ctaaccaaaa
     5881 atgaatttat ccacttacta aaattgacaa acaacggcgt gaccgaaatg ctctctactc
     5941 attctattag ttataaaaat cttgttcaca gaatagatga actcagtttg agtgaactat
     6001 atattgagat tcgtacatcc ccttctttat tgaaaaaacc gattatctac gaccaatctg
     6061 caaaactttt gactggattt agcgaagata aaatcaggat gttcctttct aaaactaaaa
     6121 gaaagcaaga gctcaacgaa ctcttgcttt ctggtcatct tgtcgatttt aaagaaaata
     6181 atctttttgc aatgtcttaa ttttggcata aacaatctgc ttttcttaat taacgaaatc
     6241 caatttttcc aatatggatc tatttttctc attcctgatt tttatgattt cagctttcag
     6301 acgcgcccaa tcattttttg taggttgtcc attccagtaa aatatctgtg tattcgtttg
     6361 atcatatgcc tttttgtcga taggataatc gctaatgaca atatcaggta actgatcagc
     6421 ggatgcacta tattttattg ggcgttcagt catagataat atccttttct ttgcccataa
     6481 ttcctgtgtt ttaccatact ttgattgaat atagaatatg acaggttgtt cgtgttttat
     6541 aaaaatatcc ctgactaaca tacagtattg aaaaatcaac cgtctgttct tttcataata
     6601 agtattaagc tctggaattc tgcgagctac cccactaaaa aactgttgca tgattggata
     6661 catcactgga aatatatgct gaaaatctct gtcttctgct gattttccaa aagcatccac
     6721 cttctttttt gtcccaaaaa cccttgaata caaatggatc attgccaaat tgacatatag
     6781 ataatttttt tctgaagttg taaaacaaat cttgaagttt ttttcaaaat tttctacaat
     6841 agacgattca atccagcccc aactttcttg tactttatct ggagtatcca acaaatcttc
     6901 ttccaaatag gtgttcatta caccaaacat aaaataaagg taagcttgct ctgttggagt
     6961 gatgtgattc atcgaccaga agtttgcttc aaaaaaaggc tctagtatgt aatgcgtaaa
     7021 ctcttctttt gttatcaatg gatgcacaaa tttgttcatc tcttctggga catacgtaat
     7081 cgagtggcct tgtttcattc gttgcaacga aattcttagt aggctgatcc atttctttct
     7141 atctatacta cgcgtatatg gcgcatgttt cgcaaatttt tctaatagtt gacgtttatt
     7201 ttttatactt gataatatat cagaatgatt ttcgtcgaat gactgccaat acatcaagtg
     7261 aaaaaagtat cggatacggt attcttctcc ttctatcgga ttaccttttt tcaagctgca
     7321 agataaatga tagtttttca aaatagtatt aagttctctt
//
LOCUS       DAYYNQ010000117         7139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000117 DAYYNQ010000000
VERSION     DAYYNQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.119"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..615
                     /locus_tag="ACHBJ1_002581"
     CDS             <1..615
                     /locus_tag="ACHBJ1_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9431482.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="ACHBJ1_002582"
     CDS             612..1385
                     /locus_tag="ACHBJ1_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM9431483.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="ACHBJ1_002583"
     CDS             1382..1780
                     /locus_tag="ACHBJ1_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM9431484.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="ACHBJ1_002584"
     CDS             1965..2636
                     /locus_tag="ACHBJ1_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431485.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="ACHBJ1_002585"
     CDS             2695..3135
                     /locus_tag="ACHBJ1_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431486.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="ACHBJ1_002586"
     CDS             3147..3815
                     /locus_tag="ACHBJ1_002586"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM9431487.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="ACHBJ1_002587"
     CDS             3879..4502
                     /locus_tag="ACHBJ1_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM9431488.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="ACHBJ1_002588"
     CDS             4558..4944
                     /locus_tag="ACHBJ1_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM9431489.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="ACHBJ1_002589"
     CDS             5157..6548
                     /locus_tag="ACHBJ1_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM9431490.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2276 a         1160 c         1572 g         2131 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacag
//
LOCUS       DAYYNQ010000118         7138 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000118 DAYYNQ010000000
VERSION     DAYYNQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.120"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..414
                     /locus_tag="ACHBJ1_002590"
     CDS             58..414
                     /locus_tag="ACHBJ1_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9431491.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            404..670
                     /locus_tag="ACHBJ1_002591"
     CDS             404..670
                     /locus_tag="ACHBJ1_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9431492.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            829..1830
                     /locus_tag="ACHBJ1_002592"
     CDS             829..1830
                     /locus_tag="ACHBJ1_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM9431493.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(2200..2814)
                     /locus_tag="ACHBJ1_002593"
                     /pseudo
     CDS             complement(2200..2814)
                     /locus_tag="ACHBJ1_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3054..3299
                     /locus_tag="ACHBJ1_002594"
     CDS             3054..3299
                     /locus_tag="ACHBJ1_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431494.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            3397..3618
                     /locus_tag="ACHBJ1_002595"
     CDS             3397..3618
                     /locus_tag="ACHBJ1_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431495.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            3940..4620
                     /locus_tag="ACHBJ1_002596"
                     /pseudo
     CDS             3940..4620
                     /locus_tag="ACHBJ1_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            4760..5299
                     /locus_tag="ACHBJ1_002597"
     CDS             4760..5299
                     /locus_tag="ACHBJ1_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431496.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            6319..>7138
                     /locus_tag="ACHBJ1_002598"
     CDS             6319..>7138
                     /locus_tag="ACHBJ1_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9431497.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT         2611 a          979 c         1261 g         2287 t
ORIGIN      
        1 attcgaattt ttatgttata tactataata taaaatcaat atatgatata attgggtatg
       61 gaaaaaatat cttttgaaac atttaaaaga cctaatggtc atgatgaatt tttagaatgg
      121 ttggaaacat taccaaaaaa agatagtgcg aagcttttac gtacaataga agaaacagaa
      181 aagaatggga tgctagtagc tcaacggttg aaatgggtga aaaaattaga cactaatttg
      241 tacgaattaa gaagtaaggt aggatcaaac atccaaagag ctatctattt ccatgtggtc
      301 aatggaaggt atgtcattac ccatggattt actaaaaaaa cggagaaaac accaataagt
      361 gaacttaaac atgcaaaaga acttagagag gagtggttca caaatgatga ttaaaaaatt
      421 gattgaagaa aagcaaaaag acaaagattt tgcagaagca tatgaccgtg aaggaagaaa
      481 actcgaaact gcggttgctc tttatcatgc acgggaggaa gcaggcctaa ctcaagcgga
      541 gcttgcacaa aaagcacata caactcaatc tactattgct cgaattgaga gaggggataa
      601 cgtatctttc gataaacttg atgatatcgc tagggcatta ggcaaaaagc tggtagtaga
      661 atttgcttaa aagcgagttg aaaatatacc tgtaataggc tcctaactaa tcaattagtt
      721 tagtgggcct ttttgtacta aatagataaa agttgtccta tagtgtaaaa gtcaataaat
      781 tttatacaaa aattacttta aagagacata aagagaaagg tacaataaat gaaaaccata
      841 aatctaataa aagtatttat agcatcacca ggtgatatag cagcaaagag gaatgaagta
      901 gaagaaacca tatatagatg gaatatagaa aatacagata atacaaaagt aattttgatg
      961 ccaattcgat gggaaacgaa tgcgattcct acttatataa ataatagctc tgggcaaagt
     1021 gttattaata aacaaattgt agaaaatagt gatatattat tagcgatttt tggccataag
     1081 ttgggttctc cagttgataa ttatagatcg ggaactatag cagaaattga ttacttttat
     1141 aaaaatagca aaggcgaggt aggggttttt ttcttagatg aaccagttcc acaagttttg
     1201 gctgaagagt atatagaagt taagaagtac agggactctt tagaagataa gggtttttat
     1261 ggaacatata gcaaagacaa tatcagaagg tttctcacaa aaaaagtgaa ttatttaatt
     1321 gagaaagcac aaaataattt agaatcaaaa cgctttgatt tagattctat ttcttacaag
     1381 acaccttcag taattaacaa aaaagttacc ggaactgata aagattttga tgacaactta
     1441 gatatttttg atccaataga atttgacaat gatgaaagat tgctgctaat atatgcgttt
     1501 atggatgaaa attctatctt ttcaactgat aattcaactt atagaaaagt agttcaatgg
     1561 gaaagtgata acaatttaag gagttacttg agtgatagag tatttgatgt actagaaaaa
     1621 ttaagtaaat cgggaatttt aaaggaagaa tacgattctt cttttgatga gtatccatca
     1681 cgctatattt tgatttcaga ggactataag aaatttaaaa ggtatttttt aaaaaatgaa
     1741 aaaaagatat ctgttattat ggagaaatac aaagctccaa tacaggctga tccattttcc
     1801 aatttggatg aatcagattt accgttttaa attttaaata gataatttca ttttttagta
     1861 gcagaggtat aaactttttt aatgtttgaa ttgaaaagaa atgctaatag gtcgacttta
     1921 tgtttctctt gtgcaatcgt aaatgcgggg gaccataaaa ttgatctatt atcgagtgtg
     1981 tataaaataa aattcaaatc ttattagttt acataagata cattatacta agttgtcaat
     2041 gttaaatgcc tttaaataag gacgatttat gcatcaatta tatttatgtt aactaaattc
     2101 tttttaacat aaatgtaatc aacttagcta gatgaatcta aaatgattgt ctgtattgat
     2161 gacaaagcta attaataatt acgtgattca aaaaagaaat catacaatta gttggtcgat
     2221 ttcttttttt attcggtaaa ttgtttgcct agtcacattg tagtctgttg caatctttga
     2281 gatagcaacc ccattctcaa gatcctgaac gatactccta tagacaaggc gacgttgagg
     2341 atctttggtg tctgcactgt ataacttagg acgaccttta tagacgccgt ttgctttagc
     2401 gattttaatt ccttgtgcct ggcgtcgttt cgattcagta cgttcttgtt cagcaatcat
     2461 ggctgagatt taaatgatta agtctttgat gaatttatct aggagaggat ttccaattgc
     2521 ttcagccatg atcggtaaac tggtgataat aagtcgcaca ttcttcttct ttagataatt
     2581 cactgaatcg ataatctcgt cataattgcg tccaagtcga tcaatggctt ctacaataaa
     2641 aatatcttgc tctctgacaa aatctaaagc ttcctggaaa atcggccgat gactaatcgt
     2701 cgctccagat tgtttttcga taaaaatttt ttcagcgcca aactttttca tttcttcaat
     2761 ttgacgttgt tcattttgat cgatggatga aactctaata taagcaattt tcaaatttat
     2821 tccctacttt tctataacgt ttataataca ccctaatcat acgttagaac tctaaatttt
     2881 tcaatgttat atttttgtct atttagggta aactctaaat agacaaacgg tttttgaatt
     2941 ctaaaagtta aatagtttat aatagaagaa aaagaatggg gtgacgattt cttagattga
     3001 aaaggaaaga taaatattat agattattac ttttgacagc tgaagcagga tggatggatt
     3061 ttccagaagt tgtaaaagaa gttcaagaga taggtgcaac tatttctgat agccgctcct
     3121 ctcctatgaa ctatcaggat gcccatggaa ttaaaataat tgatcaagag agaaatgtca
     3181 gaaaatacac aacaataaat gagtgtgtaa ttaatgaaaa tctttgtagg gcaccgataa
     3241 cgaaacacat taagaataga agcaaagcaa aagacggaag aacttttact accttctaag
     3301 tttttttcat atttgtttgc tatacttaga tctaaggaat tagactttcg ctgcccatta
     3361 tttaagtttg aggtagcgtc ttgcgaggga tcaataatgg aagaagaaaa aatatttgaa
     3421 aaacgctggg aattggcttc agttgaacaa agagcaaggt accataatct aatgtcctct
     3481 taccgtaata ttgattggac ttacaaagaa aagaagtact tgctttggtt atgtcagtta
     3541 gatgtaaata cctttgaaac atttgaagtg attctagata agattaaaaa cagcaatgaa
     3601 aaaagagctg atctataatg gttcgctctt tttattttat taactctttg gttactatat
     3661 gtaaaataca gcttcatatt acaataaatt tcaggataca aatttattta agcatatata
     3721 aatataattg aaataggaca taaatatatg agtatgagaa aggtgattcc atgtttaata
     3781 aatgggcaaa ccacccaatg aactcgagag gtagagataa tttgaaggag acggacaagt
     3841 tctgggaaga agtatatagt aaaaacggtt ctgttgcaaa gttttccaaa aaatctattt
     3901 tagtgtaaaa ttgagaaaaa agacagagag gacagagtaa tgaatcattt taaaggcaaa
     3961 caattcaaaa aagacgtcat tattgtcgct gttggttact acctgcgtta caatctaagc
     4021 tatcgtgaag ttcaggaatt gttatatgat cgtggaataa atgtttgtca tactacgatt
     4081 tatcgttggg tataagagta cagcaaagtc ctctattatc tttggaagaa gaaaaataga
     4141 caatccttct attcatggaa aatggacgaa acctatatca aaattaaggg acgttggcat
     4201 tatctttatc gtgcaattga tgcggacggc ttaaccttag atatctggtt acgaaagaaa
     4261 cgggatacgc aagcagccta tgctttctta aaacgactcc ataaacagtt tggtgagccg
     4321 aaagcaattg tgaccgataa agcaccttct cttggctccg cctttagaaa gttacagagt
     4381 gtgggtttat atactaagac agagcaccga actgtgaagt atcttaacaa tttaatagaa
     4441 caagaccatc gacctattaa acgacgaaat aaattttatc aaagtctccg tacagcctct
     4501 tccacgatta agggcatgga gaccattcga ggaatatata aaaagaaccg aagaaatgga
     4561 acgctcttcg gcttttcggt gtctactgaa atcaaggtat taatgggaat aacagcctaa
     4621 gatatttgga gttcagagag ggcgcgtttg attttcaaac ttcgcaacag aaccgagaac
     4681 cttgggtttt caagtgcttt ttggttcatt aatcttaacc atagtgtttt taaagtggaa
     4741 aacaagcaac aaaaaaagta tgtggagcct tgtaggaata gttcttatta tttttactgt
     4801 gactgcagca acagaagcaa attattcgca taaaattcag tcgtataaag gtcgtttagt
     4861 tatgaccaaa aaagatgtta cacgttatac atcctcaaga actggaggcc tatatgacta
     4921 tgcaccatta gatgcattga aatataaaga tcatttaaag aaacatgaag taaaagttgg
     4981 tgatcggtgg gagaatgcat ctcataaagc atataattcc aaaatagtct atacagtaga
     5041 agatgccgat gagcaaaata tcacattacc tgtattcaat tattggggaa cagttgtgaa
     5101 agtaaatggg aaaaagagca ctatagaaaa tgacaatggt ttagtgtcta ttaaaggtac
     5161 ggggaaaaga actatagttg aggtttcatc tacgtatcca actacgatga ttctatcttt
     5221 attaattagt tcaataactt ttatattctt atttacacgt tatttaataa gatgctgcgg
     5281 tgaaatgaaa atagattaga tagatattat aaatatgttg attctatttt catagcaatg
     5341 gagaaaatac attagcaata tataaaagaa ggtatcaaga gtcacagtga atttattccg
     5401 aaaagtgaaa agagcaaata taaaatttaa tgaaaatata gaactaaata ggaaaacgta
     5461 gacgtaattt tctacgtttt ccttttcata tatattaaat attacggaaa attatataaa
     5521 ctaatttagt gttttgtttg taattcctaa ttttgctcca cttttagata atttttaggt
     5581 ctatttaact catcagacat tctatgctca tttttctcca aatttgtttc aactctttcg
     5641 tacttaattt tgcgtatttg aaaattcttc ttttagagta cttttattac gatactagta
     5701 tgatatatat ctatagcaaa cagttaaaat cttcctaaaa taagcgaata gcattcctaa
     5761 aacctataaa atttttagat gaagcgacaa gtggacaatt aataaactac ttgaacatga
     5821 aattatggat cacctttcaa atttagaaaa tggaattctt atacttattg cgcaacattt
     5881 attgattgca aaattatgtg acaaaaaaaa ctagtagaag aaagaataat ggcttgataa
     5941 taaagtacta cctattaaag gtagtacttt atttaattta gtataaatta acatactact
     6001 atattttagg tttcatatta tataaattaa taatatacta ccatttgttg gtagtataat
     6061 ttaaaggtaa taagttattc ttttattact atgtttgggt tttatagcaa agaaagttta
     6121 ataagagttt ggacctagca aacaatatta atgaggtcat taaatcttat tatctttagt
     6181 gatataacga aatatctaca cagacaaaat gatagcgtaa aatattttgt gtaaatagat
     6241 atttagggta gaaaaaaaga aagtccattc tgtaaaatta aagttaccac aaccgaaatt
     6301 ttataggagg actttctcat gcccaatttt actacagaaa ttatggaaac actaatcaat
     6361 aaaggtgatt tagatgaatt atttcgatgt catttagaat tagctgttaa ttcattattg
     6421 caggctgaat taacagcttt tcttgattat gaaaaatatg atcgtgctgg gttcaattca
     6481 ggtaattccc gtaatgggaa ttattcacgt tcattcagaa cagagtatgg agaattaaat
     6541 ttggtaattc ctagagatag aaatggtaag ttttctcaac aaactttacc tgcttataaa
     6601 agaaccaatg actctttaga aaccactatt attcagctat ttcaaaaagg aattactatg
     6661 tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc actatacgcc acaaaccatt
     6721 tcaaatatga ctaaaatagt ttctgaagat attattgcct ttaaagaaag gtccttagaa
     6781 tccagatact ctgtcatttt tatggatgct actcacattc ctttaaaaag acaaactgtt
     6841 tcaaaagaag cagtttatat tgtaattggc attcgattag atggaaccaa agaggttctt
     6901 gggtttagtc ttgctccaac tgaatctgct tatgtttgga aagagattct tcaagaccta
     6961 aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg atggtttaag tggcattaac
     7021 gatagtattc atagtgttta tccaaatgct caatttcaac aatgttgtgt ccatatatct
     7081 agaaatatcg ctcataaggt tcgtgttagt gatcgacaag aaatctgtag cgatttca
//
LOCUS       DAYYNQ010000119         7022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000119 DAYYNQ010000000
VERSION     DAYYNQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.121"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..621)
                     /locus_tag="ACHBJ1_002599"
     CDS             complement(163..621)
                     /locus_tag="ACHBJ1_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM9431498.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(634..1965)
                     /locus_tag="ACHBJ1_002600"
     CDS             complement(634..1965)
                     /locus_tag="ACHBJ1_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM9431499.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1976..3418)
                     /locus_tag="ACHBJ1_002601"
     CDS             complement(1976..3418)
                     /locus_tag="ACHBJ1_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM9431500.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3405..4169)
                     /locus_tag="ACHBJ1_002602"
     CDS             complement(3405..4169)
                     /locus_tag="ACHBJ1_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM9431501.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4273..5148)
                     /locus_tag="ACHBJ1_002603"
     CDS             complement(4273..5148)
                     /locus_tag="ACHBJ1_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9431502.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5403..5487)
                     /locus_tag="ACHBJ1_002604"
     tRNA            complement(5403..5487)
                     /locus_tag="ACHBJ1_002604"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5451..5453),aa:Leu,seq:caa)
     gene            complement(5505..5576)
                     /locus_tag="ACHBJ1_002605"
     tRNA            complement(5505..5576)
                     /locus_tag="ACHBJ1_002605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5542..5544),aa:Gln,seq:ttg)
     gene            complement(5586..5658)
                     /locus_tag="ACHBJ1_002606"
     tRNA            complement(5586..5658)
                     /locus_tag="ACHBJ1_002606"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5623..5625),aa:His,seq:gtg)
     gene            complement(5720..5804)
                     /locus_tag="ACHBJ1_002607"
     tRNA            complement(5720..5804)
                     /locus_tag="ACHBJ1_002607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5768..5770),aa:Leu,seq:caa)
     gene            complement(5825..5895)
                     /locus_tag="ACHBJ1_002608"
     tRNA            complement(5825..5895)
                     /locus_tag="ACHBJ1_002608"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5861..5863),aa:Cys,seq:gca)
     gene            complement(5941..6012)
                     /locus_tag="ACHBJ1_002609"
     tRNA            complement(5941..6012)
                     /locus_tag="ACHBJ1_002609"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5978..5980),aa:Gln,seq:ttg)
     gene            complement(6024..6096)
                     /locus_tag="ACHBJ1_002610"
     tRNA            complement(6024..6096)
                     /locus_tag="ACHBJ1_002610"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6061..6063),aa:His,seq:gtg)
     gene            complement(6099..6172)
                     /locus_tag="ACHBJ1_002611"
     tRNA            complement(6099..6172)
                     /locus_tag="ACHBJ1_002611"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6138..6140),aa:Trp,seq:cca)
     gene            complement(6179..6259)
                     /locus_tag="ACHBJ1_002612"
     tRNA            complement(6179..6259)
                     /locus_tag="ACHBJ1_002612"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6223..6225),aa:Tyr,seq:gta)
     gene            complement(6261..6336)
                     /locus_tag="ACHBJ1_002613"
     tRNA            complement(6261..6336)
                     /locus_tag="ACHBJ1_002613"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6301..6303),aa:Phe,seq:gaa)
     gene            complement(6372..6444)
                     /locus_tag="ACHBJ1_002614"
     tRNA            complement(6372..6444)
                     /locus_tag="ACHBJ1_002614"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6409..6411),aa:Asp,seq:gtc)
     gene            complement(6454..6526)
                     /locus_tag="ACHBJ1_002615"
     tRNA            complement(6454..6526)
                     /locus_tag="ACHBJ1_002615"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6491..6493),aa:Val,seq:tac)
     gene            complement(6542..6613)
                     /locus_tag="ACHBJ1_002616"
     tRNA            complement(6542..6613)
                     /locus_tag="ACHBJ1_002616"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6578..6580),aa:Glu,seq:ttc)
     gene            complement(6628..6717)
                     /locus_tag="ACHBJ1_002617"
     tRNA            complement(6628..6717)
                     /locus_tag="ACHBJ1_002617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6681..6683),aa:Ser,seq:gga)
     gene            complement(6757..6830)
                     /locus_tag="ACHBJ1_002618"
     tRNA            complement(6757..6830)
                     /locus_tag="ACHBJ1_002618"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6794..6796),aa:Asn,seq:gtt)
     gene            complement(6840..6955)
                     /gene="rrf"
                     /locus_tag="ACHBJ1_002619"
     rRNA            complement(6840..6955)
                     /gene="rrf"
                     /locus_tag="ACHBJ1_002619"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2119 a         1551 c         1149 g         2203 t
ORIGIN      
        1 agaatcctga aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca
       61 aaaatcaaaa tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat
      121 tcacccataa atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact
      181 atctttaatc tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga
      241 aactacaatg atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc
      301 taacagtgtc ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc
      361 ttcgagttca actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc
      421 gatcgttatg gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg
      481 gatatatcca tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac
      541 ctcgtctttt actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt
      601 tcttgtttta aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt
      661 gtttactttg atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg
      721 attcaatgta aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag
      781 taacttctct actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt
      841 tcttagtgta attggctcca caccagttgc accaacatat gccggtattt caacaactgc
      901 atctgaccgt agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg
      961 atcatcattc aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt
     1021 aaagttcaat acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt
     1081 tttctctcgt ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac
     1141 gaccatatca ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt
     1201 tttcgtgatg actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa
     1261 atcggcgact tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg
     1321 ttgcaattct ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt
     1381 aacgatccaa ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat
     1441 agtcatatca caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga
     1501 aacgatcgac tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat
     1561 atgatcgaca atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag
     1621 tccacacgtt tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg
     1681 catctccaat cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag
     1741 ctttggatca tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa
     1801 tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc
     1861 agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt
     1921 actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt
     1981 cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt
     2041 gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt
     2101 cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat
     2161 ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat
     2221 ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct
     2281 tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt
     2341 aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc
     2401 gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga
     2461 ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag
     2521 gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc
     2581 ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat
     2641 agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa
     2701 acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct
     2761 tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca
     2821 gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca
     2881 tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc
     2941 ccaaaaaaac agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc
     3001 ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg
     3061 ccgctactaa accggaaata attccaccaa aaatccccat attcacttct gggtccataa
     3121 tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt
     3181 tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc
     3241 caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata
     3301 aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa
     3361 gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat atcctcatat
     3421 gttactaact caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa
     3481 aattcgactt ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc
     3541 aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa
     3601 cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct
     3661 acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag
     3721 ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca
     3781 tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa
     3841 attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga
     3901 tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg
     3961 aggagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc
     4021 tttcttagtt tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc
     4081 aaaattcctc gctgataagc atccataatc ccataattca caccatggct atagccaaaa
     4141 tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag
     4201 gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt
     4261 tacgccctta ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc
     4321 ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata
     4381 cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc
     4441 ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag
     4501 tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt
     4561 catttctact atttttaagc tctttaaact attaggctct cctagctcag cattaatgat
     4621 atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat
     4681 tcgctcgacc acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg
     4741 caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg
     4801 atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag
     4861 ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact
     4921 gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc
     4981 aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat
     5041 tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa
     5101 ttcacctcct ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt
     5161 ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc
     5221 ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg
     5281 ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga tttggaggta
     5341 attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat
     5401 tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag
     5461 cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt
     5521 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa
     5581 taaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa
     5641 ccacttcgcc acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag
     5701 atgaagacat cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca
     5761 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt
     5821 actaaggcgg taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca
     5881 cttggctata ccgccattat actattatca atctataaaa taaagaataa attatagaaa
     5941 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
     6001 ggctataccc caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc
     6061 cacaatccgg tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga
     6121 acccacacta acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg
     6181 gaggagagtg gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta
     6241 gccacttcgc tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc
     6301 ttcaatccgt tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat
     6361 aacaagaacg acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca
     6421 tgttaacccc tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga
     6481 ccctctgctt gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa
     6541 atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct
     6601 tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt
     6661 ttggcaccat ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga
     6721 gtgaaagaag ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta
     6781 cgacatcatg attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag
     6841 cgtggcgacg tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct
     6901 taacttctgt gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg
     6961 gtgttatctt ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt
     7021 tt
//
LOCUS       DAYYNQ010000120         6759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000120 DAYYNQ010000000
VERSION     DAYYNQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.122"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHBJ1_002620"
     CDS             complement(<1..182)
                     /locus_tag="ACHBJ1_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM9431503.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHBJ1_002621"
     CDS             517..951
                     /locus_tag="ACHBJ1_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM9431504.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHBJ1_002622"
     CDS             974..1294
                     /locus_tag="ACHBJ1_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431505.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHBJ1_002623"
     CDS             1435..1626
                     /locus_tag="ACHBJ1_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431506.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHBJ1_002624"
     CDS             complement(1697..1954)
                     /locus_tag="ACHBJ1_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM9431507.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHBJ1_002625"
     CDS             2112..2375
                     /locus_tag="ACHBJ1_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431508.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHBJ1_002626"
     CDS             2399..2587
                     /locus_tag="ACHBJ1_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431509.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHBJ1_002627"
     CDS             complement(2670..2855)
                     /locus_tag="ACHBJ1_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431510.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHBJ1_002628"
     CDS             3070..3441
                     /locus_tag="ACHBJ1_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9431511.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHBJ1_002629"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHBJ1_002629"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHBJ1_002630"
     CDS             complement(3709..4137)
                     /locus_tag="ACHBJ1_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431512.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHBJ1_002631"
     CDS             complement(4197..4955)
                     /locus_tag="ACHBJ1_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM9431513.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHBJ1_002632"
     CDS             5250..5447
                     /locus_tag="ACHBJ1_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431514.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHBJ1_002633"
     CDS             5553..6035
                     /locus_tag="ACHBJ1_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431515.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHBJ1_002634"
     CDS             complement(6093..6689)
                     /locus_tag="ACHBJ1_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9431516.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2280 a         1045 c         1334 g         2100 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcacctta
//
LOCUS       DAYYNQ010000121         6661 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000121 DAYYNQ010000000
VERSION     DAYYNQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.123"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1273..1938)
                     /locus_tag="ACHBJ1_002635"
     CDS             complement(1273..1938)
                     /locus_tag="ACHBJ1_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431517.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="ACHBJ1_002636"
                     /pseudo
     CDS             2118..4100
                     /locus_tag="ACHBJ1_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4104..4742
                     /locus_tag="ACHBJ1_002637"
     CDS             4104..4742
                     /locus_tag="ACHBJ1_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431518.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="ACHBJ1_002638"
     CDS             4770..5198
                     /locus_tag="ACHBJ1_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431519.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="ACHBJ1_002639"
     CDS             5655..6566
                     /locus_tag="ACHBJ1_002639"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM9431520.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2294 a         1009 c         1222 g         2136 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agatagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 c
//
LOCUS       DAYYNQ010000122         6527 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000122 DAYYNQ010000000
VERSION     DAYYNQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.124"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1278
                     /locus_tag="ACHBJ1_002640"
     CDS             <1..1278
                     /locus_tag="ACHBJ1_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM9431521.1"
                     /translation="LGATSALTIVILILSLFFVIRSSTKKEPIIPEKKITEKAKEPTD
                     DKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQLIDWSDPTQ
                     ISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLVNLNDLIQK
                     DEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEIKNVYKEKD
                     KEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQYGDNEKTKK
                     EIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAPILGKTNIL
                     IYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFYRNARINID
                     GIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1347..1946)
                     /locus_tag="ACHBJ1_002641"
     CDS             complement(1347..1946)
                     /locus_tag="ACHBJ1_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9431522.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            2200..3081
                     /locus_tag="ACHBJ1_002642"
     CDS             2200..3081
                     /locus_tag="ACHBJ1_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9431523.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            3094..4704
                     /locus_tag="ACHBJ1_002643"
     CDS             3094..4704
                     /locus_tag="ACHBJ1_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431524.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(4781..5440)
                     /locus_tag="ACHBJ1_002644"
     CDS             complement(4781..5440)
                     /locus_tag="ACHBJ1_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9431525.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(5723..6388)
                     /locus_tag="ACHBJ1_002645"
     CDS             complement(5723..6388)
                     /locus_tag="ACHBJ1_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM9431526.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1876 a         1263 c         1227 g         2161 t
ORIGIN      
        1 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
       61 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
      121 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
      181 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
      241 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
      301 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
      361 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
      421 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
      481 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
      541 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
      601 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
      661 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
      721 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
      781 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
      841 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
      901 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
      961 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     1021 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     1081 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     1141 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     1201 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     1261 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     1321 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     1381 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     1441 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     1501 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     1561 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     1621 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     1681 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     1741 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     1801 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     1861 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     1921 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     1981 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     2041 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     2101 aaaataataa aaaaagtttt ataaaaaggt ataaaacctt tttattaaac tatttaatct
     2161 tgtttagtat atatttttag aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc
     2221 acacatttga aaaagagctt tggtcgtttc gaagctttaa aagatgtctc tttcaccgta
     2281 cattcaggtg aagtcgttgg atttatcgga ccaaatggtt ctggaaaatc cacgacgatt
     2341 cggacacttt tagggattat aaaaaaagac ggcggaacgg caacgatttt tgggcaagat
     2401 gtatgggaag atagtttatc tatccatgaa cgtctttctt atgttccagg cgatatctct
     2461 ttgtggggaa atttgactgg aggagcgatc atcgatctct tcatgaagct tcatggaaaa
     2521 gggagtgctc aaaaaaaaga tgaactgatt caacgtttct ctcttgatcc taagaaaaaa
     2581 gcaaaaaact attcaaaagg aaatcgccaa aaagtgggct tgattgctgc tttggctgtt
     2641 gattcggatc tctatttatt tgatgagcct acttctggtt tagatccttt gatggaaacg
     2701 attttccaag aagaagttga aaaattgaaa gaacagggca aatccatcct actttcttct
     2761 catattctca gtgaagtcga gcgactagcg gataaagtcg tgatcatacg cgaaggaaca
     2821 ctcgatgaag aaggaacact cgatgaattg cgtcatttga ctcgttcgac catcactata
     2881 gaaacagaag cagagaatag tcacttagca caattgaacg gtgtccatga ctttgtgcaa
     2941 aaaggtcacc tcgctcaatt tgctgctgat aacgaagctt tcgatgtgat tttagctgaa
     3001 gcgtccaaat taggtgtgaa aaaatttgaa tctattccac cgactcttga ggatttattc
     3061 atgagacatt atgaaggatg aggaggaagc gaaatgactg ataaattttc acgctggtcc
     3121 accctttttt ctcaaaacct tagccgtgac tggaaaaaga tcctgatatg gctagtcggt
     3181 gtcactgctt tttctggtgg ttttgtccct gcgtttgaag aattgacttc cggacaagga
     3241 ttagctggat tatacgaaac cttgcaaaac ccggcaatga ttgcaatggt cggaccaact
     3301 ccagcttcga atgctgctga gtatacttta ggagcgatgt atgcaaatga aatgttgctt
     3361 ttttgcagtt tgtttgctgc tgtgatctct gttttacacg tggtgagcca aacgagaaaa
     3421 aaagaagaac ttggtctgat tgaacttatt cgttcctttc gagtcggacg tcaagcaaac
     3481 tctttagctg tcatcgctga aagtattctg atcaatttgc ttttagcaat atcgatcagc
     3541 tgtacgatga tttttttcca agcggattcg atcactgcag aaggtgcttg gctttttggg
     3601 ctatctgtag gtagtatggg gattttgggg agtgccgttg cactgtttgc tgcacaactg
     3661 atgccaagtt cagccagttc gaatggtctg tctttagcgt ttttaggtgg actttacctt
     3721 ctacgagcaa taacagatgt agctgattcc tccctttcga tgtggaatcc tttaggctgg
     3781 tcttatttga cctatccatt tacaactaac aactggattc ctttgcttta tttgtttttg
     3841 ttcagtctaa tcttgataat cagttcattt gttttagagg aacatcgaga tttgaacacc
     3901 ggctatatcc ccgaacgtga aggaaaaccg tatgctaaac gttctctttt atccttgcgt
     3961 ggtttattat tctatctaaa taaagggatg acaatcagtt ggcttatcac ttttggtttg
     4021 atgggggcgg catacggctc tatctacggg aatatgcaac agtttttaga aagtaacgac
     4081 ttaatgaaac agatgtttat ccaatctggc gtctcaattg aaaaaaactt tacaagcacg
     4141 atcatggtag tcttgatttg tctagctgct attttaccaa ttgctagcat caatcgttta
     4201 tttacagaag aaacccacgg acgtatgaat cagctgttcg caacaaaaat ttctaggaac
     4261 caactttatt ggaccaatat gttactagct attctatttg ctatattggc aatcctgatt
     4321 agttctggtg cactagggct ttctgctttg gcttcgatga aggatgcgaa agacatggat
     4381 ctattgatgt ttcttaaagc cgggttcaat ttcctgcctg ccgttttagt gtttatcgga
     4441 attagtggct ttctattcgg atggcttcct cgaatcggta aacttgctta tatctactta
     4501 gcttactcct ttgcactcaa ttactttggc ggaattttgg atttaccaga atggttttcc
     4561 aaaacagctg tccctagctg ggttccacgg atgccaaacg attcttttga tctttccgtg
     4621 ttccttacgt tatcatgtat cagccttgca ctgttcgtcc ttggttctat tggttatagg
     4681 cgaagagact tactagaagg ataataaaaa agcatgtaca atttcatgct agcctccaaa
     4741 tctgggtcaa aaatccaaca tttggaggtc atttttttat ttacaagtct tctaactgct
     4801 ctttatcaat gatccagact tctcgattct ttctttttac ataaccattc tcctctaata
     4861 atttaaattt tctagataaa gtttcggggg tcgtaccgat aaatgcagca agttctttca
     4921 ttttcattgg caggaaaaag tgatccttcc ctgtcgcttt agctaagtca agcagataga
     4981 gagcgactcg ttcttctact ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg
     5041 ttaatttctc aacgttgatt gctaaaagtt tcagactaag ggcgggataa tgcagtaaga
     5101 gttccgagaa atctttttgt ctcaatatgc aaatttccgt tttttctagt gcttcgccaa
     5161 ataaagactc atttggaacg cctagcaact ggctctctcc ctcatatccg cccggctcta
     5221 ttactcgtaa aagctgttcc tttccgctag cagataattg atacactttc atactgccgg
     5281 aggccacgat cactaattga ggttctccat tcggcgcaag aatctgttct cctttttcca
     5341 catagtgatg tttgacaagt gcgtgtactt tttcttgatc ttgctgtact aagtgattga
     5401 acaaaggaac aaatgaaaca cataaatgat catgactcat aatctctcct ccttctaaac
     5461 agctatttct aataaagaaa cataacttcc cttattccaa ctgataattt tcttttatca
     5521 tagcataaac gtcaagcgaa ccagatacca gcaaatagag tggatttctt tttgtccctt
     5581 gctttatttt ccttcttttg agaccttaag taattgattc ctatcaattt tttaaatatt
     5641 tactttttga ataatcaaaa acaaaccttc actcgcaagc cgagagtaag gtttgttttt
     5701 cgcattgctt atttcgtgtt gtctattgaa atttttccgc acctagcttt tgatatagtg
     5761 gtgctagttc ttgtaaggtt tcagcggtta tttgctcaac gtgttcgatt tcttgcggat
     5821 agaagaatag tttacccatt tgaaaggttc ggtctctcat cgcttttttc aactcttctt
     5881 gtttctttgt atcggacaaa tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg
     5941 gttgcggcca tatgtaataa tccttgatat cgactgtttt gctccactct ttcagtatcg
     6001 caagcaactg gttatacgct gcaatgctac catatcgttc ttcgctttta taatgttggt
     6061 acatcaaata gatttggtat tgtttttgat tcaacaaaac tgctagacag gcattctcgt
     6121 cttgacgaga ttcgcttcgg tacgcgctcc aaaaatgact gcgtaaattc cagccattcg
     6181 tccagctttc gattttaggt ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct
     6241 ggagctgaat ctctttccat ttttgccagt gttctttata ttctttcttg atttgttcaa
     6301 tcttatctgt tggatggctc tctttgagct gtttgaatgc gtagattggt cgatcaaaca
     6361 actgataatc tgattttcca agttccattg tttcgcctct ttccgttatc ttttgtattc
     6421 tacctgaatt ttgcgatatg gacaaataga ggttgtgaaa aagctgttct gcatcaagtc
     6481 ataagaacag ccaaacaaaa aactgcgatg agcatcaccg cagtttt
//
LOCUS       DAYYNQ010000123         6347 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000123 DAYYNQ010000000
VERSION     DAYYNQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.125"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..1135
                     /locus_tag="ACHBJ1_002646"
     CDS             290..1135
                     /locus_tag="ACHBJ1_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431527.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="ACHBJ1_002647"
     CDS             1416..2636
                     /locus_tag="ACHBJ1_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM9431528.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="ACHBJ1_002648"
     CDS             2633..5353
                     /locus_tag="ACHBJ1_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431529.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="ACHBJ1_002649"
     CDS             5337..6281
                     /locus_tag="ACHBJ1_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM9431530.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          983 c         1224 g         1777 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttc
//
LOCUS       DAYYNQ010000124         6303 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000124 DAYYNQ010000000
VERSION     DAYYNQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.126"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACHBJ1_002650"
     CDS             334..714
                     /locus_tag="ACHBJ1_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9431531.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="ACHBJ1_002651"
     CDS             696..1610
                     /locus_tag="ACHBJ1_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9431532.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1570..1848
                     /locus_tag="ACHBJ1_002652"
     CDS             1570..1848
                     /locus_tag="ACHBJ1_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431533.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            1855..2544
                     /locus_tag="ACHBJ1_002653"
     CDS             1855..2544
                     /locus_tag="ACHBJ1_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431534.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="ACHBJ1_002654"
     CDS             complement(2665..2751)
                     /locus_tag="ACHBJ1_002654"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9431535.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="ACHBJ1_002655"
     CDS             3427..4377
                     /locus_tag="ACHBJ1_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9431536.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="ACHBJ1_002656"
     CDS             4398..4931
                     /locus_tag="ACHBJ1_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9431537.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="ACHBJ1_002657"
     CDS             4947..5117
                     /locus_tag="ACHBJ1_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431538.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="ACHBJ1_002658"
     CDS             5567..6100
                     /locus_tag="ACHBJ1_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431539.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            6112..>6303
                     /locus_tag="ACHBJ1_002659"
     CDS             6112..>6303
                     /locus_tag="ACHBJ1_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431540.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DAYYNQ010000125         6302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000125 DAYYNQ010000000
VERSION     DAYYNQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.127"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBJ1_002660"
     CDS             complement(41..229)
                     /gene="rpmB"
                     /locus_tag="ACHBJ1_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM9431541.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="ACHBJ1_002661"
     CDS             499..861
                     /locus_tag="ACHBJ1_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9431542.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="ACHBJ1_002662"
     CDS             913..2595
                     /locus_tag="ACHBJ1_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM9431543.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="ACHBJ1_002663"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="ACHBJ1_002663"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM9431544.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBJ1_002664"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="ACHBJ1_002664"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM9431545.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBJ1_002665"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="ACHBJ1_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM9431546.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2094 a         1095 c         1437 g         1676 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaaggatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 tt
//
LOCUS       DAYYNQ010000126         6217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000126 DAYYNQ010000000
VERSION     DAYYNQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.128"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHBJ1_002666"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHBJ1_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHBJ1_002667"
     CDS             complement(1878..3080)
                     /locus_tag="ACHBJ1_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM9431547.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHBJ1_002668"
     CDS             complement(3140..3343)
                     /locus_tag="ACHBJ1_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM9431548.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4549)
                     /locus_tag="ACHBJ1_002669"
     CDS             complement(3485..4549)
                     /locus_tag="ACHBJ1_002669"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM9431549.1"
                     /translation="MDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERFE
                     ELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQF
                     FRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINNE
                     SNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAKI
                     ISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQSF
                     EKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYYQ
                     EEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHBJ1_002670"
     CDS             complement(4610..4717)
                     /locus_tag="ACHBJ1_002670"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431550.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHBJ1_002671"
     CDS             4880..5692
                     /locus_tag="ACHBJ1_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431551.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1841 a         1280 c          991 g         2105 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatt
//
LOCUS       DAYYNQ010000127         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000127 DAYYNQ010000000
VERSION     DAYYNQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.129"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHBJ1_002672"
     CDS             725..811
                     /locus_tag="ACHBJ1_002672"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9431552.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHBJ1_002673"
     CDS             complement(932..1621)
                     /locus_tag="ACHBJ1_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431553.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACHBJ1_002674"
     CDS             complement(1628..1906)
                     /locus_tag="ACHBJ1_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431554.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACHBJ1_002675"
     CDS             complement(1866..2780)
                     /locus_tag="ACHBJ1_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM9431555.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHBJ1_002676"
     CDS             complement(2762..3142)
                     /locus_tag="ACHBJ1_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM9431556.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHBJ1_002677"
     CDS             complement(3592..3879)
                     /locus_tag="ACHBJ1_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9431557.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHBJ1_002678"
     CDS             complement(3899..4123)
                     /locus_tag="ACHBJ1_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM9431558.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="ACHBJ1_002679"
     CDS             complement(4578..4793)
                     /locus_tag="ACHBJ1_002679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431559.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHBJ1_002680"
     CDS             complement(4763..5296)
                     /locus_tag="ACHBJ1_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9431560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHBJ1_002681"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHBJ1_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM9431561.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYNQ010000128         5939 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000128 DAYYNQ010000000
VERSION     DAYYNQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.131"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1193)
                     /locus_tag="ACHBJ1_002682"
     CDS             complement(<1..1193)
                     /locus_tag="ACHBJ1_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9431562.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHT"
     gene            1472..2278
                     /locus_tag="ACHBJ1_002683"
     CDS             1472..2278
                     /locus_tag="ACHBJ1_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9431563.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2296..3003
                     /locus_tag="ACHBJ1_002684"
     CDS             2296..3003
                     /locus_tag="ACHBJ1_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9431564.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3000..3971
                     /locus_tag="ACHBJ1_002685"
     CDS             3000..3971
                     /locus_tag="ACHBJ1_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9431565.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3964..4992
                     /gene="vanB"
                     /locus_tag="ACHBJ1_002686"
     CDS             3964..4992
                     /gene="vanB"
                     /locus_tag="ACHBJ1_002686"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9431566.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            4998..5606
                     /locus_tag="ACHBJ1_002687"
     CDS             4998..5606
                     /locus_tag="ACHBJ1_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9431567.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5672..5845)
                     /locus_tag="ACHBJ1_002688"
     CDS             complement(5672..5845)
                     /locus_tag="ACHBJ1_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431568.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1679 a         1218 c         1499 g         1543 t
ORIGIN      
        1 tgggtatgca gattttctat tttggcattt gaataaggca attcgagggc taaacgaatg
       61 ccggattcat agtttataaa agcttttttt atagaatgca gttcttttgg aacggaattg
      121 ggcatcgttc ttacaagttt aaagaaagtg tccgcctctt tgtcgtgaaa cgcttgaaga
      181 atttcttgat agtaaaaata ggcgattcgt aattcgtcag agatcgaaag cagacgatcc
      241 accacttcac tttcggtgag atacgcccaa ccaaaattgc gccatttggt ccatttgtca
      301 taaacaagct tgtcctgtcg tttgatcaga agccgagaca acgcctttaa ttgtcgataa
      361 tatttggctt gtgttggatc gtttcgatca aagcttttca tgattcgtac gcgaatatcc
      421 tcaaatttac gaatgagatg tttgacaatg tgaaaacgat ccacaacgag ttttgccttt
      481 ggaaaacatt cctttatcag agaggcataa gaaacattca tgtccgtcac gatgaccttt
      541 accttcaatc gtgcgcgtcg ttcaaattgc ataaaataag aaacgagagc ctttttcttc
      601 cgtgaaggaa gaatatccag taattttcca gtatcgccat ccatacaaga aaaggagtag
      661 ggaccgactt gatttttcag tgaacgaaat tcatccatta ataaacaacg cggtaaccaa
      721 tttcttttta cttttgtcat ggggatcaga gaagcgagtg tccgagaaac ggtcgaccaa
      781 gaaacgccgc aatgcttagc aattgtcgtc attgcttgtt tttcagttag ctcttctaaa
      841 attttatagc gcacttgttt agcgataaag cagtttctat cgacaatgta ggtattcgcc
      901 gtaaaatagg tgtggcagcc acgacagtgg tagcgttgtt tcgctaaagc aagataagtc
      961 agttggttcg cacatttatt aagttgaatg agcgattttt tctttccgtt cttaacgatc
     1021 atgccttcat gattcatctg tttacacaaa ggacagcgac gaatttcttt acttaacgtc
     1081 gcagaaacaa caagactctt gatattctta atgctcttcg attccacatt tgtgatgaaa
     1141 atgtttttat ctttgattcc aagtagtagt ttgatatgat gaagatcagg catacagaac
     1201 gctcctttga ttgtgttgtg gtgattcaat tctaacggag cttctgtatg ttttgttttt
     1261 tcatgctcaa aagggaccga tagaatgatt ttctatcagt cctagaaatt atagagccga
     1321 ttttgaccga caggtataac cctgccggtc tttttttgct gctacaggaa aactacagac
     1381 tggctacagg gaaagtacag atgcgcttgc cataataaca atcgtaccag ccacaaatcg
     1441 tagttttatt gcaaaggagg cattcgatca aatggaaaaa agcaactatc attccaatgc
     1501 ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt
     1561 cattatttcg ttcacagtct gcacgctgtt tttggggtgg agactggctt ccgtattgga
     1621 ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg acgtagtgga
     1681 gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac aggaatggag
     1741 cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg agcttgagca
     1801 actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag atttgtttga
     1861 tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa caacagaaaa
     1921 acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct acacctctgc
     1981 acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg agcatcagct
     2041 tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg aggtttatag
     2101 atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac cagacaagac
     2161 agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc
     2221 cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga
     2281 aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca
     2341 atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc
     2401 tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca
     2461 ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta
     2521 aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc
     2581 tggttgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag
     2641 tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc agagggctgg
     2701 attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt gaccttagat
     2761 gatgagaaaa tcatcggtca ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt
     2821 acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt
     2881 aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca
     2941 aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga ggagaaccaa
     3001 tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca aatgctttcc
     3061 gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg atatcggcag
     3121 acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aagccataag tccgaggttt
     3181 ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct acccgcagca
     3241 tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg gttggcacgg
     3301 ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg atggccatac
     3361 ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc
     3421 gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat atcgggcaag
     3481 cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat aacagccgaa
     3541 aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat attgttacgc
     3601 tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa attggagaga
     3661 tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat accggggcgc
     3721 tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg ttggagggcg
     3781 aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc tttttgtcgc
     3841 agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac actgagcgtg
     3901 tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa aggagtttac
     3961 agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga acatgatgtg
     4021 tcggtaaaag ccgcaataga aattgctgcg aacattgata cggaaaaatt cgatccgcac
     4081 tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg tacggaatgg
     4141 gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg gctgcttgtc
     4201 atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt tttgcatggc
     4261 aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat cccctatgtg
     4321 ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta cattcttaca
     4381 aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg ataaaggtga caagccggag
     4441 gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc gtcctttggc
     4501 gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc aggacaatat
     4561 gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg
     4621 gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc
     4681 ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat gattacagtt
     4741 cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat
     4801 cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga ggatggcggc
     4861 atcgttctaa atgaggtcaa taccctgccc ggttttacat cgtacagccg ctacccacgt
     4921 atgatggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat tacattggcg
     4981 ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg
     5041 tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg gtggatgggt
     5101 atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa
     5161 tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg ccaagaactg
     5221 cggtggactg cttcctgcgt tgggcagcgc aaccggagga caatctcaca aaagaaaaat
     5281 tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc tcacaatcca
     5341 gccatagccg tggaagcgca attgatctta cgctctacca cctggataca ggggaacttg
     5401 tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcataca gcaaaaggga
     5461 taggggatgc agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa agcagcggat
     5521 ttcagtctta tcgctttgaa tggtggcact ataagttgat tgatgagcca taccccgata
     5581 cctattttaa ttttgccgtt tcataatgaa agtattggtt ttgtatcaga ttttggattt
     5641 gataaaatct atgctggtca tggacaatct tttactggaa agtggttaaa gcatcttttg
     5701 taatacattg caaattatag tattcaatct gcccagcggc agaaaagaaa aaaaccgctg
     5761 ttgggcagac ctttcccacg cccattttgt attttgctgg cggcggtttt gagaaatcaa
     5821 ggccgccgtt cttttctggc agcaaaaggt gtggcggccc ataggaggat gccgctatgc
     5881 tgttttgcta ttgccatgct ctaatccact tccaccactc aaaaaaagcc ttgcccctc
//
LOCUS       DAYYNQ010000129         5814 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000129 DAYYNQ010000000
VERSION     DAYYNQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.132"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..2430
                     /locus_tag="ACHBJ1_002689"
     CDS             370..2430
                     /locus_tag="ACHBJ1_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM9431569.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2513..3082
                     /locus_tag="ACHBJ1_002690"
     CDS             2513..3082
                     /locus_tag="ACHBJ1_002690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM9431570.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3257..4063)
                     /locus_tag="ACHBJ1_002691"
     CDS             complement(3257..4063)
                     /locus_tag="ACHBJ1_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431571.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4201..5715
                     /locus_tag="ACHBJ1_002692"
     CDS             4201..5715
                     /locus_tag="ACHBJ1_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM9431572.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2081 a          995 c         1162 g         1576 t
ORIGIN      
        1 tggaaaaatc caccgactcc agatgactgg gaatattttg gaaataccat catcagagaa
       61 aaaaatagac atttaactga aaaaatcagc tagtatttat ggatggcagc tttgtctcac
      121 atatttatta gctgatttat gtttttttca aagaaaaggt aactagatat aactagatat
      181 aactagatat attacttaac aaaattaaaa agtacataag ttattattcc gattttcttg
      241 aaacaataaa aaatcaaaaa aaatactctc ctaatagtcc atcttctttt gattttattt
      301 atataatgaa atcatcttat gaaaattact acttcatgtt tagacaagag tagggaggac
      361 taagaataaa tgtgttcaaa aagacgttca aaacaactaa ataaacacgc ttgtcgttta
      421 gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg
      481 atcagtttat ctgtaggtgt ctatatgcat gtacccgttc aggcaacgac acaaaccatc
      541 aaggtcgtac gtacctctgt ttctgagacc actgattccc ctatcactat tactcatact
      601 ttcactgatg caaatggaaa acagctactt cctgcattga cttcctataa taaagtcagt
      661 tatcctccaa cgataaacca ctactggtta attcctaaca aaacattatg gacatcggaa
      721 accggaagca gtgtgccgct ttctatcttt tccgatatga ctgatcctac aaaaggtgat
      781 cagttaaaac agatcactga tattttgaat catgctttga cagatataaa ggacggtgga
      841 agcgcacaag tttcttatgt atatgaaaaa gattattcac agtttgaaag aaagaaaatc
      901 gatgttctgg cagggacaaa agtttcgata tctgatctgg tagaaaggct gaagaacgca
      961 gatggaacac aaggagatat gtccaaagta atttcaaaca atgggaatat cgtggatacg
     1021 actaatacag gaacatctac tatttccttg tcctattttg atccaattgc tttaaaaaca
     1081 atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat
     1141 cttcacttaa gaacaggaga gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa
     1201 agcgatggaa cttcaggaaa taaagaagaa atcaaaatga acgggacagt agatactaca
     1261 actccgggag aatatcccgt tgtattaact tatatagact cgaaaacatc aaatgtggca
     1321 gaaacaacag ctgtagttta tgtagaagca tctgctgctt catcgactgt catcactcat
     1381 tttatagata gtcaaacaaa taaagaactt caagcagaaa cacatatgag tagtgtaaat
     1441 cgtattcctg atttgcgcct acctgctgaa ttttctgatc gagtgccaaa tatagattta
     1501 tcgacttatg aacaaaatgg gaaagtcact tctatttctg attggatgca agcaaacgga
     1561 ttagacagaa aaagtcactg gtcggagatc cttgaagtac ttcactcaga actgatgaat
     1621 aataggaagc aatcaggagg ggaaattacc tttacttatg tttatatacc agacaatcgt
     1681 tcattagaag gtcatgatct tattgttcat ccaaacgaaa aaataaacaa agaagagttg
     1741 atccaatcag caacaaattc cttaggtaaa cccgtaccgt taaaggatgt atcggttaca
     1801 atcaatgaag taccattgga ggatggttat ttgaccaaac aaacaggtga ttttacgatt
     1861 atgtatagtt atcatgaccc gtattcactc caagaactcc atgctcagtc actattacat
     1921 gttttaccat taaacaaaca gatagatcaa aaagaacaaa aaagacaaga agaaagcttg
     1981 tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa cagggaaagt aaaacaaaca
     2041 agaagcctag agttagcaag aatatctaga aaaatctcag aatctaataa aacgataagg
     2101 caagaagctc atttggcccc acaagtttct ggattggaaa atgaaatttt agagcctaaa
     2161 ataaaacaac aatcagaaaa agaatttgtt gctaaacagt cggaagatca gaaggaatca
     2221 gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat
     2281 ctgcagccgc aagtaccact tcctagtccg ggaacacccg cacctggtgg aggtattcct
     2341 tcttctgctt taggagattt catgcgaggt atggcaggaa catgggtata cgctaatcga
     2401 aatgaatttg acgcagatga ctggttctga tttatatctc tatctgttat agtcaaataa
     2461 gtagctgagt catatgttat ttggcataat ggagacgagg agggatagaa aaatgttaga
     2521 taaaacgtta ccttacgcag aaatctggat gacaagacca cttcaacaaa atgttccaat
     2581 cacgccttta gcggaaggtt ttgaatttcg attttatcaa aaagaagatg aatctgcttg
     2641 ggcggagatt gagacttccg ttggggaatt tcaaacgaca gatgaagcac ttgagtattt
     2701 tcaacaaaac tttgctcctt atcctgaaga acttgaaaga aggatgtttt ttgtagagac
     2761 aaaaaaagga gaaaaagtag caacctgtac cgcttggcga aaaaaatcaa gagagcaagt
     2821 gtatccggtt tttcattggt tagcagtgaa atcagattac caaggaaaag ggctcgcaaa
     2881 agcattgact gccagagtct tgcaagaatt tcctaccctc catacggacg gaccaatcta
     2941 tcttcacaca cagacatgga gccatcaggc gattgctctt tataaaaaaa tgggttttac
     3001 ttttattgct gaaaatctag acggcacacc taatccagat tatgaaaaag ttatgcaggt
     3061 tttaagtaaa ttagataaat aacgaaatgg tgaaataagt cctcagtatt gggataaaaa
     3121 acgaaatagt cattcctcat atcttacttt tttgagttaa tactatcgga taactaatgg
     3181 catgccatac attcaaaaaa gaaggggcag tgacaaaagt attgtcactg ccccttctct
     3241 tgttttttag catgttttat cgtttgattt ttttaggaat aaaattcttg atgaacgtaa
     3301 atttttggta atcgtttcga ctatagaggt aaccaaatac actgaaaaca actgctccaa
     3361 taaaattaac aaataaatcc ttcatagtat ccatgattcc cacgtctaag taaccatttt
     3421 ggatgattgt tttatctgtt ttaccatctg cattttgact ttcaatgatt gtccgagtga
     3481 tatctggaac atgatagaca gtgtttccgt ctttacttaa ctccacacta ttgatttgct
     3541 ggatgatcgt atctttttgc atatcgaatt gcagtaattt atctgctgaa aattccacaa
     3601 actcccacat aacaccaact gtcatcgaaa aacagaagga aacaactgct agtaataaag
     3661 gagtcaagtt catggttttt gtatttttat tgagcaaata aacaagggaa aagccgacac
     3721 ccccagctaa aaatccgctg aatgcatgta gaaaagtatc ccacattgaa aaatagccat
     3781 aaaaatcact taactctcct aaaactgttg aactaaaaat aaaagataac aaaatgattt
     3841 ctagcatatt tggaatctgt atctttaata tcttttccag aaaataaggg atttcaaata
     3901 aaattaatgt cagtaataaa agcattgtat ttccccagtg ttggttccag acctgatcca
     3961 ctagcataat aacgacgatg atacgtaata aaatatagat tccgtttgtg attctacctg
     4021 cagtttttgt actgttcgta aaaaaggttt tcaaatgatt cataaaataa ctccttcatg
     4081 tatatcctta attatactaa gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa
     4141 cagagttgat attgtgaaaa aaatcactta atatagtata aggagaaaag ggggatacaa
     4201 atgaaaaaat tagttgctgg tttattaaca gccgttgcac ttcttgctat gagcgcatgc
     4261 ggcgcagatc agaaaaatac agcgcagaca agttctagta cgaaggaatc tgccgaagta
     4321 tcatcaggcg cttctgaaca agtatataca gatccaaaag aaatgaaaga atcttatgat
     4381 gtcgtgatta ttggagctgg tggtgcaggg atggcagcag ctatcgaagc aaaagatgct
     4441 gggttgaatc cagtaatttt ggaaaagatg cctgttgccg gaggaaacac atctaaatca
     4501 tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag aagaaggaat caaagactcg
     4561 aatgatgcct tctatgaaga aacgctaaaa ggtggtggag gaacgaatga taaagaaatg
     4621 cttcgatact ttgtcgatca ctctgctgat gcggtcgatt ggctagatca aaacggtatc
     4681 aaactggaca acttaacgat cactggaggg atgagtgaaa agaggacaca tcgtccgtct
     4741 gatggctcgg ctattggtgg atacttagtg gaggggttat tgaataacgt ccatgagcgg
     4801 gaaatcccta tttttgtcaa cgcagatgtg acagacatta agaaaaatgg ggaaacggtc
     4861 aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc
     4921 gtagttacca caggcggttt tggtgccagc aaagaattta ttgaaaaata tcgtcccgat
     4981 ttagctgatt atattacgac aaatcaagaa ggatcaactg gagatggtat caccatgatt
     5041 gaaaaattag gtggacaaac cgttgatatg gataaaatac aaattcatcc aactgtgcag
     5101 caggatgagg gggtcttgat tggtgaggtt gttcgcggag aaggagcaat cttggtcgat
     5161 caagagggga aaagatttgt caatgaaatg gatacaaggg ataaagtatc tgcagcaatt
     5221 acagcattgc ctgaaaaatc agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc
     5281 acagctattg aattttatga ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa
     5341 ttagcagaaa aaatcgattt accaaaagaa acactagcaa aaacagttga tggatggaat
     5401 acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg ccacagccat ggaacatgac
     5461 ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag gaatccatta cacgatgggc
     5521 ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag aaaaccagcc aattaggggg
     5581 ttatatgctg ctggtgaagt cacaggtggc ttacatggaa acaaccggat cggtggaaat
     5641 tcagttgcgg agatcattat ttttgggcga caagcgggca tacaagcagc tcgatttgct
     5701 tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc
     5761 taataccgag ggtcagctga ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DAYYNQ010000130         5727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000130 DAYYNQ010000000
VERSION     DAYYNQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.133"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHBJ1_002693"
     CDS             196..921
                     /locus_tag="ACHBJ1_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431573.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHBJ1_002694"
     CDS             911..1420
                     /locus_tag="ACHBJ1_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431574.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHBJ1_002695"
     CDS             complement(1490..2938)
                     /locus_tag="ACHBJ1_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9431575.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHBJ1_002696"
     CDS             complement(2938..3654)
                     /locus_tag="ACHBJ1_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM9431576.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHBJ1_002697"
     CDS             complement(3635..3985)
                     /locus_tag="ACHBJ1_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM9431577.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHBJ1_002698"
     CDS             4129..4902
                     /locus_tag="ACHBJ1_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM9431578.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHBJ1_002699"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHBJ1_002699"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAYYNQ010000131         5722 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000131 DAYYNQ010000000
VERSION     DAYYNQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.134"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(176..1159)
                     /locus_tag="ACHBJ1_002700"
     CDS             complement(176..1159)
                     /locus_tag="ACHBJ1_002700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9431579.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            1537..2928
                     /locus_tag="ACHBJ1_002701"
     CDS             1537..2928
                     /locus_tag="ACHBJ1_002701"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM9431580.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            2944..3984
                     /locus_tag="ACHBJ1_002702"
     CDS             2944..3984
                     /locus_tag="ACHBJ1_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9431581.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            4003..5088
                     /locus_tag="ACHBJ1_002703"
     CDS             4003..5088
                     /locus_tag="ACHBJ1_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM9431582.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            5121..>5722
                     /locus_tag="ACHBJ1_002704"
     CDS             5121..>5722
                     /locus_tag="ACHBJ1_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFM9431583.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTEL"
BASE COUNT         1990 a          862 c          998 g         1872 t
ORIGIN      
        1 agaagaagtt gcttctgctc ccaccgttta ttcgttttta aagaatgaga caaaagtgtt
       61 tcttactttt gtcccacgct cttattcatg ctcagagcgc caatagatac aaaatcatag
      121 ccaaagtttc acgaacccta gctgtcaaag aatcgatatt ttattttaat ataggctatt
      181 ttaaaataaa tggtttaagg tattcctcta caaaagccac tctatcaatt atcatgggtt
      241 taaaataaga agtgatggag ataaccagat tgttcttagc gtcaatataa agaacatttc
      301 cactattacc aattgctgaa tatatttcgt tctcagaatc taatacccac cataaatagc
      361 catatctcat attttgtaag tcttctaaag cactggttct aggtttgaac atctcgttta
      421 tccacatggg tggtacgatt tgttttcctt tatacatccc tttatttagg cataatacac
      481 caatttttgc cagatcactt gccgataaac acaaaccata tcctgccgtc ccgatattta
      541 gtggatcaca aaaccagaca ttggttttag gagacttatc cattgtaaac tgcttatgtt
      601 cttcagctgt ttcagcataa taattgacat ggtttggtat ccctatagga tcaaatagat
      661 acttattggc aaaatcaaca gttgttagct tacttgtctg atacaacaag ccagttaata
      721 tgtgtaaaca gacagtttga tagttgaatt cgccggttat accttttctt cctcccaata
      781 aatctagtga tgtttttgtc caatcttcac ttgaacagac ttttgtccat ggatcgcctt
      841 tacatttgta gggtgctttc attgttagca agtgcttgat tgtaatagcg taaatagttt
      901 tctccccgcg tttcacttta tactctggaa aataatctaa tactttatca tcgatacttt
      961 tgattaatcc ttgctctaaa gcaatgccaa tcagcaaaga tacaatactt ttcgttgctg
     1021 acatgatatg acaagtatcg tcttttttat aaccattcca ttcatctgta taaacttcta
     1081 ccccatcctt atatgcgaca atttgacata tatttggttg tcgaaaacca ataaaatcat
     1141 gcaactcttg tttattcata agatattacc tcactttctt ctataagcat aatgtcatta
     1201 aaaatcgatt tcaatttttt cgattcttca cgtaatacag gtattcttat ggactagaca
     1261 gcgctttggg aagatggatt ctctgaaaaa tataaaggtt cactttatga gtggataaaa
     1321 aaagcagcaa atacttcgac ttctttaatt taccaacatt agtgaaaatc gattcatctg
     1381 gagatttgta atttagagtc cttgtcttga ttagaggtgt ataaagcttt ttattagaca
     1441 tttttaaatt ccattacaat ttgcttataa actaggtttt taaaggtgac tatggtaaga
     1501 taatagagat attattttgt gattaggagt gtatatatga ataaaaatgg agaatggagt
     1561 gctgcacgac ggatattcat tatattaatg gatgttattg tatacaatgc agcaatatat
     1621 agtagtttct tactgaagtt tcgtggagag atcccattac gaaatttaga aactttccaa
     1681 cactcagcaa ttattatttc agtgattttt attgcgttga acattttgtt gggtgcatat
     1741 gttttttata atcgaatgat cagtgacatc gtgtttgtca cagtcatcgg tcaggtattg
     1801 atgacactag ggatcatggt agtgactttt gcgggccgct ggtttgcttt tccacgtctt
     1861 gttatcttac tttcatttgt tctcggtacg cttttcttaa gtatctatcg tattctcgta
     1921 tataaacttt atttacgcgt aagtcgagat aaaaaggtcg tcattattgg gttagaaaag
     1981 gatgtcgcac cagcaatcca aaattttggt tcaaagaaaa acaacaaaca taaagtcgaa
     2041 gcagtcattc ttgataacta ttatgcaaac atcaaaaata tgattaaaga catcgatatt
     2101 gtctatcttg cttctcatat cgaagaaact gaaaaaatca aaatctatca attagttact
     2161 aaaaatgaaa aaaaattgtt tttgaataca acatttgaaa acttgacatt gatcaatcca
     2221 aatattatga atttcgaaga tgaaagtatt attgaagcat ctggatttag aatccaacct
     2281 gaatatgaac tattcaaacg tttgtttgat atcatcgttt cacttgtttt attagttatt
     2341 gcttcaccat ttatgttatt cacagcaatt ttagtcaaag tgacatcacc gggaccaatc
     2401 atctataaac aagtgcggat cacaaaaaat caaaaggaat tttcaatcta taaattccgc
     2461 tcgatgaccg ccacagccga agcaaaatct ggaccagttt tagctaagag caatgatgca
     2521 cgagtaacgc ctgtaggtaa atttattcga gctgtccgct ttgatgaatt accacaaatc
     2581 ttcaatgtgc tgaaaggcga tatgtcgatc gtaggaccac gtcctgaacg tcctttcttc
     2641 gtcgatcagt tcaatgcaga aaacccttat tactacttac ggcataatgt tcgtgctggt
     2701 atcactgggt atgcacaagt ctatggaaaa tatgtctctg attacaacag taaattacgt
     2761 tttgatttac tttatatcaa aaaatattca ttgatgcttg atttaaagat cttgctgcaa
     2821 accatcaaga tcctatttga taaaatgtcc tctcaaggac ttgaagaaga cgaaaatgtg
     2881 atctcattgg cagaaattgc ttttccgaat gaaaaaatct ttcgctagtt gggaggcgta
     2941 aaaatgaaca atccgaaatt cagcataatc gtaccagtat acaatgcaga agcaactatt
     3001 ggtcgaacga ttgatattct aaaagaattg gattatgaag agtacgaagt cgttttagta
     3061 aatgatggat caacagatca aacacaggaa atcattcaag aactgattga aaatgatgaa
     3121 cgttttgaat tgattactac gaccaatcaa gggcccggat tagcaagaaa cgaaggaata
     3181 agacagtcaa aaggagaata tctcttattc ttcgatgcag atgacacgcc agtaaaaacg
     3241 atcttgtcag attatgatca tttgctagaa aagagtccaa aagcggatct tgttgttagt
     3301 tcgtttgttt ttcgtacaat ggacgaggga agaatcgttt ccgaaaaaga aaacttagtt
     3361 acagaatgtc actatctaac tcacgaagag tttataaaag acatgtatca gttaatgaat
     3421 gaacagttga tgtatgttgt atggaacaaa tgctatcgta gcgatattct gaaaaataac
     3481 caaattctat ttaaaactta taatagttgt gaggatcgaa tatttaactt acattattat
     3541 gaatactgtc atcaagtgat catgaaccca aaagtagaat atatttatga atttgaaggt
     3601 gggaagggga tcactaatca ataccgacct aacaagttcg aaacatttaa agaattctat
     3661 tcattagcaa atactgtaac agacgatatt aataaagacg gtatggcggc gcttctttta
     3721 aaagggacaa cttcagttat tttttctatt tatagtacct ctgcaagaac ttcaaaagag
     3781 aagaaagacg aagcaaagca gatattgagt gatccagtga tccgagaagc caagcaaatt
     3841 gctaagacgg attcttcagc aaaaaaaata acaaagttgc tttacaatat gccagctcca
     3901 ctatttttat ctgctgtaaa agttggaagc ttcgttgaag tgaaaatgcc agggttaatg
     3961 gctgctttga agcgagtata ttagttaatg gaggaaaata taatgaaaag aattttgcat
     4021 tttcagggac gaatgggttt aggtggagca gagtctttca tgatgaattt atatcgtaaa
     4081 attgatcgta caaactatca atttgatttt ttgatttatg aagattatgc agatgttcaa
     4141 gactatcatt cagagattga aagattagga ggccgtattt ttgttgtacc taatcctaag
     4201 aaaaatattt ttaaatattt aatagaagtg aataagttac taaaaaaaga atctttttct
     4261 atagttcaca atcaggttta ttttggcggg ggaattaatt tatggttagc caaaaagaat
     4321 ggaatcagac agagaattgc tcatagccat gcaactgaag atggaaaatc tcaaaatatt
     4381 gtaatgaatg tattgcgtaa atttttaacc aaattgttgt tacaaaatgc aacagactac
     4441 cttgccgtta gtcaagaagc tggtgaaagt ctgtttcaaa accacccctt tgagatagtt
     4501 cataatggta ttgatttaga gctttatagt aaaaattcag aagctaaggt taataagaga
     4561 aaagaattgg atatctcaat gagtactttt gttgtgggaa acattggacg attagagaaa
     4621 caaaaaaatc aatcttattt gttagagatt tttttagaaa tactaaaaat gaattctgat
     4681 tcttacttga ttttagtagg tgaagggtca cttagaaata aactgaaagc caaagcagta
     4741 gaattggata tagagaaaag agtaaaattt ttaggagaaa gaaacgatat tcccgagtta
     4801 ttatctgtga tggatgtttt tgtaatgcct tctctttatg aaggtctgcc aatggttgga
     4861 ttagaagcac aagcagcaaa attaaaatta gtcttagcag atactatttc caaagataca
     4921 aagttaacaa ataatgtgga ttttgtatca ttagatgaaa caccacaaga atgggcaaaa
     4981 aaagtaataa aagagccgta tgctaataaa ataacacaag aactaaagag ttatgatgtt
     5041 tcgtacacta ctgaacaaat ggagagtata tatagtagag atcattaaaa atgttcaaga
     5101 tattttagga gtgattattt ttgaaaatat gttatttgat attggcatac aatgatattg
     5161 gcaacttaaa gagattggtc aatcgattga acgaaaatgc agtttttatt attcatatag
     5221 ataaaaaaac tgatatagaa ccatttattg aagcatttaa atcatatgat aatgttgaat
     5281 ttttatgtaa taaaaaccgt gttaaaattt attggggtgg tttttccatt gtacaagcag
     5341 aaatcaattt agtaaagaga gcattacaaa atgagaaata tttaaaatat gtattattat
     5401 ctggtgctga ttatccaatc aaagataatg agtatattta taattatttt aaaaagaata
     5461 gttcagtgga gtttattcgt ggaatagatc tagatcaaat taaacataaa gaattttatt
     5521 ataaacatat agatgtatat caaaaacatg attatcctag aattaatagg aataacacaa
     5581 ctgcatttaa aatttttaga gctataatta atcgttgtct gaggatgatt aaacttcccc
     5641 caaaaattag acaccataaa tttgatttgt atcatggttc tcaatggtgg gctttgtcga
     5701 aagaatgcct aacagaatta at
//
LOCUS       DAYYNQ010000132         5695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000132 DAYYNQ010000000
VERSION     DAYYNQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.135"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHBJ1_002705"
     CDS             complement(<1..300)
                     /locus_tag="ACHBJ1_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM9431584.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHBJ1_002706"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHBJ1_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHBJ1_002707"
     CDS             complement(1014..1304)
                     /locus_tag="ACHBJ1_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431585.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHBJ1_002708"
     CDS             1706..1942
                     /locus_tag="ACHBJ1_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431586.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHBJ1_002709"
     CDS             1949..3901
                     /locus_tag="ACHBJ1_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9431587.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHBJ1_002710"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHBJ1_002710"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9431588.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHBJ1_002711"
     CDS             complement(4797..5489)
                     /locus_tag="ACHBJ1_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9431589.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1860 a          986 c          911 g         1938 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaac
//
LOCUS       DAYYNQ010000133         5513 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000133 DAYYNQ010000000
VERSION     DAYYNQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.136"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..623)
                     /locus_tag="ACHBJ1_002712"
     CDS             complement(<1..623)
                     /locus_tag="ACHBJ1_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431590.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAY"
     gene            825..1124
                     /locus_tag="ACHBJ1_002713"
     CDS             825..1124
                     /locus_tag="ACHBJ1_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431591.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1389..2264
                     /locus_tag="ACHBJ1_002714"
     CDS             1389..2264
                     /locus_tag="ACHBJ1_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431592.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2261..2479
                     /locus_tag="ACHBJ1_002715"
     CDS             2261..2479
                     /locus_tag="ACHBJ1_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431593.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2466..5093
                     /locus_tag="ACHBJ1_002716"
     CDS             2466..5093
                     /locus_tag="ACHBJ1_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM9431594.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5108..5326
                     /locus_tag="ACHBJ1_002717"
     CDS             5108..5326
                     /locus_tag="ACHBJ1_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431595.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2068 a          696 c         1073 g         1676 t
ORIGIN      
        1 ttataggcta agtccatttc gtctaatagc tgttttaaag aatcaatcat tctttctttt
       61 tcaccgtatt tgaaatcacc atttcttatc ttgtcttgaa gtttactgtt ggctcttttg
      121 aaaaatagct ctttagagat agagtgtctt tggttagcgt actcttcaat cagaggattg
      181 atattattag caagtatctc atttagataa atttcaaaat cttttgagta atattttgta
      241 aatatatttt ttattatagg attcaataac tcatagtctt tatctagcga aaacgtatca
      301 ctatatcttt cttgaatgtc tttaaagact ttgtgaggta tttctgggaa gtccttaata
      361 tatagctcat accctatatc gataaggtat gaagttatat attcttctaa agttccccat
      421 ttaatccagt caattgttga attagtgata gttgataata atttcagctt ttgtggattt
      481 ggtaaattgg tcccacgttc ccagttggat atggtcgcac gtggtgaatt atctattttc
      541 cttccaagtt cttccatagt aaaaccacat gaaaggcgta attgtttgat tcgttttcct
      601 gcttctgaag gatttggctt catctttttt ctccttttta tttttaaaga tagtatatca
      661 tatttataaa ataaaagtat ttaaaagtat ttacttttta tctggagtat gatactttat
      721 tcttataaat acaaataaat acattttttt acgcaatttg tatttatttg tacttataaa
      781 ggtagaggag ttatagaata attaattaaa aaggagaggt ttttgtggaa gcaataaaat
      841 taatgatttc agaagaacag gaggaaatgt ttctaaagca gttttatcaa attgctacaa
      901 aagctattga aaaggctcaa gaaaatgcgg ggatgacaag ggaatatctc aatcaaaaag
      961 aaatggcgga atacttaaat gtgagtgaaa actttttaaa aaatgctgtg agaaatgagg
     1021 gattgcccgt agttgaatta ggggcaagaa aattttattc gaaaaaggca gttaatgagt
     1081 ttatgtttgc tcgaatgaaa agaggggaaa atattggaag atgatgaagt agaaattata
     1141 aaaaaatgct ttaaaaaaag aaaaaaagtg ttcccaaaag tttgcagact gggggaacac
     1201 tcaataataa agtgctgaga aaactttatt tatagctatt atatcaaaaa agaaggtgaa
     1261 aaggtgtttt cttctggaaa tgttgtagtt gacaaactta gagaaataaa ttttgacgga
     1321 aatgtaatcc acacacatgg tacgatcaat taagaaaaaa aacgaaaaaa ggtgttgaga
     1381 agccctattt gaatgcaata gttatcttag cagaaattac ctattggtat agacctaaaa
     1441 aaatttttaa cgatgatggt cagctacttg gttatcaaaa aaagtttctt gaagatattt
     1501 tgcaaaaatc atataagcaa ttaagcaaaa aaacaggatt atcagaacga gctataaaag
     1561 atgcaattgt gtacttagag aaaaagggga ttatcaaaag ggtttttaga aatatttatt
     1621 atgagaaaag agcagttaca ttatcaaatg ttatgtacat tgttttaaat gtacataaat
     1681 tgatagaaat tacttttcct actttggatt gcgaaaaaaa ggagtatatg ccgaaaattc
     1741 gtcagagggg tatcgaaaaa aagagatatg tctctttaaa aaaaggtaga ggtatgtaca
     1801 aatttgtata tacgtataca aagactactt cacagaacat atcaaagact actctcgtta
     1861 tggaagaaga ggatattaat ataaagagga tttacagtaa gattagtgtt ctttatgacg
     1921 caaataacga agatggtcga aaattatttc aagtgtatca ctatttgaga aacgctaatg
     1981 tagatacaga agatatcaga aaaatcattg aatatttatc tgaaaatact cagtatctta
     2041 ttgctgagtg tattataaaa caacatgaag catgcttttt tcaaagaaaa actgaagaag
     2101 ggttatacga ttatgtaaca tattttttga atggacttca gaaaagatat aaaagtcaat
     2161 ttttagaaaa tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc aaagagttac
     2221 ctgaaattac tatgcataat tggttgaaag gagaagaatc atgattaaaa atcgcttaat
     2281 agatcaaatg tggtgtcttg atcaaaatac agatactctc gaattaatcg agagtatctg
     2341 cttcaacaca gtagttctcg atttaagagc tgaaaatgac aattgtgtaa ctcatatcat
     2401 aattaatcaa aaaatggctc agcagctctc tgagagccta agaaaatggg ctgaaggaga
     2461 aaactatgaa agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg ttagcactca
     2521 gtgtacttgt tttaatgatt agcgttgttt ttacaagtaa tattgttgaa gctagcattt
     2581 tcaattggtt tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac
     2641 cttacctaaa aaaatacaat ggagatttcc ttagtcagtt cgggatttgg cttggatggg
     2701 ctgtggtaaa aggcatgttt actgtgactg attcgattca aaatatgatt ccagatgtgt
     2761 tggatttgtt caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagtcataa
     2821 atacaattgt cgttggatta atgatccttt cattgatgtt tgttggctac aagatgatta
     2881 ctggaaaagg aacaatagat ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt
     2941 taattctttt aatgccaaca atgatttcta gcggtattca atttgccaaa atattttaca
     3001 atgatgctac tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag
     3061 gagtaacaga tctggcatat attaataaaa cagatcaata ctcaagcatt gataaaacgg
     3121 aggatcgaaa taagttaaca cgaaaaaatt ttcaacagac agatttaaca caagtgttga
     3181 cggataaagt gattgataaa ttggaaaaag aaaatccagc tgcggataat cttcgctatg
     3241 aattagttga aaattctaat aatgaatttg ttgccacaaa gttttctgat aacttcttat
     3301 caacattttc tgatagcttg aaatcaggtt attatcgata tcaagcaaat ctttggggga
     3361 tttcgattgg gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta
     3421 cagcaatatt agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcgacagata
     3481 ttgaaacagg acaacgttca aaagttgttt tatccgatat tttacaatgt tacttaacag
     3541 ttggatttca agggtttgga ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg
     3601 gacaaggtat ctccacaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg
     3661 tgctgataaa aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag
     3721 aaggctatgg gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat
     3781 caactggatt taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agtggagaag
     3841 gagaagatcg aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa
     3901 ctggaagagc tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag
     3961 ctacaatggc tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg
     4021 ataccaagaa taagttcaaa gaaggtttag atgatggtac agtgtccgct attaataaaa
     4081 atagcaaacc tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca
     4141 tgccaacacg tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta
     4201 tgtctagttc tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc
     4261 ctgtaagtgc aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc
     4321 acggtcaagc taagtctgct gaagaattaa ttaatcagaa gcgacaagaa gctaaataca
     4381 cgcctgaagc aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag
     4441 ctaatacgga cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa
     4501 ataccggatt agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg
     4561 gccgatcagt agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga
     4621 atgttgatgt aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg
     4681 atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa
     4741 gagagaatgt acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga
     4801 atgtacaaag ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc
     4861 aaaaagatag tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa
     4921 agtcagcgaa aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg
     4981 aaaatgataa gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca
     5041 gagatacact taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg
     5101 aattgtcatg aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt
     5161 atgtgaatca gtaaagaacg caacaaatga aaaaatcacg gtcactaatt atattgaact
     5221 gatgattcaa gaagaattaa agatgaaaca tgagataaaa aacaacttga aatattgtta
     5281 tctagaaaaa agtgaagaga taatccatga attaaatggc ttttaaaaat tgtatttgac
     5341 atgaatttac gacaaggaat tagtggggat taagcgatta ttctcttcca ctttagagaa
     5401 ataatgggtt aaaaagccag aatacgtaga gcaatattga caacctataa aggagctatt
     5461 gttgaaaatt gaagtaacaa aaagataaag ggaggtttac atctatcaga tta
//
LOCUS       DAYYNQ010000134         5449 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000134 DAYYNQ010000000
VERSION     DAYYNQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.137"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..1131
                     /locus_tag="ACHBJ1_002718"
     CDS             124..1131
                     /locus_tag="ACHBJ1_002718"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM9431596.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1143..2147
                     /gene="neuB"
                     /locus_tag="ACHBJ1_002719"
     CDS             1143..2147
                     /gene="neuB"
                     /locus_tag="ACHBJ1_002719"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM9431597.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2144..3292
                     /gene="neuC"
                     /locus_tag="ACHBJ1_002720"
     CDS             2144..3292
                     /gene="neuC"
                     /locus_tag="ACHBJ1_002720"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM9431598.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3265..3942
                     /locus_tag="ACHBJ1_002721"
     CDS             3265..3942
                     /locus_tag="ACHBJ1_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM9431599.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3893..5074
                     /locus_tag="ACHBJ1_002722"
     CDS             3893..5074
                     /locus_tag="ACHBJ1_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM9431600.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5087..>5449
                     /locus_tag="ACHBJ1_002723"
     CDS             5087..>5449
                     /locus_tag="ACHBJ1_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9431601.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLK"
BASE COUNT         2023 a          696 c         1058 g         1672 t
ORIGIN      
        1 ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat tagtttagtt
       61 atatattttt taaagattaa gaatcgaatt atagatgact ataattcata ggaggagata
      121 taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat tggaagtgaa
      181 attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag tagaggtgaa
      241 gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag atttttaatt
      301 ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga tattattttt
      361 catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga agctgtaaaa
      421 acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat
      481 tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg tgcgacaaag
      541 ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac agaaacaata
      601 ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat tcctcttttt
      661 agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat gacacgattt
      721 atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt
      781 ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc tcaatcatta
      841 ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa agttgtaggt
      901 atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat tgaacgctca
      961 tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga cgaactgtac
     1021 aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga attaatgagt
     1081 ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta aggagtttta
     1141 tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat ggaagtctag
     1201 atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg gttaaatttc
     1261 aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg
     1321 ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa ttatctcaat
     1381 ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt ttgtctactc
     1441 cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga tttaaaattg
     1501 gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat gggttgccag
     1561 ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta gattgtttca
     1621 tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt tctgaagata
     1681 ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt gggtactccg
     1741 atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct aaggtaattg
     1801 agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct tctttagatc
     1861 ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt ttaggtgatg
     1921 gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga aaaagcctag
     1981 tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt acaataaaac
     2041 gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa aaagttaaaa
     2101 gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa aaatacttat
     2161 agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga atttactaca
     2221 agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata aatatggatt
     2281 tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc aaagtttgtt
     2341 tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa atggtttcac
     2401 aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata gaggagaaat
     2461 gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata ttcacggagg
     2521 cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa tagcatctgt
     2581 tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg aagattcttg
     2641 gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata gattacctag
     2701 ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat acgttttgca
     2761 agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt tagctttgat
     2821 caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata gtgatgctgg
     2881 tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta tttatattac
     2941 gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta tgatcggaaa
     3001 ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa atttaggaac
     3061 cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa atgaaaatga
     3121 aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt
     3181 taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat tgaatcaaat
     3241 attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt aaaatatact
     3301 agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat tgaaacctta
     3361 agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa aatgcaagat
     3421 ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa aaaaaaaatc
     3481 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
     3541 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
     3601 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
     3661 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
     3721 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
     3781 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
     3841 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
     3901 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
     3961 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
     4021 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
     4081 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
     4141 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
     4201 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
     4261 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
     4321 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
     4381 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
     4441 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
     4501 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
     4561 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
     4621 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
     4681 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
     4741 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
     4801 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
     4861 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
     4921 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
     4981 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
     5041 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
     5101 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
     5161 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
     5221 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
     5281 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
     5341 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
     5401 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaag
//
LOCUS       DAYYNQ010000135         5447 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000135 DAYYNQ010000000
VERSION     DAYYNQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.138"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACHBJ1_002724"
     CDS             complement(52..1557)
                     /locus_tag="ACHBJ1_002724"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM9431602.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACHBJ1_002725"
     CDS             complement(1649..2239)
                     /locus_tag="ACHBJ1_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM9431603.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACHBJ1_002726"
     CDS             2418..4064
                     /locus_tag="ACHBJ1_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9431604.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACHBJ1_002727"
     CDS             4113..4832
                     /locus_tag="ACHBJ1_002727"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM9431605.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..>5447
                     /locus_tag="ACHBJ1_002728"
     CDS             5311..>5447
                     /locus_tag="ACHBJ1_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM9431606.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     A"
BASE COUNT         1642 a          996 c         1100 g         1709 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcatt
//
LOCUS       DAYYNQ010000136         5339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000136 DAYYNQ010000000
VERSION     DAYYNQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.139"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..284)
                     /locus_tag="ACHBJ1_002729"
                     /pseudo
     CDS             complement(60..284)
                     /locus_tag="ACHBJ1_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACHBJ1_002730"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACHBJ1_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACHBJ1_002731"
     CDS             2090..3130
                     /locus_tag="ACHBJ1_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM9431607.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3954..4286
                     /gene="traE"
                     /locus_tag="ACHBJ1_002732"
     CDS             3954..4286
                     /gene="traE"
                     /locus_tag="ACHBJ1_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM9431608.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4905..5246
                     /locus_tag="ACHBJ1_002733"
     CDS             4905..5246
                     /locus_tag="ACHBJ1_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM9431609.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1769 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa ataatagcta ttttaataga
     3241 atttataagt taaaatatta tgataaaaat cacaaacgta gccttttacg tcataagtgt
     3301 taaaagaaat aattaatttc aaatatagaa aagggaacgt atgtttgcat actccttttt
     3361 tttgagttat aataatacgc gaagagagat aatttcatta tttaaacaag ctaaaaatct
     3421 tgatattact tcttttcagg caatacgaat attattcgct tacatttttt gtataaattt
     3481 aattgtctgc tatgcttgtg atgaattgat tttcgttttg cacttttgga tgataaattt
     3541 ggacaattga aaatacaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc
     3601 gtcagcgttc atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac
     3661 ggtattaccc gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg
     3721 ctgtgttaag gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat
     3781 aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt
     3841 ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa
     3901 aataacaaga aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat
     3961 atacaggaat tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag
     4021 agaaaatcac gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat
     4081 atataatcca gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc
     4141 aaataatttt aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt
     4201 atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa
     4261 aagggatggg gcgtttgatc ggctgaattg atcattctca taacttgtat tttatcgttt
     4321 ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac ttagagtcac tagaaaaata
     4381 aggagtagac ttgatctatc aacaattccc ttcggggagt gacggggtag gttttaacga
     4441 ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg
     4501 aatttgtgta aataataagc gcagagtaaa caagagggaa tttagaagtt agacaagtta
     4561 gaatagcgtc tagattaatc atgagcagct actaagggat ctagactttg acgcaaactc
     4621 atggaataag gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg
     4681 ctgcagtaca tgtagatact tgcgaggacg ttgattattg gatcaatcga gcatttaccg
     4741 ctgtctacta gggtttctcg tttcggacgc cataaacgag tcgaaatttt ttatgtattt
     4801 ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga ataaagtgaa aaaattgata
     4861 taaaacatag agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc
     4921 cgaatatacg attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag
     4981 gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt agatgtttta gcattagacg
     5041 aggatgattt atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg
     5101 aagatgatga aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat
     5161 tatctgttga agatacttgg tcatatttga gtgattatat tgtcagcttt gaaatcattg
     5221 aatttgtagc ggataaagaa gaataaaaac tggaggaata aattttacag ataaaggctt
     5281 agaaggagtc acgacgatta ataaaaaggt agtattacct taggcattga gtaaggaat
//
LOCUS       DAYYNQ010000137         5319 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000137 DAYYNQ010000000
VERSION     DAYYNQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.140"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..269)
                     /locus_tag="ACHBJ1_002734"
     CDS             complement(<1..269)
                     /locus_tag="ACHBJ1_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431610.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSIL"
     gene            complement(567..1541)
                     /gene="sgrA"
                     /locus_tag="ACHBJ1_002735"
     CDS             complement(567..1541)
                     /gene="sgrA"
                     /locus_tag="ACHBJ1_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM9431611.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1737..2576)
                     /locus_tag="ACHBJ1_002736"
     CDS             complement(1737..2576)
                     /locus_tag="ACHBJ1_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM9431612.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2761..3648
                     /locus_tag="ACHBJ1_002737"
     CDS             2761..3648
                     /locus_tag="ACHBJ1_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM9431613.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4242..4936)
                     /locus_tag="ACHBJ1_002738"
                     /pseudo
     CDS             complement(4242..4936)
                     /locus_tag="ACHBJ1_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(4920..5318)
                     /locus_tag="ACHBJ1_002739"
     CDS             complement(4920..5318)
                     /locus_tag="ACHBJ1_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM9431614.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1699 a         1070 c          782 g         1768 t
ORIGIN      
        1 aaaagtatag aataaacttt actcagtaaa aactgagaag aaaactcgac ttttatcatc
       61 ttgttttctg aaagaaaaga taatttatat tctggatacc tttcgcaaaa cgattctgct
      121 tgttgaatca ccgaattcat cttagccagg gtgtagttta gatctaaagc gatatccttc
      181 accacatata atttctcgtc tctttcataa caatagttca aaaattgata tacttgcctt
      241 tcattgatat caataaattc ttcaaacaaa gtacatactc ctttttatct attatttaga
      301 cttattccga cagctacatg aataacgtac aaatagattc gttactgatt tttcagagat
      361 actaccatgc gcagtgtgca ttgtcaatta ttcttgctct tattttccat ttgtgttcct
      421 gttatttcct gtttctcaaa tatgttagta aacaaaaata gatctggcaa agctacaact
      481 tcgccaggca atacagcact ctcgaatgaa atattcactc aaaaatctat cgttttattc
      541 aattttagat ctgttgcttt tattctttat ttcactcgat ggaagagaga acgaaaatag
      601 cttgctccta caatcaaaat gagtaacaca cccatccatg taattagctt gttcaaggtt
      661 ctactaccag tttctggtaa atcgcttttt ctattatctg tttctttcgc tttttcttga
      721 tgatcaattc cacgagaaac aaaatcaggc tgttttagca cactttttag gttgctccct
      781 gcatttctct tttgttcctt agttggtatg ataggtacca aatcaagttc attatttact
      841 tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac tctcactact catgcttcct
      901 gtgctacttt cactactcgt actctccgta ctgctttcgc tgctcgtgct tcctgtgcta
      961 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctaatttca
     1021 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     1081 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     1141 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     1201 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     1261 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     1321 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     1381 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     1441 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     1501 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     1561 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     1621 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     1681 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     1741 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     1801 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     1861 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     1921 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     1981 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     2041 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     2101 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     2161 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     2221 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     2281 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     2341 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     2401 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     2461 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     2521 acaaaaccaa caaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     2581 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     2641 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     2701 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     2761 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     2821 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     2881 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     2941 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     3001 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     3061 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     3121 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     3181 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     3241 aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca agcttgttca agcttatgga
     3301 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     3361 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     3421 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     3481 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
     3541 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
     3601 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
     3661 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
     3721 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
     3781 ctggatttgt tacaatcatg gggatataat ttaaaaaagt tttttatttc tatcacatga
     3841 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
     3901 ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa
     3961 aatatccctt ctggacttgt tcttcttatt ctgttatact agagaaacac gaaagatatt
     4021 tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga
     4081 gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt
     4141 tagtaatagt cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga
     4201 gttttcgaga aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc
     4261 ttttttagtt caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc
     4321 ctcactgctt tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac
     4381 gttatcgctg agctccagat cgaatggctg tagtgacttg ttcacaattt ttgaaactag
     4441 ctgctaatat ttttgttggt ctttgcgtac gttcgatctc agtccgcaac gaatcaatca
     4501 ctttcatagc atcaatgcct tgattttgca tcctgttata ataaggtgca atataatctg
     4561 ctccattatt aatagctaaa tacccttgta attctgtgta aatagctgta ccggtaatca
     4621 aataaccttc ttttttcaac tttttgatcg cttgaaggcc tgcttgattc accggcacct
     4681 tgataaacgt atcttttccc agttcttcta tgataactcg agcatcctct atcatttctt
     4741 cttccgttct tcctattgtc tgaatatgga taggaatctg tccgattatt tttttgattt
     4801 ctttcatatg cgagaagaag tttgacgctc cttctttttt cagaattgta ggatttgttg
     4861 ttacgcctgt taaattcaaa aactgttgac aagaataaat actttttgta tttgctgtat
     4921 caagcatgat ttccatatag ctttccccca ttctgatcct cttgatcgat tttaatgatt
     4981 gtcccctttc ttaaatgcgg catttcctta tcttctacat acattgttcc tggaagttcc
     5041 ggctcatgag atccatcaaa ttttatcgtg atatgtccta attcattcaa attagtttgg
     5101 acaacatctc ctactgctgt gattacatac atttgtttat caaaacagag cttcatatca
     5161 caagttattt cttttcttac ctgattcaat tcaatcttat aacaataatc tgttaatgtt
     5221 tccggtgctt catctccaaa taaaatcatt attttttctt tcctgaatac ttctgcttct
     5281 ataccaacat ctgttacaac cgtttcaaag atcgtcatc
//
LOCUS       DAYYNQ010000138         5302 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000138 DAYYNQ010000000
VERSION     DAYYNQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.141"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBJ1_002740"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHBJ1_002740"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM9431615.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHBJ1_002741"
     CDS             complement(1297..2133)
                     /locus_tag="ACHBJ1_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431616.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHBJ1_002742"
     CDS             complement(2317..3534)
                     /locus_tag="ACHBJ1_002742"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM9431617.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHBJ1_002743"
     CDS             complement(3683..3844)
                     /locus_tag="ACHBJ1_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431618.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHBJ1_002744"
     tRNA            3917..4000
                     /locus_tag="ACHBJ1_002744"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..5302)
                     /locus_tag="ACHBJ1_002745"
                     /pseudo
     CDS             complement(4240..5302)
                     /locus_tag="ACHBJ1_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1647 a         1080 c          838 g         1737 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ct
//
LOCUS       DAYYNQ010000139         5223 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000139 DAYYNQ010000000
VERSION     DAYYNQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.142"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..540
                     /locus_tag="ACHBJ1_002746"
     CDS             91..540
                     /locus_tag="ACHBJ1_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431619.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            540..1034
                     /locus_tag="ACHBJ1_002747"
     CDS             540..1034
                     /locus_tag="ACHBJ1_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431620.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            1048..1479
                     /locus_tag="ACHBJ1_002748"
     CDS             1048..1479
                     /locus_tag="ACHBJ1_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431621.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            1481..1618
                     /locus_tag="ACHBJ1_002749"
     CDS             1481..1618
                     /locus_tag="ACHBJ1_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM9431622.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            1655..1948
                     /locus_tag="ACHBJ1_002750"
     CDS             1655..1948
                     /locus_tag="ACHBJ1_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431623.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1945..2169
                     /locus_tag="ACHBJ1_002751"
     CDS             1945..2169
                     /locus_tag="ACHBJ1_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM9431624.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2166..3185
                     /locus_tag="ACHBJ1_002752"
     CDS             2166..3185
                     /locus_tag="ACHBJ1_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM9431625.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RADVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3374..3447
                     /locus_tag="ACHBJ1_002753"
     tRNA            3374..3447
                     /locus_tag="ACHBJ1_002753"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3408..3410,aa:Ile,seq:cat)
     gene            complement(3962..4261)
                     /locus_tag="ACHBJ1_002754"
     CDS             complement(3962..4261)
                     /locus_tag="ACHBJ1_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9431626.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(4267..4497)
                     /locus_tag="ACHBJ1_002755"
     CDS             complement(4267..4497)
                     /locus_tag="ACHBJ1_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431627.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(4756..4869)
                     /locus_tag="ACHBJ1_002756"
                     /pseudo
     CDS             complement(4756..4869)
                     /locus_tag="ACHBJ1_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1836 a          843 c          994 g         1550 t
ORIGIN      
        1 aaattccaag aagctgtaaa tcaagcagaa aattacacaa acgaaagaat tccaaatttt
       61 acttttgaaa aaattgggga ggtatagaga atgacagata tcgtaaaaat aaaacaaagc
      121 aatgtacagg tttatccaca aactcattgg aatgctatag aaggcaaacc aacaacggtg
      181 aagggagata aaggcgatcc tggccaagca gcaacaatta ccataggaac cgtttctagt
      241 ggttcaaccg cttctgttac gaatgtagga acatcatctg cagctagatt taattttgtt
      301 ttaccaaagg gagataaagg tgatcctgga ataaatgcca caactacagc tgtagctaca
      361 actacagcaa atgggttgat gtcgtccaca gataaaacta agttggatgg aattgcagct
      421 ggagcacaga aaaatccagg taatgctaca actacgacag caggtttaat gtcagcaacc
      481 gataaagtga aacttgatgg attagcgaat attacatttg agaaagtggg gacggtttaa
      541 tggctgatat tgttcaatta aaagaagacg gagttgctaa atacttaaaa acgcatgtag
      601 aagctattga tggtaaagaa gcattggtgc agacagatgg agaccaagcc attgcaggac
      661 ataagaattt ttcaggttct gtaacaatca ataataaacg tgttttgacg actgatgata
      721 acagatatga agtggtaaat ctggttgtca ctaacggtaa tacagggaca gcaaagcttt
      781 atcgtgaagg aaaaacagtt tctatatatt ttgtggcttt aaacggaaaa agtagtggcg
      841 ggaatgattc ggttatttta actgttccag aaggctatcg gacaccaatt agttttgaac
      901 aactggttgg gtcaatagac cgttctactt tgaacagtgc tcaactatct attggcgcag
      961 acggagccat taaatggaga agaaattcaa gttatggatc agcttattca tttgttatca
     1021 cttattcaat ttaagggagg aaatctaatg aaagtagttt acaaatcaat caagccttac
     1081 ggattcgagc aaatcatttt gaacaatcaa gaaaatatcc ctgaaaactg tacagagatt
     1141 aaaccaccag ttcctaactg gagaccaaga tttgattttg ataaaaaaca gtgggttgag
     1201 ttagctactg aagaagagaa aaaagggaat gctgttgata gcgtagatga attggctaat
     1261 attaaggcat tatacgaaac gctgaaggca gaaaatgatg aattgaaaca gctaaattct
     1321 aaagcaatgc taaacaatgt agcaatcaag caagaaaatg tcttattgaa agaaaagtca
     1381 gaaagtttag cgcagttgaa ttcaaaaacg atgcttgctt ctgtacaaaa taccaaggaa
     1441 atcgaagaaa tcaaaaaaca attacaaggt gggaagtaac atgtattcat atgaagatat
     1501 caaactaatg tatgactggg gctgtttcac taacgaacaa gtaatggttt tcgttccatt
     1561 atgcattaca gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt
     1621 gatgcgtttt tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat
     1681 gatagcagga tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc
     1741 tgcatctttc tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct
     1801 gactaaagat ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa
     1861 acacgatgaa agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa
     1921 aactttgttt agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc
     1981 gcactggtaa ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat
     2041 tggcagtaca cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt
     2101 gtgttgggtg tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga
     2161 aacaaatgaa aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat
     2221 tgccaattaa cggatttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag
     2281 gtagctcaca agtagcaaat aatcagtata ttttactgca tgaaacggct aatgaaacag
     2341 caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt
     2401 atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat gtacagtacg
     2461 gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa cacacacatg
     2521 ataaagcaac gtttgagaaa aactacaagg catacgttga attggctaga gattcagcaa
     2581 tgaaatatgg tattccatta acgttggaca ctccttataa ccaaccggga atcaaatcgc
     2641 atttatgggt aacacaaaac atctggggcg atcatacaga tccttacggt tatctttctg
     2701 aaatgggcgt aagtaaagaa aaattagcat atgatttagc tcatggattt accgatgaaa
     2761 atccaacaac ttctgaaaac aagcctgtca ttgatccaac acgagctggt gcagctaatc
     2821 ctacactgac agatggaacg aattacgccc acattgatca gtttggagaa atcgaaaatg
     2881 caaatttgca tgtagctgga tggcacattg ctaactataa atacgagtat atcttcatta
     2941 tggattacaa tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagagcag
     3001 atgtaaatca agcttataat acttctggaa atgttggcta tcatgtatct tttaacatgc
     3061 gtaacttccc taataagaaa gtctatgtca tgatgcgggc aacgaatgat ccagagggaa
     3121 acactaaagg cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac
     3181 gataaaaata gctcctcgtt gaggagcatt acataactat attgacaact ataaaaatca
     3241 ttcgataaaa tagtgatgtt atcgcatatc ttcactatca cccataatag tcacactcca
     3301 agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt atggctttat
     3361 gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg
     3421 tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga
     3481 attttttgaa gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc
     3541 ttttttatgt atccttttat ggattaatga aaggatgttt cacatagtta tatttctgta
     3601 tatttgaaaa gttttacttt gatttttaaa tagaaagaca tttgggttat attgtgagat
     3661 aataataaag aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc
     3721 tttaaactct tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga
     3781 gaaaaaaggc ttataattgg agtggtagtt aattagtgac ttatttttga ttttatagca
     3841 ctgatactat aaaatataga tatcatcata ttacacaatc ttaatactaa cttaaaaaat
     3901 atctcctttc ataagtatgg tgataaaatc cgttccgggc taccttttta ggtagcctac
     3961 tttaatcttt gtatctttct ggatcaacga aagtatactt tatatagtca taacgccgat
     4021 gatcgcttcg agcgtctagc acatcagtca cgatatcaaa caaaaaatat acgtctttct
     4081 tcattctagt tttcgcagca gggattttaa agtagttctt attagaatag tagagattaa
     4141 ttaataagct atcttcgatt gctaaaaaga aaacttctga atcccacacc ttataaaaat
     4201 ctttgacaaa tctattcgaa ggatcaaatt taaaccataa ttgcgtctca ttaaaaagca
     4261 taaccattac tccgatctat ttttaaactt agtctctacc tctaatatat atcgagtttt
     4321 tattttgcct tcagagaata ctgtttcttt ttttgcagtt acaggttgtt tattttcgga
     4381 aaaagctaat atagctaaaa ttgaaacatc catctggaat ttgtcttttt cgttgctttg
     4441 ctcataaaaa tctgcatatt catcactaat attttttcta ataaattctt ccatcataaa
     4501 gatcacctcg aaaagagtat acgaacaaac gttctttttg taaaggtgga atttattata
     4561 ctaaataaat aggtgaacaa gtacttgtgc caaattatgt gccaaaaaaa atcgaattta
     4621 ataaaacaca aacaaaaaga aatcctatta tgataacatt tcttataata acaaacacaa
     4681 cagaagacgt gtaatagtta gtcaggaacg tacaaataac ccctgtatcc tttgcggtac
     4741 agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt
     4801 cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg tatgtcttag
     4861 tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag aaaaacttac
     4921 taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac gatttcgatt
     4981 ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc ggtataactt
     5041 gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta tttttagtaa
     5101 tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa aaaacggtgg
     5161 ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt ctatgcttta
     5221 tat
//
LOCUS       DAYYNQ010000140         5206 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000140 DAYYNQ010000000
VERSION     DAYYNQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.143"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="ACHBJ1_002757"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="ACHBJ1_002757"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM9431628.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBJ1_002758"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="ACHBJ1_002758"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM9431629.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="ACHBJ1_002759"
     CDS             2594..3379
                     /locus_tag="ACHBJ1_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9431630.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="ACHBJ1_002760"
     CDS             3392..4243
                     /locus_tag="ACHBJ1_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM9431631.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="ACHBJ1_002761"
     CDS             complement(4367..4819)
                     /locus_tag="ACHBJ1_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431632.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1770 a          844 c         1072 g         1520 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaa
//
LOCUS       DAYYNQ010000141         5168 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000141 DAYYNQ010000000
VERSION     DAYYNQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.144"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1793)
                     /locus_tag="ACHBJ1_002762"
     CDS             complement(27..1793)
                     /locus_tag="ACHBJ1_002762"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431633.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1753..3507)
                     /locus_tag="ACHBJ1_002763"
     CDS             complement(1753..3507)
                     /locus_tag="ACHBJ1_002763"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431634.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3617..4186)
                     /locus_tag="ACHBJ1_002764"
     CDS             complement(3617..4186)
                     /locus_tag="ACHBJ1_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM9431635.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4204..>5168)
                     /locus_tag="ACHBJ1_002765"
     CDS             complement(4204..>5168)
                     /locus_tag="ACHBJ1_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM9431636.1"
                     /translation="IGCSLSLNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIV
                     IAEHRFYYLKELIDQVLFVKNRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKV
                     SADVLKVEEITYENILKDINFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKII
                     ENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLP
                     FDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIAT
                     HDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1721 a          970 c          700 g         1777 t
ORIGIN      
        1 tgtaggttaa tttaaagcct atttcatcat cgtgattgag cttctttttc atagacgaat
       61 ttctcagata gtttagtttt ctttatgaga tttttgtata tttctgacct tcgatacaac
      121 tcattatgag taccttgatc aactagtttt ccttgatcca tcactacaat tttatttacc
      181 ttttcaatag acttcaagcg atgcgatata attagtactg tcttatcttg tattaaacga
      241 tttaaacttt cctgaatacg tttttcattt tccgcatcta aactggcagt aatttcatct
      301 aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct
      361 cctccagaaa gtgaacttcc gttttctccg accaatgtat gataaccgtc aggtagtttt
      421 tcaataaagt cttcgcagtt agctaaacga gctgcttttt tcacttcctc atctgacgct
      481 tgactatttc caatacgtat attttccaaa atacttgtat taaacaaagt tacctcttga
      541 aaaacaatag cgattttgct aaacaaagaa gctggagata cacgtttaat atcataccca
      601 tcaattaaaa tacagccttc atcataatca tacagtctag acactaattt caagatacta
      661 gttttcccgg aaccactagc acctactaac gcagtcactt ctccttgttt agccgtaaaa
      721 gagatatgat ctaaaattgg ggtattgtta tcatatgaga aagagacatc tcgcaactca
      781 atgtcaaaag atttcagagg actatctgat ccctcttgga tactggtttc cttcatagct
      841 ctaattcgct gtattttagg agctaaataa aatatttcaa gcactgcttc tttcatagaa
      901 tcgaaggaat cctttatttt tattgcggct agtaagtaac caacgacgta gagtatagtc
      961 acttcacccg ttaacagtaa gtgaacacct actagaatga ctactgctaa actaacatat
     1021 gagaaaatag aggataaggc catgactgaa aaagtactta attctacctt taaatgaatc
     1081 ctttcactct cttccatttt tttgaaaagt ctgtcttgaa cttttttaga tagattaaag
     1141 ctattaatct cttgctgcaa ttctatagtt tcttgaaatt cttccgagtt ctctctcaaa
     1201 gtatcgtaat atttagtatt ggctttcgtt tgggatttct ttgataacaa gattaagaca
     1261 aaactaaata acgttggcaa aataacagct aatcccattt tgacattacc aaccaaaagc
     1321 atcactgaaa taaaagggaa aaacagagcc ataccgccgg ctttaggtat tgcatgactc
     1381 atcgcatgct caataccttc aacgtcagac atgatagttt gagataaatc tgataaatta
     1441 tgttttgaaa aataggataa tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa
     1501 tgcgcacttt ctttataagt actattatat aagttttcat actccctatc taacaaccaa
     1561 tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa gccaacgggg ctttgtattt
     1621 tctaaaacta attcgtctat cagcatcatt ataatgaatg ctggtaccat attaatgcag
     1681 tatacaaaaa aactagaaat agaagcttta gttaaagctt ctgcgccctg atcacttaaa
     1741 gcaaatttgt ttttataaaa acttttcatt gtcaagcctc caatcatttg cattctcata
     1801 attagtgacc aattgtttat acacttgatg tttagccatt aattcattat gatttccacg
     1861 ttctactatt ttcccttctg acaagacaag aatttcatct acagatttaa tgctggataa
     1921 tcgatgagct atcataataa ctgttttttc tttcatcaga tgttgaaaag ccttttgtag
     1981 ttcatactca ttatctggat caatagatgc actggcttca tccataataa caatctttgc
     2041 atcttttaaa attgctctgg caatagctat acgttgtttt tctcctcctg acagataaac
     2101 tcctttacta ccaattaagg tatgctctct ttcgggaaat ttttctaaaa tcgattcgca
     2161 accggctaat cttagcgcgt caaatacttt acttgctggc gcttgaggat ttgctataga
     2221 aacgttatcg taaatactct gattgaatag tttcgtatct tgaaagacaa aagcaattgt
     2281 gttaataatt gtgtcttttg aatagtgatt taaaggctta ccaccaattt taatctgtcc
     2341 cttatccaca ttgtagaaac ctgaaattag tttagctatc gtactctttc cgcttcctga
     2401 ttttcctact aatgcataaa ttcttttttg ttctaaaact aagttgaaat tttctaaaac
     2461 acactcttct ttgtatgaaa agctgacatt ttcaaatgca atgtcacaat ttttaatctg
     2521 tgtctcgttc ccatatttta gttggtcttg cttcatttct aaataaaggg agtctagtgt
     2581 ttcgactgca tatcttgctt caaacaagtg catacttaca tacattattc tcatgaaaga
     2641 aacaaaaata attccactta aaaaaaacaa catcaacaag tcaacagcta atatcttggg
     2701 agtggtaaga gaagttataa aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa
     2761 aaatagccat tgaaaaagaa caaaaggtgt tttcccactc aaagaatact tatatgcaaa
     2821 cttagcataa tcgttaattg ctttattcaa ggctttaaat gatgttactt tagcaccaaa
     2881 tatctttata acttgaattc cgcggatata ttctaccgtt tcagagctta acacatctaa
     2941 cgattcttga tatttttgaa taaaatttgt attctctgtc attttcttta acaagaatac
     3001 accaattact aacatggcga ttagaaccaa tccaatttta aagttaatga aaaagcctaa
     3061 ggcaactgat aaaacaggtg ttactatggc ttgagcacta tctggaatca agtgagcaat
     3121 tgccatatgt gttttagccg cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt
     3181 taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta attccctttt ttctcaaatt
     3241 agtctctaag cgaaacccaa ggatatgaga aaataagcca gaaaaaatat aaaataaagc
     3301 tcctaaagtt aaccaagcaa cagtttgaac cgcataactc ttagctaaat attcatctcc
     3361 tacaataatt agagactgaa gaaatttgta aatactgtaa tatccataga ctgttaatac
     3421 gacagaaaaa tcagaaaata aagtggctaa ccatccgtag tatttcattt tagggacata
     3481 actaaataat tttttgtata ctttcatttt ttcctccccc gcattcaatt tcttctctta
     3541 ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat tagagatgta caaacagata
     3601 aaaggtaaac tacgttctat tttttgtatt tttttaagac ttttcttcca atcaatacac
     3661 caatcataga taaacttgct acaactaaca ctgaaatgat tccccatata cctaccgcat
     3721 agtggatcat tgcatccatt gttgcagcag attgtccttt atctatccaa tcttgacgaa
     3781 aacgatctgt aaagaaccat ataggaatcc actgtccggc agcactgcct aattgcatta
     3841 gagcccaaga cgcaacttga ggaattgctc gatcatatcc aaatacagat agaataatat
     3901 cagctaagat cgccatacta atcgaactga taaaccaagg taagtacgcc ccgcccatta
     3961 acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa aattcctttt ctagggtagt
     4021 tatagctaat ataaacaaat atgattccag ataataatcc aaaaatcccg ggggatatgc
     4081 tgtgaccaaa cgccccacca atactactaa ccattattga aaaaaagtaa attactaggg
     4141 aaataactgt agctaaacca ataaaaatat aatcttttgt tttcatatat attctcctca
     4201 attctatttc gttatgtcat taaaaatctg ttctatcaaa gttgaatctt ctactctaag
     4261 ttttatatct ttttttattc caccattttt taaataaata attctattgc atgttttata
     4321 aagaaattcc ggatcatgag tagctataac tactgcaccc ttttctttta agtctttaat
     4381 aaggtcagca atcctttcca tattaatata atccaaccca cttgtaggtt catcaaatag
     4441 aaataaatta gtttttgaca atgaagctat agaaaccaat aatctttgtt tttctccacc
     4501 ggataaatca aacggtaatt gatctttcat tttataaagt tccatttttt ttaaaatttt
     4561 ttcttgctga tctttactaa ctatttcatt ccccagggct aactcgctcc tcactgattc
     4621 tgtgaaaaat tggtaatcca tttcttgcat tactaaaaac ggcgatgaca aatttttttt
     4681 attttcgatt atctttcctt tggtagggcg aataatcttc atgagtattc ttagtaaggt
     4741 tgttttcccc acaccgtttt tccctaccaa accaataaca tctccaactt tgacatcaaa
     4801 atttatgtct tttagaatat tttcataagt aatttcttca acttttaata catctgcact
     4861 taccttttcg atatagggaa cttcaatcga actattaaaa atgtcaaatt tccttaatgc
     4921 gttattgttt tcagttttta acaactgaat agttgtagtt tttacagttc tatttttaac
     4981 aaatagaact tgatcaatta attctttcaa atagtaaaaa cgatgttcag ctataacaat
     5041 agtaaatcct ttatttttta aatcacaaag taagtcagca agtttcttag tcgctttgaa
     5101 gtccaaattt gaagacggtt catccaatat aattaattta gagtttagcg ataaactaca
     5161 tccaatag
//
LOCUS       DAYYNQ010000142         4972 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000142 DAYYNQ010000000
VERSION     DAYYNQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.145"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..572
                     /locus_tag="ACHBJ1_002766"
     CDS             87..572
                     /locus_tag="ACHBJ1_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM9431637.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(676..1269)
                     /gene="clpP"
                     /locus_tag="ACHBJ1_002767"
     CDS             complement(676..1269)
                     /gene="clpP"
                     /locus_tag="ACHBJ1_002767"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM9431638.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            1449..1976
                     /locus_tag="ACHBJ1_002768"
     CDS             1449..1976
                     /locus_tag="ACHBJ1_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431639.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            2067..2804
                     /locus_tag="ACHBJ1_002769"
     CDS             2067..2804
                     /locus_tag="ACHBJ1_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM9431640.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            2808..3725
                     /gene="miaA"
                     /locus_tag="ACHBJ1_002770"
     CDS             2808..3725
                     /gene="miaA"
                     /locus_tag="ACHBJ1_002770"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM9431641.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            3727..4956
                     /gene="hflX"
                     /locus_tag="ACHBJ1_002771"
     CDS             3727..4956
                     /gene="hflX"
                     /locus_tag="ACHBJ1_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM9431642.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         1691 a          850 c         1111 g         1320 t
ORIGIN      
        1 tttttttctt atgaaataaa tatatgctat gataccttta ttcaatagga ttagtttcta
       61 caaaaaatga aaggagaaaa ataaatatgg aacggtttat agcatcaaat gccacagtga
      121 ttggtgatgt gacattgtca gaagatgtga cgatctggta tcaagctgtt ttacgcgggg
      181 acagtaattg gataaaaata ggacaaagaa cgaatatcca agatggtaca atcatccatg
      241 tcgatcatga tgcacctgta gatattgcgg agaatgtgac ggtaggacat cagtgcatgc
      301 tacatggctg tacaatcgaa aaaggggcgc tgatcgggat ggggaccacg atattgaatc
      361 atgcggttat tggtgagaat agtttgattg gtgctggatc attagtaaca gaaggaaaag
      421 ttattcctcc taatgtcttg gcttttggtc gcccagctaa agtgatccgt cctttgacaa
      481 aagaagaaat acaaaaaaac aaagaaaaca tccagcatta tgtcgagata ggtcggatgc
      541 atgcgcaagg aaaattcaag gagcaaagat aggcagtagc atccataaaa taaaaaagga
      601 tggtcagttt gagttgacca tcctttttta tttggtttag ataagagagc ttaaaaaagc
      661 ttcctttgaa acagattaat ttaatgaaga actgttttcc atgacttcat cgatcaatcc
      721 atatgctttt gcttgttcag ctgtcatata gttgtcacga tctgtatctt tttcaatcac
      781 ttcaagcggt tgtccagtgc gttcagctaa aattttgttt aagcgttcgc gtgtttgcaa
      841 aatatggcgt gccgcaattt cgatttcagt tgcttgccct tgagcgccgc caagaggttg
      901 atggatcatg atctcagcat ttggtaaagc aaatcgttta ccttttgtac cagctgtcag
      961 caagaagctt cccattgaag cagccatacc cataacaatt gtttggacat ctgctttgac
     1021 aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact gaaccaccag gagaattgat
     1081 ataaagatag atatcttttt cagaatcttg agcgtctaag aaaagcaatt gggcgatgat
     1141 cgaattagca agatcatctg ttacttggcc gcttaacatg atgatacggt ctttcaataa
     1201 acgtgagtag atatcgtaag ctctttcacc tcgtgaagat tgttcaatga ctgtaggaat
     1261 taaattcata atagagttcc tccttgattt cgtttagaaa tagtgtacca taatttagtt
     1321 tctaatacct aaaatgtagt atacattttt ggtcaaaaaa ggtcaaataa aaaacttagg
     1381 caaattagtc gcataaaccc gctttatggt atgataagca gagaaacaaa acaaggagga
     1441 acaacagatt gatagttggt attactggcc ttatcggagt gctgacaggt gctgtagttg
     1501 tctatatcgg catgatactg caaatgcgtc acttggaaaa taaagaacag cgtagacgtg
     1561 aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca attaaataaa aagatcaacg
     1621 agatcttgca aaaacggaca acgctgatgg aagaatatgt cagctttgat gcatttgatg
     1681 attgttatat cacgattgac gattttgcgt atctccaatc attttcagcg caaaatagtt
     1741 tttatctgcc aaattatatt ttagacgagc tatttaagaa tatctcccat cgaaaagtga
     1801 ttttatctcc agatgaaaca atgaagattg gtggatatgc ttataaaggc ggacgcgtga
     1861 ttttagagaa ttttacggat agtctgactg aaatgatcaa tgaaaaaaaa gctcagttga
     1921 agactatgac gcgccagccg attggtttct tctcgaaaga tgatgtttgg agctgatatt
     1981 ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt tctttttttc ttaatgatac
     2041 aatagaactg aacggagtga agaaagatgt tacaaatcta tgcccatcgc ggaagcagcg
     2101 gcacccaccc agaaaatacg cttcctgcat ttgcagaagc tgtacgagta ggtgcagatg
     2161 gtatcgaact agatgtccat ctaagcaaag atggttattt gatcgttatg cacgacgaag
     2221 aagtggaccg gacgactaac ggtaaagggc tgattcgaga aaaaacattg gaagaactca
     2281 aaaaattgaa tgcagggggc aaattcagca aagaatttcc agcagctaaa gttcctgaat
     2341 tgagagaagt gttaggactg ctcctccaaa aaaattaccg agggatgcta atgattgaaa
     2401 ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa attagccgaa acattaaaaa
     2461 gtagagattg gccttttcaa catgcatatt gcagctttca tttggattct ttggaaaaaa
     2521 tcgcattact ggaaccggag atacagctgg acttattgat gagtacttct gataaaaaga
     2581 tccaactagc agcagaaacc ccttatatag aaggagtaca tccaaaaaat acttggttgt
     2641 tcgaacatcc agtccaggca gctgattttc caaaagcaat tcgtgcatgg acgattaacg
     2701 atgaagaaga aatggccaaa tgtcaagaac tcaaaataga tggaataata actgattttc
     2761 cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag gtaaaatatg gaaaaagtat
     2821 tagtgatcgt cggtccgaca gcagtcggta aaacagcgct aagtgtggaa ctcgcacaac
     2881 tatttggagg agagatcatc agtggtgatt cattgcaaat ctataaaaat ctggatatcg
     2941 gtacagcaaa aatcaaagaa gaagagatga ataatgttcc gcatcatttg attgatgtga
     3001 tcgaacctac tcaacgttac tcagccgctg attttcagca ttcaggtcga caattgatta
     3061 ccgagattgc gaaccgaggg cacctaccta tcatagctgg tggtacgggt ctctatatcc
     3121 aatcccttct atatgattat cagttaggag cgacagaaga agcgaatcct gagattagaa
     3181 aaaagtatga aacgttagct gaaacaattg gttcccaagc attatggaat cagttgaaag
     3241 aaaaagaccc ggcagctgct gagaaaatcc actggaacaa tcaacgcaaa gtgataagag
     3301 ctctggaagt tttagagacg acaggacata gtatcacggc acctaaagaa gagccaaagc
     3361 aattgtatga ttacctcatg attggattag atacgaagcg tagtattctt tatgaacgaa
     3421 tcaatcaaag agtcgatttg atgctagcag aaggtttagt agcagaagcg cgtaaggtgt
     3481 atgagcttgg agaagtccag gccagtcaag gtattggata taaagaattt ttcccttatt
     3541 ttgaagggaa agaaacattg gaagaagcaa cagaacaagt caaactacat tcgagaagat
     3601 acgcgaaacg tcagttgacg tggtttcgta accgtttgga tgcacagtgg tttgatctta
     3661 taagtgagcc aaatcaaaaa agtcagattg agcaaacaat caaaaaatgg ctggaggcgg
     3721 aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa ccgaagcaaa ccagcgttac
     3781 ttttcagagt caatggaaga gctagttcag ctgacgaata ccgcatcagg agaagtcgta
     3841 ttcacaatta cacaaaagag accacaagtg gatcggcaaa cgattatcgg taaagggaaa
     3901 ttacaagaat tagttcaaca agcggatgcg catgaagcgg atttgatcat ctttaatcat
     3961 gagctaactc cgagacagag ccaactgatt accgatgctg ttggtcttcc ggtaattgat
     4021 cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc gttcgaaaga agggaagctg
     4081 caggttgaac ttgctcagtt agaatacctg ctgcctcgtt tagtagggca agggaaaaca
     4141 ttatctcgtc ttggcggtgg gatcggaaca agagggccag gtgaaacaaa gttagaaaca
     4201 gaccgccgac atatacggaa taaaattttt gctgttaaga aagaattgaa agaagtagaa
     4261 gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg agatttttca gattggactt
     4321 attggctaca cgaatgccgg gaagtcaact atcttgaatc tattgacaca agcggatact
     4381 tattcgaaag atcaattgtt tgcaacgtta gatccattga ctaagagatg gcgttttgct
     4441 gaaggatttg agattactgt aacggatacc gtcgggttca tccaagacct gcctacgcaa
     4501 ctgatcgatg ctttccattc tacattagaa gaaagtcaaa atatggatct tttgctacat
     4561 gtagtcgatg ctagttctcc agatcgtatt ttacaggagc agactgtctt aaaattgatg
     4621 gatgaactga atatgaaaga aatgccgata ctgactgttt acaataaggc agaccagatc
     4681 gacccagcga tgttcacgcc tacgctattc ccaaatgtat tgatttctac tcagacgaaa
     4741 gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc tgatggaact aatgacccct
     4801 tatacacttt ctgtgccaag caacgaagga cagaaattaa gcgagttgag aagacaaaca
     4861 atggttttgg acgaagcgta catggaagaa acaaacgaat atgaagttaa agggtttgca
     4921 aaaaccaatt caaaatggtt aagaaaaaac caataaaatt ttattttttt aa
//
LOCUS       DAYYNQ010000143         4961 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000143 DAYYNQ010000000
VERSION     DAYYNQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.146"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(139..1338)
                     /locus_tag="ACHBJ1_002772"
     CDS             complement(139..1338)
                     /locus_tag="ACHBJ1_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM9431643.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1468..2844)
                     /locus_tag="ACHBJ1_002773"
     CDS             complement(1468..2844)
                     /locus_tag="ACHBJ1_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431644.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2844..3500)
                     /locus_tag="ACHBJ1_002774"
     CDS             complement(2844..3500)
                     /locus_tag="ACHBJ1_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM9431645.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3510..4787)
                     /locus_tag="ACHBJ1_002775"
     CDS             complement(3510..4787)
                     /locus_tag="ACHBJ1_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM9431646.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4806..>4961)
                     /locus_tag="ACHBJ1_002776"
     CDS             complement(4806..>4961)
                     /locus_tag="ACHBJ1_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM9431647.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1556 a          903 c          608 g         1894 t
ORIGIN      
        1 tcggagtaag tggcttcgac gattggataa ataaattcaa aatcaagcgc ggcatcaatc
       61 tttcgaacga gatgatcttg aggaactaga tcatcaatcg taagcatcgc aaattgattt
      121 ctaccattcg tattgttttt agacatcata gctatcactc cttgcattcg ttttcataaa
      181 tctattataa aagaaaagac tgtagacatc atccataaaa tggactttgt ctacagtctg
      241 cgcattctca ttagagaaca ctcccttttt tgtgactttt ctgaagctag agtgaatact
      301 ttcaactgca ttagtagtgt acatgatttt tctaatggcg ctgccataat caaagagttg
      361 ttcaacatga gaaaagtttc gtttgcatac atctactgca ccagaataat gagaccaccg
      421 attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa gaagaagcac tgtagaattt
      481 tttcatgtct cgacagactt ccttataatc cttacttggg aggtatcgta acgcatttcg
      541 aacaagatga acaatacagc gctgcacaat gacagaagga aaaattgctt ttgcgccttc
      601 tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca tcttctacac cacgtacttt
      661 cagttcatca aatacttgca tccagcgatt tttagattct gtttgattga gccataggcc
      721 taaaatctcc ttgtttcctt taagatcata gccaagaatg gtgtacacag catattcttt
      781 agcttcatag ttttcgcgta aagtgacgta catacaatca acgaatagaa atgcataaca
      841 cttggctaat ggccgagctt gccattcttc caattcagga aggacggcat ccgtgatatc
      901 agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct tcaacggttg cggcgatatc
      961 gcattgactc attcctcgtg catacatgga aagaacctta ccttcgatgt cggagacatc
     1021 tcgttttcta ttaggaatca actcaggttc aaaagaagcc tcccggtctc tgggaacatc
     1081 aatgtttact ttaccaaagc tcgttttaag cgtcttattg ccataaccgt ttcgtcgatt
     1141 gtcatgtttc tttggctctt tagaatgggc gtcataccct aaatggttat tcaattctcc
     1201 ttgaagcatt ttttcaaata agggaccaaa cacatctttt aaaacatctt gcatatcctc
     1261 tacagattcg ggttgataag tattcaaaat ggattcagct aacttctcag catcaggatt
     1321 tcttttcttt ctagccatcg tgtaatcacc tcttgatctt attttagaaa aataaaaacc
     1381 gtgaagcaac cacccgccta ggattgatgg ttacttacac ggtttatatt acactctcat
     1441 ttttggtgct aaaaagcacc gaaaatttta attattgtca attaataatt cttttggatt
     1501 ccttcttaat atattgaatg aagaaataat caatgataca agaagtacta aactcataaa
     1561 tataactaca taaatcatga atttaggtaa tacattgata tctaaacttg ataatgtctt
     1621 gttaaatcct tcagcttctg ccccaccacc taattggcta gatgcagatt gtttagctat
     1681 ttgtttagct atatcaccag taaccttatt caatatatta ttcccaagct tgtcagctgt
     1741 atattgagct aagaaatagg aaccaacgaa tgcggggatt gatataaata ccatctcaat
     1801 taggaattga ccaaaaattt ctaattttga tatacctaat gatagtaata ctgctatttc
     1861 tttctttcta gcattcatcc ataagaataa taataatgaa actacaactc ctgcaaatat
     1921 taatgagcca aaaaataatt tatttgaaat tgagtaaata cctgatatag attgttgtaa
     1981 tgcagggtaa tttgatgaac ttttaatcaa attatattct ctccaattta tatctaattt
     2041 cccaagatct tttattacac tatcaagatt cttatcacct tttacaaaga atgttgcatc
     2101 ttggtataca gctgtatctt cggtattacc ataaacttta gcagctgtat gaatatcagt
     2161 gattaatgta ttttcgtata gttcttgtgc tgcacttact ccaccgctat tatgcccatc
     2221 gaatagacct ttaatttcta cttctactgt ttcatctgca cctttctcat tatcagcatc
     2281 aaataaatta gattttattt ttatcttatc tccaacttta aggttatttt tttcagctaa
     2341 atctttatgc attaagattt tatttttatc ttcattttct aaatgctttc cttctactaa
     2401 tttatatgct tctgatacaa attttgtttc ttttgttgag tcgttaaccc cagttaacat
     2461 aactgttctt ttaaaattct ttgctctttc aggtgattga ttcgcaagag tctcttgagt
     2521 ttcaataata tcataatcaa ctaaatctgc aacactgttt attcttttta cataggagtc
     2581 tatactatct gtttgagata tctttttaat atcttcacct tttacatttc ccccacctct
     2641 aggtgttcct ggatttactt gtctatttat ctccatagaa aaactatttg ttatattagc
     2701 aaatgtttct tttgaagctc tgtccgtagc atctttaata gataaactaa tgagacttaa
     2761 agttgacata gataaaataa ctaatatgat aattaatgat tttaaacttt ttctagttac
     2821 ataagcaaat gcatttttta tcattattca acctccaaat tcattttatt tacttttttc
     2881 aactttttac cacttagttc taaaatgata tccgcagaat ctgctacttc cttactatgt
     2941 gttacaacta ttacacattt atttctatct ttagctaatg tctttaatat attaattatt
     3001 tctccagcag taacactgtc tagattacca gtaggctcat cagctagtat tattggagca
     3061 tctgatacca atgctctagc aatagctact ctttgttgct gtccaccaga taatttcata
     3121 acatttcttt ttatttgttt tttatctaaa cctagttcaa acaagatact ttcatctgct
     3181 gatttattta ctaatctaat attttcaatt ggtgataaat aatctattaa attatagttt
     3241 tgaaatacta aagatatatt attttttcta tgattactat atcctttatt ttgtatatct
     3301 tcattcttaa acaatatttt tcctgtttga ggtttatcaa gtccagcaag taaggaaaga
     3361 agtgtagatt ttcctgttcc tgactttcct actatcgcat aaaacttccc aagttcaaat
     3421 ttttgattta ctcctgacaa aactttttct ttagaatttg cataactata tgctacattc
     3481 tttatttcta atatatccat tattttctcc taacttattt ttgataagat ttctttaggc
     3541 ttcttgatta acattaatga agaagcaaat acaactgata aaacaataat acttattaat
     3601 attaaataac tttgtccaag tgttgttata tttgacataa aactactatt atttattaaa
     3661 cttccaccgg aaatcattga atcctctgag ttaatgaatc catctacaat tacttttagt
     3721 aatacatttc ctaaaaataa ggaagatatt atacttggta tcgatatgaa tattaactcg
     3781 aatataaatt gcattataat ttgtatctta gatgttccaa tagataagaa tatacctatt
     3841 tcataaattc tttcccttaa ccatagaatc aagattaatg aaagaacaac catcccacct
     3901 aacataatag aataagtcat tacttttatt atatatttta ttccactcac tgactctaaa
     3961 gactcttcaa acgcattaga atctttttca acaaaatact ttgactcatc aattttaagc
     4021 tctttcaatt tgtttaaggc taagtctgta gattctgcac taccagaata cattaaaatt
     4081 ttatttgcaa tttcattatt ttctgaatta tttaatatct cttggcttgt tgaataatct
     4141 acaaacacca tattttcact aaaatcagaa gataatcctg tatatgtttc ctgtttttta
     4201 ccagaaaaga tccctataat tttaaattta tgacttttta tttttccact tttttcaata
     4261 tctagtaatt caagatcaac ttcatcacct aattttagat tgttttgttt agcaaattct
     4321 tcatgaacaa taattgaatt cttatcattt tcttctatat ttttaccttc tttaattgta
     4381 aatactccac tactaaataa aatatttctt ttagtattat ttgtagcttc gagtgaaaca
     4441 acattcttaa attcgtcaga taaatcttct ctatttattc tttgttctcc gctaactact
     4501 ttagcatctt ttagttttgc taatccatca tattgaatta ttttttcttc aatttctttt
     4561 aatttttcaa tatctttaaa ttgattaaca ttaaaatatt taccatcttt ttttgttatt
     4621 gatattgaag aattagaact ttcatataaa gccttttcta tttcattgct tgatttcatt
     4681 attgttaaac aagaatacaa gcaagaaaga actatagtta atatgataaa aattattaaa
     4741 gttctatttt tctttcttgt aatatatgct atagcatttt ttatcacttt cctaactact
     4801 cctctctatt ttcttactcc ataactaatt gcatccactg gacaagcatt tttacatcgt
     4861 ccacatctga tacactccag atgattgcaa ttctcaactg aatctttatt catttggcaa
     4921 acttttttac attttccaca attaatacac ttatccttat c
//
LOCUS       DAYYNQ010000144         4931 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000144 DAYYNQ010000000
VERSION     DAYYNQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.147"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHBJ1_002777"
     CDS             85..972
                     /locus_tag="ACHBJ1_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM9431648.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBJ1_002778"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHBJ1_002778"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM9431649.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHBJ1_002779"
     CDS             2274..2720
                     /locus_tag="ACHBJ1_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM9431650.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHBJ1_002780"
     CDS             2993..4345
                     /locus_tag="ACHBJ1_002780"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM9431651.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHBJ1_002781"
     CDS             4516..4803
                     /locus_tag="ACHBJ1_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM9431652.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DAYYNQ010000145         4899 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000145 DAYYNQ010000000
VERSION     DAYYNQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.148"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHBJ1_002782"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACHBJ1_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM9431653.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACHBJ1_002783"
     CDS             complement(2064..3062)
                     /locus_tag="ACHBJ1_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM9431654.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACHBJ1_002784"
     CDS             complement(3288..3941)
                     /locus_tag="ACHBJ1_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM9431655.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHBJ1_002785"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACHBJ1_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM9431656.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1448 a         1020 c          753 g         1678 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaa
//
LOCUS       DAYYNQ010000146         4786 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000146 DAYYNQ010000000
VERSION     DAYYNQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.150"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACHBJ1_002786"
     CDS             <1..813
                     /locus_tag="ACHBJ1_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM9431657.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACHBJ1_002787"
     CDS             786..1229
                     /locus_tag="ACHBJ1_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431658.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1598..1726
                     /locus_tag="ACHBJ1_002788"
     CDS             1598..1726
                     /locus_tag="ACHBJ1_002788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431659.1"
                     /translation="MNTFSSERIRHDKAETVYFSFVMACADFFKSLGTRANIFIKL"
     gene            2401..3369
                     /locus_tag="ACHBJ1_002789"
     CDS             2401..3369
                     /locus_tag="ACHBJ1_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9431660.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            3379..3849
                     /locus_tag="ACHBJ1_002790"
     CDS             3379..3849
                     /locus_tag="ACHBJ1_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM9431661.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            3865..4035
                     /locus_tag="ACHBJ1_002791"
     CDS             3865..4035
                     /locus_tag="ACHBJ1_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431662.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            4499..>4786
                     /locus_tag="ACHBJ1_002792"
     CDS             4499..>4786
                     /locus_tag="ACHBJ1_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFM9431663.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSIT
                     SILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTV"
BASE COUNT         1825 a          778 c          789 g         1394 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcgattat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agttttgtag agagtgacct tataggcact ctcacattac gaacgatacg
     1501 tccctcggac gtactgaaag ggtgggactt atgagcgaac taatgcaaaa tttggctagc
     1561 aaaaaagcga aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aaacagttta tttcagcttt gtcatggcgt gtgccgactt
     1681 ttttaaaagt ctaggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaagg
     1741 cgtttcaaat gtctaagtat ataaatatat aagaatagag ttaagagtgc ttaaatcgaa
     1801 ttttagaggg aattcagaca attttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acagttttag caaaggagcc ggaggcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcaactata gggagtaaga
     2041 ttcccagttt agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctgta tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacaga
     2341 cattttgtat ttcataacgt ctgtatctct tgtgcgagta agaaaggagc tattttaaat
     2401 atgtctaatg aaatcgtaaa gtatcatcat gaattaaata caattccgtt gcgaaaattt
     2461 acaccagtcg aaatgaattt gttcttctct atcgtatcaa gaatgcgtga tgtaggtgat
     2521 aaaaaagtac aatttacatt cgagcaatta aaagatttaa gcaattataa agcaactgca
     2581 aatgttcgat tcatagatga tttagaaaca acgtatgaca aattaatgga tttacgtttt
     2641 ggtagaagaa gtgctgacgg tttgcaacgt gaacgttttg ttttatttaa tcaatttaaa
     2701 atcgacggaa aagctgatat tccttttgct gaaatacaag ttcatgaaaa agccttaccc
     2761 ttacttaata atcttgaaga atgggtgcgc tattctctcc aacaattcaa tgaattagaa
     2821 agcagttatt caaaaacaat gtttcgatta ctaaaacaat acagaaccaa aggtttcgct
     2881 tatttttcaa aagaagactt tcatgaatta cttgatattc ctaaaagtta caaccaaggt
     2941 acgatcgatc aacgtgtgtt caatccaata cgacaagaac taaccgcaat ctttaaaggt
     3001 ttaacaatca aaaagaaata cggaaaaggc cgtgggaagc ctgtcattgg ctaccaattc
     3061 acatttaaac cagaaatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
     3121 gaacaaaatc gggctttaga actagctaat cgacaaactc aacacgaaaa agaaactact
     3181 tcattacaag aaagaattaa ggaacaaaag cgaaaattag aacttttaca acagttagaa
     3241 gagttagaaa aacaacaaaa aaatagagaa aacgaaacgg ataaacaaaa gttagacaac
     3301 aattccgata ttcctctaga actaagaaaa gacttacttg ataatcttca aaacttattt
     3361 aaagactaag gagtattcat gacagataaa tcaataaaag aatttgcaga agaactaggt
     3421 gtcagcaaac aagccattag aaagtatttt gataaactgc caaccaagtt gacacctaag
     3481 ttgataggtg gaaaatacgt aatatcagca aaagctcaag agtttataaa aaatagtatc
     3541 aaccaaataa atgaagttga tactgaagtt gacagtttag ttgatagtat tttaattgca
     3601 gaattaaaag aaaaaaacaa acaaattgat catttacaag gggtcataga aatacaacaa
     3661 aaattactcg accagcaaca acaactcacc ttgcaggcaa atcaacaaat cgacaaacta
     3721 caaaaacggt tacaattagt ttatacggaa gaaacttcag aaaatgaatc aactactctt
     3781 tctgaagaaa cagaatcgat agaaaaacaa acaaagaaga agcaaaaatg gtggaaacta
     3841 tggaaataac tggaggaaac attcatgaca agaaaaaagc tcatatttgg ttctatttta
     3901 ctaggaattc taggcttcag tccgatgtta ttgtcagtga tcaataaagg aaataaacat
     3961 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagagtt atttgtaaaa
     4021 gaaacggata aataataaaa aaagactgct ctaacgagca gcccaaaatg agattatcct
     4081 agtttagcaa cataaaccat ttattagatg aaaatgagat acaaacaaag cgtatcaaat
     4141 cctctcaaaa ataacaagtc tttaaaaaat gacagataaa aatgcacaaa gaattagata
     4201 tagataaccg cgaaaaatca agccttaaaa agagcttttt acctagacca atccaaaatc
     4261 acaaagtgag cctctgtatc ggttgataca agggctactt ggcatagtcg acattatgtt
     4321 aactaacgct ataaataacg attatgttcc caaaacaata ttttatgaac tataacaaaa
     4381 atttgttatc gcatattgta tattttaatt ataggtgtat accacttaaa tttaacacaa
     4441 aggcgtgatt attttgaaac ccaaaaagaa aatttatcta ggtctcctct tttccttgat
     4501 gattggaatt tttagtatag gaattacaaa tacctatgct tcagaaaatt ctaatttcaa
     4561 tatcaactcc caaccaattg aagaacaagc aattaatttt gcgaaaactg attttgaaca
     4621 ctccataact tcaattcttt taatggaaaa taacaccgaa actgatatta actatactct
     4681 tggttcgcca tttaaaatgc tcgatggaga aaatacggca aatagttcta ttttctttcc
     4741 agtattttca gacgctgata atacgattaa atatatttat accgtc
//
LOCUS       DAYYNQ010000147         4765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000147 DAYYNQ010000000
VERSION     DAYYNQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.151"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..1169)
                     /locus_tag="ACHBJ1_002793"
     CDS             complement(207..1169)
                     /locus_tag="ACHBJ1_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM9431664.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ACHBJ1_002794"
     CDS             complement(1171..2610)
                     /locus_tag="ACHBJ1_002794"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM9431665.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ACHBJ1_002795"
     CDS             2795..4741
                     /locus_tag="ACHBJ1_002795"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM9431666.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1514 a          817 c          790 g         1644 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatgg
//
LOCUS       DAYYNQ010000148         4609 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000148 DAYYNQ010000000
VERSION     DAYYNQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.152"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACHBJ1_002796"
     CDS             complement(220..972)
                     /locus_tag="ACHBJ1_002796"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM9431667.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACHBJ1_002797"
     CDS             1085..2005
                     /locus_tag="ACHBJ1_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9431668.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACHBJ1_002798"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACHBJ1_002798"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM9431669.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..>4609
                     /locus_tag="ACHBJ1_002799"
     CDS             3311..>4609
                     /locus_tag="ACHBJ1_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431670.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYN"
BASE COUNT         1616 a          747 c          895 g         1351 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagaaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcagtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaat
//
LOCUS       DAYYNQ010000149         4590 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000149 DAYYNQ010000000
VERSION     DAYYNQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.153"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHBJ1_002800"
     CDS             186..2855
                     /locus_tag="ACHBJ1_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM9431671.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3434)
                     /locus_tag="ACHBJ1_002801"
     CDS             complement(2946..3434)
                     /locus_tag="ACHBJ1_002801"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431672.1"
                     /translation="MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIY
                     DRNDLAMVLIGMPGIEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDI
                     KLEDFSDYEAITTIIKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVI
                     GI"
     gene            3680..4219
                     /locus_tag="ACHBJ1_002802"
     CDS             3680..4219
                     /locus_tag="ACHBJ1_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431673.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="ACHBJ1_002803"
     CDS             4216..4554
                     /locus_tag="ACHBJ1_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM9431674.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DAYYNQ010000150         4490 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000150 DAYYNQ010000000
VERSION     DAYYNQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.154"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      161..327
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            475..1032
                     /locus_tag="ACHBJ1_002804"
                     /pseudo
     CDS             475..1032
                     /locus_tag="ACHBJ1_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1090..3129
                     /locus_tag="ACHBJ1_002805"
     CDS             1090..3129
                     /locus_tag="ACHBJ1_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM9431675.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3238..4389)
                     /locus_tag="ACHBJ1_002806"
     CDS             complement(3238..4389)
                     /locus_tag="ACHBJ1_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM9431676.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1626 a          826 c          871 g         1167 t
ORIGIN      
        1 atcctggtgc aactgaaaga atttgcaacg ggattctttc aacttatttg ttattttttt
       61 ctaaaaaata ttaaagattg ttaaatttca ctatttcatt tcaaattcca ttgcctaatc
      121 attaggaaaa gtctatcatg aactcgtaaa tagaatatag taataacctt gttaggtgag
      181 gctcctatac aaacataggc tgttgcccag aaacgtcgaa agtcgccaat gggtaaaaca
      241 gaaactgttg gattaaggct ttttccaata gctaagaatg ttttctttac gttgtatagt
      301 gctaaaactc ggacgatgag atgatgctag gtcagaattc ttgttcgaaa aggtttctga
      361 ccttttcttt atatttttgt gactgactta tttgatgaga caaactcgct cctcacctga
      421 caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa gatcgattgg tggaggtcgc
      481 tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa actgatctct
      541 atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat aaatgcagct
      601 tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt caaatttgaa
      661 gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa tgaactgttt
      721 cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt atatataaac
      781 aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt tcgggaagac
      841 aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc tatctctcag
      901 cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa attacttggt
      961 agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga tttcctcaaa
     1021 gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
     1081 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
     1141 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
     1201 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
     1261 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
     1321 ttatctgaaa aagagatctt agatcaatac cagaatatat acggtgctgc tgatgtcaaa
     1381 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
     1441 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
     1501 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
     1561 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
     1621 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
     1681 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
     1741 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
     1801 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
     1861 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
     1921 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
     1981 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtgag
     2041 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
     2101 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac
     2161 agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
     2221 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
     2281 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
     2341 tatgctcctg gatctacgtt taaaataatt acagcagcaa tcggtctcga caacggcact
     2401 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
     2461 ggatctttta aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
     2521 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
     2581 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
     2641 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
     2701 ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt
     2761 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
     2821 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
     2881 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
     2941 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
     3001 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
     3061 gatgatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
     3121 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
     3181 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
     3241 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
     3301 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
     3361 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
     3421 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
     3481 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
     3541 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
     3601 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
     3661 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
     3721 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
     3781 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
     3841 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
     3901 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
     3961 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
     4021 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
     4081 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
     4141 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
     4201 taagaacaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
     4261 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
     4321 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
     4381 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
     4441 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag
//
LOCUS       DAYYNQ010000151         4461 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000151 DAYYNQ010000000
VERSION     DAYYNQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.155"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            574..1008
                     /gene="bacA"
                     /locus_tag="ACHBJ1_002807"
     CDS             574..1008
                     /gene="bacA"
                     /locus_tag="ACHBJ1_002807"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFM9431677.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="ACHBJ1_002808"
     CDS             2298..3221
                     /locus_tag="ACHBJ1_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9431678.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="ACHBJ1_002809"
     CDS             3237..3776
                     /locus_tag="ACHBJ1_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM9431679.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1663 a          666 c          760 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc gtctctatcc attggtagag
      421 gggctacttg gcatagtcga cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAYYNQ010000152         4433 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000152 DAYYNQ010000000
VERSION     DAYYNQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.156"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHBJ1_002810"
     CDS             complement(543..2048)
                     /locus_tag="ACHBJ1_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM9431680.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHBJ1_002811"
     CDS             complement(2045..2731)
                     /locus_tag="ACHBJ1_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9431681.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHBJ1_002812"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHBJ1_002812"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM9431682.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAYYNQ010000153         4335 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000153 DAYYNQ010000000
VERSION     DAYYNQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.157"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHBJ1_002813"
     CDS             127..579
                     /locus_tag="ACHBJ1_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431683.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHBJ1_002814"
     CDS             593..1282
                     /locus_tag="ACHBJ1_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431684.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHBJ1_002815"
     CDS             1407..3758
                     /locus_tag="ACHBJ1_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM9431685.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACHBJ1_002816"
     CDS             3838..4233
                     /locus_tag="ACHBJ1_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431686.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DAYYNQ010000154         4325 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000154 DAYYNQ010000000
VERSION     DAYYNQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.158"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..920
                     /locus_tag="ACHBJ1_002817"
     CDS             <1..920
                     /locus_tag="ACHBJ1_002817"
                     /inference="COORDINATES: protein motif:HMM:NF016640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase domain-containing protein"
                     /protein_id="HFM9431687.1"
                     /translation="SLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLNQMKKRDN
                     LLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCITGNYDSIK
                     EPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPNELFTDYD
                     FTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKGNKESIIE
                     QISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQLAFPYVL
                     WKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1172..2182
                     /locus_tag="ACHBJ1_002818"
     CDS             1172..2182
                     /locus_tag="ACHBJ1_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HFM9431688.1"
                     /translation="MKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQLKE
                     DRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAYID
                     SNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKKSN
                     ELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKMERD
                     FTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFVET
                     IEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTVYY
                     LS"
     gene            2550..3974
                     /locus_tag="ACHBJ1_002819"
     CDS             2550..3974
                     /locus_tag="ACHBJ1_002819"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM9431689.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4060..>4325
                     /locus_tag="ACHBJ1_002820"
     CDS             4060..>4325
                     /locus_tag="ACHBJ1_002820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431690.1"
                     /translation="MIFGKKHSLVEVPEFGSEPYLIEIGDNFRCSFGVKFITHDGGVW
                     VLRKMSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVII"
BASE COUNT         1479 a          561 c          787 g         1498 t
ORIGIN      
        1 atagtttagc tatcaatgaa ttggaaaaaa gaaatcttga aatacatgaa ttaaatcaaa
       61 aaaataattc tattttaggc atgaaaatac gaaataaaat taatcaactt aaccaaatga
      121 aaaaacgaga taatctgttg ccagcaaaat atacccttaa atcgatgacc ggtaaggggc
      181 ctattaaaga ctattcaaac agagagaatc gcagtgatga actaagaaaa aaagagttaa
      241 ctaatcaagg aacacctaaa aggattgctg tgtatagttg tatcactggt aattatgatt
      301 ctattaaaga accggatttt tctcatccat tggtagatta ctatttgtat acagataatg
      361 tgattacaag tgagcattgg aatattagag agatacctaa atatatatcg gaattaaaaa
      421 ataatatttt gatcaataga tatattaaat ttcatcctaa tgagctattt actgactacg
      481 attttacttt gtatgttgat ggtaatgtaa caataacttc tgatattacc ggattactga
      541 acagttcttc caaacttggg ttaagtatgc ataaacatag tgaaagaaaa tctttatatt
      601 ctgaggcaga tgcattaatt gcagttggca aaggtaataa ggaaagtatt atagagcaaa
      661 ttagtaagta cagagaagaa ggatttcctg ataattttgg attggctgaa gccacggtta
      721 ttttagttga tttaaataat tcaatcgctg agaatcttct agatttgtgg tggagagagt
      781 atttgaactc agaaagtggg cgagatcagc tcgcatttcc ttacgtgttg tggaagtccg
      841 gacatgcaat gaaggaagtg actaccttag gcaataatgt atataaaagc gctaaattaa
      901 gaatcataag tcattattag ccctaaatga tttctaaact attctttcaa atcctagtaa
      961 atatttggag atagaagatt tcctttttaa agtcataaaa aattaagtta tattttttga
     1021 gtgaatggag aagacgcata gtatcataaa cttcgttata tcttaaatta tggggtatgc
     1081 ctaaataata aatgtaatac gagtataaag aagaaagcaa ttttgggttt aaccctattg
     1141 gttcctttca gaaaaaggtg gatgaataaa aatgaaaata ttaataacag gtggagcagg
     1201 atttattggt tttcatttgt ctagaaaatt actcaaggaa actgaaaatc aaattgtagc
     1261 gattgataat ctcaatgatt attatgatgt ccaattaaaa gaagatcgat taacgatact
     1321 gtacgattat aataatttta aatttatcag aatggatcta ttagagaaag aaaaattaga
     1381 ttctctattt gaaagtgaag gttttgacat agtaattcat ttagctgctc aagcaggtgt
     1441 tagatattct attgaaaacc cttatgctta tattgattct aatattatcg gttttcttaa
     1501 tattcttgaa gcctgccgaa acttccctgt aaagcatcta ttatatgcat catctagttc
     1561 agtatatggt gggaacacga aagtaccgtt ttcaacagaa gattcggtgg atcatccagt
     1621 tagcctttat gcggctacca aaaaatcgaa tgaattaatg gctcatactt atagccacct
     1681 ttataaaata ccaacaactg ggctgagatt tttcactgtc tatggtccat ttgggcgacc
     1741 agatatggcc tatttctctt ttaccaagaa tattttatct ggaaaagaaa ttaagatttt
     1801 taaccatgga aagatggaaa gagatttcac ttacatcgat gatatcgtag agggaatcac
     1861 tagattaatc aataaagtac ctgaacccaa ctccgactgg acagacatcg atgatttgag
     1921 tagtgaaagt tgggcaccat ataagatata caatattgga aataataagc cggttgcatt
     1981 gttggagttt gttgaaacaa ttgaaaagta tttaggtgtt aaagcgaaca agattttttt
     2041 ggacatgcag ccgggtgatg tcgttagaac ttatgccgat attgaagatt tagaggaaaa
     2101 aatcaacttt aaaccgaata taaccttgga tgagggcatt aaaaaattcg ttgaatggta
     2161 tacagtgtat tatttgagtt aattttgaaa ggaatattga agatgggtaa ttcatacgat
     2221 ttttttagct ttgttaggta ttttaggaaa ttctactttc aaacgtgacg aactttctcg
     2281 ccgaatagat attttctata actaaaagaa tttctataaa acaaaattgt gtatcaagat
     2341 gtgtgaactg gattctgact gccaatattg attgatgaga ttgaagaaat atatagttga
     2401 tcatggatat ctttatttga aaagatctta gctattcaca tattcaaaaa aatttttact
     2461 acgaggactc acacgataca gaggaattta ggtagttcta attaaaccag aaagtcatct
     2521 atagatgtga gttgagggag gtcatacggt tggatagtag cagtaataaa tcacattcaa
     2581 tagctaaaaa agcttttatt tacgcaatag gaacattatc atcaaaaatg atatctgtca
     2641 ttataatccc tatctatgca gtatatattt ctgttgccga tttaggaagc tttgattatc
     2701 aaaatacaat tgctcaattt ctttctccag ttatttttgg agcaatatgg gaagcaattt
     2761 tacgcatggg attaggcaaa gaaaaaaata ttcagaaaaa agtagtgacg aacagtattt
     2821 gttttgttat agcaaataca gtgatattag ccattttttt accaataata tatagaatga
     2881 ttttcccgaa tttagagtat tatcaattgt ttgtttttca attaattttc actcctatcc
     2941 taagtgtttt tcaatatatt gtaagagcac taggtgaatc aaagattttt gtgatgagtg
     3001 gtataatcgc tgcgggtatc aacctgttac tccttactat ttttgttgtt acaatgggta
     3061 tggggatact tggtctctta ctctctatca taatttctaa tgttataaat ttgatttttt
     3121 tgattttcag ggggcagatc tttcaatatt tcgcaacgag ttcttttgat tttttatttt
     3181 taaagtccat gctgaaatac tctatcccac tcactttgaa tctcactttt ggatggctaa
     3241 tagttggttt ttcaagattt tatattggaa cgtacatcgg agtaacagaa aatggtattt
     3301 tttcttttgc gatgaaattt tcatctataa ttttaactct tacaagtatt gttagtatgt
     3361 ctatgataga ggatgcggtt atcacgtctg agaatgatga tttcatcgaa agattcgttg
     3421 agaaaaatac ctatattttt aaaatgtaca ttaggttagc gactctttct attcctatca
     3481 tttgctgtgc atactctttt ataaccaacc ctgattataa aaaatcatta gttctagttc
     3541 caatattagt tattatggca tgtttcagtg ggatgacaac aaatatcgga aatatatttg
     3601 cagtatacaa taaaacaaat cagttattaa tgatttcagt agtgtcaggt ctattgagtg
     3661 ttgttggcac ctatttatta ggctattctt ttggagtaat tggtgtctca tttgctactt
     3721 tgctcggtac tttagtatta tttggactta gatattattt tggaaggaaa attgcttatt
     3781 atgcatttga ttggaagcga atatcgctac ttttagttct ctattccgca atcgcttttg
     3841 tatgcattca tagaggcttc tattttaatc ttttcttaac gattattttt agtgctattt
     3901 tcgtttttac gtataaacaa ttaattataa cgtatctaaa aaaatttttc agaataaaaa
     3961 tggctaaaag gtgagggtat acgaagtaag aatagaatct ttcgaagaat aaagagaaaa
     4021 ctatttggaa aaaatctgcg agaatgtatt aatgaaggtt tgatattcgg aaaaaaacat
     4081 agtttagtag aggttcctga atttgggagc gagccatatt taattgaaat aggggataat
     4141 ttcagatgtt catttggtgt taaatttata acacacgatg gtggagtctg ggtcttaagg
     4201 aagatgagtt ctgtgagcga gtcaacttca aaaataggta ggatcaaatt gggaaataat
     4261 atttttattg gtatttactc tattgttttc ccaggagttt caattggcga taatgtaatt
     4321 attag
//
LOCUS       DAYYNQ010000155         4263 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000155 DAYYNQ010000000
VERSION     DAYYNQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.159"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..768
                     /locus_tag="ACHBJ1_002821"
     CDS             67..768
                     /locus_tag="ACHBJ1_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM9431691.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1340..2356)
                     /gene="tsaD"
                     /locus_tag="ACHBJ1_002822"
     CDS             complement(1340..2356)
                     /gene="tsaD"
                     /locus_tag="ACHBJ1_002822"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM9431692.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2353..2817)
                     /gene="rimI"
                     /locus_tag="ACHBJ1_002823"
     CDS             complement(2353..2817)
                     /gene="rimI"
                     /locus_tag="ACHBJ1_002823"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9431693.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2824..3366)
                     /gene="rimI"
                     /locus_tag="ACHBJ1_002824"
     CDS             complement(2824..3366)
                     /gene="rimI"
                     /locus_tag="ACHBJ1_002824"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM9431694.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3350..4072)
                     /gene="tsaB"
                     /locus_tag="ACHBJ1_002825"
     CDS             complement(3350..4072)
                     /gene="tsaB"
                     /locus_tag="ACHBJ1_002825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM9431695.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1260 a          914 c          735 g         1354 t
ORIGIN      
        1 aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata aggagaggac
       61 tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata tcaacctgat
      121 tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt tcttcgttct
      181 tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt gtatatcttg
      241 accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta ctactatgac
      301 tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga gacctacact
      361 tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc ctactatgaa
      421 aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat ttctaaagta
      481 gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt tgaccgggta
      541 aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga tggtcatatc
      601 accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac gacacgtgaa
      661 actgctggac aagtaatcaa acaactacgt gattccggac tgttagatta tgacaaaaag
      721 cgttttactt tctgtaagac agctactact ccagctatga gagaataacg cgaacaaaaa
      781 taagcagtga ccctgatatt tatcagggtc actgcttatt ttcctcccta aaacttctga
      841 aagatccggc agttttagat cactgggcaa gactcaaaga ttttctatgt gtgtttcagt
      901 tatgtaatat tcactgtttg caaaaaatac tgatagatta actagaggaa gcgtcttgtc
      961 ctcatccgac ctagattatt cgatattcct cgacaataaa cgattaatca gtgagggcaa
     1021 tcagaggaac ggataatgat gaataagctg taaaaaatag ggctttgaca aaagcgtgtc
     1081 acagccctta tttcgattaa acggtgtgta cctgtcaact cttcggtcac aagtcataat
     1141 gttcataaac atgaggagct gttttttgaa attgttcctt gtgaatcaga gttaaacgac
     1201 aggcttacga actcttgatt aaacggcgtt caaaagcttg catacgcggt aataagaaca
     1261 gccaggtcaa aaacatgaga agccgttttt cctgggctgt tcttattact tgatgcaggg
     1321 cagatttttc acaacctctt tattcagata aaaccaagct tggatcagca ttcaactgat
     1381 agctagcaaa atttcttttt tgcatttcca catgtgctgc tgctccaatc attgcagcat
     1441 tatctccaca aagtcgaaga gggggaatca ctagttctac ctcagggagt ttagttgcca
     1501 aagcatcctg caatccttca cgcaaccctt gattggccgc tacacctcca gctacaatca
     1561 gttgcttgac tggataattt tgacaagctc gaagtgtttt agtaatcaat acatcgatca
     1621 cacttgcttg aaagctagct gctaaatcat ttttgtctag atcttctcca cgctgttgtg
     1681 cattatgcac caaattgata aatgcacttt ttaatccgct aaaactgaaa tcataattat
     1741 cttcatggat catcgctcgt ggaaaatggt aattatcttt tccctgatga gctagctgat
     1801 cgatttcttt tccgcttggg taagaaagac ctaatactct tccaactttg tcataagctt
     1861 ctcctgctgc gtcatctctt gtctccccaa tgatttcata tgatccatct tcttgcatat
     1921 agactaattc tgtatgtcct ccgctaacaa gaagtgccat caatggaaac tggaatggtt
     1981 taaccagacg ggctgcatag atatgccctg ccatatggtt tacaggaata agcggcaatt
     2041 ggtgtgccca agcaaaagcc ttagctgcag aaataccaat cagcaacgag ccgacaagtc
     2101 ctggtccata agtgacagcg actgccgata aatcttctgc cgacacacca gcttctacca
     2161 gtgcatcttc caagcataag gtgatttgtt ctacgtgatg gcggctagct acttcaggaa
     2221 ctacgccacc aaaacgttta tgactgttta tttgagaagc aacgatgtta gataagatct
     2281 ctgttccatt tctaacgacc gctacactcg tttcatcaca acttgattca atcgccagaa
     2341 tcaattcttc attcatttca tcgtctccgt ttttaacttt ctgctcataa tgatcgcatc
     2401 ttccgtcgga ttgcgataat atgatttccg tttacctaat atacgaaact tttctgattc
     2461 ataaagtttt cttgctgctt cattagatag acgtacttct aaaaaaacat gcagcgaatc
     2521 ttttatttgt tccgtaatca gtaattgtaa caaatgccgt gcatgccctt ttccttgttc
     2581 tgaaactgca acagcaatat ttgtgatctc gacttcatct attactttcg agtatccaat
     2641 aaagccacag atctggttat cttcaactaa aagcagataa tcagagtgag gctgttggat
     2701 gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt ggataagcat tttcgctaat
     2761 catccatacg cattctgcca agtacgcttc atcaaaatct ttttttctgc aaatcaacta
     2821 atctcactca ttttctaaaa gttttatcat atccaacgca tcttcttttg tttcagtgta
     2881 atatcttttt ttgatcgttg ttgcttgaaa acctaacttc cgataaagtc tttgtgcatc
     2941 ttgattactc atacgcactt ctaatgacat cgtctcacag cgattcatta tagcaaagtt
     3001 ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc tgatatgccg gtcgaacggc
     3061 aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa ccgataaaac caatcattct
     3121 tccattatca aagataccta gatatagatg aggaagcggt gacttcaatt ctgatataaa
     3181 ggcacttttt gtccatggca attcgccatt atagacttct cgttcgacat ccagcagctc
     3241 tttgatatct tctgtagtca attctcttag tacgtaacga cgatcgattt gttcgattgt
     3301 tttttctgta taaatcattt tatcttgaaa aaagcttctg gcatacgctc taaatttttt
     3361 caacataact ttcgtctcca ggtgtgtgcg tctccaacca tttttcttct gcttctaccc
     3421 gttttaaata gcgaggaaga aaagcttgtt tctctgcaac tggttcagct aaagcaccta
     3481 attgagccac agttacacca cttggaatat cccatgctgg aactgtatta atcatcgcac
     3541 taggcaatat ttcctttatt tcatagaaga atttgtgaca atcactcccg acgaaataaa
     3601 tagcttcatt cagcttttgt agttgtgtga acaaatcaga agcagaaata tgtacatctt
     3661 caagtacaat ttctaatcta ttttctttcc agcggtatgc cccagcgtat acattttttc
     3721 ttcgagcatc aaacatgggc acaataattt tatccacacc gatgcaatta gccgcgatgg
     3781 ttttcagact agatactcct actagctctt tatctaatgt ataagcaatc gtttttgctg
     3841 tcgttacacc tatacgaagc cctgtataag aaccgggacc atcagaaacc gcgattcggt
     3901 caatgtcttt tggacttatt tgaagatctg caaacaattg gtcgattgca ggcatcagtg
     3961 ttacactgtg gttgcgtttg atatttgttt gtatttgtcc aagaatttgt tcgtcttcta
     4021 ccacaccgat cgccaaagtt tggtttgcag tatctattcc taatgtaatc atatatttga
     4081 tcctttctgc tatgacattc tcgccttcca ttgtagcata cttttttatt ctgaggatga
     4141 aaataccaaa ggcaaaacta gtacaatgat acatgattat ctgctggcgg gcaaattttg
     4201 ccccttcctg aaacacaaaa aagctgcggt atcacttcat ttccccacag cttttccatc
     4261 ttt
//
LOCUS       DAYYNQ010000156         4154 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000156 DAYYNQ010000000
VERSION     DAYYNQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.160"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(352..1065)
                     /locus_tag="ACHBJ1_002826"
     CDS             complement(352..1065)
                     /locus_tag="ACHBJ1_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM9431696.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(1319..2020)
                     /gene="nagB"
                     /locus_tag="ACHBJ1_002827"
     CDS             complement(1319..2020)
                     /gene="nagB"
                     /locus_tag="ACHBJ1_002827"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM9431697.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            2254..3786
                     /locus_tag="ACHBJ1_002828"
     CDS             2254..3786
                     /locus_tag="ACHBJ1_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM9431698.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(3977..>4154)
                     /locus_tag="ACHBJ1_002829"
     CDS             complement(3977..>4154)
                     /locus_tag="ACHBJ1_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9431699.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         1334 a          855 c          711 g         1254 t
ORIGIN      
        1 ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc
       61 ctttgatact tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt
      121 aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac
      181 ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa
      241 aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt
      301 ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc
      361 aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc
      421 ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc
      481 atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata
      541 atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta actttttagt
      601 cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact
      661 gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca
      721 taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc
      781 gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat
      841 cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac
      901 ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc
      961 tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc
     1021 taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag
     1081 tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa
     1141 aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca
     1201 gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct
     1261 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt
     1321 acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata
     1381 cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt
     1441 tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca
     1501 ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga
     1561 ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat
     1621 tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat
     1681 cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta
     1741 caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc
     1801 tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac
     1861 tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg
     1921 ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt
     1981 ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt
     2041 ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa
     2101 ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg
     2161 ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta
     2221 tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt
     2281 acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc
     2341 gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc
     2401 actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt
     2461 tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact
     2521 gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc
     2581 gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa
     2641 ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat
     2701 gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt
     2761 ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac
     2821 ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat
     2881 atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc
     2941 gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa
     3001 caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc
     3061 gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa
     3121 aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt
     3181 gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc
     3241 ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt
     3301 gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag
     3361 attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca
     3421 caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt
     3481 ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa
     3541 ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca
     3601 cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat
     3661 caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc
     3721 gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca
     3781 aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac
     3841 tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg
     3901 caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt
     3961 cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg
     4021 tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc
     4081 catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca
     4141 taaacataaa taca
//
LOCUS       DAYYNQ010000157         4070 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000157 DAYYNQ010000000
VERSION     DAYYNQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.161"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHBJ1_002830"
     CDS             85..1434
                     /locus_tag="ACHBJ1_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9431700.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHBJ1_002831"
     CDS             complement(1562..2152)
                     /locus_tag="ACHBJ1_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM9431701.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBJ1_002832"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHBJ1_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM9431702.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1175 a          745 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaac
//
LOCUS       DAYYNQ010000158         4065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000158 DAYYNQ010000000
VERSION     DAYYNQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.163"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACHBJ1_002833"
     CDS             complement(<1..1155)
                     /locus_tag="ACHBJ1_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9431703.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACHBJ1_002834"
     CDS             complement(1158..3407)
                     /locus_tag="ACHBJ1_002834"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM9431704.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACHBJ1_002835"
     CDS             complement(3504..4007)
                     /locus_tag="ACHBJ1_002835"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431705.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1310 a          743 c          580 g         1432 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attac
//
LOCUS       DAYYNQ010000159         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000159 DAYYNQ010000000
VERSION     DAYYNQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.164"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHBJ1_002836"
     CDS             complement(717..3866)
                     /locus_tag="ACHBJ1_002836"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFM9431706.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          636 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYNQ010000160         3874 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000160 DAYYNQ010000000
VERSION     DAYYNQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.165"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            283..1008
                     /locus_tag="ACHBJ1_002837"
     CDS             283..1008
                     /locus_tag="ACHBJ1_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HFM9431707.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            998..1672
                     /locus_tag="ACHBJ1_002838"
     CDS             998..1672
                     /locus_tag="ACHBJ1_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFM9431708.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(1960..2244)
                     /locus_tag="ACHBJ1_002839"
     CDS             complement(1960..2244)
                     /locus_tag="ACHBJ1_002839"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HFM9431709.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(2538..3845)
                     /locus_tag="ACHBJ1_002840"
     CDS             complement(2538..3845)
                     /locus_tag="ACHBJ1_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM9431710.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
BASE COUNT         1244 a          687 c          697 g         1246 t
ORIGIN      
        1 atgaatgcga aaacgatatt tttttttatt ggaaaatatc ggtcaagcaa taaaccagtt
       61 gcataataaa atgttaacgg caacaaaatc tgtggaagga atgaaccgga tgatttgatg
      121 gaaaaaacaa gatagacaag aagtatacat agtaaaacgt ggaacaagta ggccagaatg
      181 aaaaatcagt ttttatccac tcgcttttct atacattcac tgtcacaaat ataatcgaaa
      241 gtaattctat tttgagataa agtattttat aggagatagt atatgaacaa aaaaagattt
      301 aaacgtattg aaagaaaatt tgcattaact tatgaccaag ctaagtattt gaaaacacta
      361 tttccaaaac aggtagttcg ggataaatat agtgatcatt ttatttcttc cttgtatttt
      421 gatacaaacg atcatcactt tttaaaagaa atcactacag aaaagtccta tcgagaaaaa
      481 tttagaatca gaaaatatgg agatcctcag gacatatcgc ctatctactt agagataaaa
      541 agaaagtttc aaggaatgac agataaacgt agaataaaat tagcgcatca tgagctcgat
      601 gatatgttgg aaaatatcga attggcagaa accacattca gtaatttaga agaagagtat
      661 atttttaatg aaatacagtg gttgttcaga aggttggcat tggagcccaa agtatatatc
      721 tattatttaa gagaagcatt tgtaaataga gattcagaag atttgcgaat cacttttgat
      781 caaaatatcc attaccgtct aatgaactta acgacctggg aacactgtac agaagacaat
      841 ctggtagcgc cagaaatcga tgtgctgatg gaagtaaaaa cgacaggaga aatacctgaa
      901 tggttacagg taggattgga taggatgggt aaaaaagaga taaaattttc taaatataaa
      961 caagtctata aaagatatat ccaagaggac ccgctctatg ttcaatagtg tcttaaaaaa
     1021 tgagccttct caactcacac tagatgattt ctttttatgt atggtggtgt cgattttgct
     1081 aggcattctg attgcttaca cccatatgta tcaaaacaac tgtacaaaaa acttcatgtt
     1141 tgccttgatt gggttaccta ctttagtaca taccgtcatt ttgttgatca acggtaactt
     1201 aggcacgggg atcgctgttc taggcgcatt taacttagtg cgatttcggt ctgctcaagg
     1261 gaattcaaga gatattttca gtattttttg ggcaacgtcg attggtttag ccacagggac
     1321 gggatatatt gtctatgcac tattcgtgtc tagtatagtt gctgctatcc aagtcatcat
     1381 cttcacgctt ccaatcaaaa ataaacatac agtttttaat actgaaaaaa cgatcaagct
     1441 aaaagtttct aaatccttgt ttctttcgag tattattgaa gaaaaaattg cagaatatac
     1501 agaaaactgc aatttaaaaa aagtaaaaat caataaagaa acttgttcct tacactatat
     1561 agtaaaagta aaaacgagta tgcttgaaaa agaattggtt cgaacgatca tggatcatcc
     1621 agaagtcatt tcgatcactg taggtatgga taaatcgaat aaattattgt gataaccaag
     1681 aaagtactta acaatgaagt aagaccccag ttccaaagtt tagcggggac acttcaataa
     1741 agtactttct tttttgtctt cagcaactac tggtaaaact gagagcaagc tttcgtgaga
     1801 gtacagcgat aaagatcaac ctcccttttt atctcgagca atttccttgc aaggctgttt
     1861 cgttgatctt aaacgagtta gcaacagtca aagatgcgat ggccataaaa aaattgcatt
     1921 aagttggctg gtatctgata gctgggaatc taaggaagac tatccattct gtttcttaaa
     1981 ttccgctagt tgatagatat cagccaattg ttccagatga tcaacatcaa agataaccga
     2041 aagatgtttc cttttattcc catttgcttg aagagttttt atcttctctt tggttccttc
     2101 actcaatgag agtgtgacta atttctccct cgttttttct gggaaatgaa aggatacttt
     2161 gaaaaatccg tcccaaatag aaagccagaa gatcggtttt actttctgag agtgggacaa
     2221 aagtaagaaa cacttttgtc tcattcttta aaaacgaata aacggtggga gcagaagcaa
     2281 ctccttcgga aataagccaa aattcacaaa aatttgaaaa gcaattttcg tgaatttctt
     2341 cttatttctc ggagttaaac acttctgtcc caacctcttg tattcacctt ttgatagcca
     2401 agcttttcca tcattataaa accgcaaatt tcaaaaaaaa acgaacaata caaaaagcgt
     2461 attgctcgtt tttctcttca gtttctcaca cctaaacgac ttttgtctcg acctcttttt
     2521 ggtgactatt catgatgtta aaataaatta ctgaaaaaat ctgtgacatg gcgccaacca
     2581 atgacaaaaa tattagcttt ttcaacagta gaagtggtaa tgatgtctgt cgaaggctct
     2641 tggtttgctt ccaaatagcc taatttgtca tcagctaatt ggatcgttgc tgagccaact
     2701 gtagtgcctt tgttgatagg agcggtcact tggtcatccg ttactttctt tttatcaatt
     2761 gttggagtaa tcgttaattt tccagtatcc attccatttt cgacccagac ttttacaggg
     2821 ctttttagcg caacattgac ggaggtttct tttccatctt tcactttgat gtcttttagg
     2881 cttgggatgc ttgcatttgc ttttccaagt tctttttctg accagttgtc ataacaataa
     2941 tccatcaatc gtgatgtttc attgaatcgg gcacttggat tttcttcatg gttatctgca
     3001 tttaaaacga ctgtgatgat tcgtcggcca tctttgttga tcgttccgac aaagcatgca
     3061 cccgcaaaat cagttgtgcc agtttttaat ccatcgacac cttcgtgata gtttacaaaa
     3121 ccaggtagca tccagttcca attgaccatt tcaaccggtg actgtgtgtt ttcgccgaat
     3181 gtttgatttg tggtactcgt gatttttaag acttcaggat aatctaagat gaggtgacga
     3241 gcaacaatag cgacatcttt cgcagacagt tgattttcat ctgtttcacc agtattctca
     3301 ggtcgattct cacctaggta agaattgttt aaacctgatg cattgacgat cgttgcatct
     3361 ttgatacccc aatcttttag ttgtttcttc attagctcaa gaaatttact ttcactgcct
     3421 gcaacttttt cagctaaagc aaccatggaa gcattggctg attggatgaa agctgagtca
     3481 acaagttctt tcactgtata atcattttct ttatgcaaag gaacattcga tagatccggc
     3541 acgacactta atttttctgc ataatccgaa atggctacct tatcttccca agataattta
     3601 ccctctttga tttgttcaag tacgatatat agaccaatga tctttgtgat ggaagcaatt
     3661 cccatcgttt tttcgccatc ctgatcataa aggatttttc cactttctgc atctacggca
     3721 aaggcagctt tggcatttat tttaaatgcg tcgtctgctt gaacagttgc gctaggcaag
     3781 aaagaagtga tacataagat aagacttgct aatagtacaa taaaacgggt attttttttg
     3841 aacatctttg ttctcctcat ttttaaattg attc
//
LOCUS       DAYYNQ010000161         3867 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000161 DAYYNQ010000000
VERSION     DAYYNQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.166"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..187
                     /locus_tag="ACHBJ1_002841"
     CDS             <1..187
                     /locus_tag="ACHBJ1_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM9431711.1"
                     /translation="KQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWE
                     EIKASEVPKNAAEKIDN"
     gene            complement(428..1432)
                     /locus_tag="ACHBJ1_002842"
     CDS             complement(428..1432)
                     /locus_tag="ACHBJ1_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM9431712.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            1598..2449
                     /locus_tag="ACHBJ1_002843"
     CDS             1598..2449
                     /locus_tag="ACHBJ1_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM9431713.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2466..2711
                     /locus_tag="ACHBJ1_002844"
     CDS             2466..2711
                     /locus_tag="ACHBJ1_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431714.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            2898..3602
                     /locus_tag="ACHBJ1_002845"
     CDS             2898..3602
                     /locus_tag="ACHBJ1_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431715.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1273 a          735 c          676 g         1183 t
ORIGIN      
        1 caagcaagcg gcttataata aagatggtga aaaaaaacag gtgacgcttc gcgaagaaag
       61 agaacatcca ttaaagatga atgcgtatct taaactcaaa gtcaatcctc gtaaaggtgt
      121 attaagctgg gaagaaataa aggcttctga agtgcctaaa aatgcggcag aaaaaatcga
      181 taattagtaa atggtcttaa aattttatta gataaaaaga ggctgggaca gaagcgttta
      241 actccaagaa ataagaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
      301 agacaaaagt gttctttact tttgtctcac gctctttttt tgttatattc gtcttttccg
      361 atccttctct tgacacaatt catttaactg atcactggta cttgaacacc taatattcta
      421 gtcattttta taaacgattc aatacttcat ccacattttt gatcatcacg gatatcgtac
      481 catccggatt attcacaaat tcaatcaaat ctgggttgcg atatacatct actggtacga
      541 tcagttcgat cccgtttgat agcttgaatt tttgttttcc aaattttttc tcagaaattt
      601 cttgtacttc ttttacagga ggtgcttctg ggacaaatcc ttgctcttgt acttcttctt
      661 taaaagccat ttttgccgtg atgttttcct tgaagacttt ttccgcaatc atctcatgat
      721 ccaaccgacc tttttcttct atcgtatcat agacagcttc tttcaaatca gccatgatat
      781 caaattcttc tgtctcaaat ttttttccaa gatgcttggc tactttttgg atcactttta
      841 cattttcttc taaagaaggt gcaggaacac tttcgatcac tcttccagaa aaataaagct
      901 cttttttgcc agaaaactca tatttttttt caattaattc gtaggtccaa tcagtcaaat
      961 tcacagcgaa tgcttcatct gctttttgtg ttttcgcccc tagaattgct cgattcaaaa
     1021 tcaagcggtt gtcgatccct gcttcgtctg cttcaacaaa gtgagtgtaa gcctcacgat
     1081 aattgacttt caataaagat aagtacatca ctgtatctaa ttcatacaag acaacaaata
     1141 catctgcact aggtgcttct tcactttctt ggtagatatc gaaccaccgc tgaacgattg
     1201 cttcacttgc ttccacaaaa ttttcttgag cgattttaag tgattcaacg atcatgcttt
     1261 cttctgctag ttgtccagtt tttgtttgag cagaagagat tttttggatc ttcttttgaa
     1321 gaaaatcacg aatatattct tttgtcagat ccagctcttt ttgtgaaaag actggagaac
     1381 cagtttctct gtcaataata tgcagaattg ctttttttaa atagatatcc attttctcat
     1441 tcccttctgt cttagtttac ggaaaaaaaa tcaaaaatac cagaatcaaa atcattttct
     1501 tttaaacaaa aatttcaaga gtagaaacaa aatggattct ttttttcgaa aaatgcgcta
     1561 cacttatagt tgaacgaata aaaaaggagc tgaaagtatg aattcattaa caagcactta
     1621 tcgtctatca aatggttacg aaatcccggt agtaggattc ggcacatggc aaacacctga
     1681 tggggatgtc gctgtttcct ctgttaaaga agcgctggct gcaggttatc gccatatcga
     1741 tactgcccaa ggatacaaga atgaagaaag cgtcggccaa gcgattaagg aaagtggaat
     1801 tcctagagaa gagatattct tgacaacaaa attatggaat gcaaaccaca gctatgaact
     1861 tgtgatgtct tcttttgaag aatcattgaa gaagctccaa acagattatt tagatctgtt
     1921 tttgatccac tggccaaacc ctgttgcttt tcgtgataat tgggaacaag caaatgcaga
     1981 tacatggcga gcatttgaag aactgtatga agcaggtaaa atcaaagcaa tcggtgtgag
     2041 caatttctta ccccaccatc tagatacact agcaaaaaca gcaaaaatta tgccgatggt
     2101 caatcaagtt ttccttgcac caggcgaatt acagacaact gtagttgagt atgcaaaaaa
     2161 acatgatatg atcctcgaag catatagtcc attaggcact ggtaaaatct tcgatgtgcc
     2221 tgaaatgaaa caaatcgctg aagcacatga caagacgatt gcacaagttg ctttgcgttg
     2281 gtcgctgcaa cacgaattcc ttcctcttcc taagtcagtg acaccaagca gaatcaaaga
     2341 aaatactgaa ttatttgatt ttgaattaac agaagaagaa atgaaacaaa tcgatcagtt
     2401 agatggtgta gtcggcaaag caaaagaccc agatactacc caattttaag tcgaaaggtg
     2461 attttatgga tccaatcgaa cgcttgaatt ctctctcgga agaagttacc cagacttttc
     2521 attccgattt tgtctttttg atcgatgcag agaaaatcca acattttcct gcaagaaact
     2581 ggacacatga tcaaatcatc gaagaactga aaaaacggtt cgatcattcg ctaatggtca
     2641 aaccctggca cgaacatgaa gtcatttatt cacctgaact acctgttttt gccttgatac
     2701 cgaaaaaata gaaagaatgg aacggattat gtatatatac ataatccgtt ccatttttta
     2761 tttttctttc aaaaaacggg aaaatacgca aatttttctg ttgtaatttg cgtatacgca
     2821 agtttttttg cattactcct tttcatttgg ctgttttttt cttataatcg aaagggtatg
     2881 agcgaaggag agaacatatg aagaaaaaac agaaacaaaa attattaaat caattccgtc
     2941 cttcattgga tggtgtaaga ggacaattat tccgttctct agaagaagag agcttgcgac
     3001 gttatggttt gccgttacaa gtcatgcttg atcccaaaaa ccgtcaaatc ttaacgatcg
     3061 gattagctgg gaaaacaaat gaagatacac ggatcaacgt gccaatcgat gacaacttca
     3121 atactgtatt aaagagaatt cgttcaggag aaaacgggat tttcgaacga ttccgtgaca
     3181 atcttcttat tgaaatcgtg tcttactgga atgatcaacg attgaaaaac gatgaaccaa
     3241 ctgcgcaagc tgttgcgaca ccagttacag aagaaacagt cagtgtgcca gaagaaaaaa
     3301 cggaaaaacc aaaagcggac acaactgaaa atacacgctc aactgatacg aatgccgctt
     3361 taagttttgc tgatttttca aaagccgttg ctgagtatcc taaattttac acagaacaaa
     3421 atgaacatga agtagttatt aatgaaaaaa atgctgatga acctcgtaaa ttagcaacta
     3481 tttctatgac tactgaaaat gagttcgtta tcgaaaaagc actcgaacgg aaatacaaag
     3541 tcaaattgac tttgattcct ttgatcgaac aatttgcagc gacagcgatt actgatcgat
     3601 aaaaattcta tttgtcataa aaggctggga tttaaaatcc cagccttttt agtcatataa
     3661 taatttctac agcaagtaaa aagaaattcc ttctaacatc aggctcaaat atcagcttga
     3721 cctgtccaac aaatcaccta tgatcctaca atgttaggcg acttctcttc tacctgaaga
     3781 tatcttggag ttgcctgatt tcttaagatg cctaacctta actataaaaa attctaataa
     3841 taaacatatt ttctgataga gaaaaaa
//
LOCUS       DAYYNQ010000162         3861 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000162 DAYYNQ010000000
VERSION     DAYYNQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.167"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBJ1_002846"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACHBJ1_002846"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM9431716.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMVVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACHBJ1_002847"
     CDS             complement(2260..2511)
                     /locus_tag="ACHBJ1_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM9431717.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBJ1_002848"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACHBJ1_002848"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM9431718.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACHBJ1_002849"
     CDS             3461..3679
                     /locus_tag="ACHBJ1_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431719.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1067 a          819 c          715 g         1260 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac aaccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt a
//
LOCUS       DAYYNQ010000163         3725 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000163 DAYYNQ010000000
VERSION     DAYYNQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.168"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..152
                     /locus_tag="ACHBJ1_002850"
     CDS             69..152
                     /locus_tag="ACHBJ1_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9431720.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHBJ1_002851"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="ACHBJ1_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9431721.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1277..2140)
                     /locus_tag="ACHBJ1_002852"
     CDS             complement(1277..2140)
                     /locus_tag="ACHBJ1_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM9431722.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2142..2414)
                     /locus_tag="ACHBJ1_002853"
     CDS             complement(2142..2414)
                     /locus_tag="ACHBJ1_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM9431723.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2431..2640)
                     /locus_tag="ACHBJ1_002854"
     CDS             complement(2431..2640)
                     /locus_tag="ACHBJ1_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM9431724.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2718..3614)
                     /locus_tag="ACHBJ1_002855"
     CDS             complement(2718..3614)
                     /locus_tag="ACHBJ1_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM9431725.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1141 a          653 c          579 g         1352 t
ORIGIN      
        1 ttttgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctacc gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttata gatgtaatca cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
     1081 aggtataggg cacctctaat aactaccaat taattcacct caaaatgaaa ttagaaaaaa
     1141 acagcgaaat ttttaaatct atttcttatc gatacaaatt cctcgtaggc gctcgggacc
     1201 ccaacatgag gaaatgtttt tataaaaaaa gagtagttac cgttttttgg taactactct
     1261 tttccttttt taaggtttaa atacctggaa gtttaggtgt tttgggtatt ggtggtgtag
     1321 gtagctgcaa gctttccatt ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt
     1381 gattttgaac cattttttgc tctattctct ccaaagtcgg ttgaatttct ttccctgata
     1441 ctttacgatt taattctctt tctaaggtct ctttcggact aatggaaggc gtttctaagc
     1501 ttgaatagag tttcactcct tctctattgt aaagccttat atcgctaaat aagcccgttt
     1561 tatgaagggt ttctaaattg gtcggtaagt ttttgacaac aatatcatgc gcttgttttg
     1621 gtgttgccct agctgtcatt ggatcatctg catacatggt ttcatatcgt tcaatcgttc
     1681 ctaagtatga ttcaattttc ggtactgcca tgacgtatat ttttgtttca taacctttag
     1741 attgaagcat tgtggctgtt tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca
     1801 ctaaattata gccttgatcg cttaaacggc taataagtgc ttctgtcatg cgattcgaat
     1861 aaggggtaac gtgttttact acgtcttttt catagagttt cactaattcg tcaaaatttg
     1921 ggtgctgttg tttaaaggta tcattatcaa tgacaataac attcccttgt gtttcttcaa
     1981 aaattgctga tcgcaaactg gttttccctg accctggttg cccaccaagt aaaaaagcgg
     2041 ttggcgattc aaccgctagt ctatttttag ttagtcgttc aacattcttt tctaaacgat
     2101 cttcaaattg cttttcggtg aagtcagtaa tattcgccat gttaagccac tttctcttta
     2161 ttcaaaattt gttttgaata ttgattcatt gaccgccaat actcatgaat agcttgtaat
     2221 tcttcaaatg gttgttgaaa taaatcaact tcttgtgcca cttctaattg atttaaaaga
     2281 ttcacttcta gtaaacgagt atttttagta tctagttcat gattcaaaac ataatttaat
     2341 acccgattat aaacacttgc cgataactca ttaatgattt caatttcaaa tgttttttca
     2401 tacgtaactg ccatttcatt ttcactcctt ttaaagtttg tcaggtaaat atttccgtat
     2461 atattcttct aaggcttgat ccataacttc ttttacgttt ccgccattct ttgctgtttc
     2521 gatttttatg atatggtgca agtcggcacg aacacgaacc gtcttatccc ccattatatc
     2581 tttttttgcg ctgattggcg tatcatttcg ttttgctttc tgtgcgccta aatttcccac
     2641 gatcgctcac ttctttctat ttcttcttac tatttatttt atcatcacct atcacaaatc
     2701 acaagtgatt tgtgattcta ttctttttca ttttctaatt gaatgattcg ctcaatcatt
     2761 tcaaaaaata cgttctcata catagacaaa acttttttgt catagccttt gtgttctgta
     2821 atgccatttt tagaccaagt acttacttta ttacttcgct tgataatatt tcggaaaacc
     2881 aaattatcct ctttatgttc tttgtacagt tcttccaggt ttgattttat cgttgcgctg
     2941 tccgtatcaa ctaaataagg aacaaaacca atcatatcta gtccagggtt aaactgttct
     3001 tgcaaatcaa tcaaatagga aatatagttt tgaatgttgt ttgtactttc ttcttctgct
     3061 tgtaaaggga tcataacgta atcactcgcc acgattgcat tatttgtata aacgcttggc
     3121 gttggtacag tatcaataat aataagatca tagtcacttt ttaaaggtgc taaaagagta
     3181 gcaagcaatc tactttcatt ttcaaacgtc catgagcgag ttaattttgg cagtaacatc
     3241 aaatcaaacg tgccagggat caagtctaaa ttatcagtca aatgaataat agaagaagcc
     3301 aagtttccat ttttcaagcc ttcataaaaa ttgacacgtg gcaattctac ctcaaaagtt
     3361 tttgctaagt cttttgtcaa tgttgcctgt aagtccttat cgatcattaa aacttttaaa
     3421 ttcaatttgt ctgtcaagta agcaaacata gtcgataatt tggactttcc aacaccacct
     3481 ttaaagtaat tattcaagat aataattgct tcatttttat tgtttaaaat acgtcttaat
     3541 tcttcaagta tttttagttt ttccttctcc ataactacgc cccattcttt ttttgtatag
     3601 taatattgta tcattaagaa tcacaaatca caagtgattt gtgattaatc acttgtgatt
     3661 tgtgataagt gatttgtgat taatatataa aagccctctt taaagggctt ttataggttc
     3721 tgttg
//
LOCUS       DAYYNQ010000164         3718 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000164 DAYYNQ010000000
VERSION     DAYYNQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.169"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHBJ1_002856"
     CDS             complement(<1..353)
                     /locus_tag="ACHBJ1_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431726.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHBJ1_002857"
     CDS             complement(613..1161)
                     /locus_tag="ACHBJ1_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431727.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHBJ1_002858"
     CDS             complement(1162..2019)
                     /locus_tag="ACHBJ1_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM9431728.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="ACHBJ1_002859"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="ACHBJ1_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="ACHBJ1_002860"
     CDS             complement(2581..2910)
                     /locus_tag="ACHBJ1_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9431729.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="ACHBJ1_002861"
     CDS             complement(2987..3259)
                     /locus_tag="ACHBJ1_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM9431730.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="ACHBJ1_002862"
     CDS             complement(3259..3522)
                     /locus_tag="ACHBJ1_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM9431731.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYNQ010000165         3575 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000165 DAYYNQ010000000
VERSION     DAYYNQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.170"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACHBJ1_002863"
     CDS             complement(<1..2232)
                     /locus_tag="ACHBJ1_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM9431732.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACHBJ1_002864"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACHBJ1_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1025 a          782 c          552 g         1216 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaata
//
LOCUS       DAYYNQ010000166         3547 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000166 DAYYNQ010000000
VERSION     DAYYNQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.173"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1838)
                     /locus_tag="ACHBJ1_002865"
     CDS             complement(<1..1838)
                     /locus_tag="ACHBJ1_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM9431733.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="ACHBJ1_002866"
     CDS             complement(1835..2575)
                     /locus_tag="ACHBJ1_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM9431734.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3547)
                     /locus_tag="ACHBJ1_002867"
     CDS             complement(2565..>3547)
                     /locus_tag="ACHBJ1_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM9431735.1"
                     /translation="ESIKNIFVSYFTNIYFAALNIWTGFKLTLINIWNEVVNQAKSIW
                     LNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNI
                     KDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEI
                     FNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRW
                     MRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISA
                     SETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         1121 a          673 c          422 g         1331 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atatttcaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtagtaatct ttcgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattga gtgcagcaaa ataaatattt gtaaaataac ttacaaatat attctttata
     3541 gattccc
//
LOCUS       DAYYNQ010000167         3515 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000167 DAYYNQ010000000
VERSION     DAYYNQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.174"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1359
                     /locus_tag="ACHBJ1_002868"
     CDS             28..1359
                     /locus_tag="ACHBJ1_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM9431736.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1325..1675
                     /locus_tag="ACHBJ1_002869"
     CDS             1325..1675
                     /locus_tag="ACHBJ1_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431737.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1767..2186)
                     /locus_tag="ACHBJ1_002870"
     CDS             complement(1767..2186)
                     /locus_tag="ACHBJ1_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM9431738.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2187..2531)
                     /locus_tag="ACHBJ1_002871"
     CDS             complement(2187..2531)
                     /locus_tag="ACHBJ1_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM9431739.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2881..3477
                     /locus_tag="ACHBJ1_002872"
     CDS             2881..3477
                     /locus_tag="ACHBJ1_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM9431740.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          923 a          640 c          641 g         1311 t
ORIGIN      
        1 taaagaaaat gaaaaagaag gtgtgtaatg gaaaacgaaa ctgacgttat ttatattcat
       61 cctcaaaaac gaatcgtttc acagaaaaga aagtattttt atcttggttt tacaggtgtt
      121 ttttttcttt ttattggctt gctttctaat actcctactg ataattggag tggattgcta
      181 actattctta cctcgccaag caatcttttg acagattatt ttgctttagg tgttttcgga
      241 agcgcattca taaatgtagg gattttgacc ttactttctg tgcttttagc ttatcggcac
      301 aaggtgattt taaacggtcc cttatttgct tcgattctaa cagttaccgg tttctctttt
      361 tttggcaaaa acctttataa ttctatctca atcattttag gtgtgtatct atacgctgta
      421 tttgtcaata aacctttttc tcagtacatc atgatcggac tgtttggtag cgtattatca
      481 ccggtcgtta gttatatcac ttttggtatg cgttttcctt tactgatagg tattttattg
      541 gggaatttag ctggcatagc aattggtata cttcttccgc ccttagctgc tcaaacttta
      601 gttttccatc gtggattcac actttataat attggattca cctctgggtt gattgcgatg
      661 acttttactg ctgttttgag gttattcagc tattcaatcg tcgaaaatac attggttttc
      721 aatgaatatc actttcctct catttggatt atttttggct ttttttcgct gacagtcgga
      781 attggtttct attataattc ctttcggctt tcggggattc gagaaatttt cgattcatct
      841 ggaaaattga caactgattt cattgctaat tctggtattg gtgctacttt gatcaacatg
      901 ggcttagttg gtctgatgct gtcttcttat gttttgctgg ttggcggtca gttaaatgga
      961 cctgtcatcg gtgctatttt atctgcggtg ggtttcagtg cttttggttg tcatttaaaa
     1021 aacagttttc cgatactagt cggaatattt attgcttctt tgtttggaac ttttcatgag
     1081 atcaccagta ctggcatgct tgttgcagct gtgtttggga caggtcttgc gcccataagc
     1141 ggtttttacg gcagttttta cggtgtaata gctggtgtgc tacatattgc tcttgtccat
     1201 aacgtcagca ctttgcacgg aggactaaat ctatataaca gtggtttttc aactggtttc
     1261 gttgcaggta tccttgtacc gatactagat aactttaccg ctgtcagaaa ggaaaaaaag
     1321 acacttggaa aaagaattat caaaaagaat catcgatgag cttcttactt atttatattc
     1381 ccatgaaata actgaaatac gtattggagt agaatttgct gaggaagggc ttttcataga
     1441 gatacagggt acggcagaaa ctcagccaga agatttagat gtacttgctg atttgctgaa
     1501 caatcctcgg gaccctagta tggaaatcta ctatcaagaa ctattaggta atccccgaca
     1561 tgatcgggaa gattatcatc tgttaggtag tttgattgat gacgtggaaa tcatgtatgc
     1621 ccagccgatt atcaatatta agatctttcg acgtgcttct cattcctatt attaacccta
     1681 ttttctattt ataaaagact gaatcaaaaa agcagctcct gaaagttcga ttaaaatcta
     1741 ctttcaggag ctgttcaagc ttgacattat tgattttcca atccttcata cacttcatct
     1801 gttacaggtt ctaaaaactc tgaattacca gcagtgatcg ccaaatgttc aaaccaactg
     1861 tcttttgtcg caccatgcca atgtttaatc cctttgtgcg ttacgacgac atctccagcc
     1921 attaatcgtc ttgctggctt tccttcttcc tgataccagc cttctcctcc ggtcacaagt
     1981 aagatttgat agccgtctgt atggacatgc cagtgattgc gacaacctgg ttcaaaagtt
     2041 acattaccta caccaacaga aacttcggga tcattcacta acatattcag atagctttgc
     2101 ccggtaaata cttgagcgta agcttcgttt ttttcacctg ctggaaaaat cacgccattg
     2161 tgtatttctt cgtgttttgt catcatttac gcctctttct tatcttcgct attttcttct
     2221 aaatagattt cttttgctaa actaaaagct gaccaagctt ttggccattc tacataaaaa
     2281 gctaaatggg taatgatttc tgctatttct tcactagtaa tgccattttg cttgccaatc
     2341 ttcagatgag ctggcaattg ttcaaaattt cctcctgaaa ttaatgcaga tatggtgatg
     2401 atactacgct gatgagcaga taattcttct ctggaccata cttgtccaaa taaaacatca
     2461 tcatttaatt ctgcaaattt tggtgcaaaa tctcctaatt gatctcgtcc tgcggtttgt
     2521 ttttctgcca tttctcttat tctcccttca cgactgttag gtgattcaac tatggttcta
     2581 aggcaacttt attttctatt gtttcttcgc atttcatttt aaatgtagtc tttcagcctg
     2641 tccaatgctt agtttattca gtcgatatgc tttttaagca tagttgctaa cgcttttttt
     2701 attttaaaaa aaacaagtta ttaaataagt ctttagttgg atgttttttt gagatcaatc
     2761 ttttatactt atgatgaact tacaaaattg tttgttttct gtcatatcaa aaaatggcgc
     2821 agttagttgc ttttctagta aagtatagtt aacaaagaac ctctaaggag ttgatgaata
     2881 atgaatattg ctgcctctcg aaaactgata aatttcatat ccattatcgg attaggccta
     2941 tcaatcgcgc tgacgattta ttttattaat ttaggtgttt tcaaagattt gaatgcgtta
     3001 agaggattag taggtgattc catcatttta ggaccgatta tttttgtctt catccaaatc
     3061 ctgcaagtag ttattccaat cattcctgga gggatcagta ctgcagctgg tgtcctgata
     3121 tttggccctt atgctggatt tatctataac tatattggaa tctgcattgg ttcgattatc
     3181 atttttctgc tagggcgtcg ttatggaaag ccattcatac tttccatgat tagcgataag
     3241 acatataaca aatatattgg ctggctggat aatcaaaacc gttttgagaa gctgtttgct
     3301 ctagctattt ttcttccagt tgctcctgac gatgcgttat gtttgatggc tggattgaca
     3361 aacatgtcag tgaaacgata tacactaatc attttgattg ctaaaccact gtcgattttc
     3421 ttatatagta tggccttgat ttatggaggt caatatctaa gtggtcttct tggataatat
     3481 ttattttgta aaattagtca ttgattgaca atgtt
//
LOCUS       DAYYNQ010000168         3400 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000168 DAYYNQ010000000
VERSION     DAYYNQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.175"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1438)
                     /locus_tag="ACHBJ1_002873"
     CDS             complement(<1..1438)
                     /locus_tag="ACHBJ1_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM9431741.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKL"
     gene            complement(1502..1861)
                     /locus_tag="ACHBJ1_002874"
                     /pseudo
     CDS             complement(1502..1861)
                     /locus_tag="ACHBJ1_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(2039..2359)
                     /locus_tag="ACHBJ1_002875"
                     /pseudo
     CDS             complement(2039..2359)
                     /locus_tag="ACHBJ1_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2475..3065)
                     /locus_tag="ACHBJ1_002876"
     CDS             complement(2475..3065)
                     /locus_tag="ACHBJ1_002876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431742.1"
                     /translation="MNSLRVAKAIVGMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLF
                     PHEDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEI
                     QYEGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMEN
                     NINYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
BASE COUNT         1051 a          629 c          472 g         1248 t
ORIGIN      
        1 ataatttatc gtccgatgtt aattggattt gataaatcca agaattcaaa aagatagacg
       61 ttatttgaag ataataatag tcaaattcta tttgaaaatc tctctctatt tccttaacat
      121 actgtctaaa tcgaatctct tgataggtgg gatatgttaa agctgaatta ttttggatat
      181 caacattctt ttgtctaaaa tatctatcat atttctcaaa aagctgctgc ttatcagtgt
      241 ctttcttcca cgtttgataa agatactcta taagttcgtt gatattaaaa acttcgtcta
      301 ctttttccaa atcatccatt tgaatatatt ttaattcatt ctgtcttttc ataaccttca
      361 tgccgttgaa attcacccat tgtggtaaat gatacatctg aatataaagt tcttccaaat
      421 catctaagaa ataaaaggca ttggcacagg tcaattgaat gatatttttc aaattgccaa
      481 cattttcact aaagcgatag tttaataaaa aatctaataa ttgttgagat atcagtattt
      541 tctttgctag tctttcttct tctgatttaa aaaatatctt gattaactgt tttctttcat
      601 aaagagaacg actgtctaat gttggaagtg ttagctttac aggtattctt ctcaacaaag
      661 ttgataatag tttatcctca ggtggttctg atgttgcaaa tataatccag caacttgatg
      721 tgtaccagtt ttcattatca cccatccgat gaaatttcct cgtatccata aactgaaaaa
      781 gtttctcctg acattcattg ctcaatgcat gaatttcatc taaaaataag actccattat
      841 tagctaattc tgctaaaccc tttcttccat cttcggctcc tgtatatgca ccttttacat
      901 gcccaaaaaa attggacaag aaaagttctg gattattggc atattcacta caattaactg
      961 aaatgaaaga tgccttctct tctatataat tattggccaa caaatcctta tacacataat
     1021 ttgctaaata gctttttcca gttccagtct cgccatgaag taaaatgggt aagccatttg
     1081 gcggatagta gattgctgct ttaatttgag caattacatt tcgcagactt ccttgacttc
     1141 cgataaccct ttctattcca accctaaaag agctatcata ccatttttcc gcttcctctg
     1201 aagagctaaa aatttgttga tttggaactt tctccggtaa agaaaagaag attactggac
     1261 gagtattgat ttttccaaat tctccatctt ttacgaattc attcaaatat tgagagacaa
     1321 tattacgact aacatttaat ttttcagata ttttctgtgc tgtattgttc ccccatttat
     1381 ttttaatctc agagttctct atgacataat tgacaatgcg ttcttttaat tcttccatca
     1441 cagcacctcc tcgacactat tcgaattttg ccgacagtat ttttccataa atatgacttg
     1501 ctaaaaaggc tttgtttaga tcattcaatt ggaacgttgc ttgttttgct tgatccgcta
     1561 agacgttgaa aagatactct ggcgaaatcg cataccccaa aatatcaacg attgttgcaa
     1621 tcagatcttc ttttgattct tcatctgaaa gtaattcacg atacaacatc gtttgtttgc
     1681 ttactgtgtc atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac
     1741 ggtcggataa atatttatag aaaatcagcc ccaataaata atttttatat tctgacgcat
     1801 ccattttact acggagatta tctgccgcac taaataactt ctgatttaat tctgcaccca
     1861 aaaagataca ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact
     1921 cagttttaga aaaagcaaaa aaataaagaa acttattctt gataacaagc tacgtgacgt
     1981 atatccctaa cacaaaaata gccatatcct ttggtttgga tacggctatt tgatattact
     2041 cattcaatta tcaaatcaca gtaggtgtga atctcatcgg gatcggcaat atctacttct
     2101 gattaggatc aaaggtgatt ttctcaataa aggtgtgata caaagcttta acttgcttct
     2161 tctcactctg gataagaaag caatccaaac tagtcaatat ctgttggaca tctttgactt
     2221 gaatcggttc cccttgttgt tgaaagatcc ctaagatttc attctctcga atttgtaact
     2281 gtcttcgttt ttcagtcaat tcatttaaac gatcttttaa catcggaaac aattcgggac
     2341 tatcttccaa agcagcttcc tgtttttgct ctaatagtta gattttgcgt accattttct
     2401 tcttttattt tccttcgatt gctgatttta atatagtcta aaggaaattt atatttattg
     2461 tattctttat aacttcaaga gaacttcgtt tgagtattac tgtatttttt taattccata
     2521 agaaaattag ttattacctg ccacctaatc agcaaatctg aacctgagtc aatataatta
     2581 atattatttt ccatctccaa cattgtaagt actgttggtg ttaatatagg gactttgtac
     2641 aattgtattt ttttgatttt atcatctgca caaaggagat tttttaaatc taatccttct
     2701 tttatttcaa aagttgaatt ttttaaatct ataagagtaa aaccaattcc ttcatactga
     2761 atttctgaaa atcgaatttt tctcccagtt gatagatcaa ggggcagaaa accttttatt
     2821 acattgtgag tgtcaccatc atttacaatg tcattaggaa ttttgatttt agaattctgt
     2881 ggagcaccta ttggaagata ataattcatt ataattttgt agtcttcatg aggaaataaa
     2941 gtacttttag gattgctaag atattctcgt accgaatcta agaatggctt atccattgaa
     3001 tcttcatccc catttaaact aaaaaacatt cctacaattg cttttgcaac tctaagtgaa
     3061 ttcattttct ttattgaatg tgttaaatca gacttaccat tctttaaact tgtattgtta
     3121 taaaggaaat ttttattgtc ttctgcaaat ctaatatatt cctcaacata ctcactacca
     3181 aaaaaactat tacatttact acataacgta ttggaatggc aacagttttt ttgacaaatt
     3241 ttataaggtg cagaacttct ttccgtatgc tattccgatt gtccttgaca atgagcctcc
     3301 tcggatgtga cgatcttcgg caggagctaa cagttaaagt tagactgcct cgctagcgtt
     3361 agcacatccg agctcattat caaggattcg ctgcgccaat
//
LOCUS       DAYYNQ010000169         3391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000169 DAYYNQ010000000
VERSION     DAYYNQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.176"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..492)
                     /gene="greA"
                     /locus_tag="ACHBJ1_002877"
     CDS             complement(13..492)
                     /gene="greA"
                     /locus_tag="ACHBJ1_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM9431743.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(628..1803)
                     /gene="mltG"
                     /locus_tag="ACHBJ1_002878"
     CDS             complement(628..1803)
                     /gene="mltG"
                     /locus_tag="ACHBJ1_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM9431744.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            2007..3176
                     /locus_tag="ACHBJ1_002879"
     CDS             2007..3176
                     /locus_tag="ACHBJ1_002879"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM9431745.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         1055 a          656 c          534 g         1146 t
ORIGIN      
        1 ctttcagtca gactacgctt gttctacttt gacgatttta actaacatat cgccacctgg
       61 agtagcaata gttacttcat catttttacg tttgccaatc aatgcttgcg caataggaga
      121 atcattggaa attttgccag aaaacggatc tgcttcagcg cttccgacaa tcgtgtactc
      181 ttcttcttct ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc
      241 tgcatctaca ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt
      301 gattcttcct tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga
      361 aagatctccg aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt
      421 ttttagttct tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac
      481 tttctctacc atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt
      541 gaggaatata gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa
      601 aatgatcaat gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc
      661 catatgttct tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa
      721 ataataatag tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt
      781 gtcaaaaggt ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc
      841 tgtatcttca tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc
      901 tgactgaagt ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg
      961 atcattttct tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac
     1021 agtcaaattt ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt
     1081 caccatttca atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg
     1141 gaataagtaa ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag
     1201 ttctgggaat ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa
     1261 ttctttcttg tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta
     1321 cccttctgga atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc
     1381 ttgcaactgt ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc
     1441 ttgaaagcca gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat
     1501 aatattgtcc ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc
     1561 gacttggact aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg
     1621 atacacggta aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat
     1681 gatcttcctc acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc
     1741 tgctgtagct gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga
     1801 caaaacaact cctccttact atcaagttcg taactttatt cattatacat gatatttgaa
     1861 ttggcgtgaa tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact
     1921 ataagattaa aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa
     1981 aaagaaagga gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat
     2041 attcattttt aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta
     2101 ttcttggatg gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag
     2161 ctcaaaaaaa gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac
     2221 tattaagaaa aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca
     2281 tggaaaaagg ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt
     2341 attatcagat tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg
     2401 agcgtgggca cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca
     2461 gccagcaaat tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt
     2521 ctccaaccat accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat
     2581 ttacagatta tcagacctct cattatgatt cattgaactt taccatattg actggaattt
     2641 tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt
     2701 taggattaca gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat
     2761 cctatcaaat gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga
     2821 tccataacga attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt
     2881 ttacacaagt tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg
     2941 aaaatgcgaa tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt
     3001 cgcaaggaaa tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg
     3061 ctatcatcat ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc
     3121 taagaaacca aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg
     3181 atgaaaagat agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact
     3241 aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc
     3301 caaataaaca gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat
     3361 ttctctttac caacattatt tgacaaaggg c
//
LOCUS       DAYYNQ010000170         3280 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000170 DAYYNQ010000000
VERSION     DAYYNQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.177"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHBJ1_002880"
     CDS             complement(63..2555)
                     /locus_tag="ACHBJ1_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM9431746.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHBJ1_002881"
     CDS             complement(2562..3029)
                     /locus_tag="ACHBJ1_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM9431747.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="ACHBJ1_002882"
     tRNA            complement(3165..3238)
                     /locus_tag="ACHBJ1_002882"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
BASE COUNT          882 a          723 c          537 g         1138 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa
//
LOCUS       DAYYNQ010000171         3209 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000171 DAYYNQ010000000
VERSION     DAYYNQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.178"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(429..1043)
                     /locus_tag="ACHBJ1_002883"
     CDS             complement(429..1043)
                     /locus_tag="ACHBJ1_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9431748.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1649..2026)
                     /locus_tag="ACHBJ1_002884"
     CDS             complement(1649..2026)
                     /locus_tag="ACHBJ1_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431749.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2326..3207)
                     /gene="galU"
                     /locus_tag="ACHBJ1_002885"
     CDS             complement(2326..3207)
                     /gene="galU"
                     /locus_tag="ACHBJ1_002885"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM9431750.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          977 a          659 c          496 g         1077 t
ORIGIN      
        1 ttatttctat tagaaaaagg tatcctgttg gacaattttg ttatatcagg taaacttcat
       61 tgcaataatg ataacaaatg tactataatt actttgtaaa gagttttggg gaagactctt
      121 tcatcaacgt gtattaagag acaagaaaga atccatatgg attctttctt gtctcttttt
      181 ccactacttt ttatttttct gacactacat attcttataa ctcatttctc ataactgtga
      241 aacaacttat aaaaataaca aactttaaac aaaacaagct taaaatcaaa gatgatccat
      301 cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc ctcattaata atcgttttct
      361 ttttttcaaa cgctccattt ttatgtggaa cattattttt ttaaggaact ttgtcaacat
      421 gtggataact attttccact tgtgtcctgg actagctgtt ttgctgtaaa atacaaaact
      481 tgctgcagca aaaagatcgt tgcaagagag gctgttccgc agatcgctgg atctagattt
      541 ggcaaagtca agactgcatc atgtaaattg gcgtggttgt ccaaaaccaa atcagcaaat
      601 tcaaataatc gcaaaccact gctatgttgc gatttgatac ttcgtgacaa atcaaatcca
      661 gtaacaatga ttaatggatg accattttct ttgatccact gagctagttc gatgattgcc
      721 gggtttcttc cttcattcga aagaagaaga aaaatgtctt ctgatttgac agaaagctga
      781 tcaataattg cctgtcctgc accttctagc gtttcataga cacctccggc tggattttca
      841 atcaatatac ttggtacaag atccggggtt tgatcccaaa attcaaatgc tacaccactt
      901 agtgtgcggc tagcatatat ctgcaatcta ccaccttgat taatttgttt cgcgatccaa
      961 gtgccagctt gaagcatcag cggctcttct atccttaatt tctcttccaa ttggtgataa
     1021 tagtcaaaat aacataaact catttatctc tcccgcctag ctatacttga catgaataga
     1081 aacagctgcc taatctaagg aagtacataa aaaaacagta gttagacagt aacacatgtg
     1141 gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat acgcccaata ccttgacata
     1201 gcaaataaaa caacgtgcag accatggaaa atatctacat aggggtagaa ttttcaccct
     1261 agattaggta caataaatta ggtcgatgca ctgcattgac tatggcaaaa tgatcggtca
     1321 gggtgtatgt gtgtgactgg tcattccctg attcttagcg ccaactaagg gtcagggagc
     1381 ctttttttat tcagctgttt ctatctgaat ttattgtaag taatctctta tccaagtgca
     1441 agcaagaaaa tacaaaaaaa cattggtatg atttatatat atataaataa cataaaaaaa
     1501 ggaatggctt atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga
     1561 aaaaagcttt tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt
     1621 atcttttttt gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt
     1681 cgagagaatc tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact
     1741 ttcttttcat atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata
     1801 cgcaagcttc ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg
     1861 ctgcatacca gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa
     1921 atagactaat aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact
     1981 tgagtatatc aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc
     2041 tttttttata attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt
     2101 catttctctt atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca
     2161 taatattttc tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa
     2221 ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt
     2281 taataacaaa tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct
     2341 tagcttttta ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg
     2401 aagtccatat tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt
     2461 tcctttaaac tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt
     2521 tagctgtatc tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg
     2581 agtcagtaga taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt
     2641 ttctacaaac ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat
     2701 tccgtattta gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt
     2761 ttcttcataa tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc
     2821 ccccagcatg acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc
     2881 ccccagtcct cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc
     2941 aactaaagcg agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt
     3001 tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc
     3061 gattcccgaa tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg
     3121 aagcatttct tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc
     3181 aggaatgact gcttttttga ccttcactt
//
LOCUS       DAYYNQ010000172         3169 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000172 DAYYNQ010000000
VERSION     DAYYNQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.179"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(35..403)
                     /locus_tag="ACHBJ1_002886"
     CDS             complement(35..403)
                     /locus_tag="ACHBJ1_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM9431751.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            553..1035
                     /locus_tag="ACHBJ1_002887"
     CDS             553..1035
                     /locus_tag="ACHBJ1_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM9431752.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1072..2082
                     /locus_tag="ACHBJ1_002888"
     CDS             1072..2082
                     /locus_tag="ACHBJ1_002888"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM9431753.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2197..2910)
                     /locus_tag="ACHBJ1_002889"
     CDS             complement(2197..2910)
                     /locus_tag="ACHBJ1_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431754.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            3060..>3169
                     /locus_tag="ACHBJ1_002890"
     CDS             3060..>3169
                     /locus_tag="ACHBJ1_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9431755.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         1074 a          518 c          621 g          956 t
ORIGIN      
        1 tcctcgccag taaaaggttt attcgtataa agctttatcg ccaaaattta tcatgacctt
       61 ttttatcttc aatattaaat gaaatcaatt gttcaatcaa ttcccagttg attacacttt
      121 tccaaggaac tttgatgacg ttatccgtat atacgtagcc tgcttcttgg atattttttt
      181 caagggcacg aatcgctgct ttttccggtg cgatagaaaa atgattttta gcaaaactaa
      241 atgcaataat aaaggtacca tggtcagtaa acataggttg gttccatttt acagttgtgt
      301 ctagctgggg atacgtttct gttatcttag caaaaaggtc tttgatcttt tgttgatgtt
      361 ctttttcttt gatctgattg atgatcggct gaaatttttc cattctaaaa cgcctccatt
      421 aagtggtttg atttatttta ccagaaaaac aatagttatg tataaaaaac gaacaaaaca
      481 caagtaatgt tgtaaccgct atatttatat gataagatgt ctcaaataat aaaatagaaa
      541 agagacataa aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag
      601 ttgtatcatc atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa
      661 ggatttacag gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac
      721 gtatatctga cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt
      781 aaaaggcttt ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc
      841 gctgtttctc cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta
      901 tcaaatgaaa aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg
      961 gttgacttaa acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac
     1021 gaaaaaaaca attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt
     1081 tatccattat tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca
     1141 gaggaacacc attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa
     1201 gtagttactg gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa
     1261 cgagcagatg aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgcct
     1321 tggggaggag aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca
     1381 aaatggatag taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca
     1441 gggatagcca ctgcacatgg aaccaacctt attgatttaa gaggagagta cttggatgaa
     1501 acgacagcaa tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa
     1561 ccttctgaaa agtatcaaaa agagtggccc catgagcatt catcatcttc tgttttccat
     1621 ctcactgaac caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga
     1681 ttattaggtg gctgtattga tacgatcagt catttaatcg gtacccctta tggagatgtc
     1741 caatctttta aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaattgt
     1801 gagctatcgt cagttgattt aagaagatca cttgttcaga tgaaactggc aggatggttt
     1861 gatggcagct cggggatcat gttcggtaga agtgcagcag aggatttgtt agattataaa
     1921 atgaaggatg tatatgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt
     1981 gattgtggtc atgtaccgcc acaactgaca ttgatcaatg gtgcctatgc agaagtagcg
     2041 gtagaagata gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt
     2101 ggagtttatc aaagaccaac aaatctaaat agaaaagaaa atgaaagaag ctgacagtat
     2161 tttgccagct tctttttgta tgatctatta gaaattttaa ttatccataa ctatcacatt
     2221 gatcagctgt ttcacatcat ttggattacc tgtaggaata gctactacag atgcattatc
     2281 aataatttct tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat
     2341 ttgccaatgt gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac
     2401 taaaactccc tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc
     2461 actccctgca tcataagggg cgataggtgt tcctgcagga taatgaataa catgtagttc
     2521 gaaatcaccc gatgtatctc taaaagtatt acctctaatt gctagccaaa cacgtgcgta
     2581 ctcaatttgt agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga
     2641 agttgtgtct attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga
     2701 agtattgggc tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga
     2761 tttctgagta cttgattttt cagctgaact ggtttgtttg attgaacttt tgtctgtggt
     2821 cgcagatgga tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac
     2881 caacaataaa ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca
     2941 gtatagcata atcctgaatt tttgaatccg taaaaccttg tctatggata ggctacaatt
     3001 aactagacag aaaaattaag gtgtgtagac tagaagaaaa cataccagga ggaattttta
     3061 tgtctaagag aacacgaaga actttttcac aagaattcaa gcaacaaatc gtcaatcttt
     3121 acttagctgg aaagccacgt gtagaaatca ttcgagaata tgaactaac
//
LOCUS       DAYYNQ010000173         2889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000173 DAYYNQ010000000
VERSION     DAYYNQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.180"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            563..>2889
                     /gene="esp"
                     /locus_tag="ACHBJ1_002891"
     CDS             563..>2889
                     /gene="esp"
                     /locus_tag="ACHBJ1_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM9431756.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1048 a          441 c          566 g          834 t
ORIGIN      
        1 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
       61 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcagaaatg
      121 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
      181 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
      241 ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc
      301 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
      361 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
      421 tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag gttagtaaga
      481 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt
      541 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
      601 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
      661 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
      721 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
      781 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
      841 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
      901 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
      961 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
     1021 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
     1081 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
     1141 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
     1201 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
     1261 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
     1321 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
     1381 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
     1441 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
     1501 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
     1561 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
     1621 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
     1681 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
     1741 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
     1801 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
     1861 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
     1921 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
     1981 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
     2041 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
     2101 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
     2161 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
     2221 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
     2281 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
     2341 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
     2401 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
     2461 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
     2521 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
     2581 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
     2641 aatttcagat gtctataata agtacttcgg taatgcaagt gttgatgcaa acactggtat
     2701 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
     2761 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
     2821 taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
     2881 caccgcagg
//
LOCUS       DAYYNQ010000174         2663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000174 DAYYNQ010000000
VERSION     DAYYNQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.181"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..690
                     /locus_tag="ACHBJ1_002892"
     CDS             <1..690
                     /locus_tag="ACHBJ1_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM9431757.1"
                     /translation="RNNVIFTLSLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVK
                     IVRSAYSGNYQAASREFIIELCQQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKN
                     EWKEDLLAYLSEKSLASEPYLNLSKKELASQLTMPKRTLDKILNELREENKVFYKVTR
                     GRNGGMIIASVKTLFARVIQLSKEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPL
                     KEVNLFEVDTG"
     gene            825..1121
                     /locus_tag="ACHBJ1_002893"
     CDS             825..1121
                     /locus_tag="ACHBJ1_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM9431758.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            1222..2073
                     /locus_tag="ACHBJ1_002894"
     CDS             1222..2073
                     /locus_tag="ACHBJ1_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431759.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            2094..2306
                     /locus_tag="ACHBJ1_002895"
     CDS             2094..2306
                     /locus_tag="ACHBJ1_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431760.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          935 a          370 c          557 g          801 t
ORIGIN      
        1 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
       61 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
      121 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
      181 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
      241 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
      301 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
      361 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
      421 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
      481 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
      541 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
      601 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
      661 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
      721 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
      781 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
      841 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
      901 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
      961 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
     1021 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
     1081 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
     1141 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
     1201 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
     1261 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
     1321 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
     1381 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
     1441 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
     1501 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
     1561 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
     1621 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
     1681 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
     1741 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
     1801 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
     1861 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
     1921 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
     1981 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
     2041 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
     2101 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
     2161 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
     2221 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
     2281 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
     2341 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
     2401 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
     2461 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
     2521 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
     2581 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
     2641 aaattgatcg aagatgtgac gaa
//
LOCUS       DAYYNQ010000175         2652 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000175 DAYYNQ010000000
VERSION     DAYYNQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.182"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            677..763
                     /locus_tag="ACHBJ1_002896"
     CDS             677..763
                     /locus_tag="ACHBJ1_002896"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM9431761.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1289)
                     /locus_tag="ACHBJ1_002897"
     CDS             complement(1065..1289)
                     /locus_tag="ACHBJ1_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431762.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="ACHBJ1_002898"
     CDS             1655..1939
                     /locus_tag="ACHBJ1_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431763.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="ACHBJ1_002899"
     CDS             1942..2358
                     /locus_tag="ACHBJ1_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431764.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..>2652)
                     /locus_tag="ACHBJ1_002900"
     CDS             complement(2495..>2652)
                     /locus_tag="ACHBJ1_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM9431765.1"
                     /translation="PALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT          873 a          495 c          426 g          858 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg at
//
LOCUS       DAYYNQ010000176         2587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000176 DAYYNQ010000000
VERSION     DAYYNQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.183"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACHBJ1_002901"
     CDS             <1..1412
                     /locus_tag="ACHBJ1_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9431766.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2587
                     /locus_tag="ACHBJ1_002902"
     CDS             1546..>2587
                     /locus_tag="ACHBJ1_002902"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM9431767.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTA"
BASE COUNT         1016 a          363 c          519 g          689 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcac
//
LOCUS       DAYYNQ010000177         2581 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000177 DAYYNQ010000000
VERSION     DAYYNQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.184"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2260
                     /locus_tag="ACHBJ1_002903"
     CDS             <1..2260
                     /locus_tag="ACHBJ1_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM9431768.1"
                     /translation="IDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYFN
                     SNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVE
                     QLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVL
                     AKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLET
                     FELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQS
                     IVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIEL
                     LISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIFE
                     IDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEH
                     YRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELS
                     VSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDIL
                     IFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNS
                     ANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENG
                     NMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASS
                     YLFKFKQKRSDG"
     gene            2395..2523
                     /locus_tag="ACHBJ1_002904"
     CDS             2395..2523
                     /locus_tag="ACHBJ1_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM9431769.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          987 a          339 c          495 g          760 t
ORIGIN      
        1 aatagacaaa ttagatacga aagacattgt caattggctc aatcaagacg aagaattgac
       61 agatttatta tcttcggtaa aaaaagaatc aagtgcaagt cagcaatcga actggagtgg
      121 attgtacttt aattcaaatg agtatgtgaa atacattttg aataaaactg catggattaa
      181 gattgatgga gaaaaatata gcccgagaca aattgtgcta tatgaaaagc tccaggcaca
      241 cgttccggga ttgtatggag tttctgaaca aagcttaatg gaactcttgg ggcaacgcat
      301 tgttgaacag ttggatttca gaaaaaatat ggcgatgttt tcggatgatg aaataagagc
      361 tatattagtt gagttaccaa aattcgataa aggcgaaata agtcgcagat tatataatga
      421 acttataaga aataaaaaag gaatgaatcc tacttattct accgaaggat tatcagtctt
      481 agccaaagat ggcttctttt atccaaacaa agaattaagg tatgctgata agcgactgcc
      541 taaatcaata gaaaaacaaa tgcgttttat ttacgtgcct gaaaaagcta gtacaactac
      601 gatatatgaa tggcttgggg ttgaacgctt caaaacaaat ttaaaattgg aaacatttga
      661 gttattggaa gattattctg aggatttcaa aaaagagatt gatgatatca aaactgcggt
      721 attatctacg atagatgaca atagtaagaa tgttagttca ttaaaacgta ttcagattat
      781 tccatgcagc aaaatatctg ctaaagatgt tgagcaagat ggtcagtcaa tcgttttgga
      841 agattattat tttgtggaaa gtaaaggaaa attctactta aaattacctg ctaattctat
      901 tgaaattaat caattacgtt tgattgatac gttttcttca acaatcgtag acatattcaa
      961 gcaggtgtta aatctaagct tagatttgaa tttgattgaa ttgttaattt cccgagatac
     1021 ggaacataaa caaagaaaaa taactgaaga attcggtgta gataagtgga atgaatcgtt
     1081 tgagctattg tttagcgaaa atgcgttaaa taaaaaagtt aatgactttt tgttatcaaa
     1141 tggactaaat gaagaggaat atcctaccat ttttgaaatt gactattcat atgcattaaa
     1201 agaagaagag tatcaattgt tggcgatagc tctagaaagc atttcaaaag atattgagga
     1261 tttgaatgga ttgtcagaat tgattcattt ggatattcgt gaattttgga aactgaaatt
     1321 aagatcttat ctagataaga aagcggaaca ttaccgccag attttatata gcaatgcaaa
     1381 taaaagcggt gatgaaatac tcaaagaaac attcctagaa aaaattgata gatacaaaga
     1441 ttatcaattt gagatttctg aaattccaaa ttcagttcaa gtcgacattg agaaagaaag
     1501 taagaagaga tttccagagc taagtgtgtc aattactgat gaaattgaca ttgatggcct
     1561 gtatgataat aatgtcgaag agttagtcac ggttgcagct attacaatag atgattttga
     1621 ttattatatt agaggtcatc aaaaagtttc ttccttactg tattttggta ttccgtcccg
     1681 tttaagagaa gatatactga tattcgttac taaagaaaat gatgttgatt tgaacggaga
     1741 aaaatctggt atctctattg aaggaacatc aactgttcaa accattcttg aaccatcgaa
     1801 tagtgttagt aggaaaggta agaacacacg aaatggagaa agaagtaaac gagattatga
     1861 taatagaaac tccgcaaatg atgatgctgg tgaaacagca gaacaaattg cttacgctga
     1921 attgactcag aatcaaaaac ttaatgtaat ttggaatacg aaatattcta gtaaatcttc
     1981 agatcgtaat aagcagccac caaacggaat tgtttgtgat atgtgggttc atgacccaga
     2041 gaatgggaat atgtactttg aggtaaaatc atcaattaca gaatttgaaa tgtcgattaa
     2101 tgaatataat tctatgttag ataaccctga ctcttacgag gtcgttttag tcaatcgtga
     2161 taccaaagag ataagtcgtc ataaatttga tgaacttgat ggattgaaac aggcaagtag
     2221 ctatcttttc aaatttaagc aaaagagaag tgacggttga gtgatttgtt tccacaaacc
     2281 tattcaatct atcaactcaa aaatcataaa caaccattat tatagtagga agatacagag
     2341 tgtactctca acgcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac
     2401 caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa
     2461 atggaaacac ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa
     2521 tagacgtgct aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt
     2581 a
//
LOCUS       DAYYNQ010000178         2561 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000178 DAYYNQ010000000
VERSION     DAYYNQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.185"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(845..988)
                     /locus_tag="ACHBJ1_002905"
     CDS             complement(845..988)
                     /locus_tag="ACHBJ1_002905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431770.1"
                     /translation="MENAITVITNSFFSSIKYLKTLFVCQADQCSFRLGRTLISSQVM
                     IEV"
     gene            complement(1241..2407)
                     /locus_tag="ACHBJ1_002906"
     CDS             complement(1241..2407)
                     /locus_tag="ACHBJ1_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM9431771.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT          837 a          484 c          337 g          903 t
ORIGIN      
        1 tcggagttaa acacttctgt cccagcctct tctgtccaaa ttattctaag atttctcttt
       61 accaacatca tttgacaaag agcttctttt ttgaatacta tattcgttct gtttttcatc
      121 ttcaaatacg aactcatccg cagaaatatt tgtcacaact ccatgaatct tcttttcatt
      181 accacctata taccacagga ttcctacaca gaaacaacaa aaaaatagca taaaataggt
      241 tttctttatt ttatatccct ccttttagat taaaagctca cagaaaacgc tttctaaaaa
      301 gacaatagaa taatcataat ttttcatttt gtattattta tacaaataat acgcatgtta
      361 ctgaaaaatg atatggttcc ctactttttg agataagtta aataaaatct gtcatcgata
      421 aaacagctag tacagtaatg ataaatagta agaacttaaa atattttgga tttttatttt
      481 tgaaataatc gaagaaaagt aaaagtgata acgaaatagc aaacaagatt atatctataa
      541 gattattcat tgagaaatcg ccccatatgg aactctattt tgacctgaca caccgagaca
      601 taaagcaatc atatcctctg cattgtttta tactattaga actatagcta cacctattgg
      661 atgctactgg ctctaagatt aatgctaata cagtaacatc aatacctaaa ctagcagaaa
      721 ctctatttgc atcttcaatt tcagatgcct ggactgtcgt gactagcgcc gtagtagaaa
      781 aatttactgg tactaacaaa gatgcagaca cgacttttat ttcacttatc ctttatccca
      841 taacctaaac ttcaatcatt acttgtgagc tgatcaatgt tcttccaagt ctgaaagaac
      901 attgatcagc ttggcaaaca aataatgttt tcaaatactt gatcgatgaa aaaaagctat
      961 tggtaataac agtaattgca ttttccattt tcgtgtcaga tacttcgctt acagctggta
     1021 aacgatcaat cccattgtca aattttggat ttttttctga tgatgtcttg ctttagttca
     1081 taaaataatc cagcaataaa ttcctcgcaa ggatagccaa cttcattttc ctaatcggaa
     1141 tttttaaact tatcggtata gatacagatg caatgagaac aaacatttat ttcgagtcac
     1201 catctaaaat attttcctcc atgtacccgt tcattattat ttagttatca ccaaagatat
     1261 ctctggtata aactttttct tcaacatcct ttaaaatatc ttcaaaccga ttagatacga
     1321 tcacatcaga aatattttta aattcattaa tatctttcaa tacttgtgaa tgataaaatt
     1381 catcttcatc caatgtaggc tcgtaaacaa ccacttcaat tcctttggct ttaatccgtt
     1441 tcataattcc ttggatcgac gaatatcgaa aattatctga accggccttc atcgttaaac
     1501 gatagattcc aacaacattt ggttttttcg ccaaaatctg tccagcaata aaatctttcc
     1561 ttgttgtatt tgcatcaaca attgcactga tgatattgct tggtactttt tcatagttgg
     1621 ctcttaattg ttttgtgtct ttgggtaaac agtatccacc atagccaaaa gaaggattat
     1681 tataatgcga accgatccgt ggatccaaac cgacaccttc aataatttgc ttactatcaa
     1741 gcccacgaaa ttctgcatac gtatctaatt cattgaaata cgccactcgc aatgccaaat
     1801 atgtattcga gaataattta atagcttctg cttcagttga gtgagtcaaa agaactgatg
     1861 catctttgtt aaatgcgttt ctttgaaaca tagttgcaat ctcgcgacca cgagcagaac
     1921 attctccaac tataattctt gatggatata agttgtcgta caaagctttt ccttcacgca
     1981 aaaattcagg tgaaaaaatg atgttttctg taccaaattt cgctttaatt tcatttgcat
     2041 agccaactgg aactgtcgac ttaataatga atgttgaatc tggacgaatt gctaaagcct
     2101 tctcaattac atcttctact gttgatgtat taaaatagtt tttcttttcg tcataatctg
     2161 ttggagttgc aataatcaag taatcaccga agtttactgc atcagtaaaa tttgacgtaa
     2221 attcgacatt taagtcatcc cgttttaaaa attcatgaac ttctttatct tctattggtg
     2281 agatgccatt ttgtaacatc gtgatttttt cttccacgat atcataagct ttgacctgct
     2341 ctttttgtgc taataatagt gaatttgcta gaccaacata gcctaatccg aaaactgaaa
     2401 ttttcatatt cttccatacg ctccaatcaa atttcatttt atagtatttt agctgttatc
     2461 cttttgaaca tattccttat tcaatcaata ttatttaata gaaaaaattt ttttgcttca
     2521 aaatcatcga ggttcgatat ttaataaatt catttttatt t
//
LOCUS       DAYYNQ010000179         2505 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000179 DAYYNQ010000000
VERSION     DAYYNQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.186"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..1020
                     /locus_tag="ACHBJ1_002907"
     CDS             133..1020
                     /locus_tag="ACHBJ1_002907"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM9431772.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            1106..1336
                     /locus_tag="ACHBJ1_002908"
     CDS             1106..1336
                     /locus_tag="ACHBJ1_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM9431773.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1393..2358)
                     /locus_tag="ACHBJ1_002909"
     CDS             complement(1393..2358)
                     /locus_tag="ACHBJ1_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9431774.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT          850 a          402 c          490 g          763 t
ORIGIN      
        1 cgattgaacg aatcaaaata aaattaaatt catttaaaag ataaaattca aacatttttt
       61 atgaaaaaat ccttgtttct agcgtatact agcatagaag caaggatttt tgatttttag
      121 gaggtcgaaa aaatgaaact tggttttatc ggaacaggcg tcatgggaag tgctgtcgca
      181 cgccatctac tagaagcagg acacgaagta gctgtctata atcggacaaa agcaaaagct
      241 gatcctttag taacagaagg agctatctgg gcagatacac caaaagcggt agcagaacaa
      301 agtaatattc tctttacaat ggtaggttac cccaaagatg tagaagaaat ctactatgga
      361 cagtcgggga ttttttctgc agatatctca ggacatattt tagttgattt gactacaagt
      421 acaccaagcc tggcagaaaa aattgcaaaa acagcaaaag aaaaaggcgc agatgcgtta
      481 gatgcaccgg tatccggtgg tgacttagga gcaaagaatg gtacactgac gatcatggtc
      541 ggtggagaag aagccgttta tgatcaagta ctgcctttat tcaaagaatt tggtacgaca
      601 tttacattgc atggttctgc tgggaaaggt cagcatacaa aaatggctaa tcagatcatg
      661 attgcaggta cgatgacagg aatgacggaa atgcttgtct atgcacaaaa aaacggactt
      721 gatttaaaga aagtgattga gacgctttca ggtggaagtg cagctaactg gtctttaagc
      781 aattattctc ctcgtatttt aaaagaagat tacacacctg gtttttttgt caaacatttt
      841 atcaaagatt taaagattgc attagaagaa gcagaaaaaa tggatcttgt attgccagca
      901 actacccaag cattgaaact atatgaagag ttggcagata aaggttttga gaatgacgga
      961 acccaagctt tgatcaaact ttggtggcct gaaggaaaaa taccagaaaa aaaatcataa
     1021 aaaacgagtt tttttagaga aaatcaatgc aagtaggcta tgcttttgat acaatataac
     1081 agaaataaga aaaggaggac ctccgatgac acattcacaa ttagtcgcaa tcataaaacg
     1141 actggaggct atggtcgagt cagcagataa tgaactgcaa gttcgccgtt ttgaaaaaga
     1201 aggcgtagag aagtgtacag taacttatga taaagcgacc gatacatttg aattaactga
     1261 aacagatact catcagcaat acgaatttga taatatcgat atcgtagcga tggaaatcta
     1321 tgacttaatc caataaattt taggataatt gacatttgaa gttgaagatg cctaaaagaa
     1381 gccgagaagc aactaattta gttgcttttc agcttctttt ttgtttaaca gttcaatttc
     1441 aaactgttct ttttctaatt ttttgcggta actgatcaag atttgtacag gatcgatttc
     1501 ttcttttgac aattgacgtt tcaaagataa atgagcttct ttcttttcat aatagaagtc
     1561 aggagaaacg tcaccagagg taatcattgt ttctgcaagt tgaccataac aaacgaggta
     1621 gtgctttttc tgactctcat ctgctgctaa tggcatgctg aaggtgagcg atggtgttct
     1681 taaaaactgc ttttgttcct ggatccgtct aataaaataa gtaactaatt cgatatcttt
     1741 ttgaagcata ccggcttttt cgaaattcaa ctgatagatg aaatgttgtt gtcttttttt
     1801 caagtaagtc agtagctttt tatttcggcc ttgaaacagc ccttggagtt cctttttttt
     1861 ctgttcaaaa gtcatttcct gtaggtcagg aagctggact cgcaaagcta acaaactaat
     1921 ttcagacaac caaggcattt gataagtttc ttccattata cgcaaaatag acggcatttt
     1981 tttatagaga cgaaaaggcc cgtatgttcg agccgtagga gtatctgtta atacaaatcc
     2041 cttattggta atatgtacat aattataatt actgaaataa ttcatttgtc ggttataaaa
     2101 aggacggtaa tgttggatca attgacactc gagcaataaa gcatctaatt ccgtgtctac
     2161 tacgacgaaa tcaaaatctg ttatatgatg aatcatacgt aatacttttt ttgaatgctg
     2221 tttattctga tgaaaatagc tgcttactct atttttgagc cgttttgctt taccaacata
     2281 gatgacctta ccatctcggt ctttcataag atagacaccc ggcagcatgg ggagagtttt
     2341 cattttttct tttaacatag gatctccttt tacgctttga ataaaaagac tataacaaat
     2401 tttgaaataa tggacaagtt ttagggaatt ctatagttct gttcacagaa aatactttga
     2461 taatcaaaat atgtgagata gatgattttt ctaataaaag aaatt
//
LOCUS       DAYYNQ010000180         2495 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000180 DAYYNQ010000000
VERSION     DAYYNQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.187"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1386
                     /locus_tag="ACHBJ1_002910"
     CDS             <1..1386
                     /locus_tag="ACHBJ1_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431775.1"
                     /translation="ICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAI
                     AEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIV
                     RLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEA
                     LSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTN
                     LFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFL
                     LVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKH
                     EVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKH
                     GLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVT
                     KKEINKKNENK"
     gene            1373..>2495
                     /locus_tag="ACHBJ1_002911"
     CDS             1373..>2495
                     /locus_tag="ACHBJ1_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM9431776.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLF"
BASE COUNT          832 a          374 c          424 g          865 t
ORIGIN      
        1 atttgtacga ttctgattag tttttattat ggagaaaagt tttttaaaaa gatagatgct
       61 gcttttttat ttagttgttt atatactttt gcaacttatc gaacagttga tatctattat
      121 cgatccgcaa ttgcagaagc aatcgctctt acgattatcc cagctgtttt atattatgct
      181 tatggattga tattcaatag agaaaaaaaa gtcatcgctt tatctgtcag tataagtcta
      241 cttgtgtata ctcatgtctt aactacacta ttgtgtagtg cattgattgc ttgtatgatt
      301 atagttcgtt tgtttttaag caagcaaata aaaaacgata taaaatttgt ggtcgtgaat
      361 ttagtaaagg ctgttttttt gacttgtgca ttaactagct atttttggta tcctatgttg
      421 cagcagatga attatcaaga aatacacagt cccttcaaaa cagatttgca gctagaagca
      481 ctttctattt cagatagtat cattcaagcg ttaaataacg atattacaac atacactatg
      541 gggttattag gattggttgc gttgattttt cctttattca tactgaaaga aatggagaaa
      601 aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg tatgcgtaac taatttattt
      661 ccatggtttt tgtttcaaaa aacaccgata agcttactcc agtttccttg gcgtatctta
      721 ggtatccaaa tctttttttc ttcaatgatc ataacgcttg tttttatgaa aaagactatg
      781 aataaaaaat attcatggtt aatcattggc gctactgtgg tgtttctact cgtcacgtct
      841 atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa aacacggtca tattgttatc
      901 aatgaagaaa cactcccacg ttatacaaca tcagatatgg gaggattata cgattacgcc
      961 ccaacagaag cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa agtaggagaa
     1021 tcgtgggaaa aagggaagta taaagctgca gataataaca ttacttacac agtggcaagc
     1081 aacaaaaagc aaaaagtgac agtaccagta tatgcttatt taggtacaca ggtaaaagta
     1141 aacggtaaag tcgtaaatga ctcttataaa aaacatggct tagtgaatgt ggatgtaaat
     1201 gctggagaaa ataagataga aattacaaca aaatatactc caactgctct catggcttta
     1261 tggatcagta ttctgactta ttgtttagtg gtgtattcaa gttttaaacg atttgcacct
     1321 aataagttga aagattctcg atatgtgact aaaaaggaga taaacaagaa gaatgaaaat
     1381 aaataaaaaa gtagtaatat tgagtggatt aattatagtt agtagtatat attctttcat
     1441 ctttttcaaa aatacacaga gtatttatac cactggagat ctttcttttc acttatctcg
     1501 aatcaagggg ttatcgacta tttttaccag tccgattaat tatgagacat tcaattatac
     1561 tggatatggt gtaaattatt tttatccctt tctgactttt tttccagctg taatgttata
     1621 ttggatgaca aaaaatttga ttgtaagcta tattatttac gtttggttat tgaacttgtg
     1681 tacaacactc gtagcttatc attatggcga gcggttttta aaacaaaaaa aagcggcatt
     1741 tttatttagt tgtctctata ttttctctgc ttatcggaca gtagacatct actatcgttc
     1801 cgcaattgcc gaagctatcg caataacact tgttatccct gtattatttt atgcttatca
     1861 aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag ctagcattga gtatgagttt
     1921 gctagtgtac agccatgtac tatcaacatt aatgagtaca gcattaatta tcatttttat
     1981 ttttattcgt ttggtttcta aaggtttcaa gaatgcagat tttattgcta tatttaaaaa
     2041 attatttagt gcggcaggaa tgacattggt tctgacgagt gctttttggt atccgatgtt
     2101 tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg acaaatttat atgctcatgc
     2161 atctaatgtg ttcgatagtc tgactgaagc tatgaacaac gacttaacta cttactcaat
     2221 gggactagtt ggtctgttat ctttatgtat tcccctgatt ttatttaaaa aattaacaaa
     2281 catagaaaaa aagatttatt atggtacgtg tttgacttgg ctagctacaa ctagtttggt
     2341 gccatggtat cttctgcaaa atacgccagc caaattgtta caatttcctt ggcggatctt
     2401 aagcttgcaa ataatattct cttctctgat attaacgatg atatttttca aaaatagaag
     2461 atacaataag acaagagaac tattttattt attta
//
LOCUS       DAYYNQ010000181         2459 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000181 DAYYNQ010000000
VERSION     DAYYNQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.188"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACHBJ1_002912"
     CDS             5..1150
                     /locus_tag="ACHBJ1_002912"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM9431777.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACHBJ1_002913"
     CDS             1321..1779
                     /locus_tag="ACHBJ1_002913"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM9431778.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACHBJ1_002914"
     CDS             1938..2408
                     /locus_tag="ACHBJ1_002914"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM9431779.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          823 a          460 c          459 g          717 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgcctt
//
LOCUS       DAYYNQ010000182         2456 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000182 DAYYNQ010000000
VERSION     DAYYNQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.189"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..2306
                     /locus_tag="ACHBJ1_002915"
     CDS             153..2306
                     /locus_tag="ACHBJ1_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM9431780.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          839 a          428 c          543 g          646 t
ORIGIN      
        1 gatatgtaac tttaagtgat tgctgtgaaa caaaaagttg attttatctt gaaattaggt
       61 aaagaatttc ttgctccgcc cattttttct tttttattaa gaagtttttg ggtacaatga
      121 agcatgaatg taaataggcg gaaggatagg aaatgatgaa agattttatg aaaaaaatat
      181 taaatacttc ttggttagaa aaaataagaa aaggtgcaca agaacctaag aacaaaacgt
      241 ttcgccggtc ccatgttcct tttcgcttaa attttctttt tttcattata tttacgttat
      301 ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt gaacggcgaa gagatggagg
      361 cgcgagtgaa gtctacttct acgattacgg tccaagaaag tactcctcgc ggaatgatct
      421 acgactccac aggaaaagct ttagttacga ataaagcgaa tcaggccatc acatatacga
      481 gaggaacaca agttacggct aaagatttgc ttgaaatcgc aacgaagctg aactcgctga
      541 tcgatgttcc ggtagatgaa aatctgacag atagagataa aaaagatttt tggctggctg
      601 atcccaaaca tttgaaagaa gccacaaaaa gactttcggc aaaagaaaag caattaggga
      661 acagcgaaca atatagtgcg atggtcaata aagtcacagc tgatgagatt aatttaaacg
      721 aggagcaatt aaaaattgct accatcttta aacggatgaa tagtgcatat gaattaaaca
      781 ctgtctatat caaaaactct ggtgttactg atcaagagtt agctgttgtg gcagaacgcg
      841 cttctgaact tccaggagtt tcgactggca cagactggac acgagaatat aatgcagcaa
      901 gttcactgaa aagtattctt ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg
      961 aagcagagaa atacctagca aaaggttatt ctcggaatga ccgtgtagga caaagttatt
     1021 tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg
     1081 ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt
     1141 taatgttatc tatgaatgca gaattccaat caaaagtaga agaaattttg aaaaggaatt
     1201 atcaaacatt gatcaacaac ggcaaagcac agtattcgcc tggtgcttat gcagtggcta
     1261 tgaatcctca gacaggagaa gtgctagcaa tgactggttt cagtcatgaa caaggttcca
     1321 aagaaatcac cgagaatgct ttaggcacga ttactagcgc ctttgatcca gggtctgtag
     1381 tcaaggctgg tactttgaca gcaggctggg caagtaacgc gatcagcgga aatcaagttt
     1441 tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa
     1501 gcggacaagt tgctcttgat gcagtcaaag catcggaatt gtcttctaat acgtatatga
     1561 tcaaagtggc gttaaagatg ttaggactag actatacacc tggaatggga ctacctagtt
     1621 tagatgaaga agctaaagct tatcagcaat tgagagactc tttcaaagag tttggtcttg
     1681 gtacaacgac tgggatcgat ttgccgaatg aatctcctgg gatctcacgt tctgttgatt
     1741 atatgaagaa gttcaatgcg gataatggca aagaatggta tgctccaggt aactttaccg
     1801 atttggcatt tggacagttt gacacatata caccaatcca attagctcaa tatgcttcaa
     1861 cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt caaagcgatc tatggcaatg
     1921 acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg cacaacagta gaaaatacgg
     1981 tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg attccaccaa gttgtccatg
     2041 gtaccgatcc ttacacaaca gccaaaccat tagccagtgc gaaaatggac ctttccgcaa
     2101 aaacagggac tgcagaaacg gttgcggaag gtcatccaga tattgcgaca gtcaatagta
     2161 atatcgtggc atacggacca acagacaatc cggaaattgc catcagtgtt gtcttgccga
     2221 acttattaga cgaacaagac cacataaact tgacgattgc taaagaaata atggacgcgt
     2281 attatgatat gttcatggca aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag
     2341 tatgaagaaa aattttgtga aaatagcttc tcacattttt gcttaaattt gacttcatac
     2401 cgagaaactg gcttttgaac accgtttact cggcaaaaaa ggttgtgaca aaaatt
//
LOCUS       DAYYNQ010000183         2438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000183 DAYYNQ010000000
VERSION     DAYYNQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.190"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHBJ1_002916"
     CDS             316..570
                     /locus_tag="ACHBJ1_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM9431781.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHBJ1_002917"
     CDS             571..888
                     /locus_tag="ACHBJ1_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM9431782.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHBJ1_002918"
     CDS             1447..2382
                     /locus_tag="ACHBJ1_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431783.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          884 a          345 c          458 g          751 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagagaaaaa
     2401 tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAYYNQ010000184         2274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000184 DAYYNQ010000000
VERSION     DAYYNQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.191"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..530
                     /locus_tag="ACHBJ1_002919"
     CDS             12..530
                     /locus_tag="ACHBJ1_002919"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM9431784.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            658..1341
                     /locus_tag="ACHBJ1_002920"
     CDS             658..1341
                     /locus_tag="ACHBJ1_002920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM9431785.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1650..1723
                     /locus_tag="ACHBJ1_002921"
     tRNA            1650..1723
                     /locus_tag="ACHBJ1_002921"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1684..1686,aa:Arg,seq:tct)
     gene            1882..2241
                     /locus_tag="ACHBJ1_002922"
     CDS             1882..2241
                     /locus_tag="ACHBJ1_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM9431786.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          762 a          352 c          457 g          703 t
ORIGIN      
        1 agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta
       61 tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt
      121 atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac
      181 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
      241 gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      301 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      361 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      421 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      481 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      541 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      601 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      661 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      721 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      781 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
      841 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
      901 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
      961 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
     1021 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
     1081 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
     1141 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1201 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1261 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1321 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1381 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1441 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1501 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1561 aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat
     1621 ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg
     1681 ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa
     1741 actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc
     1801 aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc
     1861 atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca
     1921 attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt
     1981 agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag
     2041 aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct
     2101 tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat
     2161 ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca
     2221 ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca ggaa
//
LOCUS       DAYYNQ010000185         2228 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000185 DAYYNQ010000000
VERSION     DAYYNQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.192"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..483
                     /locus_tag="ACHBJ1_002923"
     CDS             <1..483
                     /locus_tag="ACHBJ1_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431787.1"
                     /translation="KKESVIILVYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELI
                     TKSTEATTEELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIK
                     RSNLCCASYTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA
                     "
     gene            complement(544..909)
                     /locus_tag="ACHBJ1_002924"
     CDS             complement(544..909)
                     /locus_tag="ACHBJ1_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM9431788.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(985..1230)
                     /locus_tag="ACHBJ1_002925"
     CDS             complement(985..1230)
                     /locus_tag="ACHBJ1_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9431789.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1300..1554)
                     /locus_tag="ACHBJ1_002926"
     CDS             complement(1300..1554)
                     /locus_tag="ACHBJ1_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431790.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1718..2041)
                     /locus_tag="ACHBJ1_002927"
     CDS             complement(1718..2041)
                     /locus_tag="ACHBJ1_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431791.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          842 a          368 c          387 g          631 t
ORIGIN      
        1 aaaaaagaaa gcgttattat tttagtctat tttcaggggg ggaaccttat gataaaaaaa
       61 ctatttttac tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca
      121 actgaactga tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat
      181 cacagtgact acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt
      241 aagcatcgat gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag
      301 atcaaaagaa gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta
      361 aaccacgtca ggagatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag
      421 tacgctttta gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct
      481 tgatctaaga tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt
      541 atattaggta agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac
      601 ttcatcgtgt tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc
      661 ttcgcatgat ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg
      721 tttttccatt tcatccatga caagctgatg atattggaat aatctataag gagaatagtg
      781 aaaaacataa tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca
      841 ttcccgatgt tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca
      901 taatcgcata gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa
      961 aaccagcacc aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat
     1021 taggtaatca acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg
     1081 aatttcttct ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga
     1141 aacagctact tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt
     1201 atccagtaaa atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt
     1261 ataatgacta tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg
     1321 tatcaaataa agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa
     1381 aacactatcg ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg
     1441 aagagtcaaa atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact
     1501 tgcgaccagt gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg
     1561 gactcctcaa cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa
     1621 tgctaggaaa ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg
     1681 ctgataagca ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat
     1741 tgatatctaa gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa
     1801 gaatgatgat cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg
     1861 catatctttt tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt
     1921 gaaaaatcat tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag
     1981 ctgctgccgc aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca
     2041 ttgagaataa ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac
     2101 aaaatagtta tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt
     2161 gaaacttgtg agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat
     2221 ttatgatg
//
LOCUS       DAYYNQ010000186         2198 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000186 DAYYNQ010000000
VERSION     DAYYNQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.193"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1872
                     /locus_tag="ACHBJ1_002928"
     CDS             10..1872
                     /locus_tag="ACHBJ1_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM9431792.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1872..>2198
                     /locus_tag="ACHBJ1_002929"
     CDS             1872..>2198
                     /locus_tag="ACHBJ1_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM9431793.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKN"
BASE COUNT          788 a          331 c          436 g          643 t
ORIGIN      
        1 gagatgataa tgcagcagct aacaaaaaga gaaaggcagc ttttgattta cttggtggaa
       61 atgtctgaac cggtaacaat caaaaagttg gctgaaaaag aaaatgttag tgttcggacg
      121 ataaaatacg atctagatga tataagagag tggctgaatg agcggaagca agtcctatat
      181 agcaaacgta gtcagggaat ttggatgaat attagtagct ctgatcgaat caaattaaaa
      241 agcgaattga tggatgttaa tcgttttgaa atatttgcag atcaaagtct ccgaatcaaa
      301 cgtttactaa ttatgctttt agtaacaaaa gaagctatta ctggaatgaa aatggccaac
      361 gcattagatg tcagcaaaga tacgatcatg aatgatttag atgtgctgga aaataatttt
      421 cttaaggaag gattgacctt gaatcgtcag gcgcgaaagg gtttttggat cataggagaa
      481 gaacgtctag ttcgattaac gattgaagag attcttcaaa aagagttcac ggattatgac
      541 atttataaat taatgtcatt gttattgaat ggcggtgaga ctgaatactt tgaaatgtat
      601 tctgctactg ctacacctat ccaagaggta tttaatcaag taatcattcg aatgcgacac
      661 ttactggaat ttgaaaatct agagaaactg aattacgcgg aattactaaa tattctgatt
      721 cgagtgacaa ttgctaccgt tcgcttacgc aaagaggcta cgattggtcg ttatcaattg
      781 gttgctgaac aagaattaga caaaaacgat ctttcttatc gcgtcatgca gcaggtcttt
      841 gattattatc agttgccact gttcgaggat gaatatcatt atatctatag tgatacattc
      901 gacacgattc accagcaaga cgtcatgaaa ctagttgaga atgtgattta taaagtaagt
      961 aaagcaatca accattcttt ttatcaagat ccacaattgt tgacgaatct gtatgctcat
     1021 ttatccatgc gattatcgag aaagaaaaaa tttgtcaatg aatataatcc ttttaaagaa
     1081 gatatcaagc agaaataccc ggaattattt gatgcgatca agcaagcagt tcgacaagaa
     1141 ataagtgaac aaaatattct gatcaatgat tctttcgtgg catatattgc cttacacttt
     1201 ttggtttcct acgaaagaga agaagatcta cgaagcgtta gagccgtcta catttgttca
     1261 acagggttgg gtgtgactag tctgatcaag caaaagatca atgaagaatt aaataatatc
     1321 actatcgtgg catttgcttc agtattgaat gcgcaagaaa tgattgctac caaagatcct
     1381 gatttggtga ttagtatttt tccaattaat ggcatcgatc gtcctttcat caaagtccat
     1441 ccattaccca ctgaacagga cttgaaaaaa attcaacaaa taataaaaaa aatacttgct
     1501 tcttccaatc agcaggaaag gcataacttg aatcttagag aaacgtccat caataaagat
     1561 actttagaag actttagcaa ggaattgatt ttgaaagctt atacgattta tgaacgttta
     1621 cttgtcattt tggaagcaga attgatccaa gaatatcgag aagctttttt gcttcatgta
     1681 ttcttaatgg ttcatcgtat cacatttgat caacaatata tggcagaagg tcgtcaagta
     1741 gaccagatta ttttagacaa aggaacactg gtaggaaaaa tcgaagcagt ttttgcagaa
     1801 aataatttag gaatcaatca gtcagaaatc agtgcattat tctcatatat aaaagaagga
     1861 gcgtcaagtt agtgaaaatg tcagacgagg ctttaaaaat tatcgaggaa agtcaaaatc
     1921 gggaaaagtt aaaacaagtg attgatctaa ttgaagagcg attagtagaa catgctattg
     1981 taccaaccga gttgcagtgg accattctaa ttaatcactt gaatgagatg ctgaaacgtt
     2041 ctcaaagaaa ggaaacgatt cccgtagtcg atcgggattt gttcaaagaa gtatccaaag
     2101 aagccataac tatttctgat gaaatagtta gaagtatcgg taatttaacc gatgatgaaa
     2161 tatatgtttt gtcgattcat tttgaaacag caaaaaat
//
LOCUS       DAYYNQ010000187         2030 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000187 DAYYNQ010000000
VERSION     DAYYNQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.194"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2030
                     /gene="acm"
                     /locus_tag="ACHBJ1_002930"
     CDS             <1..>2030
                     /gene="acm"
                     /locus_tag="ACHBJ1_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM9431794.1"
                     /translation="ADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQPGD
                     YLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVEGWG
                     QFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPEDTK
                     HIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGIQQF
                     LQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAWFKE
                     FNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRADEQ
                     PIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINENDT
                     EGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRLDNG
                     ITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVISVS
                     GQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQEII
                     YTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRNLFS
                     QKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIG"
BASE COUNT          800 a          334 c          401 g          495 t
ORIGIN      
        1 gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat
       61 attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc
      121 caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg cggtgaagga
      181 ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt aacggtcaga
      241 aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc agtagaaggt
      301 tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga aaatacggga
      361 ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa gccagcttct
      421 ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga agatactaag
      481 cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca accagttaaa
      541 atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga aattaatcaa
      601 atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca atttttacaa
      661 gatttcccca gcgctacatt taatttttca gtaactgata attacattga aataactata
      721 ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat catagaaaac
      781 ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt taataaacct
      841 gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc tggtggagtc
      901 aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac agagattggt
      961 atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca aggacagtct
     1021 agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct gcaagtagga
     1081 gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc actaagttca
     1141 aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt acctatttat
     1201 aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg aaccactcca
     1261 agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga accagttcct
     1321 gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga agatatccaa
     1381 cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga
     1441 aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag
     1501 aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga gaaccaagac
     1561 ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc gatccaacac
     1621 aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc aaaatacaga
     1681 gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata cagtacgacg
     1741 attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat atctatacca
     1801 ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga gaaaattaga
     1861 aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag cgaacaaaga
     1921 agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc aaaaagcatt
     1981 cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
//
LOCUS       DAYYNQ010000188         1955 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000188 DAYYNQ010000000
VERSION     DAYYNQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.195"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..392)
                     /locus_tag="ACHBJ1_002931"
     CDS             complement(<1..392)
                     /locus_tag="ACHBJ1_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9431795.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTA"
     gene            complement(389..1042)
                     /locus_tag="ACHBJ1_002932"
     CDS             complement(389..1042)
                     /locus_tag="ACHBJ1_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM9431796.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1330..>1955
                     /locus_tag="ACHBJ1_002933"
     CDS             1330..>1955
                     /locus_tag="ACHBJ1_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM9431797.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          617 a          348 c          251 g          739 t
ORIGIN      
        1 tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt tatttctctt
       61 tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt atctttgatt
      121 tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact actcttggaa
      181 taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt actagaaatt
      241 tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata
      301 aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg
      361 aacatttttg gaaatatctt taattttttc attttctctc caatttatat cctacatttc
      421 ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta
      481 ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc
      541 ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat
      601 ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat
      661 ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag
      721 cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg
      781 catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt
      841 tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga
      901 taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa
      961 taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat
     1021 cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta gatattatac
     1081 agcatattat ggctctataa tatctagtgt tggtgacacc ttctgttgcc aatacttttt
     1141 cttttttcta ataaatgcaa acagacacct tgaaatcctt tagaataaag ggttctataa
     1201 tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa
     1261 ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg
     1321 agtcgctcta tggataatca tactagaaaa ttactcgtat taacagataa acatttattt
     1381 tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa
     1441 gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc
     1501 aacggttcct atacgacacg tactcagata ttgaaagtca aagaaaagtt gactatttta
     1561 gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg
     1621 gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat tctcatagaa
     1681 ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc ggatgggacg
     1741 gtgacacgga tcttgcgtga agcaacgaag cattaccagc cacgtatgaa cttccttccg
     1801 acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc catgagtttt
     1861 atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg cagactttac
     1921 aaactaactc aatacttcct gcgctttcct agaaa
//
LOCUS       DAYYNQ010000189         1949 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000189 DAYYNQ010000000
VERSION     DAYYNQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.196"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHBJ1_002934"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHBJ1_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM9431798.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHBJ1_002935"
     CDS             853..1041
                     /locus_tag="ACHBJ1_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM9431799.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHBJ1_002936"
     CDS             1054..1611
                     /locus_tag="ACHBJ1_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM9431800.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHBJ1_002937"
     CDS             1643..1885
                     /locus_tag="ACHBJ1_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM9431801.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          303 c          401 g          529 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgct
//
LOCUS       DAYYNQ010000190         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000190 DAYYNQ010000000
VERSION     DAYYNQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.197"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHBJ1_002938"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHBJ1_002938"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM9431802.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYNQ010000191         1803 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000191 DAYYNQ010000000
VERSION     DAYYNQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.198"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACHBJ1_002939"
     CDS             <1..553
                     /locus_tag="ACHBJ1_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431803.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
     gene            540..>1803
                     /locus_tag="ACHBJ1_002940"
     CDS             540..>1803
                     /locus_tag="ACHBJ1_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM9431804.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKI"
BASE COUNT          604 a          233 c          280 g          686 t
ORIGIN      
        1 tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat
       61 ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt
      121 atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc
      181 tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga
      241 aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc
      301 ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga
      361 aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata cagcattatc
      421 tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct
      481 atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta
      541 tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta
      601 tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta
      661 ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca attttttcga
      721 ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg
      781 acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct
      841 tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg
      901 tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt
      961 attttacgat taactttaac cattttaaat atttgatttt atcttttata tctacttttt
     1021 tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg
     1081 gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa
     1141 taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct
     1201 ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta
     1261 tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag
     1321 gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg
     1381 gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag
     1441 tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg
     1501 ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta ggctatttct
     1561 ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta
     1621 ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg attttcatac
     1681 ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta
     1741 tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac acaaaaaaaa
     1801 tat
//
LOCUS       DAYYNQ010000192         1793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000192 DAYYNQ010000000
VERSION     DAYYNQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.199"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHBJ1_002941"
     CDS             complement(<1..321)
                     /locus_tag="ACHBJ1_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9431805.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDL"
     gene            complement(322..1230)
                     /locus_tag="ACHBJ1_002942"
     CDS             complement(322..1230)
                     /locus_tag="ACHBJ1_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFM9431806.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1230..>1793)
                     /locus_tag="ACHBJ1_002943"
     CDS             complement(1230..>1793)
                     /locus_tag="ACHBJ1_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM9431807.1"
                     /translation="SYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWII
                     DTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIG
                     EKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTL
                     LEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          597 a          320 c          198 g          678 t
ORIGIN      
        1 taaatcaaat atattatctt ctctttctag ttgtaagaat gaatcacgta tcacttcaat
       61 tttccctaca gtatctgtag ctaattcagg tttacgttct acaattttca cagacggatt
      121 taacttagtc gtcaattcta ctaactcatt actgtccgta ttcaaacaag tcgtgcaatt
      181 atgttcagga tgtttttgtt gatagatatc tatcattgct aaagcgaaat ggactagggg
      241 ctttcctgcc aaatatttca aatttttatt tttaaacccc ttagacccag ctctaccaca
      301 aatagtaaat aaaatttcca tttatttctc ctcagctatt ttcaatactt cgtttgcatg
      361 atataaatca ttccagtttt ctttgcctgt tactacgttt tcaaagaaat atttcaactc
      421 cttctcatac atatcattag cagattcagg catttcttta acaatgtttt cttcaaaact
      481 tattttatta tttaataagt caacattgat acattcatct aaagtaaaca attgaaattt
      541 tcttgtagga actcttccaa aataatctaa atgaatttct ataattttat catcgtatct
      601 caatatatat gttgctaaat cattactgtt aatttcaaga ttcgaatatt taccaaactg
      661 tttttttact gatttaggga aaccaaatag gtttataata taatccaatt catgtataca
      721 gtctaattct actcctcccc ccatagattt aaaggcagaa taattatttc tataatcagt
      781 accttttctc caatcaggaa gataagtaga acaaatagct cttacagaat atactttttt
      841 actttgaata ctattacgaa caacttccat gatattttta tacctcagtg gagcagcaac
      901 ataaatattt ttgtaatcat aattatattg agtaagatca atactctcag aaaaagcagg
      961 tttttctaaa aatatgtaat caactttttc aacaattaca tttagtgttt ccagatgctt
     1021 aaaagtcgga tttgtaataa aagctatatc ataatgtgta tccaaatcgt ctctattttg
     1081 aatatacgtt atttcttcaa actctttttt tccactgcta ctataagctg ttatatcaaa
     1141 attaatagaa ttcttttttc catagcttat tagatttttt agatgtctct ttccaattga
     1201 ccctaaccca aaaaaaatta ctttcatcat taatttacct caatgtcgtt aattaagtta
     1261 ataatatact tatctttttc aaaagtatat tggggttcca ttttcttttc caaaagcgtg
     1321 aaaaattctt taatctcctc taaatatgca tcttcaataa tagtatgtga ataattatta
     1381 tcatgcataa cctcatcgta caattgaata tctgtcatac tagaagtttc ttcattccat
     1441 gaaaatagag aatccgggtt accttcccat tctattaaaa tcttttcacc aattatagtt
     1501 aaatttcttt tagctactct tgaaacaaca tctacactta aagttccaac tataccagtt
     1561 tcatgctcaa taataagagt gaaactgtcc gaataatcaa tatcaagatt ggataatttc
     1621 tgggtcatta cagtaaacga tttaactttc ccaaatgtat ctataatcca aggtaattca
     1681 atagcgaata tttctctaca cccattagtt tttttgtttg aaacaaaaaa attgcgataa
     1741 tcttcccaag gatgccagtc gggtaaatac tgtcctacat ggtattgata aga
//
LOCUS       DAYYNQ010000193         1782 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000193 DAYYNQ010000000
VERSION     DAYYNQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.200"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..1103)
                     /locus_tag="ACHBJ1_002944"
     CDS             complement(156..1103)
                     /locus_tag="ACHBJ1_002944"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM9431808.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1349..>1782
                     /locus_tag="ACHBJ1_002945"
     CDS             1349..>1782
                     /locus_tag="ACHBJ1_002945"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM9431809.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT          570 a          350 c          340 g          522 t
ORIGIN      
        1 tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccga agaagctact
       61 tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca cagtcctttt
      121 tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt caattcattt
      181 ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt ttctaagaag
      241 attttcccaa tatatttccc gatgattcca agacataaca attggattcc accaatgaac
      301 aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa caatgcccgc
      361 aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc gataaatgaa
      421 gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta gttgaataat
      481 ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata agaaatatat
      541 tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta ttctgttaat
      601 tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc gccatcgacc
      661 atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa gaaactgcgt
      721 atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat atcagaagta
      781 gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc aacatccatc
      841 acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc ttctttccca
      901 aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag cttcttgatt
      961 gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa ctcatgggca
     1021 cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc attaaaacat
     1081 gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt atatatggtt
     1141 tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt acgtaaaaga
     1201 acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg gtttatcaaa
     1261 cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa aggaggaagt
     1321 tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg gctttttatg
     1381 gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt cagaaagcga
     1441 atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat attttgcggg
     1501 aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt ttcagcaaaa
     1561 tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata aagaagtagc
     1621 cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc gaaccaatcc
     1681 attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac tgacaggata
     1741 tatgggaaac gcaatagatg gggttgaaat caaacgccaa tt
//
LOCUS       DAYYNQ010000194         1727 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000194 DAYYNQ010000000
VERSION     DAYYNQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.201"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..770)
                     /locus_tag="ACHBJ1_002946"
     CDS             complement(<1..770)
                     /locus_tag="ACHBJ1_002946"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HFM9431810.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVMFALFFSANLFLVHKLSKMNIYE"
     gene            complement(742..1638)
                     /locus_tag="ACHBJ1_002947"
     CDS             complement(742..1638)
                     /locus_tag="ACHBJ1_002947"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM9431811.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
BASE COUNT          603 a          294 c          219 g          611 t
ORIGIN      
        1 ttttcgtaaa tattcatttt tgataattta tgaactaaaa aaagatttgc tgaaaaaaag
       61 agagcaaaca tcactacgtg agaaatcgat gtactgtttc tatcaaaata tgcatcatac
      121 ttcgtattta taaatattga actgatataa ctttgtccaa tagagaatag gagcaagcta
      181 aggattgtaa catatccagt aatcttttta ttaattttta caataaaaaa agggagataa
      241 aaatatgcaa gattatggaa cagtccagcc actaagataa aaaaaatata cttaaatata
      301 gctttgctgc ctcgcaattt agttagatga taaaaagcta gtaatatgaa tatcaatgcg
      361 ctaaacatac gtacttgtat tgcttgtgaa gtaaaaccaa aaataaaaaa caagaacaaa
      421 actaaaaaaa cattagttgt gaataatctg atgacataca ataaagctga gagtaagaca
      481 gtagaaataa tcaataaaaa aatctgatac tgactggaaa taaaggtaca tacgtacata
      541 acagctctga atcccggttc aatactatct gaactaaagt ccataccaaa ttgtgctatc
      601 ccattataca agacagaata tgcataataa tcagcgtttt gtgtgttaaa tgccatgatg
      661 atccacatta tcaaaagtag caccccagac aaaatcttgt tcgatgtact ccattttttt
      721 tgtgaaacac tcttttcttg attaatctcc aatgacgcct ctgtatccat ctcgaacccc
      781 ccttatagcc gctctaaatc tactgagctt atcagtctca aaagctattg caataaaaaa
      841 tttcaataat cttttattca taaggcggga aaattcctta tcacttttat acattctttt
      901 caccaatgtt gaatttcttg caataaaata ctttcgtgtt gaattatgat tgtctatttt
      961 aatttttttt gatcctattt ttaaattttc tccatctcca acgtgatgat aaaaatagac
     1021 ttgatttgtt acataaatgt tatagccatt atttgcaacc cttttacaaa attcaacatc
     1081 aatctgatca ataaaaaatt catccttcat atttccaaca tcgtcaatta attcactttt
     1141 gaaaaacatt ccagaggaat ttgcaaatgc tattttcatg gaaccactag ttgaagcaaa
     1201 gtgttttttt acgtatctcc ctctctcatt tttgcaggta aaagagacag attcatcatt
     1261 gctattctca aagtaattgg gaacaataat cccaatgtcc ttatctttcg cttcaatatt
     1321 ggtaaactct tctaataatt ttaaagaaag attcttttgt atgatggtat cttgatctag
     1381 taaaaggata taatcacatt tctctttttt ggcaatttca atacctatgt tttgagctgt
     1441 cgcaagccca atgttctcat tcattttttt taaaattaag ttttccatca tttcaaattg
     1501 aaaagagtta atattatctg agccgttatc cacgataata agtttgttct gtttctcttg
     1561 tattaacgta tccactaata actttatgtt attatctgga ttgtaggtaa caataattat
     1621 cccaatttta tttttcatcg ttcatcacct tgtacacttc attccatttc attttcaata
     1681 cttgctcaga gaatatagtg gcgtaaattt ttctaacatt ttcattt
//
LOCUS       DAYYNQ010000195         1682 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000195 DAYYNQ010000000
VERSION     DAYYNQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.202"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACHBJ1_002948"
     CDS             200..742
                     /locus_tag="ACHBJ1_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431812.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="ACHBJ1_002949"
     CDS             898..1347
                     /locus_tag="ACHBJ1_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM9431813.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>1682)
                     /locus_tag="ACHBJ1_002950"
     CDS             complement(1377..>1682)
                     /locus_tag="ACHBJ1_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9431814.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          636 a          240 c          324 g          482 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gt
//
LOCUS       DAYYNQ010000196         1648 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000196 DAYYNQ010000000
VERSION     DAYYNQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.204"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1603
                     /locus_tag="ACHBJ1_002951"
     CDS             137..1603
                     /locus_tag="ACHBJ1_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM9431815.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          338 g          552 t
ORIGIN      
        1 ttttatgata ttgccattaa aaattacttg cttttataga aaattagttt atatttctta
       61 aataagtttt ttagttaaat ttttcggtaa agatgtataa tattttctga aaatttaaag
      121 aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc agccgaaggg
      181 attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg gtatgcgtgc
      241 attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat taccacgagc
      301 aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta gtatcgtcgg
      361 cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg gtggagtttt
      421 cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat tgtttgtttc
      481 gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg gcatggtcgg
      541 acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt tttacatggg
      601 tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc aagaatataa
      661 ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat tacaatacat
      721 catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc ctttgacaac
      781 tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta tcgctgcagt
      841 attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca atacaatcag
      901 tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt caaaagatgt
      961 aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg cagcaatcat
     1021 tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta gtgaacgtac
     1081 acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat tgaatccaat
     1141 tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg gtgaacgtca
     1201 accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat cgtacttgct
     1261 attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat tgtggttgtt
     1321 gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg gtttgtctgt
     1381 gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt ggttcttagc
     1441 tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac cgggaacaga
     1501 atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct tgctatttac
     1561 tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga acataaaaac
     1621 aggaattgct tatttgcagc agttcctg
//
LOCUS       DAYYNQ010000197         1644 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000197 DAYYNQ010000000
VERSION     DAYYNQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.205"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..822
                     /locus_tag="ACHBJ1_002952"
     CDS             130..822
                     /locus_tag="ACHBJ1_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9431816.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
     gene            1250..1642
                     /locus_tag="ACHBJ1_002953"
     CDS             1250..1642
                     /locus_tag="ACHBJ1_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431817.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
BASE COUNT          700 a          176 c          249 g          519 t
ORIGIN      
        1 gaattgaatt tctctatcct ttttaaataa ttttttagac aaaaaacaga aagtgtctaa
       61 agaaagcttg acgaagaaaa taatttttaa taacttattt aaggaaattt tttgaaggag
      121 aagataaaag tgagaaaaaa gattattatt aatgctggag taattatagg attgatgtta
      181 gtaattttct ccggaaagca atatgctgat aatattttat tatggggagg ggaagataat
      241 attgaaaaga tcaacaacaa tttaactata ttagacacag ctctagaaaa taaagagcaa
      301 aaaattagta ttttaaattc acaattgtct tcaaacaacc aaactcttga acaactaaga
      361 actaatattg aagtttccaa acaaaaagtg gcatctttag aaaacgacaa gaatcaatta
      421 gtatcagaga aaactaattt agaaaatcaa ttgtcagcaa aagactctga attgcaagga
      481 aaacaaaatg aaattaatgc gaaaatagac gaaattaacc aaaaagtagc tgaaataaat
      541 cgattaaata gcgattggaa taatcgttta caaaatgaaa agaacaaatt taatgaagaa
      601 caatctacta ttaatagttt aaaccagcaa attaatcaaa tgttgagtga aaaaaataac
      661 attcaaaatc aactgcaagc tactcagcaa aagctagaag aagaaaagtc aaaaaatagt
      721 agtttgaata attacattga aaagcttcaa aaagctaaaa atgatgtaga ggataccgca
      781 aataaagcac aacaaattgt aaatgaacat acaatccaat agatatagtt ttaatttaac
      841 aaatggtatg tttggagata atgagataga atataaatgt cataatatta tacaatctaa
      901 acaccgtttc cttttctatt tttgatgaca tcagtatata gctactcgaa agagtagtct
      961 ttttattatt ctcgcttatt attgatatga aaatttcgat ttgacaaaag gtaccgaata
     1021 attttgtaat atgaattatt atcaagaagt aattatgatg ataacatatg gaaactagtt
     1081 agtaataata acaggtgatt aaacttttga acgacttgta attctatatt ttttctttta
     1141 agtaaaaaat ataggtgatt ttttttgact tcatatttat aataaagaca cgtaaatcaa
     1201 ttatttttca ttttacttta actaataatg tttatttgga gggattatta tgttagatga
     1261 tagattgacg tgttctaatt gtggtacatt attttactat gaggaagtta gaaatattgt
     1321 aaaacataga aaaaaagaaa tgcctatacg ttgtccaaat tgcaatgaag ttattaagcg
     1381 aaagttgtct catggttatt ttgtcagcta cgaactatat gaagaaagta aaataaatct
     1441 gaaaaaaaaa ccaaaatact ataaaaattt gaaaactgga gctattataa ctgaaaaaga
     1501 atttaaaaat ttagtgactg aagaagaaag caggttttat aatttgtctg gtaatatttg
     1561 ccctggattt atggatcaga ttattgaaaa tgagtcaatt gaatcaattg aaaactatgt
     1621 tcctgttgac ttaccaaaat agaa
//
LOCUS       DAYYNQ010000198         1639 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000198 DAYYNQ010000000
VERSION     DAYYNQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.206"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACHBJ1_002954"
     CDS             288..695
                     /locus_tag="ACHBJ1_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431818.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACHBJ1_002955"
     CDS             709..1110
                     /locus_tag="ACHBJ1_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431819.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACHBJ1_002956"
     CDS             1112..1483
                     /locus_tag="ACHBJ1_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431820.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          612 a          214 c          283 g          530 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttc
//
LOCUS       DAYYNQ010000199         1613 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000199 DAYYNQ010000000
VERSION     DAYYNQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.207"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1576
                     /locus_tag="ACHBJ1_002957"
     CDS             <1..1576
                     /locus_tag="ACHBJ1_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM9431821.1"
                     /translation="QINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDNAYT
                     KFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDP
                     DFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLAR
                     KRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEK
                     TKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSS
                     MESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIG
                     VYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDARE
                     EIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSN
                     EWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAW
                     RKMLDDELIPLENELR"
BASE COUNT          591 a          264 c          328 g          430 t
ORIGIN      
        1 acagatcaat cgtatgatcg atcaaatgga gtctgtcaaa aaagaaagtt atttggaccc
       61 tactgttttt gaagcagagg aacatatcca aaaagtcatt cataaaatgt tccgtccaga
      121 caatgcctat acaaaatttg attttcgaaa tggccgtcca acgaagtctg cgatcttaac
      181 cacgagttcc attgatatgg ccaaaaagta ttatcgggcc atcaaagaga tgacgaaaga
      241 atctgattgg ttaataaaag agtttcccaa tcagcctatc cgtgaaggac gaacgatgga
      301 ggatcctgat ttcccacgaa tcgctattac ctattcatta gaagaaaata gccgagattc
      361 aagtgcccaa caagaagaaa tgcaaaaaat tattgaggaa tataatcagt attatgatac
      421 tgcctggtca ctagcagaca ttgagcgtta taacggagac atcaataatc gtttggcacg
      481 aaaaagagcg gaattcaaac aatttgggaa acaaatcgat ttagtaatcg tagtggatcg
      541 tctattgaca ggttttgacg cccctacgat tcaaacccta tttgtggatc gtaatctaga
      601 gtatgcagga ttgatccaag ccttttcacg tacgaaccgt acgtatcctg aaaaaacaaa
      661 aggactaatt gttactttcc gaaagccagc gacaatggag aagaatgtcg aggatgcgac
      721 aaaactgtat tcagaagcta aagaagaatc gggtttgatt tatccaagtt accaagaatc
      781 caaaaaaaga ttcaagcaag catatggtaa gttaaaagag gtttcatcca tggaatccat
      841 tgatgaacat acgccacttg aaatccgtat cgagtacgtc aaagcatttc aagaattgaa
      901 tcgtgcgtat gaatcgttag tgacttatga tgattacaat gatgacatgg tgcaatcttc
      961 aactttgaac aatcaaattc aacttttgga agaacaaatt ggcgtgtacg agaccgtgaa
     1021 gggttcattg atggaggaag aaccagaaaa agagggagaa gatttttcaa cgatcgaatt
     1081 ttatagtgaa aataccacta agttgtatga tattgattct acttatattg atcaattact
     1141 aggaacttat gctgcgaata gttcagatgc acgagaagaa attgaaaaag ctttggctaa
     1201 attgaataaa acagaaggag tcaaagaagt ttaccgacaa attttaaatg catttgatgt
     1261 ggggacttta gatagtaatg aagatatctt tgtcattaaa cgtcgctatt ttacacaagc
     1321 aagtgataat ttgattcatg ccttttccaa tgagtggttt gtttctgaga aagaattaca
     1381 tgcttctaat ctgcaatata tgccaggtga agatccaatt cctaatatga aagcaattat
     1441 caacagtaag gattacgaag gatataaagc caaacatcca gaagcgaaac catttaaata
     1501 cccacaagaa atgaagcgag catggcgtaa aatgttagat gatgagttga ttccacttga
     1561 aaatgaattg aggtaaacga gaagagttaa gaaaattgta cgattcaaag ata
//
LOCUS       DAYYNQ010000200         1604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000200 DAYYNQ010000000
VERSION     DAYYNQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.208"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..259
                     /locus_tag="ACHBJ1_002958"
     CDS             <1..259
                     /locus_tag="ACHBJ1_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431822.1"
                     /translation="NNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHDV
                     DRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(621..860)
                     /locus_tag="ACHBJ1_002959"
     CDS             complement(621..860)
                     /locus_tag="ACHBJ1_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431823.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1146..1460
                     /locus_tag="ACHBJ1_002960"
     CDS             1146..1460
                     /locus_tag="ACHBJ1_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431824.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          550 a          219 c          281 g          554 t
ORIGIN      
        1 gaataacgaa actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca
       61 aaaagtgatt ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga
      121 atatcatgac gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata
      181 tactcacgtt tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga
      241 acatacttta gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct
      301 tttgaaggca ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg
      361 ttcgcctaga gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat
      421 actgccagaa ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga
      481 tccaatcatt tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc
      541 gtaaagcatt tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt
      601 tgctgaagaa gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac
      661 agtttcttta ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga
      721 gaatttttct catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa
      781 ggtatgttct ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg
      841 ataaacagaa ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta
      901 tgctcagtat taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc
      961 ttagtaatac atatatggag agggtgatat cccagttcaa accagataca ttggttacca
     1021 tctgtattaa cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac
     1081 gtaggtatga tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat
     1141 agcttttgga tttttacagt aattttatct taataatagc aatattatta cttctaaata
     1201 tttggttttt tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt
     1261 cttctgaaaa aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca
     1321 gtttactttc ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta
     1381 tatctattat tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag
     1441 agaagtcaaa aaataattaa agcaattatg ccttttagta caggaattat tacttcttac
     1501 aaatgtatag cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa
     1561 caattatttt atgttaggat gaattaattt ctctagagtt tata
//
LOCUS       DAYYNQ010000201         1604 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000201 DAYYNQ010000000
VERSION     DAYYNQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.209"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            7..429
                     /locus_tag="ACHBJ1_002961"
     CDS             7..429
                     /locus_tag="ACHBJ1_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431825.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            439..642
                     /locus_tag="ACHBJ1_002962"
     CDS             439..642
                     /locus_tag="ACHBJ1_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431826.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            853..1437
                     /locus_tag="ACHBJ1_002963"
     CDS             853..1437
                     /locus_tag="ACHBJ1_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM9431827.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          571 a          232 c          294 g          507 t
ORIGIN      
        1 tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg aattagtgta
       61 actggaacta caattcctac atttgctgat agtacaaatg aaccaactac tgtagtagat
      121 aataaccaag aattaactcc tttagatagg aacggaatag ccattggtaa cccaactaac
      181 gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctcg agcagcttct
      241 aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc ttctagacct
      301 cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg aaaattgtat
      361 ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg aaccttaact
      421 agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta tcaagatagc
      481 gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt tcagcgaact
      541 ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc tgaagaaaaa
      601 agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta aaaaaagagt
      661 gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc atttctggac
      721 actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca taaacccata
      781 acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa aattaatgaa
      841 ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca aaatttggat
      901 ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga tcatatgagt
      961 ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg ctctggcgat
     1021 acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt gatttcaatc
     1081 gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa tattacgatg
     1141 gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt tgcagaattt
     1201 gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg tgcaagagga
     1261 cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact gaagacttta
     1321 gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc aagaaccacc
     1381 atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc tcattagttt
     1441 ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat agtatgagaa
     1501 ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt tattcatttg
     1561 ttaatattaa agaacggaac tccaaaaaag tccgtactct aatt
//
LOCUS       DAYYNQ010000202         1483 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000202 DAYYNQ010000000
VERSION     DAYYNQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.210"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHBJ1_002964"
     CDS             64..972
                     /locus_tag="ACHBJ1_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9431828.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1483
                     /locus_tag="ACHBJ1_002965"
     CDS             972..>1483
                     /locus_tag="ACHBJ1_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431829.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGV"
BASE COUNT          511 a          216 c          345 g          411 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgt
//
LOCUS       DAYYNQ010000203         1441 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000203 DAYYNQ010000000
VERSION     DAYYNQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.211"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACHBJ1_002966"
     CDS             <1..387
                     /locus_tag="ACHBJ1_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9431830.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            810..1441
                     /locus_tag="ACHBJ1_002967"
                     /pseudo
     CDS             810..1441
                     /locus_tag="ACHBJ1_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          509 a          262 c          251 g          419 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca
      421 caaaattatt gacgctccct gtttcaaagt tagtagagaa aatgtagtta tatatttaaa
      481 tttttaccag aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta
      541 atcatcagga aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct
      601 agcttgcttt tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc
      661 ttctattcaa atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac
      721 cgtagcgcaa ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga
      781 ttcttcagtc ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact
      841 atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt
      901 cgcacgcttc aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac
      961 tcattgatat tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg
     1021 caaatcctga agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga
     1081 caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt
     1141 tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc
     1201 aacgacggga agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa
     1261 aaatcaatca ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac
     1321 tcactgaagc aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct
     1381 atactttttt ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta
     1441 c
//
LOCUS       DAYYNQ010000204         1364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000204 DAYYNQ010000000
VERSION     DAYYNQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.212"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBJ1_002968"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHBJ1_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM9431831.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          393 a          242 c          216 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DAYYNQ010000205         1362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000205 DAYYNQ010000000
VERSION     DAYYNQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.213"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1362
                     /locus_tag="ACHBJ1_002969"
     CDS             82..>1362
                     /locus_tag="ACHBJ1_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM9431832.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAYYNQ010000206         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000206 DAYYNQ010000000
VERSION     DAYYNQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.214"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHBJ1_002970"
     rRNA            <1..1355
                     /locus_tag="ACHBJ1_002970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYNQ010000207         1327 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000207 DAYYNQ010000000
VERSION     DAYYNQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.215"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..201)
                     /locus_tag="ACHBJ1_002971"
     CDS             complement(<1..201)
                     /locus_tag="ACHBJ1_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431833.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSS"
     gene            complement(203..>1327)
                     /locus_tag="ACHBJ1_002972"
     CDS             complement(203..>1327)
                     /locus_tag="ACHBJ1_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM9431834.1"
                     /translation="LITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQ
                     FIFLISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKIT
                     KTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKI
                     KWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQH
                     SSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELK
                     RAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFIL
                     SIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          514 a          224 c          160 g          429 t
ORIGIN      
        1 ggaagaaccg attttccggt gattcacaaa aacaacatca ttaaatagcc ctaattcttt
       61 tatacgctca ctatatcttc tagcgttttc aaattcagaa taaataaata aatctccttt
      121 tgattctcgg taaaaatttt gataaacatt taatatatta atcaaatgta aaggagtcca
      181 agcaatgaaa gctgtcttca tattaattct ccttttttaa atacaagtaa gaaatgcttc
      241 ccaaataata ccagaaaaca attgaaatga catcgcgctt attgaaaagt acatgagcct
      301 ccaccatttc atctataaaa atagatagta taaaaattaa aacaatacca agaaacgcat
      361 aattcacttt acttctaaaa aatgttttga gtaaactaaa gatatttaac acatagaaag
      421 catagataca tataaaagca attataccac ttgatacaag tacttgaaga aatccattat
      481 gcatgtttcc atgtgctctt tttagttcat tcacatctgt agtatcaaga tctacttttt
      541 tcaaaaattt tgcatcgcta ttcccataga aatcggtctc tccataacca aataacaaag
      601 ttggtcttat aactttaagc gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg
      661 agttatcact agaatgctgt aatattactt ctttttctgg aagtggagtt ttatctgatt
      721 catttgactc tataacttct acttttctat caatcgatac aggaattgtt gctaaaaaat
      781 ccttagcata attagtacca aaagaaaaat ataccaatag aactaataat atgcttacca
      841 tccatttgat ttttacaggt agtacttctc taatgctctt tctaaatttt gaattggcaa
      901 acaacaaaag aaataatact aaaaaagaac aaattactac ctgggcacct ctagacgaag
      961 aaagtgtaat ataaaatatt tgtacgataa aattcatcat ataaaaaaca cgaatcgttt
     1021 ttgttatttt tgaaattaat acactaatta aacttaaaat aatagaagta tatccaaaca
     1081 tagctccaac atttggactt gtatagacac caaataaccg atgttcaata aaaccttgtc
     1141 ttgcaagtaa tcctggcgtt cctgttggga catgataaga aatcaaaaaa ataaattgat
     1201 agatagatat tagcgcagct aaaaaaatta tgataatgaa aacattatta aaatacacca
     1261 tttttttctt tttttcctct tcactttgtg aaaagtctgc aggaaaaatt actatcaaag
     1321 taatcag
//
LOCUS       DAYYNQ010000208         1314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000208 DAYYNQ010000000
VERSION     DAYYNQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.216"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHBJ1_002973"
     CDS             <1..276
                     /locus_tag="ACHBJ1_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM9431835.1"
                     /translation="EVTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQ
                     CGIIKMRDYLILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            281..1228
                     /locus_tag="ACHBJ1_002974"
     CDS             281..1228
                     /locus_tag="ACHBJ1_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM9431836.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          435 a          189 c          271 g          419 t
ORIGIN      
        1 gaagttacta tagttaataa atcaagatac acatctaggc aacagacacc tgaagttttt
       61 gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag taattatatt
      121 tttgatgggc aatgtgggat aataaaaatg agagattatc taattttaga tattgatagt
      181 gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag
      241 cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac
      301 cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt
      361 aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat
      421 cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa
      481 taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg
      541 accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg
      601 caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga
      661 tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt
      721 tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg
      781 tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag
      841 tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc
      901 atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat
      961 ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg
     1021 aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt
     1081 agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga
     1141 tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac
     1201 taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc
     1261 aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga taga
//
LOCUS       DAYYNQ010000209         1282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000209 DAYYNQ010000000
VERSION     DAYYNQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.217"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..421)
                     /locus_tag="ACHBJ1_002975"
     CDS             complement(<1..421)
                     /locus_tag="ACHBJ1_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431837.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYL"
     gene            complement(438..>1282)
                     /locus_tag="ACHBJ1_002976"
     CDS             complement(438..>1282)
                     /locus_tag="ACHBJ1_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9431838.1"
                     /translation="YSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIK
                     IDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEK
                     NYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKE
                     IGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYN
                     YISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNF
                     NNIS"
BASE COUNT          442 a          221 c          140 g          479 t
ORIGIN      
        1 tcaaatactt ataatctatt aacttgtagt tgtttaactt ttctgaaaaa gttagaccca
       61 cgtaataatc ttcccagctt tctctttcaa ttaactgatc caatttttga taatccataa
      121 attcatcatg tatcaaaaag aataaataac ttgatatttt agcatctagc ttaatcagat
      181 taaataatgt ttgtttaaaa ttgctaccat taatatgact gatagattct ctagcttctt
      241 cacataaact ttttacttta ttaattacta ctttgtcata ttcatttaac acactattat
      301 taccattact tagacgctca gattctacga aacaactcaa atcagaaatt tcccaaaaaa
      361 attttttgtt aaaatccaat ttcctcaact cctccaaact acgtaaatga tatagtctca
      421 taataaaata aaacctctca gctaatgttg ttgaaattgt ccactaaacc taattgatat
      481 tgcttaggag atacacctgc gtactttttg aatactctcg aaaagtgtga agaatctgaa
      541 aatcctaaac ctaaagaaac ttcactgata gtatggtttg atttcaacat tattttagcc
      601 gtagaaattt ttcgcttaga gatataatta taaatcgtaa tatgcatttc attcttaaag
      661 ctttctctta atttagtttc acttacagaa aaataattag ctatttcctt tacagtaatt
      721 cttttataga tatttaagcc gatgaattga acgacagaag aaattaaggg agaaagatgc
      781 tctatcttag tttttccgat ttccttagta taataaacaa tcccagaatc cctcactttt
      841 aagacctcag gtaacgatac agctaattcg ttttcttgta tcaaagcatc tctactttga
      901 tacgcttcta ttatatccat tcctaatgtt atagacgttt gtgctaactt ttcaaaaaca
      961 atgatagaat aatttttttc agaccgaatc gtatctccac taacacttgg aattattcca
     1021 ttactcagat taaaaaccat gttttctaaa tttcgaatat ctccattttt aacatattcc
     1081 agtattttat tttcataaag aaacagatat atttctggat cgtaattttg tgataacaga
     1141 ttatctattt tgatttgctc gatagtttta cttagatttt ctgtatattc aattaacggt
     1201 tcatggaata gatcttctat ttttccagta aaaaaataat gaactaaaat cagaatatct
     1261 cgaatgtctc ctaaagaata ca
//
LOCUS       DAYYNQ010000210         1260 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000210 DAYYNQ010000000
VERSION     DAYYNQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.218"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHBJ1_002977"
     CDS             complement(93..1136)
                     /locus_tag="ACHBJ1_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM9431839.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          433 a          251 c          238 g          338 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
//
LOCUS       DAYYNQ010000211         1251 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000211 DAYYNQ010000000
VERSION     DAYYNQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.219"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>1251
                     /locus_tag="ACHBJ1_002978"
     CDS             56..>1251
                     /locus_tag="ACHBJ1_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9431840.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNN"
BASE COUNT          385 a          250 c          245 g          371 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc a
//
LOCUS       DAYYNQ010000212         1215 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000212 DAYYNQ010000000
VERSION     DAYYNQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.220"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACHBJ1_002979"
     CDS             414..947
                     /locus_tag="ACHBJ1_002979"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM9431841.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAYYNQ010000213         1212 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000213 DAYYNQ010000000
VERSION     DAYYNQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.221"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..969
                     /gene="scrK"
                     /locus_tag="ACHBJ1_002980"
     CDS             97..969
                     /gene="scrK"
                     /locus_tag="ACHBJ1_002980"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM9431842.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          360 a          224 c          284 g          344 t
ORIGIN      
        1 atttacttgt catgttacta aataaaaaaa taatttaaat atgatatata gtcatttatt
       61 tagtgatttc attctaaaat aaagggagaa atgtctatgc tatacggtgc aattgaagca
      121 ggcggcacaa aatttgtgtg tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg
      181 agctttccga caacgacacc tgaggaaaca atgcctttag tcattgactt cttcaaacaa
      241 taccaagcag atttagcagg gattgggatt ggctcatttg ggcctatcga tatccatcgc
      301 gattccgcca cttatgggta cattacctcg accccgaaat tggcatggca aaattttgat
      361 ttcatcggta ccatgaaaaa agaatttccg atcccgattt cttggacaac agatgtcaat
      421 gcggctgcct atggtgagta tgtcttcggc agtggcaaag gattgagcag tgtcgtttac
      481 tatacgatcg gtacgggtgt tggcggcggt gccttacaag aagggcgctt tatcgaaggg
      541 tttagtcatc cagaaatggg gcatatgctc gtcgtccctc atccaaaaga tgactttgct
      601 ggcagctgtc catttcatgg caactgttta gaagggatgg ccgctggacc cgccatcgaa
      661 aaccgcttag ggaaaaaagg acaagaggtt gcagaagatg atccttattg ggaaatcgaa
      721 gcctcctata ttgctcaatg tgcctacaat acaaccttga tgttttctcc agatgtgatc
      781 atttttggcg gtggtgtgat gaaacaacgc catatgctgc aaaacgtcca cgacgcattt
      841 gcaaaattgg tcaatgggta tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca
      901 gcattagaag acaacgctgg aacgctcggt tgtttggcat tggctcgaga agcggtctta
      961 cattcataaa cgaaaaaagg ctcttcgaca tttagtgttg gtagagaaag atacaactga
     1021 aaatttagtc ataaccagtt aggcagagtg cttaactggt ttttgtttta cttggaagat
     1081 caagccttga atgagataaa ttcttgctcg aaagttatgg aaatttcgat agccgtaggc
     1141 cacttgtatt gtgcaatagt tttttacaaa aaaaaggggg tgcggatatt tttttggacc
     1201 tttttaacaa at
//
LOCUS       DAYYNQ010000214         1205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000214 DAYYNQ010000000
VERSION     DAYYNQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.222"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1101
                     /locus_tag="ACHBJ1_002981"
     CDS             <1..1101
                     /locus_tag="ACHBJ1_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9431843.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT          397 a          244 c          227 g          337 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgagg
//
LOCUS       DAYYNQ010000215         1195 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000215 DAYYNQ010000000
VERSION     DAYYNQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.223"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACHBJ1_002982"
     CDS             <1..1076
                     /locus_tag="ACHBJ1_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9431844.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAYYNQ010000216         1187 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000216 DAYYNQ010000000
VERSION     DAYYNQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.224"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1002
                     /locus_tag="ACHBJ1_002983"
     CDS             <1..1002
                     /locus_tag="ACHBJ1_002983"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM9431845.1"
                     /translation="ILIRVRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAV
                     LMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAR
                     EFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSK
                     KWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAAC
                     WFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNK
                     LMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1023..>1187
                     /locus_tag="ACHBJ1_002984"
     CDS             1023..>1187
                     /locus_tag="ACHBJ1_002984"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFM9431846.1"
                     /translation="MANQLFHIVITTHSTLCEGYLKATKLILMNDATGISAVPFKENT
                     SPDDFEESMKE"
BASE COUNT          420 a          158 c          234 g          375 t
ORIGIN      
        1 atacttatta gagtgaggga taaagaaatg agacaactac acaaagaaca gattgaaaca
       61 gcaatggcag cgttaaagga aaagacaatt gatcatgtat attttacagc atgtgggggt
      121 tcacaagccg ttttaatggc tggtcaatat ctatttgata aagaattaac tgtaccttgt
      181 catgtttata ctgctaacga atttaactat gctataccaa aaggattatc tgaaaattct
      241 ttagttatca cttgttccca ttctggcaca acaccagaaa ctgtagaagc aacaaaaaaa
      301 gcaagagaat ttggagcaat gactatttgt ttatctaatg aagaaggatc tcctctatgg
      361 gaagcagctg aatatcctgt tcactatgat tggggtaaag atgttgatgc tagtgattta
      421 aataaaggaa ttctttatgg tttattattt aatattttac ttgttttaga tggctctaaa
      481 aaatgggaaa cttgcttgaa tgaatttgat aagttaaatg atcttgttat tgctgaaaag
      541 agcaaatttg aaataactgc taaagaatgg ggtaaaaacc aaaaacgaga taaaattatt
      601 tacacaattg gtagcggtat caattacgga gaaatttact ccacagcagc ttgctggttc
      661 atggaaatgc aatggattaa ttctaatgct attcattctg gggaattttt ccatggtcct
      721 tttgaaatca ctgattatga tgtaccattc atcttagtga aaagtattgg agatacaaga
      781 tttttagatc aacgggtaga agacttcact aaaaaattca gtaataaatt aatggtttta
      841 gaccaagctg attttgactt agaatctata gatagtgtag cgaaagaata tattgcagct
      901 gtacttagtg gcgttgttat tcgccaaatg gtagaagcga ttgcttttga aagaggccat
      961 tccttagatg ttagacgcta tatgtggcaa atgaaatatt aattaatagg aggttgcaaa
     1021 agatggcaaa tcaattattt cacattgtga ttacaacaca ttctaccctt tgtgagggtt
     1081 atttaaaggc aacaaaatta attttaatga atgacgccac aggtattagt gctgttccat
     1141 ttaaagaaaa tactagtcca gatgattttg aggagagtat gaaagaa
//
LOCUS       DAYYNQ010000217         1161 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000217 DAYYNQ010000000
VERSION     DAYYNQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.225"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..776
                     /locus_tag="ACHBJ1_002985"
     CDS             <1..776
                     /locus_tag="ACHBJ1_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM9431847.1"
                     /translation="DSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYL
                     VKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANAD
                     VEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMT
                     RRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFF
                     KVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            848..>1161
                     /locus_tag="ACHBJ1_002986"
     CDS             848..>1161
                     /locus_tag="ACHBJ1_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431848.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLV"
BASE COUNT          509 a          134 c          194 g          324 t
ORIGIN      
        1 tagactcgac tagggaaaaa atcataaaaa tatttgataa aattaataat ttattcaaga
       61 atgacaatgc tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc
      121 attactctta tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac
      181 agatgataga agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata
      241 aagaacaacg tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta
      301 atgcagatgt ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa
      361 aagaattaga agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt
      421 atgtgtatgt aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga
      481 ctagaagatt agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg
      541 aatttgacgt tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc
      601 ataatcattt tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag
      661 tttcactaga cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta
      721 caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat
      781 ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat
      841 agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg aacgttatac
      901 ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca attcacgcca
      961 agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa
     1021 tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc
     1081 aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa tttcaaaata
     1141 tataaaagat gatgtacttg t
//
LOCUS       DAYYNQ010000218         1135 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000218 DAYYNQ010000000
VERSION     DAYYNQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.226"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..581)
                     /locus_tag="ACHBJ1_002987"
     CDS             complement(51..581)
                     /locus_tag="ACHBJ1_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM9431849.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(680..1030)
                     /locus_tag="ACHBJ1_002988"
     CDS             complement(680..1030)
                     /locus_tag="ACHBJ1_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM9431850.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT          410 a          205 c          181 g          339 t
ORIGIN      
        1 ttttttggat tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata
       61 gtcatttttc ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata
      121 aagccgaata tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat
      181 ccgccaatca acaacagaat actactataa acactccaac ttttagcttt tgtattcatg
      241 ctgaagaaca actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg
      301 atagaaacga ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga
      361 ccactgaaca gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa
      421 agattcaaca acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa
      481 acagcagaag ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat
      541 aaggcgtatt tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga
      601 cttattttaa cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag
      661 aagaatatct ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta
      721 gctcatcgat cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt
      781 ctactcgtac accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg
      841 cttccgcaaa aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt
      901 gggaataacc cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc
      961 tctccgcttt tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat
     1021 tttgcgccat aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa
     1081 gaaaaagaga agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaat
//
LOCUS       DAYYNQ010000219         1108 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000219 DAYYNQ010000000
VERSION     DAYYNQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.227"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..733
                     /locus_tag="ACHBJ1_002989"
     CDS             167..733
                     /locus_tag="ACHBJ1_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9431851.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            878..965
                     /locus_tag="ACHBJ1_002990"
     tRNA            878..965
                     /locus_tag="ACHBJ1_002990"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:910..912,aa:Ser,seq:cga)
BASE COUNT          392 a          168 c          221 g          327 t
ORIGIN      
        1 ggaacccatt gaatttaacc aaattttttt aattttgcaa aaaaaagtta aagaagactg
       61 aaaaaaattt aaaatataac gttttgagta aaatgtttgc aaaaaatgct aaaaataggg
      121 tacaataaaa caacatatac tgcagtaaag aagtggggga gttttaatga gaagaaaagt
      181 ctataccaag gatcaaattc taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa
      241 aggatttact gcgagaaata ttgctaaaaa gatggggatt tcgacacagc cgatttatct
      301 ggaattcaaa aacatggaag atttaaaaaa tacgttgctg gattctatct ttgaacaact
      361 aaaagaagat gttttcccta ttgaacgtac tggtgataaa atcattgatc tcggtttgaa
      421 ctatatctac tttgcaaaaa agcatcatag tctttacatg gcattgtaca ttgatgaaca
      481 tggggacgga aagaaaatgc atgatttttc atacaattat ttccgtgaaa tagttaaaga
      541 agatgaaaga tacaatcaat tatcggatga atatgtcgat gctctgcata ctggtacatg
      601 ggtagcagtg actggcttag cgtcattgat gtcatcaaac atcatgcatc caacagaaga
      661 acaaatcatt gcactgatca aacggacaat tgactcgatt ttagcattag ataatcctga
      721 aataatcaga tagtgttcta tcagtatgaa tttgatcggt aagtccgttg gctgcaaact
      781 gtcaatggac ttttttgcta aaaaaagcga tttctgttat ttatctatta gaaataggct
      841 tgacatttcg tgaaaaaagt ttaaaaatag tacttacgga aggttggcag agtggtaatg
      901 caccggactc gaaatccggc gaaccggcta ataccggcgc gcaggttcaa atcctgtacc
      961 ttccttatat caacgattag aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg
     1021 gccaaacaaa aatagctttt cctatttttt cgtttggttg ggcttattac cgcagatgca
     1081 agcttttgaa caccgtttgt tcggaata
//
LOCUS       DAYYNQ010000220         1097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000220 DAYYNQ010000000
VERSION     DAYYNQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.228"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACHBJ1_002991"
     CDS             complement(<1..229)
                     /locus_tag="ACHBJ1_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM9431852.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFK"
     gene            923..>1097
                     /locus_tag="ACHBJ1_002992"
     CDS             923..>1097
                     /locus_tag="ACHBJ1_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM9431853.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQP"
BASE COUNT          410 a          173 c          133 g          381 t
ORIGIN      
        1 ttttaaacat gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt
       61 tagttttttt aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc
      121 tatctgagcg atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact
      181 ttaaacaaac atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc
      241 tctttccttt ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg
      301 tcaaaataag ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc
      361 accgtaaaaa gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat
      421 ttacatatat ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata
      481 cagataatag gaatttttcg tttctaaata ttccaatttg ggaatccaat aaaaattttc
      541 gttcaaatac gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat
      601 aaaaaataca atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt
      661 tattatacgt aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag
      721 aagatatatg aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa
      781 catttttgaa aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc
      841 gcagcgcagc ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat
      901 cttaatggag gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat
      961 tcgattcttt gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga
     1021 aattcaaaaa catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat
     1081 aaaatttaaa caaccga
//
LOCUS       DAYYNQ010000221         1076 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000221 DAYYNQ010000000
VERSION     DAYYNQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.229"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..568
                     /locus_tag="ACHBJ1_002993"
     CDS             <1..568
                     /locus_tag="ACHBJ1_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM9431854.1"
                     /translation="INAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERK
                     EEFDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQG
                     VRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEF
                     PIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            568..>1076
                     /locus_tag="ACHBJ1_002994"
     CDS             568..>1076
                     /locus_tag="ACHBJ1_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431855.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          381 a          139 c          240 g          316 t
ORIGIN      
        1 catcaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caattgaaga
       61 tttaattgct gatatgacag attttattaa tgaaaataaa ggcgatttta cggactggat
      121 gaatgaaagg aaagaagaat ttgatcgctg gagagaggaa caggagaata gtttccaaga
      181 ttggagagaa ggacaagaat cagattactt gtcatggttt gaatcaatca aggatatttt
      241 gaagtctatc gatccaggtg gagtaatgtt agccgaatta atggatgcac gtgtagatct
      301 tcaaggtgta cgccatgaat cgatttctga acgtttccta tccgatctaa attatttgta
      361 tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt gaaattgaag tgactgatat
      421 tcttcaagac gatctctttt cagataatca cgaagtcgaa aaaattgaaa cagtagaatt
      481 tccaattgaa gaaggagctt tgattattgc aactgttgat gatccaaaac agaatgtttt
      541 cactcttgag aaagttgggg tgatttgatg gttaaagtaa agagaatgat ggaaactgat
      601 gaatctggtg ttaaacgtca atttatgcca attacacatg tctctgcagt tttaggctta
      661 gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta atggtaaata tggtgcagtc
      721 attttaacga aagctgattt aggattagag aatgcaatta ctgaactacc ttatgcaagc
      781 gagacaagcg atggtattct tactgctgaa atgtttcaaa agatcgtaaa tggagaaggt
      841 ggaacatata ttttgccgat tgctactcct gaacaattag gaggtataaa agttggggaa
      901 ctcttagaag ttactgaaga aggtgtttta tctgcaacta agcaaacaga cttcaatttc
      961 tctgaagaat taaaaggaaa attagaatct ctaaaaatat taaaagcagg tgcaaatata
     1021 tcaattgcag aagatggaac aattagttct acaggtggta gtgggactgg tggtgt
//
LOCUS       DAYYNQ010000222         1069 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000222 DAYYNQ010000000
VERSION     DAYYNQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.230"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACHBJ1_002995"
     CDS             <1..969
                     /locus_tag="ACHBJ1_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM9431856.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DAYYNQ010000223         1064 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000223 DAYYNQ010000000
VERSION     DAYYNQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.232"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACHBJ1_002996"
     CDS             complement(37..993)
                     /locus_tag="ACHBJ1_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431857.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          380 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aact
//
LOCUS       DAYYNQ010000224         1038 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000224 DAYYNQ010000000
VERSION     DAYYNQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.233"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..>1038
                     /locus_tag="ACHBJ1_002997"
     CDS             120..>1038
                     /locus_tag="ACHBJ1_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431858.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSK"
BASE COUNT          355 a          127 c          139 g          417 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt taaaaaaaat aaatgatact atatgtaata taaaaaaata
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctatgact atggtaatga
      721 ttataattgc tttaattatt caattttatc ggaaaaagaa aatagaaatc ttcttaatta
      781 ttaaattaat aaaagctgct atagttactt tacttctttc actagcgttt ataggtccta
      841 tgattgagca gacattgtat caagatctga atagacctta tgtggatata ttacaaaaaa
      901 gagcaattta tctgggtaaa gagtttttga ttggaagtat agattcagaa ttactatcat
      961 ttggtattgg cttaactttg cttatttgtt taattatact aacatttaac tttaaaaaat
     1021 taggaatctt atctaaaa
//
LOCUS       DAYYNQ010000225         1020 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000225 DAYYNQ010000000
VERSION     DAYYNQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.234"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACHBJ1_002998"
     CDS             <1..459
                     /locus_tag="ACHBJ1_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFM9431859.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            468..695
                     /locus_tag="ACHBJ1_002999"
     CDS             468..695
                     /locus_tag="ACHBJ1_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431860.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          318 a          166 c          180 g          356 t
ORIGIN      
        1 ttgctattaa aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt
       61 ttttatactc agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta
      121 tttgtttctg tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg
      181 aaactattga aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta
      241 aaaaaaatcg aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa
      301 tttatgaaag tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc
      361 gtattcattc cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat
      421 gcgattttac ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg
      481 ttaatttaga tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag
      541 tgggcattac gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt
      601 tttccactct cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct
      661 ataaaaatga ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata
      721 aaaaataatc tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca
      781 gattttttat ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac
      841 aaagggggcc ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt
      901 acgttgtagt tggatctgct tttagtataa tactttattc attccaccag actgatagca
      961 gtttgagcag tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt
//
LOCUS       DAYYNQ010000226          998 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000226 DAYYNQ010000000
VERSION     DAYYNQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.235"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHBJ1_003000"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACHBJ1_003000"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM9431861.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          262 a          221 c          157 g          358 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcg
//
LOCUS       DAYYNQ010000227          991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000227 DAYYNQ010000000
VERSION     DAYYNQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.236"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..94)
                     /locus_tag="ACHBJ1_003001"
     tRNA            complement(21..94)
                     /locus_tag="ACHBJ1_003001"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ACHBJ1_003002"
     tRNA            complement(108..181)
                     /locus_tag="ACHBJ1_003002"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ACHBJ1_003003"
     tRNA            complement(209..281)
                     /locus_tag="ACHBJ1_003003"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ACHBJ1_003004"
     tRNA            complement(301..374)
                     /locus_tag="ACHBJ1_003004"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="ACHBJ1_003005"
     tRNA            complement(384..457)
                     /locus_tag="ACHBJ1_003005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="ACHBJ1_003006"
     tRNA            complement(468..553)
                     /locus_tag="ACHBJ1_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="ACHBJ1_003007"
     tRNA            complement(570..641)
                     /locus_tag="ACHBJ1_003007"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="ACHBJ1_003008"
     tRNA            complement(653..725)
                     /locus_tag="ACHBJ1_003008"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="ACHBJ1_003009"
     tRNA            complement(743..824)
                     /locus_tag="ACHBJ1_003009"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="ACHBJ1_003010"
     tRNA            complement(832..904)
                     /locus_tag="ACHBJ1_003010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="ACHBJ1_003011"
     tRNA            complement(908..980)
                     /locus_tag="ACHBJ1_003011"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DAYYNQ010000228          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000228 DAYYNQ010000000
VERSION     DAYYNQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.237"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHBJ1_003012"
     CDS             <1..>986
                     /locus_tag="ACHBJ1_003012"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM9431862.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYNQ010000229          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000229 DAYYNQ010000000
VERSION     DAYYNQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.238"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHBJ1_003013"
     rRNA            <1..>943
                     /locus_tag="ACHBJ1_003013"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYNQ010000230          916 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000230 DAYYNQ010000000
VERSION     DAYYNQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.239"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>916
                     /gene="bsh"
                     /locus_tag="ACHBJ1_003014"
     CDS             4..>916
                     /gene="bsh"
                     /locus_tag="ACHBJ1_003014"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM9431863.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDL"
BASE COUNT          315 a          124 c          166 g          311 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttag
//
LOCUS       DAYYNQ010000231          843 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000231 DAYYNQ010000000
VERSION     DAYYNQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.240"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>843)
                     /locus_tag="ACHBJ1_003015"
     rRNA            complement(<1..>843)
                     /locus_tag="ACHBJ1_003015"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DAYYNQ010000232          701 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000232 DAYYNQ010000000
VERSION     DAYYNQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.241"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..657
                     /locus_tag="ACHBJ1_003016"
     CDS             <1..657
                     /locus_tag="ACHBJ1_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM9431864.1"
                     /translation="NKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYK
                     ERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQ
                     KKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYG
                     NNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS
                     "
BASE COUNT          313 a           47 c           98 g          243 t
ORIGIN      
        1 aataaagaaa ttttaaaatt agaaaagtta aaatatgact tttttaaagg tgcatcccat
       61 gaacttaaaa cccctcttgc tagtcttaaa ataatactgg aaaacatgaa atacaatatt
      121 ggaaaatata aagaaagaga tatttatatt aatgaatgta ttgaaatcgt tgatagttta
      181 acaaaaaata tttctcaaat attatcagtt cattctatag aaaatttgaa taatgatgaa
      241 gaatatttga aaataaatga tacattagaa gacatattaa aaaagtatga aatattagca
      301 aatcaaaaga aaataactgt caataattat atattagacg agaatgttta tataggaaaa
      361 acagctttaa agattattct atctaatttg attagtaatg cggtaaaata tactgatgta
      421 aatggggtaa ttaatattgg gatagttaat gattggttat atatagagaa ttcatacggt
      481 aacaataaaa tttcgaatat ggataaaata tttgatgtta aatttgattt aaataaggaa
      541 aatagtaatg gattaggttt atatattgta agtaatattc tgaataatta caatatagaa
      601 tataaagtat tgcaggatga agaatttttt atctttaaaa taaaaatatt ttcttaatta
      661 agatataatt ttgcaatagt tattgaaaaa tagagactta t
//
LOCUS       DAYYNQ010000233          696 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000233 DAYYNQ010000000
VERSION     DAYYNQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.242"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..629)
                     /locus_tag="ACHBJ1_003017"
     CDS             complement(54..629)
                     /locus_tag="ACHBJ1_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM9431865.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DAYYNQ010000234          663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000234 DAYYNQ010000000
VERSION     DAYYNQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.243"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..346)
                     /locus_tag="ACHBJ1_003018"
     CDS             complement(<1..346)
                     /locus_tag="ACHBJ1_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM9431866.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENF"
BASE COUNT          203 a          118 c           85 g          257 t
ORIGIN      
        1 caaaattttc tactgagata tatttttcct gaaagaaagc caataccatt ttcatagata
       61 aagaatccgt tatataacga gataacagag ctttgctaga acattttcca ttaatttcca
      121 gacatatatc caattcactg acggttactc gcaaattttt ctctagatcg aacttttgta
      181 aatcatgttt cagcttatcg atcgtttttt tcaagagaaa tgtcgatagc gacaactctt
      241 cttgaacatt cgatattgac tctgataaat ttgagcttgc ttctaaatat ttaagtaatg
      301 aataacttct gtgatcttct ttgcttaaaa aaaactcata attcattagt catcagctct
      361 ttctacgtat attactttcc atgcattcac caactagctg gggataaaac tgatttcctg
      421 gatccgagtc actgttgtca gcgattcttg tttgtataat atacatcgtc ctgtcttttt
      481 tttaacattt tggagagcgt tttatttggc ctttctcgcg atcaaacctg ttattagaat
      541 tatatcattt actagtatgc atttttatta ataaaatggt atattttata ttgttataaa
      601 aagagcccaa ccatcctaca ataataaaaa tttactattt gttatcttaa ccttactttt
      661 ctt
//
LOCUS       DAYYNQ010000235          617 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000235 DAYYNQ010000000
VERSION     DAYYNQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.244"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..514)
                     /locus_tag="ACHBJ1_003019"
     CDS             complement(<1..514)
                     /locus_tag="ACHBJ1_003019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431867.1"
                     /translation="MYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSLVITECDYF
                     KKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLEAI
                     NELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVILDK
                     CHFGLNLFKSN"
BASE COUNT          205 a          101 c           69 g          242 t
ORIGIN      
        1 tatttgactt aaataaattt agtccaaaat gacatttatc gagtataact tttttttctg
       61 cattgtcata tataacaccg cagtcaacaa attcaatatg atttttttca caaatatcag
      121 ctaatttagt tttgctttca ccttttccaa taaagtaaag atttattttg tttccaaatt
      181 ttcttttgca tgttttcaat aattcattaa tcgcttctaa atctacgata ttattaatcg
      241 tacctaaata agcaaggttc aacgtagact ctaagctttt attgactatt ttctctccat
      301 aatcattttg gtcatctaca ttgaaagaac atggataaat ggtcatattt tttgattctc
      361 cggaaatttt tatgaatttt ttgaaataat cgcattctgt tataactaga ctagaattac
      421 tgagatattg atctcgtagc tttttccata aatcagtaaa aaatttatat ttgttcccta
      481 ttcccggcat tgactctggc cataaatcat acacatcaaa aataactcga ttgcatctac
      541 taaacttagc aataaaaaaa actaaggtat taggtggtat tctacaataa ataatttcag
      601 gcttttcttt tcgaata
//
LOCUS       DAYYNQ010000236          614 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000236 DAYYNQ010000000
VERSION     DAYYNQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.245"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..464)
                     /locus_tag="ACHBJ1_003020"
     CDS             complement(165..464)
                     /locus_tag="ACHBJ1_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM9431868.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          198 a          111 c           73 g          232 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa cgtctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 aaaaacattc taaa
//
LOCUS       DAYYNQ010000237          598 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000237 DAYYNQ010000000
VERSION     DAYYNQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.246"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>598
                     /locus_tag="ACHBJ1_003021"
     CDS             <1..>598
                     /locus_tag="ACHBJ1_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM9431869.1"
                     /translation="AYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKV
                     GQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSL
                     KEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYP
                     YTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          208 a          120 c          110 g          160 t
ORIGIN      
        1 cgcctacttc cagcttacca aacgtattct gccaaatgcg aaagtagtga ttgatcggtt
       61 tcatattatc aaacacatga atcaagcctt taatgagttg cgtatccgtg aaatgaatga
      121 acttcgtaaa gtaggacaga aaagtcaggc agaaaaactg aaaaagaact ggcgcttttt
      181 gctaaaaaat cgtgcaaaca tcaaccatta tgaatacaaa acatggaaaa gttttcgagc
      241 accaaaatac ccatttctta cggaagcgat gatgattgat cgattgcttg gcttttctac
      301 gtccctgaag gaggcgtatc cctattttca tgaattagtt gaagcctttc gagacaaaga
      361 ccctgactta tttttctcct tattggcaga acttcccgaa acgttagatg acggctttcg
      421 ggaaaagctc caaaaccttc tgacctatga agaaggcatc accaacgcga tgatctatcc
      481 ttacaccaat ggaaaaatag aagcgaagaa tacccatata aagacaatga aacgagtatc
      541 ctacggattt aaatcatttg agaacatgag aattagaatc tttttgatca atcaatta
//
LOCUS       DAYYNQ010000238          591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000238 DAYYNQ010000000
VERSION     DAYYNQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.247"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..216
                     /locus_tag="ACHBJ1_003022"
     CDS             <1..216
                     /locus_tag="ACHBJ1_003022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431870.1"
                     /translation="FRINLPNKFIGGFKMSHTLLIKDTLNILDLNINIHFEENCLTKE
                     KYKGQICLIYKGTLQYTPEEYPHLPIY"
     gene            276..509
                     /locus_tag="ACHBJ1_003023"
                     /pseudo
     CDS             276..509
                     /locus_tag="ACHBJ1_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          222 a          115 c           90 g          164 t
ORIGIN      
        1 tttagaatta atttgcccaa caaattcata ggaggattca agatgtctca tactctgctt
       61 atcaaagata ccctaaatat tctagaccta aatatcaata tccatttcga ggaaaattgt
      121 ttaactaaag aaaaatacaa aggacaaatt tgcttgattt acaaagggac tcttcagtac
      181 acaccagaag aatatccaca tcttccaata tattaaaaga accttcttga atcattgagt
      241 gaaagaaaag agattcgtca ctatttgagg actgtttaac tagagacatt agcgatctgc
      301 ctgaaaccta tacaaccact ttacgaacgt ttaaaaattt tctgccacag attcaaaatg
      361 cgcttaactg cagctattca aacagaccat tggaatgttt aaacaaccac atcaaagtat
      421 taaaacgcaa tacgtatggc tttcgcagct tttacaattt caaactacgg atcatgattc
      481 gtcatggcaa aacattttta accaaataaa cagaagaatg tgggataaat ctaaaaatca
      541 gttttgctac agactctcga gtaaacggcg ggagcagaag caactccttc g
//
LOCUS       DAYYNQ010000239          534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000239 DAYYNQ010000000
VERSION     DAYYNQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.248"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>534
                     /locus_tag="ACHBJ1_003024"
     CDS             <1..>534
                     /locus_tag="ACHBJ1_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM9431871.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DAYYNQ010000240          531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000240 DAYYNQ010000000
VERSION     DAYYNQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.249"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..531
                     /locus_tag="ACHBJ1_003025"
                     /pseudo
     CDS             1..531
                     /locus_tag="ACHBJ1_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
BASE COUNT          193 a          110 c          109 g          119 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata tttctcgtca gacctactat tatcaagcga aaccgatcga
      361 aaatgagtcc gacttagaag agatcgttca ggaagagttt attcgaaacc gaaaggctta
      421 cggtacccga aaattgaaga agtgtttagc aaagcgtggg cttcaactca gtcggcgccg
      481 aatcggtcga atcatgaaac gccgcggatt gacatctacc tatacgatcg c
//
LOCUS       DAYYNQ010000241          522 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000241 DAYYNQ010000000
VERSION     DAYYNQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.250"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          177 a           98 c           58 g          189 t
ORIGIN      
        1 tttttaatat ataacacagt tatttttaaa aataagaatg ctaaatcact ctacaataac
       61 ggcgaaagca caaaaaataa aaaaattatt acgttattaa aaaaacagat gaaagatcag
      121 gttttattag atctcattct ttcatcactt gaagggattt aatgaactac tttttcctct
      181 ttgtcgtttt tatatattcg tattccttca aacagtagga tagcctcttt caatgcttga
      241 tatttgaact gtccgttact taaaaattta gatgtgccgc cctcttccta gaacatattc
      301 ttttttactt tttcattaat tgacagacca atcgaacgaa actgtaaatt tcgataggat
      361 aatgattggt caaactcatt cattttcaag acatctcgat tcatattcaa gataggaatc
      421 tttttatcaa catcacttta ttcgtatttt cctctaccat aaaagcttca cgctccacaa
      481 aattgtttta tacactacta ttcagcccat tcaacgacca at
//
LOCUS       DAYYNQ010000242          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000242 DAYYNQ010000000
VERSION     DAYYNQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.251"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHBJ1_003026"
     CDS             complement(<1..132)
                     /locus_tag="ACHBJ1_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM9431872.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYNQ010000243          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000243 DAYYNQ010000000
VERSION     DAYYNQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.252"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHBJ1_003027"
     CDS             <1..>468
                     /locus_tag="ACHBJ1_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM9431873.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYNQ010000244          460 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000244 DAYYNQ010000000
VERSION     DAYYNQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.253"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHBJ1_003028"
     tRNA            complement(20..91)
                     /locus_tag="ACHBJ1_003028"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHBJ1_003029"
     tRNA            complement(113..200)
                     /locus_tag="ACHBJ1_003029"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHBJ1_003030"
     tRNA            complement(225..298)
                     /locus_tag="ACHBJ1_003030"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHBJ1_003031"
     tRNA            complement(312..382)
                     /locus_tag="ACHBJ1_003031"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHBJ1_003032"
     tRNA            complement(384..459)
                     /locus_tag="ACHBJ1_003032"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAYYNQ010000245          457 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000245 DAYYNQ010000000
VERSION     DAYYNQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.254"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>457
                     /locus_tag="ACHBJ1_003033"
     CDS             <1..>457
                     /locus_tag="ACHBJ1_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM9431874.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          185 a           68 c          106 g           98 t
ORIGIN      
        1 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
       61 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAYYNQ010000246          452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000246 DAYYNQ010000000
VERSION     DAYYNQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.255"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          153 a           55 c           66 g          178 t
ORIGIN      
        1 tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac aaaaaggcaa
       61 agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt aatgttttta
      121 acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag tgtatattat
      181 gctatccttg tttggatttt aggacgttta aagaggcact tttttacata acttaatcca
      241 tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg caccccaaac
      301 cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca ctaagaagca
      361 taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta gagtaattaa
      421 ttagatgttt attttatagg actgatacta ta
//
LOCUS       DAYYNQ010000247          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000247 DAYYNQ010000000
VERSION     DAYYNQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.256"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHBJ1_003034"
     CDS             <1..>451
                     /locus_tag="ACHBJ1_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9431875.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DAYYNQ010000248          377 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000248 DAYYNQ010000000
VERSION     DAYYNQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.259"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..>377
                     /locus_tag="ACHBJ1_003035"
     CDS             39..>377
                     /locus_tag="ACHBJ1_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFM9431876.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYN"
BASE COUNT          151 a           44 c           55 g          127 t
ORIGIN      
        1 aatcctatta taatattttt gttaatgatt ttaataaaat gattggagag aaaatgatgg
       61 atcaaaaagc gtttatcgaa tctattgatt ctacaatcga tagattaaaa aaagaaattc
      121 gattttataa tagagtaatt ggtatcggaa atattataaa aatagtctta tcagctagta
      181 tcccaatatt aattgatcag gcttccgaac acaggtcttt actattgatt gtttcaattg
      241 cttcagcaat aattacaatt atacaaagtg ggatgtctgc atttaattat caagataaag
      301 tacagacttc gacagagtta ttaatgaaaa ttgaaaaaga aaagttgtta tatataacga
      361 aaacatcccc atataat
//
LOCUS       DAYYNQ010000249          367 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000249 DAYYNQ010000000
VERSION     DAYYNQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.260"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>367
                     /locus_tag="ACHBJ1_003036"
     CDS             <1..>367
                     /locus_tag="ACHBJ1_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM9431877.1"
                     /translation="SELEEVVAEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMK
                     NRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYV
                     CFILDLFNREILGYSCGEH"
BASE COUNT          139 a           62 c           71 g           95 t
ORIGIN      
        1 aatcagaact tgaagaagta gtcgctgaag aatttatccg cagccgaaag gcctacggct
       61 caagaaaaat aaaaaaagcc ttatcaaaac gaggcattca gatcagccga cgaaaaatta
      121 gtagaatcat gaaaaataga ggattaaaat cgagctatac tgttgcttat tttaaagtac
      181 atcattctac ttgcaatgaa gccaaaacga caaacgtatt gaatcgtaaa ttcttaagag
      241 acaacccatt agaagcgatc gtaacagact tgacttatgt acgagtcggg aaaaaatgga
      301 attatgtctg tttcattttg gatctgttca atcgagaaat tctcggctat tcttgtggag
      361 aacataa
//
LOCUS       DAYYNQ010000250          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000250 DAYYNQ010000000
VERSION     DAYYNQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.262"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHBJ1_003037"
     CDS             complement(<1..>321)
                     /locus_tag="ACHBJ1_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM9431878.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYNQ010000251          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000251 DAYYNQ010000000
VERSION     DAYYNQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.263"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBJ1_003038"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACHBJ1_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM9431879.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           97 a           45 c           50 g          117 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatat
//
LOCUS       DAYYNQ010000252          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000252 DAYYNQ010000000
VERSION     DAYYNQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.denovo.264"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHBJ1_003039"
     CDS             <1..>282
                     /locus_tag="ACHBJ1_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM9431880.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYNQ010000253         9118 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.105, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000253 DAYYNQ010000000
VERSION     DAYYNQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.105"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(370..1242)
                     /locus_tag="ACHBJ1_003040"
     CDS             complement(370..1242)
                     /locus_tag="ACHBJ1_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM9431881.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1370..1939
                     /locus_tag="ACHBJ1_003041"
     CDS             1370..1939
                     /locus_tag="ACHBJ1_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM9431882.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            2006..2743
                     /locus_tag="ACHBJ1_003042"
     CDS             2006..2743
                     /locus_tag="ACHBJ1_003042"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM9431883.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2974..4242
                     /locus_tag="ACHBJ1_003043"
     CDS             2974..4242
                     /locus_tag="ACHBJ1_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM9431884.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4729..5193
                     /locus_tag="ACHBJ1_003044"
     CDS             4729..5193
                     /locus_tag="ACHBJ1_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM9431885.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5177..5806
                     /locus_tag="ACHBJ1_003045"
     CDS             5177..5806
                     /locus_tag="ACHBJ1_003045"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM9431886.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            6160..7638
                     /gene="msr(C)"
                     /locus_tag="ACHBJ1_003046"
     CDS             6160..7638
                     /gene="msr(C)"
                     /locus_tag="ACHBJ1_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM9431887.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7685..8131
                     /locus_tag="ACHBJ1_003047"
     CDS             7685..8131
                     /locus_tag="ACHBJ1_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM9431888.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8330..8845)
                     /locus_tag="ACHBJ1_003048"
     CDS             complement(8330..8845)
                     /locus_tag="ACHBJ1_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM9431889.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8962..>9118
                     /locus_tag="ACHBJ1_003049"
     CDS             8962..>9118
                     /locus_tag="ACHBJ1_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM9431890.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQL"
BASE COUNT         2997 a         1604 c         1846 g         2671 t
ORIGIN      
        1 tttgattaaa gatctttttg attttcatgc gcgcaccctt tttgaagatt tggttttcac
       61 gtttgctaga ccagttgatt gcggccaaac aaaaatagac acgaaaaacc tcctagggaa
      121 accatgttcc tcagagattt ttcatgccta gtatgatcta gtcacatgaa acgaattatt
      181 gttaacgtaa tcaattttat atagacagtt atgttttgtc aattcttttt ttataattat
      241 agtaatttac tggaattatc agaaataaaa ggaaggaaca aattaagaaa cttatgttat
      301 ttaatcattg gatcttttgc ttaacttatt cgttgttaaa aagctgaaag cgaaggaaaa
      361 aaactctact taactgtatt tctccgttag gacatctaaa aatttacgag cagcaggaga
      421 aagaggctgg tcttttttcc aaatgagatt cagtgaagct tgtaatggcg gatcgagtgg
      481 gatgaatact ttttccgtcc cataagtatt gatgatccca tctagagcta atgcaccaaa
      541 agtcccgtta tcgaccatca aagaagcatt gtacaataag ttatatgttc cgacgatcga
      601 taactgatct atgctgaaac ctagccattc agctagttga ctgtccactg acgtttgccg
      661 ggaaatgatc aaaggaacag ttttcagttc ctgcggatgg atgactgttt gctttgctaa
      721 aggatgattc cgatgtatca aaagtcccca acgatcttgg gctggcagtc gaaatgactg
      781 gtatttttgt ttgtttgtcg gttcgattac gagtccaaaa tcaagtaatc cgctatccag
      841 tttgtctatg atatcatctg cattcccgct atataaatga aactggatat caggatgcgc
      901 cttgatcaac gaatgaaggc tatcagaaat gaaagagatt gctttagttt cgcctccgcc
      961 aatgtagatt tctccagaga tgattgcgtc atttgttaaa ttgttcgccg ttttttctac
     1021 caacgacaag atttcttttc cttttgaata aagatatcga ccagaatccg tcaactgtat
     1081 ctctcgggag cctcgagtga aaagtgtcac accaagttct tcttctaaat ctttcagttg
     1141 ttttgagaga gtaggttgcg tgatatgcag agattttgct gctcggctga tatttttttc
     1201 tctagctact gctaaaaaat acgtcagtat acgaatatcc attttttccc tcctgttttt
     1261 aaaaaagcat accactaagt atagttaaaa ggaatagtta agtatgtttt ataagtatta
     1321 gacatcataa gccgtccgtt ataaagtaag gataaaagga gggagcaaaa tgacaagcct
     1381 catcgaaaaa caaatcgtca ataaagagat tcgagtaacg gatccgctct ttgaagtgat
     1441 ccatcagatc caagcggaaa acgaagagcc tttagcagcg ctgaatactg ggtaccatga
     1501 accagcagat atccgcaaac ggctagaaaa tatcattagt gacaaagtag atgatacggt
     1561 gactgttctg ctgccttttt atacagattt cggcaaacat attagtatag ggaaaaatgt
     1621 ctttatcaat cgtcaagtga tgttcgttga cttaggtggc atctgtttag aagattcagt
     1681 attgattggt cctcgtgtca acctcatcac ggtgaaccat ctaaccgatc caaaagaacg
     1741 aagaggcttg tcagtaaagc caatccacat caagaaaaat gcttggatcg gagcaggagc
     1801 aactattctg ccaggcgtaa cgattggcga gaatgccatt gttgcagcga atgcaactgt
     1861 tacgaaagat gtcccagaca ataccatcgt tgcaggaatc cctgccaaaa tcgtcaaacc
     1921 tgtcgaacgg attatttgaa cagtaagatg aaataagaga tagtgagtat aagcaagaga
     1981 tttttcaaaa caggaggaga atagaatgaa tgatttatca gaaaaagtga ttgtcatcat
     2041 gggcgcttca agcggaatcg gtgaagcaac aacgaagcta ttagcagaaa aaggggctaa
     2101 actagttata gctgcacgcc gagaagatcg cctaaaagca ataaaagaat ctttaccaga
     2161 agctgagcta tatattcaaa cagcagatgt aagagatttt gcacaagtcc aagcagttat
     2221 cgatttagcg atggaaaagt tcggtcgaat cgatgtattg tacaataatg caggaatcat
     2281 gccaaccgct ccattagttg aaggtcaccg tgatgaatgg caaaatatgc ttgatatcaa
     2341 catcatgggt gtgttaaatg gaatttccgc cgtacttcca atcatggaaa aacaaaaatc
     2401 tgggcatatc atttcaactg attctgtggc gggacatgtt gtttatcctg attccgccgt
     2461 ttattgcgga acaaaatttg ctgtccgtgc aatcatggaa ggattacgtc aagagcaacg
     2521 gcaaaataac atcaaatcta cgatcatctc tccaggagct gttcaaactg aattatatca
     2581 aaccatcagc gacaaaaagg ctgctcttga acttcatgaa gcgcaaaaag aatggggatt
     2641 aacatctgaa gatatagctt ctgctgtagc atttgcgata gaaacgccag accggatgtc
     2701 tgtcagcaac atgattatcc gtccgacgac acaggaagta taatccataa cggataatat
     2761 ccagagagaa ttacttgtta aatagttatt caaacgacca tcaatttaag gcgtgaatgg
     2821 agaagaatat tcttcattta cgtcttttta tgcagaaaaa gcaaatttat taaaaagaaa
     2881 caaatgttat ttaaatatgg acattgactt gcttttactt ttttagttac actgttattt
     2941 aggaaaagtt tcataattca aaggagaaaa aagatgaaag caaagaattt attatttagc
     3001 gttattcttt taggaaatct agcagttgcc aacacaacag tgctagctga agaatcgaca
     3061 gaaactgtcg caactcagac atcgagttct tcagaaacag gaattgaaaa cgatacagaa
     3121 agtactttgc agtcagctac tgtctcagaa aatacaattt caaccgaaga accaacaact
     3181 gaaacaacag aacaaacaca aaccgaagac agtacatcag cagaagctca gaacaatcca
     3241 gagaatgcag aagaaatcac gaaccaattg acagatgggc aaacattgac acatacccat
     3301 caagcagatt tgtctgaatt cgaaaactat actggctatg ctttttatgc tcgctcatct
     3361 gctaattctc aacaagcgtt cattgatagt attgcctcaa ctgcacaaag tttggcaagt
     3421 gccaatgact tatatgcatc agtcatgatt gctcaagcca tcgttgaaag tggctggggg
     3481 aacagtactt tggcttctgc ccctaattac aatctatttg ggattaaagg aagctataat
     3541 ggccaatctg ttacgatgcc tacttcggaa tatgtgaatg ggcaatggat tacggtgaat
     3601 gccgctttta gaaaatatcc ttcgtacaaa gaatcattgc aagataacgt aacggtattg
     3661 aagacgactt cttttcagcc aggcgtttac tactattccg gtgcatggaa aagcaatacg
     3721 aactcttaca aagatgctac ggcttggcta acaggtcgtt atgccactgc gcctaattat
     3781 ggttctacat tgaacaatgt aatcgaaacc tataatctga cacagtatga tacagcacca
     3841 acttcaactt ctccgatgta tcgtctgtat aaccgtcata caggagaaca tttatataca
     3901 ttgaatgccg gagaaaaaaa ttatctgcca actgtaggct gggaatatga agggattgct
     3961 tggcaagcac caaacagcgg acaacctgtc taccgtttat acaatcctta cagtggagac
     4021 catcattaca caatggcaca atctgagatc aactctttaa caaaaatcgg ttggcgttat
     4081 gaaggtctaa gtttttattc tggtggttcc aagccaattt atcgactatt taatccaaat
     4141 gaaaaaactg gtacccatca ctataccttg agtgctaagg aaagagattg gctaacaccg
     4201 atgggctgga gatatgaagg aataggattt tatggttatt aaaaagaggt agtgaaaaag
     4261 ctgccctgca tcaagtagta agaacagcca aacaaaaaca gattttcatg ttttttgttt
     4321 ggttgaactt attaccgcag atgcaagttt ttgaacaccg tttactcaaa aaaggttgtg
     4381 acaacatctt gtcataactt ttttattact gtttaagcat actgatccaa tttctaatat
     4441 tcgacgggat tcttgcaaaa ggcgatttcg gaaatttttg gcggtgtaaa attatctact
     4501 gcagaagagc tatcttctat agtttgataa gaatcaataa tcttggtttt agcgttgtgc
     4561 tttctttatc attttttccg ttcattctac aaaaaatttc ttttctctat acttttctat
     4621 tgagagcgtt ttttttatta cacagctgat tctttgactt ttttcttttt ttattctatt
     4681 attgacaagt caatcgttga cgagtcaatt attagaaagg aggaaattat gtctttagaa
     4741 gagattagtc accttttata tcaaatcaaa ttagccgatc aaaaaatcgt ccagatcttt
     4801 gaagaaaata tcggtataag tcttactaga tacgagatca tgatgagact gaaagaagaa
     4861 aatccattgc tacaaagcag tttgcaagaa aagttgcaga ttgatcaagg agcagttact
     4921 cgtcatctga aggtattgga agcaaatggc tatgtttcgc gtcagagaaa tcctcagaac
     4981 aaccgggaag tgtatgtagc gctaacagaa aaagcccaga agaaaatcaa gcattgtgaa
     5041 atggaccata aagatacagg ttccgtgcta tccccggctt tcagcgatgt agaaatgaaa
     5101 caatttctaa cacttttaga taaattgaat acccaacttt gcaatataac aacagaaacg
     5161 aaagaggtta ataataatgg aacaaaaaaa tgatttttca acaattatga ctggtagaag
     5221 atccgttcgc gtatatgatg aaacatacaa aatcccacac gaagaaatgc tggaaatgat
     5281 caaagaagcg acgactgcac cttcttctgt caatatgaaa ccatggcgct ttgtggtcgc
     5341 tgaaagcgag gaagcaaaag aaatcttgcg tcctttgatc cgtttcaata cgagacaaaa
     5401 tgatacttct tctgcaatgg tcatgatttt tggcgatatg caatgttacg aatatggcga
     5461 agaaatctac aaccaagctt acgaatcagg gaagatgcca aaagaagtca gagatcaaca
     5521 attagctgcg atcattcctt attacaagag tttttcaaga gaagaaatga acgatgtagt
     5581 caaagtcgat tctagcttag cggctatgca attcatgtta gttgcgcgtg atcatggcta
     5641 tgaaacaaat ccaattggtg gattcgaagc agatcagttg gcagaggcat ttggtttgga
     5701 taaagaccgt tatgttccag ttattatttt atcaatagga aaagcggtag aagaaggtta
     5761 cgagtctgtt cgtttagcac cagaaaagat cactatgttc gaataaaagg ttttcagtta
     5821 ctattcagta gctattgctt tttttgaatc gtgtgtggta acctaataaa aaaggaggtc
     5881 ggaaaaatga ctgcatcgat gaaattacgt ttcgaacttt tgaataacaa ctaaataaat
     5941 tcaaaagaca tgtataaaca acaatttcat ggatggagta tgtccatttt ttcgaacgac
     6001 agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat ttcgttcaga
     6061 gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt ttttagtatc
     6121 gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt agcagtaaat
     6181 ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga ttctcttcgt
     6241 gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa atcgactttg
     6301 ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac agagatcacc
     6361 ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa tttggaatta
     6421 gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag cggaggggaa
     6481 gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat gcttctagac
     6541 gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga gcttcgttat
     6601 tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga gctagcatcg
     6661 aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta tagtgcctat
     6721 ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc gatcatgaaa
     6781 gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga aaagttggaa
     6841 aaagtgtcca gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc ttcctctaaa
     6901 caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga gagaagactc
     6961 caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt tccagtacca
     7021 aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca attggaaaga
     7081 agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa aaaaatcgcg
     7141 attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg caaacaagga
     7201 gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa gggttatcaa
     7261 atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt ttcagaatcg
     7321 cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac gaaaccgtta
     7381 tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt tactaagcca
     7441 agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac gatcgaagct
     7501 ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca tgattcttac
     7561 tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat aaaaaaagta
     7621 cttacgagaa atttttaaat gtgctacgat gattttcgtg acaaatttaa aggagtggta
     7681 cagattgcct acaaataaaa aagaaggaat aatttttaca acaatgatgt gttttttgat
     7741 ggtcttgggg atgagcgcgt ataatttatg gctgcatcaa gatcttcatt tatcaagttt
     7801 gttgattggt ctagtgcctg gattcgtggt ggctttcatt ttggatgtct tcatcatcgg
     7861 tgtgctggcg aaaaaaattg cctttaaact accagtaaat ccagaaagca aggtccaaat
     7921 gatcttaact atttcatgct tgatggtttt aggaatggtg acatgtatgt ctttattcgg
     7981 tgtgatcatg gaacaaggaa tacctgatca attattctct gcttatatga atgcttggaa
     8041 aatgaatgtg attgccgctt taccgttgca gttgatcatc gttggcccgc tttcaagaag
     8101 tgtgctaaaa gcaattcaaa gtaatgaatg atggaacact gttatttgaa aaaaggcctt
     8161 ggcatgcttt ttgtcatagt ctcttgaatg cagttctaga aaaatagtga tccgaacgat
     8221 aggttttggc ctgtcgttcg gatcgctttt tcttttccaa ctgtattgct cttttattga
     8281 agataatttg ctcttcaata gatatttcta gaaaggttct gtttatccct tactcaaaaa
     8341 actgctgcat ttgttctctt acttgctgac accattggag ttcctgctga caaagcataa
     8401 ttctgcgctg gatcacaaca taacgtccaa attcttgttt cttgtctagc gagaggtcat
     8461 tcaatttttt ttgatttttt tgcatgattt tgttcagctg ttgttctcta gtgaatagca
     8521 gtccagtagc tgttgtttta tcgagcgtcg aaaaagcata gatttttgct agaaattcat
     8581 ctttttgcgt aggagcggga gtttcctctt tgatccattc ctctacaaga gcacgtcctt
     8641 catcagtcaa gtggtagatc tttttttgag agtgctcgtc tttgtccaaa acggtcacat
     8701 aaccctgctg gtctaacttg gcaagcgttg tatagatctg actgtgatgg gcttcccaaa
     8761 aaattgtgat cagctgcttc aattcatacc cagaacgtgg tttggataaa agcacgcata
     8821 ataatatata ggctaaagta ttcataaaaa tcctacctct cgtcttattt tctttattat
     8881 atcacagtaa agtttccacg ttttacatgt gcatattgac atgtaaaata aatcatgtat
     8941 gatgctaata gagagaaagg gatggaaata ttggagaatc aaatagatat acaagaacaa
     9001 acaaatgaag aaacaaaggg gaaaagttca gcgctagttg tagcagctac ctgtatgatc
     9061 gcttttatgg gagtgggtct agtagaccca atcttaaaaa cgattgctgc acagttaa
//
LOCUS       DAYYNQ010000254         7534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.114, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000254 DAYYNQ010000000
VERSION     DAYYNQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.114"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(157..1497)
                     /locus_tag="ACHBJ1_003050"
     CDS             complement(157..1497)
                     /locus_tag="ACHBJ1_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM9431891.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1766..2662
                     /locus_tag="ACHBJ1_003051"
     CDS             1766..2662
                     /locus_tag="ACHBJ1_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM9431892.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2812..3753)
                     /locus_tag="ACHBJ1_003052"
     CDS             complement(2812..3753)
                     /locus_tag="ACHBJ1_003052"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM9431893.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3879..5000
                     /locus_tag="ACHBJ1_003053"
     CDS             3879..5000
                     /locus_tag="ACHBJ1_003053"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM9431894.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5258..6760
                     /gene="eat(A)"
                     /locus_tag="ACHBJ1_003054"
     CDS             5258..6760
                     /gene="eat(A)"
                     /locus_tag="ACHBJ1_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM9431895.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6827..7354
                     /locus_tag="ACHBJ1_003055"
     CDS             6827..7354
                     /locus_tag="ACHBJ1_003055"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM9431896.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2536 a         1373 c         1508 g         2117 t
ORIGIN      
        1 caattatttt gcacagaggg tttttgaaca ccgtttacca agaggtcatg agactgttta
       61 ttcggaaaaa ggagttgcaa caagactttt gtcacaactc ctttcttcaa tagctccatc
      121 tattccacat aaataaaata aaattttcta tcgatttcat tcattcgtca atacgaacgc
      181 atcattcttt ttttgataac tgaaataaat gaacaggtaa agcaggccag tcaatacagt
      241 taccccaatg gagttcgtga acaggatacc aaggaaaatc aatcctaaga cagctaatgc
      301 aaaaaagctg attcctttat cccataatgt tccttttctt agtgccagta caaaggatgc
      361 cgcactgata atgatccaga tcaataatac tgtatacgaa agggaacctg ccagatagcc
      421 aaataatttg tcaccgacaa aataagaaag agctacacca agataaagaa cactggtaca
      481 aaataaaaca gcacgttgcg gtacttgatg cttgtttaaa acagctagtt ttctagagga
      541 accttttcga tccttcaaac ggaagaacaa aattcttgaa gatgcataca cacccgaatt
      601 gatagatgag aacaaagcaa ggacaatcac aaaattgacg atatccgctg caaatgggat
      661 attcattttt tcaaatacca tcacaaaagg actgacattc gtgcctgcta aagtattcca
      721 aggaaaaaga atcaaaagca aaaacaaagg aatgatgtaa aaggaaatga ttcttcccat
      781 gacacctttg attgctttag gaatcgcacg tttaggatca tctgcttcgc ttaccgtgat
      841 agcaatcagt tcagaaccgc cgtaagaata gataactaca agcaaagaac tgatgattcc
      901 cttcatacca tgaggagtaa acccaccatg gtcagtcaat tgctgaaggg tagggaaaac
      961 gcctgtgccc aacatttgct gaccgacaag ataagtagca aaaatgatca gtaagataat
     1021 gacactgatt ttagcaaaag ccagccaata ttctgtttct gcgaataacc gtaccgaata
     1081 aagattgatg atcgtagtta aaacagccaa cataaaaaca aatacccagg ctggaatgtg
     1141 cgggaaccac aactgcaaaa aggaagcagc tgcaacagct tcagcgatga tattgataat
     1201 ccacatagac cagtagaccc aatcagtaaa atcagcagaa tgagcaccta gataagggcg
     1261 tatcaaacca gataatccgt gatgcgtgtc gccaccgctt aaaacgattt ttcccagacc
     1321 tttcatcacg atatataaaa tcagcccacc aatcaaaaaa gcaatcaata cagaagggcc
     1381 ggcgatccca atcgcttcgc cacttccttt aaatagacct gcaccgattg ctccacctaa
     1441 agctaacatg gtgatatgtc tggagctaag ttgttttttc aattttttat cttccataaa
     1501 tgacctcttt cttttaaatt cacaatcaaa atttatatta agaataaata gcaatattta
     1561 acaatgacta atgataacaa attttcagac aattacaact attcagaaaa cttttaggcg
     1621 aattttaata gaagaatgat ttatttcatc atattttcat acaaatgtta aattttcata
     1681 caaatatagg aaacgtttta ctggcttttt actatttaaa caggctatca taggaaaaag
     1741 tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac gtactgctag ttgcaaaaaa
     1801 taaatcactt tattttttgc cttccatccg ctctttagat gtttggtccg gaaaagattt
     1861 actacagtcg gaattcatta aatcagctag acaacagttg gcaaattatc agctgatcgt
     1921 ttttttagat tatggatttg atatggagat ggctcgacgc gtgcgcccat ttaccaatgc
     1981 aaaaattgtg ttattttttt ggaatcactt caaagcagaa cacgaggcga tgctgaagca
     2041 ggcacaagat gaaccggcaa tcgatgagat ttatcatttt gattttttgg aagcaaaaga
     2101 acttgggctg aaacataata gttcatttta ttcgaaacat atgaattttt cttctagcga
     2161 accaaagacg gatctatttt ttggtgcaac gaataatggg agaaaagaac gagcagaaag
     2221 ctataagcag gaatttataa aaagagatgt tacgaccaac tatttcattc ttccacatcg
     2281 agggaatgaa caacctggat atcttactta tgaagagtac ctgaaaaaaa caggggaatc
     2341 gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga gtgactttgc ggacatttga
     2401 atccatgtat tttcaaaaga aactagtgac ggataatcaa gcgattgctc attatcattt
     2461 ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat ttagatgaat taccggaatt
     2521 tctgcataca ccatttaagc caatcctatc agagctattg gaattttatg atgcagaaaa
     2581 ttgggcaaaa cgatttatca ctaacgattc cactatttat gagcaatacg aatatgacct
     2641 ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag agattatgta ctgaaacaaa
     2701 caagcacaat tcttatggca tcatgagagt tgtgttttct ttttgatctt ttattgggct
     2761 taaaaaaaga gaaccagctg ctgattcagc tgattctcat cagagagcct tttattctgc
     2821 gccaaggatt ccttcttttg catggactaa ttgggtcaaa aaggcacaat atggtgtagc
     2881 aatatcatat ttttgtcctt ttcgccaaat ggcaccattg atgtagtcga tttcagtcaa
     2941 acgatggttt ttgatcaggt cttggtacat agatgggtaa tgcaacccga tgttgtcagg
     3001 atcataagta gaagcaatat gttggtacac ttcttcttga tctagtatga ttccttcttt
     3061 tgctgcgata gaagcaaatt cagatacgat tgttcgaacc atgctctctg ccgctttttg
     3121 cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat aaaccattca atgttccatt
     3181 tacacaagct tttcgccaaa tggaataacg aacgttatgg ctatagatcg gatttaaatt
     3241 tgcttcttga aagacttcta caactttttt tgtaaatgct tcgccttctg gttccaagtt
     3301 ctctagttcg atttctccat cacctaacaa tgtgactttt ccgggaccag ctagaccagc
     3361 tgtccacatc gtgatcccaa ataagatatt tttctttggc acatattttt ccagtacatc
     3421 ttcatgacct aatccattta gtaaacacaa aacataagta ttgtcagtaa taatggattg
     3481 gatcgaacaa aacatatcat ctaattgatt ggcttttgtt aatgctacga tcaagtccac
     3541 gtgttcgttg ctttcgatga tttcttctgg tgaatagatt ggcagttttg cgaccacttc
     3601 tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg gcttcaatgt gagcaggcca
     3661 acgatcgatc aagaggacct catttccgct ttgatgtagc atcaatccga ttcggctgcc
     3721 cattgctccg gccccagcta ttgcgatttt catgtttttt ctctccttta ttcaatgagt
     3781 tcccttttag tatatagaaa aatggaagaa aatagcattg ttttgtttga aaaaaatgaa
     3841 aaaaagtgtc ttacttaaga agaaagtgag ggatacagat ggatattttg gcggcaattg
     3901 attcatttaa aggaagtgct tcttcatttg aactaaatca ggcagttcta aatggctggg
     3961 aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga tggcggagaa aatactgcag
     4021 aagcaatcta ttacgcgctc aggggaacat ggcgtaccat tgatgggatg gatctcttat
     4081 tccgtcagcg aaaaatcagc tatttattga caacttggca aggaaaaaac accgcagtca
     4141 tcgaatctgc acaagcattg gggttagatc tacttgataa accaacggat caaactatcc
     4201 gacttgcttc cagttatgcc ttaggagaac ttattaaaga tgcactgctt caagaagtag
     4261 aacaaattat tgtcacttta ggaggaagcg ggtgtagcga tggtggtcta ggattactcc
     4321 aatccttggg tgcttcttta gaagggtaca cagaaggaaa tcctttatta tctactaaaa
     4381 aacttcattt tgatcgaaga aacgttcttg tttctaaagt aaaattgatt atcgctgcag
     4441 atgttaccaa tccatataca ggtcaacaag gagctgcttg gcaatttgga agacaaaaag
     4501 gaggaataga tacgacgcta gctttcttag ataaacaagc aggaaagatt gctcagcaaa
     4561 taaaagattt ctgggaggtg gatttagatg atattccagg aagtggagca gcaggcggta
     4621 ttggtggagc cctttttttg ttgggagcga agatggtttc tggtttcgat ttagtcagcc
     4681 aaatcactgg attggaaaaa aagatcgaac aagcagatgt catcattaca ggagaagggc
     4741 ggatcgatca gcaaacgatc cacggaaaag ttccatacgg tgtagcagtg cttgcaaaaa
     4801 aatatcgaaa accaataatc gctatttgcg gaagtagaga ccaagagcta gaaggacttt
     4861 ctgattttct cccaattgtc ttaagtattc atttgggtcc aataggttta gaagaagcaa
     4921 tggaacacag gcaaacatta gaaaaaacaa aaatcttagg acagacactt agcagatttt
     4981 tcaccatcct cccgaagtag ttgacaaaat cattttcttt atgctatcgt aatttcaaat
     5041 ataggacaaa gtgagggatc cgtgcattga aaaattaagt cgattgatga aagctgcatt
     5101 cagactgtac cttgtggaga caaggaagca gtgtgcagat tttcgggatt ggcttggttt
     5161 tttcgatgta tgggtctttt tgtgtgtatc caacccttgc atcctcttta gccagttctg
     5221 aaaataggac tggtcttttt ttacgaggtg atggaaaatg tctaaaatcg aaataaaaaa
     5281 tctgacattc ggctacgaca gccaaggcac attattattt gaacaagcaa atctaaattt
     5341 tgacacacaa tggaaactag gacttatcgg acgaaacggt cgaggaaaga caactttact
     5401 gaatattcta caaaacaaac taccttatca agggcaagta atccatcagc aagaatttgc
     5461 ctatttcccg caacagacaa aagataaaga acgtttaacc tattacgtgt taaatgatat
     5521 tacggatttt gagatatggg aaatcgaaag agagctccaa ttgatgcaaa cagatcctga
     5581 aatcttatgg agagaattca gcacactatc ggggggagag aagacaaaag tcctactggc
     5641 acttttattt gtggatgaca ctcatttccc gttaatcgat gaaccaacga atcatttgga
     5701 tatctctggt agaaaacaag tagcggctta tttgaaaaag aaaaaacaag gcttcatcgt
     5761 ggtcagccat gaccggggat ttatcgatga agtagtggac catgttttag caatcgaaaa
     5821 aagtcaactg gaactttatc aagggaattt ctctatctat gaagaacaga aaaaacttcg
     5881 tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa gaagtcagta ggctaaagaa
     5941 aacagcagct gaaaaagccg aatggtctcg ttcccgagaa ggagataaaa caaagaaaca
     6001 agtcggattc atcgatactg aatctagacg agtgaataaa ggagcagtgg gtgctgatgc
     6061 tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg atggagaccc agatcagcga
     6121 gaaagaaaaa ctattaaaag atatcgaata tatcgattct ttgacgatga atagccaagc
     6181 gtctcaccat aagcgacttt taagcgtaga agatcttcaa ttagggtatg aaaatctgtt
     6241 attcgagcca attcatttta caatcgagcc tcatcagcgg gtggcgattt caggtcctaa
     6301 cggtgcagga aagtcatcca ttatccatta tcttctgggg gcattcaacg gcaaggttat
     6361 aggagaaaaa taccagccaa aacatctgag cattagttat gcaagccaaa attatgaaga
     6421 caatcgagga acgttggcgg aatttgcaga gaaaaaccaa gtagactacc aagcattttt
     6481 gaacaacctc cgaaagcttg ggatggaaag agatgttttt cataacaaga tcgagcagat
     6541 gagtatgggc caacggaaaa aagtggaatt ggctaaatct ttatcacagc cagctgaact
     6601 atatacatgg gatgaaccat tgaattattt ggatgtcttc aatcaagaac aattagaaca
     6661 actgatcttg aacgtgaaac ctgccatgtt actagtggaa catgatcaaa ccttcctgga
     6721 taaagtatct actgagatta tttctcttga gagaatctaa cccttgtaat ctcaagaaca
     6781 acaaggtatg gttaagggga actttgccaa cgaaggagag aatcacatgc tttatgtaac
     6841 gagacacgga gaaacaactt ggaacgcaca aggattagtt tgcggacggg cagatgtgcc
     6901 gctgactgaa aaaggacaga tgcaggcaca gaaactagca gaaaaggtag tagatttgcc
     6961 tgttccaatc acgaaaatca tccattcccc tcttcaacga gcaagagata cagcacaagc
     7021 agttgctgat cgtctcagct tgccgctgac cgttgatgaa cgattggtag aaatggattt
     7081 tggggattat gatggactgc caagcaaaga tgagaacttt caaaaagcac gtttagcgtt
     7141 cgctgtacga ttcccaaatg gggagtcagt cttagatgtg tatgcacgga tcgttccttt
     7201 attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg ttagtttgcc ataatgcctt
     7261 gataagagtg atcaatgctt attttcatcc gatgcctaat gaggggttct ttacattcat
     7321 ggtagataat acagaattgg tttcttatga gtaattatgt ttttgtgtta tttaaataaa
     7381 atatttcaag gcactgcttt tgcttagcag tgcctttttt aggttatgac aattcttttg
     7441 ataatataaa ctcaaccttt agaaatgaaa tttaattatt ttaataaaag tattgaaatt
     7501 ttgtttcttt agatatatac tctagtcata gaaa
//
LOCUS       DAYYNQ010000255         6011 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.130, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000255 DAYYNQ010000000
VERSION     DAYYNQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.130"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1224)
                     /locus_tag="ACHBJ1_003056"
     CDS             complement(<1..1224)
                     /locus_tag="ACHBJ1_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM9431897.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYG"
     gene            1503..2309
                     /locus_tag="ACHBJ1_003057"
     CDS             1503..2309
                     /locus_tag="ACHBJ1_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM9431898.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2327..3034
                     /locus_tag="ACHBJ1_003058"
     CDS             2327..3034
                     /locus_tag="ACHBJ1_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM9431899.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3031..4002
                     /locus_tag="ACHBJ1_003059"
     CDS             3031..4002
                     /locus_tag="ACHBJ1_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM9431900.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3995..5023
                     /gene="vanB"
                     /locus_tag="ACHBJ1_003060"
     CDS             3995..5023
                     /gene="vanB"
                     /locus_tag="ACHBJ1_003060"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM9431901.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5029..5637
                     /locus_tag="ACHBJ1_003061"
     CDS             5029..5637
                     /locus_tag="ACHBJ1_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM9431902.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5703..5876)
                     /locus_tag="ACHBJ1_003062"
     CDS             complement(5703..5876)
                     /locus_tag="ACHBJ1_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431903.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1701 a         1233 c         1513 g         1564 t
ORIGIN      
        1 gccataagcg actcgtttca atgctttgat gtgggtatgc agattttcta ttttggcatt
       61 tgaataaggc aattcgaggg ctaaacgaat gccggattca tagtttataa aagctttttt
      121 tatagaatgc agttcttttg gaacggaatt gggcatcgtt cttacaagtt taaagaaagt
      181 gtccgcctct ttgtcgtgaa acgcttgaag aatttcttga tagtaaaaat aggcgattcg
      241 taattcgtca gagatcgaaa gcagacgatc caccacttca ctttcggtga gatacgccca
      301 accaaaattg cgccatttgg tccatttgtc ataaacaagc ttgtcctgtc gtttgatcag
      361 aagccgagac aacgccttta attgtcgata atatttggct tgtgttggat cgtttcgatc
      421 aaagcttttc atgattcgta cgcgaatatc ctcaaattta cgaatgagat gtttgacaat
      481 gtgaaaacga tccacaacga gttttgcctt tggaaaacat tcctttatca gagaggcata
      541 agaaacattc atgtccgtca cgatgacctt taccttcaat cgtgcgcgtc gttcaaattg
      601 cataaaataa gaaacgagag cctttttctt ccgtgaagga agaatatcca gtaattttcc
      661 agtatcgcca tccatacaag aaaaggagta gggaccgact tgatttttca gtgaacgaaa
      721 ttcatccatt aataaacaac gcggtaacca atttcttttt acttttgtca tggggatcag
      781 agaagcgagt gtccgagaaa cggtcgacca agaaacgccg caatgcttag caattgtcgt
      841 cattgcttgt ttttcagtta gctcttctaa aattttatag cgcacttgtt tagcgataaa
      901 gcagtttcta tcgacaatgt aggtattcgc cgtaaaatag gtgtggcagc cacgacagtg
      961 gtagcgttgt ttcgctaaag caagataagt cagttggttc gcacatttat taagttgaat
     1021 gagcgatttt ttctttccgt tcttaacgat catgccttca tgattcatct gtttacacaa
     1081 aggacagcga cgaatttctt tacttaacgt cgcagaaaca acaagactct tgatattctt
     1141 aatgctcttc gattccacat ttgtgatgaa aatgttttta tctttgattc caagtagtag
     1201 tttgatatga tgaagatcag gcatacagaa cgctcctttg attgtgttgt ggtgattcaa
     1261 ttctaacgga gcttctgtat gttttgtttt ttcatgctca aaagggaccg atagaatgat
     1321 tttctatcag tcctagaaat tatagagccg attttgaccg acaggtataa ccctgccggt
     1381 ctttttttgc tgctacagga aaactacaga ctggctacag ggaaagtaca gatgcgcttg
     1441 ccataataac aatcgtacca gccacaaatc gtagttttat tgcaaaggag gcattcgatc
     1501 aaatggaaaa aagcaactat cattccaatg cggatcatca caaacggcat atgaaacaat
     1561 ccgtggaaaa gcgggctttt ctatgtgcgt tcattatttc gttcacagtc tgcacgctgt
     1621 ttttggggtg gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa
     1681 ctcatacagg cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg
     1741 ccaaagaaca gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca
     1801 tccccgcaca gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc
     1861 ggatttctcc ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga
     1921 ttgtcgcatc cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg
     1981 ccgaatataa ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg
     2041 tggccgtgcc gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg
     2101 gaattcattc aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg
     2161 gttttattcg tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt
     2221 ggcattaccg atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc
     2281 ttgaggaata tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag
     2341 attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt
     2401 ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct
     2461 ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat
     2521 gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt
     2581 attgataaga ccgggggaaa ccttttcttt ctggttgttc ctgcatacgc cattgacaat
     2641 tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa
     2701 aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga
     2761 ctgcacctac caaatatggg tgaccttaga tgatgagaaa atcatcggtc aggtgttcgc
     2821 cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga
     2881 aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg
     2941 ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc
     3001 ggaaagtgtg gatattcagg aggagaacca atgagaaaaa gtgtgggcat tactgttttt
     3061 ggatgcgaac aggatgaggc aaatgctttc cgcgctttat cgccggattt tcatattatc
     3121 cctacgctga ttagcgatgc gatatcggca gacaacgcaa aattggccgc tggcaatcaa
     3181 tgcgttagcg taagccataa gtccgaggtt tccgaggcga caattcttgc gctgagaaag
     3241 gtcggggtaa aatacatttc tacccgcagc atcggctgcg atcacattga tacgactgcc
     3301 gccgagagaa tgggaatctc ggttggcacg gttgcgtatt cgccggacag cgttgcggat
     3361 tatgctttga tgctgatgct gatggccata cggggtgcaa aacccaccat gcacgccgtg
     3421 gcgcaacaag atttcagatt ggatcgtatc cgggggaaag aactggggga tatgactgtg
     3481 ggagttattg gaaccggcca tatcgggcaa gcggtcgtca aaaggctgcg gggatttgga
     3541 tgccatgtgc tggcctatga taacagccga aaaatggatg cagattatgt ccagcttgat
     3601 gagcttctaa aaaacagcga tattgttacg ctccatgtgc cgctttgtgc ggatacccgc
     3661 catctgatcg gtcagaagca aattggagag atgaagcaag gcgcattttt aatcaacacc
     3721 gggcgcggag cgcttgtcga taccggggcg ctggtggagg cgttggaaag cggaaagctg
     3781 ggtggtgcgg cactggatgt gttggagggc gaggatcagt ttgtttatac cgattgctcg
     3841 cagaaagtgc ttgaccatcc ctttttgtcg cagcttttaa ggatgccgaa tgtgatcatc
     3901 acaccccata cggcgtacta cactgagcgt gtgctgcagg atactacaga aaaaacaatc
     3961 aggaattgtc tcaattttga aaggagttta cagcatgaat agaataaaag tcgcaatcat
     4021 cttcggcggt tgctcggagg aacatgatgt gtcggtaaaa gccgcaatag aaattgctgc
     4081 gaacattgat acggaaaaat tcgatccgca ctacatcgga attacaaaaa acggtgtatg
     4141 gaagctatgc aagaagccat gtacggaatg ggaagccgac agtctccccg ccatactctc
     4201 cccggatagg aaaacgcatg ggctgcttgt catgaaagaa agcgaatacg aaacacggcg
     4261 tattgatgtg gctttcccgg ttttgcatgg caaatgcggg gaggatggtg cgatacaggg
     4321 gctgtttgta ttgtctggta tcccctatgt gggctgtgat attcaaagct ccgcagcttg
     4381 catggacaaa tcactggcct acattcttac aaaaaatgcg ggcatcgccg ttcccgaatt
     4441 tcaaatgatt gataaaggtg acaagccgga ggcgggtgcg cttacctacc ctgtctttgt
     4501 gaagccggca cggtcaggtt cgtcctttgg cgtaaccaaa gtaaacggta cggaagaact
     4561 taacgctgcg atagaagcgg caggacaata tgatggaaaa atcttaattg agcaagcgat
     4621 ttcgggctgt gaggtcgggt gtgcggtcat ggggaacgag gatgatttga ttgtcggcga
     4681 agtggatcaa atccggctga gccacggtat cttccgcatc catcaggaaa acgagccgga
     4741 aaaaggctca gaaaatgcga tgattacagt tcccgcagac attccggtcg aggaacgaaa
     4801 tcgggtgcag gaaacggcaa agaaagtata tcgggtgctt ggatgcagag ggcttgcccg
     4861 tgttgatctt tttttgcagg aggatggcgg catcgttcta aatgaggtca ataccctgcc
     4921 cggttttaca tcgtacagcc gctacccacg tatgatggcc gccgcaggaa tcacgcttcc
     4981 tgcactgatt gacagcctga ttacattggc gttaaagagg tgataagtat ggaaaatggt
     5041 tttttgtttt tagatgaaat gttgcatggc gtccgttggg atgccaagta cgccacatgg
     5101 gataacttca caggaaaacc ggtggatggg tatgaggtga atcgcatcat cggcacaaag
     5161 gccgtggcgt ttgctctgcg cgaggcacaa atccatgcgg ctgcgcttgg ctatggcttg
     5221 cttttatggg atggatatcg gccaagaact gcggtggact gcttcctgcg ttgggcagcg
     5281 caaccggagg acaatctcac aaaagaaaaa ttttacccca atatagagcg agccgagttg
     5341 attacaaagg gttatgtggc ctcacaatcc agccatagcc gtggaagcgc aattgatctt
     5401 acgctctacc acctggatac aggggaactt gtttcaatgg gaagtaactt cgattttatg
     5461 gacgaacggt cgcatcatac agcaaaaggg ataggggatg cagaggcaca aaatcgaaga
     5521 tgcttgcgta aaatcatgga aagcagcgga tttcagtctt atcgctttga atggtggcac
     5581 tataagttga ttgatgagcc ataccccgat acctatttta attttgccgt ttcataatga
     5641 aagtattggt tttgtatcag attttggatt tgataaaatc tatgctggtc atggacaatc
     5701 ttttactgga aagtggttaa agcatctttt gtaatacatt gcaaattata gtattcaatc
     5761 tgcccagcgg cagaaaagaa aaaaaccgct gttgggcaga cctttcccac gcccattttg
     5821 tattttgctg gcggcggttt tgagaaatca aggccgccgt tcttttctgg cagcaaaagg
     5881 tgtggcggcc cataggagga tgccgctatg ctgttttgct attgccatgc tctaatccac
     5941 ttccaccact caaaaaaagc cttgcccctc catgaactga accccaaaag ttgaactatt
     6001 taatggactg t
//
LOCUS       DAYYNQ010000256         4821 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000256 DAYYNQ010000000
VERSION     DAYYNQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.149"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..171)
                     /locus_tag="ACHBJ1_003063"
     CDS             complement(<1..171)
                     /locus_tag="ACHBJ1_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431904.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCL"
     gene            683..1318
                     /locus_tag="ACHBJ1_003064"
     CDS             683..1318
                     /locus_tag="ACHBJ1_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM9431905.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1454..1546
                     /locus_tag="ACHBJ1_003065"
                     /pseudo
     CDS             1454..1546
                     /locus_tag="ACHBJ1_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            1660..2295
                     /locus_tag="ACHBJ1_003066"
     CDS             1660..2295
                     /locus_tag="ACHBJ1_003066"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM9431906.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            2402..3640
                     /gene="mef(H)"
                     /locus_tag="ACHBJ1_003067"
                     /gene_synonym="mefH"
     CDS             2402..3640
                     /gene="mef(H)"
                     /locus_tag="ACHBJ1_003067"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM9431907.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            3867..4286
                     /locus_tag="ACHBJ1_003068"
     CDS             3867..4286
                     /locus_tag="ACHBJ1_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM9431908.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            4291..4530
                     /locus_tag="ACHBJ1_003069"
     CDS             4291..4530
                     /locus_tag="ACHBJ1_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM9431909.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         1595 a          715 c          913 g         1598 t
ORIGIN      
        1 cagacaacgc aggataatgt tctgtgtttc cttgctggcg ttagcttttc cgttctcaaa
       61 gcggttgata ctatatcttg atacatgggt aagacttgca agttctcctt gtgaaagacc
      121 gctggcaaga cgtttgttct tcatttcaag tgcaaattct aaattgttca ttgtattcct
      181 ccaatcccac aatagccttg aaatacctgt aaaccacctt tcaaaaagtg tgatatgtac
      241 tatcttctta aaacctctca ttactactca caggtttggg ggtgtatggt ttttgacttg
      301 tcttttgtga aatagtgccc ccctgttaga taacgggggc acagacaagg ctcgcaggct
      361 cgccagccga caaagcattt cttccccttg acacaaggtg tcatggcggt aagcctgaga
      421 ggcttccgcc accgtgtcag aaatactttg tcagcaagtt ctgttttgcc ttttctaaca
      481 agttccattt caaccgatat tcatatctac ggatttaatc agataggtat ttctttttga
      541 atgtttttat atttcattct attgtgctta ttcgattaca agaacatagt aattcttata
      601 tgcttatttg tcaatatctt tttttgcctt tacaaatcat attaagcatg atagacttat
      661 aatagcgaag atataacgag atatggaggg ttatgcaatg aaaataagcc atttaggaaa
      721 taatatacag accataagaa aatttagggg aatgaaacaa caggaacttg cggacaaaat
      781 cggtatcaat atgcagagcc tttccaagat tgaaagaggg ttgaactatc ctgcctatga
      841 aacgctggaa aagataatgg aagtgctgga cgttacgccc aatgaattgt tgtcgggaga
      901 atggaagtat gtcaatcaat ctgaaaagga agtctgtcag tttttaagag ccgaagaacg
      961 tctaaatgca gaactgaaac atggacacta tgataacttc tttgacagcg aggaagaatg
     1021 gctggaatat gagttagaaa agttacggga atatattaca gactatatca acgggaaaag
     1081 cattgaagcg tccgaccttt acccaatcaa agaatttatc cagcatttga agtttcaaaa
     1141 attattagac cgctatgatg atttatacag tatggatatg tttggggaaa gcatagaggg
     1201 acataagtat aagacacctt atcaagtcgt gaaaatgata aacccgaact caaaagaaga
     1261 tatggaactg ttacgagaag tattaaggaa taacaatttt gatgatgagg acgagtaact
     1321 tttttcctct tgcgagaaga tacagcaaac aaaaagcccg atcaagagtg caaagcacca
     1381 ccaatggtgg caaagctctt gacagggctt tttcttttct gttgtgggca atcaagagga
     1441 agaaagggat aaaatctgat ttgtggtaaa tcaggcaaag gcaacgcagg tatcacagta
     1501 tgaattgaca ccgcataagg aaagcaactg gaagattgta gaataaatga aaaaggcttg
     1561 cattctgcat ttttataaaa ttgcaagtct ttatattgac aagcataaaa tactaagata
     1621 tactttaatc agaccaacgg tttgtattgg aggtaagtta tggcacgaaa taaatatcca
     1681 gaagaaacag taaaactcat tttagatgtt tctacaaaaa tgttttgtga aaaaggatat
     1741 gataacacat cattgcaaga tattatcaat gaaacaaaac tttctaaagg tgctatttat
     1801 catcatttcg attcaaagga agatatttta aaagcaattt ttcaaagaat tggaaatgaa
     1861 aatgcagtaa tatttgctaa aattagagat aacgatagtt taaacggttt agaaaagtta
     1921 agagaaatat ttaagacagc ggtattcaat tctaaccaaa gcgttttatt gacagtttct
     1981 cctcaactat taaacaatcc tcgttttctt gctatgcaaa tagaacaaat gtatgaaatt
     2041 gtagtacctg aatttgttga acccgtttta gaacaaggta tcaaggataa gtctttggag
     2101 attgaaaatt cacatgaaat tgcagaagtt atcatggtat taagtaatgt atggctcaac
     2161 ccattagttg aaatgacgga taaagagggt atggaaaaac gttgcaatac attcaatgcc
     2221 cttttaaatg gaataggcat agacacatta ttagacgaag aaacaatttc tgcatttata
     2281 cagttttgta aataactgta tttgttatcc ctatacgata gactgctcta taaaagagca
     2341 gtttatttaa accattatat aaaccgacgg tctgtttgat taaataaaaa ggagagattt
     2401 tatgcaagca gaaaaaaaac tttttaatcg tgattttata cttgttgtta taggacagat
     2461 aatttcttta tttggaaacg ctatcctacg atttgcatta ccgtactatt tgttaaaaga
     2521 aactggttca tcagctttgt ttggtattgc tacagcctgc tcttttttac ctatggttat
     2581 tttgtcgctg gtaggtggta ttattgctga caggacaaat aaaagaaaca ttatggtcat
     2641 actagatttt gtaacagcaa tcataatttt agtattttat ttgtcattgg gaaaaattcc
     2701 tatcatacct ttgtttattg ttgctttgat gttactttat ggtatttctg gtgcatatca
     2761 accagcggta caagcaagta ttcctctatt ggtatcatca gataaactta ccgtcggtaa
     2821 tgctataata aatcaagtaa atactttagc aaactttatc ggaccagcta taagcggagt
     2881 agtttttcaa ttttatggta tcaatcccat tttaattgtt agtattatat gttttacttt
     2941 ttcagccatt atggaaattt ttatccatat tccacaccaa aaaagacctc gtgaagtagg
     3001 tgtaattgca gttgctaaaa acgatttaaa agatagttat acctttttga aaaaggagaa
     3061 acccattttt atatccgtaa tctttttggt ttgcattttc aatttggttt taagtgctgt
     3121 tttaattgtt ggtctgccta tcttaatttc tgaagtttta aaaatgccag atacactata
     3181 tggtttttca caatcagcat tatcattagg tggtttatgt ggtggtattt taacagctct
     3241 tgttgcttca aagttaaaac taaaaaacct ttataccttg ttacttattt gttctgtttc
     3301 tgttttactt atgggaattt cgctattttt agaattgcct gcaatagttt cctactgggt
     3361 agttacagtg atgtgctttg tggctatggg tgtatctact atgtttgttg ttcaaattta
     3421 cactcttgtt cagacacaaa caccaccgca attagtagga aagataatgg ctacgctggt
     3481 agcagttgct atgtgcggtc aacctattgg acaaatggtt tacggtattt tattcgatgt
     3541 atttatacaa gaaacatggg ttgttatgtt tgtagcttct attgcttcaa tcatgatttc
     3601 aatatattca aaaagaattt ttcaaaagtt agagatataa tacaaggggt taaatagtat
     3661 gttaggctaa aaacaatata taaaagaaaa tttgaaatct gctaaaagga tagtgttata
     3721 aaagaaagag taatattgca gtatttaagg ctcgcacaaa tatatgtttt gtgtgggctt
     3781 ttttcttacc atttttctgt atttctaaag ggtataaacg gtattccgtt tatatatttc
     3841 agcattagca gaaagggggt gagattatga ggacatcttc tttcgagcat atcgtcagat
     3901 tacagttcaa ttctttaatg ctgattgtca ttaagaacaa agtgaaaagt cgaaacagac
     3961 agcttgcaag acattctaaa cgtgaagtat tgttttgtga aatagccgaa acgaaacaag
     4021 ttgaacgtgg aacgattgat acctgttttt atgattgtgt atcatttaaa gtcttgcact
     4081 ttacaattta tgtatctgat gaaacacttg gtacagtttt acatagactg tcagaaaaac
     4141 agcgtagtgc gattttatta cgttattttc aaggaatgaa cgacagagaa atttcagaac
     4201 tgtaccatgt atcacgttct gctatttgca gtagaagaac tagaggacta aggaagttaa
     4261 aaatgctttt gagcgaaagg aagtaaagaa atgagtaaag attatggcta tccctctttt
     4321 gaattgattt gcaaagcgtc aagcggagat gaaatggcag ttaaagagat tttgaagttt
     4381 tatgacggtt atatttctaa attatgcttg cgtccttttt accactctga aagtggtaaa
     4441 atcattatgc aggttgatga agaactaaaa ggagaaattc atacagacat gatgaaagca
     4501 atattgaaat ttgaaatcgg agtgaagtaa tcaactgtat aaaaaaatcg ggagatacac
     4561 agtttcgtgc tatctccctt agtagtttta cggtcatatc aaggctcatt caatcgtggt
     4621 aaattcgtgg taagatgagt gtttttctat acttcaaaat gcttgaatgt atagtgttta
     4681 tgcggataat tgaaaattag atataaaatt tcaaataaat tactgatata aatttacaat
     4741 aagaactctt gtttctactt ttttttatga gaaaatttag ttttcaattt attgaaccat
     4801 tttcggcttg gatctcgacg t
//
LOCUS       DAYYNQ010000257         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.162, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000257 DAYYNQ010000000
VERSION     DAYYNQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.162"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACHBJ1_003070"
     CDS             <1..308
                     /locus_tag="ACHBJ1_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM9431910.1"
                     /translation="DQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTA
                     SISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            538..1605
                     /gene="rlmN"
                     /locus_tag="ACHBJ1_003071"
     CDS             538..1605
                     /gene="rlmN"
                     /locus_tag="ACHBJ1_003071"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM9431911.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            1595..1801
                     /locus_tag="ACHBJ1_003072"
     CDS             1595..1801
                     /locus_tag="ACHBJ1_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431912.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(1798..2901)
                     /locus_tag="ACHBJ1_003073"
     CDS             complement(1798..2901)
                     /locus_tag="ACHBJ1_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM9431913.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3453..4001
                     /gene="aac(6')"
                     /locus_tag="ACHBJ1_003074"
     CDS             3453..4001
                     /gene="aac(6')"
                     /locus_tag="ACHBJ1_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM9431914.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1349 a          692 c          834 g         1191 t
ORIGIN      
        1 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
       61 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
      121 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
      181 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
      241 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
      301 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
      361 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
      421 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
      481 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
      541 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
      601 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
      661 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
      721 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
      781 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
      841 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
      901 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
      961 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     1021 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     1081 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     1141 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     1201 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     1261 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     1321 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     1381 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     1441 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     1501 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     1561 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     1621 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     1681 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     1741 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     1801 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     1861 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     1921 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     1981 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     2041 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     2101 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     2161 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     2221 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     2281 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     2341 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     2401 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     2461 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     2521 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
     2581 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
     2641 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
     2701 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
     2761 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
     2821 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
     2881 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
     2941 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
     3001 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
     3061 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
     3121 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
     3181 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
     3241 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
     3301 gaataaagtg tttctgtata attttttatt ttataagtaa gataaataat gaaagaatag
     3361 agatactgat tggtagctaa agatttttga gcgagaacat ttcgtttttt caaaaggact
     3421 gttagaataa gcaagaacaa gggggaagac gtatgataat cagtgaattt gaccgtaata
     3481 atccagtatt gaaagatcag ctttctgatt tactgagact gacttggccg gaagaatatg
     3541 gagacagctc ggcagaagaa gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag
     3601 cggtagacca agatgagtta gtaggattta ttggtgcaat ccctcaatac ggtatcacag
     3661 gttgggaatt gcatccatta gttgtagaaa gctcccgacg aaagaaccaa ataggtactc
     3721 gattagtcaa ttacttagaa aaagaagtag cttccagagg aggaatcacg atttatttag
     3781 gtacggatga tttagaccat ggaacaacgt taagtcaaac ggacctgtat gagcatacat
     3841 ttgataaagt ggcttccatc cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat
     3901 taggttataa aatcgtaggt gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt
     3961 ggatggcaaa aacgattatt cctcgaccaa attctcaata ataaaaagag gttgtgacag
     4021 aagtaatcag cttcaagaaa taagaaggaa tttacgaaaa ttactt
//
LOCUS       DAYYNQ010000258         3567 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000258 DAYYNQ010000000
VERSION     DAYYNQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.171"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            217..909
                     /locus_tag="ACHBJ1_003075"
     CDS             217..909
                     /locus_tag="ACHBJ1_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM9431915.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1236..1733
                     /gene="dfrG"
                     /locus_tag="ACHBJ1_003076"
     CDS             1236..1733
                     /gene="dfrG"
                     /locus_tag="ACHBJ1_003076"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM9431916.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1805..>3567)
                     /locus_tag="ACHBJ1_003077"
     CDS             complement(1805..>3567)
                     /locus_tag="ACHBJ1_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM9431917.1"
                     /translation="QYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKDKGHKGM
                     YCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGCSEEEST
                     PFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPMFAKMVV
                     GRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWLNTDKMH
                     YYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISMDELNED
                     TRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRFVDGLNV
                     DAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNK
                     TKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDG
                     ERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQI
                     RRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTP
                     AQRLGITDKQFDWKDIIYFK"
BASE COUNT         1238 a          568 c          619 g         1142 t
ORIGIN      
        1 ttttgttttt gttcaccata aatatttatt taagaaagta ttaacctgca tttttctcta
       61 taaaaaagat tttttaaata gataaaaaag gaggattaaa tgagtatttg atagtattta
      121 tattaaaatt acatatgtaa tttaaaaacg cttgacattg tgtttgagat cacttacttt
      181 tagcatgtaa aagtattgga ttaaggagct atgcaaatga aaaagaaatt tattactggt
      241 gggattgtcg ttgcatttct atgtctaatt ggctacacag gcacgcggta cgcaaatgaa
      301 attattttgt ggggtggaga gacagatatt gatacaatca atgagaatct gacacagctt
      361 gatcatacat tgacagataa agagcaagcg attacagatc ttaccgctcg actagcacaa
      421 aaagaacaga cattgcaaga ttcaaaggca gatgtccaag aataccaaac aaaagtaaat
      481 gaattagaaa accaaaaaaa tcaatcggca gcagaaaaac aaaacttaga aagtcaatta
      541 gtcagcaagg atgcacaact acaaagcaaa caaaatgaaa tcaatgtcaa actagatgag
      601 atcaaccaaa aaaatgatga aatcaatcat ctgaatgcag attggacagc aagactacaa
      661 gaagcacaag caaaactttc agaattacaa aatactgtaa atggtttgaa tcaacaaatc
      721 gcaacactga cacaagaaaa aacaaatgta gaaactcagt taacagatac acaaaagaaa
      781 ttagacgaag caactgctga aaactcaagc tggaaacaat atattcaaaa gttagaaaaa
      841 gcaaaagatg aagtggaaga tacagctgat aaatctcagc aaattgttga agaacatttg
      901 cgtaaataat ctttcttaac aagtaaacct aaattagtaa agaagcaaca gtaatctata
      961 ctaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt
     1021 ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
     1081 gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa
     1141 ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga aataagaaat
     1201 tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga
     1261 tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact
     1321 gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg
     1381 aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt
     1441 ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa
     1501 acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg
     1561 ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag
     1621 aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa
     1681 atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc
     1741 gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt
     1801 tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc
     1861 tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt
     1921 aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat
     1981 agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc
     2041 ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga
     2101 tttcgtttgg ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat
     2161 ccttagtggc taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat
     2221 ctttactaat agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag
     2281 atgccaaagt ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt
     2341 ctgcttttgc ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt
     2401 ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg
     2461 cgtttctaat agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt
     2521 gtttaattaa ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga
     2581 caaatctact cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc
     2641 tatcgttagc ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac
     2701 taaaagtatt gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg
     2761 aaatattcca atcataggca acatcagaac ggaacacata tcttgataag acctcagcag
     2821 atacaactat atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt
     2881 gtcctttctt acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct
     2941 cgttaaattt ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac
     3001 gcctatacag ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac
     3061 aagtacgact gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt
     3121 tcttttgttg gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct
     3181 tacacgcctt acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt
     3241 gcttcggctc attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat
     3301 aatctggctc aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa
     3361 cagaccaatt agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat
     3421 tgtcattgca gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc
     3481 gacttggttt cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa
     3541 acggattgat gcagtaatta tattgaa
//
LOCUS       DAYYNQ010000259         3552 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.172, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000259 DAYYNQ010000000
VERSION     DAYYNQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.172"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..138)
                     /locus_tag="ACHBJ1_003078"
     CDS             complement(<1..138)
                     /locus_tag="ACHBJ1_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM9431918.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IE"
     gene            283..468
                     /locus_tag="ACHBJ1_003079"
     CDS             283..468
                     /locus_tag="ACHBJ1_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM9431919.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            626..793
                     /locus_tag="ACHBJ1_003080"
     CDS             626..793
                     /locus_tag="ACHBJ1_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431920.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACHBJ1_003081"
     CDS             1411..2070
                     /gene="vanR-B"
                     /locus_tag="ACHBJ1_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM9431921.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            2073..3434
                     /gene="vanS-B"
                     /locus_tag="ACHBJ1_003082"
     CDS             2073..3434
                     /gene="vanS-B"
                     /locus_tag="ACHBJ1_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM9431922.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
BASE COUNT         1041 a          697 c          880 g          934 t
ORIGIN      
        1 ttcgatgttg gcaagatagc gccagtcaat atttattttc tctgccattg ctttgcgcga
       61 aacgcgcttt gcttgccggg ccgctttcac atccgcgccg aaagtttcaa agccgggaca
      121 atcttcaata ttcgccatat cgcacctccc acttacattg tatatttcat actctgcccg
      181 tggaatgttg gtatatgcac ctttcttaaa gtttatattt cacattttgt ttcttttatt
      241 cttcgagcaa aagaattata atgtaccctg tggaggtgta ctatggacta catgacgttg
      301 aaagaggcag gcgagaaatg gggtgtgccc ccacgatgga taaactacta ttgtgctgcg
      361 ggacgcatcc ccggcgctgt aaagatggcg acgatctggc tcattcccaa aactgcggaa
      421 aagccgattg acaggcgtac aaagaaagga aaggcggtta ctgaatagta taatgaaacc
      481 ggcgcatttt ggatatggtt ttgaagagag agaaagcatg gtattttgtc acggaaaaac
      541 tataagatga tagatggcag gctgctccag accgataaaa agtattctca tctgaaacag
      601 aaagaaaaga tttacacatg gatgtatgaa gaagcgaaac tttatcatga cagaaatgga
      661 aaatgcccga aacaaagacg aggaagctgc tgctgtatca gccgtatatg gtagaattga
      721 aaaggccggc atctggattc tttatggtga agtctccaga cattggataa ctgctggtct
      781 aatgcgcctt tgagtatggg gtgattgatt ttaataccgg ggaggtacgg ctttttgagg
      841 aaaatattct gggatttaca tttggcgatt attacatttc ctgtaactgc aaggacacgg
      901 atgatgaaga tatgaggaat atagacttgc tgattttcta ctgtcttgaa cggtattagg
      961 actgaatctg ctgcgcggag tgcggcaaga gcgaccggag ggagtacaca caggccctgc
     1021 ctgtgtgaaa agccataccg caaggtgcat tcgcaaaaaa gaggccgcgc attttgctga
     1081 ggcttggggt gtccccaaca agcaaaacga gaacgccgca ggcgggcaag tggtggatca
     1141 gaatatccgt aaaggatatt ctgatccatt gcttgccagt attatcggga tgttttgaca
     1201 accgctgtaa tcgaaacatc ccaaacatta agaaggtgtt aattattatt atcttgattt
     1261 aattcgttta aaccaataat atagatacat acaccatgaa aagaagcgag tatcccaatg
     1321 acggatgatt aaaatccgaa aagaaatcgc tttgaaacta cagggaaact acagactgtt
     1381 atgttatctt cttaaaatgg agggattttt atgtcgatac gaattctact tgtcgaggat
     1441 gatgatcata tctgtaatac agtaagggcg tttctggctg aggcaggata tcaggtggat
     1501 gcctgcacag atggaaatga ggcatacacc aagttttacg aaaacactta tcaactggtt
     1561 attcttgata ttatgctgcc cggtatgaac gggcatgaac ttttgcgtga atttcgtgcg
     1621 aaaaatgata ctcccattct gatgatgaca gccctgtcgg atgacgaaaa ccaaatccgg
     1681 gcgtttgatg cagaggcaga cgactatgta acaaagccat tcaagatgca gattttacta
     1741 aagcgggtgg aagccctgct gcggcgcagc ggtgcgctgg caaaggaaat ccgtgtcggc
     1801 aggctgacac ttctgccgga ggatttcacg gtactttgtg acggtacaga gctgcccctg
     1861 acacgaaaag aatttgaaat ccttttgctg ctggtgcaga acaaaggcag aaccttaacg
     1921 catgaaatca ttttgtcacg tatatgggga tatgactttg agggtgatgg cagcacagtc
     1981 cacactcata tcaaaaattt gcgggccaag ctgccggaaa atatcatcaa aaccattcgc
     2041 ggtgtaggat accgattgga ggaatcataa taatggaaag aaaagggatt ttcattaagg
     2101 ttttttccta tacgatcatt gtcctgctac tgcttgtcgg tgtaacagca acactgtttg
     2161 cacagcaatt tgtgtcttat ttcagagtga tggaattaca gcaaacagta aaatcctatc
     2221 agccattggt ggaactgatt cagaatagcg ataggcttga tattcaagag gtggcagggc
     2281 tgtttcacta caataaccaa tcctttgagt tttatattga agataaagag ggaagcgtac
     2341 tctatgccac accaaatgcc aatacatcaa atagttttag acccaacttt ctttatgtgg
     2401 tacatagaga tgataatatt tcgattgttg ctcaaagcaa ggcaggtgtg ggattgcttt
     2461 atcaagggct gacaattcgg ggaattgtta tgattgcgat aatggttgta ttcagccttt
     2521 tatgcgcgta tatctttgcg cggcaaatga caacgccgat caaagcctta gcggacagtg
     2581 cgaataaaat ggcaaacctg aaagatgtac cgccgccgct ggagcgaaag gatgagcttg
     2641 gcgcactggc tcatgatatg cattccatgt atgtcaggct gaaagaaacc atcgcaaggc
     2701 tggaggatga aatcgcaagg gaacatgagt tggaggaaac acagcgatat ttctttgcgg
     2761 cagcttctca tgagctaaaa acgcccatcg cggctacaag cgttctgttg gagggaatgc
     2821 ttgaaaatat cggtgactac aaagatcatt ctaagtatct gcgcgaatgc atcaaaatga
     2881 tggataggca gggcaaaatc atttccgaaa tactggagct tgtcagcctg aatgatggga
     2941 gaatcgtacc catagctgaa ccgttggaca tagggcgcac ggttgccgag ttgctgcccg
     3001 attttcaaac cttggcagag gcaaacaacc agcggttcgt cacagatatt ccagccgggc
     3061 aaattgtcct gtccgatccg aggctgctcc aaaaggcact atccaatgtc atattgaatg
     3121 cagttcagaa cacgccgcag ggaggcgagg tacggatatg gagtgagcct ggtgctgaaa
     3181 aatgccgcct ttttgttttg aacatgggcg ttcacattga tgatactgcg cttccaaggc
     3241 tgttcacccc attctatcgc attgatcagg cgcgaagcag aaaaagtggg cgaagcagaa
     3301 aaagtgggcg aagcggttta ggacttgcca tcgtacaaaa aacgctggat gccatgagcc
     3361 tccagtatgc gctggaaaac acctcggatg gcgttttgtt ctggctggat ttaccgctca
     3421 catcaacatt gtaaatattt aaaacttaaa tgattttggg ctctttgtca aataggactg
     3481 atagagtgag ataagaacat ctaaggtaga attccttttc tactttagat gttttttgat
     3541 tcataggtaa tc
//
LOCUS       DAYYNQ010000260         1657 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.203, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000260 DAYYNQ010000000
VERSION     DAYYNQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.203"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACHBJ1_003083"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACHBJ1_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACHBJ1_003084"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACHBJ1_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACHBJ1_003085"
     CDS             complement(1055..1345)
                     /locus_tag="ACHBJ1_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM9431923.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          541 a          310 c          229 g          577 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaa
//
LOCUS       DAYYNQ010000261         1068 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE6376
            SAMEA115708332-rid22599033.guided.231, whole genome shotgun
            sequence.
ACCESSION   DAYYNQ010000261 DAYYNQ010000000
VERSION     DAYYNQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115708332
            Sequence Read Archive: ERR13198057
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290261.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290261.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 17:51:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,087
            CDSs (total)                      :: 3,028
            Genes (coding)                    :: 2,945
            CDSs (with protein)               :: 2,945
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 32 of 83
            Pseudo Genes (incomplete)         :: 38 of 83
            Pseudo Genes (internal stop)      :: 29 of 83
            Pseudo Genes (multiple problems)  :: 13 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115708332-rid22599033.guided.231"
                     /isolate="VRE6376"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHBJ1_003086"
     CDS             110..193
                     /locus_tag="ACHBJ1_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM9431924.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBJ1_003087"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHBJ1_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM9431925.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          406 a          174 c          164 g          324 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgc
//
